barc cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	barc
Entrez Gene ID	40369
Full Name	barricade
Synonyms	CG6049,Dmel\CG6049,Tat-SF1,cg6049,cus2
General protein information	Preferred Names barricade Names CG6049 gene product from transcript CG6049-RC CG6049-PA CG6049-PC barc-PA barc-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 78D1-78D2

mRNA and Protein(s)

mRNA	Protein	Name
NM_168902.3	NP_730629.1	barricade, isoform C
NM_141056.4	NP_649313.1	barricade, isoform A

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	barc	NP_730629.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006266	XP_316330.4
Caenorhabditis elegans (roundworm)	Y65B4A.1	NP_490765.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Barc, together with the U2 snRNP complex, plays an important role in regulating neural stem cell lineage progression during brain development and facilitates correct splicing of a subset of introns.
Title: The splicing co-factor Barricade/Tat-SF1 is required for cell cycle and lineage progression in

The following barc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the barc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa20316	NM_141056.4 Latest version!	Drosophila melanogaster barricade (barc), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OFa20316	NM_168902.3 Latest version!	Drosophila melanogaster barricade (barc), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

barc ( NM_168902.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730629.1 Drosophila melanogaster barricade (barc), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa20316
Clone ID Related Accession (Same CDS sequence)	NM_168902.3 , NM_141056.4
Accession Version	NM_168902.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	barricade, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168902.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGACG AAGGTGGCTG TAAATCGGAG CAGTTAGAGA AATCCGAAGA AGCGGAGGAA 
AAAAAGGGGG ACGCAGAAGG ACAGGAAGCG AAAGCTCCAA TTCTCAACCC CATTTCAGTC 
CCAGAAGTTG ATGACAAGCC GACGGAAAAT AAACCCCAAT CGGATAATCA CGCGGACAAA 
ACGGACGAAA CTCCATCTCA GGACTTTGCC GCCTACGAGG AGCACATGAC CTATGGAGCG 
GACGGCGGGG CTATATACAC GGATCCCAGC ACGAAGCAGA AATACAAGTG GTGCGCCACC 
GGAAACAATT GGCAACCCTT AGGCGTTGAT GAAGATGTTG ATGGACAGGC TGAAGATCCC 
TACGAAAACG AGCACTACAA GTGGTGTCCC AAATCCCAAC AGTGGTTGCC AAAAAAACAG 
GAAACTGAAA CGGAGCACTA CAAGTGGGAT GACGAGCAAA AGAAGTGGGT GCCCAAGCAC 
CCGAACCCTG GCCAGGAAGG GGTTTGCGGT GTGGATGAGC ATGGCGAGCG CACTTACACC 
GACAAGGATG GCGTCGAGTT CTTTTGGGAT GCAACCAAGA GTGCTTGGTT CCCCAAGATC 
GATGATGATT TCATGGCTCG CTACCAAATG AACTATGGAT TTATTGACAA CACATCAGCG 
GGGGAGAAAG AGAAGGCTGA AAAGGAGGCG GCTGAGGCCA AGAGAAAGGA GGAGGAACTC 
AAGCGTATGA CGGCAGAGGC GGAAGCTGCT ATGTCTAGAG ACAATCCAGC ATCCAGCGCT 
GCAGTGCCCA CTGGCAAGCG AAAGGCCCAG GAGCCCCCAA AATGGTTCGA AATGGATCCA 
TTGCAGAACA CCAAGGTGTA TGTCTCCAAT TTGCCCCTGG ACATTACTAT GGATGAGTTT 
GCCGATCTGA TGGGCAAGTG TGGCATGGTA ATGAGAGATC CGCAGACGCA GAAATTCAAG 
CTGAAACTAT ATGCCGAAAA GGATGGACAA ATCAAGGGCG ATGGCTTGTG TGATTACATC 
AAGGTGGAGA GTGTCAATCT GGCGCTGAAA ATTTTAGACG AGTACAATCT GCGTGGCCAC 
AAGATTCGTG TCCAGAGGGC TCAGTTCCAA ATGCGTGGAG AATACAATCC CGCTCTGAAG 
CCCAAACGCA AGAAGAAGGA CAAAGAGAAA TTGCAAAAGA TGAAGGAAAA GTTATTTGAT 
TGGCGTCCAG ATAAATTGCG TGGCGAACGG TCAAAGAATG AGAAAACCGT CATCATTAAA 
AACCTCTTCA CCCCAGAACT CTTTGAGAAG GAAGTGGAGC TCATATTGGA GTACCAAAAC 
AATCTGCGTG AGGAGTGCAG CAAATGCGGG ATGGTCCGTA AAGTGGTTAT CTATGATCGC 
CATCCTGATG GTGTAGCCCA GATCAACATG GCCTCGCCGG AGGAAGCTGA CCTCGTCATT 
CAAATGATGC AGGGGCGTTA TTTTGGACAG CGGCAACTAA GTGCGGAGGC CTGGGATGGC 
AAGACCAAAT ACAAAATTGA GGAATCAGCT GTCGAGGCGC ATGAACGGCT TTCCAAATGG 
GATGAATTCT TGGCAGAAGA AGAAACCGAT AAAAAAGCTT CCGAGGATAT GAAGGAGGAG 
GACGTTGACT CGCCCGAAAA CCAGCTTTTG CCAGGAGACG CCACCCCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730629.1
CDS	289..1959
Translation	MSDEGGCKSEQLEKSEEAEEKKGDAEGQEAKAPILNPISVPEVDDKPTENKPQSDNHADKTDETPSQDFAAYEEHMTYGADGGAIYTDPSTKQKYKWCATGNNWQPLGVDEDVDGQAEDPYENEHYKWCPKSQQWLPKKQETETEHYKWDDEQKKWVPKHPNPGQEGVCGVDEHGERTYTDKDGVEFFWDATKSAWFPKIDDDFMARYQMNYGFIDNTSAGEKEKAEKEAAEAKRKEEELKRMTAEAEAAMSRDNPASSAAVPTGKRKAQEPPKWFEMDPLQNTKVYVSNLPLDITMDEFADLMGKCGMVMRDPQTQKFKLKLYAEKDGQIKGDGLCDYIKVESVNLALKILDEYNLRGHKIRVQRAQFQMRGEYNPALKPKRKKKDKEKLQKMKEKLFDWRPDKLRGERSKNEKTVIIKNLFTPELFEKEVELILEYQNNLREECSKCGMVRKVVIYDRHPDGVAQINMASPEEADLVIQMMQGRYFGQRQLSAEAWDGKTKYKIEESAVEAHERLSKWDEFLAEEETDKKASEDMKEEDVDSPENQLLPGDATP

Target ORF information:

RefSeq Version	NM_168902.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster barricade (barc), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168902.3

ORF Insert Sequence:

ATGAGCGACG AAGGTGGCTG TAAATCGGAG CAGTTAGAGA AATCCGAAGA AGCGGAGGAA 
AAAAAGGGGG ACGCAGAAGG ACAGGAAGCG AAAGCTCCAA TTCTCAACCC CATTTCAGTC 
CCAGAAGTTG ATGACAAGCC GACGGAAAAT AAACCCCAAT CGGATAATCA CGCGGACAAA 
ACGGACGAAA CTCCATCTCA GGACTTTGCC GCCTACGAGG AGCACATGAC CTATGGAGCG 
GACGGCGGGG CTATATACAC GGATCCCAGC ACGAAGCAGA AATACAAGTG GTGCGCCACC 
GGAAACAATT GGCAACCCTT AGGCGTTGAT GAAGATGTTG ATGGACAGGC TGAAGATCCC 
TACGAAAACG AGCACTACAA GTGGTGTCCC AAATCCCAAC AGTGGTTGCC AAAAAAACAG 
GAAACTGAAA CGGAGCACTA CAAGTGGGAT GACGAGCAAA AGAAGTGGGT GCCCAAGCAC 
CCGAACCCTG GCCAGGAAGG GGTTTGCGGT GTGGATGAGC ATGGCGAGCG CACTTACACC 
GACAAGGATG GCGTCGAGTT CTTTTGGGAT GCAACCAAGA GTGCTTGGTT CCCCAAGATC 
GATGATGATT TCATGGCTCG CTACCAAATG AACTATGGAT TTATTGACAA CACATCAGCG 
GGGGAGAAAG AGAAGGCTGA AAAGGAGGCG GCTGAGGCCA AGAGAAAGGA GGAGGAACTC 
AAGCGTATGA CGGCAGAGGC GGAAGCTGCT ATGTCTAGAG ACAATCCAGC ATCCAGCGCT 
GCAGTGCCCA CTGGCAAGCG AAAGGCCCAG GAGCCCCCAA AATGGTTCGA AATGGATCCA 
TTGCAGAACA CCAAGGTGTA TGTCTCCAAT TTGCCCCTGG ACATTACTAT GGATGAGTTT 
GCCGATCTGA TGGGCAAGTG TGGCATGGTA ATGAGAGATC CGCAGACGCA GAAATTCAAG 
CTGAAACTAT ATGCCGAAAA GGATGGACAA ATCAAGGGCG ATGGCTTGTG TGATTACATC 
AAGGTGGAGA GTGTCAATCT GGCGCTGAAA ATTTTAGACG AGTACAATCT GCGTGGCCAC 
AAGATTCGTG TCCAGAGGGC TCAGTTCCAA ATGCGTGGAG AATACAATCC CGCTCTGAAG 
CCCAAACGCA AGAAGAAGGA CAAAGAGAAA TTGCAAAAGA TGAAGGAAAA GTTATTTGAT 
TGGCGTCCAG ATAAATTGCG TGGCGAACGG TCAAAGAATG AGAAAACCGT CATCATTAAA 
AACCTCTTCA CCCCAGAACT CTTTGAGAAG GAAGTGGAGC TCATATTGGA GTACCAAAAC 
AATCTGCGTG AGGAGTGCAG CAAATGCGGG ATGGTCCGTA AAGTGGTTAT CTATGATCGC 
CATCCTGATG GTGTAGCCCA GATCAACATG GCCTCGCCGG AGGAAGCTGA CCTCGTCATT 
CAAATGATGC AGGGGCGTTA TTTTGGACAG CGGCAACTAA GTGCGGAGGC CTGGGATGGC 
AAGACCAAAT ACAAAATTGA GGAATCAGCT GTCGAGGCGC ATGAACGGCT TTCCAAATGG 
GATGAATTCT TGGCAGAAGA AGAAACCGAT AAAAAAGCTT CCGAGGATAT GAAGGAGGAG 
GACGTTGACT CGCCCGAAAA CCAGCTTTTG CCAGGAGACG CCACCCCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

barc ( NM_141056.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649313.1 Drosophila melanogaster barricade (barc), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa20316
Clone ID Related Accession (Same CDS sequence)	NM_168902.3 , NM_141056.4
Accession Version	NM_141056.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	barricade, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_141056.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGACG AAGGTGGCTG TAAATCGGAG CAGTTAGAGA AATCCGAAGA AGCGGAGGAA 
AAAAAGGGGG ACGCAGAAGG ACAGGAAGCG AAAGCTCCAA TTCTCAACCC CATTTCAGTC 
CCAGAAGTTG ATGACAAGCC GACGGAAAAT AAACCCCAAT CGGATAATCA CGCGGACAAA 
ACGGACGAAA CTCCATCTCA GGACTTTGCC GCCTACGAGG AGCACATGAC CTATGGAGCG 
GACGGCGGGG CTATATACAC GGATCCCAGC ACGAAGCAGA AATACAAGTG GTGCGCCACC 
GGAAACAATT GGCAACCCTT AGGCGTTGAT GAAGATGTTG ATGGACAGGC TGAAGATCCC 
TACGAAAACG AGCACTACAA GTGGTGTCCC AAATCCCAAC AGTGGTTGCC AAAAAAACAG 
GAAACTGAAA CGGAGCACTA CAAGTGGGAT GACGAGCAAA AGAAGTGGGT GCCCAAGCAC 
CCGAACCCTG GCCAGGAAGG GGTTTGCGGT GTGGATGAGC ATGGCGAGCG CACTTACACC 
GACAAGGATG GCGTCGAGTT CTTTTGGGAT GCAACCAAGA GTGCTTGGTT CCCCAAGATC 
GATGATGATT TCATGGCTCG CTACCAAATG AACTATGGAT TTATTGACAA CACATCAGCG 
GGGGAGAAAG AGAAGGCTGA AAAGGAGGCG GCTGAGGCCA AGAGAAAGGA GGAGGAACTC 
AAGCGTATGA CGGCAGAGGC GGAAGCTGCT ATGTCTAGAG ACAATCCAGC ATCCAGCGCT 
GCAGTGCCCA CTGGCAAGCG AAAGGCCCAG GAGCCCCCAA AATGGTTCGA AATGGATCCA 
TTGCAGAACA CCAAGGTGTA TGTCTCCAAT TTGCCCCTGG ACATTACTAT GGATGAGTTT 
GCCGATCTGA TGGGCAAGTG TGGCATGGTA ATGAGAGATC CGCAGACGCA GAAATTCAAG 
CTGAAACTAT ATGCCGAAAA GGATGGACAA ATCAAGGGCG ATGGCTTGTG TGATTACATC 
AAGGTGGAGA GTGTCAATCT GGCGCTGAAA ATTTTAGACG AGTACAATCT GCGTGGCCAC 
AAGATTCGTG TCCAGAGGGC TCAGTTCCAA ATGCGTGGAG AATACAATCC CGCTCTGAAG 
CCCAAACGCA AGAAGAAGGA CAAAGAGAAA TTGCAAAAGA TGAAGGAAAA GTTATTTGAT 
TGGCGTCCAG ATAAATTGCG TGGCGAACGG TCAAAGAATG AGAAAACCGT CATCATTAAA 
AACCTCTTCA CCCCAGAACT CTTTGAGAAG GAAGTGGAGC TCATATTGGA GTACCAAAAC 
AATCTGCGTG AGGAGTGCAG CAAATGCGGG ATGGTCCGTA AAGTGGTTAT CTATGATCGC 
CATCCTGATG GTGTAGCCCA GATCAACATG GCCTCGCCGG AGGAAGCTGA CCTCGTCATT 
CAAATGATGC AGGGGCGTTA TTTTGGACAG CGGCAACTAA GTGCGGAGGC CTGGGATGGC 
AAGACCAAAT ACAAAATTGA GGAATCAGCT GTCGAGGCGC ATGAACGGCT TTCCAAATGG 
GATGAATTCT TGGCAGAAGA AGAAACCGAT AAAAAAGCTT CCGAGGATAT GAAGGAGGAG 
GACGTTGACT CGCCCGAAAA CCAGCTTTTG CCAGGAGACG CCACCCCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649313.1
CDS	304..1974
Translation	MSDEGGCKSEQLEKSEEAEEKKGDAEGQEAKAPILNPISVPEVDDKPTENKPQSDNHADKTDETPSQDFAAYEEHMTYGADGGAIYTDPSTKQKYKWCATGNNWQPLGVDEDVDGQAEDPYENEHYKWCPKSQQWLPKKQETETEHYKWDDEQKKWVPKHPNPGQEGVCGVDEHGERTYTDKDGVEFFWDATKSAWFPKIDDDFMARYQMNYGFIDNTSAGEKEKAEKEAAEAKRKEEELKRMTAEAEAAMSRDNPASSAAVPTGKRKAQEPPKWFEMDPLQNTKVYVSNLPLDITMDEFADLMGKCGMVMRDPQTQKFKLKLYAEKDGQIKGDGLCDYIKVESVNLALKILDEYNLRGHKIRVQRAQFQMRGEYNPALKPKRKKKDKEKLQKMKEKLFDWRPDKLRGERSKNEKTVIIKNLFTPELFEKEVELILEYQNNLREECSKCGMVRKVVIYDRHPDGVAQINMASPEEADLVIQMMQGRYFGQRQLSAEAWDGKTKYKIEESAVEAHERLSKWDEFLAEEETDKKASEDMKEEDVDSPENQLLPGDATP

Target ORF information:

RefSeq Version	NM_141056.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster barricade (barc), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141056.4

ORF Insert Sequence:

ATGAGCGACG AAGGTGGCTG TAAATCGGAG CAGTTAGAGA AATCCGAAGA AGCGGAGGAA 
AAAAAGGGGG ACGCAGAAGG ACAGGAAGCG AAAGCTCCAA TTCTCAACCC CATTTCAGTC 
CCAGAAGTTG ATGACAAGCC GACGGAAAAT AAACCCCAAT CGGATAATCA CGCGGACAAA 
ACGGACGAAA CTCCATCTCA GGACTTTGCC GCCTACGAGG AGCACATGAC CTATGGAGCG 
GACGGCGGGG CTATATACAC GGATCCCAGC ACGAAGCAGA AATACAAGTG GTGCGCCACC 
GGAAACAATT GGCAACCCTT AGGCGTTGAT GAAGATGTTG ATGGACAGGC TGAAGATCCC 
TACGAAAACG AGCACTACAA GTGGTGTCCC AAATCCCAAC AGTGGTTGCC AAAAAAACAG 
GAAACTGAAA CGGAGCACTA CAAGTGGGAT GACGAGCAAA AGAAGTGGGT GCCCAAGCAC 
CCGAACCCTG GCCAGGAAGG GGTTTGCGGT GTGGATGAGC ATGGCGAGCG CACTTACACC 
GACAAGGATG GCGTCGAGTT CTTTTGGGAT GCAACCAAGA GTGCTTGGTT CCCCAAGATC 
GATGATGATT TCATGGCTCG CTACCAAATG AACTATGGAT TTATTGACAA CACATCAGCG 
GGGGAGAAAG AGAAGGCTGA AAAGGAGGCG GCTGAGGCCA AGAGAAAGGA GGAGGAACTC 
AAGCGTATGA CGGCAGAGGC GGAAGCTGCT ATGTCTAGAG ACAATCCAGC ATCCAGCGCT 
GCAGTGCCCA CTGGCAAGCG AAAGGCCCAG GAGCCCCCAA AATGGTTCGA AATGGATCCA 
TTGCAGAACA CCAAGGTGTA TGTCTCCAAT TTGCCCCTGG ACATTACTAT GGATGAGTTT 
GCCGATCTGA TGGGCAAGTG TGGCATGGTA ATGAGAGATC CGCAGACGCA GAAATTCAAG 
CTGAAACTAT ATGCCGAAAA GGATGGACAA ATCAAGGGCG ATGGCTTGTG TGATTACATC 
AAGGTGGAGA GTGTCAATCT GGCGCTGAAA ATTTTAGACG AGTACAATCT GCGTGGCCAC 
AAGATTCGTG TCCAGAGGGC TCAGTTCCAA ATGCGTGGAG AATACAATCC CGCTCTGAAG 
CCCAAACGCA AGAAGAAGGA CAAAGAGAAA TTGCAAAAGA TGAAGGAAAA GTTATTTGAT 
TGGCGTCCAG ATAAATTGCG TGGCGAACGG TCAAAGAATG AGAAAACCGT CATCATTAAA 
AACCTCTTCA CCCCAGAACT CTTTGAGAAG GAAGTGGAGC TCATATTGGA GTACCAAAAC 
AATCTGCGTG AGGAGTGCAG CAAATGCGGG ATGGTCCGTA AAGTGGTTAT CTATGATCGC 
CATCCTGATG GTGTAGCCCA GATCAACATG GCCTCGCCGG AGGAAGCTGA CCTCGTCATT 
CAAATGATGC AGGGGCGTTA TTTTGGACAG CGGCAACTAA GTGCGGAGGC CTGGGATGGC 
AAGACCAAAT ACAAAATTGA GGAATCAGCT GTCGAGGCGC ATGAACGGCT TTCCAAATGG 
GATGAATTCT TGGCAGAAGA AGAAACCGAT AAAAAAGCTT CCGAGGATAT GAAGGAGGAG 
GACGTTGACT CGCCCGAAAA CCAGCTTTTG CCAGGAGACG CCACCCCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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barc cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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