Mip cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mip
Entrez Gene ID	39933
Full Name	Myoinhibiting peptide precursor
Synonyms	ASB,AST-B,Ast-B,AstB,AstB-1,CG6456,DAP-B,DmMIP1,DmMIP2,DmMIP3,DmMIP4,DmMIP5,Dmel\CG6456,Drm-MIP,Drm-MIP-1,Drm-MIP-2,Drm-MIP-3,Drm-MIP-4,Drm-MIP-5,MIP,MIP-1,MIP-2,MIP-3,MIP-4,MIP-5,mip
General protein information	Preferred Names Myoinhibiting peptide precursor Names CG6456 gene product from transcript CG6456-RA CG6456-PA Drostatin-B1 Drostatin-B3 Drostatin-B4 Drostatin-B5 Mip-PA Mip-like peptide allatostatin (B-type) allatostatin B allatostatin B-1 allatostatin cricket-type allatostatin-B allatostatin-B1 allostatin B drostatin-B2 mioinhibiting peptide precursor myoinhibiting peptide myoinhibiting peptide precursor myoinhibitory peptide
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 74A5-74A5

mRNA and Protein(s)

mRNA	Protein	Name
NM_140714.4	NP_648971.1	myoinhibiting peptide precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

MIP signaling in chemosensory neurons is an instructive signal adjusting female choice behavior to reproductive state.
Title: Neuropeptides Modulate Female Chemosensory Processing upon Mating in Drosophila.

MIP encodes anorexigenic brain signaling that determines body weight.MIP produces satiety responses independently of sex peptide receptor.MIP modulates behavioral responses to olfactory and gustatory cues of food.
Title: Identification of a Peptidergic Pathway Critical to Satiety Responses in Drosophila.

Our results illuminate how cell type specific versions of the Mip/dREAM complex and the general transcription machinery cooperate to drive selective gene activation during differentiation in stem cell lineages.
Title: Recruitment of Mediator Complex by Cell Type and Stage-Specific Factors Required for Tissue-Specific TAF Dependent Gene Activation in an Adult Stem Cell Lineage.

released centrally before and during night-time sleep, when the sleep drive is elevated
Title: A homeostatic sleep-stabilizing pathway in Drosophila composed of the sex peptide receptor and its ligand, the myoinhibitory peptide.

MIPs likely function as ancestral ligands of SPRs and could place evolutionary constraints on the MIP/SPR class.
Title: Myoinhibiting peptides are the ancestral ligands of the promiscuous Drosophila sex peptide receptor.

The following Mip gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mip cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa18810	NM_140714.4 Latest version!	Drosophila melanogaster myoinhibiting peptide precursor (Mip), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mip ( NM_140714.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648971.1 Drosophila melanogaster myoinhibiting peptide precursor (Mip), mRNA.

Price & Availability↑

CloneID	OFa18810
Clone ID Related Accession (Same CDS sequence)	NM_140714.4
Accession Version	NM_140714.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	myoinhibiting peptide precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140714.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTCACA CTAAGACGCG GAGAACTTAC GGCTTTCTGA TGGTGCTCCT CATCCTGGGC 
AGCGCTTGCG GTAATTTGGT GGCCAGTGGT AGTGCTGGCA GTCCACCCTC TAATGAACCA 
GGAGGCGGAG GCCTGTCCGA ACAGGTGGTA CTGGACCAAC TTAGCGAGAG CGACCTGTAC 
GGCAACAATA AGCGCGCCTG GCAGTCGCTG CAGAGCTCGT GGGGCAAGCG GTCCTCCAGC 
GGAGACGTCA GCGATCCGGA CATCTACATG ACCGGCCACT TTGTTCCGCT GGTAATCACG 
GATGGCACGA ACACCATCGA TTGGGACACC TTCGAGCGGC TGGCCAGTGG ACAATCCGCA 
CAGCAGCAGC AGCAACAGCC GCTCCAGCAG CAATCGCAAT CTGGCGAGGA TTTCGATGAT 
CTCGCAGGCG AGCCGGATGT GGAAAAGCGG GCCTGGAAGA GCATGAACGT CGCTTGGGGA 
AAGAGGAGGC AGGCGCAGGG CTGGAACAAG TTCAGGGGCG CCTGGGGTAA GCGCGAACCC 
ACATGGAACA ACCTGAAGGG CATGTGGGGC AAACGCGACC AGTGGCAGAA GCTCCACGGC 
GGCTGGGGCA AGCGCTCCCA GTTGCCCAGC AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648971.1
CDS	290..925
Translation	MAHTKTRRTYGFLMVLLILGSACGNLVASGSAGSPPSNEPGGGGLSEQVVLDQLSESDLYGNNKRAWQSLQSSWGKRSSSGDVSDPDIYMTGHFVPLVITDGTNTIDWDTFERLASGQSAQQQQQQPLQQQSQSGEDFDDLAGEPDVEKRAWKSMNVAWGKRRQAQGWNKFRGAWGKREPTWNNLKGMWGKRDQWQKLHGGWGKRSQLPSN

Target ORF information:

RefSeq Version	NM_140714.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster myoinhibiting peptide precursor (Mip), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140714.4

ORF Insert Sequence:

ATGGCTCACA CTAAGACGCG GAGAACTTAC GGCTTTCTGA TGGTGCTCCT CATCCTGGGC 
AGCGCTTGCG GTAATTTGGT GGCCAGTGGT AGTGCTGGCA GTCCACCCTC TAATGAACCA 
GGAGGCGGAG GCCTGTCCGA ACAGGTGGTA CTGGACCAAC TTAGCGAGAG CGACCTGTAC 
GGCAACAATA AGCGCGCCTG GCAGTCGCTG CAGAGCTCGT GGGGCAAGCG GTCCTCCAGC 
GGAGACGTCA GCGATCCGGA CATCTACATG ACCGGCCACT TTGTTCCGCT GGTAATCACG 
GATGGCACGA ACACCATCGA TTGGGACACC TTCGAGCGGC TGGCCAGTGG ACAATCCGCA 
CAGCAGCAGC AGCAACAGCC GCTCCAGCAG CAATCGCAAT CTGGCGAGGA TTTCGATGAT 
CTCGCAGGCG AGCCGGATGT GGAAAAGCGG GCCTGGAAGA GCATGAACGT CGCTTGGGGA 
AAGAGGAGGC AGGCGCAGGG CTGGAACAAG TTCAGGGGCG CCTGGGGTAA GCGCGAACCC 
ACATGGAACA ACCTGAAGGG CATGTGGGGC AAACGCGACC AGTGGCAGAA GCTCCACGGC 
GGCTGGGGCA AGCGCTCCCA GTTGCCCAGC AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Mip cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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