pzg cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	pzg
Entrez Gene ID	40351
Full Name	putzig
Synonyms	CG7752,Dmel\CG7752,Pzg,Pzg/Z4,Q9VP57_DROME,Z4,p160,z4
General protein information	Preferred Names putzig Names CG7752 gene product from transcript CG7752-RA CG7752-PA CG7752-PB Z4 pzg-PA pzg-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 78C5-78C6

mRNA and Protein(s)

mRNA	Protein	Name
NM_141040.2	NP_649297.1	putzig, isoform A
NM_001275214.1	NP_001262143.1	putzig, isoform B

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006534	XP_316571.4
Drosophila melanogaster (fruit fly)	pzg	NP_001262143.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

downregulation of pzg activity in the developing wing imaginal discs induces an apoptotic response.
Title: Loss of putzig Activity Results in Apoptosis during Wing Imaginal Development in Drosophila.

JIL-1 and Z4 (also known as Putzig), which are necessary for establishing a fine-tuned regulation of the transcription of the major component of Drosophila telomeres, the HeT-A retrotransposon, thus guaranteeing genome stability
Title: The chromosomal proteins JIL-1 and Z4/Putzig regulate the telomeric chromatin in Drosophila melanogaster.

Chriz and Z4 form a complex that recruits the JIL-1 kinase to polytene chromosomes, enabling local H3S10 phosphorylation of interband nucleosomal histones.
Title: The Chriz-Z4 complex recruits JIL-1 to polytene chromosomes, a requirement for interband-specific phosphorylation of H3S10.

Pzg is associated with EcR in vivo and required for the transcriptional induction of EcR target genes, whereas reduced ecdysteroid levels imply a NURF-independent function of Pzg.
Title: The Putzig-NURF nucleosome remodeling complex is required for ecdysone receptor signaling and innate immunity in Drosophila melanogaster.

These data suggest a novel Putzig-nuclear remodeling factor chromatin complex required for epigenetic activation of Notch targets.
Title: A novel Pzg-NURF complex regulates Notch target gene activity.

The following pzg gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pzg cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa19190	NM_141040.2 Latest version!	Drosophila melanogaster putzig (pzg), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa19190	NM_001275214.1 Latest version!	Drosophila melanogaster putzig (pzg), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pzg ( NM_141040.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649297.1 Drosophila melanogaster putzig (pzg), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa19190
Clone ID Related Accession (Same CDS sequence)	NM_141040.2 , NM_001275214.1
Accession Version	NM_141040.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2991bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	putzig, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_141040.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACAACC AACTGAATCC GGCCACCTAC CGCAAGTTTA ACAATCTGCT CAGTAGCGGA 
GCGGTCACTG TGACCGGTCC CAGCCAGCCG CGAATCCTCA AGTCGACGCG TATGGTGGTA 
CCCGGTGGTG GCGGAGGAGG AGGTGCCTCT GCCGGAGGAG CCTCAGCCGT TGGCGGCGTA 
GCACAGCAGC AGGCGGTCAA CGATGCAGAG TCGGGTGGCG TGGGTATCGG CGGATTAGCC 
ACGCGATGCT ATATCTGCGA TGACCGCTGC GAGCCACAGA CTTCGCTAAC CGATATGTGC 
ACCACGCACA CTTCCACCAA ATTCCCCAAC AAACTGGCCC AGCTCGTGGG CGAGGGTTTC 
CTCGTGATTG TTTGCGGCGA GGATTACGTG TGCTCACGCT GTACCAACCT GGTGAACTAC 
TACGACCGCC TGGAAAACGA TGTGGAGCGC GTCAAGACGA ACCTCATCAG TTTGCTTAAC 
AAGAAGTATG CCATTAACGA GGATATGGGC CAGGAAGGCA GTCCGCCGCT CAAGATGCAG 
AAAATGGTCG GCGGATCCGC CAATCGCTCG TTGGAGGAGA GTCCTAGTGC GGATTTGCTG 
CGTCCACGTA AACTCCTGCA GGGAAACCCA GTTGGCCAGT CCAAGATCGC CCCAGGAACC 
ACCCAGAGCA GCGTCCAAGG CACCCAGACA GTCCAACGCA AGGCCACCAA GATTTATAAA 
TGCACCTCGT GTGACTATAA AACCTCGGAT ATGCGGCTGT TCAACACGCA TTATGAAACC 
TGCAAGCAGC AGACCTTCCA GTGCAAGACC TGCCGCAAAA TCTTCCCGCA CTTTGGGGCC 
ATGAAGCAGC ACATGGTGCG CGACCACAAT ACCGCCATGG ATAACACGTG TGCCATGTGT 
CACATTAACT TCGTGAACGA GAACAGCCTG CGCAAGCACA TGGAGACGAA TCACGCTACC 
AATGTACTGG TGACCAGCAC CACCACCATA CCGGCATCAG CTGCTCCCGT GGCTGCTGCT 
GCAGCTGCCG CTGCAGCTGC TGCCAACGAG AATCTGGTTG GAACATCGCT ATACACATGC 
AATCATTGCC AGTTCAAGTC AACGGACAAG GTCGTTTTTG ATGAGCATAT GCGCAAGCAT 
GCCGCTGGTA AACCTAAGCC TTTTAAGTGC CGCCTGTGCT CTCAGCGCTT TGAGACGCGC 
GAAGCTGCTA CTGTGCATGC CAAACAGCAT CAGACCAACT TCTTCAAGTG CGGCACTTGT 
TCGATGACCT TCCCGAAACG GGAGATGCTT GTTAAGCACT TCGAAGTGCA TCAGAGTCCT 
TCCGCATCGA CGGTATCGCC CAAGCAGTCA GTTAGCCAGA ACCTGAACAC CCAGAAGCTC 
CTTCAAGAGA CCATTGATGA GGCACTGAGC GATAGCCTGC CTGCTTCGAC AGCTGCTGTG 
GTGGCAACCA CCGAGTCGGA GAACAACATA CGCTTCTTCT CGTGCAGCAT TTGCTCACTG 
ACTTTCATCC AGGAGACATA CTACAACCAC CACATGGAGA CCCACAGACG TGACAAGAAG 
GCAACTTCGG CCGGAGCCAC TGCCCTAAAT TCGGCAGCTA CGGCTTTACT AAGCGATGAG 
CCCGGAGAGG CAACTGGTAA GGCCGGTGAC GAGAGCGGCG AGCAAAACGC AGAGGCCGAC 
ATTGAGAGCC TGTTTGAAAA GCTCCACTCC GACAAGAACG AGAGCGGTGA GGCGCCCAAA 
CCGGGAAGCA AAGGCGGCGA GATGGTTATT ACCTCGCAGG AGGGCAGTGG AGGCATAACC 
TTCAACATTA CTATCCCGCA ACCGGACGGA GACCAATCGC AAAAGGATTC GCCCAATACC 
ACAGCACCTG TCTCTGTTAG CATTGACATG CCAAATTTGG ATCAAGCCGA AGACGAATCA 
CAGTCCCAGG GCAGCATCGA TGCCAAGGGC GAAAGCGGCG AGGCCTCCTC TGCCGATAGC 
AAATCCAATG CCGCACCGGT TTCCATGCCC AGTTTAGATG ATGACAACGA GGCTGCAGCT 
GCTGAAGCTG AGGCCTCAGA GGAAACCAAG ACGGCTAGCA AAGCAGGTCA AGAGAAAGCC 
AAGGCCAAGG CTGGAGAAAG CGCCAAGTCA AAGGAAGCCG CCGCTGCTGA GGAACCACAA 
CCATCTGACG AAGGAGACGT AGTTGCTAAG CGTGAGGCTA CAGATACGCC CACAACTGAG 
TCGGAGGCCA ATGCCACGAC CGCTGAAGCC GGTGAATCTG GCGAAGGCGA GGTTACTGCC 
AGTACTGGAG CCGAGGGCGA GGCGGGTGAA GCTGCAGGTG GCGAGCATGT TGCCATGGAA 
CTTGACGAAG CCATGCAGGC ACAGGTGGAA GGTGGCCAGA TCAAGTTCAT TGTTAACGAG 
AACGGCCATC TCCTCCAGCT GGACAACCAC ATCCTGACGG ACGCCGAAGG CAACCAAATA 
ATTGTGCAAG ATCCCGAGCA AATACAGCAG CTGCTCCAAA GTGTAGGAGT TCTGCAGTCC 
GGCGAGGGAC TTGAGGGCGA AACCCTCCAG ATGATGACCG ATGGCAGTGG CCAGATGGTA 
CTTGTCCATG GTGACAACAA TGAACAGCAG CTTATCGATG CCTCCCTGTT GAACTCCGAG 
GGGCAGTTAA TTATCCAGCA GGGACAGGAT GGTGAGGAAG GCGCTCATGT CATTAGCGAG 
GATGGTACCC GCATCCCAGT GTCCGTGTCG TACACGGAAG ATGGCCAGCC CATCGTCCAG 
GTTCAGCAAC AGGTCCTGGA GGCCGCACAG CAGGCAGCCA GCGCTTCCGG AGGTGCAGAT 
GACAAGGAGG CGGCTGCCGA CTCGGCCGGC GAGGAAGTGC AAGTGTCGGA GGCATCTAGT 
GGCACGGCAT CCACCACCGT TGTTGCCACG AGCACGGCCA GCAGCACTGG TGGCCCCTCC 
GGCGGCAGCT TTTTTGCCCT GGAGGAGTTT ACGGAGACAA AGACCGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649297.1
CDS	339..3329
Translation	MNNQLNPATYRKFNNLLSSGAVTVTGPSQPRILKSTRMVVPGGGGGGGASAGGASAVGGVAQQQAVNDAESGGVGIGGLATRCYICDDRCEPQTSLTDMCTTHTSTKFPNKLAQLVGEGFLVIVCGEDYVCSRCTNLVNYYDRLENDVERVKTNLISLLNKKYAINEDMGQEGSPPLKMQKMVGGSANRSLEESPSADLLRPRKLLQGNPVGQSKIAPGTTQSSVQGTQTVQRKATKIYKCTSCDYKTSDMRLFNTHYETCKQQTFQCKTCRKIFPHFGAMKQHMVRDHNTAMDNTCAMCHINFVNENSLRKHMETNHATNVLVTSTTTIPASAAPVAAAAAAAAAAANENLVGTSLYTCNHCQFKSTDKVVFDEHMRKHAAGKPKPFKCRLCSQRFETREAATVHAKQHQTNFFKCGTCSMTFPKREMLVKHFEVHQSPSASTVSPKQSVSQNLNTQKLLQETIDEALSDSLPASTAAVVATTESENNIRFFSCSICSLTFIQETYYNHHMETHRRDKKATSAGATALNSAATALLSDEPGEATGKAGDESGEQNAEADIESLFEKLHSDKNESGEAPKPGSKGGEMVITSQEGSGGITFNITIPQPDGDQSQKDSPNTTAPVSVSIDMPNLDQAEDESQSQGSIDAKGESGEASSADSKSNAAPVSMPSLDDDNEAAAAEAEASEETKTASKAGQEKAKAKAGESAKSKEAAAAEEPQPSDEGDVVAKREATDTPTTESEANATTAEAGESGEGEVTASTGAEGEAGEAAGGEHVAMELDEAMQAQVEGGQIKFIVNENGHLLQLDNHILTDAEGNQIIVQDPEQIQQLLQSVGVLQSGEGLEGETLQMMTDGSGQMVLVHGDNNEQQLIDASLLNSEGQLIIQQGQDGEEGAHVISEDGTRIPVSVSYTEDGQPIVQVQQQVLEAAQQAASASGGADDKEAAADSAGEEVQVSEASSGTASTTVVATSTASSTGGPSGGSFFALEEFTETKTD

Target ORF information:

RefSeq Version	NM_141040.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster putzig (pzg), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141040.2

ORF Insert Sequence:

ATGAACAACC AACTGAATCC GGCCACCTAC CGCAAGTTTA ACAATCTGCT CAGTAGCGGA 
GCGGTCACTG TGACCGGTCC CAGCCAGCCG CGAATCCTCA AGTCGACGCG TATGGTGGTA 
CCCGGTGGTG GCGGAGGAGG AGGTGCCTCT GCCGGAGGAG CCTCAGCCGT TGGCGGCGTA 
GCACAGCAGC AGGCGGTCAA CGATGCAGAG TCGGGTGGCG TGGGTATCGG CGGATTAGCC 
ACGCGATGCT ATATCTGCGA TGACCGCTGC GAGCCACAGA CTTCGCTAAC CGATATGTGC 
ACCACGCACA CTTCCACCAA ATTCCCCAAC AAACTGGCCC AGCTCGTGGG CGAGGGTTTC 
CTCGTGATTG TTTGCGGCGA GGATTACGTG TGCTCACGCT GTACCAACCT GGTGAACTAC 
TACGACCGCC TGGAAAACGA TGTGGAGCGC GTCAAGACGA ACCTCATCAG TTTGCTTAAC 
AAGAAGTATG CCATTAACGA GGATATGGGC CAGGAAGGCA GTCCGCCGCT CAAGATGCAG 
AAAATGGTCG GCGGATCCGC CAATCGCTCG TTGGAGGAGA GTCCTAGTGC GGATTTGCTG 
CGTCCACGTA AACTCCTGCA GGGAAACCCA GTTGGCCAGT CCAAGATCGC CCCAGGAACC 
ACCCAGAGCA GCGTCCAAGG CACCCAGACA GTCCAACGCA AGGCCACCAA GATTTATAAA 
TGCACCTCGT GTGACTATAA AACCTCGGAT ATGCGGCTGT TCAACACGCA TTATGAAACC 
TGCAAGCAGC AGACCTTCCA GTGCAAGACC TGCCGCAAAA TCTTCCCGCA CTTTGGGGCC 
ATGAAGCAGC ACATGGTGCG CGACCACAAT ACCGCCATGG ATAACACGTG TGCCATGTGT 
CACATTAACT TCGTGAACGA GAACAGCCTG CGCAAGCACA TGGAGACGAA TCACGCTACC 
AATGTACTGG TGACCAGCAC CACCACCATA CCGGCATCAG CTGCTCCCGT GGCTGCTGCT 
GCAGCTGCCG CTGCAGCTGC TGCCAACGAG AATCTGGTTG GAACATCGCT ATACACATGC 
AATCATTGCC AGTTCAAGTC AACGGACAAG GTCGTTTTTG ATGAGCATAT GCGCAAGCAT 
GCCGCTGGTA AACCTAAGCC TTTTAAGTGC CGCCTGTGCT CTCAGCGCTT TGAGACGCGC 
GAAGCTGCTA CTGTGCATGC CAAACAGCAT CAGACCAACT TCTTCAAGTG CGGCACTTGT 
TCGATGACCT TCCCGAAACG GGAGATGCTT GTTAAGCACT TCGAAGTGCA TCAGAGTCCT 
TCCGCATCGA CGGTATCGCC CAAGCAGTCA GTTAGCCAGA ACCTGAACAC CCAGAAGCTC 
CTTCAAGAGA CCATTGATGA GGCACTGAGC GATAGCCTGC CTGCTTCGAC AGCTGCTGTG 
GTGGCAACCA CCGAGTCGGA GAACAACATA CGCTTCTTCT CGTGCAGCAT TTGCTCACTG 
ACTTTCATCC AGGAGACATA CTACAACCAC CACATGGAGA CCCACAGACG TGACAAGAAG 
GCAACTTCGG CCGGAGCCAC TGCCCTAAAT TCGGCAGCTA CGGCTTTACT AAGCGATGAG 
CCCGGAGAGG CAACTGGTAA GGCCGGTGAC GAGAGCGGCG AGCAAAACGC AGAGGCCGAC 
ATTGAGAGCC TGTTTGAAAA GCTCCACTCC GACAAGAACG AGAGCGGTGA GGCGCCCAAA 
CCGGGAAGCA AAGGCGGCGA GATGGTTATT ACCTCGCAGG AGGGCAGTGG AGGCATAACC 
TTCAACATTA CTATCCCGCA ACCGGACGGA GACCAATCGC AAAAGGATTC GCCCAATACC 
ACAGCACCTG TCTCTGTTAG CATTGACATG CCAAATTTGG ATCAAGCCGA AGACGAATCA 
CAGTCCCAGG GCAGCATCGA TGCCAAGGGC GAAAGCGGCG AGGCCTCCTC TGCCGATAGC 
AAATCCAATG CCGCACCGGT TTCCATGCCC AGTTTAGATG ATGACAACGA GGCTGCAGCT 
GCTGAAGCTG AGGCCTCAGA GGAAACCAAG ACGGCTAGCA AAGCAGGTCA AGAGAAAGCC 
AAGGCCAAGG CTGGAGAAAG CGCCAAGTCA AAGGAAGCCG CCGCTGCTGA GGAACCACAA 
CCATCTGACG AAGGAGACGT AGTTGCTAAG CGTGAGGCTA CAGATACGCC CACAACTGAG 
TCGGAGGCCA ATGCCACGAC CGCTGAAGCC GGTGAATCTG GCGAAGGCGA GGTTACTGCC 
AGTACTGGAG CCGAGGGCGA GGCGGGTGAA GCTGCAGGTG GCGAGCATGT TGCCATGGAA 
CTTGACGAAG CCATGCAGGC ACAGGTGGAA GGTGGCCAGA TCAAGTTCAT TGTTAACGAG 
AACGGCCATC TCCTCCAGCT GGACAACCAC ATCCTGACGG ACGCCGAAGG CAACCAAATA 
ATTGTGCAAG ATCCCGAGCA AATACAGCAG CTGCTCCAAA GTGTAGGAGT TCTGCAGTCC 
GGCGAGGGAC TTGAGGGCGA AACCCTCCAG ATGATGACCG ATGGCAGTGG CCAGATGGTA 
CTTGTCCATG GTGACAACAA TGAACAGCAG CTTATCGATG CCTCCCTGTT GAACTCCGAG 
GGGCAGTTAA TTATCCAGCA GGGACAGGAT GGTGAGGAAG GCGCTCATGT CATTAGCGAG 
GATGGTACCC GCATCCCAGT GTCCGTGTCG TACACGGAAG ATGGCCAGCC CATCGTCCAG 
GTTCAGCAAC AGGTCCTGGA GGCCGCACAG CAGGCAGCCA GCGCTTCCGG AGGTGCAGAT 
GACAAGGAGG CGGCTGCCGA CTCGGCCGGC GAGGAAGTGC AAGTGTCGGA GGCATCTAGT 
GGCACGGCAT CCACCACCGT TGTTGCCACG AGCACGGCCA GCAGCACTGG TGGCCCCTCC 
GGCGGCAGCT TTTTTGCCCT GGAGGAGTTT ACGGAGACAA AGACCGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pzg ( NM_001275214.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262143.1 Drosophila melanogaster putzig (pzg), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa19190
Clone ID Related Accession (Same CDS sequence)	NM_141040.2 , NM_001275214.1
Accession Version	NM_001275214.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2991bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	putzig, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACAACC AACTGAATCC GGCCACCTAC CGCAAGTTTA ACAATCTGCT CAGTAGCGGA 
GCGGTCACTG TGACCGGTCC CAGCCAGCCG CGAATCCTCA AGTCGACGCG TATGGTGGTA 
CCCGGTGGTG GCGGAGGAGG AGGTGCCTCT GCCGGAGGAG CCTCAGCCGT TGGCGGCGTA 
GCACAGCAGC AGGCGGTCAA CGATGCAGAG TCGGGTGGCG TGGGTATCGG CGGATTAGCC 
ACGCGATGCT ATATCTGCGA TGACCGCTGC GAGCCACAGA CTTCGCTAAC CGATATGTGC 
ACCACGCACA CTTCCACCAA ATTCCCCAAC AAACTGGCCC AGCTCGTGGG CGAGGGTTTC 
CTCGTGATTG TTTGCGGCGA GGATTACGTG TGCTCACGCT GTACCAACCT GGTGAACTAC 
TACGACCGCC TGGAAAACGA TGTGGAGCGC GTCAAGACGA ACCTCATCAG TTTGCTTAAC 
AAGAAGTATG CCATTAACGA GGATATGGGC CAGGAAGGCA GTCCGCCGCT CAAGATGCAG 
AAAATGGTCG GCGGATCCGC CAATCGCTCG TTGGAGGAGA GTCCTAGTGC GGATTTGCTG 
CGTCCACGTA AACTCCTGCA GGGAAACCCA GTTGGCCAGT CCAAGATCGC CCCAGGAACC 
ACCCAGAGCA GCGTCCAAGG CACCCAGACA GTCCAACGCA AGGCCACCAA GATTTATAAA 
TGCACCTCGT GTGACTATAA AACCTCGGAT ATGCGGCTGT TCAACACGCA TTATGAAACC 
TGCAAGCAGC AGACCTTCCA GTGCAAGACC TGCCGCAAAA TCTTCCCGCA CTTTGGGGCC 
ATGAAGCAGC ACATGGTGCG CGACCACAAT ACCGCCATGG ATAACACGTG TGCCATGTGT 
CACATTAACT TCGTGAACGA GAACAGCCTG CGCAAGCACA TGGAGACGAA TCACGCTACC 
AATGTACTGG TGACCAGCAC CACCACCATA CCGGCATCAG CTGCTCCCGT GGCTGCTGCT 
GCAGCTGCCG CTGCAGCTGC TGCCAACGAG AATCTGGTTG GAACATCGCT ATACACATGC 
AATCATTGCC AGTTCAAGTC AACGGACAAG GTCGTTTTTG ATGAGCATAT GCGCAAGCAT 
GCCGCTGGTA AACCTAAGCC TTTTAAGTGC CGCCTGTGCT CTCAGCGCTT TGAGACGCGC 
GAAGCTGCTA CTGTGCATGC CAAACAGCAT CAGACCAACT TCTTCAAGTG CGGCACTTGT 
TCGATGACCT TCCCGAAACG GGAGATGCTT GTTAAGCACT TCGAAGTGCA TCAGAGTCCT 
TCCGCATCGA CGGTATCGCC CAAGCAGTCA GTTAGCCAGA ACCTGAACAC CCAGAAGCTC 
CTTCAAGAGA CCATTGATGA GGCACTGAGC GATAGCCTGC CTGCTTCGAC AGCTGCTGTG 
GTGGCAACCA CCGAGTCGGA GAACAACATA CGCTTCTTCT CGTGCAGCAT TTGCTCACTG 
ACTTTCATCC AGGAGACATA CTACAACCAC CACATGGAGA CCCACAGACG TGACAAGAAG 
GCAACTTCGG CCGGAGCCAC TGCCCTAAAT TCGGCAGCTA CGGCTTTACT AAGCGATGAG 
CCCGGAGAGG CAACTGGTAA GGCCGGTGAC GAGAGCGGCG AGCAAAACGC AGAGGCCGAC 
ATTGAGAGCC TGTTTGAAAA GCTCCACTCC GACAAGAACG AGAGCGGTGA GGCGCCCAAA 
CCGGGAAGCA AAGGCGGCGA GATGGTTATT ACCTCGCAGG AGGGCAGTGG AGGCATAACC 
TTCAACATTA CTATCCCGCA ACCGGACGGA GACCAATCGC AAAAGGATTC GCCCAATACC 
ACAGCACCTG TCTCTGTTAG CATTGACATG CCAAATTTGG ATCAAGCCGA AGACGAATCA 
CAGTCCCAGG GCAGCATCGA TGCCAAGGGC GAAAGCGGCG AGGCCTCCTC TGCCGATAGC 
AAATCCAATG CCGCACCGGT TTCCATGCCC AGTTTAGATG ATGACAACGA GGCTGCAGCT 
GCTGAAGCTG AGGCCTCAGA GGAAACCAAG ACGGCTAGCA AAGCAGGTCA AGAGAAAGCC 
AAGGCCAAGG CTGGAGAAAG CGCCAAGTCA AAGGAAGCCG CCGCTGCTGA GGAACCACAA 
CCATCTGACG AAGGAGACGT AGTTGCTAAG CGTGAGGCTA CAGATACGCC CACAACTGAG 
TCGGAGGCCA ATGCCACGAC CGCTGAAGCC GGTGAATCTG GCGAAGGCGA GGTTACTGCC 
AGTACTGGAG CCGAGGGCGA GGCGGGTGAA GCTGCAGGTG GCGAGCATGT TGCCATGGAA 
CTTGACGAAG CCATGCAGGC ACAGGTGGAA GGTGGCCAGA TCAAGTTCAT TGTTAACGAG 
AACGGCCATC TCCTCCAGCT GGACAACCAC ATCCTGACGG ACGCCGAAGG CAACCAAATA 
ATTGTGCAAG ATCCCGAGCA AATACAGCAG CTGCTCCAAA GTGTAGGAGT TCTGCAGTCC 
GGCGAGGGAC TTGAGGGCGA AACCCTCCAG ATGATGACCG ATGGCAGTGG CCAGATGGTA 
CTTGTCCATG GTGACAACAA TGAACAGCAG CTTATCGATG CCTCCCTGTT GAACTCCGAG 
GGGCAGTTAA TTATCCAGCA GGGACAGGAT GGTGAGGAAG GCGCTCATGT CATTAGCGAG 
GATGGTACCC GCATCCCAGT GTCCGTGTCG TACACGGAAG ATGGCCAGCC CATCGTCCAG 
GTTCAGCAAC AGGTCCTGGA GGCCGCACAG CAGGCAGCCA GCGCTTCCGG AGGTGCAGAT 
GACAAGGAGG CGGCTGCCGA CTCGGCCGGC GAGGAAGTGC AAGTGTCGGA GGCATCTAGT 
GGCACGGCAT CCACCACCGT TGTTGCCACG AGCACGGCCA GCAGCACTGG TGGCCCCTCC 
GGCGGCAGCT TTTTTGCCCT GGAGGAGTTT ACGGAGACAA AGACCGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262143.1
CDS	339..3329
Translation	MNNQLNPATYRKFNNLLSSGAVTVTGPSQPRILKSTRMVVPGGGGGGGASAGGASAVGGVAQQQAVNDAESGGVGIGGLATRCYICDDRCEPQTSLTDMCTTHTSTKFPNKLAQLVGEGFLVIVCGEDYVCSRCTNLVNYYDRLENDVERVKTNLISLLNKKYAINEDMGQEGSPPLKMQKMVGGSANRSLEESPSADLLRPRKLLQGNPVGQSKIAPGTTQSSVQGTQTVQRKATKIYKCTSCDYKTSDMRLFNTHYETCKQQTFQCKTCRKIFPHFGAMKQHMVRDHNTAMDNTCAMCHINFVNENSLRKHMETNHATNVLVTSTTTIPASAAPVAAAAAAAAAAANENLVGTSLYTCNHCQFKSTDKVVFDEHMRKHAAGKPKPFKCRLCSQRFETREAATVHAKQHQTNFFKCGTCSMTFPKREMLVKHFEVHQSPSASTVSPKQSVSQNLNTQKLLQETIDEALSDSLPASTAAVVATTESENNIRFFSCSICSLTFIQETYYNHHMETHRRDKKATSAGATALNSAATALLSDEPGEATGKAGDESGEQNAEADIESLFEKLHSDKNESGEAPKPGSKGGEMVITSQEGSGGITFNITIPQPDGDQSQKDSPNTTAPVSVSIDMPNLDQAEDESQSQGSIDAKGESGEASSADSKSNAAPVSMPSLDDDNEAAAAEAEASEETKTASKAGQEKAKAKAGESAKSKEAAAAEEPQPSDEGDVVAKREATDTPTTESEANATTAEAGESGEGEVTASTGAEGEAGEAAGGEHVAMELDEAMQAQVEGGQIKFIVNENGHLLQLDNHILTDAEGNQIIVQDPEQIQQLLQSVGVLQSGEGLEGETLQMMTDGSGQMVLVHGDNNEQQLIDASLLNSEGQLIIQQGQDGEEGAHVISEDGTRIPVSVSYTEDGQPIVQVQQQVLEAAQQAASASGGADDKEAAADSAGEEVQVSEASSGTASTTVVATSTASSTGGPSGGSFFALEEFTETKTD

Target ORF information:

RefSeq Version	NM_001275214.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster putzig (pzg), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275214.1

ORF Insert Sequence:

ATGAACAACC AACTGAATCC GGCCACCTAC CGCAAGTTTA ACAATCTGCT CAGTAGCGGA 
GCGGTCACTG TGACCGGTCC CAGCCAGCCG CGAATCCTCA AGTCGACGCG TATGGTGGTA 
CCCGGTGGTG GCGGAGGAGG AGGTGCCTCT GCCGGAGGAG CCTCAGCCGT TGGCGGCGTA 
GCACAGCAGC AGGCGGTCAA CGATGCAGAG TCGGGTGGCG TGGGTATCGG CGGATTAGCC 
ACGCGATGCT ATATCTGCGA TGACCGCTGC GAGCCACAGA CTTCGCTAAC CGATATGTGC 
ACCACGCACA CTTCCACCAA ATTCCCCAAC AAACTGGCCC AGCTCGTGGG CGAGGGTTTC 
CTCGTGATTG TTTGCGGCGA GGATTACGTG TGCTCACGCT GTACCAACCT GGTGAACTAC 
TACGACCGCC TGGAAAACGA TGTGGAGCGC GTCAAGACGA ACCTCATCAG TTTGCTTAAC 
AAGAAGTATG CCATTAACGA GGATATGGGC CAGGAAGGCA GTCCGCCGCT CAAGATGCAG 
AAAATGGTCG GCGGATCCGC CAATCGCTCG TTGGAGGAGA GTCCTAGTGC GGATTTGCTG 
CGTCCACGTA AACTCCTGCA GGGAAACCCA GTTGGCCAGT CCAAGATCGC CCCAGGAACC 
ACCCAGAGCA GCGTCCAAGG CACCCAGACA GTCCAACGCA AGGCCACCAA GATTTATAAA 
TGCACCTCGT GTGACTATAA AACCTCGGAT ATGCGGCTGT TCAACACGCA TTATGAAACC 
TGCAAGCAGC AGACCTTCCA GTGCAAGACC TGCCGCAAAA TCTTCCCGCA CTTTGGGGCC 
ATGAAGCAGC ACATGGTGCG CGACCACAAT ACCGCCATGG ATAACACGTG TGCCATGTGT 
CACATTAACT TCGTGAACGA GAACAGCCTG CGCAAGCACA TGGAGACGAA TCACGCTACC 
AATGTACTGG TGACCAGCAC CACCACCATA CCGGCATCAG CTGCTCCCGT GGCTGCTGCT 
GCAGCTGCCG CTGCAGCTGC TGCCAACGAG AATCTGGTTG GAACATCGCT ATACACATGC 
AATCATTGCC AGTTCAAGTC AACGGACAAG GTCGTTTTTG ATGAGCATAT GCGCAAGCAT 
GCCGCTGGTA AACCTAAGCC TTTTAAGTGC CGCCTGTGCT CTCAGCGCTT TGAGACGCGC 
GAAGCTGCTA CTGTGCATGC CAAACAGCAT CAGACCAACT TCTTCAAGTG CGGCACTTGT 
TCGATGACCT TCCCGAAACG GGAGATGCTT GTTAAGCACT TCGAAGTGCA TCAGAGTCCT 
TCCGCATCGA CGGTATCGCC CAAGCAGTCA GTTAGCCAGA ACCTGAACAC CCAGAAGCTC 
CTTCAAGAGA CCATTGATGA GGCACTGAGC GATAGCCTGC CTGCTTCGAC AGCTGCTGTG 
GTGGCAACCA CCGAGTCGGA GAACAACATA CGCTTCTTCT CGTGCAGCAT TTGCTCACTG 
ACTTTCATCC AGGAGACATA CTACAACCAC CACATGGAGA CCCACAGACG TGACAAGAAG 
GCAACTTCGG CCGGAGCCAC TGCCCTAAAT TCGGCAGCTA CGGCTTTACT AAGCGATGAG 
CCCGGAGAGG CAACTGGTAA GGCCGGTGAC GAGAGCGGCG AGCAAAACGC AGAGGCCGAC 
ATTGAGAGCC TGTTTGAAAA GCTCCACTCC GACAAGAACG AGAGCGGTGA GGCGCCCAAA 
CCGGGAAGCA AAGGCGGCGA GATGGTTATT ACCTCGCAGG AGGGCAGTGG AGGCATAACC 
TTCAACATTA CTATCCCGCA ACCGGACGGA GACCAATCGC AAAAGGATTC GCCCAATACC 
ACAGCACCTG TCTCTGTTAG CATTGACATG CCAAATTTGG ATCAAGCCGA AGACGAATCA 
CAGTCCCAGG GCAGCATCGA TGCCAAGGGC GAAAGCGGCG AGGCCTCCTC TGCCGATAGC 
AAATCCAATG CCGCACCGGT TTCCATGCCC AGTTTAGATG ATGACAACGA GGCTGCAGCT 
GCTGAAGCTG AGGCCTCAGA GGAAACCAAG ACGGCTAGCA AAGCAGGTCA AGAGAAAGCC 
AAGGCCAAGG CTGGAGAAAG CGCCAAGTCA AAGGAAGCCG CCGCTGCTGA GGAACCACAA 
CCATCTGACG AAGGAGACGT AGTTGCTAAG CGTGAGGCTA CAGATACGCC CACAACTGAG 
TCGGAGGCCA ATGCCACGAC CGCTGAAGCC GGTGAATCTG GCGAAGGCGA GGTTACTGCC 
AGTACTGGAG CCGAGGGCGA GGCGGGTGAA GCTGCAGGTG GCGAGCATGT TGCCATGGAA 
CTTGACGAAG CCATGCAGGC ACAGGTGGAA GGTGGCCAGA TCAAGTTCAT TGTTAACGAG 
AACGGCCATC TCCTCCAGCT GGACAACCAC ATCCTGACGG ACGCCGAAGG CAACCAAATA 
ATTGTGCAAG ATCCCGAGCA AATACAGCAG CTGCTCCAAA GTGTAGGAGT TCTGCAGTCC 
GGCGAGGGAC TTGAGGGCGA AACCCTCCAG ATGATGACCG ATGGCAGTGG CCAGATGGTA 
CTTGTCCATG GTGACAACAA TGAACAGCAG CTTATCGATG CCTCCCTGTT GAACTCCGAG 
GGGCAGTTAA TTATCCAGCA GGGACAGGAT GGTGAGGAAG GCGCTCATGT CATTAGCGAG 
GATGGTACCC GCATCCCAGT GTCCGTGTCG TACACGGAAG ATGGCCAGCC CATCGTCCAG 
GTTCAGCAAC AGGTCCTGGA GGCCGCACAG CAGGCAGCCA GCGCTTCCGG AGGTGCAGAT 
GACAAGGAGG CGGCTGCCGA CTCGGCCGGC GAGGAAGTGC AAGTGTCGGA GGCATCTAGT 
GGCACGGCAT CCACCACCGT TGTTGCCACG AGCACGGCCA GCAGCACTGG TGGCCCCTCC 
GGCGGCAGCT TTTTTGCCCT GGAGGAGTTT ACGGAGACAA AGACCGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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pzg cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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