brm cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following brm gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the brm cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa18620 NM_001274978.1
Latest version!
Drosophila melanogaster brahma (brm), transcript variant F, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OFa18621 NM_001274977.1
Latest version!
Drosophila melanogaster brahma (brm), transcript variant E, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OFa20961 NM_168640.2
Latest version!
Drosophila melanogaster brahma (brm), transcript variant D, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OFa20961 NM_080497.5
Latest version!
Drosophila melanogaster brahma (brm), transcript variant C, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OFa20962 NM_080498.3
Latest version!
Drosophila melanogaster brahma (brm), transcript variant A, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OFa20962 NM_168641.1
Latest version!
Drosophila melanogaster brahma (brm), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa18620
    Clone ID Related Accession (Same CDS sequence) NM_001274978.1
    Accession Version NM_001274978.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4929bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product brahma, isoform F
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCCTCGC CCTCTCCGGC GAACAGTCCC ATGCCACCGC CACAGGCGCC CAGTCCGATG 
    GCCCCGCCCT CGCAGAGTCC CGCCCCCTCG CCGCACAGCC CGTATCCGCA CCAGCAGCCG
    GGTCCACTGC AAGGACCTCC GCCACCCGGA CATCCCGGTG CCTACGGACA TCCCATGCAG
    CACGGACCAC CCGGCCAGGG TCCACCCGGG CATCATATGC CACCGCACCA CCAGGGGATG
    AGTCCGCACA TGGGAATGCA GATGCCGCCG ACGGGACCGA ACATGTCCCC CTACCAAACC
    CACGGAATGC CACCCAATCT TTTGTGCAAA ATGGAATTTG TAGCACCCAC ACAACCCTGC
    ATAGTGTCAC CTGGTGGCCC ACCTGGAGGC CCGCCACCTC CGGAGCGCTC GAGCCAGGAG
    AACCTGCACG CGCTTCAACG CGCCATTGAT TCAATGGAGG AAAAGGGCTT GCAAGAGGAT
    CCTCGCTACT CGCAGCTGCT GGCAATGCGA GCAACCTCGA AGCATCAGCA CCTTAATGGC
    AATCAGGTTA ACTTGTTGCG CACACAGATC ACCGCCTATC GGTTGCTGGC GCGGAACAAG
    CCCATATCCA TGCAGATGCA GCAGGCGTTG CAGGCCGCAC AGCAACAGCC ACCGCCAGGA
    CCTCCAATTG GGCCACCTGG AGCACCAGGC GGACCGCCAC CAGGGAGCCA GCATGCAGGA
    CAACCGCCCG TGCCGCCGCA ACAACAGCAA CAACCGCCAC CTTCAGCAGG AACTCCACCC
    CAGTGCTCCA CGCCGCCCGC AAGCAATCCT TATGGACCGC CAGTTCCTGG CCAGAAAATG
    CAGGTAGCGC CACCACCGCC ACACATGCAA CAGGGACAGC CATTGCCACC ACAACCGCCA
    CAAGTGGGTG GACCCCCGCC TATACAACAG CAGCAACCAC CTCAGCAGCA GCAACAACAG
    TCGCAGCCCC CGCCACCGGA GCCACATCAG CACCAGTTGC CAAACGGAGG CAAACCCCTG
    AGTATGGGGC CATCTGGCGG GCAACCACTA ATACCTTCAT CACCCATGCA GCCACAAGTG
    AGAGGGACAC TGCCAGGAAT GCCGCCAGGT AGTCAGGTTC CGCAACCAGG AGGAGGTCCT
    CAGCGACAGG TACCACCTGC GGGCATGCCG ATGCCCAAGC CCAATCGCAT TACAACGGTG
    GCCAAACCCG TCGGTCTGGA TCCCATAACA TTGCTGCAGG AAAGGGAGAA TAGGATAGCA
    GCCAGGATAT CATTGCGCAT GCAGGAGTTG CAGCGACTGC CGGCCACGAT GTCCGAGGAT
    CTGCGTCTGC AAGCGGCTAT CGAGTTGAGG GCCCTAAGGG TCTTGAACTT CCAGCGACAG
    CTGCGTATGG AGTTTGTTCA GTGCACGAGA CGAGATACCA CTTTGGAAAC AGCGTTAAAC
    ATTAAGCTCT ACAAGAGGAC CAAGAGGCAG GGCCTGCGGG AGGCACGTGC CACCGAGAAG
    CTAGAGAAGC AACAGAAGCT AGAGGCTGAA CGAAAGCGTC GCCAAAAGCA TCTGGAGTTC
    TTGGCTGCTG TGCTTCAGCA CGGCAAGGAT CTAAGGGAGT TCCACAGAAA TAATAAGGCC
    CAGCTTGCAA GGATGAACAA GGCCGTGATG AATCACCACG CCAATGCCGA GCGTGAACAG
    AAGAAGGAAC AAGAGCGCAT CGAAAAGGAA CGTATGCGAC GTCTGATGGC CGAGGATGAG
    GAGGGTTACC GCAAGCTGAT TGATCAAAAG AAGGACAAGA GATTGGCTTT CCTACTGTCG
    CAGACAGATG AGTATATTAG CAACCTAACT CAGATGGTGA AACAGCACAA GGACGACCAG
    ATGAAGAAAA AGGAGGAGGA GGGCAAGCGG CTGATACAGT TCAAAAAGGA GCTGCTGATG
    AGCGGAGAGT ACATTGGCAT TGATGAGGGC AGCATTGTTG CCGATATGCG GGTACATGTG
    GTGGAACAGT GCACGGGTAA GAAACTCACC GGCGATGATG CACCCATGCT GAAGCACTTG
    CACCGCTGGC TGAATATGCA TCCTGGCTGG GATTGGATCG ACGACGAGGA GGACAGCTGT
    GGGAGCAACG ACGATCATAA ACCCAAGGTG GAGGAGCAAC CAACGGCAAC AGAAGATGCC
    ACCGACAAAG CGCAGGCGAC GGGCAACGAT GAGGATGCCA AGGATCTAAT TACCAAGGCC
    AAGGTGGAGG ACGATGAGTA CAGGACGGAG GAACAGACGT ACTACAGCAT CGCTCATACC
    ATTCATGAAA AGGTTGTGGA GCAGGCCAGC ATCATGGTTA ATGGTACGCT CAAGGAATAC
    CAGATCAAAG GCTTGGAGTG GTTGGTTTCG CTGTACAATA ACAATCTAAA TGGTATTTTG
    GCCGATGAAA TGGGTTTGGG TAAAACCATT CAAACCATTT CGCTGGTAAC CTACCTTATG
    GATCGAAAGA AGGTTATGGG TCCTTACTTG ATTATTGTAC CACTCTCCAC CCTGCCCAAT
    TGGGTGCTGG AATTCGAGAA GTGGGCGCCA GCCGTGGGTG TAGTCAGCTA CAAGGGCAGT
    CCGCAGGGAA GGCGATTGCT GCAAAATCAA ATGAGAGCAA CCAAGTTCAA TGTGCTGTTA
    ACCACATATG AGTACGTGAT TAAGGACAAG GCGGTGCTGG CCAAGATCCA GTGGAAATAC
    ATGATTATCG ACGAGGGTCA CCGCATGAAG AACCATCACT GCAAACTCAC CCAGGTGCTG
    AACACCCACT ACATTGCCCC CTATCGGTTA CTTCTCACGG GTACACCTCT GCAAAACAAG
    CTTCCCGAGT TGTGGGCGCT ACTTAATTTC CTGCTGCCCT CGATCTTTAA GTCCTGCTCC
    ACATTTGAAC AGTGGTTCAA TGCGCCATTT GCCACCACTG GCGAGAAGGT CGAGCTAAAC
    GAGGAGGAGA CCATCCTTAT CATTCGTCGT CTGCACAAGG TTCTTCGTCC TTTCCTGTTG
    CGTCGCCTCA AAAAGGAGGT AGAGCACCAG CTGCCCGACA AGGTCGAGTA CATCATCAAG
    TGCGACATGT CCGCGCTGCA ACGCGTTCTC TACAAGCACA TGCAGAGCAA GGGTGTCCTG
    CTCACCGATG GTTCCGAGAA GGGCAAGCAC GGCAAGGGAG GCGCTAAGGC GCTGATGAAC
    ACGATCGTAC AGCTGCGCAA GCTGTGCAAC CATCCGTTTA TGTTCCAGCA CATAGAAGAG
    AAGTACTGCG ATCACACTGG CGGCCACGGA GTTGTATCCG GACCGGATCT GTATCGTGTC
    TCTGGCAAAT TCGAGCTGCT TGATCGCATC CTGCCCAAGC TGAAGGCGAC CAATCATCGC
    GTGCTGCTCT TCTGTCAGAT GACCCAATGC ATGACCATCA TCGAAGACTA CCTGGGATGG
    CGCCAATTCG GCTACCTTCG ACTCGACGGT ACCACGAAGG CCGAGGATCG TGGCGAACTA
    CTGCGAAAAT TCAACGCCAA AGGCTCGGAT GTATTTGTCT TTTTGCTATC TACCCGAGCT
    GGTGGTTTGG GTCTTAATCT GCAGACCGCC GATACTGTAG TAATCTTCGA CTCCGATTGG
    AATCCCCATC AGGATCTGCA AGCCCAGGAT CGTGCCCATC GTATTGGTCA ACGTAACGAG
    GTGCGCGTCC TGCGACTAAT GACAGTCAAC TCGGTGGAAG AGCGCATCTT GGCAGCAGCC
    AGGTACAAGC TGAACATGGA CGAGAAGGTC ATCCAGGCTG GTATGTTCGA TCAGAAGTCA
    ACGGGTAGCG AGCGACAGCA GTTCCTACAA ACGATTCTGC ACCAGGATGA CAACGAAGAG
    GAGGAGGAAA ATGAGGTGCC CGATGACGAA ATGATCAACA TGATGATCGC CCGCAGCGAG
    GAGGAGATTG AAATATTTAA GCGCATGGAT GCTGAGCGCA AGAAGGAGGA TGAGGAGATC
    CATCCGGGTA GGGAACGTCT GATCGATGAG TCCGAGCTAC CCGACTGGTT GACAAAGGAT
    GATGACGAAG TAGAGCGTTT CCACTATCAG TACGACGAGG ATACCATTCT AGGTCGAGGC
    TCCCGGCAGC GAAAGGAAGT AGACTACACC GATAGTTTGA CTGAAAAGGA GTGGCTGAAA
    GCCATCGACG ATGGCGCTGA GTTCGACGAG GAAGAGGAGG AAGACGATTC GAAGCGCAAG
    CGACGAAAGC GTAAGAATCG GAAGGAAGAG TCCGACGATG ATTCGTTGAT CCTAAAACGG
    CGACGACGCC AAAATCTGGA CAAGCGGTCC AAGAAGCAGA TGCACAAGAT TATGAGTGCG
    GTTATCAAGC ACAACCAAGA TGGACGAACC CTGTCCGAAC CGTTCATGAA GTTGCCTTCG
    CGGCAGAGGT TGCCCGACTA CTACGAGATC ATTAAGCGGC CAGTTGATAT TAAGAAAATC
    CTGCAGCGCA TTGAGGACTG CAAGTATGCC GATCTCAACG AGCTGGAGAA GGACTTTATG
    CAGCTATGCC AGAATGCACA GATCTACAAC GAAGAGGCGT CTCTGATTTA CCTGGACTCG
    ATTGCCCTGC AAAAGGTATT CGTTGGGGCC AGGCAGAGGA TAACAGCTGC AGCGGATGCA
    GCCGCAGTGG CAGCCGGAGA TAACACGGGT GAAGCGCACG GCAATGGAGG TTCGGATAAT
    TCGGACAACG ACGACGACGA TGGTGGCGAT GACGGCTCCG ACGACGAGGA GATTGCAACC
    ACTTCAGCGG CGGCTGTCAA AATGAAGCTG AAACTGAACA AATCTCTGGC TAGTGCGCCC
    GCTACGCCCA CGCAATCATC GTCGAATGTG AGCAGCGGAG CGGCTACCAC ATCCAAGAAG
    CAGACGCGTC GCAAGCGCTC CCAAAAGAAG TACACTATTT CAGACGATGA CGACGATGAC
    ATGGACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001261907.1
    CDS128..5056
    Translation

    Target ORF information:

    RefSeq Version NM_001274978.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster brahma (brm), transcript variant F, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001274978.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCCTCGC CCTCTCCGGC GAACAGTCCC ATGCCACCGC CACAGGCGCC CAGTCCGATG 
    GCCCCGCCCT CGCAGAGTCC CGCCCCCTCG CCGCACAGCC CGTATCCGCA CCAGCAGCCG
    GGTCCACTGC AAGGACCTCC GCCACCCGGA CATCCCGGTG CCTACGGACA TCCCATGCAG
    CACGGACCAC CCGGCCAGGG TCCACCCGGG CATCATATGC CACCGCACCA CCAGGGGATG
    AGTCCGCACA TGGGAATGCA GATGCCGCCG ACGGGACCGA ACATGTCCCC CTACCAAACC
    CACGGAATGC CACCCAATCT TTTGTGCAAA ATGGAATTTG TAGCACCCAC ACAACCCTGC
    ATAGTGTCAC CTGGTGGCCC ACCTGGAGGC CCGCCACCTC CGGAGCGCTC GAGCCAGGAG
    AACCTGCACG CGCTTCAACG CGCCATTGAT TCAATGGAGG AAAAGGGCTT GCAAGAGGAT
    CCTCGCTACT CGCAGCTGCT GGCAATGCGA GCAACCTCGA AGCATCAGCA CCTTAATGGC
    AATCAGGTTA ACTTGTTGCG CACACAGATC ACCGCCTATC GGTTGCTGGC GCGGAACAAG
    CCCATATCCA TGCAGATGCA GCAGGCGTTG CAGGCCGCAC AGCAACAGCC ACCGCCAGGA
    CCTCCAATTG GGCCACCTGG AGCACCAGGC GGACCGCCAC CAGGGAGCCA GCATGCAGGA
    CAACCGCCCG TGCCGCCGCA ACAACAGCAA CAACCGCCAC CTTCAGCAGG AACTCCACCC
    CAGTGCTCCA CGCCGCCCGC AAGCAATCCT TATGGACCGC CAGTTCCTGG CCAGAAAATG
    CAGGTAGCGC CACCACCGCC ACACATGCAA CAGGGACAGC CATTGCCACC ACAACCGCCA
    CAAGTGGGTG GACCCCCGCC TATACAACAG CAGCAACCAC CTCAGCAGCA GCAACAACAG
    TCGCAGCCCC CGCCACCGGA GCCACATCAG CACCAGTTGC CAAACGGAGG CAAACCCCTG
    AGTATGGGGC CATCTGGCGG GCAACCACTA ATACCTTCAT CACCCATGCA GCCACAAGTG
    AGAGGGACAC TGCCAGGAAT GCCGCCAGGT AGTCAGGTTC CGCAACCAGG AGGAGGTCCT
    CAGCGACAGG TACCACCTGC GGGCATGCCG ATGCCCAAGC CCAATCGCAT TACAACGGTG
    GCCAAACCCG TCGGTCTGGA TCCCATAACA TTGCTGCAGG AAAGGGAGAA TAGGATAGCA
    GCCAGGATAT CATTGCGCAT GCAGGAGTTG CAGCGACTGC CGGCCACGAT GTCCGAGGAT
    CTGCGTCTGC AAGCGGCTAT CGAGTTGAGG GCCCTAAGGG TCTTGAACTT CCAGCGACAG
    CTGCGTATGG AGTTTGTTCA GTGCACGAGA CGAGATACCA CTTTGGAAAC AGCGTTAAAC
    ATTAAGCTCT ACAAGAGGAC CAAGAGGCAG GGCCTGCGGG AGGCACGTGC CACCGAGAAG
    CTAGAGAAGC AACAGAAGCT AGAGGCTGAA CGAAAGCGTC GCCAAAAGCA TCTGGAGTTC
    TTGGCTGCTG TGCTTCAGCA CGGCAAGGAT CTAAGGGAGT TCCACAGAAA TAATAAGGCC
    CAGCTTGCAA GGATGAACAA GGCCGTGATG AATCACCACG CCAATGCCGA GCGTGAACAG
    AAGAAGGAAC AAGAGCGCAT CGAAAAGGAA CGTATGCGAC GTCTGATGGC CGAGGATGAG
    GAGGGTTACC GCAAGCTGAT TGATCAAAAG AAGGACAAGA GATTGGCTTT CCTACTGTCG
    CAGACAGATG AGTATATTAG CAACCTAACT CAGATGGTGA AACAGCACAA GGACGACCAG
    ATGAAGAAAA AGGAGGAGGA GGGCAAGCGG CTGATACAGT TCAAAAAGGA GCTGCTGATG
    AGCGGAGAGT ACATTGGCAT TGATGAGGGC AGCATTGTTG CCGATATGCG GGTACATGTG
    GTGGAACAGT GCACGGGTAA GAAACTCACC GGCGATGATG CACCCATGCT GAAGCACTTG
    CACCGCTGGC TGAATATGCA TCCTGGCTGG GATTGGATCG ACGACGAGGA GGACAGCTGT
    GGGAGCAACG ACGATCATAA ACCCAAGGTG GAGGAGCAAC CAACGGCAAC AGAAGATGCC
    ACCGACAAAG CGCAGGCGAC GGGCAACGAT GAGGATGCCA AGGATCTAAT TACCAAGGCC
    AAGGTGGAGG ACGATGAGTA CAGGACGGAG GAACAGACGT ACTACAGCAT CGCTCATACC
    ATTCATGAAA AGGTTGTGGA GCAGGCCAGC ATCATGGTTA ATGGTACGCT CAAGGAATAC
    CAGATCAAAG GCTTGGAGTG GTTGGTTTCG CTGTACAATA ACAATCTAAA TGGTATTTTG
    GCCGATGAAA TGGGTTTGGG TAAAACCATT CAAACCATTT CGCTGGTAAC CTACCTTATG
    GATCGAAAGA AGGTTATGGG TCCTTACTTG ATTATTGTAC CACTCTCCAC CCTGCCCAAT
    TGGGTGCTGG AATTCGAGAA GTGGGCGCCA GCCGTGGGTG TAGTCAGCTA CAAGGGCAGT
    CCGCAGGGAA GGCGATTGCT GCAAAATCAA ATGAGAGCAA CCAAGTTCAA TGTGCTGTTA
    ACCACATATG AGTACGTGAT TAAGGACAAG GCGGTGCTGG CCAAGATCCA GTGGAAATAC
    ATGATTATCG ACGAGGGTCA CCGCATGAAG AACCATCACT GCAAACTCAC CCAGGTGCTG
    AACACCCACT ACATTGCCCC CTATCGGTTA CTTCTCACGG GTACACCTCT GCAAAACAAG
    CTTCCCGAGT TGTGGGCGCT ACTTAATTTC CTGCTGCCCT CGATCTTTAA GTCCTGCTCC
    ACATTTGAAC AGTGGTTCAA TGCGCCATTT GCCACCACTG GCGAGAAGGT CGAGCTAAAC
    GAGGAGGAGA CCATCCTTAT CATTCGTCGT CTGCACAAGG TTCTTCGTCC TTTCCTGTTG
    CGTCGCCTCA AAAAGGAGGT AGAGCACCAG CTGCCCGACA AGGTCGAGTA CATCATCAAG
    TGCGACATGT CCGCGCTGCA ACGCGTTCTC TACAAGCACA TGCAGAGCAA GGGTGTCCTG
    CTCACCGATG GTTCCGAGAA GGGCAAGCAC GGCAAGGGAG GCGCTAAGGC GCTGATGAAC
    ACGATCGTAC AGCTGCGCAA GCTGTGCAAC CATCCGTTTA TGTTCCAGCA CATAGAAGAG
    AAGTACTGCG ATCACACTGG CGGCCACGGA GTTGTATCCG GACCGGATCT GTATCGTGTC
    TCTGGCAAAT TCGAGCTGCT TGATCGCATC CTGCCCAAGC TGAAGGCGAC CAATCATCGC
    GTGCTGCTCT TCTGTCAGAT GACCCAATGC ATGACCATCA TCGAAGACTA CCTGGGATGG
    CGCCAATTCG GCTACCTTCG ACTCGACGGT ACCACGAAGG CCGAGGATCG TGGCGAACTA
    CTGCGAAAAT TCAACGCCAA AGGCTCGGAT GTATTTGTCT TTTTGCTATC TACCCGAGCT
    GGTGGTTTGG GTCTTAATCT GCAGACCGCC GATACTGTAG TAATCTTCGA CTCCGATTGG
    AATCCCCATC AGGATCTGCA AGCCCAGGAT CGTGCCCATC GTATTGGTCA ACGTAACGAG
    GTGCGCGTCC TGCGACTAAT GACAGTCAAC TCGGTGGAAG AGCGCATCTT GGCAGCAGCC
    AGGTACAAGC TGAACATGGA CGAGAAGGTC ATCCAGGCTG GTATGTTCGA TCAGAAGTCA
    ACGGGTAGCG AGCGACAGCA GTTCCTACAA ACGATTCTGC ACCAGGATGA CAACGAAGAG
    GAGGAGGAAA ATGAGGTGCC CGATGACGAA ATGATCAACA TGATGATCGC CCGCAGCGAG
    GAGGAGATTG AAATATTTAA GCGCATGGAT GCTGAGCGCA AGAAGGAGGA TGAGGAGATC
    CATCCGGGTA GGGAACGTCT GATCGATGAG TCCGAGCTAC CCGACTGGTT GACAAAGGAT
    GATGACGAAG TAGAGCGTTT CCACTATCAG TACGACGAGG ATACCATTCT AGGTCGAGGC
    TCCCGGCAGC GAAAGGAAGT AGACTACACC GATAGTTTGA CTGAAAAGGA GTGGCTGAAA
    GCCATCGACG ATGGCGCTGA GTTCGACGAG GAAGAGGAGG AAGACGATTC GAAGCGCAAG
    CGACGAAAGC GTAAGAATCG GAAGGAAGAG TCCGACGATG ATTCGTTGAT CCTAAAACGG
    CGACGACGCC AAAATCTGGA CAAGCGGTCC AAGAAGCAGA TGCACAAGAT TATGAGTGCG
    GTTATCAAGC ACAACCAAGA TGGACGAACC CTGTCCGAAC CGTTCATGAA GTTGCCTTCG
    CGGCAGAGGT TGCCCGACTA CTACGAGATC ATTAAGCGGC CAGTTGATAT TAAGAAAATC
    CTGCAGCGCA TTGAGGACTG CAAGTATGCC GATCTCAACG AGCTGGAGAA GGACTTTATG
    CAGCTATGCC AGAATGCACA GATCTACAAC GAAGAGGCGT CTCTGATTTA CCTGGACTCG
    ATTGCCCTGC AAAAGGTATT CGTTGGGGCC AGGCAGAGGA TAACAGCTGC AGCGGATGCA
    GCCGCAGTGG CAGCCGGAGA TAACACGGGT GAAGCGCACG GCAATGGAGG TTCGGATAAT
    TCGGACAACG ACGACGACGA TGGTGGCGAT GACGGCTCCG ACGACGAGGA GATTGCAACC
    ACTTCAGCGG CGGCTGTCAA AATGAAGCTG AAACTGAACA AATCTCTGGC TAGTGCGCCC
    GCTACGCCCA CGCAATCATC GTCGAATGTG AGCAGCGGAG CGGCTACCAC ATCCAAGAAG
    CAGACGCGTC GCAAGCGCTC CCAAAAGAAG TACACTATTT CAGACGATGA CGACGATGAC
    ATGGACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa18621
    Clone ID Related Accession (Same CDS sequence) NM_001274977.1
    Accession Version NM_001274977.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4977bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product brahma, isoform E
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCCTCGC CCTCTCCGGC GAACAGTCCC ATGCCACCGC CACAGGCGCC CAGTCCGATG 
    GCCCCGCCCT CGCAGAGTCC CGCCCCCTCG CCGCACAGCC CGTATCCGCA CCAGCAGCCG
    GGTCCACTGC AAGGACCTCC GCCACCCGGA CATCCCGGTG CCTACGGACA TCCCATGCAG
    CACGGACCAC CCGGCCAGGG TCCACCCGGG CATCATATGC CACCGCACCA CCAGGGGATG
    ATTTTCTCAA AAGGTCCGCA CATGGGAATG CAGATGCCGC CGACGGGACC GAACATGTCC
    CCCTACCAAA CCCACGGAAT GCCACCCAAT GCACCCACAC AACCCTGCAT AGTGTCACCT
    GGTGGCCCAC CTGGAGGCCC GCCACCTCCG GAGCGCTCGA GCCAGGAGAA CCTGCACGCG
    CTTCAACGCG CCATTGATTC AATGGAGGAA AAGGGCTTGC AAGAGGATCC TCGCTACTCG
    CAGCTGCTGG CAATGCGAGC AACCTCGAAG CATCAGCACC TTAATGGCAA TCAGGTTAAC
    TTGTTGCGCA CACAGATCAC CGCCTATCGG TTGCTGGCGC GGAACAAGCC CATATCCATG
    CAGATGCAGC AGGCGTTGCA GGCCGCACAG CAACAGCCAC CGCCAGGACC TCCAATTGGG
    CCACCTGGAG CACCAGGCGG ACCGCCACCA GGGAGCCAGC ATGCAGGACA ACCGCCCGTG
    CCGCCGCAAC AACAGCAACA ACCGCCACCT TCAGCAGGAA CTCCACCCCA GTGCTCCACG
    CCGCCCGCAA GCAATCCTTA TGGACCGCCA GTTCCTGGCC AGAAAATGCA GGTAGCGCCA
    CCACCGCCAC ACATGCAACA GGGACAGCCA TTGCCACCAC AACCGCCACA AGTGGGTGGA
    CCCCCGCCTA TACAACAGCA GCAACCACCT CAGCAGCAGC AACAACAGTC GCAGCCCCCG
    CCACCGGAGC CACATCAGCA CCAGTTGCCA AACGGAGGCA AACCCCTGAG TATGGGGCCA
    TCTGGCGGGC AACCACTAAT ACCTTCATCA CCCATGCAGC CACAAGTGAG AGGGACACTG
    CCAGGAATGC CGCCAGGTAG TCAGGTTCCG CAACCAGGAG GAGGTCCTCA GCGACAGGTA
    CCACCTGCGG GCATGCCGAT GCCCAAGCCC AATCGCATTA CAACGGTGGC CAAACCCGTC
    GGTCTGGATC CCATAACATT GCTGCAGGAA AGGGAGAATA GGATAGCAGC CAGGATATCA
    TTGCGCATGC AGGAGTTGCA GCGACTGCCG GCCACGATGT CCGAGGATCT GCGTCTGCAA
    GCGGCTATCG AGTTGAGGGC CCTAAGGGTC TTGAACTTCC AGCGACAGCT GCGTATGGAG
    TTTGTTCAGT GCACGAGACG AGATACCACT TTGGAAACAG CGTTAAACAT TAAGCTCTAC
    AAGAGGACCA AGAGGCAGGG CCTGCGGGAG GCACGTGCCA CCGAGAAGCT AGAGAAGCAA
    CAGAAGCTAG AGGCTGAACG AAAGCGTCGC CAAAAGCATC TGGAGTTCTT GGCTGCTGTG
    CTTCAGCACG GCAAGGATCT AAGGGAGTTC CACAGAAATA ATAAGGCCCA GCTTGCAAGG
    ATGAACAAGG CCGTGATGAA TCACCACGCC AATGCCGAGC GTGAACAGAA GAAGGAACAA
    GAGCGCATCG AAAAGGAACG TATGCGACGT CTGATGGCCG AGGATGAGGA GGGTTACCGC
    AAGCTGATTG ATCAAAAGAA GGACAAGAGA TTGGCTTTCC TACTGTCGCA GACAGATGAG
    TATATTAGCA ACCTAACTCA GATGGTGAAA CAGCACAAGG ACGACCAGAT GAAGAAAAAG
    GAGGAGGAGG GCAAGCGGCT GATACAGTTC AAAAAGGAGC TGCTGATGAG CGGAGAGTAC
    ATTGGCATTG ATGAGGGCAG CATTGTTGCC GATATGCGGG TACATGTGGT GGAACAGTGC
    ACGGGTAAGA AACTCACCGG CGATGATGCA CCCATGCTGA AGCACTTGCA CCGCTGGCTG
    AATATGCATC CTGGCTGGGA TTGGATCGAC GACGAGGAGG ACAGCTGTGG GAGCAACGAC
    GATCATAAAC CCAAGGTGGA GGAGCAACCA ACGGCAACAG AAGATGCCAC CGACAAAGCG
    CAGGCGACGG GCAACGATGA GGATGCCAAG GATCTAATTA CCAAGGCCAA GGTGGAGGAC
    GATGAGTACA GGACGGAGGA ACAGACGTAC TACAGCATCG CTCATACCAT TCATGAAAAG
    GTTGTGGAGC AGGCCAGCAT CATGGTTAAT GGTACGCTCA AGGAATACCA GATCAAAGGC
    TTGGAGTGGT TGGTTTCGCT GTACAATAAC AATCTAAATG GTATTTTGGC CGATGAAATG
    GGTTTGGGTA AAACCATTCA AACCATTTCG CTGGTAACCT ACCTTATGGA TCGAAAGAAG
    GTTATGGGTC CTTACTTGAT TATTGTACCA CTCTCCACCC TGCCCAATTG GGTGCTGGAA
    TTCGAGAAGT GGGCGCCAGC CGTGGGTGTA GTCAGCTACA AGGGCAGTCC GCAGGGAAGG
    CGATTGCTGC AAAATCAAAT GAGAGCAACC AAGTTCAATG TGCTGTTAAC CACATATGAG
    TACGTGATTA AGGACAAGGC GGTGCTGGCC AAGATCCAGT GGAAATACAT GATTATCGAC
    GAGGGTCACC GCATGAAGAA CCATCACTGC AAACTCACCC AGGTGCTGAA CACCCACTAC
    ATTGCCCCCT ATCGGTTACT TCTCACGGGT ACACCTCTGC AAAACAAGCT TCCCGAGTTG
    TGGGCGCTAC TTAATTTCCT GCTGCCCTCG ATCTTTAAGT CCTGCTCCAC ATTTGAACAG
    TGGTTCAATG CGCCATTTGC CACCACTGGC GAGAAGGTCG AGCTAAACGA GGAGGAGACC
    ATCCTTATCA TTCGTCGTCT GCACAAGGTT CTTCGTCCTT TCCTGTTGCG TCGCCTCAAA
    AAGGAGGTAG AGCACCAGCT GCCCGACAAG GTCGAGTACA TCATCAAGTG CGACATGTCC
    GCGCTGCAAC GCGTTCTCTA CAAGCACATG CAGAGCAAGG GTGTCCTGCT CACCGATGGT
    TCCGAGAAGG GCAAGCACGG CAAGGGAGGC GCTAAGGCGC TGATGAACAC GATCGTACAG
    CTGCGCAAGC TGTGCAACCA TCCGTTTATG TTCCAGCACA TAGAAGAGAA GTACTGCGAT
    CACACTGGCG GCCACGGAGT TGTATCCGGA CCGGATCTGT ATCGTGTCTC TGGCAAATTC
    GAGCTGCTTG ATCGCATCCT GCCCAAGCTG AAGGCGACCA ATCATCGCGT GCTGCTCTTC
    TGTCAGATGA CCCAATGCAT GACCATCATC GAAGACTACC TGGGATGGCG CCAATTCGGC
    TACCTTCGAC TCGACGGTAC CACGAAGGCC GAGGATCGTG GCGAACTACT GCGAAAATTC
    AACGCCAAAG GCTCGGATGT ATTTGTCTTT TTGCTATCTA CCCGAGCTGG TGGTTTGGGT
    CTTAATCTGC AGACCGCCGA TACTGTAGTA ATCTTCGACT CCGATTGGAA TCCCCATCAG
    GATCTGCAAG CCCAGGATCG TGCCCATCGT ATTGGTCAAC GTAACGAGGT GCGCGTCCTG
    CGACTAATGA CAGTCAACTC GGTGGAAGAG CGCATCTTGG CAGCAGCCAG GTACAAGCTG
    AACATGGACG AGAAGGTCAT CCAGGCTGGT ATGTTCGATC AGAAGTCAAC GGGTAGCGAG
    CGACAGCAGT TCCTACAAAC GATTCTGCAC CAGGATGACA ACGAAGAGGA GGAGGAAAAT
    GAGGTGCCCG ATGACGAAAT GATCAACATG ATGATCGCCC GCAGCGAGGA GGAGATTGAA
    ATATTTAAGC GCATGGATGC TGAGCGCAAG AAGGAGGATG AGGAGATCCA TCCGGGTAGG
    GAACGTCTGA TCGATGAGTC CGAGCTACCC GACTGGTTGA CAAAGGATGA TGACGAAGTA
    GAGCGTTTCC ACTATCAGTA CGACGAGGAT ACCATTCTAG GTCGAGGCTC CCGGCAGCGA
    AAGGAAGTAG ACTACACCGA TAGTTTGACT GAAAAGGAGT GGCTGAAAGC CATCGACGAT
    GGCGCTGAGT TCGACGAGGA AGAGGAGGAA GACGATTCGA AGCGCAAGCG ACGAAAGCGT
    AAGAATCGGA AGGAAGAGTC CGACGATGAT TCGTTGATCC TAAAACGGCG ACGACGCCAA
    AATCTGGACA AGCGGTCCAA GAAGCAGATG CACAAGATTA TGAGTGCGGT TATCAAGCAC
    AACCAAGATG GACGAACCCT GTCCGAACCG TTCATGAAGT TGCCTTCGCG GCAGAGGTTG
    CCCGACTACT ACGAGATCAT TAAGCGGCCA GTTGATATTA AGAAAATCCT GCAGCGCATT
    GAGGACTGCA AGTATGCCGA TCTCAACGAG CTGGAGAAGG ACTTTATGCA GCTATGCCAG
    AATGCACAGA TCTACAACGA AGAGGCGTCT CTGATTTACC TGGACTCGAT TGCCCTGCAA
    AAGGTATTCG TTGGGGCCAG GCAGAGGATA ACAGCTGCAG CGGATGCAGC CGCAGTGGCA
    GCCGGAGATA ACACGGGTGA AGCGCACGGC AATGGAGGTT CGGATAATTC GGACAACGAC
    GACGACGATG GTGGCGATGA CGGCTCCGAC GACGAGGAGA TTGCAACCAC TTCAGCGGCG
    GCTGTCAAAA TGAAGCTGAA ACTGAACAAA TCTCTGGCTA GTGCGCCCGC TACGCCCACG
    CAATCATCGT CGAATGTGAG CAGCGGAGCG GCTACCACAT CCAAGAAGCA GACGCGTCGC
    AAGCGCTCCC AAAAGAAGTA CACTATTTCA GACGATGACG ACGATGACAT GGACTAGCTA
    CCGTTGGGAG TGATCTTCAA GCGCGGATCG TCGGATGTCG AAGGCATGGG CAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001261906.1
    CDS128..5104
    Translation

    Target ORF information:

    RefSeq Version NM_001274977.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster brahma (brm), transcript variant E, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001274977.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCCTCGC CCTCTCCGGC GAACAGTCCC ATGCCACCGC CACAGGCGCC CAGTCCGATG 
    GCCCCGCCCT CGCAGAGTCC CGCCCCCTCG CCGCACAGCC CGTATCCGCA CCAGCAGCCG
    GGTCCACTGC AAGGACCTCC GCCACCCGGA CATCCCGGTG CCTACGGACA TCCCATGCAG
    CACGGACCAC CCGGCCAGGG TCCACCCGGG CATCATATGC CACCGCACCA CCAGGGGATG
    ATTTTCTCAA AAGGTCCGCA CATGGGAATG CAGATGCCGC CGACGGGACC GAACATGTCC
    CCCTACCAAA CCCACGGAAT GCCACCCAAT GCACCCACAC AACCCTGCAT AGTGTCACCT
    GGTGGCCCAC CTGGAGGCCC GCCACCTCCG GAGCGCTCGA GCCAGGAGAA CCTGCACGCG
    CTTCAACGCG CCATTGATTC AATGGAGGAA AAGGGCTTGC AAGAGGATCC TCGCTACTCG
    CAGCTGCTGG CAATGCGAGC AACCTCGAAG CATCAGCACC TTAATGGCAA TCAGGTTAAC
    TTGTTGCGCA CACAGATCAC CGCCTATCGG TTGCTGGCGC GGAACAAGCC CATATCCATG
    CAGATGCAGC AGGCGTTGCA GGCCGCACAG CAACAGCCAC CGCCAGGACC TCCAATTGGG
    CCACCTGGAG CACCAGGCGG ACCGCCACCA GGGAGCCAGC ATGCAGGACA ACCGCCCGTG
    CCGCCGCAAC AACAGCAACA ACCGCCACCT TCAGCAGGAA CTCCACCCCA GTGCTCCACG
    CCGCCCGCAA GCAATCCTTA TGGACCGCCA GTTCCTGGCC AGAAAATGCA GGTAGCGCCA
    CCACCGCCAC ACATGCAACA GGGACAGCCA TTGCCACCAC AACCGCCACA AGTGGGTGGA
    CCCCCGCCTA TACAACAGCA GCAACCACCT CAGCAGCAGC AACAACAGTC GCAGCCCCCG
    CCACCGGAGC CACATCAGCA CCAGTTGCCA AACGGAGGCA AACCCCTGAG TATGGGGCCA
    TCTGGCGGGC AACCACTAAT ACCTTCATCA CCCATGCAGC CACAAGTGAG AGGGACACTG
    CCAGGAATGC CGCCAGGTAG TCAGGTTCCG CAACCAGGAG GAGGTCCTCA GCGACAGGTA
    CCACCTGCGG GCATGCCGAT GCCCAAGCCC AATCGCATTA CAACGGTGGC CAAACCCGTC
    GGTCTGGATC CCATAACATT GCTGCAGGAA AGGGAGAATA GGATAGCAGC CAGGATATCA
    TTGCGCATGC AGGAGTTGCA GCGACTGCCG GCCACGATGT CCGAGGATCT GCGTCTGCAA
    GCGGCTATCG AGTTGAGGGC CCTAAGGGTC TTGAACTTCC AGCGACAGCT GCGTATGGAG
    TTTGTTCAGT GCACGAGACG AGATACCACT TTGGAAACAG CGTTAAACAT TAAGCTCTAC
    AAGAGGACCA AGAGGCAGGG CCTGCGGGAG GCACGTGCCA CCGAGAAGCT AGAGAAGCAA
    CAGAAGCTAG AGGCTGAACG AAAGCGTCGC CAAAAGCATC TGGAGTTCTT GGCTGCTGTG
    CTTCAGCACG GCAAGGATCT AAGGGAGTTC CACAGAAATA ATAAGGCCCA GCTTGCAAGG
    ATGAACAAGG CCGTGATGAA TCACCACGCC AATGCCGAGC GTGAACAGAA GAAGGAACAA
    GAGCGCATCG AAAAGGAACG TATGCGACGT CTGATGGCCG AGGATGAGGA GGGTTACCGC
    AAGCTGATTG ATCAAAAGAA GGACAAGAGA TTGGCTTTCC TACTGTCGCA GACAGATGAG
    TATATTAGCA ACCTAACTCA GATGGTGAAA CAGCACAAGG ACGACCAGAT GAAGAAAAAG
    GAGGAGGAGG GCAAGCGGCT GATACAGTTC AAAAAGGAGC TGCTGATGAG CGGAGAGTAC
    ATTGGCATTG ATGAGGGCAG CATTGTTGCC GATATGCGGG TACATGTGGT GGAACAGTGC
    ACGGGTAAGA AACTCACCGG CGATGATGCA CCCATGCTGA AGCACTTGCA CCGCTGGCTG
    AATATGCATC CTGGCTGGGA TTGGATCGAC GACGAGGAGG ACAGCTGTGG GAGCAACGAC
    GATCATAAAC CCAAGGTGGA GGAGCAACCA ACGGCAACAG AAGATGCCAC CGACAAAGCG
    CAGGCGACGG GCAACGATGA GGATGCCAAG GATCTAATTA CCAAGGCCAA GGTGGAGGAC
    GATGAGTACA GGACGGAGGA ACAGACGTAC TACAGCATCG CTCATACCAT TCATGAAAAG
    GTTGTGGAGC AGGCCAGCAT CATGGTTAAT GGTACGCTCA AGGAATACCA GATCAAAGGC
    TTGGAGTGGT TGGTTTCGCT GTACAATAAC AATCTAAATG GTATTTTGGC CGATGAAATG
    GGTTTGGGTA AAACCATTCA AACCATTTCG CTGGTAACCT ACCTTATGGA TCGAAAGAAG
    GTTATGGGTC CTTACTTGAT TATTGTACCA CTCTCCACCC TGCCCAATTG GGTGCTGGAA
    TTCGAGAAGT GGGCGCCAGC CGTGGGTGTA GTCAGCTACA AGGGCAGTCC GCAGGGAAGG
    CGATTGCTGC AAAATCAAAT GAGAGCAACC AAGTTCAATG TGCTGTTAAC CACATATGAG
    TACGTGATTA AGGACAAGGC GGTGCTGGCC AAGATCCAGT GGAAATACAT GATTATCGAC
    GAGGGTCACC GCATGAAGAA CCATCACTGC AAACTCACCC AGGTGCTGAA CACCCACTAC
    ATTGCCCCCT ATCGGTTACT TCTCACGGGT ACACCTCTGC AAAACAAGCT TCCCGAGTTG
    TGGGCGCTAC TTAATTTCCT GCTGCCCTCG ATCTTTAAGT CCTGCTCCAC ATTTGAACAG
    TGGTTCAATG CGCCATTTGC CACCACTGGC GAGAAGGTCG AGCTAAACGA GGAGGAGACC
    ATCCTTATCA TTCGTCGTCT GCACAAGGTT CTTCGTCCTT TCCTGTTGCG TCGCCTCAAA
    AAGGAGGTAG AGCACCAGCT GCCCGACAAG GTCGAGTACA TCATCAAGTG CGACATGTCC
    GCGCTGCAAC GCGTTCTCTA CAAGCACATG CAGAGCAAGG GTGTCCTGCT CACCGATGGT
    TCCGAGAAGG GCAAGCACGG CAAGGGAGGC GCTAAGGCGC TGATGAACAC GATCGTACAG
    CTGCGCAAGC TGTGCAACCA TCCGTTTATG TTCCAGCACA TAGAAGAGAA GTACTGCGAT
    CACACTGGCG GCCACGGAGT TGTATCCGGA CCGGATCTGT ATCGTGTCTC TGGCAAATTC
    GAGCTGCTTG ATCGCATCCT GCCCAAGCTG AAGGCGACCA ATCATCGCGT GCTGCTCTTC
    TGTCAGATGA CCCAATGCAT GACCATCATC GAAGACTACC TGGGATGGCG CCAATTCGGC
    TACCTTCGAC TCGACGGTAC CACGAAGGCC GAGGATCGTG GCGAACTACT GCGAAAATTC
    AACGCCAAAG GCTCGGATGT ATTTGTCTTT TTGCTATCTA CCCGAGCTGG TGGTTTGGGT
    CTTAATCTGC AGACCGCCGA TACTGTAGTA ATCTTCGACT CCGATTGGAA TCCCCATCAG
    GATCTGCAAG CCCAGGATCG TGCCCATCGT ATTGGTCAAC GTAACGAGGT GCGCGTCCTG
    CGACTAATGA CAGTCAACTC GGTGGAAGAG CGCATCTTGG CAGCAGCCAG GTACAAGCTG
    AACATGGACG AGAAGGTCAT CCAGGCTGGT ATGTTCGATC AGAAGTCAAC GGGTAGCGAG
    CGACAGCAGT TCCTACAAAC GATTCTGCAC CAGGATGACA ACGAAGAGGA GGAGGAAAAT
    GAGGTGCCCG ATGACGAAAT GATCAACATG ATGATCGCCC GCAGCGAGGA GGAGATTGAA
    ATATTTAAGC GCATGGATGC TGAGCGCAAG AAGGAGGATG AGGAGATCCA TCCGGGTAGG
    GAACGTCTGA TCGATGAGTC CGAGCTACCC GACTGGTTGA CAAAGGATGA TGACGAAGTA
    GAGCGTTTCC ACTATCAGTA CGACGAGGAT ACCATTCTAG GTCGAGGCTC CCGGCAGCGA
    AAGGAAGTAG ACTACACCGA TAGTTTGACT GAAAAGGAGT GGCTGAAAGC CATCGACGAT
    GGCGCTGAGT TCGACGAGGA AGAGGAGGAA GACGATTCGA AGCGCAAGCG ACGAAAGCGT
    AAGAATCGGA AGGAAGAGTC CGACGATGAT TCGTTGATCC TAAAACGGCG ACGACGCCAA
    AATCTGGACA AGCGGTCCAA GAAGCAGATG CACAAGATTA TGAGTGCGGT TATCAAGCAC
    AACCAAGATG GACGAACCCT GTCCGAACCG TTCATGAAGT TGCCTTCGCG GCAGAGGTTG
    CCCGACTACT ACGAGATCAT TAAGCGGCCA GTTGATATTA AGAAAATCCT GCAGCGCATT
    GAGGACTGCA AGTATGCCGA TCTCAACGAG CTGGAGAAGG ACTTTATGCA GCTATGCCAG
    AATGCACAGA TCTACAACGA AGAGGCGTCT CTGATTTACC TGGACTCGAT TGCCCTGCAA
    AAGGTATTCG TTGGGGCCAG GCAGAGGATA ACAGCTGCAG CGGATGCAGC CGCAGTGGCA
    GCCGGAGATA ACACGGGTGA AGCGCACGGC AATGGAGGTT CGGATAATTC GGACAACGAC
    GACGACGATG GTGGCGATGA CGGCTCCGAC GACGAGGAGA TTGCAACCAC TTCAGCGGCG
    GCTGTCAAAA TGAAGCTGAA ACTGAACAAA TCTCTGGCTA GTGCGCCCGC TACGCCCACG
    CAATCATCGT CGAATGTGAG CAGCGGAGCG GCTACCACAT CCAAGAAGCA GACGCGTCGC
    AAGCGCTCCC AAAAGAAGTA CACTATTTCA GACGATGACG ACGATGACAT GGACTAGCTA
    CCGTTGGGAG TGATCTTCAA GCGCGGATCG TCGGATGTCG AAGGCATGGG CAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa20961
    Clone ID Related Accession (Same CDS sequence) NM_168640.2 , NM_080497.5
    Accession Version NM_168640.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4905bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product brahma, isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168640.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCCTCGC CCTCTCCGGC GAACAGTCCC ATGCCACCGC CACAGGCGCC CAGTCCGATG 
    GCCCCGCCCT CGCAGAGTCC CGCCCCCTCG CCGCACAGCC CGTATCCGCA CCAGCAGCCG
    GGTCCACTGC AAGGACCTCC GCCACCCGGA CATCCCGGTG CCTACGGACA TCCCATGCAG
    CACGGACCAC CCGGCCAGGG TCCACCCGGG CATCATATGC CACCGCACCA CCAGGGGATG
    AGTCCGCACA TGGGAATGCA GATGCCGCCG ACGGGACCGA ACATGTCCCC CTACCAAACC
    CACGGAATGC CACCCAATGC ACCCACACAA CCCTGCATAG TGTCACCTGG TGGCCCACCT
    GGAGGCCCGC CACCTCCGGA GCGCTCGAGC CAGGAGAACC TGCACGCGCT TCAACGCGCC
    ATTGATTCAA TGGAGGAAAA GGGCTTGCAA GAGGATCCTC GCTACTCGCA GCTGCTGGCA
    ATGCGAGCAA CCTCGAAGCA TCAGCACCTT AATGGCAATC AGGTTAACTT GTTGCGCACA
    CAGATCACCG CCTATCGGTT GCTGGCGCGG AACAAGCCCA TATCCATGCA GATGCAGCAG
    GCGTTGCAGG CCGCACAGCA ACAGCCACCG CCAGGACCTC CAATTGGGCC ACCTGGAGCA
    CCAGGCGGAC CGCCACCAGG GAGCCAGCAT GCAGGACAAC CGCCCGTGCC GCCGCAACAA
    CAGCAACAAC CGCCACCTTC AGCAGGAACT CCACCCCAGT GCTCCACGCC GCCCGCAAGC
    AATCCTTATG GACCGCCAGT TCCTGGCCAG AAAATGCAGG TAGCGCCACC ACCGCCACAC
    ATGCAACAGG GACAGCCATT GCCACCACAA CCGCCACAAG TGGGTGGACC CCCGCCTATA
    CAACAGCAGC AACCACCTCA GCAGCAGCAA CAACAGTCGC AGCCCCCGCC ACCGGAGCCA
    CATCAGCACC AGTTGCCAAA CGGAGGCAAA CCCCTGAGTA TGGGGCCATC TGGCGGGCAA
    CCACTAATAC CTTCATCACC CATGCAGCCA CAAGTGAGAG GGACACTGCC AGGAATGCCG
    CCAGGTAGTC AGGTTCCGCA ACCAGGAGGA GGTCCTCAGC GACAGGTACC ACCTGCGGGC
    ATGCCGATGC CCAAGCCCAA TCGCATTACA ACGGTGGCCA AACCCGTCGG TCTGGATCCC
    ATAACATTGC TGCAGGAAAG GGAGAATAGG ATAGCAGCCA GGATATCATT GCGCATGCAG
    GAGTTGCAGC GACTGCCGGC CACGATGTCC GAGGATCTGC GTCTGCAAGC GGCTATCGAG
    TTGAGGGCCC TAAGGGTCTT GAACTTCCAG CGACAGCTGC GTATGGAGTT TGTTCAGTGC
    ACGAGACGAG ATACCACTTT GGAAACAGCG TTAAACATTA AGCTCTACAA GAGGACCAAG
    AGGCAGGGCC TGCGGGAGGC ACGTGCCACC GAGAAGCTAG AGAAGCAACA GAAGCTAGAG
    GCTGAACGAA AGCGTCGCCA AAAGCATCTG GAGTTCTTGG CTGCTGTGCT TCAGCACGGC
    AAGGATCTAA GGGAGTTCCA CAGAAATAAT AAGGCCCAGC TTGCAAGGAT GAACAAGGCC
    GTGATGAATC ACCACGCCAA TGCCGAGCGT GAACAGAAGA AGGAACAAGA GCGCATCGAA
    AAGGAACGTA TGCGACGTCT GATGGCCGAG GATGAGGAGG GTTACCGCAA GCTGATTGAT
    CAAAAGAAGG ACAAGAGATT GGCTTTCCTA CTGTCGCAGA CAGATGAGTA TATTAGCAAC
    CTAACTCAGA TGGTGAAACA GCACAAGGAC GACCAGATGA AGAAAAAGGA GGAGGAGGGC
    AAGCGGCTGA TACAGTTCAA AAAGGAGCTG CTGATGAGCG GAGAGTACAT TGGCATTGAT
    GAGGGCAGCA TTGTTGCCGA TATGCGGGTA CATGTGGTGG AACAGTGCAC GGGTAAGAAA
    CTCACCGGCG ATGATGCACC CATGCTGAAG CACTTGCACC GCTGGCTGAA TATGCATCCT
    GGCTGGGATT GGATCGACGA CGAGGAGGAC AGCTGTGGGA GCAACGACGA TCATAAACCC
    AAGGTGGAGG AGCAACCAAC GGCAACAGAA GATGCCACCG ACAAAGCGCA GGCGACGGGC
    AACGATGAGG ATGCCAAGGA TCTAATTACC AAGGCCAAGG TGGAGGACGA TGAGTACAGG
    ACGGAGGAAC AGACGTACTA CAGCATCGCT CATACCATTC ATGAAAAGGT TGTGGAGCAG
    GCCAGCATCA TGGTTAATGG TACGCTCAAG GAATACCAGA TCAAAGGCTT GGAGTGGTTG
    GTTTCGCTGT ACAATAACAA TCTAAATGGT ATTTTGGCCG ATGAAATGGG TTTGGGTAAA
    ACCATTCAAA CCATTTCGCT GGTAACCTAC CTTATGGATC GAAAGAAGGT TATGGGTCCT
    TACTTGATTA TTGTACCACT CTCCACCCTG CCCAATTGGG TGCTGGAATT CGAGAAGTGG
    GCGCCAGCCG TGGGTGTAGT CAGCTACAAG GGCAGTCCGC AGGGAAGGCG ATTGCTGCAA
    AATCAAATGA GAGCAACCAA GTTCAATGTG CTGTTAACCA CATATGAGTA CGTGATTAAG
    GACAAGGCGG TGCTGGCCAA GATCCAGTGG AAATACATGA TTATCGACGA GGGTCACCGC
    ATGAAGAACC ATCACTGCAA ACTCACCCAG GTGCTGAACA CCCACTACAT TGCCCCCTAT
    CGGTTACTTC TCACGGGTAC ACCTCTGCAA AACAAGCTTC CCGAGTTGTG GGCGCTACTT
    AATTTCCTGC TGCCCTCGAT CTTTAAGTCC TGCTCCACAT TTGAACAGTG GTTCAATGCG
    CCATTTGCCA CCACTGGCGA GAAGGTCGAG CTAAACGAGG AGGAGACCAT CCTTATCATT
    CGTCGTCTGC ACAAGGTTCT TCGTCCTTTC CTGTTGCGTC GCCTCAAAAA GGAGGTAGAG
    CACCAGCTGC CCGACAAGGT CGAGTACATC ATCAAGTGCG ACATGTCCGC GCTGCAACGC
    GTTCTCTACA AGCACATGCA GAGCAAGGGT GTCCTGCTCA CCGATGGTTC CGAGAAGGGC
    AAGCACGGCA AGGGAGGCGC TAAGGCGCTG ATGAACACGA TCGTACAGCT GCGCAAGCTG
    TGCAACCATC CGTTTATGTT CCAGCACATA GAAGAGAAGT ACTGCGATCA CACTGGCGGC
    CACGGAGTTG TATCCGGACC GGATCTGTAT CGTGTCTCTG GCAAATTCGA GCTGCTTGAT
    CGCATCCTGC CCAAGCTGAA GGCGACCAAT CATCGCGTGC TGCTCTTCTG TCAGATGACC
    CAATGCATGA CCATCATCGA AGACTACCTG GGATGGCGCC AATTCGGCTA CCTTCGACTC
    GACGGTACCA CGAAGGCCGA GGATCGTGGC GAACTACTGC GAAAATTCAA CGCCAAAGGC
    TCGGATGTAT TTGTCTTTTT GCTATCTACC CGAGCTGGTG GTTTGGGTCT TAATCTGCAG
    ACCGCCGATA CTGTAGTAAT CTTCGACTCC GATTGGAATC CCCATCAGGA TCTGCAAGCC
    CAGGATCGTG CCCATCGTAT TGGTCAACGT AACGAGGTGC GCGTCCTGCG ACTAATGACA
    GTCAACTCGG TGGAAGAGCG CATCTTGGCA GCAGCCAGGT ACAAGCTGAA CATGGACGAG
    AAGGTCATCC AGGCTGGTAT GTTCGATCAG AAGTCAACGG GTAGCGAGCG ACAGCAGTTC
    CTACAAACGA TTCTGCACCA GGATGACAAC GAAGAGGAGG AGGAAAATGA GGTGCCCGAT
    GACGAAATGA TCAACATGAT GATCGCCCGC AGCGAGGAGG AGATTGAAAT ATTTAAGCGC
    ATGGATGCTG AGCGCAAGAA GGAGGATGAG GAGATCCATC CGGGTAGGGA ACGTCTGATC
    GATGAGTCCG AGCTACCCGA CTGGTTGACA AAGGATGATG ACGAAGTAGA GCGTTTCCAC
    TATCAGTACG ACGAGGATAC CATTCTAGGT CGAGGCTCCC GGCAGCGAAA GGAAGTAGAC
    TACACCGATA GTTTGACTGA AAAGGAGTGG CTGAAAGCCA TCGACGATGG CGCTGAGTTC
    GACGAGGAAG AGGAGGAAGA CGATTCGAAG CGCAAGCGAC GAAAGCGTAA GAATCGGAAG
    GAAGAGTCCG ACGATGATTC GTTGATCCTA AAACGGCGAC GACGCCAAAA TCTGGACAAG
    CGGTCCAAGA AGCAGATGCA CAAGATTATG AGTGCGGTTA TCAAGCACAA CCAAGATGGA
    CGAACCCTGT CCGAACCGTT CATGAAGTTG CCTTCGCGGC AGAGGTTGCC CGACTACTAC
    GAGATCATTA AGCGGCCAGT TGATATTAAG AAAATCCTGC AGCGCATTGA GGACTGCAAG
    TATGCCGATC TCAACGAGCT GGAGAAGGAC TTTATGCAGC TATGCCAGAA TGCACAGATC
    TACAACGAAG AGGCGTCTCT GATTTACCTG GACTCGATTG CCCTGCAAAA GGTATTCGTT
    GGGGCCAGGC AGAGGATAAC AGCTGCAGCG GATGCAGCCG CAGTGGCAGC CGGAGATAAC
    ACGGGTGAAG CGCACGGCAA TGGAGGTTCG GATAATTCGG ACAACGACGA CGACGATGGT
    GGCGATGACG GCTCCGACGA CGAGGAGATT GCAACCACTT CAGCGGCGGC TGTCAAAATG
    AAGCTGAAAC TGAACAAATC TCTGGCTAGT GCGCCCGCTA CGCCCACGCA ATCATCGTCG
    AATGTGAGCA GCGGAGCGGC TACCACATCC AAGAAGCAGA CGCGTCGCAA GCGCTCCCAA
    AAGAAGTACA CTATTTCAGA CGATGACGAC GATGACATGG ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_730088.1
    CDS123..5027
    Translation

    Target ORF information:

    RefSeq Version NM_168640.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster brahma (brm), transcript variant D, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_168640.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCCTCGC CCTCTCCGGC GAACAGTCCC ATGCCACCGC CACAGGCGCC CAGTCCGATG 
    GCCCCGCCCT CGCAGAGTCC CGCCCCCTCG CCGCACAGCC CGTATCCGCA CCAGCAGCCG
    GGTCCACTGC AAGGACCTCC GCCACCCGGA CATCCCGGTG CCTACGGACA TCCCATGCAG
    CACGGACCAC CCGGCCAGGG TCCACCCGGG CATCATATGC CACCGCACCA CCAGGGGATG
    AGTCCGCACA TGGGAATGCA GATGCCGCCG ACGGGACCGA ACATGTCCCC CTACCAAACC
    CACGGAATGC CACCCAATGC ACCCACACAA CCCTGCATAG TGTCACCTGG TGGCCCACCT
    GGAGGCCCGC CACCTCCGGA GCGCTCGAGC CAGGAGAACC TGCACGCGCT TCAACGCGCC
    ATTGATTCAA TGGAGGAAAA GGGCTTGCAA GAGGATCCTC GCTACTCGCA GCTGCTGGCA
    ATGCGAGCAA CCTCGAAGCA TCAGCACCTT AATGGCAATC AGGTTAACTT GTTGCGCACA
    CAGATCACCG CCTATCGGTT GCTGGCGCGG AACAAGCCCA TATCCATGCA GATGCAGCAG
    GCGTTGCAGG CCGCACAGCA ACAGCCACCG CCAGGACCTC CAATTGGGCC ACCTGGAGCA
    CCAGGCGGAC CGCCACCAGG GAGCCAGCAT GCAGGACAAC CGCCCGTGCC GCCGCAACAA
    CAGCAACAAC CGCCACCTTC AGCAGGAACT CCACCCCAGT GCTCCACGCC GCCCGCAAGC
    AATCCTTATG GACCGCCAGT TCCTGGCCAG AAAATGCAGG TAGCGCCACC ACCGCCACAC
    ATGCAACAGG GACAGCCATT GCCACCACAA CCGCCACAAG TGGGTGGACC CCCGCCTATA
    CAACAGCAGC AACCACCTCA GCAGCAGCAA CAACAGTCGC AGCCCCCGCC ACCGGAGCCA
    CATCAGCACC AGTTGCCAAA CGGAGGCAAA CCCCTGAGTA TGGGGCCATC TGGCGGGCAA
    CCACTAATAC CTTCATCACC CATGCAGCCA CAAGTGAGAG GGACACTGCC AGGAATGCCG
    CCAGGTAGTC AGGTTCCGCA ACCAGGAGGA GGTCCTCAGC GACAGGTACC ACCTGCGGGC
    ATGCCGATGC CCAAGCCCAA TCGCATTACA ACGGTGGCCA AACCCGTCGG TCTGGATCCC
    ATAACATTGC TGCAGGAAAG GGAGAATAGG ATAGCAGCCA GGATATCATT GCGCATGCAG
    GAGTTGCAGC GACTGCCGGC CACGATGTCC GAGGATCTGC GTCTGCAAGC GGCTATCGAG
    TTGAGGGCCC TAAGGGTCTT GAACTTCCAG CGACAGCTGC GTATGGAGTT TGTTCAGTGC
    ACGAGACGAG ATACCACTTT GGAAACAGCG TTAAACATTA AGCTCTACAA GAGGACCAAG
    AGGCAGGGCC TGCGGGAGGC ACGTGCCACC GAGAAGCTAG AGAAGCAACA GAAGCTAGAG
    GCTGAACGAA AGCGTCGCCA AAAGCATCTG GAGTTCTTGG CTGCTGTGCT TCAGCACGGC
    AAGGATCTAA GGGAGTTCCA CAGAAATAAT AAGGCCCAGC TTGCAAGGAT GAACAAGGCC
    GTGATGAATC ACCACGCCAA TGCCGAGCGT GAACAGAAGA AGGAACAAGA GCGCATCGAA
    AAGGAACGTA TGCGACGTCT GATGGCCGAG GATGAGGAGG GTTACCGCAA GCTGATTGAT
    CAAAAGAAGG ACAAGAGATT GGCTTTCCTA CTGTCGCAGA CAGATGAGTA TATTAGCAAC
    CTAACTCAGA TGGTGAAACA GCACAAGGAC GACCAGATGA AGAAAAAGGA GGAGGAGGGC
    AAGCGGCTGA TACAGTTCAA AAAGGAGCTG CTGATGAGCG GAGAGTACAT TGGCATTGAT
    GAGGGCAGCA TTGTTGCCGA TATGCGGGTA CATGTGGTGG AACAGTGCAC GGGTAAGAAA
    CTCACCGGCG ATGATGCACC CATGCTGAAG CACTTGCACC GCTGGCTGAA TATGCATCCT
    GGCTGGGATT GGATCGACGA CGAGGAGGAC AGCTGTGGGA GCAACGACGA TCATAAACCC
    AAGGTGGAGG AGCAACCAAC GGCAACAGAA GATGCCACCG ACAAAGCGCA GGCGACGGGC
    AACGATGAGG ATGCCAAGGA TCTAATTACC AAGGCCAAGG TGGAGGACGA TGAGTACAGG
    ACGGAGGAAC AGACGTACTA CAGCATCGCT CATACCATTC ATGAAAAGGT TGTGGAGCAG
    GCCAGCATCA TGGTTAATGG TACGCTCAAG GAATACCAGA TCAAAGGCTT GGAGTGGTTG
    GTTTCGCTGT ACAATAACAA TCTAAATGGT ATTTTGGCCG ATGAAATGGG TTTGGGTAAA
    ACCATTCAAA CCATTTCGCT GGTAACCTAC CTTATGGATC GAAAGAAGGT TATGGGTCCT
    TACTTGATTA TTGTACCACT CTCCACCCTG CCCAATTGGG TGCTGGAATT CGAGAAGTGG
    GCGCCAGCCG TGGGTGTAGT CAGCTACAAG GGCAGTCCGC AGGGAAGGCG ATTGCTGCAA
    AATCAAATGA GAGCAACCAA GTTCAATGTG CTGTTAACCA CATATGAGTA CGTGATTAAG
    GACAAGGCGG TGCTGGCCAA GATCCAGTGG AAATACATGA TTATCGACGA GGGTCACCGC
    ATGAAGAACC ATCACTGCAA ACTCACCCAG GTGCTGAACA CCCACTACAT TGCCCCCTAT
    CGGTTACTTC TCACGGGTAC ACCTCTGCAA AACAAGCTTC CCGAGTTGTG GGCGCTACTT
    AATTTCCTGC TGCCCTCGAT CTTTAAGTCC TGCTCCACAT TTGAACAGTG GTTCAATGCG
    CCATTTGCCA CCACTGGCGA GAAGGTCGAG CTAAACGAGG AGGAGACCAT CCTTATCATT
    CGTCGTCTGC ACAAGGTTCT TCGTCCTTTC CTGTTGCGTC GCCTCAAAAA GGAGGTAGAG
    CACCAGCTGC CCGACAAGGT CGAGTACATC ATCAAGTGCG ACATGTCCGC GCTGCAACGC
    GTTCTCTACA AGCACATGCA GAGCAAGGGT GTCCTGCTCA CCGATGGTTC CGAGAAGGGC
    AAGCACGGCA AGGGAGGCGC TAAGGCGCTG ATGAACACGA TCGTACAGCT GCGCAAGCTG
    TGCAACCATC CGTTTATGTT CCAGCACATA GAAGAGAAGT ACTGCGATCA CACTGGCGGC
    CACGGAGTTG TATCCGGACC GGATCTGTAT CGTGTCTCTG GCAAATTCGA GCTGCTTGAT
    CGCATCCTGC CCAAGCTGAA GGCGACCAAT CATCGCGTGC TGCTCTTCTG TCAGATGACC
    CAATGCATGA CCATCATCGA AGACTACCTG GGATGGCGCC AATTCGGCTA CCTTCGACTC
    GACGGTACCA CGAAGGCCGA GGATCGTGGC GAACTACTGC GAAAATTCAA CGCCAAAGGC
    TCGGATGTAT TTGTCTTTTT GCTATCTACC CGAGCTGGTG GTTTGGGTCT TAATCTGCAG
    ACCGCCGATA CTGTAGTAAT CTTCGACTCC GATTGGAATC CCCATCAGGA TCTGCAAGCC
    CAGGATCGTG CCCATCGTAT TGGTCAACGT AACGAGGTGC GCGTCCTGCG ACTAATGACA
    GTCAACTCGG TGGAAGAGCG CATCTTGGCA GCAGCCAGGT ACAAGCTGAA CATGGACGAG
    AAGGTCATCC AGGCTGGTAT GTTCGATCAG AAGTCAACGG GTAGCGAGCG ACAGCAGTTC
    CTACAAACGA TTCTGCACCA GGATGACAAC GAAGAGGAGG AGGAAAATGA GGTGCCCGAT
    GACGAAATGA TCAACATGAT GATCGCCCGC AGCGAGGAGG AGATTGAAAT ATTTAAGCGC
    ATGGATGCTG AGCGCAAGAA GGAGGATGAG GAGATCCATC CGGGTAGGGA ACGTCTGATC
    GATGAGTCCG AGCTACCCGA CTGGTTGACA AAGGATGATG ACGAAGTAGA GCGTTTCCAC
    TATCAGTACG ACGAGGATAC CATTCTAGGT CGAGGCTCCC GGCAGCGAAA GGAAGTAGAC
    TACACCGATA GTTTGACTGA AAAGGAGTGG CTGAAAGCCA TCGACGATGG CGCTGAGTTC
    GACGAGGAAG AGGAGGAAGA CGATTCGAAG CGCAAGCGAC GAAAGCGTAA GAATCGGAAG
    GAAGAGTCCG ACGATGATTC GTTGATCCTA AAACGGCGAC GACGCCAAAA TCTGGACAAG
    CGGTCCAAGA AGCAGATGCA CAAGATTATG AGTGCGGTTA TCAAGCACAA CCAAGATGGA
    CGAACCCTGT CCGAACCGTT CATGAAGTTG CCTTCGCGGC AGAGGTTGCC CGACTACTAC
    GAGATCATTA AGCGGCCAGT TGATATTAAG AAAATCCTGC AGCGCATTGA GGACTGCAAG
    TATGCCGATC TCAACGAGCT GGAGAAGGAC TTTATGCAGC TATGCCAGAA TGCACAGATC
    TACAACGAAG AGGCGTCTCT GATTTACCTG GACTCGATTG CCCTGCAAAA GGTATTCGTT
    GGGGCCAGGC AGAGGATAAC AGCTGCAGCG GATGCAGCCG CAGTGGCAGC CGGAGATAAC
    ACGGGTGAAG CGCACGGCAA TGGAGGTTCG GATAATTCGG ACAACGACGA CGACGATGGT
    GGCGATGACG GCTCCGACGA CGAGGAGATT GCAACCACTT CAGCGGCGGC TGTCAAAATG
    AAGCTGAAAC TGAACAAATC TCTGGCTAGT GCGCCCGCTA CGCCCACGCA ATCATCGTCG
    AATGTGAGCA GCGGAGCGGC TACCACATCC AAGAAGCAGA CGCGTCGCAA GCGCTCCCAA
    AAGAAGTACA CTATTTCAGA CGATGACGAC GATGACATGG ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa20961
    Clone ID Related Accession (Same CDS sequence) NM_168640.2 , NM_080497.5
    Accession Version NM_080497.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4905bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product brahma, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_080497.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCCTCGC CCTCTCCGGC GAACAGTCCC ATGCCACCGC CACAGGCGCC CAGTCCGATG 
    GCCCCGCCCT CGCAGAGTCC CGCCCCCTCG CCGCACAGCC CGTATCCGCA CCAGCAGCCG
    GGTCCACTGC AAGGACCTCC GCCACCCGGA CATCCCGGTG CCTACGGACA TCCCATGCAG
    CACGGACCAC CCGGCCAGGG TCCACCCGGG CATCATATGC CACCGCACCA CCAGGGGATG
    AGTCCGCACA TGGGAATGCA GATGCCGCCG ACGGGACCGA ACATGTCCCC CTACCAAACC
    CACGGAATGC CACCCAATGC ACCCACACAA CCCTGCATAG TGTCACCTGG TGGCCCACCT
    GGAGGCCCGC CACCTCCGGA GCGCTCGAGC CAGGAGAACC TGCACGCGCT TCAACGCGCC
    ATTGATTCAA TGGAGGAAAA GGGCTTGCAA GAGGATCCTC GCTACTCGCA GCTGCTGGCA
    ATGCGAGCAA CCTCGAAGCA TCAGCACCTT AATGGCAATC AGGTTAACTT GTTGCGCACA
    CAGATCACCG CCTATCGGTT GCTGGCGCGG AACAAGCCCA TATCCATGCA GATGCAGCAG
    GCGTTGCAGG CCGCACAGCA ACAGCCACCG CCAGGACCTC CAATTGGGCC ACCTGGAGCA
    CCAGGCGGAC CGCCACCAGG GAGCCAGCAT GCAGGACAAC CGCCCGTGCC GCCGCAACAA
    CAGCAACAAC CGCCACCTTC AGCAGGAACT CCACCCCAGT GCTCCACGCC GCCCGCAAGC
    AATCCTTATG GACCGCCAGT TCCTGGCCAG AAAATGCAGG TAGCGCCACC ACCGCCACAC
    ATGCAACAGG GACAGCCATT GCCACCACAA CCGCCACAAG TGGGTGGACC CCCGCCTATA
    CAACAGCAGC AACCACCTCA GCAGCAGCAA CAACAGTCGC AGCCCCCGCC ACCGGAGCCA
    CATCAGCACC AGTTGCCAAA CGGAGGCAAA CCCCTGAGTA TGGGGCCATC TGGCGGGCAA
    CCACTAATAC CTTCATCACC CATGCAGCCA CAAGTGAGAG GGACACTGCC AGGAATGCCG
    CCAGGTAGTC AGGTTCCGCA ACCAGGAGGA GGTCCTCAGC GACAGGTACC ACCTGCGGGC
    ATGCCGATGC CCAAGCCCAA TCGCATTACA ACGGTGGCCA AACCCGTCGG TCTGGATCCC
    ATAACATTGC TGCAGGAAAG GGAGAATAGG ATAGCAGCCA GGATATCATT GCGCATGCAG
    GAGTTGCAGC GACTGCCGGC CACGATGTCC GAGGATCTGC GTCTGCAAGC GGCTATCGAG
    TTGAGGGCCC TAAGGGTCTT GAACTTCCAG CGACAGCTGC GTATGGAGTT TGTTCAGTGC
    ACGAGACGAG ATACCACTTT GGAAACAGCG TTAAACATTA AGCTCTACAA GAGGACCAAG
    AGGCAGGGCC TGCGGGAGGC ACGTGCCACC GAGAAGCTAG AGAAGCAACA GAAGCTAGAG
    GCTGAACGAA AGCGTCGCCA AAAGCATCTG GAGTTCTTGG CTGCTGTGCT TCAGCACGGC
    AAGGATCTAA GGGAGTTCCA CAGAAATAAT AAGGCCCAGC TTGCAAGGAT GAACAAGGCC
    GTGATGAATC ACCACGCCAA TGCCGAGCGT GAACAGAAGA AGGAACAAGA GCGCATCGAA
    AAGGAACGTA TGCGACGTCT GATGGCCGAG GATGAGGAGG GTTACCGCAA GCTGATTGAT
    CAAAAGAAGG ACAAGAGATT GGCTTTCCTA CTGTCGCAGA CAGATGAGTA TATTAGCAAC
    CTAACTCAGA TGGTGAAACA GCACAAGGAC GACCAGATGA AGAAAAAGGA GGAGGAGGGC
    AAGCGGCTGA TACAGTTCAA AAAGGAGCTG CTGATGAGCG GAGAGTACAT TGGCATTGAT
    GAGGGCAGCA TTGTTGCCGA TATGCGGGTA CATGTGGTGG AACAGTGCAC GGGTAAGAAA
    CTCACCGGCG ATGATGCACC CATGCTGAAG CACTTGCACC GCTGGCTGAA TATGCATCCT
    GGCTGGGATT GGATCGACGA CGAGGAGGAC AGCTGTGGGA GCAACGACGA TCATAAACCC
    AAGGTGGAGG AGCAACCAAC GGCAACAGAA GATGCCACCG ACAAAGCGCA GGCGACGGGC
    AACGATGAGG ATGCCAAGGA TCTAATTACC AAGGCCAAGG TGGAGGACGA TGAGTACAGG
    ACGGAGGAAC AGACGTACTA CAGCATCGCT CATACCATTC ATGAAAAGGT TGTGGAGCAG
    GCCAGCATCA TGGTTAATGG TACGCTCAAG GAATACCAGA TCAAAGGCTT GGAGTGGTTG
    GTTTCGCTGT ACAATAACAA TCTAAATGGT ATTTTGGCCG ATGAAATGGG TTTGGGTAAA
    ACCATTCAAA CCATTTCGCT GGTAACCTAC CTTATGGATC GAAAGAAGGT TATGGGTCCT
    TACTTGATTA TTGTACCACT CTCCACCCTG CCCAATTGGG TGCTGGAATT CGAGAAGTGG
    GCGCCAGCCG TGGGTGTAGT CAGCTACAAG GGCAGTCCGC AGGGAAGGCG ATTGCTGCAA
    AATCAAATGA GAGCAACCAA GTTCAATGTG CTGTTAACCA CATATGAGTA CGTGATTAAG
    GACAAGGCGG TGCTGGCCAA GATCCAGTGG AAATACATGA TTATCGACGA GGGTCACCGC
    ATGAAGAACC ATCACTGCAA ACTCACCCAG GTGCTGAACA CCCACTACAT TGCCCCCTAT
    CGGTTACTTC TCACGGGTAC ACCTCTGCAA AACAAGCTTC CCGAGTTGTG GGCGCTACTT
    AATTTCCTGC TGCCCTCGAT CTTTAAGTCC TGCTCCACAT TTGAACAGTG GTTCAATGCG
    CCATTTGCCA CCACTGGCGA GAAGGTCGAG CTAAACGAGG AGGAGACCAT CCTTATCATT
    CGTCGTCTGC ACAAGGTTCT TCGTCCTTTC CTGTTGCGTC GCCTCAAAAA GGAGGTAGAG
    CACCAGCTGC CCGACAAGGT CGAGTACATC ATCAAGTGCG ACATGTCCGC GCTGCAACGC
    GTTCTCTACA AGCACATGCA GAGCAAGGGT GTCCTGCTCA CCGATGGTTC CGAGAAGGGC
    AAGCACGGCA AGGGAGGCGC TAAGGCGCTG ATGAACACGA TCGTACAGCT GCGCAAGCTG
    TGCAACCATC CGTTTATGTT CCAGCACATA GAAGAGAAGT ACTGCGATCA CACTGGCGGC
    CACGGAGTTG TATCCGGACC GGATCTGTAT CGTGTCTCTG GCAAATTCGA GCTGCTTGAT
    CGCATCCTGC CCAAGCTGAA GGCGACCAAT CATCGCGTGC TGCTCTTCTG TCAGATGACC
    CAATGCATGA CCATCATCGA AGACTACCTG GGATGGCGCC AATTCGGCTA CCTTCGACTC
    GACGGTACCA CGAAGGCCGA GGATCGTGGC GAACTACTGC GAAAATTCAA CGCCAAAGGC
    TCGGATGTAT TTGTCTTTTT GCTATCTACC CGAGCTGGTG GTTTGGGTCT TAATCTGCAG
    ACCGCCGATA CTGTAGTAAT CTTCGACTCC GATTGGAATC CCCATCAGGA TCTGCAAGCC
    CAGGATCGTG CCCATCGTAT TGGTCAACGT AACGAGGTGC GCGTCCTGCG ACTAATGACA
    GTCAACTCGG TGGAAGAGCG CATCTTGGCA GCAGCCAGGT ACAAGCTGAA CATGGACGAG
    AAGGTCATCC AGGCTGGTAT GTTCGATCAG AAGTCAACGG GTAGCGAGCG ACAGCAGTTC
    CTACAAACGA TTCTGCACCA GGATGACAAC GAAGAGGAGG AGGAAAATGA GGTGCCCGAT
    GACGAAATGA TCAACATGAT GATCGCCCGC AGCGAGGAGG AGATTGAAAT ATTTAAGCGC
    ATGGATGCTG AGCGCAAGAA GGAGGATGAG GAGATCCATC CGGGTAGGGA ACGTCTGATC
    GATGAGTCCG AGCTACCCGA CTGGTTGACA AAGGATGATG ACGAAGTAGA GCGTTTCCAC
    TATCAGTACG ACGAGGATAC CATTCTAGGT CGAGGCTCCC GGCAGCGAAA GGAAGTAGAC
    TACACCGATA GTTTGACTGA AAAGGAGTGG CTGAAAGCCA TCGACGATGG CGCTGAGTTC
    GACGAGGAAG AGGAGGAAGA CGATTCGAAG CGCAAGCGAC GAAAGCGTAA GAATCGGAAG
    GAAGAGTCCG ACGATGATTC GTTGATCCTA AAACGGCGAC GACGCCAAAA TCTGGACAAG
    CGGTCCAAGA AGCAGATGCA CAAGATTATG AGTGCGGTTA TCAAGCACAA CCAAGATGGA
    CGAACCCTGT CCGAACCGTT CATGAAGTTG CCTTCGCGGC AGAGGTTGCC CGACTACTAC
    GAGATCATTA AGCGGCCAGT TGATATTAAG AAAATCCTGC AGCGCATTGA GGACTGCAAG
    TATGCCGATC TCAACGAGCT GGAGAAGGAC TTTATGCAGC TATGCCAGAA TGCACAGATC
    TACAACGAAG AGGCGTCTCT GATTTACCTG GACTCGATTG CCCTGCAAAA GGTATTCGTT
    GGGGCCAGGC AGAGGATAAC AGCTGCAGCG GATGCAGCCG CAGTGGCAGC CGGAGATAAC
    ACGGGTGAAG CGCACGGCAA TGGAGGTTCG GATAATTCGG ACAACGACGA CGACGATGGT
    GGCGATGACG GCTCCGACGA CGAGGAGATT GCAACCACTT CAGCGGCGGC TGTCAAAATG
    AAGCTGAAAC TGAACAAATC TCTGGCTAGT GCGCCCGCTA CGCCCACGCA ATCATCGTCG
    AATGTGAGCA GCGGAGCGGC TACCACATCC AAGAAGCAGA CGCGTCGCAA GCGCTCCCAA
    AAGAAGTACA CTATTTCAGA CGATGACGAC GATGACATGG ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_536745.4
    CDS128..5032
    Translation

    Target ORF information:

    RefSeq Version NM_080497.5
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster brahma (brm), transcript variant C, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_080497.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCCTCGC CCTCTCCGGC GAACAGTCCC ATGCCACCGC CACAGGCGCC CAGTCCGATG 
    GCCCCGCCCT CGCAGAGTCC CGCCCCCTCG CCGCACAGCC CGTATCCGCA CCAGCAGCCG
    GGTCCACTGC AAGGACCTCC GCCACCCGGA CATCCCGGTG CCTACGGACA TCCCATGCAG
    CACGGACCAC CCGGCCAGGG TCCACCCGGG CATCATATGC CACCGCACCA CCAGGGGATG
    AGTCCGCACA TGGGAATGCA GATGCCGCCG ACGGGACCGA ACATGTCCCC CTACCAAACC
    CACGGAATGC CACCCAATGC ACCCACACAA CCCTGCATAG TGTCACCTGG TGGCCCACCT
    GGAGGCCCGC CACCTCCGGA GCGCTCGAGC CAGGAGAACC TGCACGCGCT TCAACGCGCC
    ATTGATTCAA TGGAGGAAAA GGGCTTGCAA GAGGATCCTC GCTACTCGCA GCTGCTGGCA
    ATGCGAGCAA CCTCGAAGCA TCAGCACCTT AATGGCAATC AGGTTAACTT GTTGCGCACA
    CAGATCACCG CCTATCGGTT GCTGGCGCGG AACAAGCCCA TATCCATGCA GATGCAGCAG
    GCGTTGCAGG CCGCACAGCA ACAGCCACCG CCAGGACCTC CAATTGGGCC ACCTGGAGCA
    CCAGGCGGAC CGCCACCAGG GAGCCAGCAT GCAGGACAAC CGCCCGTGCC GCCGCAACAA
    CAGCAACAAC CGCCACCTTC AGCAGGAACT CCACCCCAGT GCTCCACGCC GCCCGCAAGC
    AATCCTTATG GACCGCCAGT TCCTGGCCAG AAAATGCAGG TAGCGCCACC ACCGCCACAC
    ATGCAACAGG GACAGCCATT GCCACCACAA CCGCCACAAG TGGGTGGACC CCCGCCTATA
    CAACAGCAGC AACCACCTCA GCAGCAGCAA CAACAGTCGC AGCCCCCGCC ACCGGAGCCA
    CATCAGCACC AGTTGCCAAA CGGAGGCAAA CCCCTGAGTA TGGGGCCATC TGGCGGGCAA
    CCACTAATAC CTTCATCACC CATGCAGCCA CAAGTGAGAG GGACACTGCC AGGAATGCCG
    CCAGGTAGTC AGGTTCCGCA ACCAGGAGGA GGTCCTCAGC GACAGGTACC ACCTGCGGGC
    ATGCCGATGC CCAAGCCCAA TCGCATTACA ACGGTGGCCA AACCCGTCGG TCTGGATCCC
    ATAACATTGC TGCAGGAAAG GGAGAATAGG ATAGCAGCCA GGATATCATT GCGCATGCAG
    GAGTTGCAGC GACTGCCGGC CACGATGTCC GAGGATCTGC GTCTGCAAGC GGCTATCGAG
    TTGAGGGCCC TAAGGGTCTT GAACTTCCAG CGACAGCTGC GTATGGAGTT TGTTCAGTGC
    ACGAGACGAG ATACCACTTT GGAAACAGCG TTAAACATTA AGCTCTACAA GAGGACCAAG
    AGGCAGGGCC TGCGGGAGGC ACGTGCCACC GAGAAGCTAG AGAAGCAACA GAAGCTAGAG
    GCTGAACGAA AGCGTCGCCA AAAGCATCTG GAGTTCTTGG CTGCTGTGCT TCAGCACGGC
    AAGGATCTAA GGGAGTTCCA CAGAAATAAT AAGGCCCAGC TTGCAAGGAT GAACAAGGCC
    GTGATGAATC ACCACGCCAA TGCCGAGCGT GAACAGAAGA AGGAACAAGA GCGCATCGAA
    AAGGAACGTA TGCGACGTCT GATGGCCGAG GATGAGGAGG GTTACCGCAA GCTGATTGAT
    CAAAAGAAGG ACAAGAGATT GGCTTTCCTA CTGTCGCAGA CAGATGAGTA TATTAGCAAC
    CTAACTCAGA TGGTGAAACA GCACAAGGAC GACCAGATGA AGAAAAAGGA GGAGGAGGGC
    AAGCGGCTGA TACAGTTCAA AAAGGAGCTG CTGATGAGCG GAGAGTACAT TGGCATTGAT
    GAGGGCAGCA TTGTTGCCGA TATGCGGGTA CATGTGGTGG AACAGTGCAC GGGTAAGAAA
    CTCACCGGCG ATGATGCACC CATGCTGAAG CACTTGCACC GCTGGCTGAA TATGCATCCT
    GGCTGGGATT GGATCGACGA CGAGGAGGAC AGCTGTGGGA GCAACGACGA TCATAAACCC
    AAGGTGGAGG AGCAACCAAC GGCAACAGAA GATGCCACCG ACAAAGCGCA GGCGACGGGC
    AACGATGAGG ATGCCAAGGA TCTAATTACC AAGGCCAAGG TGGAGGACGA TGAGTACAGG
    ACGGAGGAAC AGACGTACTA CAGCATCGCT CATACCATTC ATGAAAAGGT TGTGGAGCAG
    GCCAGCATCA TGGTTAATGG TACGCTCAAG GAATACCAGA TCAAAGGCTT GGAGTGGTTG
    GTTTCGCTGT ACAATAACAA TCTAAATGGT ATTTTGGCCG ATGAAATGGG TTTGGGTAAA
    ACCATTCAAA CCATTTCGCT GGTAACCTAC CTTATGGATC GAAAGAAGGT TATGGGTCCT
    TACTTGATTA TTGTACCACT CTCCACCCTG CCCAATTGGG TGCTGGAATT CGAGAAGTGG
    GCGCCAGCCG TGGGTGTAGT CAGCTACAAG GGCAGTCCGC AGGGAAGGCG ATTGCTGCAA
    AATCAAATGA GAGCAACCAA GTTCAATGTG CTGTTAACCA CATATGAGTA CGTGATTAAG
    GACAAGGCGG TGCTGGCCAA GATCCAGTGG AAATACATGA TTATCGACGA GGGTCACCGC
    ATGAAGAACC ATCACTGCAA ACTCACCCAG GTGCTGAACA CCCACTACAT TGCCCCCTAT
    CGGTTACTTC TCACGGGTAC ACCTCTGCAA AACAAGCTTC CCGAGTTGTG GGCGCTACTT
    AATTTCCTGC TGCCCTCGAT CTTTAAGTCC TGCTCCACAT TTGAACAGTG GTTCAATGCG
    CCATTTGCCA CCACTGGCGA GAAGGTCGAG CTAAACGAGG AGGAGACCAT CCTTATCATT
    CGTCGTCTGC ACAAGGTTCT TCGTCCTTTC CTGTTGCGTC GCCTCAAAAA GGAGGTAGAG
    CACCAGCTGC CCGACAAGGT CGAGTACATC ATCAAGTGCG ACATGTCCGC GCTGCAACGC
    GTTCTCTACA AGCACATGCA GAGCAAGGGT GTCCTGCTCA CCGATGGTTC CGAGAAGGGC
    AAGCACGGCA AGGGAGGCGC TAAGGCGCTG ATGAACACGA TCGTACAGCT GCGCAAGCTG
    TGCAACCATC CGTTTATGTT CCAGCACATA GAAGAGAAGT ACTGCGATCA CACTGGCGGC
    CACGGAGTTG TATCCGGACC GGATCTGTAT CGTGTCTCTG GCAAATTCGA GCTGCTTGAT
    CGCATCCTGC CCAAGCTGAA GGCGACCAAT CATCGCGTGC TGCTCTTCTG TCAGATGACC
    CAATGCATGA CCATCATCGA AGACTACCTG GGATGGCGCC AATTCGGCTA CCTTCGACTC
    GACGGTACCA CGAAGGCCGA GGATCGTGGC GAACTACTGC GAAAATTCAA CGCCAAAGGC
    TCGGATGTAT TTGTCTTTTT GCTATCTACC CGAGCTGGTG GTTTGGGTCT TAATCTGCAG
    ACCGCCGATA CTGTAGTAAT CTTCGACTCC GATTGGAATC CCCATCAGGA TCTGCAAGCC
    CAGGATCGTG CCCATCGTAT TGGTCAACGT AACGAGGTGC GCGTCCTGCG ACTAATGACA
    GTCAACTCGG TGGAAGAGCG CATCTTGGCA GCAGCCAGGT ACAAGCTGAA CATGGACGAG
    AAGGTCATCC AGGCTGGTAT GTTCGATCAG AAGTCAACGG GTAGCGAGCG ACAGCAGTTC
    CTACAAACGA TTCTGCACCA GGATGACAAC GAAGAGGAGG AGGAAAATGA GGTGCCCGAT
    GACGAAATGA TCAACATGAT GATCGCCCGC AGCGAGGAGG AGATTGAAAT ATTTAAGCGC
    ATGGATGCTG AGCGCAAGAA GGAGGATGAG GAGATCCATC CGGGTAGGGA ACGTCTGATC
    GATGAGTCCG AGCTACCCGA CTGGTTGACA AAGGATGATG ACGAAGTAGA GCGTTTCCAC
    TATCAGTACG ACGAGGATAC CATTCTAGGT CGAGGCTCCC GGCAGCGAAA GGAAGTAGAC
    TACACCGATA GTTTGACTGA AAAGGAGTGG CTGAAAGCCA TCGACGATGG CGCTGAGTTC
    GACGAGGAAG AGGAGGAAGA CGATTCGAAG CGCAAGCGAC GAAAGCGTAA GAATCGGAAG
    GAAGAGTCCG ACGATGATTC GTTGATCCTA AAACGGCGAC GACGCCAAAA TCTGGACAAG
    CGGTCCAAGA AGCAGATGCA CAAGATTATG AGTGCGGTTA TCAAGCACAA CCAAGATGGA
    CGAACCCTGT CCGAACCGTT CATGAAGTTG CCTTCGCGGC AGAGGTTGCC CGACTACTAC
    GAGATCATTA AGCGGCCAGT TGATATTAAG AAAATCCTGC AGCGCATTGA GGACTGCAAG
    TATGCCGATC TCAACGAGCT GGAGAAGGAC TTTATGCAGC TATGCCAGAA TGCACAGATC
    TACAACGAAG AGGCGTCTCT GATTTACCTG GACTCGATTG CCCTGCAAAA GGTATTCGTT
    GGGGCCAGGC AGAGGATAAC AGCTGCAGCG GATGCAGCCG CAGTGGCAGC CGGAGATAAC
    ACGGGTGAAG CGCACGGCAA TGGAGGTTCG GATAATTCGG ACAACGACGA CGACGATGGT
    GGCGATGACG GCTCCGACGA CGAGGAGATT GCAACCACTT CAGCGGCGGC TGTCAAAATG
    AAGCTGAAAC TGAACAAATC TCTGGCTAGT GCGCCCGCTA CGCCCACGCA ATCATCGTCG
    AATGTGAGCA GCGGAGCGGC TACCACATCC AAGAAGCAGA CGCGTCGCAA GCGCTCCCAA
    AAGAAGTACA CTATTTCAGA CGATGACGAC GATGACATGG ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa20962
    Clone ID Related Accession (Same CDS sequence) NM_168641.1 , NM_080498.3
    Accession Version NM_168641.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4917bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product brahma, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCCTCGC CCTCTCCGGC GAACAGTCCC ATGCCACCGC CACAGGCGCC CAGTCCGATG 
    GCCCCGCCCT CGCAGAGTCC CGCCCCCTCG CCGCACAGCC CGTATCCGCA CCAGCAGCCG
    GGTCCACTGC AAGGACCTCC GCCACCCGGA CATCCCGGTG CCTACGGACA TCCCATGCAG
    CACGGACCAC CCGGCCAGGG TCCACCCGGG CATCATATGC CACCGCACCA CCAGGGGATG
    ATTTTCTCAA AAGGTCCGCA CATGGGAATG CAGATGCCGC CGACGGGACC GAACATGTCC
    CCCTACCAAA CCCACGGAAT GCCACCCAAT GCACCCACAC AACCCTGCAT AGTGTCACCT
    GGTGGCCCAC CTGGAGGCCC GCCACCTCCG GAGCGCTCGA GCCAGGAGAA CCTGCACGCG
    CTTCAACGCG CCATTGATTC AATGGAGGAA AAGGGCTTGC AAGAGGATCC TCGCTACTCG
    CAGCTGCTGG CAATGCGAGC AACCTCGAAG CATCAGCACC TTAATGGCAA TCAGGTTAAC
    TTGTTGCGCA CACAGATCAC CGCCTATCGG TTGCTGGCGC GGAACAAGCC CATATCCATG
    CAGATGCAGC AGGCGTTGCA GGCCGCACAG CAACAGCCAC CGCCAGGACC TCCAATTGGG
    CCACCTGGAG CACCAGGCGG ACCGCCACCA GGGAGCCAGC ATGCAGGACA ACCGCCCGTG
    CCGCCGCAAC AACAGCAACA ACCGCCACCT TCAGCAGGAA CTCCACCCCA GTGCTCCACG
    CCGCCCGCAA GCAATCCTTA TGGACCGCCA GTTCCTGGCC AGAAAATGCA GGTAGCGCCA
    CCACCGCCAC ACATGCAACA GGGACAGCCA TTGCCACCAC AACCGCCACA AGTGGGTGGA
    CCCCCGCCTA TACAACAGCA GCAACCACCT CAGCAGCAGC AACAACAGTC GCAGCCCCCG
    CCACCGGAGC CACATCAGCA CCAGTTGCCA AACGGAGGCA AACCCCTGAG TATGGGGCCA
    TCTGGCGGGC AACCACTAAT ACCTTCATCA CCCATGCAGC CACAAGTGAG AGGGACACTG
    CCAGGAATGC CGCCAGGTAG TCAGGTTCCG CAACCAGGAG GAGGTCCTCA GCGACAGGTA
    CCACCTGCGG GCATGCCGAT GCCCAAGCCC AATCGCATTA CAACGGTGGC CAAACCCGTC
    GGTCTGGATC CCATAACATT GCTGCAGGAA AGGGAGAATA GGATAGCAGC CAGGATATCA
    TTGCGCATGC AGGAGTTGCA GCGACTGCCG GCCACGATGT CCGAGGATCT GCGTCTGCAA
    GCGGCTATCG AGTTGAGGGC CCTAAGGGTC TTGAACTTCC AGCGACAGCT GCGTATGGAG
    TTTGTTCAGT GCACGAGACG AGATACCACT TTGGAAACAG CGTTAAACAT TAAGCTCTAC
    AAGAGGACCA AGAGGCAGGG CCTGCGGGAG GCACGTGCCA CCGAGAAGCT AGAGAAGCAA
    CAGAAGCTAG AGGCTGAACG AAAGCGTCGC CAAAAGCATC TGGAGTTCTT GGCTGCTGTG
    CTTCAGCACG GCAAGGATCT AAGGGAGTTC CACAGAAATA ATAAGGCCCA GCTTGCAAGG
    ATGAACAAGG CCGTGATGAA TCACCACGCC AATGCCGAGC GTGAACAGAA GAAGGAACAA
    GAGCGCATCG AAAAGGAACG TATGCGACGT CTGATGGCCG AGGATGAGGA GGGTTACCGC
    AAGCTGATTG ATCAAAAGAA GGACAAGAGA TTGGCTTTCC TACTGTCGCA GACAGATGAG
    TATATTAGCA ACCTAACTCA GATGGTGAAA CAGCACAAGG ACGACCAGAT GAAGAAAAAG
    GAGGAGGAGG GCAAGCGGCT GATACAGTTC AAAAAGGAGC TGCTGATGAG CGGAGAGTAC
    ATTGGCATTG ATGAGGGCAG CATTGTTGCC GATATGCGGG TACATGTGGT GGAACAGTGC
    ACGGGTAAGA AACTCACCGG CGATGATGCA CCCATGCTGA AGCACTTGCA CCGCTGGCTG
    AATATGCATC CTGGCTGGGA TTGGATCGAC GACGAGGAGG ACAGCTGTGG GAGCAACGAC
    GATCATAAAC CCAAGGTGGA GGAGCAACCA ACGGCAACAG AAGATGCCAC CGACAAAGCG
    CAGGCGACGG GCAACGATGA GGATGCCAAG GATCTAATTA CCAAGGCCAA GGTGGAGGAC
    GATGAGTACA GGACGGAGGA ACAGACGTAC TACAGCATCG CTCATACCAT TCATGAAAAG
    GTTGTGGAGC AGGCCAGCAT CATGGTTAAT GGTACGCTCA AGGAATACCA GATCAAAGGC
    TTGGAGTGGT TGGTTTCGCT GTACAATAAC AATCTAAATG GTATTTTGGC CGATGAAATG
    GGTTTGGGTA AAACCATTCA AACCATTTCG CTGGTAACCT ACCTTATGGA TCGAAAGAAG
    GTTATGGGTC CTTACTTGAT TATTGTACCA CTCTCCACCC TGCCCAATTG GGTGCTGGAA
    TTCGAGAAGT GGGCGCCAGC CGTGGGTGTA GTCAGCTACA AGGGCAGTCC GCAGGGAAGG
    CGATTGCTGC AAAATCAAAT GAGAGCAACC AAGTTCAATG TGCTGTTAAC CACATATGAG
    TACGTGATTA AGGACAAGGC GGTGCTGGCC AAGATCCAGT GGAAATACAT GATTATCGAC
    GAGGGTCACC GCATGAAGAA CCATCACTGC AAACTCACCC AGGTGCTGAA CACCCACTAC
    ATTGCCCCCT ATCGGTTACT TCTCACGGGT ACACCTCTGC AAAACAAGCT TCCCGAGTTG
    TGGGCGCTAC TTAATTTCCT GCTGCCCTCG ATCTTTAAGT CCTGCTCCAC ATTTGAACAG
    TGGTTCAATG CGCCATTTGC CACCACTGGC GAGAAGGTCG AGCTAAACGA GGAGGAGACC
    ATCCTTATCA TTCGTCGTCT GCACAAGGTT CTTCGTCCTT TCCTGTTGCG TCGCCTCAAA
    AAGGAGGTAG AGCACCAGCT GCCCGACAAG GTCGAGTACA TCATCAAGTG CGACATGTCC
    GCGCTGCAAC GCGTTCTCTA CAAGCACATG CAGAGCAAGG GTGTCCTGCT CACCGATGGT
    TCCGAGAAGG GCAAGCACGG CAAGGGAGGC GCTAAGGCGC TGATGAACAC GATCGTACAG
    CTGCGCAAGC TGTGCAACCA TCCGTTTATG TTCCAGCACA TAGAAGAGAA GTACTGCGAT
    CACACTGGCG GCCACGGAGT TGTATCCGGA CCGGATCTGT ATCGTGTCTC TGGCAAATTC
    GAGCTGCTTG ATCGCATCCT GCCCAAGCTG AAGGCGACCA ATCATCGCGT GCTGCTCTTC
    TGTCAGATGA CCCAATGCAT GACCATCATC GAAGACTACC TGGGATGGCG CCAATTCGGC
    TACCTTCGAC TCGACGGTAC CACGAAGGCC GAGGATCGTG GCGAACTACT GCGAAAATTC
    AACGCCAAAG GCTCGGATGT ATTTGTCTTT TTGCTATCTA CCCGAGCTGG TGGTTTGGGT
    CTTAATCTGC AGACCGCCGA TACTGTAGTA ATCTTCGACT CCGATTGGAA TCCCCATCAG
    GATCTGCAAG CCCAGGATCG TGCCCATCGT ATTGGTCAAC GTAACGAGGT GCGCGTCCTG
    CGACTAATGA CAGTCAACTC GGTGGAAGAG CGCATCTTGG CAGCAGCCAG GTACAAGCTG
    AACATGGACG AGAAGGTCAT CCAGGCTGGT ATGTTCGATC AGAAGTCAAC GGGTAGCGAG
    CGACAGCAGT TCCTACAAAC GATTCTGCAC CAGGATGACA ACGAAGAGGA GGAGGAAAAT
    GAGGTGCCCG ATGACGAAAT GATCAACATG ATGATCGCCC GCAGCGAGGA GGAGATTGAA
    ATATTTAAGC GCATGGATGC TGAGCGCAAG AAGGAGGATG AGGAGATCCA TCCGGGTAGG
    GAACGTCTGA TCGATGAGTC CGAGCTACCC GACTGGTTGA CAAAGGATGA TGACGAAGTA
    GAGCGTTTCC ACTATCAGTA CGACGAGGAT ACCATTCTAG GTCGAGGCTC CCGGCAGCGA
    AAGGAAGTAG ACTACACCGA TAGTTTGACT GAAAAGGAGT GGCTGAAAGC CATCGACGAT
    GGCGCTGAGT TCGACGAGGA AGAGGAGGAA GACGATTCGA AGCGCAAGCG ACGAAAGCGT
    AAGAATCGGA AGGAAGAGTC CGACGATGAT TCGTTGATCC TAAAACGGCG ACGACGCCAA
    AATCTGGACA AGCGGTCCAA GAAGCAGATG CACAAGATTA TGAGTGCGGT TATCAAGCAC
    AACCAAGATG GACGAACCCT GTCCGAACCG TTCATGAAGT TGCCTTCGCG GCAGAGGTTG
    CCCGACTACT ACGAGATCAT TAAGCGGCCA GTTGATATTA AGAAAATCCT GCAGCGCATT
    GAGGACTGCA AGTATGCCGA TCTCAACGAG CTGGAGAAGG ACTTTATGCA GCTATGCCAG
    AATGCACAGA TCTACAACGA AGAGGCGTCT CTGATTTACC TGGACTCGAT TGCCCTGCAA
    AAGGTATTCG TTGGGGCCAG GCAGAGGATA ACAGCTGCAG CGGATGCAGC CGCAGTGGCA
    GCCGGAGATA ACACGGGTGA AGCGCACGGC AATGGAGGTT CGGATAATTC GGACAACGAC
    GACGACGATG GTGGCGATGA CGGCTCCGAC GACGAGGAGA TTGCAACCAC TTCAGCGGCG
    GCTGTCAAAA TGAAGCTGAA ACTGAACAAA TCTCTGGCTA GTGCGCCCGC TACGCCCACG
    CAATCATCGT CGAATGTGAG CAGCGGAGCG GCTACCACAT CCAAGAAGCA GACGCGTCGC
    AAGCGCTCCC AAAAGAAGTA CACTATTTCA GACGATGACG ACGATGACAT GGACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_730089.1
    CDS342..5258
    Translation

    Target ORF information:

    RefSeq Version NM_168641.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster brahma (brm), transcript variant B, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_168641.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCCTCGC CCTCTCCGGC GAACAGTCCC ATGCCACCGC CACAGGCGCC CAGTCCGATG 
    GCCCCGCCCT CGCAGAGTCC CGCCCCCTCG CCGCACAGCC CGTATCCGCA CCAGCAGCCG
    GGTCCACTGC AAGGACCTCC GCCACCCGGA CATCCCGGTG CCTACGGACA TCCCATGCAG
    CACGGACCAC CCGGCCAGGG TCCACCCGGG CATCATATGC CACCGCACCA CCAGGGGATG
    ATTTTCTCAA AAGGTCCGCA CATGGGAATG CAGATGCCGC CGACGGGACC GAACATGTCC
    CCCTACCAAA CCCACGGAAT GCCACCCAAT GCACCCACAC AACCCTGCAT AGTGTCACCT
    GGTGGCCCAC CTGGAGGCCC GCCACCTCCG GAGCGCTCGA GCCAGGAGAA CCTGCACGCG
    CTTCAACGCG CCATTGATTC AATGGAGGAA AAGGGCTTGC AAGAGGATCC TCGCTACTCG
    CAGCTGCTGG CAATGCGAGC AACCTCGAAG CATCAGCACC TTAATGGCAA TCAGGTTAAC
    TTGTTGCGCA CACAGATCAC CGCCTATCGG TTGCTGGCGC GGAACAAGCC CATATCCATG
    CAGATGCAGC AGGCGTTGCA GGCCGCACAG CAACAGCCAC CGCCAGGACC TCCAATTGGG
    CCACCTGGAG CACCAGGCGG ACCGCCACCA GGGAGCCAGC ATGCAGGACA ACCGCCCGTG
    CCGCCGCAAC AACAGCAACA ACCGCCACCT TCAGCAGGAA CTCCACCCCA GTGCTCCACG
    CCGCCCGCAA GCAATCCTTA TGGACCGCCA GTTCCTGGCC AGAAAATGCA GGTAGCGCCA
    CCACCGCCAC ACATGCAACA GGGACAGCCA TTGCCACCAC AACCGCCACA AGTGGGTGGA
    CCCCCGCCTA TACAACAGCA GCAACCACCT CAGCAGCAGC AACAACAGTC GCAGCCCCCG
    CCACCGGAGC CACATCAGCA CCAGTTGCCA AACGGAGGCA AACCCCTGAG TATGGGGCCA
    TCTGGCGGGC AACCACTAAT ACCTTCATCA CCCATGCAGC CACAAGTGAG AGGGACACTG
    CCAGGAATGC CGCCAGGTAG TCAGGTTCCG CAACCAGGAG GAGGTCCTCA GCGACAGGTA
    CCACCTGCGG GCATGCCGAT GCCCAAGCCC AATCGCATTA CAACGGTGGC CAAACCCGTC
    GGTCTGGATC CCATAACATT GCTGCAGGAA AGGGAGAATA GGATAGCAGC CAGGATATCA
    TTGCGCATGC AGGAGTTGCA GCGACTGCCG GCCACGATGT CCGAGGATCT GCGTCTGCAA
    GCGGCTATCG AGTTGAGGGC CCTAAGGGTC TTGAACTTCC AGCGACAGCT GCGTATGGAG
    TTTGTTCAGT GCACGAGACG AGATACCACT TTGGAAACAG CGTTAAACAT TAAGCTCTAC
    AAGAGGACCA AGAGGCAGGG CCTGCGGGAG GCACGTGCCA CCGAGAAGCT AGAGAAGCAA
    CAGAAGCTAG AGGCTGAACG AAAGCGTCGC CAAAAGCATC TGGAGTTCTT GGCTGCTGTG
    CTTCAGCACG GCAAGGATCT AAGGGAGTTC CACAGAAATA ATAAGGCCCA GCTTGCAAGG
    ATGAACAAGG CCGTGATGAA TCACCACGCC AATGCCGAGC GTGAACAGAA GAAGGAACAA
    GAGCGCATCG AAAAGGAACG TATGCGACGT CTGATGGCCG AGGATGAGGA GGGTTACCGC
    AAGCTGATTG ATCAAAAGAA GGACAAGAGA TTGGCTTTCC TACTGTCGCA GACAGATGAG
    TATATTAGCA ACCTAACTCA GATGGTGAAA CAGCACAAGG ACGACCAGAT GAAGAAAAAG
    GAGGAGGAGG GCAAGCGGCT GATACAGTTC AAAAAGGAGC TGCTGATGAG CGGAGAGTAC
    ATTGGCATTG ATGAGGGCAG CATTGTTGCC GATATGCGGG TACATGTGGT GGAACAGTGC
    ACGGGTAAGA AACTCACCGG CGATGATGCA CCCATGCTGA AGCACTTGCA CCGCTGGCTG
    AATATGCATC CTGGCTGGGA TTGGATCGAC GACGAGGAGG ACAGCTGTGG GAGCAACGAC
    GATCATAAAC CCAAGGTGGA GGAGCAACCA ACGGCAACAG AAGATGCCAC CGACAAAGCG
    CAGGCGACGG GCAACGATGA GGATGCCAAG GATCTAATTA CCAAGGCCAA GGTGGAGGAC
    GATGAGTACA GGACGGAGGA ACAGACGTAC TACAGCATCG CTCATACCAT TCATGAAAAG
    GTTGTGGAGC AGGCCAGCAT CATGGTTAAT GGTACGCTCA AGGAATACCA GATCAAAGGC
    TTGGAGTGGT TGGTTTCGCT GTACAATAAC AATCTAAATG GTATTTTGGC CGATGAAATG
    GGTTTGGGTA AAACCATTCA AACCATTTCG CTGGTAACCT ACCTTATGGA TCGAAAGAAG
    GTTATGGGTC CTTACTTGAT TATTGTACCA CTCTCCACCC TGCCCAATTG GGTGCTGGAA
    TTCGAGAAGT GGGCGCCAGC CGTGGGTGTA GTCAGCTACA AGGGCAGTCC GCAGGGAAGG
    CGATTGCTGC AAAATCAAAT GAGAGCAACC AAGTTCAATG TGCTGTTAAC CACATATGAG
    TACGTGATTA AGGACAAGGC GGTGCTGGCC AAGATCCAGT GGAAATACAT GATTATCGAC
    GAGGGTCACC GCATGAAGAA CCATCACTGC AAACTCACCC AGGTGCTGAA CACCCACTAC
    ATTGCCCCCT ATCGGTTACT TCTCACGGGT ACACCTCTGC AAAACAAGCT TCCCGAGTTG
    TGGGCGCTAC TTAATTTCCT GCTGCCCTCG ATCTTTAAGT CCTGCTCCAC ATTTGAACAG
    TGGTTCAATG CGCCATTTGC CACCACTGGC GAGAAGGTCG AGCTAAACGA GGAGGAGACC
    ATCCTTATCA TTCGTCGTCT GCACAAGGTT CTTCGTCCTT TCCTGTTGCG TCGCCTCAAA
    AAGGAGGTAG AGCACCAGCT GCCCGACAAG GTCGAGTACA TCATCAAGTG CGACATGTCC
    GCGCTGCAAC GCGTTCTCTA CAAGCACATG CAGAGCAAGG GTGTCCTGCT CACCGATGGT
    TCCGAGAAGG GCAAGCACGG CAAGGGAGGC GCTAAGGCGC TGATGAACAC GATCGTACAG
    CTGCGCAAGC TGTGCAACCA TCCGTTTATG TTCCAGCACA TAGAAGAGAA GTACTGCGAT
    CACACTGGCG GCCACGGAGT TGTATCCGGA CCGGATCTGT ATCGTGTCTC TGGCAAATTC
    GAGCTGCTTG ATCGCATCCT GCCCAAGCTG AAGGCGACCA ATCATCGCGT GCTGCTCTTC
    TGTCAGATGA CCCAATGCAT GACCATCATC GAAGACTACC TGGGATGGCG CCAATTCGGC
    TACCTTCGAC TCGACGGTAC CACGAAGGCC GAGGATCGTG GCGAACTACT GCGAAAATTC
    AACGCCAAAG GCTCGGATGT ATTTGTCTTT TTGCTATCTA CCCGAGCTGG TGGTTTGGGT
    CTTAATCTGC AGACCGCCGA TACTGTAGTA ATCTTCGACT CCGATTGGAA TCCCCATCAG
    GATCTGCAAG CCCAGGATCG TGCCCATCGT ATTGGTCAAC GTAACGAGGT GCGCGTCCTG
    CGACTAATGA CAGTCAACTC GGTGGAAGAG CGCATCTTGG CAGCAGCCAG GTACAAGCTG
    AACATGGACG AGAAGGTCAT CCAGGCTGGT ATGTTCGATC AGAAGTCAAC GGGTAGCGAG
    CGACAGCAGT TCCTACAAAC GATTCTGCAC CAGGATGACA ACGAAGAGGA GGAGGAAAAT
    GAGGTGCCCG ATGACGAAAT GATCAACATG ATGATCGCCC GCAGCGAGGA GGAGATTGAA
    ATATTTAAGC GCATGGATGC TGAGCGCAAG AAGGAGGATG AGGAGATCCA TCCGGGTAGG
    GAACGTCTGA TCGATGAGTC CGAGCTACCC GACTGGTTGA CAAAGGATGA TGACGAAGTA
    GAGCGTTTCC ACTATCAGTA CGACGAGGAT ACCATTCTAG GTCGAGGCTC CCGGCAGCGA
    AAGGAAGTAG ACTACACCGA TAGTTTGACT GAAAAGGAGT GGCTGAAAGC CATCGACGAT
    GGCGCTGAGT TCGACGAGGA AGAGGAGGAA GACGATTCGA AGCGCAAGCG ACGAAAGCGT
    AAGAATCGGA AGGAAGAGTC CGACGATGAT TCGTTGATCC TAAAACGGCG ACGACGCCAA
    AATCTGGACA AGCGGTCCAA GAAGCAGATG CACAAGATTA TGAGTGCGGT TATCAAGCAC
    AACCAAGATG GACGAACCCT GTCCGAACCG TTCATGAAGT TGCCTTCGCG GCAGAGGTTG
    CCCGACTACT ACGAGATCAT TAAGCGGCCA GTTGATATTA AGAAAATCCT GCAGCGCATT
    GAGGACTGCA AGTATGCCGA TCTCAACGAG CTGGAGAAGG ACTTTATGCA GCTATGCCAG
    AATGCACAGA TCTACAACGA AGAGGCGTCT CTGATTTACC TGGACTCGAT TGCCCTGCAA
    AAGGTATTCG TTGGGGCCAG GCAGAGGATA ACAGCTGCAG CGGATGCAGC CGCAGTGGCA
    GCCGGAGATA ACACGGGTGA AGCGCACGGC AATGGAGGTT CGGATAATTC GGACAACGAC
    GACGACGATG GTGGCGATGA CGGCTCCGAC GACGAGGAGA TTGCAACCAC TTCAGCGGCG
    GCTGTCAAAA TGAAGCTGAA ACTGAACAAA TCTCTGGCTA GTGCGCCCGC TACGCCCACG
    CAATCATCGT CGAATGTGAG CAGCGGAGCG GCTACCACAT CCAAGAAGCA GACGCGTCGC
    AAGCGCTCCC AAAAGAAGTA CACTATTTCA GACGATGACG ACGATGACAT GGACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa20962
    Clone ID Related Accession (Same CDS sequence) NM_168641.1 , NM_080498.3
    Accession Version NM_080498.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4917bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product brahma, isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_080498.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCCTCGC CCTCTCCGGC GAACAGTCCC ATGCCACCGC CACAGGCGCC CAGTCCGATG 
    GCCCCGCCCT CGCAGAGTCC CGCCCCCTCG CCGCACAGCC CGTATCCGCA CCAGCAGCCG
    GGTCCACTGC AAGGACCTCC GCCACCCGGA CATCCCGGTG CCTACGGACA TCCCATGCAG
    CACGGACCAC CCGGCCAGGG TCCACCCGGG CATCATATGC CACCGCACCA CCAGGGGATG
    ATTTTCTCAA AAGGTCCGCA CATGGGAATG CAGATGCCGC CGACGGGACC GAACATGTCC
    CCCTACCAAA CCCACGGAAT GCCACCCAAT GCACCCACAC AACCCTGCAT AGTGTCACCT
    GGTGGCCCAC CTGGAGGCCC GCCACCTCCG GAGCGCTCGA GCCAGGAGAA CCTGCACGCG
    CTTCAACGCG CCATTGATTC AATGGAGGAA AAGGGCTTGC AAGAGGATCC TCGCTACTCG
    CAGCTGCTGG CAATGCGAGC AACCTCGAAG CATCAGCACC TTAATGGCAA TCAGGTTAAC
    TTGTTGCGCA CACAGATCAC CGCCTATCGG TTGCTGGCGC GGAACAAGCC CATATCCATG
    CAGATGCAGC AGGCGTTGCA GGCCGCACAG CAACAGCCAC CGCCAGGACC TCCAATTGGG
    CCACCTGGAG CACCAGGCGG ACCGCCACCA GGGAGCCAGC ATGCAGGACA ACCGCCCGTG
    CCGCCGCAAC AACAGCAACA ACCGCCACCT TCAGCAGGAA CTCCACCCCA GTGCTCCACG
    CCGCCCGCAA GCAATCCTTA TGGACCGCCA GTTCCTGGCC AGAAAATGCA GGTAGCGCCA
    CCACCGCCAC ACATGCAACA GGGACAGCCA TTGCCACCAC AACCGCCACA AGTGGGTGGA
    CCCCCGCCTA TACAACAGCA GCAACCACCT CAGCAGCAGC AACAACAGTC GCAGCCCCCG
    CCACCGGAGC CACATCAGCA CCAGTTGCCA AACGGAGGCA AACCCCTGAG TATGGGGCCA
    TCTGGCGGGC AACCACTAAT ACCTTCATCA CCCATGCAGC CACAAGTGAG AGGGACACTG
    CCAGGAATGC CGCCAGGTAG TCAGGTTCCG CAACCAGGAG GAGGTCCTCA GCGACAGGTA
    CCACCTGCGG GCATGCCGAT GCCCAAGCCC AATCGCATTA CAACGGTGGC CAAACCCGTC
    GGTCTGGATC CCATAACATT GCTGCAGGAA AGGGAGAATA GGATAGCAGC CAGGATATCA
    TTGCGCATGC AGGAGTTGCA GCGACTGCCG GCCACGATGT CCGAGGATCT GCGTCTGCAA
    GCGGCTATCG AGTTGAGGGC CCTAAGGGTC TTGAACTTCC AGCGACAGCT GCGTATGGAG
    TTTGTTCAGT GCACGAGACG AGATACCACT TTGGAAACAG CGTTAAACAT TAAGCTCTAC
    AAGAGGACCA AGAGGCAGGG CCTGCGGGAG GCACGTGCCA CCGAGAAGCT AGAGAAGCAA
    CAGAAGCTAG AGGCTGAACG AAAGCGTCGC CAAAAGCATC TGGAGTTCTT GGCTGCTGTG
    CTTCAGCACG GCAAGGATCT AAGGGAGTTC CACAGAAATA ATAAGGCCCA GCTTGCAAGG
    ATGAACAAGG CCGTGATGAA TCACCACGCC AATGCCGAGC GTGAACAGAA GAAGGAACAA
    GAGCGCATCG AAAAGGAACG TATGCGACGT CTGATGGCCG AGGATGAGGA GGGTTACCGC
    AAGCTGATTG ATCAAAAGAA GGACAAGAGA TTGGCTTTCC TACTGTCGCA GACAGATGAG
    TATATTAGCA ACCTAACTCA GATGGTGAAA CAGCACAAGG ACGACCAGAT GAAGAAAAAG
    GAGGAGGAGG GCAAGCGGCT GATACAGTTC AAAAAGGAGC TGCTGATGAG CGGAGAGTAC
    ATTGGCATTG ATGAGGGCAG CATTGTTGCC GATATGCGGG TACATGTGGT GGAACAGTGC
    ACGGGTAAGA AACTCACCGG CGATGATGCA CCCATGCTGA AGCACTTGCA CCGCTGGCTG
    AATATGCATC CTGGCTGGGA TTGGATCGAC GACGAGGAGG ACAGCTGTGG GAGCAACGAC
    GATCATAAAC CCAAGGTGGA GGAGCAACCA ACGGCAACAG AAGATGCCAC CGACAAAGCG
    CAGGCGACGG GCAACGATGA GGATGCCAAG GATCTAATTA CCAAGGCCAA GGTGGAGGAC
    GATGAGTACA GGACGGAGGA ACAGACGTAC TACAGCATCG CTCATACCAT TCATGAAAAG
    GTTGTGGAGC AGGCCAGCAT CATGGTTAAT GGTACGCTCA AGGAATACCA GATCAAAGGC
    TTGGAGTGGT TGGTTTCGCT GTACAATAAC AATCTAAATG GTATTTTGGC CGATGAAATG
    GGTTTGGGTA AAACCATTCA AACCATTTCG CTGGTAACCT ACCTTATGGA TCGAAAGAAG
    GTTATGGGTC CTTACTTGAT TATTGTACCA CTCTCCACCC TGCCCAATTG GGTGCTGGAA
    TTCGAGAAGT GGGCGCCAGC CGTGGGTGTA GTCAGCTACA AGGGCAGTCC GCAGGGAAGG
    CGATTGCTGC AAAATCAAAT GAGAGCAACC AAGTTCAATG TGCTGTTAAC CACATATGAG
    TACGTGATTA AGGACAAGGC GGTGCTGGCC AAGATCCAGT GGAAATACAT GATTATCGAC
    GAGGGTCACC GCATGAAGAA CCATCACTGC AAACTCACCC AGGTGCTGAA CACCCACTAC
    ATTGCCCCCT ATCGGTTACT TCTCACGGGT ACACCTCTGC AAAACAAGCT TCCCGAGTTG
    TGGGCGCTAC TTAATTTCCT GCTGCCCTCG ATCTTTAAGT CCTGCTCCAC ATTTGAACAG
    TGGTTCAATG CGCCATTTGC CACCACTGGC GAGAAGGTCG AGCTAAACGA GGAGGAGACC
    ATCCTTATCA TTCGTCGTCT GCACAAGGTT CTTCGTCCTT TCCTGTTGCG TCGCCTCAAA
    AAGGAGGTAG AGCACCAGCT GCCCGACAAG GTCGAGTACA TCATCAAGTG CGACATGTCC
    GCGCTGCAAC GCGTTCTCTA CAAGCACATG CAGAGCAAGG GTGTCCTGCT CACCGATGGT
    TCCGAGAAGG GCAAGCACGG CAAGGGAGGC GCTAAGGCGC TGATGAACAC GATCGTACAG
    CTGCGCAAGC TGTGCAACCA TCCGTTTATG TTCCAGCACA TAGAAGAGAA GTACTGCGAT
    CACACTGGCG GCCACGGAGT TGTATCCGGA CCGGATCTGT ATCGTGTCTC TGGCAAATTC
    GAGCTGCTTG ATCGCATCCT GCCCAAGCTG AAGGCGACCA ATCATCGCGT GCTGCTCTTC
    TGTCAGATGA CCCAATGCAT GACCATCATC GAAGACTACC TGGGATGGCG CCAATTCGGC
    TACCTTCGAC TCGACGGTAC CACGAAGGCC GAGGATCGTG GCGAACTACT GCGAAAATTC
    AACGCCAAAG GCTCGGATGT ATTTGTCTTT TTGCTATCTA CCCGAGCTGG TGGTTTGGGT
    CTTAATCTGC AGACCGCCGA TACTGTAGTA ATCTTCGACT CCGATTGGAA TCCCCATCAG
    GATCTGCAAG CCCAGGATCG TGCCCATCGT ATTGGTCAAC GTAACGAGGT GCGCGTCCTG
    CGACTAATGA CAGTCAACTC GGTGGAAGAG CGCATCTTGG CAGCAGCCAG GTACAAGCTG
    AACATGGACG AGAAGGTCAT CCAGGCTGGT ATGTTCGATC AGAAGTCAAC GGGTAGCGAG
    CGACAGCAGT TCCTACAAAC GATTCTGCAC CAGGATGACA ACGAAGAGGA GGAGGAAAAT
    GAGGTGCCCG ATGACGAAAT GATCAACATG ATGATCGCCC GCAGCGAGGA GGAGATTGAA
    ATATTTAAGC GCATGGATGC TGAGCGCAAG AAGGAGGATG AGGAGATCCA TCCGGGTAGG
    GAACGTCTGA TCGATGAGTC CGAGCTACCC GACTGGTTGA CAAAGGATGA TGACGAAGTA
    GAGCGTTTCC ACTATCAGTA CGACGAGGAT ACCATTCTAG GTCGAGGCTC CCGGCAGCGA
    AAGGAAGTAG ACTACACCGA TAGTTTGACT GAAAAGGAGT GGCTGAAAGC CATCGACGAT
    GGCGCTGAGT TCGACGAGGA AGAGGAGGAA GACGATTCGA AGCGCAAGCG ACGAAAGCGT
    AAGAATCGGA AGGAAGAGTC CGACGATGAT TCGTTGATCC TAAAACGGCG ACGACGCCAA
    AATCTGGACA AGCGGTCCAA GAAGCAGATG CACAAGATTA TGAGTGCGGT TATCAAGCAC
    AACCAAGATG GACGAACCCT GTCCGAACCG TTCATGAAGT TGCCTTCGCG GCAGAGGTTG
    CCCGACTACT ACGAGATCAT TAAGCGGCCA GTTGATATTA AGAAAATCCT GCAGCGCATT
    GAGGACTGCA AGTATGCCGA TCTCAACGAG CTGGAGAAGG ACTTTATGCA GCTATGCCAG
    AATGCACAGA TCTACAACGA AGAGGCGTCT CTGATTTACC TGGACTCGAT TGCCCTGCAA
    AAGGTATTCG TTGGGGCCAG GCAGAGGATA ACAGCTGCAG CGGATGCAGC CGCAGTGGCA
    GCCGGAGATA ACACGGGTGA AGCGCACGGC AATGGAGGTT CGGATAATTC GGACAACGAC
    GACGACGATG GTGGCGATGA CGGCTCCGAC GACGAGGAGA TTGCAACCAC TTCAGCGGCG
    GCTGTCAAAA TGAAGCTGAA ACTGAACAAA TCTCTGGCTA GTGCGCCCGC TACGCCCACG
    CAATCATCGT CGAATGTGAG CAGCGGAGCG GCTACCACAT CCAAGAAGCA GACGCGTCGC
    AAGCGCTCCC AAAAGAAGTA CACTATTTCA GACGATGACG ACGATGACAT GGACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_536746.1
    CDS128..5044
    Translation

    Target ORF information:

    RefSeq Version NM_080498.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster brahma (brm), transcript variant A, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_080498.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCCTCGC CCTCTCCGGC GAACAGTCCC ATGCCACCGC CACAGGCGCC CAGTCCGATG 
    GCCCCGCCCT CGCAGAGTCC CGCCCCCTCG CCGCACAGCC CGTATCCGCA CCAGCAGCCG
    GGTCCACTGC AAGGACCTCC GCCACCCGGA CATCCCGGTG CCTACGGACA TCCCATGCAG
    CACGGACCAC CCGGCCAGGG TCCACCCGGG CATCATATGC CACCGCACCA CCAGGGGATG
    ATTTTCTCAA AAGGTCCGCA CATGGGAATG CAGATGCCGC CGACGGGACC GAACATGTCC
    CCCTACCAAA CCCACGGAAT GCCACCCAAT GCACCCACAC AACCCTGCAT AGTGTCACCT
    GGTGGCCCAC CTGGAGGCCC GCCACCTCCG GAGCGCTCGA GCCAGGAGAA CCTGCACGCG
    CTTCAACGCG CCATTGATTC AATGGAGGAA AAGGGCTTGC AAGAGGATCC TCGCTACTCG
    CAGCTGCTGG CAATGCGAGC AACCTCGAAG CATCAGCACC TTAATGGCAA TCAGGTTAAC
    TTGTTGCGCA CACAGATCAC CGCCTATCGG TTGCTGGCGC GGAACAAGCC CATATCCATG
    CAGATGCAGC AGGCGTTGCA GGCCGCACAG CAACAGCCAC CGCCAGGACC TCCAATTGGG
    CCACCTGGAG CACCAGGCGG ACCGCCACCA GGGAGCCAGC ATGCAGGACA ACCGCCCGTG
    CCGCCGCAAC AACAGCAACA ACCGCCACCT TCAGCAGGAA CTCCACCCCA GTGCTCCACG
    CCGCCCGCAA GCAATCCTTA TGGACCGCCA GTTCCTGGCC AGAAAATGCA GGTAGCGCCA
    CCACCGCCAC ACATGCAACA GGGACAGCCA TTGCCACCAC AACCGCCACA AGTGGGTGGA
    CCCCCGCCTA TACAACAGCA GCAACCACCT CAGCAGCAGC AACAACAGTC GCAGCCCCCG
    CCACCGGAGC CACATCAGCA CCAGTTGCCA AACGGAGGCA AACCCCTGAG TATGGGGCCA
    TCTGGCGGGC AACCACTAAT ACCTTCATCA CCCATGCAGC CACAAGTGAG AGGGACACTG
    CCAGGAATGC CGCCAGGTAG TCAGGTTCCG CAACCAGGAG GAGGTCCTCA GCGACAGGTA
    CCACCTGCGG GCATGCCGAT GCCCAAGCCC AATCGCATTA CAACGGTGGC CAAACCCGTC
    GGTCTGGATC CCATAACATT GCTGCAGGAA AGGGAGAATA GGATAGCAGC CAGGATATCA
    TTGCGCATGC AGGAGTTGCA GCGACTGCCG GCCACGATGT CCGAGGATCT GCGTCTGCAA
    GCGGCTATCG AGTTGAGGGC CCTAAGGGTC TTGAACTTCC AGCGACAGCT GCGTATGGAG
    TTTGTTCAGT GCACGAGACG AGATACCACT TTGGAAACAG CGTTAAACAT TAAGCTCTAC
    AAGAGGACCA AGAGGCAGGG CCTGCGGGAG GCACGTGCCA CCGAGAAGCT AGAGAAGCAA
    CAGAAGCTAG AGGCTGAACG AAAGCGTCGC CAAAAGCATC TGGAGTTCTT GGCTGCTGTG
    CTTCAGCACG GCAAGGATCT AAGGGAGTTC CACAGAAATA ATAAGGCCCA GCTTGCAAGG
    ATGAACAAGG CCGTGATGAA TCACCACGCC AATGCCGAGC GTGAACAGAA GAAGGAACAA
    GAGCGCATCG AAAAGGAACG TATGCGACGT CTGATGGCCG AGGATGAGGA GGGTTACCGC
    AAGCTGATTG ATCAAAAGAA GGACAAGAGA TTGGCTTTCC TACTGTCGCA GACAGATGAG
    TATATTAGCA ACCTAACTCA GATGGTGAAA CAGCACAAGG ACGACCAGAT GAAGAAAAAG
    GAGGAGGAGG GCAAGCGGCT GATACAGTTC AAAAAGGAGC TGCTGATGAG CGGAGAGTAC
    ATTGGCATTG ATGAGGGCAG CATTGTTGCC GATATGCGGG TACATGTGGT GGAACAGTGC
    ACGGGTAAGA AACTCACCGG CGATGATGCA CCCATGCTGA AGCACTTGCA CCGCTGGCTG
    AATATGCATC CTGGCTGGGA TTGGATCGAC GACGAGGAGG ACAGCTGTGG GAGCAACGAC
    GATCATAAAC CCAAGGTGGA GGAGCAACCA ACGGCAACAG AAGATGCCAC CGACAAAGCG
    CAGGCGACGG GCAACGATGA GGATGCCAAG GATCTAATTA CCAAGGCCAA GGTGGAGGAC
    GATGAGTACA GGACGGAGGA ACAGACGTAC TACAGCATCG CTCATACCAT TCATGAAAAG
    GTTGTGGAGC AGGCCAGCAT CATGGTTAAT GGTACGCTCA AGGAATACCA GATCAAAGGC
    TTGGAGTGGT TGGTTTCGCT GTACAATAAC AATCTAAATG GTATTTTGGC CGATGAAATG
    GGTTTGGGTA AAACCATTCA AACCATTTCG CTGGTAACCT ACCTTATGGA TCGAAAGAAG
    GTTATGGGTC CTTACTTGAT TATTGTACCA CTCTCCACCC TGCCCAATTG GGTGCTGGAA
    TTCGAGAAGT GGGCGCCAGC CGTGGGTGTA GTCAGCTACA AGGGCAGTCC GCAGGGAAGG
    CGATTGCTGC AAAATCAAAT GAGAGCAACC AAGTTCAATG TGCTGTTAAC CACATATGAG
    TACGTGATTA AGGACAAGGC GGTGCTGGCC AAGATCCAGT GGAAATACAT GATTATCGAC
    GAGGGTCACC GCATGAAGAA CCATCACTGC AAACTCACCC AGGTGCTGAA CACCCACTAC
    ATTGCCCCCT ATCGGTTACT TCTCACGGGT ACACCTCTGC AAAACAAGCT TCCCGAGTTG
    TGGGCGCTAC TTAATTTCCT GCTGCCCTCG ATCTTTAAGT CCTGCTCCAC ATTTGAACAG
    TGGTTCAATG CGCCATTTGC CACCACTGGC GAGAAGGTCG AGCTAAACGA GGAGGAGACC
    ATCCTTATCA TTCGTCGTCT GCACAAGGTT CTTCGTCCTT TCCTGTTGCG TCGCCTCAAA
    AAGGAGGTAG AGCACCAGCT GCCCGACAAG GTCGAGTACA TCATCAAGTG CGACATGTCC
    GCGCTGCAAC GCGTTCTCTA CAAGCACATG CAGAGCAAGG GTGTCCTGCT CACCGATGGT
    TCCGAGAAGG GCAAGCACGG CAAGGGAGGC GCTAAGGCGC TGATGAACAC GATCGTACAG
    CTGCGCAAGC TGTGCAACCA TCCGTTTATG TTCCAGCACA TAGAAGAGAA GTACTGCGAT
    CACACTGGCG GCCACGGAGT TGTATCCGGA CCGGATCTGT ATCGTGTCTC TGGCAAATTC
    GAGCTGCTTG ATCGCATCCT GCCCAAGCTG AAGGCGACCA ATCATCGCGT GCTGCTCTTC
    TGTCAGATGA CCCAATGCAT GACCATCATC GAAGACTACC TGGGATGGCG CCAATTCGGC
    TACCTTCGAC TCGACGGTAC CACGAAGGCC GAGGATCGTG GCGAACTACT GCGAAAATTC
    AACGCCAAAG GCTCGGATGT ATTTGTCTTT TTGCTATCTA CCCGAGCTGG TGGTTTGGGT
    CTTAATCTGC AGACCGCCGA TACTGTAGTA ATCTTCGACT CCGATTGGAA TCCCCATCAG
    GATCTGCAAG CCCAGGATCG TGCCCATCGT ATTGGTCAAC GTAACGAGGT GCGCGTCCTG
    CGACTAATGA CAGTCAACTC GGTGGAAGAG CGCATCTTGG CAGCAGCCAG GTACAAGCTG
    AACATGGACG AGAAGGTCAT CCAGGCTGGT ATGTTCGATC AGAAGTCAAC GGGTAGCGAG
    CGACAGCAGT TCCTACAAAC GATTCTGCAC CAGGATGACA ACGAAGAGGA GGAGGAAAAT
    GAGGTGCCCG ATGACGAAAT GATCAACATG ATGATCGCCC GCAGCGAGGA GGAGATTGAA
    ATATTTAAGC GCATGGATGC TGAGCGCAAG AAGGAGGATG AGGAGATCCA TCCGGGTAGG
    GAACGTCTGA TCGATGAGTC CGAGCTACCC GACTGGTTGA CAAAGGATGA TGACGAAGTA
    GAGCGTTTCC ACTATCAGTA CGACGAGGAT ACCATTCTAG GTCGAGGCTC CCGGCAGCGA
    AAGGAAGTAG ACTACACCGA TAGTTTGACT GAAAAGGAGT GGCTGAAAGC CATCGACGAT
    GGCGCTGAGT TCGACGAGGA AGAGGAGGAA GACGATTCGA AGCGCAAGCG ACGAAAGCGT
    AAGAATCGGA AGGAAGAGTC CGACGATGAT TCGTTGATCC TAAAACGGCG ACGACGCCAA
    AATCTGGACA AGCGGTCCAA GAAGCAGATG CACAAGATTA TGAGTGCGGT TATCAAGCAC
    AACCAAGATG GACGAACCCT GTCCGAACCG TTCATGAAGT TGCCTTCGCG GCAGAGGTTG
    CCCGACTACT ACGAGATCAT TAAGCGGCCA GTTGATATTA AGAAAATCCT GCAGCGCATT
    GAGGACTGCA AGTATGCCGA TCTCAACGAG CTGGAGAAGG ACTTTATGCA GCTATGCCAG
    AATGCACAGA TCTACAACGA AGAGGCGTCT CTGATTTACC TGGACTCGAT TGCCCTGCAA
    AAGGTATTCG TTGGGGCCAG GCAGAGGATA ACAGCTGCAG CGGATGCAGC CGCAGTGGCA
    GCCGGAGATA ACACGGGTGA AGCGCACGGC AATGGAGGTT CGGATAATTC GGACAACGAC
    GACGACGATG GTGGCGATGA CGGCTCCGAC GACGAGGAGA TTGCAACCAC TTCAGCGGCG
    GCTGTCAAAA TGAAGCTGAA ACTGAACAAA TCTCTGGCTA GTGCGCCCGC TACGCCCACG
    CAATCATCGT CGAATGTGAG CAGCGGAGCG GCTACCACAT CCAAGAAGCA GACGCGTCGC
    AAGCGCTCCC AAAAGAAGTA CACTATTTCA GACGATGACG ACGATGACAT GGACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.