grim cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	grim
Entrez Gene ID	40014
Full Name	CG4345 gene product from transcript CG4345-RA
Synonyms	BcDNA:RE28551,CG4345,Dmel\CG4345,GRIM,Grim,gri,grm
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 75C4-75C4

mRNA and Protein(s)

mRNA	Protein	Name
NM_079413.3	NP_524137.2	grim

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Dm myb and grim are both involved in regulating PCD in two distinct settings suggests that these two genes may often work together to mediate PCD.
Title: The dREAM/Myb-MuvB complex and Grim are key regulators of the programmed death of neural precursor cells at the Drosophila posterior wing margin.

Study provide evidence that miR-6/11 functions through regulation of the proapoptotic genes, reaper (rpr), head involution defective (hid), grim and sickle (skl).
Title: Overlapping functions of microRNAs in control of apoptosis during Drosophila embryogenesis.

findings demonstrate that neuroblast apoptosis during embryogenesis requires the coordinated expression of the cell death genes grim and reaper, and possibly sickle
Title: Coordinated expression of cell death genes regulates neuroblast apoptosis.

Programmed cell death of microchaete glial cells represents an exceptional opportunity to study the mitochondrial proapoptotic process induced by Grim.
Title: grim promotes programmed cell death of Drosophila microchaete glial cells.

Rpr is able to self-associate and also binds to Hid and Grim.
Title: Drosophila IAP antagonists form multimeric complexes to promote cell death.

The following grim gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the grim cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa18855	NM_079413.3 Latest version!	Drosophila melanogaster grim (grim), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

grim ( NM_079413.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524137.2 Drosophila melanogaster grim (grim), mRNA.

Price & Availability↑

CloneID	OFa18855
Clone ID Related Accession (Same CDS sequence)	NM_079413.3
Accession Version	NM_079413.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	grim
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079413.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCATCG CCTATTTCAT ACCCGACCAG GCCCAATTGT TGGCCAGAAG CTATCAGCAA 
AACGGCCAGC AGACAGCAGC GAGTCCAAGG ACAACTGCAA CAGCTGCTGC ACCATCGCAG 
CAGCAGCAAC AATCGCAACA ACAGCAGCAG CAGCAGCGAC ATCATCATCA GCAACAGCGC 
CCACAATTCC GTGCCAATAT TTCCGTGCCG CTGGGAAGTC AACAGGGATC GATGACCATG 
TCGGAGTTTG GATGCTGGGA TCTTTTGGCC CAGATCTTCT GCTACGCTCT GCGAATCTAC 
AGCTACAGTT CGAGCCAGCG TCAACCGACG GTCATTCAGA TATCCTTCGA GATCAGCAGC 
GGCGGTCAGA ACAACGATGA GGACGACGTG ACTGATGCCA CCTCCAAGGA GAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524137.2
CDS	319..735
Translation	MAIAYFIPDQAQLLARSYQQNGQQTAASPRTTATAAAPSQQQQQSQQQQQQQRHHHQQQRPQFRANISVPLGSQQGSMTMSEFGCWDLLAQIFCYALRIYSYSSSQRQPTVIQISFEISSGGQNNDEDDVTDATSKEN

Target ORF information:

RefSeq Version	NM_079413.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster grim (grim), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079413.3

ORF Insert Sequence:

ATGGCCATCG CCTATTTCAT ACCCGACCAG GCCCAATTGT TGGCCAGAAG CTATCAGCAA 
AACGGCCAGC AGACAGCAGC GAGTCCAAGG ACAACTGCAA CAGCTGCTGC ACCATCGCAG 
CAGCAGCAAC AATCGCAACA ACAGCAGCAG CAGCAGCGAC ATCATCATCA GCAACAGCGC 
CCACAATTCC GTGCCAATAT TTCCGTGCCG CTGGGAAGTC AACAGGGATC GATGACCATG 
TCGGAGTTTG GATGCTGGGA TCTTTTGGCC CAGATCTTCT GCTACGCTCT GCGAATCTAC 
AGCTACAGTT CGAGCCAGCG TCAACCGACG GTCATTCAGA TATCCTTCGA GATCAGCAGC 
GGCGGTCAGA ACAACGATGA GGACGACGTG ACTGATGCCA CCTCCAAGGA GAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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grim cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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