Cdk12 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cdk12
Entrez Gene ID	40385
Full Name	Cyclin-dependent kinase 12
Synonyms	AC017581,CDK12,CG7597,Dmel\CG7597,anon-WO0140519.165,cg7597,dCDK12,gi24668141
General protein information	Preferred Names Cyclin-dependent kinase 12 Names CG7597 gene product from transcript CG7597-RB CG7597-PA CG7597-PB CG7597-PC Cdk12-PA Cdk12-PB Cdk12-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 78D4-78D5

mRNA and Protein(s)

mRNA	Protein	Name
NM_168912.2	NP_730643.1	Cyclin-dependent kinase 12, isoform B
NM_001275238.1	NP_001262167.1	Cyclin-dependent kinase 12, isoform C
NM_141068.4	NP_649325.2	Cyclin-dependent kinase 12, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Cdk12 suppresses genes that support metabolic functions in stressed conditions. Cdk12 is not a generally essential Pol II kinase and is not required for the transcription of essential and housekeeping genes at least in adult tissues.
Title: Cdk12 Is A Gene-Selective RNA Polymerase II Kinase That Regulates a Subset of the Transcriptome, Including Nrf2 Target Genes.

CDK12 plays a role in controlling the epigenetic transition between euchromatin and heterochromatin, suggesting a chromatin regulatory mechanism in neuronal behaviors.
Title: Heterochromatin remodeling by CDK12 contributes to learning in Drosophila.

The phosphorylation of the C-terminal domain of the RNA polymerase II by Cdk12 provides an elegant mechanism in regulating timed splicing of newly synthesized mRNA molecules.
Title: The regulation of glial-specific splicing of Neurexin IV requires HOW and Cdk12 activity.

The following Cdk12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cdk12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa19211	NM_001275238.1 Latest version!	Drosophila melanogaster Cyclin-dependent kinase 12 (Cdk12), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa19211	NM_168912.2 Latest version!	Drosophila melanogaster Cyclin-dependent kinase 12 (Cdk12), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa19211	NM_141068.4 Latest version!	Drosophila melanogaster Cyclin-dependent kinase 12 (Cdk12), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cdk12 ( NM_168912.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730643.1 Drosophila melanogaster Cyclin-dependent kinase 12 (Cdk12), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa19211
Clone ID Related Accession (Same CDS sequence)	NM_168912.2 , NM_001275238.1 , NM_141068.4
Accession Version	NM_168912.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Cyclin-dependent kinase 12, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168912.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATGCAT CCTCCGCGGC GGCCACTGCA CTTGTGGAGT ACTCGGACGT CAGCTCAGAG 
GACTTCTCGG ATCAGGAGGC AGGGGACTTG GATGCGGACG CCGGCAAGGG TGCCGGAAAC 
ATCAAAAAAC CGAAGCCAGC TCCGGATAAT CAGTTCTCCA AAGGTCGTCT TGATGCCAAG 
CCCGATAAAG AGGGTTATGA TAACTACAGA AGTAGAAGGG CGGAAGACTC AAGCGACCCA 
GTGGCGGCCG GATCGAGGCA AACGTCTAGC AGCGAGGCCA CGAATCCGCG GGAGGAACCT 
TCGCAGGCAT CCAACACCTC GAAGGACGAG CTGTGGGGCA GAGAGATATA CATGTCCAGC 
GATTCTATCG ACACCGATGA GCTGGAGGCG GAAATGAAGC GGCAGAAGCG CAAAAAGCAG 
AAAAAGGAAA AACACAAACA CAAGTCGAAG AAAAAGTCCA AGAAGCGCAA GAAGAAGCGG 
GCCAAGTCAT ACTCCAGCAT CGATTCGATG TCAGACAATG ACATCAATGC CTTACTGGAC 
CGGCGCTATA CTCCGCCGAC GGCTCCTAGC AAGAGCAACG AACGGACGGT TAGCGCTGCG 
CCGTCCTCCT TCACCCCACA CAACCTCAAG GAGAGCAGTT CGCCAGCCAC TCCACCGCCA 
GTGCGTCGCC CGAATACGAA CAGCAACTAC TACGGCGAGT CGTCCCTGGA GACGGCCAAC 
TCTGCACTGG GAAGCAACCT GCAGGTCACA GTTACGAATA AGCAGTCGAT CAGCAACCGG 
CTTCGTTCTC CTCCTCCGTC CTCCCGCTCC AGCGGCAACG GGCCGAGGTT TGGAAACTCC 
CCGCGAACTC CACCGCCCTC TCACTACAGC TCCAGTGGCG GAGGAGGGGT TGGCAGTGGG 
TCAGTGGTCC GCGATTCACG CAGCTCCCGC TATGTAAATA GTCCCCACAA AGAGGATGTA 
AGTGCGCATC ACCGCTCTAG CCACGATCAC GGATATCAGG GACGTTACTC TGGTGCTGGG 
TCGAGCAGTC ACGATACCAG GAAGGTGAAA CGACTGTCGC CGGAGCTGGA CCGCTACAAC 
CATCAGCCAA GCACGCCACC CCACAAGCGT CGGAAGTTTA GCGATGGCAG GGAAGTGGGC 
CTCGGAAACT TTGAGCACAG TAGACACCAC TCTGGAAAGT ACGAACGATA CAGCAGAGAT 
CGATATTCTA GGCGGTCATC AAGGAGCCCC AGTGTGCAGC ACTCACGAAG TCGGCAATCT 
CCCAGCGGCG GCCTGTCAAG TGGTTCGAAC GCGTTTCGCC ATGGTGGCAG TCACAAACAC 
AAGTATGGGA CCACTGTTAG CTCGACGCCG TCGCATACGA CGAGGACCTC AAAACGCGCT 
TCGGGAACTG GTACCTCTGG CGATCGCTAC TCGAGGTCGC CCAGAACAAG TTCGCGGTAC 
ATGGAGTCAT CACCGCCATC GCCAGTCGGA GCTTCCGGTT CGCATCACTA TCATCATCGC 
CGTTCGCCAA GGATGCGCCA GAGGACCAGA GGCGACAGTC GTCGCCGGTC GCCCAGTTCG 
GCCAGCAGCG AAAGTTCTGC CTCTCGCAGC CGATCACCAA CGTCAAGAGA TCTAAAGCAC 
AAGCGCGAAG AGTACATTAA GAAGATCAGT GAGACTAGCC TATTTGCCGA GCTGGTAAAG 
GATCGTCACA AAAGGCAGAA GGCGCTGAAG GAAATCATTG AGCGGCAAGA GGAGAACAGC 
AATAGCAACA GCAACGGTGC ACTGACTATT AACGACAACA GTTCCTCCGT GGATGGCAAT 
ACACCCAACG CGGCTGATGG CCGATCTGCC CCTGGAAGTG GAACACCAGC AGCGGCGTCA 
ACAACTTCCA ATGGGCTTCA AGCTTTGGGT AGCAAGCCGG ATCTGGACCT CAACAACATT 
CCCATGCCAA ACAAGCAGAA CGATTCTGTG GTTTCGAATC CGGCTTCAAA CGCCGATGTT 
CCGGACAGTG TCGCGCAGTT GAAGCAACCT TTGCTGGTGC CACCGTTCTC GGCTAGTAAG 
AACAACATAA AACCGAAGAG CCTGACTTCT TTGCCGCTGC CGCCGGGTAT GAATGTGCTC 
GACTTGGCCG GTGCAAGGTC TCCCTCTCCG GGGCAAAAGA AGGAATCGGA CGAAAAGAAC 
GTTACTTCAA GCGGCAGCGC CAACAAGAGC GTGCTCAATC TTCCTATGCC GCCAGTAATT 
CCCGGCAGCG AAGAGCTTAG TGGCGATGAT GATGTTATCG ACAGTCCTGA AGACTTTGAT 
GCACCCGCGG TTGGCACTGT GCACGGTCAC GGTGGTGGGC CAGGGACAAC TCGTCAGCGC 
CCAGTGATAC TCAACCGTCG TGACTCGCGA AACAACGTAC GCGACTGGGG CGAGCGCTGT 
GTGGATGTTT TTGAGATGAT TGCGCAAATA GGGGAAGGAA CTTACGGACA GGTTTACAAG 
GCGCGGGATC ATCATACCAA TGACATGGTA GCACTGAAGA AGGTTCGGCT GGAGCACGAG 
AAGGAGGGCT TTCCCATTAC AGCTGTTCGT GAAATCAAAA TTCTGAGACA ACTTAACCAT 
CGCAATATAG TCAACCTGCA TGAAATTGTG ACGGACAAGC AGGATGCTGT GGAGTTCCGT 
AAAGACAAGG GATCCTTCTA CTTGGTGTTT GAGTACATGG ATCACGACTT GATGGGTCTG 
CTGGAGTCTG GCATGGTCGA CTTCAACGAG GAAAACAACG CAAGCATTAT GAAGCAGCTC 
TTGGACGGCC TCAACTACTG CCACAAAAAG AATTTTCTGC ATCGAGACAT CAAGTGCTCG 
AACATTTTAA TGAACAACAG GGGTAAAGTA AAACTAGCTG ACTTTGGGCT GGCACGACTA 
TACAATGCGG ACGATCGGGA ACGCCCCTAC ACGAACAAAG TAATTACCTT GTGGTATCGT 
CCTCCTGAGC TTTTGCTGGG TGAGGAGCGT TACGGCCCGT CGATAGACGT GTGGTCCTGC 
GGGTGCATCC TCGGCGAACT CTTTGTGAAG CGGCCCCTGT TCCAGGCTAA TGCGGAAATG 
GCGCAGCTTG AAACGATATC AAAGATCTGT GGATCCCCGG TTCCTGCCGT GTGGCCGAAT 
GTAATTAAGC TGCCTCTGTT CCACACTCTC AAACAAAAGA AGACGCACCG CCGCCGTCTA 
CGAGAAGACT TTGAATTCAT GCCAGCCCCT GCACTTGATC TGCTGGACAA AATGCTTGAT 
TTAGATCCGG ACAAGCGCAT CACAGCAGAA GACGCGCTCC GGTCACCGTG GCTAAGGAAA 
ATCAACCCCG ACGAAATGCC AACGCCACAA TTGCCCACAT GGCAGGACTG CCATGAGCTT 
TGGAGTAAGA AGCGCCGTCG GCAGATGCGG GAGCAACAGG AGTCGCTTCC TCCTACGGTG 
ATAGCCTCGA CCAAATACCA ACAGCATGGA GCAACCATGG TGGGGGATGC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730643.1
CDS	682..4155
Translation	MHASSAAATALVEYSDVSSEDFSDQEAGDLDADAGKGAGNIKKPKPAPDNQFSKGRLDAKPDKEGYDNYRSRRAEDSSDPVAAGSRQTSSSEATNPREEPSQASNTSKDELWGREIYMSSDSIDTDELEAEMKRQKRKKQKKEKHKHKSKKKSKKRKKKRAKSYSSIDSMSDNDINALLDRRYTPPTAPSKSNERTVSAAPSSFTPHNLKESSSPATPPPVRRPNTNSNYYGESSLETANSALGSNLQVTVTNKQSISNRLRSPPPSSRSSGNGPRFGNSPRTPPPSHYSSSGGGGVGSGSVVRDSRSSRYVNSPHKEDVSAHHRSSHDHGYQGRYSGAGSSSHDTRKVKRLSPELDRYNHQPSTPPHKRRKFSDGREVGLGNFEHSRHHSGKYERYSRDRYSRRSSRSPSVQHSRSRQSPSGGLSSGSNAFRHGGSHKHKYGTTVSSTPSHTTRTSKRASGTGTSGDRYSRSPRTSSRYMESSPPSPVGASGSHHYHHRRSPRMRQRTRGDSRRRSPSSASSESSASRSRSPTSRDLKHKREEYIKKISETSLFAELVKDRHKRQKALKEIIERQEENSNSNSNGALTINDNSSSVDGNTPNAADGRSAPGSGTPAAASTTSNGLQALGSKPDLDLNNIPMPNKQNDSVVSNPASNADVPDSVAQLKQPLLVPPFSASKNNIKPKSLTSLPLPPGMNVLDLAGARSPSPGQKKESDEKNVTSSGSANKSVLNLPMPPVIPGSEELSGDDDVIDSPEDFDAPAVGTVHGHGGGPGTTRQRPVILNRRDSRNNVRDWGERCVDVFEMIAQIGEGTYGQVYKARDHHTNDMVALKKVRLEHEKEGFPITAVREIKILRQLNHRNIVNLHEIVTDKQDAVEFRKDKGSFYLVFEYMDHDLMGLLESGMVDFNEENNASIMKQLLDGLNYCHKKNFLHRDIKCSNILMNNRGKVKLADFGLARLYNADDRERPYTNKVITLWYRPPELLLGEERYGPSIDVWSCGCILGELFVKRPLFQANAEMAQLETISKICGSPVPAVWPNVIKLPLFHTLKQKKTHRRRLREDFEFMPAPALDLLDKMLDLDPDKRITAEDALRSPWLRKINPDEMPTPQLPTWQDCHELWSKKRRRQMREQQESLPPTVIASTKYQQHGATMVGDA

Target ORF information:

RefSeq Version	NM_168912.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Cyclin-dependent kinase 12 (Cdk12), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168912.2

ORF Insert Sequence:

ATGCATGCAT CCTCCGCGGC GGCCACTGCA CTTGTGGAGT ACTCGGACGT CAGCTCAGAG 
GACTTCTCGG ATCAGGAGGC AGGGGACTTG GATGCGGACG CCGGCAAGGG TGCCGGAAAC 
ATCAAAAAAC CGAAGCCAGC TCCGGATAAT CAGTTCTCCA AAGGTCGTCT TGATGCCAAG 
CCCGATAAAG AGGGTTATGA TAACTACAGA AGTAGAAGGG CGGAAGACTC AAGCGACCCA 
GTGGCGGCCG GATCGAGGCA AACGTCTAGC AGCGAGGCCA CGAATCCGCG GGAGGAACCT 
TCGCAGGCAT CCAACACCTC GAAGGACGAG CTGTGGGGCA GAGAGATATA CATGTCCAGC 
GATTCTATCG ACACCGATGA GCTGGAGGCG GAAATGAAGC GGCAGAAGCG CAAAAAGCAG 
AAAAAGGAAA AACACAAACA CAAGTCGAAG AAAAAGTCCA AGAAGCGCAA GAAGAAGCGG 
GCCAAGTCAT ACTCCAGCAT CGATTCGATG TCAGACAATG ACATCAATGC CTTACTGGAC 
CGGCGCTATA CTCCGCCGAC GGCTCCTAGC AAGAGCAACG AACGGACGGT TAGCGCTGCG 
CCGTCCTCCT TCACCCCACA CAACCTCAAG GAGAGCAGTT CGCCAGCCAC TCCACCGCCA 
GTGCGTCGCC CGAATACGAA CAGCAACTAC TACGGCGAGT CGTCCCTGGA GACGGCCAAC 
TCTGCACTGG GAAGCAACCT GCAGGTCACA GTTACGAATA AGCAGTCGAT CAGCAACCGG 
CTTCGTTCTC CTCCTCCGTC CTCCCGCTCC AGCGGCAACG GGCCGAGGTT TGGAAACTCC 
CCGCGAACTC CACCGCCCTC TCACTACAGC TCCAGTGGCG GAGGAGGGGT TGGCAGTGGG 
TCAGTGGTCC GCGATTCACG CAGCTCCCGC TATGTAAATA GTCCCCACAA AGAGGATGTA 
AGTGCGCATC ACCGCTCTAG CCACGATCAC GGATATCAGG GACGTTACTC TGGTGCTGGG 
TCGAGCAGTC ACGATACCAG GAAGGTGAAA CGACTGTCGC CGGAGCTGGA CCGCTACAAC 
CATCAGCCAA GCACGCCACC CCACAAGCGT CGGAAGTTTA GCGATGGCAG GGAAGTGGGC 
CTCGGAAACT TTGAGCACAG TAGACACCAC TCTGGAAAGT ACGAACGATA CAGCAGAGAT 
CGATATTCTA GGCGGTCATC AAGGAGCCCC AGTGTGCAGC ACTCACGAAG TCGGCAATCT 
CCCAGCGGCG GCCTGTCAAG TGGTTCGAAC GCGTTTCGCC ATGGTGGCAG TCACAAACAC 
AAGTATGGGA CCACTGTTAG CTCGACGCCG TCGCATACGA CGAGGACCTC AAAACGCGCT 
TCGGGAACTG GTACCTCTGG CGATCGCTAC TCGAGGTCGC CCAGAACAAG TTCGCGGTAC 
ATGGAGTCAT CACCGCCATC GCCAGTCGGA GCTTCCGGTT CGCATCACTA TCATCATCGC 
CGTTCGCCAA GGATGCGCCA GAGGACCAGA GGCGACAGTC GTCGCCGGTC GCCCAGTTCG 
GCCAGCAGCG AAAGTTCTGC CTCTCGCAGC CGATCACCAA CGTCAAGAGA TCTAAAGCAC 
AAGCGCGAAG AGTACATTAA GAAGATCAGT GAGACTAGCC TATTTGCCGA GCTGGTAAAG 
GATCGTCACA AAAGGCAGAA GGCGCTGAAG GAAATCATTG AGCGGCAAGA GGAGAACAGC 
AATAGCAACA GCAACGGTGC ACTGACTATT AACGACAACA GTTCCTCCGT GGATGGCAAT 
ACACCCAACG CGGCTGATGG CCGATCTGCC CCTGGAAGTG GAACACCAGC AGCGGCGTCA 
ACAACTTCCA ATGGGCTTCA AGCTTTGGGT AGCAAGCCGG ATCTGGACCT CAACAACATT 
CCCATGCCAA ACAAGCAGAA CGATTCTGTG GTTTCGAATC CGGCTTCAAA CGCCGATGTT 
CCGGACAGTG TCGCGCAGTT GAAGCAACCT TTGCTGGTGC CACCGTTCTC GGCTAGTAAG 
AACAACATAA AACCGAAGAG CCTGACTTCT TTGCCGCTGC CGCCGGGTAT GAATGTGCTC 
GACTTGGCCG GTGCAAGGTC TCCCTCTCCG GGGCAAAAGA AGGAATCGGA CGAAAAGAAC 
GTTACTTCAA GCGGCAGCGC CAACAAGAGC GTGCTCAATC TTCCTATGCC GCCAGTAATT 
CCCGGCAGCG AAGAGCTTAG TGGCGATGAT GATGTTATCG ACAGTCCTGA AGACTTTGAT 
GCACCCGCGG TTGGCACTGT GCACGGTCAC GGTGGTGGGC CAGGGACAAC TCGTCAGCGC 
CCAGTGATAC TCAACCGTCG TGACTCGCGA AACAACGTAC GCGACTGGGG CGAGCGCTGT 
GTGGATGTTT TTGAGATGAT TGCGCAAATA GGGGAAGGAA CTTACGGACA GGTTTACAAG 
GCGCGGGATC ATCATACCAA TGACATGGTA GCACTGAAGA AGGTTCGGCT GGAGCACGAG 
AAGGAGGGCT TTCCCATTAC AGCTGTTCGT GAAATCAAAA TTCTGAGACA ACTTAACCAT 
CGCAATATAG TCAACCTGCA TGAAATTGTG ACGGACAAGC AGGATGCTGT GGAGTTCCGT 
AAAGACAAGG GATCCTTCTA CTTGGTGTTT GAGTACATGG ATCACGACTT GATGGGTCTG 
CTGGAGTCTG GCATGGTCGA CTTCAACGAG GAAAACAACG CAAGCATTAT GAAGCAGCTC 
TTGGACGGCC TCAACTACTG CCACAAAAAG AATTTTCTGC ATCGAGACAT CAAGTGCTCG 
AACATTTTAA TGAACAACAG GGGTAAAGTA AAACTAGCTG ACTTTGGGCT GGCACGACTA 
TACAATGCGG ACGATCGGGA ACGCCCCTAC ACGAACAAAG TAATTACCTT GTGGTATCGT 
CCTCCTGAGC TTTTGCTGGG TGAGGAGCGT TACGGCCCGT CGATAGACGT GTGGTCCTGC 
GGGTGCATCC TCGGCGAACT CTTTGTGAAG CGGCCCCTGT TCCAGGCTAA TGCGGAAATG 
GCGCAGCTTG AAACGATATC AAAGATCTGT GGATCCCCGG TTCCTGCCGT GTGGCCGAAT 
GTAATTAAGC TGCCTCTGTT CCACACTCTC AAACAAAAGA AGACGCACCG CCGCCGTCTA 
CGAGAAGACT TTGAATTCAT GCCAGCCCCT GCACTTGATC TGCTGGACAA AATGCTTGAT 
TTAGATCCGG ACAAGCGCAT CACAGCAGAA GACGCGCTCC GGTCACCGTG GCTAAGGAAA 
ATCAACCCCG ACGAAATGCC AACGCCACAA TTGCCCACAT GGCAGGACTG CCATGAGCTT 
TGGAGTAAGA AGCGCCGTCG GCAGATGCGG GAGCAACAGG AGTCGCTTCC TCCTACGGTG 
ATAGCCTCGA CCAAATACCA ACAGCATGGA GCAACCATGG TGGGGGATGC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdk12 ( NM_001275238.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262167.1 Drosophila melanogaster Cyclin-dependent kinase 12 (Cdk12), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa19211
Clone ID Related Accession (Same CDS sequence)	NM_168912.2 , NM_001275238.1 , NM_141068.4
Accession Version	NM_001275238.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Cyclin-dependent kinase 12, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATGCAT CCTCCGCGGC GGCCACTGCA CTTGTGGAGT ACTCGGACGT CAGCTCAGAG 
GACTTCTCGG ATCAGGAGGC AGGGGACTTG GATGCGGACG CCGGCAAGGG TGCCGGAAAC 
ATCAAAAAAC CGAAGCCAGC TCCGGATAAT CAGTTCTCCA AAGGTCGTCT TGATGCCAAG 
CCCGATAAAG AGGGTTATGA TAACTACAGA AGTAGAAGGG CGGAAGACTC AAGCGACCCA 
GTGGCGGCCG GATCGAGGCA AACGTCTAGC AGCGAGGCCA CGAATCCGCG GGAGGAACCT 
TCGCAGGCAT CCAACACCTC GAAGGACGAG CTGTGGGGCA GAGAGATATA CATGTCCAGC 
GATTCTATCG ACACCGATGA GCTGGAGGCG GAAATGAAGC GGCAGAAGCG CAAAAAGCAG 
AAAAAGGAAA AACACAAACA CAAGTCGAAG AAAAAGTCCA AGAAGCGCAA GAAGAAGCGG 
GCCAAGTCAT ACTCCAGCAT CGATTCGATG TCAGACAATG ACATCAATGC CTTACTGGAC 
CGGCGCTATA CTCCGCCGAC GGCTCCTAGC AAGAGCAACG AACGGACGGT TAGCGCTGCG 
CCGTCCTCCT TCACCCCACA CAACCTCAAG GAGAGCAGTT CGCCAGCCAC TCCACCGCCA 
GTGCGTCGCC CGAATACGAA CAGCAACTAC TACGGCGAGT CGTCCCTGGA GACGGCCAAC 
TCTGCACTGG GAAGCAACCT GCAGGTCACA GTTACGAATA AGCAGTCGAT CAGCAACCGG 
CTTCGTTCTC CTCCTCCGTC CTCCCGCTCC AGCGGCAACG GGCCGAGGTT TGGAAACTCC 
CCGCGAACTC CACCGCCCTC TCACTACAGC TCCAGTGGCG GAGGAGGGGT TGGCAGTGGG 
TCAGTGGTCC GCGATTCACG CAGCTCCCGC TATGTAAATA GTCCCCACAA AGAGGATGTA 
AGTGCGCATC ACCGCTCTAG CCACGATCAC GGATATCAGG GACGTTACTC TGGTGCTGGG 
TCGAGCAGTC ACGATACCAG GAAGGTGAAA CGACTGTCGC CGGAGCTGGA CCGCTACAAC 
CATCAGCCAA GCACGCCACC CCACAAGCGT CGGAAGTTTA GCGATGGCAG GGAAGTGGGC 
CTCGGAAACT TTGAGCACAG TAGACACCAC TCTGGAAAGT ACGAACGATA CAGCAGAGAT 
CGATATTCTA GGCGGTCATC AAGGAGCCCC AGTGTGCAGC ACTCACGAAG TCGGCAATCT 
CCCAGCGGCG GCCTGTCAAG TGGTTCGAAC GCGTTTCGCC ATGGTGGCAG TCACAAACAC 
AAGTATGGGA CCACTGTTAG CTCGACGCCG TCGCATACGA CGAGGACCTC AAAACGCGCT 
TCGGGAACTG GTACCTCTGG CGATCGCTAC TCGAGGTCGC CCAGAACAAG TTCGCGGTAC 
ATGGAGTCAT CACCGCCATC GCCAGTCGGA GCTTCCGGTT CGCATCACTA TCATCATCGC 
CGTTCGCCAA GGATGCGCCA GAGGACCAGA GGCGACAGTC GTCGCCGGTC GCCCAGTTCG 
GCCAGCAGCG AAAGTTCTGC CTCTCGCAGC CGATCACCAA CGTCAAGAGA TCTAAAGCAC 
AAGCGCGAAG AGTACATTAA GAAGATCAGT GAGACTAGCC TATTTGCCGA GCTGGTAAAG 
GATCGTCACA AAAGGCAGAA GGCGCTGAAG GAAATCATTG AGCGGCAAGA GGAGAACAGC 
AATAGCAACA GCAACGGTGC ACTGACTATT AACGACAACA GTTCCTCCGT GGATGGCAAT 
ACACCCAACG CGGCTGATGG CCGATCTGCC CCTGGAAGTG GAACACCAGC AGCGGCGTCA 
ACAACTTCCA ATGGGCTTCA AGCTTTGGGT AGCAAGCCGG ATCTGGACCT CAACAACATT 
CCCATGCCAA ACAAGCAGAA CGATTCTGTG GTTTCGAATC CGGCTTCAAA CGCCGATGTT 
CCGGACAGTG TCGCGCAGTT GAAGCAACCT TTGCTGGTGC CACCGTTCTC GGCTAGTAAG 
AACAACATAA AACCGAAGAG CCTGACTTCT TTGCCGCTGC CGCCGGGTAT GAATGTGCTC 
GACTTGGCCG GTGCAAGGTC TCCCTCTCCG GGGCAAAAGA AGGAATCGGA CGAAAAGAAC 
GTTACTTCAA GCGGCAGCGC CAACAAGAGC GTGCTCAATC TTCCTATGCC GCCAGTAATT 
CCCGGCAGCG AAGAGCTTAG TGGCGATGAT GATGTTATCG ACAGTCCTGA AGACTTTGAT 
GCACCCGCGG TTGGCACTGT GCACGGTCAC GGTGGTGGGC CAGGGACAAC TCGTCAGCGC 
CCAGTGATAC TCAACCGTCG TGACTCGCGA AACAACGTAC GCGACTGGGG CGAGCGCTGT 
GTGGATGTTT TTGAGATGAT TGCGCAAATA GGGGAAGGAA CTTACGGACA GGTTTACAAG 
GCGCGGGATC ATCATACCAA TGACATGGTA GCACTGAAGA AGGTTCGGCT GGAGCACGAG 
AAGGAGGGCT TTCCCATTAC AGCTGTTCGT GAAATCAAAA TTCTGAGACA ACTTAACCAT 
CGCAATATAG TCAACCTGCA TGAAATTGTG ACGGACAAGC AGGATGCTGT GGAGTTCCGT 
AAAGACAAGG GATCCTTCTA CTTGGTGTTT GAGTACATGG ATCACGACTT GATGGGTCTG 
CTGGAGTCTG GCATGGTCGA CTTCAACGAG GAAAACAACG CAAGCATTAT GAAGCAGCTC 
TTGGACGGCC TCAACTACTG CCACAAAAAG AATTTTCTGC ATCGAGACAT CAAGTGCTCG 
AACATTTTAA TGAACAACAG GGGTAAAGTA AAACTAGCTG ACTTTGGGCT GGCACGACTA 
TACAATGCGG ACGATCGGGA ACGCCCCTAC ACGAACAAAG TAATTACCTT GTGGTATCGT 
CCTCCTGAGC TTTTGCTGGG TGAGGAGCGT TACGGCCCGT CGATAGACGT GTGGTCCTGC 
GGGTGCATCC TCGGCGAACT CTTTGTGAAG CGGCCCCTGT TCCAGGCTAA TGCGGAAATG 
GCGCAGCTTG AAACGATATC AAAGATCTGT GGATCCCCGG TTCCTGCCGT GTGGCCGAAT 
GTAATTAAGC TGCCTCTGTT CCACACTCTC AAACAAAAGA AGACGCACCG CCGCCGTCTA 
CGAGAAGACT TTGAATTCAT GCCAGCCCCT GCACTTGATC TGCTGGACAA AATGCTTGAT 
TTAGATCCGG ACAAGCGCAT CACAGCAGAA GACGCGCTCC GGTCACCGTG GCTAAGGAAA 
ATCAACCCCG ACGAAATGCC AACGCCACAA TTGCCCACAT GGCAGGACTG CCATGAGCTT 
TGGAGTAAGA AGCGCCGTCG GCAGATGCGG GAGCAACAGG AGTCGCTTCC TCCTACGGTG 
ATAGCCTCGA CCAAATACCA ACAGCATGGA GCAACCATGG TGGGGGATGC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262167.1
CDS	396..3869
Translation	MHASSAAATALVEYSDVSSEDFSDQEAGDLDADAGKGAGNIKKPKPAPDNQFSKGRLDAKPDKEGYDNYRSRRAEDSSDPVAAGSRQTSSSEATNPREEPSQASNTSKDELWGREIYMSSDSIDTDELEAEMKRQKRKKQKKEKHKHKSKKKSKKRKKKRAKSYSSIDSMSDNDINALLDRRYTPPTAPSKSNERTVSAAPSSFTPHNLKESSSPATPPPVRRPNTNSNYYGESSLETANSALGSNLQVTVTNKQSISNRLRSPPPSSRSSGNGPRFGNSPRTPPPSHYSSSGGGGVGSGSVVRDSRSSRYVNSPHKEDVSAHHRSSHDHGYQGRYSGAGSSSHDTRKVKRLSPELDRYNHQPSTPPHKRRKFSDGREVGLGNFEHSRHHSGKYERYSRDRYSRRSSRSPSVQHSRSRQSPSGGLSSGSNAFRHGGSHKHKYGTTVSSTPSHTTRTSKRASGTGTSGDRYSRSPRTSSRYMESSPPSPVGASGSHHYHHRRSPRMRQRTRGDSRRRSPSSASSESSASRSRSPTSRDLKHKREEYIKKISETSLFAELVKDRHKRQKALKEIIERQEENSNSNSNGALTINDNSSSVDGNTPNAADGRSAPGSGTPAAASTTSNGLQALGSKPDLDLNNIPMPNKQNDSVVSNPASNADVPDSVAQLKQPLLVPPFSASKNNIKPKSLTSLPLPPGMNVLDLAGARSPSPGQKKESDEKNVTSSGSANKSVLNLPMPPVIPGSEELSGDDDVIDSPEDFDAPAVGTVHGHGGGPGTTRQRPVILNRRDSRNNVRDWGERCVDVFEMIAQIGEGTYGQVYKARDHHTNDMVALKKVRLEHEKEGFPITAVREIKILRQLNHRNIVNLHEIVTDKQDAVEFRKDKGSFYLVFEYMDHDLMGLLESGMVDFNEENNASIMKQLLDGLNYCHKKNFLHRDIKCSNILMNNRGKVKLADFGLARLYNADDRERPYTNKVITLWYRPPELLLGEERYGPSIDVWSCGCILGELFVKRPLFQANAEMAQLETISKICGSPVPAVWPNVIKLPLFHTLKQKKTHRRRLREDFEFMPAPALDLLDKMLDLDPDKRITAEDALRSPWLRKINPDEMPTPQLPTWQDCHELWSKKRRRQMREQQESLPPTVIASTKYQQHGATMVGDA

Target ORF information:

RefSeq Version	NM_001275238.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Cyclin-dependent kinase 12 (Cdk12), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275238.1

ORF Insert Sequence:

ATGCATGCAT CCTCCGCGGC GGCCACTGCA CTTGTGGAGT ACTCGGACGT CAGCTCAGAG 
GACTTCTCGG ATCAGGAGGC AGGGGACTTG GATGCGGACG CCGGCAAGGG TGCCGGAAAC 
ATCAAAAAAC CGAAGCCAGC TCCGGATAAT CAGTTCTCCA AAGGTCGTCT TGATGCCAAG 
CCCGATAAAG AGGGTTATGA TAACTACAGA AGTAGAAGGG CGGAAGACTC AAGCGACCCA 
GTGGCGGCCG GATCGAGGCA AACGTCTAGC AGCGAGGCCA CGAATCCGCG GGAGGAACCT 
TCGCAGGCAT CCAACACCTC GAAGGACGAG CTGTGGGGCA GAGAGATATA CATGTCCAGC 
GATTCTATCG ACACCGATGA GCTGGAGGCG GAAATGAAGC GGCAGAAGCG CAAAAAGCAG 
AAAAAGGAAA AACACAAACA CAAGTCGAAG AAAAAGTCCA AGAAGCGCAA GAAGAAGCGG 
GCCAAGTCAT ACTCCAGCAT CGATTCGATG TCAGACAATG ACATCAATGC CTTACTGGAC 
CGGCGCTATA CTCCGCCGAC GGCTCCTAGC AAGAGCAACG AACGGACGGT TAGCGCTGCG 
CCGTCCTCCT TCACCCCACA CAACCTCAAG GAGAGCAGTT CGCCAGCCAC TCCACCGCCA 
GTGCGTCGCC CGAATACGAA CAGCAACTAC TACGGCGAGT CGTCCCTGGA GACGGCCAAC 
TCTGCACTGG GAAGCAACCT GCAGGTCACA GTTACGAATA AGCAGTCGAT CAGCAACCGG 
CTTCGTTCTC CTCCTCCGTC CTCCCGCTCC AGCGGCAACG GGCCGAGGTT TGGAAACTCC 
CCGCGAACTC CACCGCCCTC TCACTACAGC TCCAGTGGCG GAGGAGGGGT TGGCAGTGGG 
TCAGTGGTCC GCGATTCACG CAGCTCCCGC TATGTAAATA GTCCCCACAA AGAGGATGTA 
AGTGCGCATC ACCGCTCTAG CCACGATCAC GGATATCAGG GACGTTACTC TGGTGCTGGG 
TCGAGCAGTC ACGATACCAG GAAGGTGAAA CGACTGTCGC CGGAGCTGGA CCGCTACAAC 
CATCAGCCAA GCACGCCACC CCACAAGCGT CGGAAGTTTA GCGATGGCAG GGAAGTGGGC 
CTCGGAAACT TTGAGCACAG TAGACACCAC TCTGGAAAGT ACGAACGATA CAGCAGAGAT 
CGATATTCTA GGCGGTCATC AAGGAGCCCC AGTGTGCAGC ACTCACGAAG TCGGCAATCT 
CCCAGCGGCG GCCTGTCAAG TGGTTCGAAC GCGTTTCGCC ATGGTGGCAG TCACAAACAC 
AAGTATGGGA CCACTGTTAG CTCGACGCCG TCGCATACGA CGAGGACCTC AAAACGCGCT 
TCGGGAACTG GTACCTCTGG CGATCGCTAC TCGAGGTCGC CCAGAACAAG TTCGCGGTAC 
ATGGAGTCAT CACCGCCATC GCCAGTCGGA GCTTCCGGTT CGCATCACTA TCATCATCGC 
CGTTCGCCAA GGATGCGCCA GAGGACCAGA GGCGACAGTC GTCGCCGGTC GCCCAGTTCG 
GCCAGCAGCG AAAGTTCTGC CTCTCGCAGC CGATCACCAA CGTCAAGAGA TCTAAAGCAC 
AAGCGCGAAG AGTACATTAA GAAGATCAGT GAGACTAGCC TATTTGCCGA GCTGGTAAAG 
GATCGTCACA AAAGGCAGAA GGCGCTGAAG GAAATCATTG AGCGGCAAGA GGAGAACAGC 
AATAGCAACA GCAACGGTGC ACTGACTATT AACGACAACA GTTCCTCCGT GGATGGCAAT 
ACACCCAACG CGGCTGATGG CCGATCTGCC CCTGGAAGTG GAACACCAGC AGCGGCGTCA 
ACAACTTCCA ATGGGCTTCA AGCTTTGGGT AGCAAGCCGG ATCTGGACCT CAACAACATT 
CCCATGCCAA ACAAGCAGAA CGATTCTGTG GTTTCGAATC CGGCTTCAAA CGCCGATGTT 
CCGGACAGTG TCGCGCAGTT GAAGCAACCT TTGCTGGTGC CACCGTTCTC GGCTAGTAAG 
AACAACATAA AACCGAAGAG CCTGACTTCT TTGCCGCTGC CGCCGGGTAT GAATGTGCTC 
GACTTGGCCG GTGCAAGGTC TCCCTCTCCG GGGCAAAAGA AGGAATCGGA CGAAAAGAAC 
GTTACTTCAA GCGGCAGCGC CAACAAGAGC GTGCTCAATC TTCCTATGCC GCCAGTAATT 
CCCGGCAGCG AAGAGCTTAG TGGCGATGAT GATGTTATCG ACAGTCCTGA AGACTTTGAT 
GCACCCGCGG TTGGCACTGT GCACGGTCAC GGTGGTGGGC CAGGGACAAC TCGTCAGCGC 
CCAGTGATAC TCAACCGTCG TGACTCGCGA AACAACGTAC GCGACTGGGG CGAGCGCTGT 
GTGGATGTTT TTGAGATGAT TGCGCAAATA GGGGAAGGAA CTTACGGACA GGTTTACAAG 
GCGCGGGATC ATCATACCAA TGACATGGTA GCACTGAAGA AGGTTCGGCT GGAGCACGAG 
AAGGAGGGCT TTCCCATTAC AGCTGTTCGT GAAATCAAAA TTCTGAGACA ACTTAACCAT 
CGCAATATAG TCAACCTGCA TGAAATTGTG ACGGACAAGC AGGATGCTGT GGAGTTCCGT 
AAAGACAAGG GATCCTTCTA CTTGGTGTTT GAGTACATGG ATCACGACTT GATGGGTCTG 
CTGGAGTCTG GCATGGTCGA CTTCAACGAG GAAAACAACG CAAGCATTAT GAAGCAGCTC 
TTGGACGGCC TCAACTACTG CCACAAAAAG AATTTTCTGC ATCGAGACAT CAAGTGCTCG 
AACATTTTAA TGAACAACAG GGGTAAAGTA AAACTAGCTG ACTTTGGGCT GGCACGACTA 
TACAATGCGG ACGATCGGGA ACGCCCCTAC ACGAACAAAG TAATTACCTT GTGGTATCGT 
CCTCCTGAGC TTTTGCTGGG TGAGGAGCGT TACGGCCCGT CGATAGACGT GTGGTCCTGC 
GGGTGCATCC TCGGCGAACT CTTTGTGAAG CGGCCCCTGT TCCAGGCTAA TGCGGAAATG 
GCGCAGCTTG AAACGATATC AAAGATCTGT GGATCCCCGG TTCCTGCCGT GTGGCCGAAT 
GTAATTAAGC TGCCTCTGTT CCACACTCTC AAACAAAAGA AGACGCACCG CCGCCGTCTA 
CGAGAAGACT TTGAATTCAT GCCAGCCCCT GCACTTGATC TGCTGGACAA AATGCTTGAT 
TTAGATCCGG ACAAGCGCAT CACAGCAGAA GACGCGCTCC GGTCACCGTG GCTAAGGAAA 
ATCAACCCCG ACGAAATGCC AACGCCACAA TTGCCCACAT GGCAGGACTG CCATGAGCTT 
TGGAGTAAGA AGCGCCGTCG GCAGATGCGG GAGCAACAGG AGTCGCTTCC TCCTACGGTG 
ATAGCCTCGA CCAAATACCA ACAGCATGGA GCAACCATGG TGGGGGATGC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdk12 ( NM_141068.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649325.2 Drosophila melanogaster Cyclin-dependent kinase 12 (Cdk12), transcript variant A, mRNA.

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CloneID	OFa19211
Clone ID Related Accession (Same CDS sequence)	NM_168912.2 , NM_001275238.1 , NM_141068.4
Accession Version	NM_141068.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Cyclin-dependent kinase 12, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_141068.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATGCAT CCTCCGCGGC GGCCACTGCA CTTGTGGAGT ACTCGGACGT CAGCTCAGAG 
GACTTCTCGG ATCAGGAGGC AGGGGACTTG GATGCGGACG CCGGCAAGGG TGCCGGAAAC 
ATCAAAAAAC CGAAGCCAGC TCCGGATAAT CAGTTCTCCA AAGGTCGTCT TGATGCCAAG 
CCCGATAAAG AGGGTTATGA TAACTACAGA AGTAGAAGGG CGGAAGACTC AAGCGACCCA 
GTGGCGGCCG GATCGAGGCA AACGTCTAGC AGCGAGGCCA CGAATCCGCG GGAGGAACCT 
TCGCAGGCAT CCAACACCTC GAAGGACGAG CTGTGGGGCA GAGAGATATA CATGTCCAGC 
GATTCTATCG ACACCGATGA GCTGGAGGCG GAAATGAAGC GGCAGAAGCG CAAAAAGCAG 
AAAAAGGAAA AACACAAACA CAAGTCGAAG AAAAAGTCCA AGAAGCGCAA GAAGAAGCGG 
GCCAAGTCAT ACTCCAGCAT CGATTCGATG TCAGACAATG ACATCAATGC CTTACTGGAC 
CGGCGCTATA CTCCGCCGAC GGCTCCTAGC AAGAGCAACG AACGGACGGT TAGCGCTGCG 
CCGTCCTCCT TCACCCCACA CAACCTCAAG GAGAGCAGTT CGCCAGCCAC TCCACCGCCA 
GTGCGTCGCC CGAATACGAA CAGCAACTAC TACGGCGAGT CGTCCCTGGA GACGGCCAAC 
TCTGCACTGG GAAGCAACCT GCAGGTCACA GTTACGAATA AGCAGTCGAT CAGCAACCGG 
CTTCGTTCTC CTCCTCCGTC CTCCCGCTCC AGCGGCAACG GGCCGAGGTT TGGAAACTCC 
CCGCGAACTC CACCGCCCTC TCACTACAGC TCCAGTGGCG GAGGAGGGGT TGGCAGTGGG 
TCAGTGGTCC GCGATTCACG CAGCTCCCGC TATGTAAATA GTCCCCACAA AGAGGATGTA 
AGTGCGCATC ACCGCTCTAG CCACGATCAC GGATATCAGG GACGTTACTC TGGTGCTGGG 
TCGAGCAGTC ACGATACCAG GAAGGTGAAA CGACTGTCGC CGGAGCTGGA CCGCTACAAC 
CATCAGCCAA GCACGCCACC CCACAAGCGT CGGAAGTTTA GCGATGGCAG GGAAGTGGGC 
CTCGGAAACT TTGAGCACAG TAGACACCAC TCTGGAAAGT ACGAACGATA CAGCAGAGAT 
CGATATTCTA GGCGGTCATC AAGGAGCCCC AGTGTGCAGC ACTCACGAAG TCGGCAATCT 
CCCAGCGGCG GCCTGTCAAG TGGTTCGAAC GCGTTTCGCC ATGGTGGCAG TCACAAACAC 
AAGTATGGGA CCACTGTTAG CTCGACGCCG TCGCATACGA CGAGGACCTC AAAACGCGCT 
TCGGGAACTG GTACCTCTGG CGATCGCTAC TCGAGGTCGC CCAGAACAAG TTCGCGGTAC 
ATGGAGTCAT CACCGCCATC GCCAGTCGGA GCTTCCGGTT CGCATCACTA TCATCATCGC 
CGTTCGCCAA GGATGCGCCA GAGGACCAGA GGCGACAGTC GTCGCCGGTC GCCCAGTTCG 
GCCAGCAGCG AAAGTTCTGC CTCTCGCAGC CGATCACCAA CGTCAAGAGA TCTAAAGCAC 
AAGCGCGAAG AGTACATTAA GAAGATCAGT GAGACTAGCC TATTTGCCGA GCTGGTAAAG 
GATCGTCACA AAAGGCAGAA GGCGCTGAAG GAAATCATTG AGCGGCAAGA GGAGAACAGC 
AATAGCAACA GCAACGGTGC ACTGACTATT AACGACAACA GTTCCTCCGT GGATGGCAAT 
ACACCCAACG CGGCTGATGG CCGATCTGCC CCTGGAAGTG GAACACCAGC AGCGGCGTCA 
ACAACTTCCA ATGGGCTTCA AGCTTTGGGT AGCAAGCCGG ATCTGGACCT CAACAACATT 
CCCATGCCAA ACAAGCAGAA CGATTCTGTG GTTTCGAATC CGGCTTCAAA CGCCGATGTT 
CCGGACAGTG TCGCGCAGTT GAAGCAACCT TTGCTGGTGC CACCGTTCTC GGCTAGTAAG 
AACAACATAA AACCGAAGAG CCTGACTTCT TTGCCGCTGC CGCCGGGTAT GAATGTGCTC 
GACTTGGCCG GTGCAAGGTC TCCCTCTCCG GGGCAAAAGA AGGAATCGGA CGAAAAGAAC 
GTTACTTCAA GCGGCAGCGC CAACAAGAGC GTGCTCAATC TTCCTATGCC GCCAGTAATT 
CCCGGCAGCG AAGAGCTTAG TGGCGATGAT GATGTTATCG ACAGTCCTGA AGACTTTGAT 
GCACCCGCGG TTGGCACTGT GCACGGTCAC GGTGGTGGGC CAGGGACAAC TCGTCAGCGC 
CCAGTGATAC TCAACCGTCG TGACTCGCGA AACAACGTAC GCGACTGGGG CGAGCGCTGT 
GTGGATGTTT TTGAGATGAT TGCGCAAATA GGGGAAGGAA CTTACGGACA GGTTTACAAG 
GCGCGGGATC ATCATACCAA TGACATGGTA GCACTGAAGA AGGTTCGGCT GGAGCACGAG 
AAGGAGGGCT TTCCCATTAC AGCTGTTCGT GAAATCAAAA TTCTGAGACA ACTTAACCAT 
CGCAATATAG TCAACCTGCA TGAAATTGTG ACGGACAAGC AGGATGCTGT GGAGTTCCGT 
AAAGACAAGG GATCCTTCTA CTTGGTGTTT GAGTACATGG ATCACGACTT GATGGGTCTG 
CTGGAGTCTG GCATGGTCGA CTTCAACGAG GAAAACAACG CAAGCATTAT GAAGCAGCTC 
TTGGACGGCC TCAACTACTG CCACAAAAAG AATTTTCTGC ATCGAGACAT CAAGTGCTCG 
AACATTTTAA TGAACAACAG GGGTAAAGTA AAACTAGCTG ACTTTGGGCT GGCACGACTA 
TACAATGCGG ACGATCGGGA ACGCCCCTAC ACGAACAAAG TAATTACCTT GTGGTATCGT 
CCTCCTGAGC TTTTGCTGGG TGAGGAGCGT TACGGCCCGT CGATAGACGT GTGGTCCTGC 
GGGTGCATCC TCGGCGAACT CTTTGTGAAG CGGCCCCTGT TCCAGGCTAA TGCGGAAATG 
GCGCAGCTTG AAACGATATC AAAGATCTGT GGATCCCCGG TTCCTGCCGT GTGGCCGAAT 
GTAATTAAGC TGCCTCTGTT CCACACTCTC AAACAAAAGA AGACGCACCG CCGCCGTCTA 
CGAGAAGACT TTGAATTCAT GCCAGCCCCT GCACTTGATC TGCTGGACAA AATGCTTGAT 
TTAGATCCGG ACAAGCGCAT CACAGCAGAA GACGCGCTCC GGTCACCGTG GCTAAGGAAA 
ATCAACCCCG ACGAAATGCC AACGCCACAA TTGCCCACAT GGCAGGACTG CCATGAGCTT 
TGGAGTAAGA AGCGCCGTCG GCAGATGCGG GAGCAACAGG AGTCGCTTCC TCCTACGGTG 
ATAGCCTCGA CCAAATACCA ACAGCATGGA GCAACCATGG TGGGGGATGC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649325.2
CDS	396..3869
Translation	MHASSAAATALVEYSDVSSEDFSDQEAGDLDADAGKGAGNIKKPKPAPDNQFSKGRLDAKPDKEGYDNYRSRRAEDSSDPVAAGSRQTSSSEATNPREEPSQASNTSKDELWGREIYMSSDSIDTDELEAEMKRQKRKKQKKEKHKHKSKKKSKKRKKKRAKSYSSIDSMSDNDINALLDRRYTPPTAPSKSNERTVSAAPSSFTPHNLKESSSPATPPPVRRPNTNSNYYGESSLETANSALGSNLQVTVTNKQSISNRLRSPPPSSRSSGNGPRFGNSPRTPPPSHYSSSGGGGVGSGSVVRDSRSSRYVNSPHKEDVSAHHRSSHDHGYQGRYSGAGSSSHDTRKVKRLSPELDRYNHQPSTPPHKRRKFSDGREVGLGNFEHSRHHSGKYERYSRDRYSRRSSRSPSVQHSRSRQSPSGGLSSGSNAFRHGGSHKHKYGTTVSSTPSHTTRTSKRASGTGTSGDRYSRSPRTSSRYMESSPPSPVGASGSHHYHHRRSPRMRQRTRGDSRRRSPSSASSESSASRSRSPTSRDLKHKREEYIKKISETSLFAELVKDRHKRQKALKEIIERQEENSNSNSNGALTINDNSSSVDGNTPNAADGRSAPGSGTPAAASTTSNGLQALGSKPDLDLNNIPMPNKQNDSVVSNPASNADVPDSVAQLKQPLLVPPFSASKNNIKPKSLTSLPLPPGMNVLDLAGARSPSPGQKKESDEKNVTSSGSANKSVLNLPMPPVIPGSEELSGDDDVIDSPEDFDAPAVGTVHGHGGGPGTTRQRPVILNRRDSRNNVRDWGERCVDVFEMIAQIGEGTYGQVYKARDHHTNDMVALKKVRLEHEKEGFPITAVREIKILRQLNHRNIVNLHEIVTDKQDAVEFRKDKGSFYLVFEYMDHDLMGLLESGMVDFNEENNASIMKQLLDGLNYCHKKNFLHRDIKCSNILMNNRGKVKLADFGLARLYNADDRERPYTNKVITLWYRPPELLLGEERYGPSIDVWSCGCILGELFVKRPLFQANAEMAQLETISKICGSPVPAVWPNVIKLPLFHTLKQKKTHRRRLREDFEFMPAPALDLLDKMLDLDPDKRITAEDALRSPWLRKINPDEMPTPQLPTWQDCHELWSKKRRRQMREQQESLPPTVIASTKYQQHGATMVGDA

Target ORF information:

RefSeq Version	NM_141068.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Cyclin-dependent kinase 12 (Cdk12), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141068.4

ORF Insert Sequence:

ATGCATGCAT CCTCCGCGGC GGCCACTGCA CTTGTGGAGT ACTCGGACGT CAGCTCAGAG 
GACTTCTCGG ATCAGGAGGC AGGGGACTTG GATGCGGACG CCGGCAAGGG TGCCGGAAAC 
ATCAAAAAAC CGAAGCCAGC TCCGGATAAT CAGTTCTCCA AAGGTCGTCT TGATGCCAAG 
CCCGATAAAG AGGGTTATGA TAACTACAGA AGTAGAAGGG CGGAAGACTC AAGCGACCCA 
GTGGCGGCCG GATCGAGGCA AACGTCTAGC AGCGAGGCCA CGAATCCGCG GGAGGAACCT 
TCGCAGGCAT CCAACACCTC GAAGGACGAG CTGTGGGGCA GAGAGATATA CATGTCCAGC 
GATTCTATCG ACACCGATGA GCTGGAGGCG GAAATGAAGC GGCAGAAGCG CAAAAAGCAG 
AAAAAGGAAA AACACAAACA CAAGTCGAAG AAAAAGTCCA AGAAGCGCAA GAAGAAGCGG 
GCCAAGTCAT ACTCCAGCAT CGATTCGATG TCAGACAATG ACATCAATGC CTTACTGGAC 
CGGCGCTATA CTCCGCCGAC GGCTCCTAGC AAGAGCAACG AACGGACGGT TAGCGCTGCG 
CCGTCCTCCT TCACCCCACA CAACCTCAAG GAGAGCAGTT CGCCAGCCAC TCCACCGCCA 
GTGCGTCGCC CGAATACGAA CAGCAACTAC TACGGCGAGT CGTCCCTGGA GACGGCCAAC 
TCTGCACTGG GAAGCAACCT GCAGGTCACA GTTACGAATA AGCAGTCGAT CAGCAACCGG 
CTTCGTTCTC CTCCTCCGTC CTCCCGCTCC AGCGGCAACG GGCCGAGGTT TGGAAACTCC 
CCGCGAACTC CACCGCCCTC TCACTACAGC TCCAGTGGCG GAGGAGGGGT TGGCAGTGGG 
TCAGTGGTCC GCGATTCACG CAGCTCCCGC TATGTAAATA GTCCCCACAA AGAGGATGTA 
AGTGCGCATC ACCGCTCTAG CCACGATCAC GGATATCAGG GACGTTACTC TGGTGCTGGG 
TCGAGCAGTC ACGATACCAG GAAGGTGAAA CGACTGTCGC CGGAGCTGGA CCGCTACAAC 
CATCAGCCAA GCACGCCACC CCACAAGCGT CGGAAGTTTA GCGATGGCAG GGAAGTGGGC 
CTCGGAAACT TTGAGCACAG TAGACACCAC TCTGGAAAGT ACGAACGATA CAGCAGAGAT 
CGATATTCTA GGCGGTCATC AAGGAGCCCC AGTGTGCAGC ACTCACGAAG TCGGCAATCT 
CCCAGCGGCG GCCTGTCAAG TGGTTCGAAC GCGTTTCGCC ATGGTGGCAG TCACAAACAC 
AAGTATGGGA CCACTGTTAG CTCGACGCCG TCGCATACGA CGAGGACCTC AAAACGCGCT 
TCGGGAACTG GTACCTCTGG CGATCGCTAC TCGAGGTCGC CCAGAACAAG TTCGCGGTAC 
ATGGAGTCAT CACCGCCATC GCCAGTCGGA GCTTCCGGTT CGCATCACTA TCATCATCGC 
CGTTCGCCAA GGATGCGCCA GAGGACCAGA GGCGACAGTC GTCGCCGGTC GCCCAGTTCG 
GCCAGCAGCG AAAGTTCTGC CTCTCGCAGC CGATCACCAA CGTCAAGAGA TCTAAAGCAC 
AAGCGCGAAG AGTACATTAA GAAGATCAGT GAGACTAGCC TATTTGCCGA GCTGGTAAAG 
GATCGTCACA AAAGGCAGAA GGCGCTGAAG GAAATCATTG AGCGGCAAGA GGAGAACAGC 
AATAGCAACA GCAACGGTGC ACTGACTATT AACGACAACA GTTCCTCCGT GGATGGCAAT 
ACACCCAACG CGGCTGATGG CCGATCTGCC CCTGGAAGTG GAACACCAGC AGCGGCGTCA 
ACAACTTCCA ATGGGCTTCA AGCTTTGGGT AGCAAGCCGG ATCTGGACCT CAACAACATT 
CCCATGCCAA ACAAGCAGAA CGATTCTGTG GTTTCGAATC CGGCTTCAAA CGCCGATGTT 
CCGGACAGTG TCGCGCAGTT GAAGCAACCT TTGCTGGTGC CACCGTTCTC GGCTAGTAAG 
AACAACATAA AACCGAAGAG CCTGACTTCT TTGCCGCTGC CGCCGGGTAT GAATGTGCTC 
GACTTGGCCG GTGCAAGGTC TCCCTCTCCG GGGCAAAAGA AGGAATCGGA CGAAAAGAAC 
GTTACTTCAA GCGGCAGCGC CAACAAGAGC GTGCTCAATC TTCCTATGCC GCCAGTAATT 
CCCGGCAGCG AAGAGCTTAG TGGCGATGAT GATGTTATCG ACAGTCCTGA AGACTTTGAT 
GCACCCGCGG TTGGCACTGT GCACGGTCAC GGTGGTGGGC CAGGGACAAC TCGTCAGCGC 
CCAGTGATAC TCAACCGTCG TGACTCGCGA AACAACGTAC GCGACTGGGG CGAGCGCTGT 
GTGGATGTTT TTGAGATGAT TGCGCAAATA GGGGAAGGAA CTTACGGACA GGTTTACAAG 
GCGCGGGATC ATCATACCAA TGACATGGTA GCACTGAAGA AGGTTCGGCT GGAGCACGAG 
AAGGAGGGCT TTCCCATTAC AGCTGTTCGT GAAATCAAAA TTCTGAGACA ACTTAACCAT 
CGCAATATAG TCAACCTGCA TGAAATTGTG ACGGACAAGC AGGATGCTGT GGAGTTCCGT 
AAAGACAAGG GATCCTTCTA CTTGGTGTTT GAGTACATGG ATCACGACTT GATGGGTCTG 
CTGGAGTCTG GCATGGTCGA CTTCAACGAG GAAAACAACG CAAGCATTAT GAAGCAGCTC 
TTGGACGGCC TCAACTACTG CCACAAAAAG AATTTTCTGC ATCGAGACAT CAAGTGCTCG 
AACATTTTAA TGAACAACAG GGGTAAAGTA AAACTAGCTG ACTTTGGGCT GGCACGACTA 
TACAATGCGG ACGATCGGGA ACGCCCCTAC ACGAACAAAG TAATTACCTT GTGGTATCGT 
CCTCCTGAGC TTTTGCTGGG TGAGGAGCGT TACGGCCCGT CGATAGACGT GTGGTCCTGC 
GGGTGCATCC TCGGCGAACT CTTTGTGAAG CGGCCCCTGT TCCAGGCTAA TGCGGAAATG 
GCGCAGCTTG AAACGATATC AAAGATCTGT GGATCCCCGG TTCCTGCCGT GTGGCCGAAT 
GTAATTAAGC TGCCTCTGTT CCACACTCTC AAACAAAAGA AGACGCACCG CCGCCGTCTA 
CGAGAAGACT TTGAATTCAT GCCAGCCCCT GCACTTGATC TGCTGGACAA AATGCTTGAT 
TTAGATCCGG ACAAGCGCAT CACAGCAGAA GACGCGCTCC GGTCACCGTG GCTAAGGAAA 
ATCAACCCCG ACGAAATGCC AACGCCACAA TTGCCCACAT GGCAGGACTG CCATGAGCTT 
TGGAGTAAGA AGCGCCGTCG GCAGATGCGG GAGCAACAGG AGTCGCTTCC TCCTACGGTG 
ATAGCCTCGA CCAAATACCA ACAGCATGGA GCAACCATGG TGGGGGATGC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
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Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
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The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
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Combined evidence annotation of transposable elements in genome sequences.
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Cdk12 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

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ORF Insert Sequence:

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ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

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