brm cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	brm
Entrez Gene ID	39744
Full Name	brahma
Synonyms	BRD,BRM,Brahma,Brm,Brm @ Kto,CG18438,CG5942,Dmel\CG5942,E(E2F)3A,dBRM,dSMARCA4,dSNF2,l(3)72Aa
General protein information	Preferred Names brahma Names CG5942 gene product from transcript CG5942-RD CG5942-PA CG5942-PB CG5942-PC CG5942-PD CG5942-PE CG5942-PF brm-PA brm-PB brm-PC brm-PD brm-PE brm-PF complementation group 3.5
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 72C1-72C1\|3-43.0 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001274977.1	NP_001261906.1	brahma, isoform E
NM_168641.1	NP_730089.1	brahma, isoform B
NM_168640.2	NP_730088.1	brahma, isoform D
NM_080498.3	NP_536746.1	brahma, isoform A
NM_001274978.1	NP_001261907.1	brahma, isoform F
NM_080497.5	NP_536745.4	brahma, isoform C

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Smarca2	NP_035546.2
Rattus norvegicus (Norway rat)	Smarca2	NP_001004446.1
Saccharomyces cerevisiae (baker's yeast)	STH1	NP_012140.1
Schizosaccharomyces pombe (fission yeast)	snf22	NP_588472.2
Gallus gallus (chicken)	SMARCA2	NP_990470.1
Arabidopsis thaliana (thale cress)	AT5G19310	NP_197432.2
Arabidopsis thaliana (thale cress)	ATCHR12	NP_187252.2
Canis lupus familiaris (dog)	SMARCA2	XP_850159.1
Danio rerio (zebrafish)	smarca2	NP_001038240.1
Eremothecium gossypii	AGOS_AER375C	NP_985231.1
Schizosaccharomyces pombe (fission yeast)	snf21	NP_594861.1
Magnaporthe oryzae (rice blast fungus)	MGG_06388	XP_003717185.1
Neurospora crassa	NCU06488	XP_957128.1
Xenopus tropicalis (tropical clawed frog)	smarca2	XP_004910846.1
Homo sapiens (human)	SMARCA2	NP_001276325.1
Bos taurus (cattle)	SMARCA2	NP_001092585.1
Drosophila melanogaster (fruit fly)	brm	NP_730089.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010462	XP_311484.3
Pan troglodytes (chimpanzee)	SMARCA2	XP_003951484.1
Caenorhabditis elegans (roundworm)	swsn-4	NP_502082.1
Kluyveromyces lactis	KLLA0F04521g	XP_455284.1
Oryza sativa (rice)	Os05g0144300	NP_001054624.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Examination of the epistatic relationship between candidate genes and Stat92E reveals that Ptp61F and brm interact with Stat92E during both border cell specification and migration.
Title: Identification of Novel Regulators of the JAK/STAT Signaling Pathway that Control Border Cell Migration in the Drosophila Ovary.

This paper identified the Brahma (Brm) complex as a regulator of the Drosophila clock
Title: The Catalytic and Non-catalytic Functions of the Brahma Chromatin-Remodeling Protein Collaborate to Fine-Tune Circadian Transcription in Drosophila.

Brahma functions as a critical regulator of Hippo signaling during tissue growth and plays an important role in the feedback loop between Crb and Yki.
Title: Brahma regulates the Hippo pathway activity through forming complex with Yki-Sd and regulating the transcription of Crumbs.

Study characterizes the BRAHMA (BRM) chromatin-remodeling protein in Drosophila in the context of circadian clock regulation. By dissecting its catalytic vs. non-catalytic activities, we propose a model in which the non-catalytic activity of BRM functions to recruit repressive factors to limit the transcriptional output of CLK during the active phase of circadian transcription.
Title: Understanding the role of chromatin remodeling in the regulation of circadian transcription in Drosophila.

The results show that Brm knockdown leads to nucleosome occupancy changes throughout the entire genome with a bias in occupancy decrease.
Title: Drosophila Brahma complex remodels nucleosome organizations in multiple aspects.

The following brm gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the brm cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa18620	NM_001274978.1 Latest version!	Drosophila melanogaster brahma (brm), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa18621	NM_001274977.1 Latest version!	Drosophila melanogaster brahma (brm), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa20961	NM_168640.2 Latest version!	Drosophila melanogaster brahma (brm), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa20961	NM_080497.5 Latest version!	Drosophila melanogaster brahma (brm), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa20962	NM_080498.3 Latest version!	Drosophila melanogaster brahma (brm), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa20962	NM_168641.1 Latest version!	Drosophila melanogaster brahma (brm), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

brm ( NM_001274978.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261907.1 Drosophila melanogaster brahma (brm), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa18620
Clone ID Related Accession (Same CDS sequence)	NM_001274978.1
Accession Version	NM_001274978.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	brahma, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCTCGC CCTCTCCGGC GAACAGTCCC ATGCCACCGC CACAGGCGCC CAGTCCGATG 
GCCCCGCCCT CGCAGAGTCC CGCCCCCTCG CCGCACAGCC CGTATCCGCA CCAGCAGCCG 
GGTCCACTGC AAGGACCTCC GCCACCCGGA CATCCCGGTG CCTACGGACA TCCCATGCAG 
CACGGACCAC CCGGCCAGGG TCCACCCGGG CATCATATGC CACCGCACCA CCAGGGGATG 
AGTCCGCACA TGGGAATGCA GATGCCGCCG ACGGGACCGA ACATGTCCCC CTACCAAACC 
CACGGAATGC CACCCAATCT TTTGTGCAAA ATGGAATTTG TAGCACCCAC ACAACCCTGC 
ATAGTGTCAC CTGGTGGCCC ACCTGGAGGC CCGCCACCTC CGGAGCGCTC GAGCCAGGAG 
AACCTGCACG CGCTTCAACG CGCCATTGAT TCAATGGAGG AAAAGGGCTT GCAAGAGGAT 
CCTCGCTACT CGCAGCTGCT GGCAATGCGA GCAACCTCGA AGCATCAGCA CCTTAATGGC 
AATCAGGTTA ACTTGTTGCG CACACAGATC ACCGCCTATC GGTTGCTGGC GCGGAACAAG 
CCCATATCCA TGCAGATGCA GCAGGCGTTG CAGGCCGCAC AGCAACAGCC ACCGCCAGGA 
CCTCCAATTG GGCCACCTGG AGCACCAGGC GGACCGCCAC CAGGGAGCCA GCATGCAGGA 
CAACCGCCCG TGCCGCCGCA ACAACAGCAA CAACCGCCAC CTTCAGCAGG AACTCCACCC 
CAGTGCTCCA CGCCGCCCGC AAGCAATCCT TATGGACCGC CAGTTCCTGG CCAGAAAATG 
CAGGTAGCGC CACCACCGCC ACACATGCAA CAGGGACAGC CATTGCCACC ACAACCGCCA 
CAAGTGGGTG GACCCCCGCC TATACAACAG CAGCAACCAC CTCAGCAGCA GCAACAACAG 
TCGCAGCCCC CGCCACCGGA GCCACATCAG CACCAGTTGC CAAACGGAGG CAAACCCCTG 
AGTATGGGGC CATCTGGCGG GCAACCACTA ATACCTTCAT CACCCATGCA GCCACAAGTG 
AGAGGGACAC TGCCAGGAAT GCCGCCAGGT AGTCAGGTTC CGCAACCAGG AGGAGGTCCT 
CAGCGACAGG TACCACCTGC GGGCATGCCG ATGCCCAAGC CCAATCGCAT TACAACGGTG 
GCCAAACCCG TCGGTCTGGA TCCCATAACA TTGCTGCAGG AAAGGGAGAA TAGGATAGCA 
GCCAGGATAT CATTGCGCAT GCAGGAGTTG CAGCGACTGC CGGCCACGAT GTCCGAGGAT 
CTGCGTCTGC AAGCGGCTAT CGAGTTGAGG GCCCTAAGGG TCTTGAACTT CCAGCGACAG 
CTGCGTATGG AGTTTGTTCA GTGCACGAGA CGAGATACCA CTTTGGAAAC AGCGTTAAAC 
ATTAAGCTCT ACAAGAGGAC CAAGAGGCAG GGCCTGCGGG AGGCACGTGC CACCGAGAAG 
CTAGAGAAGC AACAGAAGCT AGAGGCTGAA CGAAAGCGTC GCCAAAAGCA TCTGGAGTTC 
TTGGCTGCTG TGCTTCAGCA CGGCAAGGAT CTAAGGGAGT TCCACAGAAA TAATAAGGCC 
CAGCTTGCAA GGATGAACAA GGCCGTGATG AATCACCACG CCAATGCCGA GCGTGAACAG 
AAGAAGGAAC AAGAGCGCAT CGAAAAGGAA CGTATGCGAC GTCTGATGGC CGAGGATGAG 
GAGGGTTACC GCAAGCTGAT TGATCAAAAG AAGGACAAGA GATTGGCTTT CCTACTGTCG 
CAGACAGATG AGTATATTAG CAACCTAACT CAGATGGTGA AACAGCACAA GGACGACCAG 
ATGAAGAAAA AGGAGGAGGA GGGCAAGCGG CTGATACAGT TCAAAAAGGA GCTGCTGATG 
AGCGGAGAGT ACATTGGCAT TGATGAGGGC AGCATTGTTG CCGATATGCG GGTACATGTG 
GTGGAACAGT GCACGGGTAA GAAACTCACC GGCGATGATG CACCCATGCT GAAGCACTTG 
CACCGCTGGC TGAATATGCA TCCTGGCTGG GATTGGATCG ACGACGAGGA GGACAGCTGT 
GGGAGCAACG ACGATCATAA ACCCAAGGTG GAGGAGCAAC CAACGGCAAC AGAAGATGCC 
ACCGACAAAG CGCAGGCGAC GGGCAACGAT GAGGATGCCA AGGATCTAAT TACCAAGGCC 
AAGGTGGAGG ACGATGAGTA CAGGACGGAG GAACAGACGT ACTACAGCAT CGCTCATACC 
ATTCATGAAA AGGTTGTGGA GCAGGCCAGC ATCATGGTTA ATGGTACGCT CAAGGAATAC 
CAGATCAAAG GCTTGGAGTG GTTGGTTTCG CTGTACAATA ACAATCTAAA TGGTATTTTG 
GCCGATGAAA TGGGTTTGGG TAAAACCATT CAAACCATTT CGCTGGTAAC CTACCTTATG 
GATCGAAAGA AGGTTATGGG TCCTTACTTG ATTATTGTAC CACTCTCCAC CCTGCCCAAT 
TGGGTGCTGG AATTCGAGAA GTGGGCGCCA GCCGTGGGTG TAGTCAGCTA CAAGGGCAGT 
CCGCAGGGAA GGCGATTGCT GCAAAATCAA ATGAGAGCAA CCAAGTTCAA TGTGCTGTTA 
ACCACATATG AGTACGTGAT TAAGGACAAG GCGGTGCTGG CCAAGATCCA GTGGAAATAC 
ATGATTATCG ACGAGGGTCA CCGCATGAAG AACCATCACT GCAAACTCAC CCAGGTGCTG 
AACACCCACT ACATTGCCCC CTATCGGTTA CTTCTCACGG GTACACCTCT GCAAAACAAG 
CTTCCCGAGT TGTGGGCGCT ACTTAATTTC CTGCTGCCCT CGATCTTTAA GTCCTGCTCC 
ACATTTGAAC AGTGGTTCAA TGCGCCATTT GCCACCACTG GCGAGAAGGT CGAGCTAAAC 
GAGGAGGAGA CCATCCTTAT CATTCGTCGT CTGCACAAGG TTCTTCGTCC TTTCCTGTTG 
CGTCGCCTCA AAAAGGAGGT AGAGCACCAG CTGCCCGACA AGGTCGAGTA CATCATCAAG 
TGCGACATGT CCGCGCTGCA ACGCGTTCTC TACAAGCACA TGCAGAGCAA GGGTGTCCTG 
CTCACCGATG GTTCCGAGAA GGGCAAGCAC GGCAAGGGAG GCGCTAAGGC GCTGATGAAC 
ACGATCGTAC AGCTGCGCAA GCTGTGCAAC CATCCGTTTA TGTTCCAGCA CATAGAAGAG 
AAGTACTGCG ATCACACTGG CGGCCACGGA GTTGTATCCG GACCGGATCT GTATCGTGTC 
TCTGGCAAAT TCGAGCTGCT TGATCGCATC CTGCCCAAGC TGAAGGCGAC CAATCATCGC 
GTGCTGCTCT TCTGTCAGAT GACCCAATGC ATGACCATCA TCGAAGACTA CCTGGGATGG 
CGCCAATTCG GCTACCTTCG ACTCGACGGT ACCACGAAGG CCGAGGATCG TGGCGAACTA 
CTGCGAAAAT TCAACGCCAA AGGCTCGGAT GTATTTGTCT TTTTGCTATC TACCCGAGCT 
GGTGGTTTGG GTCTTAATCT GCAGACCGCC GATACTGTAG TAATCTTCGA CTCCGATTGG 
AATCCCCATC AGGATCTGCA AGCCCAGGAT CGTGCCCATC GTATTGGTCA ACGTAACGAG 
GTGCGCGTCC TGCGACTAAT GACAGTCAAC TCGGTGGAAG AGCGCATCTT GGCAGCAGCC 
AGGTACAAGC TGAACATGGA CGAGAAGGTC ATCCAGGCTG GTATGTTCGA TCAGAAGTCA 
ACGGGTAGCG AGCGACAGCA GTTCCTACAA ACGATTCTGC ACCAGGATGA CAACGAAGAG 
GAGGAGGAAA ATGAGGTGCC CGATGACGAA ATGATCAACA TGATGATCGC CCGCAGCGAG 
GAGGAGATTG AAATATTTAA GCGCATGGAT GCTGAGCGCA AGAAGGAGGA TGAGGAGATC 
CATCCGGGTA GGGAACGTCT GATCGATGAG TCCGAGCTAC CCGACTGGTT GACAAAGGAT 
GATGACGAAG TAGAGCGTTT CCACTATCAG TACGACGAGG ATACCATTCT AGGTCGAGGC 
TCCCGGCAGC GAAAGGAAGT AGACTACACC GATAGTTTGA CTGAAAAGGA GTGGCTGAAA 
GCCATCGACG ATGGCGCTGA GTTCGACGAG GAAGAGGAGG AAGACGATTC GAAGCGCAAG 
CGACGAAAGC GTAAGAATCG GAAGGAAGAG TCCGACGATG ATTCGTTGAT CCTAAAACGG 
CGACGACGCC AAAATCTGGA CAAGCGGTCC AAGAAGCAGA TGCACAAGAT TATGAGTGCG 
GTTATCAAGC ACAACCAAGA TGGACGAACC CTGTCCGAAC CGTTCATGAA GTTGCCTTCG 
CGGCAGAGGT TGCCCGACTA CTACGAGATC ATTAAGCGGC CAGTTGATAT TAAGAAAATC 
CTGCAGCGCA TTGAGGACTG CAAGTATGCC GATCTCAACG AGCTGGAGAA GGACTTTATG 
CAGCTATGCC AGAATGCACA GATCTACAAC GAAGAGGCGT CTCTGATTTA CCTGGACTCG 
ATTGCCCTGC AAAAGGTATT CGTTGGGGCC AGGCAGAGGA TAACAGCTGC AGCGGATGCA 
GCCGCAGTGG CAGCCGGAGA TAACACGGGT GAAGCGCACG GCAATGGAGG TTCGGATAAT 
TCGGACAACG ACGACGACGA TGGTGGCGAT GACGGCTCCG ACGACGAGGA GATTGCAACC 
ACTTCAGCGG CGGCTGTCAA AATGAAGCTG AAACTGAACA AATCTCTGGC TAGTGCGCCC 
GCTACGCCCA CGCAATCATC GTCGAATGTG AGCAGCGGAG CGGCTACCAC ATCCAAGAAG 
CAGACGCGTC GCAAGCGCTC CCAAAAGAAG TACACTATTT CAGACGATGA CGACGATGAC 
ATGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261907.1
CDS	128..5056
Translation	MASPSPANSPMPPPQAPSPMAPPSQSPAPSPHSPYPHQQPGPLQGPPPPGHPGAYGHPMQHGPPGQGPPGHHMPPHHQGMSPHMGMQMPPTGPNMSPYQTHGMPPNLLCKMEFVAPTQPCIVSPGGPPGGPPPPERSSQENLHALQRAIDSMEEKGLQEDPRYSQLLAMRATSKHQHLNGNQVNLLRTQITAYRLLARNKPISMQMQQALQAAQQQPPPGPPIGPPGAPGGPPPGSQHAGQPPVPPQQQQQPPPSAGTPPQCSTPPASNPYGPPVPGQKMQVAPPPPHMQQGQPLPPQPPQVGGPPPIQQQQPPQQQQQQSQPPPPEPHQHQLPNGGKPLSMGPSGGQPLIPSSPMQPQVRGTLPGMPPGSQVPQPGGGPQRQVPPAGMPMPKPNRITTVAKPVGLDPITLLQERENRIAARISLRMQELQRLPATMSEDLRLQAAIELRALRVLNFQRQLRMEFVQCTRRDTTLETALNIKLYKRTKRQGLREARATEKLEKQQKLEAERKRRQKHLEFLAAVLQHGKDLREFHRNNKAQLARMNKAVMNHHANAEREQKKEQERIEKERMRRLMAEDEEGYRKLIDQKKDKRLAFLLSQTDEYISNLTQMVKQHKDDQMKKKEEEGKRLIQFKKELLMSGEYIGIDEGSIVADMRVHVVEQCTGKKLTGDDAPMLKHLHRWLNMHPGWDWIDDEEDSCGSNDDHKPKVEEQPTATEDATDKAQATGNDEDAKDLITKAKVEDDEYRTEEQTYYSIAHTIHEKVVEQASIMVNGTLKEYQIKGLEWLVSLYNNNLNGILADEMGLGKTIQTISLVTYLMDRKKVMGPYLIIVPLSTLPNWVLEFEKWAPAVGVVSYKGSPQGRRLLQNQMRATKFNVLLTTYEYVIKDKAVLAKIQWKYMIIDEGHRMKNHHCKLTQVLNTHYIAPYRLLLTGTPLQNKLPELWALLNFLLPSIFKSCSTFEQWFNAPFATTGEKVELNEEETILIIRRLHKVLRPFLLRRLKKEVEHQLPDKVEYIIKCDMSALQRVLYKHMQSKGVLLTDGSEKGKHGKGGAKALMNTIVQLRKLCNHPFMFQHIEEKYCDHTGGHGVVSGPDLYRVSGKFELLDRILPKLKATNHRVLLFCQMTQCMTIIEDYLGWRQFGYLRLDGTTKAEDRGELLRKFNAKGSDVFVFLLSTRAGGLGLNLQTADTVVIFDSDWNPHQDLQAQDRAHRIGQRNEVRVLRLMTVNSVEERILAAARYKLNMDEKVIQAGMFDQKSTGSERQQFLQTILHQDDNEEEEENEVPDDEMINMMIARSEEEIEIFKRMDAERKKEDEEIHPGRERLIDESELPDWLTKDDDEVERFHYQYDEDTILGRGSRQRKEVDYTDSLTEKEWLKAIDDGAEFDEEEEEDDSKRKRRKRKNRKEESDDDSLILKRRRRQNLDKRSKKQMHKIMSAVIKHNQDGRTLSEPFMKLPSRQRLPDYYEIIKRPVDIKKILQRIEDCKYADLNELEKDFMQLCQNAQIYNEEASLIYLDSIALQKVFVGARQRITAAADAAAVAAGDNTGEAHGNGGSDNSDNDDDDGGDDGSDDEEIATTSAAAVKMKLKLNKSLASAPATPTQSSSNVSSGAATTSKKQTRRKRSQKKYTISDDDDDDMD

Target ORF information:

RefSeq Version	NM_001274978.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster brahma (brm), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274978.1

ORF Insert Sequence:

ATGGCCTCGC CCTCTCCGGC GAACAGTCCC ATGCCACCGC CACAGGCGCC CAGTCCGATG 
GCCCCGCCCT CGCAGAGTCC CGCCCCCTCG CCGCACAGCC CGTATCCGCA CCAGCAGCCG 
GGTCCACTGC AAGGACCTCC GCCACCCGGA CATCCCGGTG CCTACGGACA TCCCATGCAG 
CACGGACCAC CCGGCCAGGG TCCACCCGGG CATCATATGC CACCGCACCA CCAGGGGATG 
AGTCCGCACA TGGGAATGCA GATGCCGCCG ACGGGACCGA ACATGTCCCC CTACCAAACC 
CACGGAATGC CACCCAATCT TTTGTGCAAA ATGGAATTTG TAGCACCCAC ACAACCCTGC 
ATAGTGTCAC CTGGTGGCCC ACCTGGAGGC CCGCCACCTC CGGAGCGCTC GAGCCAGGAG 
AACCTGCACG CGCTTCAACG CGCCATTGAT TCAATGGAGG AAAAGGGCTT GCAAGAGGAT 
CCTCGCTACT CGCAGCTGCT GGCAATGCGA GCAACCTCGA AGCATCAGCA CCTTAATGGC 
AATCAGGTTA ACTTGTTGCG CACACAGATC ACCGCCTATC GGTTGCTGGC GCGGAACAAG 
CCCATATCCA TGCAGATGCA GCAGGCGTTG CAGGCCGCAC AGCAACAGCC ACCGCCAGGA 
CCTCCAATTG GGCCACCTGG AGCACCAGGC GGACCGCCAC CAGGGAGCCA GCATGCAGGA 
CAACCGCCCG TGCCGCCGCA ACAACAGCAA CAACCGCCAC CTTCAGCAGG AACTCCACCC 
CAGTGCTCCA CGCCGCCCGC AAGCAATCCT TATGGACCGC CAGTTCCTGG CCAGAAAATG 
CAGGTAGCGC CACCACCGCC ACACATGCAA CAGGGACAGC CATTGCCACC ACAACCGCCA 
CAAGTGGGTG GACCCCCGCC TATACAACAG CAGCAACCAC CTCAGCAGCA GCAACAACAG 
TCGCAGCCCC CGCCACCGGA GCCACATCAG CACCAGTTGC CAAACGGAGG CAAACCCCTG 
AGTATGGGGC CATCTGGCGG GCAACCACTA ATACCTTCAT CACCCATGCA GCCACAAGTG 
AGAGGGACAC TGCCAGGAAT GCCGCCAGGT AGTCAGGTTC CGCAACCAGG AGGAGGTCCT 
CAGCGACAGG TACCACCTGC GGGCATGCCG ATGCCCAAGC CCAATCGCAT TACAACGGTG 
GCCAAACCCG TCGGTCTGGA TCCCATAACA TTGCTGCAGG AAAGGGAGAA TAGGATAGCA 
GCCAGGATAT CATTGCGCAT GCAGGAGTTG CAGCGACTGC CGGCCACGAT GTCCGAGGAT 
CTGCGTCTGC AAGCGGCTAT CGAGTTGAGG GCCCTAAGGG TCTTGAACTT CCAGCGACAG 
CTGCGTATGG AGTTTGTTCA GTGCACGAGA CGAGATACCA CTTTGGAAAC AGCGTTAAAC 
ATTAAGCTCT ACAAGAGGAC CAAGAGGCAG GGCCTGCGGG AGGCACGTGC CACCGAGAAG 
CTAGAGAAGC AACAGAAGCT AGAGGCTGAA CGAAAGCGTC GCCAAAAGCA TCTGGAGTTC 
TTGGCTGCTG TGCTTCAGCA CGGCAAGGAT CTAAGGGAGT TCCACAGAAA TAATAAGGCC 
CAGCTTGCAA GGATGAACAA GGCCGTGATG AATCACCACG CCAATGCCGA GCGTGAACAG 
AAGAAGGAAC AAGAGCGCAT CGAAAAGGAA CGTATGCGAC GTCTGATGGC CGAGGATGAG 
GAGGGTTACC GCAAGCTGAT TGATCAAAAG AAGGACAAGA GATTGGCTTT CCTACTGTCG 
CAGACAGATG AGTATATTAG CAACCTAACT CAGATGGTGA AACAGCACAA GGACGACCAG 
ATGAAGAAAA AGGAGGAGGA GGGCAAGCGG CTGATACAGT TCAAAAAGGA GCTGCTGATG 
AGCGGAGAGT ACATTGGCAT TGATGAGGGC AGCATTGTTG CCGATATGCG GGTACATGTG 
GTGGAACAGT GCACGGGTAA GAAACTCACC GGCGATGATG CACCCATGCT GAAGCACTTG 
CACCGCTGGC TGAATATGCA TCCTGGCTGG GATTGGATCG ACGACGAGGA GGACAGCTGT 
GGGAGCAACG ACGATCATAA ACCCAAGGTG GAGGAGCAAC CAACGGCAAC AGAAGATGCC 
ACCGACAAAG CGCAGGCGAC GGGCAACGAT GAGGATGCCA AGGATCTAAT TACCAAGGCC 
AAGGTGGAGG ACGATGAGTA CAGGACGGAG GAACAGACGT ACTACAGCAT CGCTCATACC 
ATTCATGAAA AGGTTGTGGA GCAGGCCAGC ATCATGGTTA ATGGTACGCT CAAGGAATAC 
CAGATCAAAG GCTTGGAGTG GTTGGTTTCG CTGTACAATA ACAATCTAAA TGGTATTTTG 
GCCGATGAAA TGGGTTTGGG TAAAACCATT CAAACCATTT CGCTGGTAAC CTACCTTATG 
GATCGAAAGA AGGTTATGGG TCCTTACTTG ATTATTGTAC CACTCTCCAC CCTGCCCAAT 
TGGGTGCTGG AATTCGAGAA GTGGGCGCCA GCCGTGGGTG TAGTCAGCTA CAAGGGCAGT 
CCGCAGGGAA GGCGATTGCT GCAAAATCAA ATGAGAGCAA CCAAGTTCAA TGTGCTGTTA 
ACCACATATG AGTACGTGAT TAAGGACAAG GCGGTGCTGG CCAAGATCCA GTGGAAATAC 
ATGATTATCG ACGAGGGTCA CCGCATGAAG AACCATCACT GCAAACTCAC CCAGGTGCTG 
AACACCCACT ACATTGCCCC CTATCGGTTA CTTCTCACGG GTACACCTCT GCAAAACAAG 
CTTCCCGAGT TGTGGGCGCT ACTTAATTTC CTGCTGCCCT CGATCTTTAA GTCCTGCTCC 
ACATTTGAAC AGTGGTTCAA TGCGCCATTT GCCACCACTG GCGAGAAGGT CGAGCTAAAC 
GAGGAGGAGA CCATCCTTAT CATTCGTCGT CTGCACAAGG TTCTTCGTCC TTTCCTGTTG 
CGTCGCCTCA AAAAGGAGGT AGAGCACCAG CTGCCCGACA AGGTCGAGTA CATCATCAAG 
TGCGACATGT CCGCGCTGCA ACGCGTTCTC TACAAGCACA TGCAGAGCAA GGGTGTCCTG 
CTCACCGATG GTTCCGAGAA GGGCAAGCAC GGCAAGGGAG GCGCTAAGGC GCTGATGAAC 
ACGATCGTAC AGCTGCGCAA GCTGTGCAAC CATCCGTTTA TGTTCCAGCA CATAGAAGAG 
AAGTACTGCG ATCACACTGG CGGCCACGGA GTTGTATCCG GACCGGATCT GTATCGTGTC 
TCTGGCAAAT TCGAGCTGCT TGATCGCATC CTGCCCAAGC TGAAGGCGAC CAATCATCGC 
GTGCTGCTCT TCTGTCAGAT GACCCAATGC ATGACCATCA TCGAAGACTA CCTGGGATGG 
CGCCAATTCG GCTACCTTCG ACTCGACGGT ACCACGAAGG CCGAGGATCG TGGCGAACTA 
CTGCGAAAAT TCAACGCCAA AGGCTCGGAT GTATTTGTCT TTTTGCTATC TACCCGAGCT 
GGTGGTTTGG GTCTTAATCT GCAGACCGCC GATACTGTAG TAATCTTCGA CTCCGATTGG 
AATCCCCATC AGGATCTGCA AGCCCAGGAT CGTGCCCATC GTATTGGTCA ACGTAACGAG 
GTGCGCGTCC TGCGACTAAT GACAGTCAAC TCGGTGGAAG AGCGCATCTT GGCAGCAGCC 
AGGTACAAGC TGAACATGGA CGAGAAGGTC ATCCAGGCTG GTATGTTCGA TCAGAAGTCA 
ACGGGTAGCG AGCGACAGCA GTTCCTACAA ACGATTCTGC ACCAGGATGA CAACGAAGAG 
GAGGAGGAAA ATGAGGTGCC CGATGACGAA ATGATCAACA TGATGATCGC CCGCAGCGAG 
GAGGAGATTG AAATATTTAA GCGCATGGAT GCTGAGCGCA AGAAGGAGGA TGAGGAGATC 
CATCCGGGTA GGGAACGTCT GATCGATGAG TCCGAGCTAC CCGACTGGTT GACAAAGGAT 
GATGACGAAG TAGAGCGTTT CCACTATCAG TACGACGAGG ATACCATTCT AGGTCGAGGC 
TCCCGGCAGC GAAAGGAAGT AGACTACACC GATAGTTTGA CTGAAAAGGA GTGGCTGAAA 
GCCATCGACG ATGGCGCTGA GTTCGACGAG GAAGAGGAGG AAGACGATTC GAAGCGCAAG 
CGACGAAAGC GTAAGAATCG GAAGGAAGAG TCCGACGATG ATTCGTTGAT CCTAAAACGG 
CGACGACGCC AAAATCTGGA CAAGCGGTCC AAGAAGCAGA TGCACAAGAT TATGAGTGCG 
GTTATCAAGC ACAACCAAGA TGGACGAACC CTGTCCGAAC CGTTCATGAA GTTGCCTTCG 
CGGCAGAGGT TGCCCGACTA CTACGAGATC ATTAAGCGGC CAGTTGATAT TAAGAAAATC 
CTGCAGCGCA TTGAGGACTG CAAGTATGCC GATCTCAACG AGCTGGAGAA GGACTTTATG 
CAGCTATGCC AGAATGCACA GATCTACAAC GAAGAGGCGT CTCTGATTTA CCTGGACTCG 
ATTGCCCTGC AAAAGGTATT CGTTGGGGCC AGGCAGAGGA TAACAGCTGC AGCGGATGCA 
GCCGCAGTGG CAGCCGGAGA TAACACGGGT GAAGCGCACG GCAATGGAGG TTCGGATAAT 
TCGGACAACG ACGACGACGA TGGTGGCGAT GACGGCTCCG ACGACGAGGA GATTGCAACC 
ACTTCAGCGG CGGCTGTCAA AATGAAGCTG AAACTGAACA AATCTCTGGC TAGTGCGCCC 
GCTACGCCCA CGCAATCATC GTCGAATGTG AGCAGCGGAG CGGCTACCAC ATCCAAGAAG 
CAGACGCGTC GCAAGCGCTC CCAAAAGAAG TACACTATTT CAGACGATGA CGACGATGAC 
ATGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

brm ( NM_001274977.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261906.1 Drosophila melanogaster brahma (brm), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa18621
Clone ID Related Accession (Same CDS sequence)	NM_001274977.1
Accession Version	NM_001274977.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	brahma, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCTCGC CCTCTCCGGC GAACAGTCCC ATGCCACCGC CACAGGCGCC CAGTCCGATG 
GCCCCGCCCT CGCAGAGTCC CGCCCCCTCG CCGCACAGCC CGTATCCGCA CCAGCAGCCG 
GGTCCACTGC AAGGACCTCC GCCACCCGGA CATCCCGGTG CCTACGGACA TCCCATGCAG 
CACGGACCAC CCGGCCAGGG TCCACCCGGG CATCATATGC CACCGCACCA CCAGGGGATG 
ATTTTCTCAA AAGGTCCGCA CATGGGAATG CAGATGCCGC CGACGGGACC GAACATGTCC 
CCCTACCAAA CCCACGGAAT GCCACCCAAT GCACCCACAC AACCCTGCAT AGTGTCACCT 
GGTGGCCCAC CTGGAGGCCC GCCACCTCCG GAGCGCTCGA GCCAGGAGAA CCTGCACGCG 
CTTCAACGCG CCATTGATTC AATGGAGGAA AAGGGCTTGC AAGAGGATCC TCGCTACTCG 
CAGCTGCTGG CAATGCGAGC AACCTCGAAG CATCAGCACC TTAATGGCAA TCAGGTTAAC 
TTGTTGCGCA CACAGATCAC CGCCTATCGG TTGCTGGCGC GGAACAAGCC CATATCCATG 
CAGATGCAGC AGGCGTTGCA GGCCGCACAG CAACAGCCAC CGCCAGGACC TCCAATTGGG 
CCACCTGGAG CACCAGGCGG ACCGCCACCA GGGAGCCAGC ATGCAGGACA ACCGCCCGTG 
CCGCCGCAAC AACAGCAACA ACCGCCACCT TCAGCAGGAA CTCCACCCCA GTGCTCCACG 
CCGCCCGCAA GCAATCCTTA TGGACCGCCA GTTCCTGGCC AGAAAATGCA GGTAGCGCCA 
CCACCGCCAC ACATGCAACA GGGACAGCCA TTGCCACCAC AACCGCCACA AGTGGGTGGA 
CCCCCGCCTA TACAACAGCA GCAACCACCT CAGCAGCAGC AACAACAGTC GCAGCCCCCG 
CCACCGGAGC CACATCAGCA CCAGTTGCCA AACGGAGGCA AACCCCTGAG TATGGGGCCA 
TCTGGCGGGC AACCACTAAT ACCTTCATCA CCCATGCAGC CACAAGTGAG AGGGACACTG 
CCAGGAATGC CGCCAGGTAG TCAGGTTCCG CAACCAGGAG GAGGTCCTCA GCGACAGGTA 
CCACCTGCGG GCATGCCGAT GCCCAAGCCC AATCGCATTA CAACGGTGGC CAAACCCGTC 
GGTCTGGATC CCATAACATT GCTGCAGGAA AGGGAGAATA GGATAGCAGC CAGGATATCA 
TTGCGCATGC AGGAGTTGCA GCGACTGCCG GCCACGATGT CCGAGGATCT GCGTCTGCAA 
GCGGCTATCG AGTTGAGGGC CCTAAGGGTC TTGAACTTCC AGCGACAGCT GCGTATGGAG 
TTTGTTCAGT GCACGAGACG AGATACCACT TTGGAAACAG CGTTAAACAT TAAGCTCTAC 
AAGAGGACCA AGAGGCAGGG CCTGCGGGAG GCACGTGCCA CCGAGAAGCT AGAGAAGCAA 
CAGAAGCTAG AGGCTGAACG AAAGCGTCGC CAAAAGCATC TGGAGTTCTT GGCTGCTGTG 
CTTCAGCACG GCAAGGATCT AAGGGAGTTC CACAGAAATA ATAAGGCCCA GCTTGCAAGG 
ATGAACAAGG CCGTGATGAA TCACCACGCC AATGCCGAGC GTGAACAGAA GAAGGAACAA 
GAGCGCATCG AAAAGGAACG TATGCGACGT CTGATGGCCG AGGATGAGGA GGGTTACCGC 
AAGCTGATTG ATCAAAAGAA GGACAAGAGA TTGGCTTTCC TACTGTCGCA GACAGATGAG 
TATATTAGCA ACCTAACTCA GATGGTGAAA CAGCACAAGG ACGACCAGAT GAAGAAAAAG 
GAGGAGGAGG GCAAGCGGCT GATACAGTTC AAAAAGGAGC TGCTGATGAG CGGAGAGTAC 
ATTGGCATTG ATGAGGGCAG CATTGTTGCC GATATGCGGG TACATGTGGT GGAACAGTGC 
ACGGGTAAGA AACTCACCGG CGATGATGCA CCCATGCTGA AGCACTTGCA CCGCTGGCTG 
AATATGCATC CTGGCTGGGA TTGGATCGAC GACGAGGAGG ACAGCTGTGG GAGCAACGAC 
GATCATAAAC CCAAGGTGGA GGAGCAACCA ACGGCAACAG AAGATGCCAC CGACAAAGCG 
CAGGCGACGG GCAACGATGA GGATGCCAAG GATCTAATTA CCAAGGCCAA GGTGGAGGAC 
GATGAGTACA GGACGGAGGA ACAGACGTAC TACAGCATCG CTCATACCAT TCATGAAAAG 
GTTGTGGAGC AGGCCAGCAT CATGGTTAAT GGTACGCTCA AGGAATACCA GATCAAAGGC 
TTGGAGTGGT TGGTTTCGCT GTACAATAAC AATCTAAATG GTATTTTGGC CGATGAAATG 
GGTTTGGGTA AAACCATTCA AACCATTTCG CTGGTAACCT ACCTTATGGA TCGAAAGAAG 
GTTATGGGTC CTTACTTGAT TATTGTACCA CTCTCCACCC TGCCCAATTG GGTGCTGGAA 
TTCGAGAAGT GGGCGCCAGC CGTGGGTGTA GTCAGCTACA AGGGCAGTCC GCAGGGAAGG 
CGATTGCTGC AAAATCAAAT GAGAGCAACC AAGTTCAATG TGCTGTTAAC CACATATGAG 
TACGTGATTA AGGACAAGGC GGTGCTGGCC AAGATCCAGT GGAAATACAT GATTATCGAC 
GAGGGTCACC GCATGAAGAA CCATCACTGC AAACTCACCC AGGTGCTGAA CACCCACTAC 
ATTGCCCCCT ATCGGTTACT TCTCACGGGT ACACCTCTGC AAAACAAGCT TCCCGAGTTG 
TGGGCGCTAC TTAATTTCCT GCTGCCCTCG ATCTTTAAGT CCTGCTCCAC ATTTGAACAG 
TGGTTCAATG CGCCATTTGC CACCACTGGC GAGAAGGTCG AGCTAAACGA GGAGGAGACC 
ATCCTTATCA TTCGTCGTCT GCACAAGGTT CTTCGTCCTT TCCTGTTGCG TCGCCTCAAA 
AAGGAGGTAG AGCACCAGCT GCCCGACAAG GTCGAGTACA TCATCAAGTG CGACATGTCC 
GCGCTGCAAC GCGTTCTCTA CAAGCACATG CAGAGCAAGG GTGTCCTGCT CACCGATGGT 
TCCGAGAAGG GCAAGCACGG CAAGGGAGGC GCTAAGGCGC TGATGAACAC GATCGTACAG 
CTGCGCAAGC TGTGCAACCA TCCGTTTATG TTCCAGCACA TAGAAGAGAA GTACTGCGAT 
CACACTGGCG GCCACGGAGT TGTATCCGGA CCGGATCTGT ATCGTGTCTC TGGCAAATTC 
GAGCTGCTTG ATCGCATCCT GCCCAAGCTG AAGGCGACCA ATCATCGCGT GCTGCTCTTC 
TGTCAGATGA CCCAATGCAT GACCATCATC GAAGACTACC TGGGATGGCG CCAATTCGGC 
TACCTTCGAC TCGACGGTAC CACGAAGGCC GAGGATCGTG GCGAACTACT GCGAAAATTC 
AACGCCAAAG GCTCGGATGT ATTTGTCTTT TTGCTATCTA CCCGAGCTGG TGGTTTGGGT 
CTTAATCTGC AGACCGCCGA TACTGTAGTA ATCTTCGACT CCGATTGGAA TCCCCATCAG 
GATCTGCAAG CCCAGGATCG TGCCCATCGT ATTGGTCAAC GTAACGAGGT GCGCGTCCTG 
CGACTAATGA CAGTCAACTC GGTGGAAGAG CGCATCTTGG CAGCAGCCAG GTACAAGCTG 
AACATGGACG AGAAGGTCAT CCAGGCTGGT ATGTTCGATC AGAAGTCAAC GGGTAGCGAG 
CGACAGCAGT TCCTACAAAC GATTCTGCAC CAGGATGACA ACGAAGAGGA GGAGGAAAAT 
GAGGTGCCCG ATGACGAAAT GATCAACATG ATGATCGCCC GCAGCGAGGA GGAGATTGAA 
ATATTTAAGC GCATGGATGC TGAGCGCAAG AAGGAGGATG AGGAGATCCA TCCGGGTAGG 
GAACGTCTGA TCGATGAGTC CGAGCTACCC GACTGGTTGA CAAAGGATGA TGACGAAGTA 
GAGCGTTTCC ACTATCAGTA CGACGAGGAT ACCATTCTAG GTCGAGGCTC CCGGCAGCGA 
AAGGAAGTAG ACTACACCGA TAGTTTGACT GAAAAGGAGT GGCTGAAAGC CATCGACGAT 
GGCGCTGAGT TCGACGAGGA AGAGGAGGAA GACGATTCGA AGCGCAAGCG ACGAAAGCGT 
AAGAATCGGA AGGAAGAGTC CGACGATGAT TCGTTGATCC TAAAACGGCG ACGACGCCAA 
AATCTGGACA AGCGGTCCAA GAAGCAGATG CACAAGATTA TGAGTGCGGT TATCAAGCAC 
AACCAAGATG GACGAACCCT GTCCGAACCG TTCATGAAGT TGCCTTCGCG GCAGAGGTTG 
CCCGACTACT ACGAGATCAT TAAGCGGCCA GTTGATATTA AGAAAATCCT GCAGCGCATT 
GAGGACTGCA AGTATGCCGA TCTCAACGAG CTGGAGAAGG ACTTTATGCA GCTATGCCAG 
AATGCACAGA TCTACAACGA AGAGGCGTCT CTGATTTACC TGGACTCGAT TGCCCTGCAA 
AAGGTATTCG TTGGGGCCAG GCAGAGGATA ACAGCTGCAG CGGATGCAGC CGCAGTGGCA 
GCCGGAGATA ACACGGGTGA AGCGCACGGC AATGGAGGTT CGGATAATTC GGACAACGAC 
GACGACGATG GTGGCGATGA CGGCTCCGAC GACGAGGAGA TTGCAACCAC TTCAGCGGCG 
GCTGTCAAAA TGAAGCTGAA ACTGAACAAA TCTCTGGCTA GTGCGCCCGC TACGCCCACG 
CAATCATCGT CGAATGTGAG CAGCGGAGCG GCTACCACAT CCAAGAAGCA GACGCGTCGC 
AAGCGCTCCC AAAAGAAGTA CACTATTTCA GACGATGACG ACGATGACAT GGACTAGCTA 
CCGTTGGGAG TGATCTTCAA GCGCGGATCG TCGGATGTCG AAGGCATGGG CAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261906.1
CDS	128..5104
Translation	MASPSPANSPMPPPQAPSPMAPPSQSPAPSPHSPYPHQQPGPLQGPPPPGHPGAYGHPMQHGPPGQGPPGHHMPPHHQGMIFSKGPHMGMQMPPTGPNMSPYQTHGMPPNAPTQPCIVSPGGPPGGPPPPERSSQENLHALQRAIDSMEEKGLQEDPRYSQLLAMRATSKHQHLNGNQVNLLRTQITAYRLLARNKPISMQMQQALQAAQQQPPPGPPIGPPGAPGGPPPGSQHAGQPPVPPQQQQQPPPSAGTPPQCSTPPASNPYGPPVPGQKMQVAPPPPHMQQGQPLPPQPPQVGGPPPIQQQQPPQQQQQQSQPPPPEPHQHQLPNGGKPLSMGPSGGQPLIPSSPMQPQVRGTLPGMPPGSQVPQPGGGPQRQVPPAGMPMPKPNRITTVAKPVGLDPITLLQERENRIAARISLRMQELQRLPATMSEDLRLQAAIELRALRVLNFQRQLRMEFVQCTRRDTTLETALNIKLYKRTKRQGLREARATEKLEKQQKLEAERKRRQKHLEFLAAVLQHGKDLREFHRNNKAQLARMNKAVMNHHANAEREQKKEQERIEKERMRRLMAEDEEGYRKLIDQKKDKRLAFLLSQTDEYISNLTQMVKQHKDDQMKKKEEEGKRLIQFKKELLMSGEYIGIDEGSIVADMRVHVVEQCTGKKLTGDDAPMLKHLHRWLNMHPGWDWIDDEEDSCGSNDDHKPKVEEQPTATEDATDKAQATGNDEDAKDLITKAKVEDDEYRTEEQTYYSIAHTIHEKVVEQASIMVNGTLKEYQIKGLEWLVSLYNNNLNGILADEMGLGKTIQTISLVTYLMDRKKVMGPYLIIVPLSTLPNWVLEFEKWAPAVGVVSYKGSPQGRRLLQNQMRATKFNVLLTTYEYVIKDKAVLAKIQWKYMIIDEGHRMKNHHCKLTQVLNTHYIAPYRLLLTGTPLQNKLPELWALLNFLLPSIFKSCSTFEQWFNAPFATTGEKVELNEEETILIIRRLHKVLRPFLLRRLKKEVEHQLPDKVEYIIKCDMSALQRVLYKHMQSKGVLLTDGSEKGKHGKGGAKALMNTIVQLRKLCNHPFMFQHIEEKYCDHTGGHGVVSGPDLYRVSGKFELLDRILPKLKATNHRVLLFCQMTQCMTIIEDYLGWRQFGYLRLDGTTKAEDRGELLRKFNAKGSDVFVFLLSTRAGGLGLNLQTADTVVIFDSDWNPHQDLQAQDRAHRIGQRNEVRVLRLMTVNSVEERILAAARYKLNMDEKVIQAGMFDQKSTGSERQQFLQTILHQDDNEEEEENEVPDDEMINMMIARSEEEIEIFKRMDAERKKEDEEIHPGRERLIDESELPDWLTKDDDEVERFHYQYDEDTILGRGSRQRKEVDYTDSLTEKEWLKAIDDGAEFDEEEEEDDSKRKRRKRKNRKEESDDDSLILKRRRRQNLDKRSKKQMHKIMSAVIKHNQDGRTLSEPFMKLPSRQRLPDYYEIIKRPVDIKKILQRIEDCKYADLNELEKDFMQLCQNAQIYNEEASLIYLDSIALQKVFVGARQRITAAADAAAVAAGDNTGEAHGNGGSDNSDNDDDDGGDDGSDDEEIATTSAAAVKMKLKLNKSLASAPATPTQSSSNVSSGAATTSKKQTRRKRSQKKYTISDDDDDDMD*LPLGVIFKRGSSDVEGMGN

Target ORF information:

RefSeq Version	NM_001274977.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster brahma (brm), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274977.1

ORF Insert Sequence:

ATGGCCTCGC CCTCTCCGGC GAACAGTCCC ATGCCACCGC CACAGGCGCC CAGTCCGATG 
GCCCCGCCCT CGCAGAGTCC CGCCCCCTCG CCGCACAGCC CGTATCCGCA CCAGCAGCCG 
GGTCCACTGC AAGGACCTCC GCCACCCGGA CATCCCGGTG CCTACGGACA TCCCATGCAG 
CACGGACCAC CCGGCCAGGG TCCACCCGGG CATCATATGC CACCGCACCA CCAGGGGATG 
ATTTTCTCAA AAGGTCCGCA CATGGGAATG CAGATGCCGC CGACGGGACC GAACATGTCC 
CCCTACCAAA CCCACGGAAT GCCACCCAAT GCACCCACAC AACCCTGCAT AGTGTCACCT 
GGTGGCCCAC CTGGAGGCCC GCCACCTCCG GAGCGCTCGA GCCAGGAGAA CCTGCACGCG 
CTTCAACGCG CCATTGATTC AATGGAGGAA AAGGGCTTGC AAGAGGATCC TCGCTACTCG 
CAGCTGCTGG CAATGCGAGC AACCTCGAAG CATCAGCACC TTAATGGCAA TCAGGTTAAC 
TTGTTGCGCA CACAGATCAC CGCCTATCGG TTGCTGGCGC GGAACAAGCC CATATCCATG 
CAGATGCAGC AGGCGTTGCA GGCCGCACAG CAACAGCCAC CGCCAGGACC TCCAATTGGG 
CCACCTGGAG CACCAGGCGG ACCGCCACCA GGGAGCCAGC ATGCAGGACA ACCGCCCGTG 
CCGCCGCAAC AACAGCAACA ACCGCCACCT TCAGCAGGAA CTCCACCCCA GTGCTCCACG 
CCGCCCGCAA GCAATCCTTA TGGACCGCCA GTTCCTGGCC AGAAAATGCA GGTAGCGCCA 
CCACCGCCAC ACATGCAACA GGGACAGCCA TTGCCACCAC AACCGCCACA AGTGGGTGGA 
CCCCCGCCTA TACAACAGCA GCAACCACCT CAGCAGCAGC AACAACAGTC GCAGCCCCCG 
CCACCGGAGC CACATCAGCA CCAGTTGCCA AACGGAGGCA AACCCCTGAG TATGGGGCCA 
TCTGGCGGGC AACCACTAAT ACCTTCATCA CCCATGCAGC CACAAGTGAG AGGGACACTG 
CCAGGAATGC CGCCAGGTAG TCAGGTTCCG CAACCAGGAG GAGGTCCTCA GCGACAGGTA 
CCACCTGCGG GCATGCCGAT GCCCAAGCCC AATCGCATTA CAACGGTGGC CAAACCCGTC 
GGTCTGGATC CCATAACATT GCTGCAGGAA AGGGAGAATA GGATAGCAGC CAGGATATCA 
TTGCGCATGC AGGAGTTGCA GCGACTGCCG GCCACGATGT CCGAGGATCT GCGTCTGCAA 
GCGGCTATCG AGTTGAGGGC CCTAAGGGTC TTGAACTTCC AGCGACAGCT GCGTATGGAG 
TTTGTTCAGT GCACGAGACG AGATACCACT TTGGAAACAG CGTTAAACAT TAAGCTCTAC 
AAGAGGACCA AGAGGCAGGG CCTGCGGGAG GCACGTGCCA CCGAGAAGCT AGAGAAGCAA 
CAGAAGCTAG AGGCTGAACG AAAGCGTCGC CAAAAGCATC TGGAGTTCTT GGCTGCTGTG 
CTTCAGCACG GCAAGGATCT AAGGGAGTTC CACAGAAATA ATAAGGCCCA GCTTGCAAGG 
ATGAACAAGG CCGTGATGAA TCACCACGCC AATGCCGAGC GTGAACAGAA GAAGGAACAA 
GAGCGCATCG AAAAGGAACG TATGCGACGT CTGATGGCCG AGGATGAGGA GGGTTACCGC 
AAGCTGATTG ATCAAAAGAA GGACAAGAGA TTGGCTTTCC TACTGTCGCA GACAGATGAG 
TATATTAGCA ACCTAACTCA GATGGTGAAA CAGCACAAGG ACGACCAGAT GAAGAAAAAG 
GAGGAGGAGG GCAAGCGGCT GATACAGTTC AAAAAGGAGC TGCTGATGAG CGGAGAGTAC 
ATTGGCATTG ATGAGGGCAG CATTGTTGCC GATATGCGGG TACATGTGGT GGAACAGTGC 
ACGGGTAAGA AACTCACCGG CGATGATGCA CCCATGCTGA AGCACTTGCA CCGCTGGCTG 
AATATGCATC CTGGCTGGGA TTGGATCGAC GACGAGGAGG ACAGCTGTGG GAGCAACGAC 
GATCATAAAC CCAAGGTGGA GGAGCAACCA ACGGCAACAG AAGATGCCAC CGACAAAGCG 
CAGGCGACGG GCAACGATGA GGATGCCAAG GATCTAATTA CCAAGGCCAA GGTGGAGGAC 
GATGAGTACA GGACGGAGGA ACAGACGTAC TACAGCATCG CTCATACCAT TCATGAAAAG 
GTTGTGGAGC AGGCCAGCAT CATGGTTAAT GGTACGCTCA AGGAATACCA GATCAAAGGC 
TTGGAGTGGT TGGTTTCGCT GTACAATAAC AATCTAAATG GTATTTTGGC CGATGAAATG 
GGTTTGGGTA AAACCATTCA AACCATTTCG CTGGTAACCT ACCTTATGGA TCGAAAGAAG 
GTTATGGGTC CTTACTTGAT TATTGTACCA CTCTCCACCC TGCCCAATTG GGTGCTGGAA 
TTCGAGAAGT GGGCGCCAGC CGTGGGTGTA GTCAGCTACA AGGGCAGTCC GCAGGGAAGG 
CGATTGCTGC AAAATCAAAT GAGAGCAACC AAGTTCAATG TGCTGTTAAC CACATATGAG 
TACGTGATTA AGGACAAGGC GGTGCTGGCC AAGATCCAGT GGAAATACAT GATTATCGAC 
GAGGGTCACC GCATGAAGAA CCATCACTGC AAACTCACCC AGGTGCTGAA CACCCACTAC 
ATTGCCCCCT ATCGGTTACT TCTCACGGGT ACACCTCTGC AAAACAAGCT TCCCGAGTTG 
TGGGCGCTAC TTAATTTCCT GCTGCCCTCG ATCTTTAAGT CCTGCTCCAC ATTTGAACAG 
TGGTTCAATG CGCCATTTGC CACCACTGGC GAGAAGGTCG AGCTAAACGA GGAGGAGACC 
ATCCTTATCA TTCGTCGTCT GCACAAGGTT CTTCGTCCTT TCCTGTTGCG TCGCCTCAAA 
AAGGAGGTAG AGCACCAGCT GCCCGACAAG GTCGAGTACA TCATCAAGTG CGACATGTCC 
GCGCTGCAAC GCGTTCTCTA CAAGCACATG CAGAGCAAGG GTGTCCTGCT CACCGATGGT 
TCCGAGAAGG GCAAGCACGG CAAGGGAGGC GCTAAGGCGC TGATGAACAC GATCGTACAG 
CTGCGCAAGC TGTGCAACCA TCCGTTTATG TTCCAGCACA TAGAAGAGAA GTACTGCGAT 
CACACTGGCG GCCACGGAGT TGTATCCGGA CCGGATCTGT ATCGTGTCTC TGGCAAATTC 
GAGCTGCTTG ATCGCATCCT GCCCAAGCTG AAGGCGACCA ATCATCGCGT GCTGCTCTTC 
TGTCAGATGA CCCAATGCAT GACCATCATC GAAGACTACC TGGGATGGCG CCAATTCGGC 
TACCTTCGAC TCGACGGTAC CACGAAGGCC GAGGATCGTG GCGAACTACT GCGAAAATTC 
AACGCCAAAG GCTCGGATGT ATTTGTCTTT TTGCTATCTA CCCGAGCTGG TGGTTTGGGT 
CTTAATCTGC AGACCGCCGA TACTGTAGTA ATCTTCGACT CCGATTGGAA TCCCCATCAG 
GATCTGCAAG CCCAGGATCG TGCCCATCGT ATTGGTCAAC GTAACGAGGT GCGCGTCCTG 
CGACTAATGA CAGTCAACTC GGTGGAAGAG CGCATCTTGG CAGCAGCCAG GTACAAGCTG 
AACATGGACG AGAAGGTCAT CCAGGCTGGT ATGTTCGATC AGAAGTCAAC GGGTAGCGAG 
CGACAGCAGT TCCTACAAAC GATTCTGCAC CAGGATGACA ACGAAGAGGA GGAGGAAAAT 
GAGGTGCCCG ATGACGAAAT GATCAACATG ATGATCGCCC GCAGCGAGGA GGAGATTGAA 
ATATTTAAGC GCATGGATGC TGAGCGCAAG AAGGAGGATG AGGAGATCCA TCCGGGTAGG 
GAACGTCTGA TCGATGAGTC CGAGCTACCC GACTGGTTGA CAAAGGATGA TGACGAAGTA 
GAGCGTTTCC ACTATCAGTA CGACGAGGAT ACCATTCTAG GTCGAGGCTC CCGGCAGCGA 
AAGGAAGTAG ACTACACCGA TAGTTTGACT GAAAAGGAGT GGCTGAAAGC CATCGACGAT 
GGCGCTGAGT TCGACGAGGA AGAGGAGGAA GACGATTCGA AGCGCAAGCG ACGAAAGCGT 
AAGAATCGGA AGGAAGAGTC CGACGATGAT TCGTTGATCC TAAAACGGCG ACGACGCCAA 
AATCTGGACA AGCGGTCCAA GAAGCAGATG CACAAGATTA TGAGTGCGGT TATCAAGCAC 
AACCAAGATG GACGAACCCT GTCCGAACCG TTCATGAAGT TGCCTTCGCG GCAGAGGTTG 
CCCGACTACT ACGAGATCAT TAAGCGGCCA GTTGATATTA AGAAAATCCT GCAGCGCATT 
GAGGACTGCA AGTATGCCGA TCTCAACGAG CTGGAGAAGG ACTTTATGCA GCTATGCCAG 
AATGCACAGA TCTACAACGA AGAGGCGTCT CTGATTTACC TGGACTCGAT TGCCCTGCAA 
AAGGTATTCG TTGGGGCCAG GCAGAGGATA ACAGCTGCAG CGGATGCAGC CGCAGTGGCA 
GCCGGAGATA ACACGGGTGA AGCGCACGGC AATGGAGGTT CGGATAATTC GGACAACGAC 
GACGACGATG GTGGCGATGA CGGCTCCGAC GACGAGGAGA TTGCAACCAC TTCAGCGGCG 
GCTGTCAAAA TGAAGCTGAA ACTGAACAAA TCTCTGGCTA GTGCGCCCGC TACGCCCACG 
CAATCATCGT CGAATGTGAG CAGCGGAGCG GCTACCACAT CCAAGAAGCA GACGCGTCGC 
AAGCGCTCCC AAAAGAAGTA CACTATTTCA GACGATGACG ACGATGACAT GGACTAGCTA 
CCGTTGGGAG TGATCTTCAA GCGCGGATCG TCGGATGTCG AAGGCATGGG CAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

brm ( NM_168640.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730088.1 Drosophila melanogaster brahma (brm), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa20961
Clone ID Related Accession (Same CDS sequence)	NM_168640.2 , NM_080497.5
Accession Version	NM_168640.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	brahma, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168640.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCTCGC CCTCTCCGGC GAACAGTCCC ATGCCACCGC CACAGGCGCC CAGTCCGATG 
GCCCCGCCCT CGCAGAGTCC CGCCCCCTCG CCGCACAGCC CGTATCCGCA CCAGCAGCCG 
GGTCCACTGC AAGGACCTCC GCCACCCGGA CATCCCGGTG CCTACGGACA TCCCATGCAG 
CACGGACCAC CCGGCCAGGG TCCACCCGGG CATCATATGC CACCGCACCA CCAGGGGATG 
AGTCCGCACA TGGGAATGCA GATGCCGCCG ACGGGACCGA ACATGTCCCC CTACCAAACC 
CACGGAATGC CACCCAATGC ACCCACACAA CCCTGCATAG TGTCACCTGG TGGCCCACCT 
GGAGGCCCGC CACCTCCGGA GCGCTCGAGC CAGGAGAACC TGCACGCGCT TCAACGCGCC 
ATTGATTCAA TGGAGGAAAA GGGCTTGCAA GAGGATCCTC GCTACTCGCA GCTGCTGGCA 
ATGCGAGCAA CCTCGAAGCA TCAGCACCTT AATGGCAATC AGGTTAACTT GTTGCGCACA 
CAGATCACCG CCTATCGGTT GCTGGCGCGG AACAAGCCCA TATCCATGCA GATGCAGCAG 
GCGTTGCAGG CCGCACAGCA ACAGCCACCG CCAGGACCTC CAATTGGGCC ACCTGGAGCA 
CCAGGCGGAC CGCCACCAGG GAGCCAGCAT GCAGGACAAC CGCCCGTGCC GCCGCAACAA 
CAGCAACAAC CGCCACCTTC AGCAGGAACT CCACCCCAGT GCTCCACGCC GCCCGCAAGC 
AATCCTTATG GACCGCCAGT TCCTGGCCAG AAAATGCAGG TAGCGCCACC ACCGCCACAC 
ATGCAACAGG GACAGCCATT GCCACCACAA CCGCCACAAG TGGGTGGACC CCCGCCTATA 
CAACAGCAGC AACCACCTCA GCAGCAGCAA CAACAGTCGC AGCCCCCGCC ACCGGAGCCA 
CATCAGCACC AGTTGCCAAA CGGAGGCAAA CCCCTGAGTA TGGGGCCATC TGGCGGGCAA 
CCACTAATAC CTTCATCACC CATGCAGCCA CAAGTGAGAG GGACACTGCC AGGAATGCCG 
CCAGGTAGTC AGGTTCCGCA ACCAGGAGGA GGTCCTCAGC GACAGGTACC ACCTGCGGGC 
ATGCCGATGC CCAAGCCCAA TCGCATTACA ACGGTGGCCA AACCCGTCGG TCTGGATCCC 
ATAACATTGC TGCAGGAAAG GGAGAATAGG ATAGCAGCCA GGATATCATT GCGCATGCAG 
GAGTTGCAGC GACTGCCGGC CACGATGTCC GAGGATCTGC GTCTGCAAGC GGCTATCGAG 
TTGAGGGCCC TAAGGGTCTT GAACTTCCAG CGACAGCTGC GTATGGAGTT TGTTCAGTGC 
ACGAGACGAG ATACCACTTT GGAAACAGCG TTAAACATTA AGCTCTACAA GAGGACCAAG 
AGGCAGGGCC TGCGGGAGGC ACGTGCCACC GAGAAGCTAG AGAAGCAACA GAAGCTAGAG 
GCTGAACGAA AGCGTCGCCA AAAGCATCTG GAGTTCTTGG CTGCTGTGCT TCAGCACGGC 
AAGGATCTAA GGGAGTTCCA CAGAAATAAT AAGGCCCAGC TTGCAAGGAT GAACAAGGCC 
GTGATGAATC ACCACGCCAA TGCCGAGCGT GAACAGAAGA AGGAACAAGA GCGCATCGAA 
AAGGAACGTA TGCGACGTCT GATGGCCGAG GATGAGGAGG GTTACCGCAA GCTGATTGAT 
CAAAAGAAGG ACAAGAGATT GGCTTTCCTA CTGTCGCAGA CAGATGAGTA TATTAGCAAC 
CTAACTCAGA TGGTGAAACA GCACAAGGAC GACCAGATGA AGAAAAAGGA GGAGGAGGGC 
AAGCGGCTGA TACAGTTCAA AAAGGAGCTG CTGATGAGCG GAGAGTACAT TGGCATTGAT 
GAGGGCAGCA TTGTTGCCGA TATGCGGGTA CATGTGGTGG AACAGTGCAC GGGTAAGAAA 
CTCACCGGCG ATGATGCACC CATGCTGAAG CACTTGCACC GCTGGCTGAA TATGCATCCT 
GGCTGGGATT GGATCGACGA CGAGGAGGAC AGCTGTGGGA GCAACGACGA TCATAAACCC 
AAGGTGGAGG AGCAACCAAC GGCAACAGAA GATGCCACCG ACAAAGCGCA GGCGACGGGC 
AACGATGAGG ATGCCAAGGA TCTAATTACC AAGGCCAAGG TGGAGGACGA TGAGTACAGG 
ACGGAGGAAC AGACGTACTA CAGCATCGCT CATACCATTC ATGAAAAGGT TGTGGAGCAG 
GCCAGCATCA TGGTTAATGG TACGCTCAAG GAATACCAGA TCAAAGGCTT GGAGTGGTTG 
GTTTCGCTGT ACAATAACAA TCTAAATGGT ATTTTGGCCG ATGAAATGGG TTTGGGTAAA 
ACCATTCAAA CCATTTCGCT GGTAACCTAC CTTATGGATC GAAAGAAGGT TATGGGTCCT 
TACTTGATTA TTGTACCACT CTCCACCCTG CCCAATTGGG TGCTGGAATT CGAGAAGTGG 
GCGCCAGCCG TGGGTGTAGT CAGCTACAAG GGCAGTCCGC AGGGAAGGCG ATTGCTGCAA 
AATCAAATGA GAGCAACCAA GTTCAATGTG CTGTTAACCA CATATGAGTA CGTGATTAAG 
GACAAGGCGG TGCTGGCCAA GATCCAGTGG AAATACATGA TTATCGACGA GGGTCACCGC 
ATGAAGAACC ATCACTGCAA ACTCACCCAG GTGCTGAACA CCCACTACAT TGCCCCCTAT 
CGGTTACTTC TCACGGGTAC ACCTCTGCAA AACAAGCTTC CCGAGTTGTG GGCGCTACTT 
AATTTCCTGC TGCCCTCGAT CTTTAAGTCC TGCTCCACAT TTGAACAGTG GTTCAATGCG 
CCATTTGCCA CCACTGGCGA GAAGGTCGAG CTAAACGAGG AGGAGACCAT CCTTATCATT 
CGTCGTCTGC ACAAGGTTCT TCGTCCTTTC CTGTTGCGTC GCCTCAAAAA GGAGGTAGAG 
CACCAGCTGC CCGACAAGGT CGAGTACATC ATCAAGTGCG ACATGTCCGC GCTGCAACGC 
GTTCTCTACA AGCACATGCA GAGCAAGGGT GTCCTGCTCA CCGATGGTTC CGAGAAGGGC 
AAGCACGGCA AGGGAGGCGC TAAGGCGCTG ATGAACACGA TCGTACAGCT GCGCAAGCTG 
TGCAACCATC CGTTTATGTT CCAGCACATA GAAGAGAAGT ACTGCGATCA CACTGGCGGC 
CACGGAGTTG TATCCGGACC GGATCTGTAT CGTGTCTCTG GCAAATTCGA GCTGCTTGAT 
CGCATCCTGC CCAAGCTGAA GGCGACCAAT CATCGCGTGC TGCTCTTCTG TCAGATGACC 
CAATGCATGA CCATCATCGA AGACTACCTG GGATGGCGCC AATTCGGCTA CCTTCGACTC 
GACGGTACCA CGAAGGCCGA GGATCGTGGC GAACTACTGC GAAAATTCAA CGCCAAAGGC 
TCGGATGTAT TTGTCTTTTT GCTATCTACC CGAGCTGGTG GTTTGGGTCT TAATCTGCAG 
ACCGCCGATA CTGTAGTAAT CTTCGACTCC GATTGGAATC CCCATCAGGA TCTGCAAGCC 
CAGGATCGTG CCCATCGTAT TGGTCAACGT AACGAGGTGC GCGTCCTGCG ACTAATGACA 
GTCAACTCGG TGGAAGAGCG CATCTTGGCA GCAGCCAGGT ACAAGCTGAA CATGGACGAG 
AAGGTCATCC AGGCTGGTAT GTTCGATCAG AAGTCAACGG GTAGCGAGCG ACAGCAGTTC 
CTACAAACGA TTCTGCACCA GGATGACAAC GAAGAGGAGG AGGAAAATGA GGTGCCCGAT 
GACGAAATGA TCAACATGAT GATCGCCCGC AGCGAGGAGG AGATTGAAAT ATTTAAGCGC 
ATGGATGCTG AGCGCAAGAA GGAGGATGAG GAGATCCATC CGGGTAGGGA ACGTCTGATC 
GATGAGTCCG AGCTACCCGA CTGGTTGACA AAGGATGATG ACGAAGTAGA GCGTTTCCAC 
TATCAGTACG ACGAGGATAC CATTCTAGGT CGAGGCTCCC GGCAGCGAAA GGAAGTAGAC 
TACACCGATA GTTTGACTGA AAAGGAGTGG CTGAAAGCCA TCGACGATGG CGCTGAGTTC 
GACGAGGAAG AGGAGGAAGA CGATTCGAAG CGCAAGCGAC GAAAGCGTAA GAATCGGAAG 
GAAGAGTCCG ACGATGATTC GTTGATCCTA AAACGGCGAC GACGCCAAAA TCTGGACAAG 
CGGTCCAAGA AGCAGATGCA CAAGATTATG AGTGCGGTTA TCAAGCACAA CCAAGATGGA 
CGAACCCTGT CCGAACCGTT CATGAAGTTG CCTTCGCGGC AGAGGTTGCC CGACTACTAC 
GAGATCATTA AGCGGCCAGT TGATATTAAG AAAATCCTGC AGCGCATTGA GGACTGCAAG 
TATGCCGATC TCAACGAGCT GGAGAAGGAC TTTATGCAGC TATGCCAGAA TGCACAGATC 
TACAACGAAG AGGCGTCTCT GATTTACCTG GACTCGATTG CCCTGCAAAA GGTATTCGTT 
GGGGCCAGGC AGAGGATAAC AGCTGCAGCG GATGCAGCCG CAGTGGCAGC CGGAGATAAC 
ACGGGTGAAG CGCACGGCAA TGGAGGTTCG GATAATTCGG ACAACGACGA CGACGATGGT 
GGCGATGACG GCTCCGACGA CGAGGAGATT GCAACCACTT CAGCGGCGGC TGTCAAAATG 
AAGCTGAAAC TGAACAAATC TCTGGCTAGT GCGCCCGCTA CGCCCACGCA ATCATCGTCG 
AATGTGAGCA GCGGAGCGGC TACCACATCC AAGAAGCAGA CGCGTCGCAA GCGCTCCCAA 
AAGAAGTACA CTATTTCAGA CGATGACGAC GATGACATGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730088.1
CDS	123..5027
Translation	MASPSPANSPMPPPQAPSPMAPPSQSPAPSPHSPYPHQQPGPLQGPPPPGHPGAYGHPMQHGPPGQGPPGHHMPPHHQGMSPHMGMQMPPTGPNMSPYQTHGMPPNAPTQPCIVSPGGPPGGPPPPERSSQENLHALQRAIDSMEEKGLQEDPRYSQLLAMRATSKHQHLNGNQVNLLRTQITAYRLLARNKPISMQMQQALQAAQQQPPPGPPIGPPGAPGGPPPGSQHAGQPPVPPQQQQQPPPSAGTPPQCSTPPASNPYGPPVPGQKMQVAPPPPHMQQGQPLPPQPPQVGGPPPIQQQQPPQQQQQQSQPPPPEPHQHQLPNGGKPLSMGPSGGQPLIPSSPMQPQVRGTLPGMPPGSQVPQPGGGPQRQVPPAGMPMPKPNRITTVAKPVGLDPITLLQERENRIAARISLRMQELQRLPATMSEDLRLQAAIELRALRVLNFQRQLRMEFVQCTRRDTTLETALNIKLYKRTKRQGLREARATEKLEKQQKLEAERKRRQKHLEFLAAVLQHGKDLREFHRNNKAQLARMNKAVMNHHANAEREQKKEQERIEKERMRRLMAEDEEGYRKLIDQKKDKRLAFLLSQTDEYISNLTQMVKQHKDDQMKKKEEEGKRLIQFKKELLMSGEYIGIDEGSIVADMRVHVVEQCTGKKLTGDDAPMLKHLHRWLNMHPGWDWIDDEEDSCGSNDDHKPKVEEQPTATEDATDKAQATGNDEDAKDLITKAKVEDDEYRTEEQTYYSIAHTIHEKVVEQASIMVNGTLKEYQIKGLEWLVSLYNNNLNGILADEMGLGKTIQTISLVTYLMDRKKVMGPYLIIVPLSTLPNWVLEFEKWAPAVGVVSYKGSPQGRRLLQNQMRATKFNVLLTTYEYVIKDKAVLAKIQWKYMIIDEGHRMKNHHCKLTQVLNTHYIAPYRLLLTGTPLQNKLPELWALLNFLLPSIFKSCSTFEQWFNAPFATTGEKVELNEEETILIIRRLHKVLRPFLLRRLKKEVEHQLPDKVEYIIKCDMSALQRVLYKHMQSKGVLLTDGSEKGKHGKGGAKALMNTIVQLRKLCNHPFMFQHIEEKYCDHTGGHGVVSGPDLYRVSGKFELLDRILPKLKATNHRVLLFCQMTQCMTIIEDYLGWRQFGYLRLDGTTKAEDRGELLRKFNAKGSDVFVFLLSTRAGGLGLNLQTADTVVIFDSDWNPHQDLQAQDRAHRIGQRNEVRVLRLMTVNSVEERILAAARYKLNMDEKVIQAGMFDQKSTGSERQQFLQTILHQDDNEEEEENEVPDDEMINMMIARSEEEIEIFKRMDAERKKEDEEIHPGRERLIDESELPDWLTKDDDEVERFHYQYDEDTILGRGSRQRKEVDYTDSLTEKEWLKAIDDGAEFDEEEEEDDSKRKRRKRKNRKEESDDDSLILKRRRRQNLDKRSKKQMHKIMSAVIKHNQDGRTLSEPFMKLPSRQRLPDYYEIIKRPVDIKKILQRIEDCKYADLNELEKDFMQLCQNAQIYNEEASLIYLDSIALQKVFVGARQRITAAADAAAVAAGDNTGEAHGNGGSDNSDNDDDDGGDDGSDDEEIATTSAAAVKMKLKLNKSLASAPATPTQSSSNVSSGAATTSKKQTRRKRSQKKYTISDDDDDDMD

Target ORF information:

RefSeq Version	NM_168640.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster brahma (brm), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168640.2

ORF Insert Sequence:

ATGGCCTCGC CCTCTCCGGC GAACAGTCCC ATGCCACCGC CACAGGCGCC CAGTCCGATG 
GCCCCGCCCT CGCAGAGTCC CGCCCCCTCG CCGCACAGCC CGTATCCGCA CCAGCAGCCG 
GGTCCACTGC AAGGACCTCC GCCACCCGGA CATCCCGGTG CCTACGGACA TCCCATGCAG 
CACGGACCAC CCGGCCAGGG TCCACCCGGG CATCATATGC CACCGCACCA CCAGGGGATG 
AGTCCGCACA TGGGAATGCA GATGCCGCCG ACGGGACCGA ACATGTCCCC CTACCAAACC 
CACGGAATGC CACCCAATGC ACCCACACAA CCCTGCATAG TGTCACCTGG TGGCCCACCT 
GGAGGCCCGC CACCTCCGGA GCGCTCGAGC CAGGAGAACC TGCACGCGCT TCAACGCGCC 
ATTGATTCAA TGGAGGAAAA GGGCTTGCAA GAGGATCCTC GCTACTCGCA GCTGCTGGCA 
ATGCGAGCAA CCTCGAAGCA TCAGCACCTT AATGGCAATC AGGTTAACTT GTTGCGCACA 
CAGATCACCG CCTATCGGTT GCTGGCGCGG AACAAGCCCA TATCCATGCA GATGCAGCAG 
GCGTTGCAGG CCGCACAGCA ACAGCCACCG CCAGGACCTC CAATTGGGCC ACCTGGAGCA 
CCAGGCGGAC CGCCACCAGG GAGCCAGCAT GCAGGACAAC CGCCCGTGCC GCCGCAACAA 
CAGCAACAAC CGCCACCTTC AGCAGGAACT CCACCCCAGT GCTCCACGCC GCCCGCAAGC 
AATCCTTATG GACCGCCAGT TCCTGGCCAG AAAATGCAGG TAGCGCCACC ACCGCCACAC 
ATGCAACAGG GACAGCCATT GCCACCACAA CCGCCACAAG TGGGTGGACC CCCGCCTATA 
CAACAGCAGC AACCACCTCA GCAGCAGCAA CAACAGTCGC AGCCCCCGCC ACCGGAGCCA 
CATCAGCACC AGTTGCCAAA CGGAGGCAAA CCCCTGAGTA TGGGGCCATC TGGCGGGCAA 
CCACTAATAC CTTCATCACC CATGCAGCCA CAAGTGAGAG GGACACTGCC AGGAATGCCG 
CCAGGTAGTC AGGTTCCGCA ACCAGGAGGA GGTCCTCAGC GACAGGTACC ACCTGCGGGC 
ATGCCGATGC CCAAGCCCAA TCGCATTACA ACGGTGGCCA AACCCGTCGG TCTGGATCCC 
ATAACATTGC TGCAGGAAAG GGAGAATAGG ATAGCAGCCA GGATATCATT GCGCATGCAG 
GAGTTGCAGC GACTGCCGGC CACGATGTCC GAGGATCTGC GTCTGCAAGC GGCTATCGAG 
TTGAGGGCCC TAAGGGTCTT GAACTTCCAG CGACAGCTGC GTATGGAGTT TGTTCAGTGC 
ACGAGACGAG ATACCACTTT GGAAACAGCG TTAAACATTA AGCTCTACAA GAGGACCAAG 
AGGCAGGGCC TGCGGGAGGC ACGTGCCACC GAGAAGCTAG AGAAGCAACA GAAGCTAGAG 
GCTGAACGAA AGCGTCGCCA AAAGCATCTG GAGTTCTTGG CTGCTGTGCT TCAGCACGGC 
AAGGATCTAA GGGAGTTCCA CAGAAATAAT AAGGCCCAGC TTGCAAGGAT GAACAAGGCC 
GTGATGAATC ACCACGCCAA TGCCGAGCGT GAACAGAAGA AGGAACAAGA GCGCATCGAA 
AAGGAACGTA TGCGACGTCT GATGGCCGAG GATGAGGAGG GTTACCGCAA GCTGATTGAT 
CAAAAGAAGG ACAAGAGATT GGCTTTCCTA CTGTCGCAGA CAGATGAGTA TATTAGCAAC 
CTAACTCAGA TGGTGAAACA GCACAAGGAC GACCAGATGA AGAAAAAGGA GGAGGAGGGC 
AAGCGGCTGA TACAGTTCAA AAAGGAGCTG CTGATGAGCG GAGAGTACAT TGGCATTGAT 
GAGGGCAGCA TTGTTGCCGA TATGCGGGTA CATGTGGTGG AACAGTGCAC GGGTAAGAAA 
CTCACCGGCG ATGATGCACC CATGCTGAAG CACTTGCACC GCTGGCTGAA TATGCATCCT 
GGCTGGGATT GGATCGACGA CGAGGAGGAC AGCTGTGGGA GCAACGACGA TCATAAACCC 
AAGGTGGAGG AGCAACCAAC GGCAACAGAA GATGCCACCG ACAAAGCGCA GGCGACGGGC 
AACGATGAGG ATGCCAAGGA TCTAATTACC AAGGCCAAGG TGGAGGACGA TGAGTACAGG 
ACGGAGGAAC AGACGTACTA CAGCATCGCT CATACCATTC ATGAAAAGGT TGTGGAGCAG 
GCCAGCATCA TGGTTAATGG TACGCTCAAG GAATACCAGA TCAAAGGCTT GGAGTGGTTG 
GTTTCGCTGT ACAATAACAA TCTAAATGGT ATTTTGGCCG ATGAAATGGG TTTGGGTAAA 
ACCATTCAAA CCATTTCGCT GGTAACCTAC CTTATGGATC GAAAGAAGGT TATGGGTCCT 
TACTTGATTA TTGTACCACT CTCCACCCTG CCCAATTGGG TGCTGGAATT CGAGAAGTGG 
GCGCCAGCCG TGGGTGTAGT CAGCTACAAG GGCAGTCCGC AGGGAAGGCG ATTGCTGCAA 
AATCAAATGA GAGCAACCAA GTTCAATGTG CTGTTAACCA CATATGAGTA CGTGATTAAG 
GACAAGGCGG TGCTGGCCAA GATCCAGTGG AAATACATGA TTATCGACGA GGGTCACCGC 
ATGAAGAACC ATCACTGCAA ACTCACCCAG GTGCTGAACA CCCACTACAT TGCCCCCTAT 
CGGTTACTTC TCACGGGTAC ACCTCTGCAA AACAAGCTTC CCGAGTTGTG GGCGCTACTT 
AATTTCCTGC TGCCCTCGAT CTTTAAGTCC TGCTCCACAT TTGAACAGTG GTTCAATGCG 
CCATTTGCCA CCACTGGCGA GAAGGTCGAG CTAAACGAGG AGGAGACCAT CCTTATCATT 
CGTCGTCTGC ACAAGGTTCT TCGTCCTTTC CTGTTGCGTC GCCTCAAAAA GGAGGTAGAG 
CACCAGCTGC CCGACAAGGT CGAGTACATC ATCAAGTGCG ACATGTCCGC GCTGCAACGC 
GTTCTCTACA AGCACATGCA GAGCAAGGGT GTCCTGCTCA CCGATGGTTC CGAGAAGGGC 
AAGCACGGCA AGGGAGGCGC TAAGGCGCTG ATGAACACGA TCGTACAGCT GCGCAAGCTG 
TGCAACCATC CGTTTATGTT CCAGCACATA GAAGAGAAGT ACTGCGATCA CACTGGCGGC 
CACGGAGTTG TATCCGGACC GGATCTGTAT CGTGTCTCTG GCAAATTCGA GCTGCTTGAT 
CGCATCCTGC CCAAGCTGAA GGCGACCAAT CATCGCGTGC TGCTCTTCTG TCAGATGACC 
CAATGCATGA CCATCATCGA AGACTACCTG GGATGGCGCC AATTCGGCTA CCTTCGACTC 
GACGGTACCA CGAAGGCCGA GGATCGTGGC GAACTACTGC GAAAATTCAA CGCCAAAGGC 
TCGGATGTAT TTGTCTTTTT GCTATCTACC CGAGCTGGTG GTTTGGGTCT TAATCTGCAG 
ACCGCCGATA CTGTAGTAAT CTTCGACTCC GATTGGAATC CCCATCAGGA TCTGCAAGCC 
CAGGATCGTG CCCATCGTAT TGGTCAACGT AACGAGGTGC GCGTCCTGCG ACTAATGACA 
GTCAACTCGG TGGAAGAGCG CATCTTGGCA GCAGCCAGGT ACAAGCTGAA CATGGACGAG 
AAGGTCATCC AGGCTGGTAT GTTCGATCAG AAGTCAACGG GTAGCGAGCG ACAGCAGTTC 
CTACAAACGA TTCTGCACCA GGATGACAAC GAAGAGGAGG AGGAAAATGA GGTGCCCGAT 
GACGAAATGA TCAACATGAT GATCGCCCGC AGCGAGGAGG AGATTGAAAT ATTTAAGCGC 
ATGGATGCTG AGCGCAAGAA GGAGGATGAG GAGATCCATC CGGGTAGGGA ACGTCTGATC 
GATGAGTCCG AGCTACCCGA CTGGTTGACA AAGGATGATG ACGAAGTAGA GCGTTTCCAC 
TATCAGTACG ACGAGGATAC CATTCTAGGT CGAGGCTCCC GGCAGCGAAA GGAAGTAGAC 
TACACCGATA GTTTGACTGA AAAGGAGTGG CTGAAAGCCA TCGACGATGG CGCTGAGTTC 
GACGAGGAAG AGGAGGAAGA CGATTCGAAG CGCAAGCGAC GAAAGCGTAA GAATCGGAAG 
GAAGAGTCCG ACGATGATTC GTTGATCCTA AAACGGCGAC GACGCCAAAA TCTGGACAAG 
CGGTCCAAGA AGCAGATGCA CAAGATTATG AGTGCGGTTA TCAAGCACAA CCAAGATGGA 
CGAACCCTGT CCGAACCGTT CATGAAGTTG CCTTCGCGGC AGAGGTTGCC CGACTACTAC 
GAGATCATTA AGCGGCCAGT TGATATTAAG AAAATCCTGC AGCGCATTGA GGACTGCAAG 
TATGCCGATC TCAACGAGCT GGAGAAGGAC TTTATGCAGC TATGCCAGAA TGCACAGATC 
TACAACGAAG AGGCGTCTCT GATTTACCTG GACTCGATTG CCCTGCAAAA GGTATTCGTT 
GGGGCCAGGC AGAGGATAAC AGCTGCAGCG GATGCAGCCG CAGTGGCAGC CGGAGATAAC 
ACGGGTGAAG CGCACGGCAA TGGAGGTTCG GATAATTCGG ACAACGACGA CGACGATGGT 
GGCGATGACG GCTCCGACGA CGAGGAGATT GCAACCACTT CAGCGGCGGC TGTCAAAATG 
AAGCTGAAAC TGAACAAATC TCTGGCTAGT GCGCCCGCTA CGCCCACGCA ATCATCGTCG 
AATGTGAGCA GCGGAGCGGC TACCACATCC AAGAAGCAGA CGCGTCGCAA GCGCTCCCAA 
AAGAAGTACA CTATTTCAGA CGATGACGAC GATGACATGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

brm ( NM_080497.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_536745.4 Drosophila melanogaster brahma (brm), transcript variant C, mRNA.

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CloneID	OFa20961
Clone ID Related Accession (Same CDS sequence)	NM_168640.2 , NM_080497.5
Accession Version	NM_080497.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	brahma, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_080497.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCTCGC CCTCTCCGGC GAACAGTCCC ATGCCACCGC CACAGGCGCC CAGTCCGATG 
GCCCCGCCCT CGCAGAGTCC CGCCCCCTCG CCGCACAGCC CGTATCCGCA CCAGCAGCCG 
GGTCCACTGC AAGGACCTCC GCCACCCGGA CATCCCGGTG CCTACGGACA TCCCATGCAG 
CACGGACCAC CCGGCCAGGG TCCACCCGGG CATCATATGC CACCGCACCA CCAGGGGATG 
AGTCCGCACA TGGGAATGCA GATGCCGCCG ACGGGACCGA ACATGTCCCC CTACCAAACC 
CACGGAATGC CACCCAATGC ACCCACACAA CCCTGCATAG TGTCACCTGG TGGCCCACCT 
GGAGGCCCGC CACCTCCGGA GCGCTCGAGC CAGGAGAACC TGCACGCGCT TCAACGCGCC 
ATTGATTCAA TGGAGGAAAA GGGCTTGCAA GAGGATCCTC GCTACTCGCA GCTGCTGGCA 
ATGCGAGCAA CCTCGAAGCA TCAGCACCTT AATGGCAATC AGGTTAACTT GTTGCGCACA 
CAGATCACCG CCTATCGGTT GCTGGCGCGG AACAAGCCCA TATCCATGCA GATGCAGCAG 
GCGTTGCAGG CCGCACAGCA ACAGCCACCG CCAGGACCTC CAATTGGGCC ACCTGGAGCA 
CCAGGCGGAC CGCCACCAGG GAGCCAGCAT GCAGGACAAC CGCCCGTGCC GCCGCAACAA 
CAGCAACAAC CGCCACCTTC AGCAGGAACT CCACCCCAGT GCTCCACGCC GCCCGCAAGC 
AATCCTTATG GACCGCCAGT TCCTGGCCAG AAAATGCAGG TAGCGCCACC ACCGCCACAC 
ATGCAACAGG GACAGCCATT GCCACCACAA CCGCCACAAG TGGGTGGACC CCCGCCTATA 
CAACAGCAGC AACCACCTCA GCAGCAGCAA CAACAGTCGC AGCCCCCGCC ACCGGAGCCA 
CATCAGCACC AGTTGCCAAA CGGAGGCAAA CCCCTGAGTA TGGGGCCATC TGGCGGGCAA 
CCACTAATAC CTTCATCACC CATGCAGCCA CAAGTGAGAG GGACACTGCC AGGAATGCCG 
CCAGGTAGTC AGGTTCCGCA ACCAGGAGGA GGTCCTCAGC GACAGGTACC ACCTGCGGGC 
ATGCCGATGC CCAAGCCCAA TCGCATTACA ACGGTGGCCA AACCCGTCGG TCTGGATCCC 
ATAACATTGC TGCAGGAAAG GGAGAATAGG ATAGCAGCCA GGATATCATT GCGCATGCAG 
GAGTTGCAGC GACTGCCGGC CACGATGTCC GAGGATCTGC GTCTGCAAGC GGCTATCGAG 
TTGAGGGCCC TAAGGGTCTT GAACTTCCAG CGACAGCTGC GTATGGAGTT TGTTCAGTGC 
ACGAGACGAG ATACCACTTT GGAAACAGCG TTAAACATTA AGCTCTACAA GAGGACCAAG 
AGGCAGGGCC TGCGGGAGGC ACGTGCCACC GAGAAGCTAG AGAAGCAACA GAAGCTAGAG 
GCTGAACGAA AGCGTCGCCA AAAGCATCTG GAGTTCTTGG CTGCTGTGCT TCAGCACGGC 
AAGGATCTAA GGGAGTTCCA CAGAAATAAT AAGGCCCAGC TTGCAAGGAT GAACAAGGCC 
GTGATGAATC ACCACGCCAA TGCCGAGCGT GAACAGAAGA AGGAACAAGA GCGCATCGAA 
AAGGAACGTA TGCGACGTCT GATGGCCGAG GATGAGGAGG GTTACCGCAA GCTGATTGAT 
CAAAAGAAGG ACAAGAGATT GGCTTTCCTA CTGTCGCAGA CAGATGAGTA TATTAGCAAC 
CTAACTCAGA TGGTGAAACA GCACAAGGAC GACCAGATGA AGAAAAAGGA GGAGGAGGGC 
AAGCGGCTGA TACAGTTCAA AAAGGAGCTG CTGATGAGCG GAGAGTACAT TGGCATTGAT 
GAGGGCAGCA TTGTTGCCGA TATGCGGGTA CATGTGGTGG AACAGTGCAC GGGTAAGAAA 
CTCACCGGCG ATGATGCACC CATGCTGAAG CACTTGCACC GCTGGCTGAA TATGCATCCT 
GGCTGGGATT GGATCGACGA CGAGGAGGAC AGCTGTGGGA GCAACGACGA TCATAAACCC 
AAGGTGGAGG AGCAACCAAC GGCAACAGAA GATGCCACCG ACAAAGCGCA GGCGACGGGC 
AACGATGAGG ATGCCAAGGA TCTAATTACC AAGGCCAAGG TGGAGGACGA TGAGTACAGG 
ACGGAGGAAC AGACGTACTA CAGCATCGCT CATACCATTC ATGAAAAGGT TGTGGAGCAG 
GCCAGCATCA TGGTTAATGG TACGCTCAAG GAATACCAGA TCAAAGGCTT GGAGTGGTTG 
GTTTCGCTGT ACAATAACAA TCTAAATGGT ATTTTGGCCG ATGAAATGGG TTTGGGTAAA 
ACCATTCAAA CCATTTCGCT GGTAACCTAC CTTATGGATC GAAAGAAGGT TATGGGTCCT 
TACTTGATTA TTGTACCACT CTCCACCCTG CCCAATTGGG TGCTGGAATT CGAGAAGTGG 
GCGCCAGCCG TGGGTGTAGT CAGCTACAAG GGCAGTCCGC AGGGAAGGCG ATTGCTGCAA 
AATCAAATGA GAGCAACCAA GTTCAATGTG CTGTTAACCA CATATGAGTA CGTGATTAAG 
GACAAGGCGG TGCTGGCCAA GATCCAGTGG AAATACATGA TTATCGACGA GGGTCACCGC 
ATGAAGAACC ATCACTGCAA ACTCACCCAG GTGCTGAACA CCCACTACAT TGCCCCCTAT 
CGGTTACTTC TCACGGGTAC ACCTCTGCAA AACAAGCTTC CCGAGTTGTG GGCGCTACTT 
AATTTCCTGC TGCCCTCGAT CTTTAAGTCC TGCTCCACAT TTGAACAGTG GTTCAATGCG 
CCATTTGCCA CCACTGGCGA GAAGGTCGAG CTAAACGAGG AGGAGACCAT CCTTATCATT 
CGTCGTCTGC ACAAGGTTCT TCGTCCTTTC CTGTTGCGTC GCCTCAAAAA GGAGGTAGAG 
CACCAGCTGC CCGACAAGGT CGAGTACATC ATCAAGTGCG ACATGTCCGC GCTGCAACGC 
GTTCTCTACA AGCACATGCA GAGCAAGGGT GTCCTGCTCA CCGATGGTTC CGAGAAGGGC 
AAGCACGGCA AGGGAGGCGC TAAGGCGCTG ATGAACACGA TCGTACAGCT GCGCAAGCTG 
TGCAACCATC CGTTTATGTT CCAGCACATA GAAGAGAAGT ACTGCGATCA CACTGGCGGC 
CACGGAGTTG TATCCGGACC GGATCTGTAT CGTGTCTCTG GCAAATTCGA GCTGCTTGAT 
CGCATCCTGC CCAAGCTGAA GGCGACCAAT CATCGCGTGC TGCTCTTCTG TCAGATGACC 
CAATGCATGA CCATCATCGA AGACTACCTG GGATGGCGCC AATTCGGCTA CCTTCGACTC 
GACGGTACCA CGAAGGCCGA GGATCGTGGC GAACTACTGC GAAAATTCAA CGCCAAAGGC 
TCGGATGTAT TTGTCTTTTT GCTATCTACC CGAGCTGGTG GTTTGGGTCT TAATCTGCAG 
ACCGCCGATA CTGTAGTAAT CTTCGACTCC GATTGGAATC CCCATCAGGA TCTGCAAGCC 
CAGGATCGTG CCCATCGTAT TGGTCAACGT AACGAGGTGC GCGTCCTGCG ACTAATGACA 
GTCAACTCGG TGGAAGAGCG CATCTTGGCA GCAGCCAGGT ACAAGCTGAA CATGGACGAG 
AAGGTCATCC AGGCTGGTAT GTTCGATCAG AAGTCAACGG GTAGCGAGCG ACAGCAGTTC 
CTACAAACGA TTCTGCACCA GGATGACAAC GAAGAGGAGG AGGAAAATGA GGTGCCCGAT 
GACGAAATGA TCAACATGAT GATCGCCCGC AGCGAGGAGG AGATTGAAAT ATTTAAGCGC 
ATGGATGCTG AGCGCAAGAA GGAGGATGAG GAGATCCATC CGGGTAGGGA ACGTCTGATC 
GATGAGTCCG AGCTACCCGA CTGGTTGACA AAGGATGATG ACGAAGTAGA GCGTTTCCAC 
TATCAGTACG ACGAGGATAC CATTCTAGGT CGAGGCTCCC GGCAGCGAAA GGAAGTAGAC 
TACACCGATA GTTTGACTGA AAAGGAGTGG CTGAAAGCCA TCGACGATGG CGCTGAGTTC 
GACGAGGAAG AGGAGGAAGA CGATTCGAAG CGCAAGCGAC GAAAGCGTAA GAATCGGAAG 
GAAGAGTCCG ACGATGATTC GTTGATCCTA AAACGGCGAC GACGCCAAAA TCTGGACAAG 
CGGTCCAAGA AGCAGATGCA CAAGATTATG AGTGCGGTTA TCAAGCACAA CCAAGATGGA 
CGAACCCTGT CCGAACCGTT CATGAAGTTG CCTTCGCGGC AGAGGTTGCC CGACTACTAC 
GAGATCATTA AGCGGCCAGT TGATATTAAG AAAATCCTGC AGCGCATTGA GGACTGCAAG 
TATGCCGATC TCAACGAGCT GGAGAAGGAC TTTATGCAGC TATGCCAGAA TGCACAGATC 
TACAACGAAG AGGCGTCTCT GATTTACCTG GACTCGATTG CCCTGCAAAA GGTATTCGTT 
GGGGCCAGGC AGAGGATAAC AGCTGCAGCG GATGCAGCCG CAGTGGCAGC CGGAGATAAC 
ACGGGTGAAG CGCACGGCAA TGGAGGTTCG GATAATTCGG ACAACGACGA CGACGATGGT 
GGCGATGACG GCTCCGACGA CGAGGAGATT GCAACCACTT CAGCGGCGGC TGTCAAAATG 
AAGCTGAAAC TGAACAAATC TCTGGCTAGT GCGCCCGCTA CGCCCACGCA ATCATCGTCG 
AATGTGAGCA GCGGAGCGGC TACCACATCC AAGAAGCAGA CGCGTCGCAA GCGCTCCCAA 
AAGAAGTACA CTATTTCAGA CGATGACGAC GATGACATGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_536745.4
CDS	128..5032
Translation	MASPSPANSPMPPPQAPSPMAPPSQSPAPSPHSPYPHQQPGPLQGPPPPGHPGAYGHPMQHGPPGQGPPGHHMPPHHQGMSPHMGMQMPPTGPNMSPYQTHGMPPNAPTQPCIVSPGGPPGGPPPPERSSQENLHALQRAIDSMEEKGLQEDPRYSQLLAMRATSKHQHLNGNQVNLLRTQITAYRLLARNKPISMQMQQALQAAQQQPPPGPPIGPPGAPGGPPPGSQHAGQPPVPPQQQQQPPPSAGTPPQCSTPPASNPYGPPVPGQKMQVAPPPPHMQQGQPLPPQPPQVGGPPPIQQQQPPQQQQQQSQPPPPEPHQHQLPNGGKPLSMGPSGGQPLIPSSPMQPQVRGTLPGMPPGSQVPQPGGGPQRQVPPAGMPMPKPNRITTVAKPVGLDPITLLQERENRIAARISLRMQELQRLPATMSEDLRLQAAIELRALRVLNFQRQLRMEFVQCTRRDTTLETALNIKLYKRTKRQGLREARATEKLEKQQKLEAERKRRQKHLEFLAAVLQHGKDLREFHRNNKAQLARMNKAVMNHHANAEREQKKEQERIEKERMRRLMAEDEEGYRKLIDQKKDKRLAFLLSQTDEYISNLTQMVKQHKDDQMKKKEEEGKRLIQFKKELLMSGEYIGIDEGSIVADMRVHVVEQCTGKKLTGDDAPMLKHLHRWLNMHPGWDWIDDEEDSCGSNDDHKPKVEEQPTATEDATDKAQATGNDEDAKDLITKAKVEDDEYRTEEQTYYSIAHTIHEKVVEQASIMVNGTLKEYQIKGLEWLVSLYNNNLNGILADEMGLGKTIQTISLVTYLMDRKKVMGPYLIIVPLSTLPNWVLEFEKWAPAVGVVSYKGSPQGRRLLQNQMRATKFNVLLTTYEYVIKDKAVLAKIQWKYMIIDEGHRMKNHHCKLTQVLNTHYIAPYRLLLTGTPLQNKLPELWALLNFLLPSIFKSCSTFEQWFNAPFATTGEKVELNEEETILIIRRLHKVLRPFLLRRLKKEVEHQLPDKVEYIIKCDMSALQRVLYKHMQSKGVLLTDGSEKGKHGKGGAKALMNTIVQLRKLCNHPFMFQHIEEKYCDHTGGHGVVSGPDLYRVSGKFELLDRILPKLKATNHRVLLFCQMTQCMTIIEDYLGWRQFGYLRLDGTTKAEDRGELLRKFNAKGSDVFVFLLSTRAGGLGLNLQTADTVVIFDSDWNPHQDLQAQDRAHRIGQRNEVRVLRLMTVNSVEERILAAARYKLNMDEKVIQAGMFDQKSTGSERQQFLQTILHQDDNEEEEENEVPDDEMINMMIARSEEEIEIFKRMDAERKKEDEEIHPGRERLIDESELPDWLTKDDDEVERFHYQYDEDTILGRGSRQRKEVDYTDSLTEKEWLKAIDDGAEFDEEEEEDDSKRKRRKRKNRKEESDDDSLILKRRRRQNLDKRSKKQMHKIMSAVIKHNQDGRTLSEPFMKLPSRQRLPDYYEIIKRPVDIKKILQRIEDCKYADLNELEKDFMQLCQNAQIYNEEASLIYLDSIALQKVFVGARQRITAAADAAAVAAGDNTGEAHGNGGSDNSDNDDDDGGDDGSDDEEIATTSAAAVKMKLKLNKSLASAPATPTQSSSNVSSGAATTSKKQTRRKRSQKKYTISDDDDDDMD

Target ORF information:

RefSeq Version	NM_080497.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster brahma (brm), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080497.5

ORF Insert Sequence:

ATGGCCTCGC CCTCTCCGGC GAACAGTCCC ATGCCACCGC CACAGGCGCC CAGTCCGATG 
GCCCCGCCCT CGCAGAGTCC CGCCCCCTCG CCGCACAGCC CGTATCCGCA CCAGCAGCCG 
GGTCCACTGC AAGGACCTCC GCCACCCGGA CATCCCGGTG CCTACGGACA TCCCATGCAG 
CACGGACCAC CCGGCCAGGG TCCACCCGGG CATCATATGC CACCGCACCA CCAGGGGATG 
AGTCCGCACA TGGGAATGCA GATGCCGCCG ACGGGACCGA ACATGTCCCC CTACCAAACC 
CACGGAATGC CACCCAATGC ACCCACACAA CCCTGCATAG TGTCACCTGG TGGCCCACCT 
GGAGGCCCGC CACCTCCGGA GCGCTCGAGC CAGGAGAACC TGCACGCGCT TCAACGCGCC 
ATTGATTCAA TGGAGGAAAA GGGCTTGCAA GAGGATCCTC GCTACTCGCA GCTGCTGGCA 
ATGCGAGCAA CCTCGAAGCA TCAGCACCTT AATGGCAATC AGGTTAACTT GTTGCGCACA 
CAGATCACCG CCTATCGGTT GCTGGCGCGG AACAAGCCCA TATCCATGCA GATGCAGCAG 
GCGTTGCAGG CCGCACAGCA ACAGCCACCG CCAGGACCTC CAATTGGGCC ACCTGGAGCA 
CCAGGCGGAC CGCCACCAGG GAGCCAGCAT GCAGGACAAC CGCCCGTGCC GCCGCAACAA 
CAGCAACAAC CGCCACCTTC AGCAGGAACT CCACCCCAGT GCTCCACGCC GCCCGCAAGC 
AATCCTTATG GACCGCCAGT TCCTGGCCAG AAAATGCAGG TAGCGCCACC ACCGCCACAC 
ATGCAACAGG GACAGCCATT GCCACCACAA CCGCCACAAG TGGGTGGACC CCCGCCTATA 
CAACAGCAGC AACCACCTCA GCAGCAGCAA CAACAGTCGC AGCCCCCGCC ACCGGAGCCA 
CATCAGCACC AGTTGCCAAA CGGAGGCAAA CCCCTGAGTA TGGGGCCATC TGGCGGGCAA 
CCACTAATAC CTTCATCACC CATGCAGCCA CAAGTGAGAG GGACACTGCC AGGAATGCCG 
CCAGGTAGTC AGGTTCCGCA ACCAGGAGGA GGTCCTCAGC GACAGGTACC ACCTGCGGGC 
ATGCCGATGC CCAAGCCCAA TCGCATTACA ACGGTGGCCA AACCCGTCGG TCTGGATCCC 
ATAACATTGC TGCAGGAAAG GGAGAATAGG ATAGCAGCCA GGATATCATT GCGCATGCAG 
GAGTTGCAGC GACTGCCGGC CACGATGTCC GAGGATCTGC GTCTGCAAGC GGCTATCGAG 
TTGAGGGCCC TAAGGGTCTT GAACTTCCAG CGACAGCTGC GTATGGAGTT TGTTCAGTGC 
ACGAGACGAG ATACCACTTT GGAAACAGCG TTAAACATTA AGCTCTACAA GAGGACCAAG 
AGGCAGGGCC TGCGGGAGGC ACGTGCCACC GAGAAGCTAG AGAAGCAACA GAAGCTAGAG 
GCTGAACGAA AGCGTCGCCA AAAGCATCTG GAGTTCTTGG CTGCTGTGCT TCAGCACGGC 
AAGGATCTAA GGGAGTTCCA CAGAAATAAT AAGGCCCAGC TTGCAAGGAT GAACAAGGCC 
GTGATGAATC ACCACGCCAA TGCCGAGCGT GAACAGAAGA AGGAACAAGA GCGCATCGAA 
AAGGAACGTA TGCGACGTCT GATGGCCGAG GATGAGGAGG GTTACCGCAA GCTGATTGAT 
CAAAAGAAGG ACAAGAGATT GGCTTTCCTA CTGTCGCAGA CAGATGAGTA TATTAGCAAC 
CTAACTCAGA TGGTGAAACA GCACAAGGAC GACCAGATGA AGAAAAAGGA GGAGGAGGGC 
AAGCGGCTGA TACAGTTCAA AAAGGAGCTG CTGATGAGCG GAGAGTACAT TGGCATTGAT 
GAGGGCAGCA TTGTTGCCGA TATGCGGGTA CATGTGGTGG AACAGTGCAC GGGTAAGAAA 
CTCACCGGCG ATGATGCACC CATGCTGAAG CACTTGCACC GCTGGCTGAA TATGCATCCT 
GGCTGGGATT GGATCGACGA CGAGGAGGAC AGCTGTGGGA GCAACGACGA TCATAAACCC 
AAGGTGGAGG AGCAACCAAC GGCAACAGAA GATGCCACCG ACAAAGCGCA GGCGACGGGC 
AACGATGAGG ATGCCAAGGA TCTAATTACC AAGGCCAAGG TGGAGGACGA TGAGTACAGG 
ACGGAGGAAC AGACGTACTA CAGCATCGCT CATACCATTC ATGAAAAGGT TGTGGAGCAG 
GCCAGCATCA TGGTTAATGG TACGCTCAAG GAATACCAGA TCAAAGGCTT GGAGTGGTTG 
GTTTCGCTGT ACAATAACAA TCTAAATGGT ATTTTGGCCG ATGAAATGGG TTTGGGTAAA 
ACCATTCAAA CCATTTCGCT GGTAACCTAC CTTATGGATC GAAAGAAGGT TATGGGTCCT 
TACTTGATTA TTGTACCACT CTCCACCCTG CCCAATTGGG TGCTGGAATT CGAGAAGTGG 
GCGCCAGCCG TGGGTGTAGT CAGCTACAAG GGCAGTCCGC AGGGAAGGCG ATTGCTGCAA 
AATCAAATGA GAGCAACCAA GTTCAATGTG CTGTTAACCA CATATGAGTA CGTGATTAAG 
GACAAGGCGG TGCTGGCCAA GATCCAGTGG AAATACATGA TTATCGACGA GGGTCACCGC 
ATGAAGAACC ATCACTGCAA ACTCACCCAG GTGCTGAACA CCCACTACAT TGCCCCCTAT 
CGGTTACTTC TCACGGGTAC ACCTCTGCAA AACAAGCTTC CCGAGTTGTG GGCGCTACTT 
AATTTCCTGC TGCCCTCGAT CTTTAAGTCC TGCTCCACAT TTGAACAGTG GTTCAATGCG 
CCATTTGCCA CCACTGGCGA GAAGGTCGAG CTAAACGAGG AGGAGACCAT CCTTATCATT 
CGTCGTCTGC ACAAGGTTCT TCGTCCTTTC CTGTTGCGTC GCCTCAAAAA GGAGGTAGAG 
CACCAGCTGC CCGACAAGGT CGAGTACATC ATCAAGTGCG ACATGTCCGC GCTGCAACGC 
GTTCTCTACA AGCACATGCA GAGCAAGGGT GTCCTGCTCA CCGATGGTTC CGAGAAGGGC 
AAGCACGGCA AGGGAGGCGC TAAGGCGCTG ATGAACACGA TCGTACAGCT GCGCAAGCTG 
TGCAACCATC CGTTTATGTT CCAGCACATA GAAGAGAAGT ACTGCGATCA CACTGGCGGC 
CACGGAGTTG TATCCGGACC GGATCTGTAT CGTGTCTCTG GCAAATTCGA GCTGCTTGAT 
CGCATCCTGC CCAAGCTGAA GGCGACCAAT CATCGCGTGC TGCTCTTCTG TCAGATGACC 
CAATGCATGA CCATCATCGA AGACTACCTG GGATGGCGCC AATTCGGCTA CCTTCGACTC 
GACGGTACCA CGAAGGCCGA GGATCGTGGC GAACTACTGC GAAAATTCAA CGCCAAAGGC 
TCGGATGTAT TTGTCTTTTT GCTATCTACC CGAGCTGGTG GTTTGGGTCT TAATCTGCAG 
ACCGCCGATA CTGTAGTAAT CTTCGACTCC GATTGGAATC CCCATCAGGA TCTGCAAGCC 
CAGGATCGTG CCCATCGTAT TGGTCAACGT AACGAGGTGC GCGTCCTGCG ACTAATGACA 
GTCAACTCGG TGGAAGAGCG CATCTTGGCA GCAGCCAGGT ACAAGCTGAA CATGGACGAG 
AAGGTCATCC AGGCTGGTAT GTTCGATCAG AAGTCAACGG GTAGCGAGCG ACAGCAGTTC 
CTACAAACGA TTCTGCACCA GGATGACAAC GAAGAGGAGG AGGAAAATGA GGTGCCCGAT 
GACGAAATGA TCAACATGAT GATCGCCCGC AGCGAGGAGG AGATTGAAAT ATTTAAGCGC 
ATGGATGCTG AGCGCAAGAA GGAGGATGAG GAGATCCATC CGGGTAGGGA ACGTCTGATC 
GATGAGTCCG AGCTACCCGA CTGGTTGACA AAGGATGATG ACGAAGTAGA GCGTTTCCAC 
TATCAGTACG ACGAGGATAC CATTCTAGGT CGAGGCTCCC GGCAGCGAAA GGAAGTAGAC 
TACACCGATA GTTTGACTGA AAAGGAGTGG CTGAAAGCCA TCGACGATGG CGCTGAGTTC 
GACGAGGAAG AGGAGGAAGA CGATTCGAAG CGCAAGCGAC GAAAGCGTAA GAATCGGAAG 
GAAGAGTCCG ACGATGATTC GTTGATCCTA AAACGGCGAC GACGCCAAAA TCTGGACAAG 
CGGTCCAAGA AGCAGATGCA CAAGATTATG AGTGCGGTTA TCAAGCACAA CCAAGATGGA 
CGAACCCTGT CCGAACCGTT CATGAAGTTG CCTTCGCGGC AGAGGTTGCC CGACTACTAC 
GAGATCATTA AGCGGCCAGT TGATATTAAG AAAATCCTGC AGCGCATTGA GGACTGCAAG 
TATGCCGATC TCAACGAGCT GGAGAAGGAC TTTATGCAGC TATGCCAGAA TGCACAGATC 
TACAACGAAG AGGCGTCTCT GATTTACCTG GACTCGATTG CCCTGCAAAA GGTATTCGTT 
GGGGCCAGGC AGAGGATAAC AGCTGCAGCG GATGCAGCCG CAGTGGCAGC CGGAGATAAC 
ACGGGTGAAG CGCACGGCAA TGGAGGTTCG GATAATTCGG ACAACGACGA CGACGATGGT 
GGCGATGACG GCTCCGACGA CGAGGAGATT GCAACCACTT CAGCGGCGGC TGTCAAAATG 
AAGCTGAAAC TGAACAAATC TCTGGCTAGT GCGCCCGCTA CGCCCACGCA ATCATCGTCG 
AATGTGAGCA GCGGAGCGGC TACCACATCC AAGAAGCAGA CGCGTCGCAA GCGCTCCCAA 
AAGAAGTACA CTATTTCAGA CGATGACGAC GATGACATGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

brm ( NM_168641.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730089.1 Drosophila melanogaster brahma (brm), transcript variant B, mRNA.

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CloneID	OFa20962
Clone ID Related Accession (Same CDS sequence)	NM_168641.1 , NM_080498.3
Accession Version	NM_168641.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	brahma, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCTCGC CCTCTCCGGC GAACAGTCCC ATGCCACCGC CACAGGCGCC CAGTCCGATG 
GCCCCGCCCT CGCAGAGTCC CGCCCCCTCG CCGCACAGCC CGTATCCGCA CCAGCAGCCG 
GGTCCACTGC AAGGACCTCC GCCACCCGGA CATCCCGGTG CCTACGGACA TCCCATGCAG 
CACGGACCAC CCGGCCAGGG TCCACCCGGG CATCATATGC CACCGCACCA CCAGGGGATG 
ATTTTCTCAA AAGGTCCGCA CATGGGAATG CAGATGCCGC CGACGGGACC GAACATGTCC 
CCCTACCAAA CCCACGGAAT GCCACCCAAT GCACCCACAC AACCCTGCAT AGTGTCACCT 
GGTGGCCCAC CTGGAGGCCC GCCACCTCCG GAGCGCTCGA GCCAGGAGAA CCTGCACGCG 
CTTCAACGCG CCATTGATTC AATGGAGGAA AAGGGCTTGC AAGAGGATCC TCGCTACTCG 
CAGCTGCTGG CAATGCGAGC AACCTCGAAG CATCAGCACC TTAATGGCAA TCAGGTTAAC 
TTGTTGCGCA CACAGATCAC CGCCTATCGG TTGCTGGCGC GGAACAAGCC CATATCCATG 
CAGATGCAGC AGGCGTTGCA GGCCGCACAG CAACAGCCAC CGCCAGGACC TCCAATTGGG 
CCACCTGGAG CACCAGGCGG ACCGCCACCA GGGAGCCAGC ATGCAGGACA ACCGCCCGTG 
CCGCCGCAAC AACAGCAACA ACCGCCACCT TCAGCAGGAA CTCCACCCCA GTGCTCCACG 
CCGCCCGCAA GCAATCCTTA TGGACCGCCA GTTCCTGGCC AGAAAATGCA GGTAGCGCCA 
CCACCGCCAC ACATGCAACA GGGACAGCCA TTGCCACCAC AACCGCCACA AGTGGGTGGA 
CCCCCGCCTA TACAACAGCA GCAACCACCT CAGCAGCAGC AACAACAGTC GCAGCCCCCG 
CCACCGGAGC CACATCAGCA CCAGTTGCCA AACGGAGGCA AACCCCTGAG TATGGGGCCA 
TCTGGCGGGC AACCACTAAT ACCTTCATCA CCCATGCAGC CACAAGTGAG AGGGACACTG 
CCAGGAATGC CGCCAGGTAG TCAGGTTCCG CAACCAGGAG GAGGTCCTCA GCGACAGGTA 
CCACCTGCGG GCATGCCGAT GCCCAAGCCC AATCGCATTA CAACGGTGGC CAAACCCGTC 
GGTCTGGATC CCATAACATT GCTGCAGGAA AGGGAGAATA GGATAGCAGC CAGGATATCA 
TTGCGCATGC AGGAGTTGCA GCGACTGCCG GCCACGATGT CCGAGGATCT GCGTCTGCAA 
GCGGCTATCG AGTTGAGGGC CCTAAGGGTC TTGAACTTCC AGCGACAGCT GCGTATGGAG 
TTTGTTCAGT GCACGAGACG AGATACCACT TTGGAAACAG CGTTAAACAT TAAGCTCTAC 
AAGAGGACCA AGAGGCAGGG CCTGCGGGAG GCACGTGCCA CCGAGAAGCT AGAGAAGCAA 
CAGAAGCTAG AGGCTGAACG AAAGCGTCGC CAAAAGCATC TGGAGTTCTT GGCTGCTGTG 
CTTCAGCACG GCAAGGATCT AAGGGAGTTC CACAGAAATA ATAAGGCCCA GCTTGCAAGG 
ATGAACAAGG CCGTGATGAA TCACCACGCC AATGCCGAGC GTGAACAGAA GAAGGAACAA 
GAGCGCATCG AAAAGGAACG TATGCGACGT CTGATGGCCG AGGATGAGGA GGGTTACCGC 
AAGCTGATTG ATCAAAAGAA GGACAAGAGA TTGGCTTTCC TACTGTCGCA GACAGATGAG 
TATATTAGCA ACCTAACTCA GATGGTGAAA CAGCACAAGG ACGACCAGAT GAAGAAAAAG 
GAGGAGGAGG GCAAGCGGCT GATACAGTTC AAAAAGGAGC TGCTGATGAG CGGAGAGTAC 
ATTGGCATTG ATGAGGGCAG CATTGTTGCC GATATGCGGG TACATGTGGT GGAACAGTGC 
ACGGGTAAGA AACTCACCGG CGATGATGCA CCCATGCTGA AGCACTTGCA CCGCTGGCTG 
AATATGCATC CTGGCTGGGA TTGGATCGAC GACGAGGAGG ACAGCTGTGG GAGCAACGAC 
GATCATAAAC CCAAGGTGGA GGAGCAACCA ACGGCAACAG AAGATGCCAC CGACAAAGCG 
CAGGCGACGG GCAACGATGA GGATGCCAAG GATCTAATTA CCAAGGCCAA GGTGGAGGAC 
GATGAGTACA GGACGGAGGA ACAGACGTAC TACAGCATCG CTCATACCAT TCATGAAAAG 
GTTGTGGAGC AGGCCAGCAT CATGGTTAAT GGTACGCTCA AGGAATACCA GATCAAAGGC 
TTGGAGTGGT TGGTTTCGCT GTACAATAAC AATCTAAATG GTATTTTGGC CGATGAAATG 
GGTTTGGGTA AAACCATTCA AACCATTTCG CTGGTAACCT ACCTTATGGA TCGAAAGAAG 
GTTATGGGTC CTTACTTGAT TATTGTACCA CTCTCCACCC TGCCCAATTG GGTGCTGGAA 
TTCGAGAAGT GGGCGCCAGC CGTGGGTGTA GTCAGCTACA AGGGCAGTCC GCAGGGAAGG 
CGATTGCTGC AAAATCAAAT GAGAGCAACC AAGTTCAATG TGCTGTTAAC CACATATGAG 
TACGTGATTA AGGACAAGGC GGTGCTGGCC AAGATCCAGT GGAAATACAT GATTATCGAC 
GAGGGTCACC GCATGAAGAA CCATCACTGC AAACTCACCC AGGTGCTGAA CACCCACTAC 
ATTGCCCCCT ATCGGTTACT TCTCACGGGT ACACCTCTGC AAAACAAGCT TCCCGAGTTG 
TGGGCGCTAC TTAATTTCCT GCTGCCCTCG ATCTTTAAGT CCTGCTCCAC ATTTGAACAG 
TGGTTCAATG CGCCATTTGC CACCACTGGC GAGAAGGTCG AGCTAAACGA GGAGGAGACC 
ATCCTTATCA TTCGTCGTCT GCACAAGGTT CTTCGTCCTT TCCTGTTGCG TCGCCTCAAA 
AAGGAGGTAG AGCACCAGCT GCCCGACAAG GTCGAGTACA TCATCAAGTG CGACATGTCC 
GCGCTGCAAC GCGTTCTCTA CAAGCACATG CAGAGCAAGG GTGTCCTGCT CACCGATGGT 
TCCGAGAAGG GCAAGCACGG CAAGGGAGGC GCTAAGGCGC TGATGAACAC GATCGTACAG 
CTGCGCAAGC TGTGCAACCA TCCGTTTATG TTCCAGCACA TAGAAGAGAA GTACTGCGAT 
CACACTGGCG GCCACGGAGT TGTATCCGGA CCGGATCTGT ATCGTGTCTC TGGCAAATTC 
GAGCTGCTTG ATCGCATCCT GCCCAAGCTG AAGGCGACCA ATCATCGCGT GCTGCTCTTC 
TGTCAGATGA CCCAATGCAT GACCATCATC GAAGACTACC TGGGATGGCG CCAATTCGGC 
TACCTTCGAC TCGACGGTAC CACGAAGGCC GAGGATCGTG GCGAACTACT GCGAAAATTC 
AACGCCAAAG GCTCGGATGT ATTTGTCTTT TTGCTATCTA CCCGAGCTGG TGGTTTGGGT 
CTTAATCTGC AGACCGCCGA TACTGTAGTA ATCTTCGACT CCGATTGGAA TCCCCATCAG 
GATCTGCAAG CCCAGGATCG TGCCCATCGT ATTGGTCAAC GTAACGAGGT GCGCGTCCTG 
CGACTAATGA CAGTCAACTC GGTGGAAGAG CGCATCTTGG CAGCAGCCAG GTACAAGCTG 
AACATGGACG AGAAGGTCAT CCAGGCTGGT ATGTTCGATC AGAAGTCAAC GGGTAGCGAG 
CGACAGCAGT TCCTACAAAC GATTCTGCAC CAGGATGACA ACGAAGAGGA GGAGGAAAAT 
GAGGTGCCCG ATGACGAAAT GATCAACATG ATGATCGCCC GCAGCGAGGA GGAGATTGAA 
ATATTTAAGC GCATGGATGC TGAGCGCAAG AAGGAGGATG AGGAGATCCA TCCGGGTAGG 
GAACGTCTGA TCGATGAGTC CGAGCTACCC GACTGGTTGA CAAAGGATGA TGACGAAGTA 
GAGCGTTTCC ACTATCAGTA CGACGAGGAT ACCATTCTAG GTCGAGGCTC CCGGCAGCGA 
AAGGAAGTAG ACTACACCGA TAGTTTGACT GAAAAGGAGT GGCTGAAAGC CATCGACGAT 
GGCGCTGAGT TCGACGAGGA AGAGGAGGAA GACGATTCGA AGCGCAAGCG ACGAAAGCGT 
AAGAATCGGA AGGAAGAGTC CGACGATGAT TCGTTGATCC TAAAACGGCG ACGACGCCAA 
AATCTGGACA AGCGGTCCAA GAAGCAGATG CACAAGATTA TGAGTGCGGT TATCAAGCAC 
AACCAAGATG GACGAACCCT GTCCGAACCG TTCATGAAGT TGCCTTCGCG GCAGAGGTTG 
CCCGACTACT ACGAGATCAT TAAGCGGCCA GTTGATATTA AGAAAATCCT GCAGCGCATT 
GAGGACTGCA AGTATGCCGA TCTCAACGAG CTGGAGAAGG ACTTTATGCA GCTATGCCAG 
AATGCACAGA TCTACAACGA AGAGGCGTCT CTGATTTACC TGGACTCGAT TGCCCTGCAA 
AAGGTATTCG TTGGGGCCAG GCAGAGGATA ACAGCTGCAG CGGATGCAGC CGCAGTGGCA 
GCCGGAGATA ACACGGGTGA AGCGCACGGC AATGGAGGTT CGGATAATTC GGACAACGAC 
GACGACGATG GTGGCGATGA CGGCTCCGAC GACGAGGAGA TTGCAACCAC TTCAGCGGCG 
GCTGTCAAAA TGAAGCTGAA ACTGAACAAA TCTCTGGCTA GTGCGCCCGC TACGCCCACG 
CAATCATCGT CGAATGTGAG CAGCGGAGCG GCTACCACAT CCAAGAAGCA GACGCGTCGC 
AAGCGCTCCC AAAAGAAGTA CACTATTTCA GACGATGACG ACGATGACAT GGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730089.1
CDS	342..5258
Translation	MASPSPANSPMPPPQAPSPMAPPSQSPAPSPHSPYPHQQPGPLQGPPPPGHPGAYGHPMQHGPPGQGPPGHHMPPHHQGMIFSKGPHMGMQMPPTGPNMSPYQTHGMPPNAPTQPCIVSPGGPPGGPPPPERSSQENLHALQRAIDSMEEKGLQEDPRYSQLLAMRATSKHQHLNGNQVNLLRTQITAYRLLARNKPISMQMQQALQAAQQQPPPGPPIGPPGAPGGPPPGSQHAGQPPVPPQQQQQPPPSAGTPPQCSTPPASNPYGPPVPGQKMQVAPPPPHMQQGQPLPPQPPQVGGPPPIQQQQPPQQQQQQSQPPPPEPHQHQLPNGGKPLSMGPSGGQPLIPSSPMQPQVRGTLPGMPPGSQVPQPGGGPQRQVPPAGMPMPKPNRITTVAKPVGLDPITLLQERENRIAARISLRMQELQRLPATMSEDLRLQAAIELRALRVLNFQRQLRMEFVQCTRRDTTLETALNIKLYKRTKRQGLREARATEKLEKQQKLEAERKRRQKHLEFLAAVLQHGKDLREFHRNNKAQLARMNKAVMNHHANAEREQKKEQERIEKERMRRLMAEDEEGYRKLIDQKKDKRLAFLLSQTDEYISNLTQMVKQHKDDQMKKKEEEGKRLIQFKKELLMSGEYIGIDEGSIVADMRVHVVEQCTGKKLTGDDAPMLKHLHRWLNMHPGWDWIDDEEDSCGSNDDHKPKVEEQPTATEDATDKAQATGNDEDAKDLITKAKVEDDEYRTEEQTYYSIAHTIHEKVVEQASIMVNGTLKEYQIKGLEWLVSLYNNNLNGILADEMGLGKTIQTISLVTYLMDRKKVMGPYLIIVPLSTLPNWVLEFEKWAPAVGVVSYKGSPQGRRLLQNQMRATKFNVLLTTYEYVIKDKAVLAKIQWKYMIIDEGHRMKNHHCKLTQVLNTHYIAPYRLLLTGTPLQNKLPELWALLNFLLPSIFKSCSTFEQWFNAPFATTGEKVELNEEETILIIRRLHKVLRPFLLRRLKKEVEHQLPDKVEYIIKCDMSALQRVLYKHMQSKGVLLTDGSEKGKHGKGGAKALMNTIVQLRKLCNHPFMFQHIEEKYCDHTGGHGVVSGPDLYRVSGKFELLDRILPKLKATNHRVLLFCQMTQCMTIIEDYLGWRQFGYLRLDGTTKAEDRGELLRKFNAKGSDVFVFLLSTRAGGLGLNLQTADTVVIFDSDWNPHQDLQAQDRAHRIGQRNEVRVLRLMTVNSVEERILAAARYKLNMDEKVIQAGMFDQKSTGSERQQFLQTILHQDDNEEEEENEVPDDEMINMMIARSEEEIEIFKRMDAERKKEDEEIHPGRERLIDESELPDWLTKDDDEVERFHYQYDEDTILGRGSRQRKEVDYTDSLTEKEWLKAIDDGAEFDEEEEEDDSKRKRRKRKNRKEESDDDSLILKRRRRQNLDKRSKKQMHKIMSAVIKHNQDGRTLSEPFMKLPSRQRLPDYYEIIKRPVDIKKILQRIEDCKYADLNELEKDFMQLCQNAQIYNEEASLIYLDSIALQKVFVGARQRITAAADAAAVAAGDNTGEAHGNGGSDNSDNDDDDGGDDGSDDEEIATTSAAAVKMKLKLNKSLASAPATPTQSSSNVSSGAATTSKKQTRRKRSQKKYTISDDDDDDMD

Target ORF information:

RefSeq Version	NM_168641.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster brahma (brm), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168641.1

ORF Insert Sequence:

ATGGCCTCGC CCTCTCCGGC GAACAGTCCC ATGCCACCGC CACAGGCGCC CAGTCCGATG 
GCCCCGCCCT CGCAGAGTCC CGCCCCCTCG CCGCACAGCC CGTATCCGCA CCAGCAGCCG 
GGTCCACTGC AAGGACCTCC GCCACCCGGA CATCCCGGTG CCTACGGACA TCCCATGCAG 
CACGGACCAC CCGGCCAGGG TCCACCCGGG CATCATATGC CACCGCACCA CCAGGGGATG 
ATTTTCTCAA AAGGTCCGCA CATGGGAATG CAGATGCCGC CGACGGGACC GAACATGTCC 
CCCTACCAAA CCCACGGAAT GCCACCCAAT GCACCCACAC AACCCTGCAT AGTGTCACCT 
GGTGGCCCAC CTGGAGGCCC GCCACCTCCG GAGCGCTCGA GCCAGGAGAA CCTGCACGCG 
CTTCAACGCG CCATTGATTC AATGGAGGAA AAGGGCTTGC AAGAGGATCC TCGCTACTCG 
CAGCTGCTGG CAATGCGAGC AACCTCGAAG CATCAGCACC TTAATGGCAA TCAGGTTAAC 
TTGTTGCGCA CACAGATCAC CGCCTATCGG TTGCTGGCGC GGAACAAGCC CATATCCATG 
CAGATGCAGC AGGCGTTGCA GGCCGCACAG CAACAGCCAC CGCCAGGACC TCCAATTGGG 
CCACCTGGAG CACCAGGCGG ACCGCCACCA GGGAGCCAGC ATGCAGGACA ACCGCCCGTG 
CCGCCGCAAC AACAGCAACA ACCGCCACCT TCAGCAGGAA CTCCACCCCA GTGCTCCACG 
CCGCCCGCAA GCAATCCTTA TGGACCGCCA GTTCCTGGCC AGAAAATGCA GGTAGCGCCA 
CCACCGCCAC ACATGCAACA GGGACAGCCA TTGCCACCAC AACCGCCACA AGTGGGTGGA 
CCCCCGCCTA TACAACAGCA GCAACCACCT CAGCAGCAGC AACAACAGTC GCAGCCCCCG 
CCACCGGAGC CACATCAGCA CCAGTTGCCA AACGGAGGCA AACCCCTGAG TATGGGGCCA 
TCTGGCGGGC AACCACTAAT ACCTTCATCA CCCATGCAGC CACAAGTGAG AGGGACACTG 
CCAGGAATGC CGCCAGGTAG TCAGGTTCCG CAACCAGGAG GAGGTCCTCA GCGACAGGTA 
CCACCTGCGG GCATGCCGAT GCCCAAGCCC AATCGCATTA CAACGGTGGC CAAACCCGTC 
GGTCTGGATC CCATAACATT GCTGCAGGAA AGGGAGAATA GGATAGCAGC CAGGATATCA 
TTGCGCATGC AGGAGTTGCA GCGACTGCCG GCCACGATGT CCGAGGATCT GCGTCTGCAA 
GCGGCTATCG AGTTGAGGGC CCTAAGGGTC TTGAACTTCC AGCGACAGCT GCGTATGGAG 
TTTGTTCAGT GCACGAGACG AGATACCACT TTGGAAACAG CGTTAAACAT TAAGCTCTAC 
AAGAGGACCA AGAGGCAGGG CCTGCGGGAG GCACGTGCCA CCGAGAAGCT AGAGAAGCAA 
CAGAAGCTAG AGGCTGAACG AAAGCGTCGC CAAAAGCATC TGGAGTTCTT GGCTGCTGTG 
CTTCAGCACG GCAAGGATCT AAGGGAGTTC CACAGAAATA ATAAGGCCCA GCTTGCAAGG 
ATGAACAAGG CCGTGATGAA TCACCACGCC AATGCCGAGC GTGAACAGAA GAAGGAACAA 
GAGCGCATCG AAAAGGAACG TATGCGACGT CTGATGGCCG AGGATGAGGA GGGTTACCGC 
AAGCTGATTG ATCAAAAGAA GGACAAGAGA TTGGCTTTCC TACTGTCGCA GACAGATGAG 
TATATTAGCA ACCTAACTCA GATGGTGAAA CAGCACAAGG ACGACCAGAT GAAGAAAAAG 
GAGGAGGAGG GCAAGCGGCT GATACAGTTC AAAAAGGAGC TGCTGATGAG CGGAGAGTAC 
ATTGGCATTG ATGAGGGCAG CATTGTTGCC GATATGCGGG TACATGTGGT GGAACAGTGC 
ACGGGTAAGA AACTCACCGG CGATGATGCA CCCATGCTGA AGCACTTGCA CCGCTGGCTG 
AATATGCATC CTGGCTGGGA TTGGATCGAC GACGAGGAGG ACAGCTGTGG GAGCAACGAC 
GATCATAAAC CCAAGGTGGA GGAGCAACCA ACGGCAACAG AAGATGCCAC CGACAAAGCG 
CAGGCGACGG GCAACGATGA GGATGCCAAG GATCTAATTA CCAAGGCCAA GGTGGAGGAC 
GATGAGTACA GGACGGAGGA ACAGACGTAC TACAGCATCG CTCATACCAT TCATGAAAAG 
GTTGTGGAGC AGGCCAGCAT CATGGTTAAT GGTACGCTCA AGGAATACCA GATCAAAGGC 
TTGGAGTGGT TGGTTTCGCT GTACAATAAC AATCTAAATG GTATTTTGGC CGATGAAATG 
GGTTTGGGTA AAACCATTCA AACCATTTCG CTGGTAACCT ACCTTATGGA TCGAAAGAAG 
GTTATGGGTC CTTACTTGAT TATTGTACCA CTCTCCACCC TGCCCAATTG GGTGCTGGAA 
TTCGAGAAGT GGGCGCCAGC CGTGGGTGTA GTCAGCTACA AGGGCAGTCC GCAGGGAAGG 
CGATTGCTGC AAAATCAAAT GAGAGCAACC AAGTTCAATG TGCTGTTAAC CACATATGAG 
TACGTGATTA AGGACAAGGC GGTGCTGGCC AAGATCCAGT GGAAATACAT GATTATCGAC 
GAGGGTCACC GCATGAAGAA CCATCACTGC AAACTCACCC AGGTGCTGAA CACCCACTAC 
ATTGCCCCCT ATCGGTTACT TCTCACGGGT ACACCTCTGC AAAACAAGCT TCCCGAGTTG 
TGGGCGCTAC TTAATTTCCT GCTGCCCTCG ATCTTTAAGT CCTGCTCCAC ATTTGAACAG 
TGGTTCAATG CGCCATTTGC CACCACTGGC GAGAAGGTCG AGCTAAACGA GGAGGAGACC 
ATCCTTATCA TTCGTCGTCT GCACAAGGTT CTTCGTCCTT TCCTGTTGCG TCGCCTCAAA 
AAGGAGGTAG AGCACCAGCT GCCCGACAAG GTCGAGTACA TCATCAAGTG CGACATGTCC 
GCGCTGCAAC GCGTTCTCTA CAAGCACATG CAGAGCAAGG GTGTCCTGCT CACCGATGGT 
TCCGAGAAGG GCAAGCACGG CAAGGGAGGC GCTAAGGCGC TGATGAACAC GATCGTACAG 
CTGCGCAAGC TGTGCAACCA TCCGTTTATG TTCCAGCACA TAGAAGAGAA GTACTGCGAT 
CACACTGGCG GCCACGGAGT TGTATCCGGA CCGGATCTGT ATCGTGTCTC TGGCAAATTC 
GAGCTGCTTG ATCGCATCCT GCCCAAGCTG AAGGCGACCA ATCATCGCGT GCTGCTCTTC 
TGTCAGATGA CCCAATGCAT GACCATCATC GAAGACTACC TGGGATGGCG CCAATTCGGC 
TACCTTCGAC TCGACGGTAC CACGAAGGCC GAGGATCGTG GCGAACTACT GCGAAAATTC 
AACGCCAAAG GCTCGGATGT ATTTGTCTTT TTGCTATCTA CCCGAGCTGG TGGTTTGGGT 
CTTAATCTGC AGACCGCCGA TACTGTAGTA ATCTTCGACT CCGATTGGAA TCCCCATCAG 
GATCTGCAAG CCCAGGATCG TGCCCATCGT ATTGGTCAAC GTAACGAGGT GCGCGTCCTG 
CGACTAATGA CAGTCAACTC GGTGGAAGAG CGCATCTTGG CAGCAGCCAG GTACAAGCTG 
AACATGGACG AGAAGGTCAT CCAGGCTGGT ATGTTCGATC AGAAGTCAAC GGGTAGCGAG 
CGACAGCAGT TCCTACAAAC GATTCTGCAC CAGGATGACA ACGAAGAGGA GGAGGAAAAT 
GAGGTGCCCG ATGACGAAAT GATCAACATG ATGATCGCCC GCAGCGAGGA GGAGATTGAA 
ATATTTAAGC GCATGGATGC TGAGCGCAAG AAGGAGGATG AGGAGATCCA TCCGGGTAGG 
GAACGTCTGA TCGATGAGTC CGAGCTACCC GACTGGTTGA CAAAGGATGA TGACGAAGTA 
GAGCGTTTCC ACTATCAGTA CGACGAGGAT ACCATTCTAG GTCGAGGCTC CCGGCAGCGA 
AAGGAAGTAG ACTACACCGA TAGTTTGACT GAAAAGGAGT GGCTGAAAGC CATCGACGAT 
GGCGCTGAGT TCGACGAGGA AGAGGAGGAA GACGATTCGA AGCGCAAGCG ACGAAAGCGT 
AAGAATCGGA AGGAAGAGTC CGACGATGAT TCGTTGATCC TAAAACGGCG ACGACGCCAA 
AATCTGGACA AGCGGTCCAA GAAGCAGATG CACAAGATTA TGAGTGCGGT TATCAAGCAC 
AACCAAGATG GACGAACCCT GTCCGAACCG TTCATGAAGT TGCCTTCGCG GCAGAGGTTG 
CCCGACTACT ACGAGATCAT TAAGCGGCCA GTTGATATTA AGAAAATCCT GCAGCGCATT 
GAGGACTGCA AGTATGCCGA TCTCAACGAG CTGGAGAAGG ACTTTATGCA GCTATGCCAG 
AATGCACAGA TCTACAACGA AGAGGCGTCT CTGATTTACC TGGACTCGAT TGCCCTGCAA 
AAGGTATTCG TTGGGGCCAG GCAGAGGATA ACAGCTGCAG CGGATGCAGC CGCAGTGGCA 
GCCGGAGATA ACACGGGTGA AGCGCACGGC AATGGAGGTT CGGATAATTC GGACAACGAC 
GACGACGATG GTGGCGATGA CGGCTCCGAC GACGAGGAGA TTGCAACCAC TTCAGCGGCG 
GCTGTCAAAA TGAAGCTGAA ACTGAACAAA TCTCTGGCTA GTGCGCCCGC TACGCCCACG 
CAATCATCGT CGAATGTGAG CAGCGGAGCG GCTACCACAT CCAAGAAGCA GACGCGTCGC 
AAGCGCTCCC AAAAGAAGTA CACTATTTCA GACGATGACG ACGATGACAT GGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

brm ( NM_080498.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_536746.1 Drosophila melanogaster brahma (brm), transcript variant A, mRNA.

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CloneID	OFa20962
Clone ID Related Accession (Same CDS sequence)	NM_168641.1 , NM_080498.3
Accession Version	NM_080498.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	brahma, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_080498.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCTCGC CCTCTCCGGC GAACAGTCCC ATGCCACCGC CACAGGCGCC CAGTCCGATG 
GCCCCGCCCT CGCAGAGTCC CGCCCCCTCG CCGCACAGCC CGTATCCGCA CCAGCAGCCG 
GGTCCACTGC AAGGACCTCC GCCACCCGGA CATCCCGGTG CCTACGGACA TCCCATGCAG 
CACGGACCAC CCGGCCAGGG TCCACCCGGG CATCATATGC CACCGCACCA CCAGGGGATG 
ATTTTCTCAA AAGGTCCGCA CATGGGAATG CAGATGCCGC CGACGGGACC GAACATGTCC 
CCCTACCAAA CCCACGGAAT GCCACCCAAT GCACCCACAC AACCCTGCAT AGTGTCACCT 
GGTGGCCCAC CTGGAGGCCC GCCACCTCCG GAGCGCTCGA GCCAGGAGAA CCTGCACGCG 
CTTCAACGCG CCATTGATTC AATGGAGGAA AAGGGCTTGC AAGAGGATCC TCGCTACTCG 
CAGCTGCTGG CAATGCGAGC AACCTCGAAG CATCAGCACC TTAATGGCAA TCAGGTTAAC 
TTGTTGCGCA CACAGATCAC CGCCTATCGG TTGCTGGCGC GGAACAAGCC CATATCCATG 
CAGATGCAGC AGGCGTTGCA GGCCGCACAG CAACAGCCAC CGCCAGGACC TCCAATTGGG 
CCACCTGGAG CACCAGGCGG ACCGCCACCA GGGAGCCAGC ATGCAGGACA ACCGCCCGTG 
CCGCCGCAAC AACAGCAACA ACCGCCACCT TCAGCAGGAA CTCCACCCCA GTGCTCCACG 
CCGCCCGCAA GCAATCCTTA TGGACCGCCA GTTCCTGGCC AGAAAATGCA GGTAGCGCCA 
CCACCGCCAC ACATGCAACA GGGACAGCCA TTGCCACCAC AACCGCCACA AGTGGGTGGA 
CCCCCGCCTA TACAACAGCA GCAACCACCT CAGCAGCAGC AACAACAGTC GCAGCCCCCG 
CCACCGGAGC CACATCAGCA CCAGTTGCCA AACGGAGGCA AACCCCTGAG TATGGGGCCA 
TCTGGCGGGC AACCACTAAT ACCTTCATCA CCCATGCAGC CACAAGTGAG AGGGACACTG 
CCAGGAATGC CGCCAGGTAG TCAGGTTCCG CAACCAGGAG GAGGTCCTCA GCGACAGGTA 
CCACCTGCGG GCATGCCGAT GCCCAAGCCC AATCGCATTA CAACGGTGGC CAAACCCGTC 
GGTCTGGATC CCATAACATT GCTGCAGGAA AGGGAGAATA GGATAGCAGC CAGGATATCA 
TTGCGCATGC AGGAGTTGCA GCGACTGCCG GCCACGATGT CCGAGGATCT GCGTCTGCAA 
GCGGCTATCG AGTTGAGGGC CCTAAGGGTC TTGAACTTCC AGCGACAGCT GCGTATGGAG 
TTTGTTCAGT GCACGAGACG AGATACCACT TTGGAAACAG CGTTAAACAT TAAGCTCTAC 
AAGAGGACCA AGAGGCAGGG CCTGCGGGAG GCACGTGCCA CCGAGAAGCT AGAGAAGCAA 
CAGAAGCTAG AGGCTGAACG AAAGCGTCGC CAAAAGCATC TGGAGTTCTT GGCTGCTGTG 
CTTCAGCACG GCAAGGATCT AAGGGAGTTC CACAGAAATA ATAAGGCCCA GCTTGCAAGG 
ATGAACAAGG CCGTGATGAA TCACCACGCC AATGCCGAGC GTGAACAGAA GAAGGAACAA 
GAGCGCATCG AAAAGGAACG TATGCGACGT CTGATGGCCG AGGATGAGGA GGGTTACCGC 
AAGCTGATTG ATCAAAAGAA GGACAAGAGA TTGGCTTTCC TACTGTCGCA GACAGATGAG 
TATATTAGCA ACCTAACTCA GATGGTGAAA CAGCACAAGG ACGACCAGAT GAAGAAAAAG 
GAGGAGGAGG GCAAGCGGCT GATACAGTTC AAAAAGGAGC TGCTGATGAG CGGAGAGTAC 
ATTGGCATTG ATGAGGGCAG CATTGTTGCC GATATGCGGG TACATGTGGT GGAACAGTGC 
ACGGGTAAGA AACTCACCGG CGATGATGCA CCCATGCTGA AGCACTTGCA CCGCTGGCTG 
AATATGCATC CTGGCTGGGA TTGGATCGAC GACGAGGAGG ACAGCTGTGG GAGCAACGAC 
GATCATAAAC CCAAGGTGGA GGAGCAACCA ACGGCAACAG AAGATGCCAC CGACAAAGCG 
CAGGCGACGG GCAACGATGA GGATGCCAAG GATCTAATTA CCAAGGCCAA GGTGGAGGAC 
GATGAGTACA GGACGGAGGA ACAGACGTAC TACAGCATCG CTCATACCAT TCATGAAAAG 
GTTGTGGAGC AGGCCAGCAT CATGGTTAAT GGTACGCTCA AGGAATACCA GATCAAAGGC 
TTGGAGTGGT TGGTTTCGCT GTACAATAAC AATCTAAATG GTATTTTGGC CGATGAAATG 
GGTTTGGGTA AAACCATTCA AACCATTTCG CTGGTAACCT ACCTTATGGA TCGAAAGAAG 
GTTATGGGTC CTTACTTGAT TATTGTACCA CTCTCCACCC TGCCCAATTG GGTGCTGGAA 
TTCGAGAAGT GGGCGCCAGC CGTGGGTGTA GTCAGCTACA AGGGCAGTCC GCAGGGAAGG 
CGATTGCTGC AAAATCAAAT GAGAGCAACC AAGTTCAATG TGCTGTTAAC CACATATGAG 
TACGTGATTA AGGACAAGGC GGTGCTGGCC AAGATCCAGT GGAAATACAT GATTATCGAC 
GAGGGTCACC GCATGAAGAA CCATCACTGC AAACTCACCC AGGTGCTGAA CACCCACTAC 
ATTGCCCCCT ATCGGTTACT TCTCACGGGT ACACCTCTGC AAAACAAGCT TCCCGAGTTG 
TGGGCGCTAC TTAATTTCCT GCTGCCCTCG ATCTTTAAGT CCTGCTCCAC ATTTGAACAG 
TGGTTCAATG CGCCATTTGC CACCACTGGC GAGAAGGTCG AGCTAAACGA GGAGGAGACC 
ATCCTTATCA TTCGTCGTCT GCACAAGGTT CTTCGTCCTT TCCTGTTGCG TCGCCTCAAA 
AAGGAGGTAG AGCACCAGCT GCCCGACAAG GTCGAGTACA TCATCAAGTG CGACATGTCC 
GCGCTGCAAC GCGTTCTCTA CAAGCACATG CAGAGCAAGG GTGTCCTGCT CACCGATGGT 
TCCGAGAAGG GCAAGCACGG CAAGGGAGGC GCTAAGGCGC TGATGAACAC GATCGTACAG 
CTGCGCAAGC TGTGCAACCA TCCGTTTATG TTCCAGCACA TAGAAGAGAA GTACTGCGAT 
CACACTGGCG GCCACGGAGT TGTATCCGGA CCGGATCTGT ATCGTGTCTC TGGCAAATTC 
GAGCTGCTTG ATCGCATCCT GCCCAAGCTG AAGGCGACCA ATCATCGCGT GCTGCTCTTC 
TGTCAGATGA CCCAATGCAT GACCATCATC GAAGACTACC TGGGATGGCG CCAATTCGGC 
TACCTTCGAC TCGACGGTAC CACGAAGGCC GAGGATCGTG GCGAACTACT GCGAAAATTC 
AACGCCAAAG GCTCGGATGT ATTTGTCTTT TTGCTATCTA CCCGAGCTGG TGGTTTGGGT 
CTTAATCTGC AGACCGCCGA TACTGTAGTA ATCTTCGACT CCGATTGGAA TCCCCATCAG 
GATCTGCAAG CCCAGGATCG TGCCCATCGT ATTGGTCAAC GTAACGAGGT GCGCGTCCTG 
CGACTAATGA CAGTCAACTC GGTGGAAGAG CGCATCTTGG CAGCAGCCAG GTACAAGCTG 
AACATGGACG AGAAGGTCAT CCAGGCTGGT ATGTTCGATC AGAAGTCAAC GGGTAGCGAG 
CGACAGCAGT TCCTACAAAC GATTCTGCAC CAGGATGACA ACGAAGAGGA GGAGGAAAAT 
GAGGTGCCCG ATGACGAAAT GATCAACATG ATGATCGCCC GCAGCGAGGA GGAGATTGAA 
ATATTTAAGC GCATGGATGC TGAGCGCAAG AAGGAGGATG AGGAGATCCA TCCGGGTAGG 
GAACGTCTGA TCGATGAGTC CGAGCTACCC GACTGGTTGA CAAAGGATGA TGACGAAGTA 
GAGCGTTTCC ACTATCAGTA CGACGAGGAT ACCATTCTAG GTCGAGGCTC CCGGCAGCGA 
AAGGAAGTAG ACTACACCGA TAGTTTGACT GAAAAGGAGT GGCTGAAAGC CATCGACGAT 
GGCGCTGAGT TCGACGAGGA AGAGGAGGAA GACGATTCGA AGCGCAAGCG ACGAAAGCGT 
AAGAATCGGA AGGAAGAGTC CGACGATGAT TCGTTGATCC TAAAACGGCG ACGACGCCAA 
AATCTGGACA AGCGGTCCAA GAAGCAGATG CACAAGATTA TGAGTGCGGT TATCAAGCAC 
AACCAAGATG GACGAACCCT GTCCGAACCG TTCATGAAGT TGCCTTCGCG GCAGAGGTTG 
CCCGACTACT ACGAGATCAT TAAGCGGCCA GTTGATATTA AGAAAATCCT GCAGCGCATT 
GAGGACTGCA AGTATGCCGA TCTCAACGAG CTGGAGAAGG ACTTTATGCA GCTATGCCAG 
AATGCACAGA TCTACAACGA AGAGGCGTCT CTGATTTACC TGGACTCGAT TGCCCTGCAA 
AAGGTATTCG TTGGGGCCAG GCAGAGGATA ACAGCTGCAG CGGATGCAGC CGCAGTGGCA 
GCCGGAGATA ACACGGGTGA AGCGCACGGC AATGGAGGTT CGGATAATTC GGACAACGAC 
GACGACGATG GTGGCGATGA CGGCTCCGAC GACGAGGAGA TTGCAACCAC TTCAGCGGCG 
GCTGTCAAAA TGAAGCTGAA ACTGAACAAA TCTCTGGCTA GTGCGCCCGC TACGCCCACG 
CAATCATCGT CGAATGTGAG CAGCGGAGCG GCTACCACAT CCAAGAAGCA GACGCGTCGC 
AAGCGCTCCC AAAAGAAGTA CACTATTTCA GACGATGACG ACGATGACAT GGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_536746.1
CDS	128..5044
Translation	MASPSPANSPMPPPQAPSPMAPPSQSPAPSPHSPYPHQQPGPLQGPPPPGHPGAYGHPMQHGPPGQGPPGHHMPPHHQGMIFSKGPHMGMQMPPTGPNMSPYQTHGMPPNAPTQPCIVSPGGPPGGPPPPERSSQENLHALQRAIDSMEEKGLQEDPRYSQLLAMRATSKHQHLNGNQVNLLRTQITAYRLLARNKPISMQMQQALQAAQQQPPPGPPIGPPGAPGGPPPGSQHAGQPPVPPQQQQQPPPSAGTPPQCSTPPASNPYGPPVPGQKMQVAPPPPHMQQGQPLPPQPPQVGGPPPIQQQQPPQQQQQQSQPPPPEPHQHQLPNGGKPLSMGPSGGQPLIPSSPMQPQVRGTLPGMPPGSQVPQPGGGPQRQVPPAGMPMPKPNRITTVAKPVGLDPITLLQERENRIAARISLRMQELQRLPATMSEDLRLQAAIELRALRVLNFQRQLRMEFVQCTRRDTTLETALNIKLYKRTKRQGLREARATEKLEKQQKLEAERKRRQKHLEFLAAVLQHGKDLREFHRNNKAQLARMNKAVMNHHANAEREQKKEQERIEKERMRRLMAEDEEGYRKLIDQKKDKRLAFLLSQTDEYISNLTQMVKQHKDDQMKKKEEEGKRLIQFKKELLMSGEYIGIDEGSIVADMRVHVVEQCTGKKLTGDDAPMLKHLHRWLNMHPGWDWIDDEEDSCGSNDDHKPKVEEQPTATEDATDKAQATGNDEDAKDLITKAKVEDDEYRTEEQTYYSIAHTIHEKVVEQASIMVNGTLKEYQIKGLEWLVSLYNNNLNGILADEMGLGKTIQTISLVTYLMDRKKVMGPYLIIVPLSTLPNWVLEFEKWAPAVGVVSYKGSPQGRRLLQNQMRATKFNVLLTTYEYVIKDKAVLAKIQWKYMIIDEGHRMKNHHCKLTQVLNTHYIAPYRLLLTGTPLQNKLPELWALLNFLLPSIFKSCSTFEQWFNAPFATTGEKVELNEEETILIIRRLHKVLRPFLLRRLKKEVEHQLPDKVEYIIKCDMSALQRVLYKHMQSKGVLLTDGSEKGKHGKGGAKALMNTIVQLRKLCNHPFMFQHIEEKYCDHTGGHGVVSGPDLYRVSGKFELLDRILPKLKATNHRVLLFCQMTQCMTIIEDYLGWRQFGYLRLDGTTKAEDRGELLRKFNAKGSDVFVFLLSTRAGGLGLNLQTADTVVIFDSDWNPHQDLQAQDRAHRIGQRNEVRVLRLMTVNSVEERILAAARYKLNMDEKVIQAGMFDQKSTGSERQQFLQTILHQDDNEEEEENEVPDDEMINMMIARSEEEIEIFKRMDAERKKEDEEIHPGRERLIDESELPDWLTKDDDEVERFHYQYDEDTILGRGSRQRKEVDYTDSLTEKEWLKAIDDGAEFDEEEEEDDSKRKRRKRKNRKEESDDDSLILKRRRRQNLDKRSKKQMHKIMSAVIKHNQDGRTLSEPFMKLPSRQRLPDYYEIIKRPVDIKKILQRIEDCKYADLNELEKDFMQLCQNAQIYNEEASLIYLDSIALQKVFVGARQRITAAADAAAVAAGDNTGEAHGNGGSDNSDNDDDDGGDDGSDDEEIATTSAAAVKMKLKLNKSLASAPATPTQSSSNVSSGAATTSKKQTRRKRSQKKYTISDDDDDDMD

Target ORF information:

RefSeq Version	NM_080498.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster brahma (brm), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080498.3

ORF Insert Sequence:

ATGGCCTCGC CCTCTCCGGC GAACAGTCCC ATGCCACCGC CACAGGCGCC CAGTCCGATG 
GCCCCGCCCT CGCAGAGTCC CGCCCCCTCG CCGCACAGCC CGTATCCGCA CCAGCAGCCG 
GGTCCACTGC AAGGACCTCC GCCACCCGGA CATCCCGGTG CCTACGGACA TCCCATGCAG 
CACGGACCAC CCGGCCAGGG TCCACCCGGG CATCATATGC CACCGCACCA CCAGGGGATG 
ATTTTCTCAA AAGGTCCGCA CATGGGAATG CAGATGCCGC CGACGGGACC GAACATGTCC 
CCCTACCAAA CCCACGGAAT GCCACCCAAT GCACCCACAC AACCCTGCAT AGTGTCACCT 
GGTGGCCCAC CTGGAGGCCC GCCACCTCCG GAGCGCTCGA GCCAGGAGAA CCTGCACGCG 
CTTCAACGCG CCATTGATTC AATGGAGGAA AAGGGCTTGC AAGAGGATCC TCGCTACTCG 
CAGCTGCTGG CAATGCGAGC AACCTCGAAG CATCAGCACC TTAATGGCAA TCAGGTTAAC 
TTGTTGCGCA CACAGATCAC CGCCTATCGG TTGCTGGCGC GGAACAAGCC CATATCCATG 
CAGATGCAGC AGGCGTTGCA GGCCGCACAG CAACAGCCAC CGCCAGGACC TCCAATTGGG 
CCACCTGGAG CACCAGGCGG ACCGCCACCA GGGAGCCAGC ATGCAGGACA ACCGCCCGTG 
CCGCCGCAAC AACAGCAACA ACCGCCACCT TCAGCAGGAA CTCCACCCCA GTGCTCCACG 
CCGCCCGCAA GCAATCCTTA TGGACCGCCA GTTCCTGGCC AGAAAATGCA GGTAGCGCCA 
CCACCGCCAC ACATGCAACA GGGACAGCCA TTGCCACCAC AACCGCCACA AGTGGGTGGA 
CCCCCGCCTA TACAACAGCA GCAACCACCT CAGCAGCAGC AACAACAGTC GCAGCCCCCG 
CCACCGGAGC CACATCAGCA CCAGTTGCCA AACGGAGGCA AACCCCTGAG TATGGGGCCA 
TCTGGCGGGC AACCACTAAT ACCTTCATCA CCCATGCAGC CACAAGTGAG AGGGACACTG 
CCAGGAATGC CGCCAGGTAG TCAGGTTCCG CAACCAGGAG GAGGTCCTCA GCGACAGGTA 
CCACCTGCGG GCATGCCGAT GCCCAAGCCC AATCGCATTA CAACGGTGGC CAAACCCGTC 
GGTCTGGATC CCATAACATT GCTGCAGGAA AGGGAGAATA GGATAGCAGC CAGGATATCA 
TTGCGCATGC AGGAGTTGCA GCGACTGCCG GCCACGATGT CCGAGGATCT GCGTCTGCAA 
GCGGCTATCG AGTTGAGGGC CCTAAGGGTC TTGAACTTCC AGCGACAGCT GCGTATGGAG 
TTTGTTCAGT GCACGAGACG AGATACCACT TTGGAAACAG CGTTAAACAT TAAGCTCTAC 
AAGAGGACCA AGAGGCAGGG CCTGCGGGAG GCACGTGCCA CCGAGAAGCT AGAGAAGCAA 
CAGAAGCTAG AGGCTGAACG AAAGCGTCGC CAAAAGCATC TGGAGTTCTT GGCTGCTGTG 
CTTCAGCACG GCAAGGATCT AAGGGAGTTC CACAGAAATA ATAAGGCCCA GCTTGCAAGG 
ATGAACAAGG CCGTGATGAA TCACCACGCC AATGCCGAGC GTGAACAGAA GAAGGAACAA 
GAGCGCATCG AAAAGGAACG TATGCGACGT CTGATGGCCG AGGATGAGGA GGGTTACCGC 
AAGCTGATTG ATCAAAAGAA GGACAAGAGA TTGGCTTTCC TACTGTCGCA GACAGATGAG 
TATATTAGCA ACCTAACTCA GATGGTGAAA CAGCACAAGG ACGACCAGAT GAAGAAAAAG 
GAGGAGGAGG GCAAGCGGCT GATACAGTTC AAAAAGGAGC TGCTGATGAG CGGAGAGTAC 
ATTGGCATTG ATGAGGGCAG CATTGTTGCC GATATGCGGG TACATGTGGT GGAACAGTGC 
ACGGGTAAGA AACTCACCGG CGATGATGCA CCCATGCTGA AGCACTTGCA CCGCTGGCTG 
AATATGCATC CTGGCTGGGA TTGGATCGAC GACGAGGAGG ACAGCTGTGG GAGCAACGAC 
GATCATAAAC CCAAGGTGGA GGAGCAACCA ACGGCAACAG AAGATGCCAC CGACAAAGCG 
CAGGCGACGG GCAACGATGA GGATGCCAAG GATCTAATTA CCAAGGCCAA GGTGGAGGAC 
GATGAGTACA GGACGGAGGA ACAGACGTAC TACAGCATCG CTCATACCAT TCATGAAAAG 
GTTGTGGAGC AGGCCAGCAT CATGGTTAAT GGTACGCTCA AGGAATACCA GATCAAAGGC 
TTGGAGTGGT TGGTTTCGCT GTACAATAAC AATCTAAATG GTATTTTGGC CGATGAAATG 
GGTTTGGGTA AAACCATTCA AACCATTTCG CTGGTAACCT ACCTTATGGA TCGAAAGAAG 
GTTATGGGTC CTTACTTGAT TATTGTACCA CTCTCCACCC TGCCCAATTG GGTGCTGGAA 
TTCGAGAAGT GGGCGCCAGC CGTGGGTGTA GTCAGCTACA AGGGCAGTCC GCAGGGAAGG 
CGATTGCTGC AAAATCAAAT GAGAGCAACC AAGTTCAATG TGCTGTTAAC CACATATGAG 
TACGTGATTA AGGACAAGGC GGTGCTGGCC AAGATCCAGT GGAAATACAT GATTATCGAC 
GAGGGTCACC GCATGAAGAA CCATCACTGC AAACTCACCC AGGTGCTGAA CACCCACTAC 
ATTGCCCCCT ATCGGTTACT TCTCACGGGT ACACCTCTGC AAAACAAGCT TCCCGAGTTG 
TGGGCGCTAC TTAATTTCCT GCTGCCCTCG ATCTTTAAGT CCTGCTCCAC ATTTGAACAG 
TGGTTCAATG CGCCATTTGC CACCACTGGC GAGAAGGTCG AGCTAAACGA GGAGGAGACC 
ATCCTTATCA TTCGTCGTCT GCACAAGGTT CTTCGTCCTT TCCTGTTGCG TCGCCTCAAA 
AAGGAGGTAG AGCACCAGCT GCCCGACAAG GTCGAGTACA TCATCAAGTG CGACATGTCC 
GCGCTGCAAC GCGTTCTCTA CAAGCACATG CAGAGCAAGG GTGTCCTGCT CACCGATGGT 
TCCGAGAAGG GCAAGCACGG CAAGGGAGGC GCTAAGGCGC TGATGAACAC GATCGTACAG 
CTGCGCAAGC TGTGCAACCA TCCGTTTATG TTCCAGCACA TAGAAGAGAA GTACTGCGAT 
CACACTGGCG GCCACGGAGT TGTATCCGGA CCGGATCTGT ATCGTGTCTC TGGCAAATTC 
GAGCTGCTTG ATCGCATCCT GCCCAAGCTG AAGGCGACCA ATCATCGCGT GCTGCTCTTC 
TGTCAGATGA CCCAATGCAT GACCATCATC GAAGACTACC TGGGATGGCG CCAATTCGGC 
TACCTTCGAC TCGACGGTAC CACGAAGGCC GAGGATCGTG GCGAACTACT GCGAAAATTC 
AACGCCAAAG GCTCGGATGT ATTTGTCTTT TTGCTATCTA CCCGAGCTGG TGGTTTGGGT 
CTTAATCTGC AGACCGCCGA TACTGTAGTA ATCTTCGACT CCGATTGGAA TCCCCATCAG 
GATCTGCAAG CCCAGGATCG TGCCCATCGT ATTGGTCAAC GTAACGAGGT GCGCGTCCTG 
CGACTAATGA CAGTCAACTC GGTGGAAGAG CGCATCTTGG CAGCAGCCAG GTACAAGCTG 
AACATGGACG AGAAGGTCAT CCAGGCTGGT ATGTTCGATC AGAAGTCAAC GGGTAGCGAG 
CGACAGCAGT TCCTACAAAC GATTCTGCAC CAGGATGACA ACGAAGAGGA GGAGGAAAAT 
GAGGTGCCCG ATGACGAAAT GATCAACATG ATGATCGCCC GCAGCGAGGA GGAGATTGAA 
ATATTTAAGC GCATGGATGC TGAGCGCAAG AAGGAGGATG AGGAGATCCA TCCGGGTAGG 
GAACGTCTGA TCGATGAGTC CGAGCTACCC GACTGGTTGA CAAAGGATGA TGACGAAGTA 
GAGCGTTTCC ACTATCAGTA CGACGAGGAT ACCATTCTAG GTCGAGGCTC CCGGCAGCGA 
AAGGAAGTAG ACTACACCGA TAGTTTGACT GAAAAGGAGT GGCTGAAAGC CATCGACGAT 
GGCGCTGAGT TCGACGAGGA AGAGGAGGAA GACGATTCGA AGCGCAAGCG ACGAAAGCGT 
AAGAATCGGA AGGAAGAGTC CGACGATGAT TCGTTGATCC TAAAACGGCG ACGACGCCAA 
AATCTGGACA AGCGGTCCAA GAAGCAGATG CACAAGATTA TGAGTGCGGT TATCAAGCAC 
AACCAAGATG GACGAACCCT GTCCGAACCG TTCATGAAGT TGCCTTCGCG GCAGAGGTTG 
CCCGACTACT ACGAGATCAT TAAGCGGCCA GTTGATATTA AGAAAATCCT GCAGCGCATT 
GAGGACTGCA AGTATGCCGA TCTCAACGAG CTGGAGAAGG ACTTTATGCA GCTATGCCAG 
AATGCACAGA TCTACAACGA AGAGGCGTCT CTGATTTACC TGGACTCGAT TGCCCTGCAA 
AAGGTATTCG TTGGGGCCAG GCAGAGGATA ACAGCTGCAG CGGATGCAGC CGCAGTGGCA 
GCCGGAGATA ACACGGGTGA AGCGCACGGC AATGGAGGTT CGGATAATTC GGACAACGAC 
GACGACGATG GTGGCGATGA CGGCTCCGAC GACGAGGAGA TTGCAACCAC TTCAGCGGCG 
GCTGTCAAAA TGAAGCTGAA ACTGAACAAA TCTCTGGCTA GTGCGCCCGC TACGCCCACG 
CAATCATCGT CGAATGTGAG CAGCGGAGCG GCTACCACAT CCAAGAAGCA GACGCGTCGC 
AAGCGCTCCC AAAAGAAGTA CACTATTTCA GACGATGACG ACGATGACAT GGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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brm cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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