comm cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	comm
Entrez Gene ID	39717
Full Name	commissureless
Synonyms	CG17943,Comm,Dmel\CG17943
General protein information	Preferred Names commissureless Names CG17943 gene product from transcript CG17943-RB CG17943-PA CG17943-PB comm-PA comm-PB commisureless
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 71F2-71F2

mRNA and Protein(s)

mRNA	Protein	Name
NM_079371.4	NP_524095.1	commissureless, isoform A
NM_001274962.1	NP_001261891.1	commissureless, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PRRG4 could re-localize hRobo1 from the cell surface, suggesting that PRRG4 is a functional homologue of Comm. Comm is required for axon guidance and synapse formation in the fly, so PRRG4 could contribute to the autistic symptoms of WAGR by disturbing either of these processes in the developing human brain.
Title: The WAGR syndrome gene PRRG4 is a functional homologue of the commissureless axon guidance gene.

findings show Fra mediates canonical chemoattraction in response to netrin & it functions independently of netrin to activate commissureless transcription, allowing attraction to be coupled to downregulation of repulsion in precrossing commissural axons
Title: A frazzled/DCC-dependent transcriptional switch regulates midline axon guidance.

dNedd4 and ubiquitination are required for Commissureless endocytosis and proper neuromuscular synaptogenesis.
Title: Regulation of Commissureless by the ubiquitin ligase DNedd4 is required for neuromuscular synaptogenesis in Drosophila melanogaster.

These studies provide insights into the specific regulation of dNedd4-mediated ubiquitination of Comm and subsequent internalization of Comm or the Comm/Roundabout complex, critical for CNS and muscle development.
Title: Structural determinants for high-affinity binding in a Nedd4 WW3* domain-Comm PY motif complex.

the ability of Comm to localise intracellularly and hence regulate Robo surface levels requires sequences in both the N-terminal and transmembrane domains
Title: The N-terminal and transmembrane domains of Commissureless are necessary for its function and trafficking within neurons.

The following comm gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the comm cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa18589	NM_001274962.1 Latest version!	Drosophila melanogaster commissureless (comm), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa18589	NM_079371.4 Latest version!	Drosophila melanogaster commissureless (comm), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

comm ( NM_079371.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524095.1 Drosophila melanogaster commissureless (comm), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa18589
Clone ID Related Accession (Same CDS sequence)	NM_079371.4 , NM_001274962.1
Accession Version	NM_079371.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	commissureless, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079371.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATTAGCA CCACGGATTA TCCAACAGTG GAGACCACCA CAACTGCCGA AGAGCTCTAT 
GCGGAGTACA TATCCGCGCC GGCCAGCAGC ATGAGTCCCG CTGCAATTGC CGAGCACCTG 
CAGCAGAATC AGATCACCTT CGAGATACCC AGTGCCCACG ATCTGCGACA CATCGACGCC 
CTCAACTCCT TCAACGCCCT GCTCCAGAGG ATTGGCAACG CAGCGGTGTC CTACGATCCC 
GCTCCACCCA GCGGCTGGTC TCCGGATGGC AGCATCAGCA CCGAGCAGCT CTCCAAGTCG 
GTGGTTCTGG ATCTGGCCGA CTTGCGGGAT AGGTCCGAGG AATCGGGCGA ATCCTCGTGG 
TGGAGCCAGA TCTTCGGGGA TGCCGACATG CATGTGATCA TCAATTATCT GTGGATCGGA 
GTGGTCAGCT CGCTGGTCAT TCTGTCGCTG GTCTTCATCC TCTTCAGCTG CTACTTCTAC 
AGGAAGTTCC GCACTTGGAA AAAATGCAAT AAGGACATAC GTGCCCAGAT CCATGCGGCC 
AGTGACTCGT ACTCCTCGCA CCTGGTTGGT TGCGATGCCA GCAGACTGCT GTTGCACCAG 
CAGATGCAGC ATCCACATCA TCGGAGCAGC GAGGCTGGGT TCTACCAAAT CGAATCGCCG 
CCCTGCTACA CAATCGCCAC CGGATTGCCC AGCTACGATG AGGCACTGCA TCATCAGCCT 
CGGCACTTTG CCTACGGCAT GAAGTTCGTT TATCCCTCGT TGGCGGCCGT GCATCATCAT 
CACCATTGCA TTTCCAATTG GGAGAAGCAG GAGCCGCTGA ATAAGCTGCA AAAGTGCAAG 
CTGTCAGCGG CAGCGGCAGT GGAGGAGGAT AAAGCCGACT CCTCATCGTC CACGTCTGCA 
TCCGCGTCGC CATCCTCTTC GGAATCCAGC AACTTGGCCA CAGCAACGCC TGCCATTTGC 
ATTAACATGC CAAGTGGGCG GCAGGATGAG GAGGTGGATA ATTCAGATTC CGATTCCGCA 
ATTGCAGTTG CAGTGGCAGT AGCACAAAGT TTACAGCCGG CGGCGCCTGC CGACGATGAT 
TGCGCCTCAT TGGTCGTTGT TGTTGCCGCG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524095.1
CDS	227..1339
Translation	MISTTDYPTVETTTTAEELYAEYISAPASSMSPAAIAEHLQQNQITFEIPSAHDLRHIDALNSFNALLQRIGNAAVSYDPAPPSGWSPDGSISTEQLSKSVVLDLADLRDRSEESGESSWWSQIFGDADMHVIINYLWIGVVSSLVILSLVFILFSCYFYRKFRTWKKCNKDIRAQIHAASDSYSSHLVGCDASRLLLHQQMQHPHHRSSEAGFYQIESPPCYTIATGLPSYDEALHHQPRHFAYGMKFVYPSLAAVHHHHHCISNWEKQEPLNKLQKCKLSAAAAVEEDKADSSSSTSASASPSSSESSNLATATPAICINMPSGRQDEEVDNSDSDSAIAVAVAVAQSLQPAAPADDDCASLVVVVAA

Target ORF information:

RefSeq Version	NM_079371.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster commissureless (comm), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079371.4

ORF Insert Sequence:

ATGATTAGCA CCACGGATTA TCCAACAGTG GAGACCACCA CAACTGCCGA AGAGCTCTAT 
GCGGAGTACA TATCCGCGCC GGCCAGCAGC ATGAGTCCCG CTGCAATTGC CGAGCACCTG 
CAGCAGAATC AGATCACCTT CGAGATACCC AGTGCCCACG ATCTGCGACA CATCGACGCC 
CTCAACTCCT TCAACGCCCT GCTCCAGAGG ATTGGCAACG CAGCGGTGTC CTACGATCCC 
GCTCCACCCA GCGGCTGGTC TCCGGATGGC AGCATCAGCA CCGAGCAGCT CTCCAAGTCG 
GTGGTTCTGG ATCTGGCCGA CTTGCGGGAT AGGTCCGAGG AATCGGGCGA ATCCTCGTGG 
TGGAGCCAGA TCTTCGGGGA TGCCGACATG CATGTGATCA TCAATTATCT GTGGATCGGA 
GTGGTCAGCT CGCTGGTCAT TCTGTCGCTG GTCTTCATCC TCTTCAGCTG CTACTTCTAC 
AGGAAGTTCC GCACTTGGAA AAAATGCAAT AAGGACATAC GTGCCCAGAT CCATGCGGCC 
AGTGACTCGT ACTCCTCGCA CCTGGTTGGT TGCGATGCCA GCAGACTGCT GTTGCACCAG 
CAGATGCAGC ATCCACATCA TCGGAGCAGC GAGGCTGGGT TCTACCAAAT CGAATCGCCG 
CCCTGCTACA CAATCGCCAC CGGATTGCCC AGCTACGATG AGGCACTGCA TCATCAGCCT 
CGGCACTTTG CCTACGGCAT GAAGTTCGTT TATCCCTCGT TGGCGGCCGT GCATCATCAT 
CACCATTGCA TTTCCAATTG GGAGAAGCAG GAGCCGCTGA ATAAGCTGCA AAAGTGCAAG 
CTGTCAGCGG CAGCGGCAGT GGAGGAGGAT AAAGCCGACT CCTCATCGTC CACGTCTGCA 
TCCGCGTCGC CATCCTCTTC GGAATCCAGC AACTTGGCCA CAGCAACGCC TGCCATTTGC 
ATTAACATGC CAAGTGGGCG GCAGGATGAG GAGGTGGATA ATTCAGATTC CGATTCCGCA 
ATTGCAGTTG CAGTGGCAGT AGCACAAAGT TTACAGCCGG CGGCGCCTGC CGACGATGAT 
TGCGCCTCAT TGGTCGTTGT TGTTGCCGCG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

comm ( NM_001274962.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261891.1 Drosophila melanogaster commissureless (comm), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa18589
Clone ID Related Accession (Same CDS sequence)	NM_079371.4 , NM_001274962.1
Accession Version	NM_001274962.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	commissureless, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATTAGCA CCACGGATTA TCCAACAGTG GAGACCACCA CAACTGCCGA AGAGCTCTAT 
GCGGAGTACA TATCCGCGCC GGCCAGCAGC ATGAGTCCCG CTGCAATTGC CGAGCACCTG 
CAGCAGAATC AGATCACCTT CGAGATACCC AGTGCCCACG ATCTGCGACA CATCGACGCC 
CTCAACTCCT TCAACGCCCT GCTCCAGAGG ATTGGCAACG CAGCGGTGTC CTACGATCCC 
GCTCCACCCA GCGGCTGGTC TCCGGATGGC AGCATCAGCA CCGAGCAGCT CTCCAAGTCG 
GTGGTTCTGG ATCTGGCCGA CTTGCGGGAT AGGTCCGAGG AATCGGGCGA ATCCTCGTGG 
TGGAGCCAGA TCTTCGGGGA TGCCGACATG CATGTGATCA TCAATTATCT GTGGATCGGA 
GTGGTCAGCT CGCTGGTCAT TCTGTCGCTG GTCTTCATCC TCTTCAGCTG CTACTTCTAC 
AGGAAGTTCC GCACTTGGAA AAAATGCAAT AAGGACATAC GTGCCCAGAT CCATGCGGCC 
AGTGACTCGT ACTCCTCGCA CCTGGTTGGT TGCGATGCCA GCAGACTGCT GTTGCACCAG 
CAGATGCAGC ATCCACATCA TCGGAGCAGC GAGGCTGGGT TCTACCAAAT CGAATCGCCG 
CCCTGCTACA CAATCGCCAC CGGATTGCCC AGCTACGATG AGGCACTGCA TCATCAGCCT 
CGGCACTTTG CCTACGGCAT GAAGTTCGTT TATCCCTCGT TGGCGGCCGT GCATCATCAT 
CACCATTGCA TTTCCAATTG GGAGAAGCAG GAGCCGCTGA ATAAGCTGCA AAAGTGCAAG 
CTGTCAGCGG CAGCGGCAGT GGAGGAGGAT AAAGCCGACT CCTCATCGTC CACGTCTGCA 
TCCGCGTCGC CATCCTCTTC GGAATCCAGC AACTTGGCCA CAGCAACGCC TGCCATTTGC 
ATTAACATGC CAAGTGGGCG GCAGGATGAG GAGGTGGATA ATTCAGATTC CGATTCCGCA 
ATTGCAGTTG CAGTGGCAGT AGCACAAAGT TTACAGCCGG CGGCGCCTGC CGACGATGAT 
TGCGCCTCAT TGGTCGTTGT TGTTGCCGCG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261891.1
CDS	227..1339
Translation	MISTTDYPTVETTTTAEELYAEYISAPASSMSPAAIAEHLQQNQITFEIPSAHDLRHIDALNSFNALLQRIGNAAVSYDPAPPSGWSPDGSISTEQLSKSVVLDLADLRDRSEESGESSWWSQIFGDADMHVIINYLWIGVVSSLVILSLVFILFSCYFYRKFRTWKKCNKDIRAQIHAASDSYSSHLVGCDASRLLLHQQMQHPHHRSSEAGFYQIESPPCYTIATGLPSYDEALHHQPRHFAYGMKFVYPSLAAVHHHHHCISNWEKQEPLNKLQKCKLSAAAAVEEDKADSSSSTSASASPSSSESSNLATATPAICINMPSGRQDEEVDNSDSDSAIAVAVAVAQSLQPAAPADDDCASLVVVVAA

Target ORF information:

RefSeq Version	NM_001274962.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster commissureless (comm), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274962.1

ORF Insert Sequence:

ATGATTAGCA CCACGGATTA TCCAACAGTG GAGACCACCA CAACTGCCGA AGAGCTCTAT 
GCGGAGTACA TATCCGCGCC GGCCAGCAGC ATGAGTCCCG CTGCAATTGC CGAGCACCTG 
CAGCAGAATC AGATCACCTT CGAGATACCC AGTGCCCACG ATCTGCGACA CATCGACGCC 
CTCAACTCCT TCAACGCCCT GCTCCAGAGG ATTGGCAACG CAGCGGTGTC CTACGATCCC 
GCTCCACCCA GCGGCTGGTC TCCGGATGGC AGCATCAGCA CCGAGCAGCT CTCCAAGTCG 
GTGGTTCTGG ATCTGGCCGA CTTGCGGGAT AGGTCCGAGG AATCGGGCGA ATCCTCGTGG 
TGGAGCCAGA TCTTCGGGGA TGCCGACATG CATGTGATCA TCAATTATCT GTGGATCGGA 
GTGGTCAGCT CGCTGGTCAT TCTGTCGCTG GTCTTCATCC TCTTCAGCTG CTACTTCTAC 
AGGAAGTTCC GCACTTGGAA AAAATGCAAT AAGGACATAC GTGCCCAGAT CCATGCGGCC 
AGTGACTCGT ACTCCTCGCA CCTGGTTGGT TGCGATGCCA GCAGACTGCT GTTGCACCAG 
CAGATGCAGC ATCCACATCA TCGGAGCAGC GAGGCTGGGT TCTACCAAAT CGAATCGCCG 
CCCTGCTACA CAATCGCCAC CGGATTGCCC AGCTACGATG AGGCACTGCA TCATCAGCCT 
CGGCACTTTG CCTACGGCAT GAAGTTCGTT TATCCCTCGT TGGCGGCCGT GCATCATCAT 
CACCATTGCA TTTCCAATTG GGAGAAGCAG GAGCCGCTGA ATAAGCTGCA AAAGTGCAAG 
CTGTCAGCGG CAGCGGCAGT GGAGGAGGAT AAAGCCGACT CCTCATCGTC CACGTCTGCA 
TCCGCGTCGC CATCCTCTTC GGAATCCAGC AACTTGGCCA CAGCAACGCC TGCCATTTGC 
ATTAACATGC CAAGTGGGCG GCAGGATGAG GAGGTGGATA ATTCAGATTC CGATTCCGCA 
ATTGCAGTTG CAGTGGCAGT AGCACAAAGT TTACAGCCGG CGGCGCCTGC CGACGATGAT 
TGCGCCTCAT TGGTCGTTGT TGTTGCCGCG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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comm cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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