tra cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	tra
Entrez Gene ID	39849
Full Name	transformer
Synonyms	CG16724,Dmel\CG16724,Dmtra,TRA,Tra
General protein information	Preferred Names transformer Names CG16724 gene product from transcript CG16724-RA CG16724-PA CG16724-PB tra-PA tra-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 73A10-73A10\|3-45 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_079390.3	NP_524114.1	transformer, isoform A
NM_001014588.2	NP_001014588.1	transformer, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

tra expression play crucial role in determining sex-specific size in Drosophila larvae and adult tissue.
Title: Sexual Dimorphism of Body Size Is Controlled by Dosage of the

body size is regulated by tra in a sex-specific fashion.
Title: The Sex Determination Gene transformer Regulates Male-Female Differences in Drosophila Body Size.

When tsh(GAL4) drove expression of transformer (tra), males were unable to produce prolonged wing extensions.
Title: Genetic feminization of the thoracic nervous system disrupts courtship song in male Drosophila melanogaster.

Effect of tra of Tephritidae on the somatic sexual development of Drosophila are reported.
Title: Effect of the gene transformer of Anastrepha on the somatic sexual development of Drosophila.

the reciprocal regulatory effect by studying genetically sensitized females: TRA-F from either maternal or zygotic tra expression stimulates sex-lethal -positive autoregulation.
Title: Sexual back talk with evolutionary implications: stimulation of the Drosophila sex-determination gene sex-lethal by its target transformer.

The following tra gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tra cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa18719	NM_001014588.2 Latest version!	Drosophila melanogaster transformer (tra), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa18718	NM_079390.3 Latest version!	Drosophila melanogaster transformer (tra), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tra ( NM_001014588.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001014588.1 Drosophila melanogaster transformer (tra), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa18719
Clone ID Related Accession (Same CDS sequence)	NM_001014588.2
Accession Version	NM_001014588.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 111bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	transformer, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On May 8, 2012 this sequence version replaced NM_001014588.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

1 61	ATGAAAATGG ATGCCGACAG CAGTGGAACC CAGCATCGAG TGTCATATTG TGTGAAATGT GAAATGGATG AGAATGAACG CTGGACGACC AGACAGAGAA AGAGAAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014588.1
CDS	8..118
Translation	MKMDADSSGTQHRVSYCVKCEMDENERWTTRQRKRS

Target ORF information:

RefSeq Version	NM_001014588.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster transformer (tra), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014588.2

ORF Insert Sequence:

1 61	ATGAAAATGG ATGCCGACAG CAGTGGAACC CAGCATCGAG TGTCATATTG TGTGAAATGT GAAATGGATG AGAATGAACG CTGGACGACC AGACAGAGAA AGAGAAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tra ( NM_079390.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524114.1 Drosophila melanogaster transformer (tra), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa18718
Clone ID Related Accession (Same CDS sequence)	NM_079390.3
Accession Version	NM_079390.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	transformer, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On May 8, 2012 this sequence version replaced NM_079390.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAATGG ATGCCGACAG CAGTGGAACC CAGCATCGAG ATTCCCGTGG CTCAAGGTCT 
CGATCCCGGC GAGAAAGAGA ATACCATGGG CGATCAAGCG AGAGGGACAG CAGAAAGAAG 
GAGCACAAGA TTCCGTACTT TGCAGACGAG GTTCGAGAAC AGGATCGGTT GAGAAGATTG 
CGCCAAAGAG CGCACCAATC CACCAGACGC ACTCGCTCCA GATCCAGATC ACAGTCGTCC 
ATCAGAGAGA GCAGGCACAG AAGGCATCGC CAGCGCTCTA GGAGCCGCAA TCGCAACCGA 
AGTCGCAGCA GTGAACGAAA ACGCCGTCAA CATAGCCGAA GTCGCAGCAG TGAACGAAGA 
CGCCGTCAAC GGAGCCCGCA TCGGTATAAT CCTCCGCCAA AGATCATCAA CTACTATGTG 
CAAGTGCCAC CACAGGATTT CTACGGGATG TCTGGTATGC AGCAAAGTTT TGGATACCAA 
AGGCTACCAC GTCCTCCGCC GTTTCCACCG GCCCCCTACA GATACCGCCA GCGACCGCCG 
TTCATTGGAG TTCCGCGATT TGGCTACAGA AACGCGGGGC GTCCCCCATA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524114.1
CDS	8..601
Translation	MKMDADSSGTQHRDSRGSRSRSRREREYHGRSSERDSRKKEHKIPYFADEVREQDRLRRLRQRAHQSTRRTRSRSRSQSSIRESRHRRHRQRSRSRNRNRSRSSERKRRQHSRSRSSERRRRQRSPHRYNPPPKIINYYVQVPPQDFYGMSGMQQSFGYQRLPRPPPFPPAPYRYRQRPPFIGVPRFGYRNAGRPPY

Target ORF information:

RefSeq Version	NM_079390.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster transformer (tra), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079390.3

ORF Insert Sequence:

ATGAAAATGG ATGCCGACAG CAGTGGAACC CAGCATCGAG ATTCCCGTGG CTCAAGGTCT 
CGATCCCGGC GAGAAAGAGA ATACCATGGG CGATCAAGCG AGAGGGACAG CAGAAAGAAG 
GAGCACAAGA TTCCGTACTT TGCAGACGAG GTTCGAGAAC AGGATCGGTT GAGAAGATTG 
CGCCAAAGAG CGCACCAATC CACCAGACGC ACTCGCTCCA GATCCAGATC ACAGTCGTCC 
ATCAGAGAGA GCAGGCACAG AAGGCATCGC CAGCGCTCTA GGAGCCGCAA TCGCAACCGA 
AGTCGCAGCA GTGAACGAAA ACGCCGTCAA CATAGCCGAA GTCGCAGCAG TGAACGAAGA 
CGCCGTCAAC GGAGCCCGCA TCGGTATAAT CCTCCGCCAA AGATCATCAA CTACTATGTG 
CAAGTGCCAC CACAGGATTT CTACGGGATG TCTGGTATGC AGCAAAGTTT TGGATACCAA 
AGGCTACCAC GTCCTCCGCC GTTTCCACCG GCCCCCTACA GATACCGCCA GCGACCGCCG 
TTCATTGGAG TTCCGCGATT TGGCTACAGA AACGCGGGGC GTCCCCCATA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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tra cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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