Toll-6 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Toll-6
Entrez Gene ID	39663
Full Name	CG7250 gene product from transcript CG7250-RC
Synonyms	18w-like,CG7250,CT22359,Dmel\CG7250,TOLL 6,Tl-6,Toll 6,dTLR6,dToll6,toll,toll-6
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 71C2-71C2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001259837.3	NP_001246766.1	Toll-6, isoform C
NM_079357.4	NP_524081.1	Toll-6, isoform A
NM_001259836.2	NP_001246765.1	Toll-6, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Toll-6	NP_001246765.1
Xenopus tropicalis (tropical clawed frog)	LOC100485706	XP_002942581.1
Anopheles gambiae (African malaria mosquito)	TOLL6	XP_320172.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

elevated expression of Pav-KLP underlies transport and plasticity phenotypes in pathway mutants, indicating that Toll-6-FoxO signaling promotes MT dynamics by limiting Pav-KLP expression.
Title: A Toll receptor-FoxO pathway represses Pavarotti/MKLP1 to promote microtubule dynamics in motoneurons.

This study demonistrated that Toll-6 have neurotrophic functions in the Drosophila CNS matching those of DNT1 and DNT2 also pleiotropic functions, maintaining predominantly interneuron survival and regulating motor-axon targeting.
Title: Toll-6 and Toll-7 function as neurotrophin receptors in the Drosophila melanogaster CNS.

The following Toll-6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Toll-6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa18532	NM_079357.4 Latest version!	Drosophila melanogaster Toll-6, transcript variant A (Toll-6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa18532	NM_001259836.2 Latest version!	Drosophila melanogaster Toll-6, transcript variant B (Toll-6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa18532	NM_001259837.3 Latest version!	Drosophila melanogaster Toll-6, transcript variant C (Toll-6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Toll-6 ( NM_001259837.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246766.1 Drosophila melanogaster Toll-6, transcript variant C (Toll-6), mRNA.

Price & Availability↑

CloneID	OFa18532
Clone ID Related Accession (Same CDS sequence)	NM_001259837.3 , NM_079357.4 , NM_001259836.2
Accession Version	NM_001259837.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4545bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Toll-6, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001259837.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATCTACT ATATGCTACT CATACTGCCC GTGGTCCTGG CCCAGGATCA GCAGCACACC 
ACGGAATCGC TGTCCACCAA GCACCACCAG CAGCAGCAGC TGTCGCACTC GAATGCGATA 
ATGGGTGAGG CTGGGGTCTC CAACTCGCAG CTAATGCAGC CCTCGACTCC GGCGCGAACC 
CTGCGACCCT TGACCGCGGG CGCGGGTGGT GACCCTTCGC TGTATGACGC CCCCGACGAC 
TGTCACTTCA TGCCAGCAGC GGGTCTGGAT CAGCCGGAAA TAGCCTTAAC GTGCAACCTG 
CGCACGGTGA ACAGCGAGTT TGACACAACC AATTTCAGTG TTATACCCGC CGAGCACACG 
ATTGCCCTGC ACATACTGTG CAATGACGAA ATCATGGCCA AGAGTCGCCT GGAGGCGCAA 
TCTTTCGCCC ATCTGGTTAG GCTCCAGCAG CTGTCCATCC AGTATTGCAA GCTGGGGCGA 
CTGGGTCGCC AGGTTCTGGA TGGTCTGGAG CAGCTGAGGA ACCTGACGCT CCGGACGCAC 
AACATCCTGT GGCCAGCGCT GAATTTCGAG ATAGAAGCGG ATGCCTTCTC GGTCACTCGG 
AGGTTGGAGA GACTGGACCT CAGTTCGAAC AACATCTGGT CCCTGCCGGA TAACATATTC 
TGCACCCTGT CCGAGTTGTC TGCCCTGAAC ATGAGCGAGA ATCGCCTGCA GGACGTCAAC 
GAGCTGGGCT TCAGGGATCG CAGCAAGGAG CCAACCAATG GTTCCACCGA ATCCACCTCC 
ACCACCGAAT CGGCCAAAAA GAGCAGTAGC TCAAGCACCA GTTGTTCGCT GGACCTGGAG 
TACCTGGATG TGAGCCACAA TGATTTCGTC GTGCTGCCAG CAAATGGATT TGGCACTCTG 
AGGAGGCTGC GAGTCCTTTC GGTCAACAAC AATGGCATCT CCATGATTGC GGACAAGGCT 
CTCAGTGGAC TGAAGAACCT GCAGATCCTG AACCTGAGCT CCAACAAAAT CGTGGCCTTG 
CCCACAGAAC TCTTCGCTGA GCAAGCCAAG ATTATCCAAG AGGTGTACCT GCAGAATAAT 
TCCATAAGTG TGCTGAATCC GCAGCTCTTC TCGAATCTGG ACCAGCTGCA GGCCCTGGAC 
CTGTCCATGA ACCAGATAAC CTCCACCTGG ATCGATAAGA ACACCTTTGT GGGTCTGATT 
CGTCTGGTTC TGCTTAATTT GTCGCACAAC AAGTTGACCA AACTGGAGCC AGAGATCTTC 
AGCGACTTGT ACACCCTGCA GATTCTTAAT CTGCGTCACA ATCAGCTGGA GAACATCGCA 
GCCGACACTT TTGCCCCGAT GAATAATCTG CACACGCTGC TGCTGTCGCA CAACAAACTG 
AAGTATCTGG ATGCATATGC TCTGAACGGA TTGTATGTAC TGTCGCTGCT CTCGCTGGAC 
AATAATGCGC TGATTGGCGT GCATCCGGAT GCCTTCCGGA ACTGCAGTGC CCTGCAGGAC 
CTCAACTTGA ATGGCAATCA GCTGAAGACA GTTCCGCTGG CCCTGAGGAA TATGCGCCAT 
TTGAGAACCG TGGATCTGGG CGAGAACATG ATTACCGTTA TGGAGGATAG TGCGTTTAAA 
GGTCTGGGAA ATCTCTATGG TCTCCGCTTG ATTGGCAACT ACCTGGAGAA TATTACGATG 
CACACCTTCA GGGATCTGCC TAATTTGCAA ATCCTGAATC TCGCCAGAAA CCGCATAGCA 
GTTGTGGAAC CGGGGGCCTT TGAGATGACC TCGAGCATTC AAGCTGTGCG TTTGGATGGC 
AATGAGCTGA ATGACATCAA TGGCCTCTTC AGCAACATGC CCTCCCTTCT GTGGCTGAAT 
ATATCCGATA ATCGTTTGGA ATCCTTTGAT TATGGACATG TGCCATCCAC CCTGCAGTGG 
CTGGACCTCC ATAAGAATCG GTTGAGTTCC TTATCAAATC GTTTTGGTTT GGATAGTGAG 
CTAAAACTAC AAACTCTCGA TGTGAGCTTT AATCAACTTC AGCGCATTGG ACCCAGTTCC 
ATACCAAACT CTATCGAACT GCTGTTCCTG AACGACAACC TGATAACCAC CGTTGATCCA 
GATACCTTTA TGCACAAAAC CAATCTGACC CGGGTGGATC TCTATGCGAA CCAAATCACA 
ACGCTGGACA TCAAGTCCTT GAGGATTCTG CCAGTTTGGG AACATCGAGC TCTGCCAGAG 
TTCTATATTG GTGGAAATCC CTTCACCTGC GATTGTAACA TTGATTGGCT GCAGAAGATT 
AACCATATAA CATCGAGGCA ATATCCAAGG ATTATGGATC TGGAGACCAT ATACTGCAAG 
TTGCTGAACA ACAGGGAGAG GGCCTATATT CCCCTGATCG AAGCTGAACC CAAGCACTTT 
TTGTGCACCT ACAAGACCCA TTGCTTCGCC GTTTGCCATT GCTGTGAGTT CGATGCTTGT 
GATTGCGAGA TGACCTGCCC CACGAATTGC ACGTGCTTCC ATGACCAAAC CTGGTCCACA 
AATATTGTGG AGTGCTCGGG CGCAGCTTAT TCTGAGATGC CGAGACGTGT GCCCATGGAT 
ACCTCTGAGT TGTATATCGA TGGCAACAAC TTTGTTGAAT TGGCGGGACA TTCGTTCCTT 
GGTCGCAAGA ACTTGGCTGT TCTCTATGCG AACAACTCGA ACGTGGCCCA CATATACAAC 
ACCACTTTCA GTGGCCTTAA ACGCCTGTTG ATCCTGCACT TGGAGGATAA TCATATAATC 
AGTCTGGAGG GCAACGAGTT CCACAATCTG GAGAACCTGA GGGAGCTATA TCTGCAATCA 
AACAAAATAG CCAGCATCGC CAATGGGAGT TTCCAGATGC TGAGGAAACT GGAGGTCCTG 
CGATTGGATG GCAATCGATT GATGCACTTC GAGGTGTGGC AACTAAGTGC CAATCCCTAC 
CTGGTGGAGA TCAGCTTGGC CGACAACCAG TGGAGCTGTG AGTGTGGTTA CCTGGCCAGG 
TTCAGAAACT ACCTGGGTCA GAGCTCGGAG AAGATCATAG ATGCCTCCAG GGTGAGCTGC 
ATCTACAACA ATGCTACGAG TGTTCTGAGG GAGAAGAACG GTACCAAGTG CACTTTGCGA 
GATGGTGTAG CCCACTATAT GCACACCAAC GAGATCGAGG GACTGCTGCC CCTGCTCCTG 
GTGGCCACCT GTGCCTTTGT GGCCTTTTTC GGTCTGATTT TCGGACTCTT CTGCTATCGC 
CATGAGCTGA AGATATGGGC CCACTCCACC AACTGTCTAA TGAACTTCTG CTACAAGTCA 
CCCCGATTCG TTGACCAGCT GGACAAAGAG CGTCCCAATG ATGCCTACTT CGCCTACAGC 
CTGCAGGACG AGCACTTCGT CAACCAGATC CTGGCCCAGA CGCTGGAAAA CGACATCGGC 
TATCGACTGT GCCTGCACTA CCGCGATGTA AACATCAATG CCTACATCAC GGATGCCCTC 
ATCGAAGCCG CCGAAAGTGC CAAACAGTTT GTGCTCGTGC TGTCCAAGAA CTTCCTGTAC 
AACGAGTGGA GTCGCTTCGA GTACAAGAGT GCTCTCCACG AGCTGGTGAA GCGCAGGAAG 
CGGGTGGTCT TCATCCTGTA TGGAGATCTG CCCCAGCGCG ACATTGACAT GGACATGCGG 
CACTATCTGC GTACCAGCAC CTGCATCGAG TGGGACGACA AGAAGTTTTG GCAAAAGCTG 
CGCCTGGCCT TGCCCCTGCC AAATGGTCGT GGCAACAACA ACAAGCGAGT GGTATCCGGC 
TGCCTATCGG GACGCACTCC CTCCGTGAAT ATGTACGCCA CAAGTCATGA GTATCAGGCC 
GGCAATGGTG GGGTGATACC GCCACCCAGT GCCCGCTACG CGGACTGTGG CAGCAACAAT 
TACGCGACCA TCAACGAGTG TGCTGCAGCT GGAGGAGGTC GTGGGTACAA GCCCATACCC 
ACTTCAGCCT CGGCGGCGGC AGCAGCTTGT AAATTTAACA CCATGAACCA GCTGTCCAAG 
AAGCAGCAAC GGGATCTCAG TGTGGCCGGT ATGGCCAAGA CCCTGGAGCA TCAGCACCAC 
CACAACCACC AAGCGAATCG CAGGAGCCAG CACGAGTACG CGGTGCCCAG CTATCTGCCC 
AGTGCTGCTC CGGCCTACGA CAGTGTGGAC TACGCCAAGC AGCAGATCCG GAACAATGCC 
AACTGCGAGT GTGTTAACCT GGGCACGGCC AAGAGGGCTG CTGGAAAAAA TCCCGCCTCG 
GGATTGCCAT CCAGCTTTAG CTCGAACTTT GTGCCACCCG GTGGTGCCTC GTACAACTGC 
AAGAAATCCT GCAGTTGCAT TGGAGACGAT GAGCTGCTCT GCAGCTGTGG CGGAGGTGGC 
GGCATTGGGG TCAATCTCCT CGAGAGTGGC ACCCAGAGCA GCGTGACCAT GAGCAGCAGC 
AGCAACAACA GCCGACAGCC GGAACTCACC CACTACGAGT CCAACTTGAG CCTGAACGAC 
GACGAAGACG AGGATCACGA TCAGCAGAAG AACCTGTGGG CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246766.1
CDS	534..5078
Translation	MIYYMLLILPVVLAQDQQHTTESLSTKHHQQQQLSHSNAIMGEAGVSNSQLMQPSTPARTLRPLTAGAGGDPSLYDAPDDCHFMPAAGLDQPEIALTCNLRTVNSEFDTTNFSVIPAEHTIALHILCNDEIMAKSRLEAQSFAHLVRLQQLSIQYCKLGRLGRQVLDGLEQLRNLTLRTHNILWPALNFEIEADAFSVTRRLERLDLSSNNIWSLPDNIFCTLSELSALNMSENRLQDVNELGFRDRSKEPTNGSTESTSTTESAKKSSSSSTSCSLDLEYLDVSHNDFVVLPANGFGTLRRLRVLSVNNNGISMIADKALSGLKNLQILNLSSNKIVALPTELFAEQAKIIQEVYLQNNSISVLNPQLFSNLDQLQALDLSMNQITSTWIDKNTFVGLIRLVLLNLSHNKLTKLEPEIFSDLYTLQILNLRHNQLENIAADTFAPMNNLHTLLLSHNKLKYLDAYALNGLYVLSLLSLDNNALIGVHPDAFRNCSALQDLNLNGNQLKTVPLALRNMRHLRTVDLGENMITVMEDSAFKGLGNLYGLRLIGNYLENITMHTFRDLPNLQILNLARNRIAVVEPGAFEMTSSIQAVRLDGNELNDINGLFSNMPSLLWLNISDNRLESFDYGHVPSTLQWLDLHKNRLSSLSNRFGLDSELKLQTLDVSFNQLQRIGPSSIPNSIELLFLNDNLITTVDPDTFMHKTNLTRVDLYANQITTLDIKSLRILPVWEHRALPEFYIGGNPFTCDCNIDWLQKINHITSRQYPRIMDLETIYCKLLNNRERAYIPLIEAEPKHFLCTYKTHCFAVCHCCEFDACDCEMTCPTNCTCFHDQTWSTNIVECSGAAYSEMPRRVPMDTSELYIDGNNFVELAGHSFLGRKNLAVLYANNSNVAHIYNTTFSGLKRLLILHLEDNHIISLEGNEFHNLENLRELYLQSNKIASIANGSFQMLRKLEVLRLDGNRLMHFEVWQLSANPYLVEISLADNQWSCECGYLARFRNYLGQSSEKIIDASRVSCIYNNATSVLREKNGTKCTLRDGVAHYMHTNEIEGLLPLLLVATCAFVAFFGLIFGLFCYRHELKIWAHSTNCLMNFCYKSPRFVDQLDKERPNDAYFAYSLQDEHFVNQILAQTLENDIGYRLCLHYRDVNINAYITDALIEAAESAKQFVLVLSKNFLYNEWSRFEYKSALHELVKRRKRVVFILYGDLPQRDIDMDMRHYLRTSTCIEWDDKKFWQKLRLALPLPNGRGNNNKRVVSGCLSGRTPSVNMYATSHEYQAGNGGVIPPPSARYADCGSNNYATINECAAAGGGRGYKPIPTSASAAAAACKFNTMNQLSKKQQRDLSVAGMAKTLEHQHHHNHQANRRSQHEYAVPSYLPSAAPAYDSVDYAKQQIRNNANCECVNLGTAKRAAGKNPASGLPSSFSSNFVPPGGASYNCKKSCSCIGDDELLCSCGGGGGIGVNLLESGTQSSVTMSSSSNNSRQPELTHYESNLSLNDDEDEDHDQQKNLWA

Target ORF information:

RefSeq Version	NM_001259837.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Toll-6, transcript variant C (Toll-6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259837.3

ORF Insert Sequence:

ATGATCTACT ATATGCTACT CATACTGCCC GTGGTCCTGG CCCAGGATCA GCAGCACACC 
ACGGAATCGC TGTCCACCAA GCACCACCAG CAGCAGCAGC TGTCGCACTC GAATGCGATA 
ATGGGTGAGG CTGGGGTCTC CAACTCGCAG CTAATGCAGC CCTCGACTCC GGCGCGAACC 
CTGCGACCCT TGACCGCGGG CGCGGGTGGT GACCCTTCGC TGTATGACGC CCCCGACGAC 
TGTCACTTCA TGCCAGCAGC GGGTCTGGAT CAGCCGGAAA TAGCCTTAAC GTGCAACCTG 
CGCACGGTGA ACAGCGAGTT TGACACAACC AATTTCAGTG TTATACCCGC CGAGCACACG 
ATTGCCCTGC ACATACTGTG CAATGACGAA ATCATGGCCA AGAGTCGCCT GGAGGCGCAA 
TCTTTCGCCC ATCTGGTTAG GCTCCAGCAG CTGTCCATCC AGTATTGCAA GCTGGGGCGA 
CTGGGTCGCC AGGTTCTGGA TGGTCTGGAG CAGCTGAGGA ACCTGACGCT CCGGACGCAC 
AACATCCTGT GGCCAGCGCT GAATTTCGAG ATAGAAGCGG ATGCCTTCTC GGTCACTCGG 
AGGTTGGAGA GACTGGACCT CAGTTCGAAC AACATCTGGT CCCTGCCGGA TAACATATTC 
TGCACCCTGT CCGAGTTGTC TGCCCTGAAC ATGAGCGAGA ATCGCCTGCA GGACGTCAAC 
GAGCTGGGCT TCAGGGATCG CAGCAAGGAG CCAACCAATG GTTCCACCGA ATCCACCTCC 
ACCACCGAAT CGGCCAAAAA GAGCAGTAGC TCAAGCACCA GTTGTTCGCT GGACCTGGAG 
TACCTGGATG TGAGCCACAA TGATTTCGTC GTGCTGCCAG CAAATGGATT TGGCACTCTG 
AGGAGGCTGC GAGTCCTTTC GGTCAACAAC AATGGCATCT CCATGATTGC GGACAAGGCT 
CTCAGTGGAC TGAAGAACCT GCAGATCCTG AACCTGAGCT CCAACAAAAT CGTGGCCTTG 
CCCACAGAAC TCTTCGCTGA GCAAGCCAAG ATTATCCAAG AGGTGTACCT GCAGAATAAT 
TCCATAAGTG TGCTGAATCC GCAGCTCTTC TCGAATCTGG ACCAGCTGCA GGCCCTGGAC 
CTGTCCATGA ACCAGATAAC CTCCACCTGG ATCGATAAGA ACACCTTTGT GGGTCTGATT 
CGTCTGGTTC TGCTTAATTT GTCGCACAAC AAGTTGACCA AACTGGAGCC AGAGATCTTC 
AGCGACTTGT ACACCCTGCA GATTCTTAAT CTGCGTCACA ATCAGCTGGA GAACATCGCA 
GCCGACACTT TTGCCCCGAT GAATAATCTG CACACGCTGC TGCTGTCGCA CAACAAACTG 
AAGTATCTGG ATGCATATGC TCTGAACGGA TTGTATGTAC TGTCGCTGCT CTCGCTGGAC 
AATAATGCGC TGATTGGCGT GCATCCGGAT GCCTTCCGGA ACTGCAGTGC CCTGCAGGAC 
CTCAACTTGA ATGGCAATCA GCTGAAGACA GTTCCGCTGG CCCTGAGGAA TATGCGCCAT 
TTGAGAACCG TGGATCTGGG CGAGAACATG ATTACCGTTA TGGAGGATAG TGCGTTTAAA 
GGTCTGGGAA ATCTCTATGG TCTCCGCTTG ATTGGCAACT ACCTGGAGAA TATTACGATG 
CACACCTTCA GGGATCTGCC TAATTTGCAA ATCCTGAATC TCGCCAGAAA CCGCATAGCA 
GTTGTGGAAC CGGGGGCCTT TGAGATGACC TCGAGCATTC AAGCTGTGCG TTTGGATGGC 
AATGAGCTGA ATGACATCAA TGGCCTCTTC AGCAACATGC CCTCCCTTCT GTGGCTGAAT 
ATATCCGATA ATCGTTTGGA ATCCTTTGAT TATGGACATG TGCCATCCAC CCTGCAGTGG 
CTGGACCTCC ATAAGAATCG GTTGAGTTCC TTATCAAATC GTTTTGGTTT GGATAGTGAG 
CTAAAACTAC AAACTCTCGA TGTGAGCTTT AATCAACTTC AGCGCATTGG ACCCAGTTCC 
ATACCAAACT CTATCGAACT GCTGTTCCTG AACGACAACC TGATAACCAC CGTTGATCCA 
GATACCTTTA TGCACAAAAC CAATCTGACC CGGGTGGATC TCTATGCGAA CCAAATCACA 
ACGCTGGACA TCAAGTCCTT GAGGATTCTG CCAGTTTGGG AACATCGAGC TCTGCCAGAG 
TTCTATATTG GTGGAAATCC CTTCACCTGC GATTGTAACA TTGATTGGCT GCAGAAGATT 
AACCATATAA CATCGAGGCA ATATCCAAGG ATTATGGATC TGGAGACCAT ATACTGCAAG 
TTGCTGAACA ACAGGGAGAG GGCCTATATT CCCCTGATCG AAGCTGAACC CAAGCACTTT 
TTGTGCACCT ACAAGACCCA TTGCTTCGCC GTTTGCCATT GCTGTGAGTT CGATGCTTGT 
GATTGCGAGA TGACCTGCCC CACGAATTGC ACGTGCTTCC ATGACCAAAC CTGGTCCACA 
AATATTGTGG AGTGCTCGGG CGCAGCTTAT TCTGAGATGC CGAGACGTGT GCCCATGGAT 
ACCTCTGAGT TGTATATCGA TGGCAACAAC TTTGTTGAAT TGGCGGGACA TTCGTTCCTT 
GGTCGCAAGA ACTTGGCTGT TCTCTATGCG AACAACTCGA ACGTGGCCCA CATATACAAC 
ACCACTTTCA GTGGCCTTAA ACGCCTGTTG ATCCTGCACT TGGAGGATAA TCATATAATC 
AGTCTGGAGG GCAACGAGTT CCACAATCTG GAGAACCTGA GGGAGCTATA TCTGCAATCA 
AACAAAATAG CCAGCATCGC CAATGGGAGT TTCCAGATGC TGAGGAAACT GGAGGTCCTG 
CGATTGGATG GCAATCGATT GATGCACTTC GAGGTGTGGC AACTAAGTGC CAATCCCTAC 
CTGGTGGAGA TCAGCTTGGC CGACAACCAG TGGAGCTGTG AGTGTGGTTA CCTGGCCAGG 
TTCAGAAACT ACCTGGGTCA GAGCTCGGAG AAGATCATAG ATGCCTCCAG GGTGAGCTGC 
ATCTACAACA ATGCTACGAG TGTTCTGAGG GAGAAGAACG GTACCAAGTG CACTTTGCGA 
GATGGTGTAG CCCACTATAT GCACACCAAC GAGATCGAGG GACTGCTGCC CCTGCTCCTG 
GTGGCCACCT GTGCCTTTGT GGCCTTTTTC GGTCTGATTT TCGGACTCTT CTGCTATCGC 
CATGAGCTGA AGATATGGGC CCACTCCACC AACTGTCTAA TGAACTTCTG CTACAAGTCA 
CCCCGATTCG TTGACCAGCT GGACAAAGAG CGTCCCAATG ATGCCTACTT CGCCTACAGC 
CTGCAGGACG AGCACTTCGT CAACCAGATC CTGGCCCAGA CGCTGGAAAA CGACATCGGC 
TATCGACTGT GCCTGCACTA CCGCGATGTA AACATCAATG CCTACATCAC GGATGCCCTC 
ATCGAAGCCG CCGAAAGTGC CAAACAGTTT GTGCTCGTGC TGTCCAAGAA CTTCCTGTAC 
AACGAGTGGA GTCGCTTCGA GTACAAGAGT GCTCTCCACG AGCTGGTGAA GCGCAGGAAG 
CGGGTGGTCT TCATCCTGTA TGGAGATCTG CCCCAGCGCG ACATTGACAT GGACATGCGG 
CACTATCTGC GTACCAGCAC CTGCATCGAG TGGGACGACA AGAAGTTTTG GCAAAAGCTG 
CGCCTGGCCT TGCCCCTGCC AAATGGTCGT GGCAACAACA ACAAGCGAGT GGTATCCGGC 
TGCCTATCGG GACGCACTCC CTCCGTGAAT ATGTACGCCA CAAGTCATGA GTATCAGGCC 
GGCAATGGTG GGGTGATACC GCCACCCAGT GCCCGCTACG CGGACTGTGG CAGCAACAAT 
TACGCGACCA TCAACGAGTG TGCTGCAGCT GGAGGAGGTC GTGGGTACAA GCCCATACCC 
ACTTCAGCCT CGGCGGCGGC AGCAGCTTGT AAATTTAACA CCATGAACCA GCTGTCCAAG 
AAGCAGCAAC GGGATCTCAG TGTGGCCGGT ATGGCCAAGA CCCTGGAGCA TCAGCACCAC 
CACAACCACC AAGCGAATCG CAGGAGCCAG CACGAGTACG CGGTGCCCAG CTATCTGCCC 
AGTGCTGCTC CGGCCTACGA CAGTGTGGAC TACGCCAAGC AGCAGATCCG GAACAATGCC 
AACTGCGAGT GTGTTAACCT GGGCACGGCC AAGAGGGCTG CTGGAAAAAA TCCCGCCTCG 
GGATTGCCAT CCAGCTTTAG CTCGAACTTT GTGCCACCCG GTGGTGCCTC GTACAACTGC 
AAGAAATCCT GCAGTTGCAT TGGAGACGAT GAGCTGCTCT GCAGCTGTGG CGGAGGTGGC 
GGCATTGGGG TCAATCTCCT CGAGAGTGGC ACCCAGAGCA GCGTGACCAT GAGCAGCAGC 
AGCAACAACA GCCGACAGCC GGAACTCACC CACTACGAGT CCAACTTGAG CCTGAACGAC 
GACGAAGACG AGGATCACGA TCAGCAGAAG AACCTGTGGG CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Toll-6 ( NM_079357.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524081.1 Drosophila melanogaster Toll-6, transcript variant A (Toll-6), mRNA.

Price & Availability↑

CloneID	OFa18532
Clone ID Related Accession (Same CDS sequence)	NM_001259837.3 , NM_079357.4 , NM_001259836.2
Accession Version	NM_079357.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4545bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Toll-6, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079357.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATCTACT ATATGCTACT CATACTGCCC GTGGTCCTGG CCCAGGATCA GCAGCACACC 
ACGGAATCGC TGTCCACCAA GCACCACCAG CAGCAGCAGC TGTCGCACTC GAATGCGATA 
ATGGGTGAGG CTGGGGTCTC CAACTCGCAG CTAATGCAGC CCTCGACTCC GGCGCGAACC 
CTGCGACCCT TGACCGCGGG CGCGGGTGGT GACCCTTCGC TGTATGACGC CCCCGACGAC 
TGTCACTTCA TGCCAGCAGC GGGTCTGGAT CAGCCGGAAA TAGCCTTAAC GTGCAACCTG 
CGCACGGTGA ACAGCGAGTT TGACACAACC AATTTCAGTG TTATACCCGC CGAGCACACG 
ATTGCCCTGC ACATACTGTG CAATGACGAA ATCATGGCCA AGAGTCGCCT GGAGGCGCAA 
TCTTTCGCCC ATCTGGTTAG GCTCCAGCAG CTGTCCATCC AGTATTGCAA GCTGGGGCGA 
CTGGGTCGCC AGGTTCTGGA TGGTCTGGAG CAGCTGAGGA ACCTGACGCT CCGGACGCAC 
AACATCCTGT GGCCAGCGCT GAATTTCGAG ATAGAAGCGG ATGCCTTCTC GGTCACTCGG 
AGGTTGGAGA GACTGGACCT CAGTTCGAAC AACATCTGGT CCCTGCCGGA TAACATATTC 
TGCACCCTGT CCGAGTTGTC TGCCCTGAAC ATGAGCGAGA ATCGCCTGCA GGACGTCAAC 
GAGCTGGGCT TCAGGGATCG CAGCAAGGAG CCAACCAATG GTTCCACCGA ATCCACCTCC 
ACCACCGAAT CGGCCAAAAA GAGCAGTAGC TCAAGCACCA GTTGTTCGCT GGACCTGGAG 
TACCTGGATG TGAGCCACAA TGATTTCGTC GTGCTGCCAG CAAATGGATT TGGCACTCTG 
AGGAGGCTGC GAGTCCTTTC GGTCAACAAC AATGGCATCT CCATGATTGC GGACAAGGCT 
CTCAGTGGAC TGAAGAACCT GCAGATCCTG AACCTGAGCT CCAACAAAAT CGTGGCCTTG 
CCCACAGAAC TCTTCGCTGA GCAAGCCAAG ATTATCCAAG AGGTGTACCT GCAGAATAAT 
TCCATAAGTG TGCTGAATCC GCAGCTCTTC TCGAATCTGG ACCAGCTGCA GGCCCTGGAC 
CTGTCCATGA ACCAGATAAC CTCCACCTGG ATCGATAAGA ACACCTTTGT GGGTCTGATT 
CGTCTGGTTC TGCTTAATTT GTCGCACAAC AAGTTGACCA AACTGGAGCC AGAGATCTTC 
AGCGACTTGT ACACCCTGCA GATTCTTAAT CTGCGTCACA ATCAGCTGGA GAACATCGCA 
GCCGACACTT TTGCCCCGAT GAATAATCTG CACACGCTGC TGCTGTCGCA CAACAAACTG 
AAGTATCTGG ATGCATATGC TCTGAACGGA TTGTATGTAC TGTCGCTGCT CTCGCTGGAC 
AATAATGCGC TGATTGGCGT GCATCCGGAT GCCTTCCGGA ACTGCAGTGC CCTGCAGGAC 
CTCAACTTGA ATGGCAATCA GCTGAAGACA GTTCCGCTGG CCCTGAGGAA TATGCGCCAT 
TTGAGAACCG TGGATCTGGG CGAGAACATG ATTACCGTTA TGGAGGATAG TGCGTTTAAA 
GGTCTGGGAA ATCTCTATGG TCTCCGCTTG ATTGGCAACT ACCTGGAGAA TATTACGATG 
CACACCTTCA GGGATCTGCC TAATTTGCAA ATCCTGAATC TCGCCAGAAA CCGCATAGCA 
GTTGTGGAAC CGGGGGCCTT TGAGATGACC TCGAGCATTC AAGCTGTGCG TTTGGATGGC 
AATGAGCTGA ATGACATCAA TGGCCTCTTC AGCAACATGC CCTCCCTTCT GTGGCTGAAT 
ATATCCGATA ATCGTTTGGA ATCCTTTGAT TATGGACATG TGCCATCCAC CCTGCAGTGG 
CTGGACCTCC ATAAGAATCG GTTGAGTTCC TTATCAAATC GTTTTGGTTT GGATAGTGAG 
CTAAAACTAC AAACTCTCGA TGTGAGCTTT AATCAACTTC AGCGCATTGG ACCCAGTTCC 
ATACCAAACT CTATCGAACT GCTGTTCCTG AACGACAACC TGATAACCAC CGTTGATCCA 
GATACCTTTA TGCACAAAAC CAATCTGACC CGGGTGGATC TCTATGCGAA CCAAATCACA 
ACGCTGGACA TCAAGTCCTT GAGGATTCTG CCAGTTTGGG AACATCGAGC TCTGCCAGAG 
TTCTATATTG GTGGAAATCC CTTCACCTGC GATTGTAACA TTGATTGGCT GCAGAAGATT 
AACCATATAA CATCGAGGCA ATATCCAAGG ATTATGGATC TGGAGACCAT ATACTGCAAG 
TTGCTGAACA ACAGGGAGAG GGCCTATATT CCCCTGATCG AAGCTGAACC CAAGCACTTT 
TTGTGCACCT ACAAGACCCA TTGCTTCGCC GTTTGCCATT GCTGTGAGTT CGATGCTTGT 
GATTGCGAGA TGACCTGCCC CACGAATTGC ACGTGCTTCC ATGACCAAAC CTGGTCCACA 
AATATTGTGG AGTGCTCGGG CGCAGCTTAT TCTGAGATGC CGAGACGTGT GCCCATGGAT 
ACCTCTGAGT TGTATATCGA TGGCAACAAC TTTGTTGAAT TGGCGGGACA TTCGTTCCTT 
GGTCGCAAGA ACTTGGCTGT TCTCTATGCG AACAACTCGA ACGTGGCCCA CATATACAAC 
ACCACTTTCA GTGGCCTTAA ACGCCTGTTG ATCCTGCACT TGGAGGATAA TCATATAATC 
AGTCTGGAGG GCAACGAGTT CCACAATCTG GAGAACCTGA GGGAGCTATA TCTGCAATCA 
AACAAAATAG CCAGCATCGC CAATGGGAGT TTCCAGATGC TGAGGAAACT GGAGGTCCTG 
CGATTGGATG GCAATCGATT GATGCACTTC GAGGTGTGGC AACTAAGTGC CAATCCCTAC 
CTGGTGGAGA TCAGCTTGGC CGACAACCAG TGGAGCTGTG AGTGTGGTTA CCTGGCCAGG 
TTCAGAAACT ACCTGGGTCA GAGCTCGGAG AAGATCATAG ATGCCTCCAG GGTGAGCTGC 
ATCTACAACA ATGCTACGAG TGTTCTGAGG GAGAAGAACG GTACCAAGTG CACTTTGCGA 
GATGGTGTAG CCCACTATAT GCACACCAAC GAGATCGAGG GACTGCTGCC CCTGCTCCTG 
GTGGCCACCT GTGCCTTTGT GGCCTTTTTC GGTCTGATTT TCGGACTCTT CTGCTATCGC 
CATGAGCTGA AGATATGGGC CCACTCCACC AACTGTCTAA TGAACTTCTG CTACAAGTCA 
CCCCGATTCG TTGACCAGCT GGACAAAGAG CGTCCCAATG ATGCCTACTT CGCCTACAGC 
CTGCAGGACG AGCACTTCGT CAACCAGATC CTGGCCCAGA CGCTGGAAAA CGACATCGGC 
TATCGACTGT GCCTGCACTA CCGCGATGTA AACATCAATG CCTACATCAC GGATGCCCTC 
ATCGAAGCCG CCGAAAGTGC CAAACAGTTT GTGCTCGTGC TGTCCAAGAA CTTCCTGTAC 
AACGAGTGGA GTCGCTTCGA GTACAAGAGT GCTCTCCACG AGCTGGTGAA GCGCAGGAAG 
CGGGTGGTCT TCATCCTGTA TGGAGATCTG CCCCAGCGCG ACATTGACAT GGACATGCGG 
CACTATCTGC GTACCAGCAC CTGCATCGAG TGGGACGACA AGAAGTTTTG GCAAAAGCTG 
CGCCTGGCCT TGCCCCTGCC AAATGGTCGT GGCAACAACA ACAAGCGAGT GGTATCCGGC 
TGCCTATCGG GACGCACTCC CTCCGTGAAT ATGTACGCCA CAAGTCATGA GTATCAGGCC 
GGCAATGGTG GGGTGATACC GCCACCCAGT GCCCGCTACG CGGACTGTGG CAGCAACAAT 
TACGCGACCA TCAACGAGTG TGCTGCAGCT GGAGGAGGTC GTGGGTACAA GCCCATACCC 
ACTTCAGCCT CGGCGGCGGC AGCAGCTTGT AAATTTAACA CCATGAACCA GCTGTCCAAG 
AAGCAGCAAC GGGATCTCAG TGTGGCCGGT ATGGCCAAGA CCCTGGAGCA TCAGCACCAC 
CACAACCACC AAGCGAATCG CAGGAGCCAG CACGAGTACG CGGTGCCCAG CTATCTGCCC 
AGTGCTGCTC CGGCCTACGA CAGTGTGGAC TACGCCAAGC AGCAGATCCG GAACAATGCC 
AACTGCGAGT GTGTTAACCT GGGCACGGCC AAGAGGGCTG CTGGAAAAAA TCCCGCCTCG 
GGATTGCCAT CCAGCTTTAG CTCGAACTTT GTGCCACCCG GTGGTGCCTC GTACAACTGC 
AAGAAATCCT GCAGTTGCAT TGGAGACGAT GAGCTGCTCT GCAGCTGTGG CGGAGGTGGC 
GGCATTGGGG TCAATCTCCT CGAGAGTGGC ACCCAGAGCA GCGTGACCAT GAGCAGCAGC 
AGCAACAACA GCCGACAGCC GGAACTCACC CACTACGAGT CCAACTTGAG CCTGAACGAC 
GACGAAGACG AGGATCACGA TCAGCAGAAG AACCTGTGGG CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524081.1
CDS	534..5078
Translation	MIYYMLLILPVVLAQDQQHTTESLSTKHHQQQQLSHSNAIMGEAGVSNSQLMQPSTPARTLRPLTAGAGGDPSLYDAPDDCHFMPAAGLDQPEIALTCNLRTVNSEFDTTNFSVIPAEHTIALHILCNDEIMAKSRLEAQSFAHLVRLQQLSIQYCKLGRLGRQVLDGLEQLRNLTLRTHNILWPALNFEIEADAFSVTRRLERLDLSSNNIWSLPDNIFCTLSELSALNMSENRLQDVNELGFRDRSKEPTNGSTESTSTTESAKKSSSSSTSCSLDLEYLDVSHNDFVVLPANGFGTLRRLRVLSVNNNGISMIADKALSGLKNLQILNLSSNKIVALPTELFAEQAKIIQEVYLQNNSISVLNPQLFSNLDQLQALDLSMNQITSTWIDKNTFVGLIRLVLLNLSHNKLTKLEPEIFSDLYTLQILNLRHNQLENIAADTFAPMNNLHTLLLSHNKLKYLDAYALNGLYVLSLLSLDNNALIGVHPDAFRNCSALQDLNLNGNQLKTVPLALRNMRHLRTVDLGENMITVMEDSAFKGLGNLYGLRLIGNYLENITMHTFRDLPNLQILNLARNRIAVVEPGAFEMTSSIQAVRLDGNELNDINGLFSNMPSLLWLNISDNRLESFDYGHVPSTLQWLDLHKNRLSSLSNRFGLDSELKLQTLDVSFNQLQRIGPSSIPNSIELLFLNDNLITTVDPDTFMHKTNLTRVDLYANQITTLDIKSLRILPVWEHRALPEFYIGGNPFTCDCNIDWLQKINHITSRQYPRIMDLETIYCKLLNNRERAYIPLIEAEPKHFLCTYKTHCFAVCHCCEFDACDCEMTCPTNCTCFHDQTWSTNIVECSGAAYSEMPRRVPMDTSELYIDGNNFVELAGHSFLGRKNLAVLYANNSNVAHIYNTTFSGLKRLLILHLEDNHIISLEGNEFHNLENLRELYLQSNKIASIANGSFQMLRKLEVLRLDGNRLMHFEVWQLSANPYLVEISLADNQWSCECGYLARFRNYLGQSSEKIIDASRVSCIYNNATSVLREKNGTKCTLRDGVAHYMHTNEIEGLLPLLLVATCAFVAFFGLIFGLFCYRHELKIWAHSTNCLMNFCYKSPRFVDQLDKERPNDAYFAYSLQDEHFVNQILAQTLENDIGYRLCLHYRDVNINAYITDALIEAAESAKQFVLVLSKNFLYNEWSRFEYKSALHELVKRRKRVVFILYGDLPQRDIDMDMRHYLRTSTCIEWDDKKFWQKLRLALPLPNGRGNNNKRVVSGCLSGRTPSVNMYATSHEYQAGNGGVIPPPSARYADCGSNNYATINECAAAGGGRGYKPIPTSASAAAAACKFNTMNQLSKKQQRDLSVAGMAKTLEHQHHHNHQANRRSQHEYAVPSYLPSAAPAYDSVDYAKQQIRNNANCECVNLGTAKRAAGKNPASGLPSSFSSNFVPPGGASYNCKKSCSCIGDDELLCSCGGGGGIGVNLLESGTQSSVTMSSSSNNSRQPELTHYESNLSLNDDEDEDHDQQKNLWA

Target ORF information:

RefSeq Version	NM_079357.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Toll-6, transcript variant A (Toll-6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079357.4

ORF Insert Sequence:

ATGATCTACT ATATGCTACT CATACTGCCC GTGGTCCTGG CCCAGGATCA GCAGCACACC 
ACGGAATCGC TGTCCACCAA GCACCACCAG CAGCAGCAGC TGTCGCACTC GAATGCGATA 
ATGGGTGAGG CTGGGGTCTC CAACTCGCAG CTAATGCAGC CCTCGACTCC GGCGCGAACC 
CTGCGACCCT TGACCGCGGG CGCGGGTGGT GACCCTTCGC TGTATGACGC CCCCGACGAC 
TGTCACTTCA TGCCAGCAGC GGGTCTGGAT CAGCCGGAAA TAGCCTTAAC GTGCAACCTG 
CGCACGGTGA ACAGCGAGTT TGACACAACC AATTTCAGTG TTATACCCGC CGAGCACACG 
ATTGCCCTGC ACATACTGTG CAATGACGAA ATCATGGCCA AGAGTCGCCT GGAGGCGCAA 
TCTTTCGCCC ATCTGGTTAG GCTCCAGCAG CTGTCCATCC AGTATTGCAA GCTGGGGCGA 
CTGGGTCGCC AGGTTCTGGA TGGTCTGGAG CAGCTGAGGA ACCTGACGCT CCGGACGCAC 
AACATCCTGT GGCCAGCGCT GAATTTCGAG ATAGAAGCGG ATGCCTTCTC GGTCACTCGG 
AGGTTGGAGA GACTGGACCT CAGTTCGAAC AACATCTGGT CCCTGCCGGA TAACATATTC 
TGCACCCTGT CCGAGTTGTC TGCCCTGAAC ATGAGCGAGA ATCGCCTGCA GGACGTCAAC 
GAGCTGGGCT TCAGGGATCG CAGCAAGGAG CCAACCAATG GTTCCACCGA ATCCACCTCC 
ACCACCGAAT CGGCCAAAAA GAGCAGTAGC TCAAGCACCA GTTGTTCGCT GGACCTGGAG 
TACCTGGATG TGAGCCACAA TGATTTCGTC GTGCTGCCAG CAAATGGATT TGGCACTCTG 
AGGAGGCTGC GAGTCCTTTC GGTCAACAAC AATGGCATCT CCATGATTGC GGACAAGGCT 
CTCAGTGGAC TGAAGAACCT GCAGATCCTG AACCTGAGCT CCAACAAAAT CGTGGCCTTG 
CCCACAGAAC TCTTCGCTGA GCAAGCCAAG ATTATCCAAG AGGTGTACCT GCAGAATAAT 
TCCATAAGTG TGCTGAATCC GCAGCTCTTC TCGAATCTGG ACCAGCTGCA GGCCCTGGAC 
CTGTCCATGA ACCAGATAAC CTCCACCTGG ATCGATAAGA ACACCTTTGT GGGTCTGATT 
CGTCTGGTTC TGCTTAATTT GTCGCACAAC AAGTTGACCA AACTGGAGCC AGAGATCTTC 
AGCGACTTGT ACACCCTGCA GATTCTTAAT CTGCGTCACA ATCAGCTGGA GAACATCGCA 
GCCGACACTT TTGCCCCGAT GAATAATCTG CACACGCTGC TGCTGTCGCA CAACAAACTG 
AAGTATCTGG ATGCATATGC TCTGAACGGA TTGTATGTAC TGTCGCTGCT CTCGCTGGAC 
AATAATGCGC TGATTGGCGT GCATCCGGAT GCCTTCCGGA ACTGCAGTGC CCTGCAGGAC 
CTCAACTTGA ATGGCAATCA GCTGAAGACA GTTCCGCTGG CCCTGAGGAA TATGCGCCAT 
TTGAGAACCG TGGATCTGGG CGAGAACATG ATTACCGTTA TGGAGGATAG TGCGTTTAAA 
GGTCTGGGAA ATCTCTATGG TCTCCGCTTG ATTGGCAACT ACCTGGAGAA TATTACGATG 
CACACCTTCA GGGATCTGCC TAATTTGCAA ATCCTGAATC TCGCCAGAAA CCGCATAGCA 
GTTGTGGAAC CGGGGGCCTT TGAGATGACC TCGAGCATTC AAGCTGTGCG TTTGGATGGC 
AATGAGCTGA ATGACATCAA TGGCCTCTTC AGCAACATGC CCTCCCTTCT GTGGCTGAAT 
ATATCCGATA ATCGTTTGGA ATCCTTTGAT TATGGACATG TGCCATCCAC CCTGCAGTGG 
CTGGACCTCC ATAAGAATCG GTTGAGTTCC TTATCAAATC GTTTTGGTTT GGATAGTGAG 
CTAAAACTAC AAACTCTCGA TGTGAGCTTT AATCAACTTC AGCGCATTGG ACCCAGTTCC 
ATACCAAACT CTATCGAACT GCTGTTCCTG AACGACAACC TGATAACCAC CGTTGATCCA 
GATACCTTTA TGCACAAAAC CAATCTGACC CGGGTGGATC TCTATGCGAA CCAAATCACA 
ACGCTGGACA TCAAGTCCTT GAGGATTCTG CCAGTTTGGG AACATCGAGC TCTGCCAGAG 
TTCTATATTG GTGGAAATCC CTTCACCTGC GATTGTAACA TTGATTGGCT GCAGAAGATT 
AACCATATAA CATCGAGGCA ATATCCAAGG ATTATGGATC TGGAGACCAT ATACTGCAAG 
TTGCTGAACA ACAGGGAGAG GGCCTATATT CCCCTGATCG AAGCTGAACC CAAGCACTTT 
TTGTGCACCT ACAAGACCCA TTGCTTCGCC GTTTGCCATT GCTGTGAGTT CGATGCTTGT 
GATTGCGAGA TGACCTGCCC CACGAATTGC ACGTGCTTCC ATGACCAAAC CTGGTCCACA 
AATATTGTGG AGTGCTCGGG CGCAGCTTAT TCTGAGATGC CGAGACGTGT GCCCATGGAT 
ACCTCTGAGT TGTATATCGA TGGCAACAAC TTTGTTGAAT TGGCGGGACA TTCGTTCCTT 
GGTCGCAAGA ACTTGGCTGT TCTCTATGCG AACAACTCGA ACGTGGCCCA CATATACAAC 
ACCACTTTCA GTGGCCTTAA ACGCCTGTTG ATCCTGCACT TGGAGGATAA TCATATAATC 
AGTCTGGAGG GCAACGAGTT CCACAATCTG GAGAACCTGA GGGAGCTATA TCTGCAATCA 
AACAAAATAG CCAGCATCGC CAATGGGAGT TTCCAGATGC TGAGGAAACT GGAGGTCCTG 
CGATTGGATG GCAATCGATT GATGCACTTC GAGGTGTGGC AACTAAGTGC CAATCCCTAC 
CTGGTGGAGA TCAGCTTGGC CGACAACCAG TGGAGCTGTG AGTGTGGTTA CCTGGCCAGG 
TTCAGAAACT ACCTGGGTCA GAGCTCGGAG AAGATCATAG ATGCCTCCAG GGTGAGCTGC 
ATCTACAACA ATGCTACGAG TGTTCTGAGG GAGAAGAACG GTACCAAGTG CACTTTGCGA 
GATGGTGTAG CCCACTATAT GCACACCAAC GAGATCGAGG GACTGCTGCC CCTGCTCCTG 
GTGGCCACCT GTGCCTTTGT GGCCTTTTTC GGTCTGATTT TCGGACTCTT CTGCTATCGC 
CATGAGCTGA AGATATGGGC CCACTCCACC AACTGTCTAA TGAACTTCTG CTACAAGTCA 
CCCCGATTCG TTGACCAGCT GGACAAAGAG CGTCCCAATG ATGCCTACTT CGCCTACAGC 
CTGCAGGACG AGCACTTCGT CAACCAGATC CTGGCCCAGA CGCTGGAAAA CGACATCGGC 
TATCGACTGT GCCTGCACTA CCGCGATGTA AACATCAATG CCTACATCAC GGATGCCCTC 
ATCGAAGCCG CCGAAAGTGC CAAACAGTTT GTGCTCGTGC TGTCCAAGAA CTTCCTGTAC 
AACGAGTGGA GTCGCTTCGA GTACAAGAGT GCTCTCCACG AGCTGGTGAA GCGCAGGAAG 
CGGGTGGTCT TCATCCTGTA TGGAGATCTG CCCCAGCGCG ACATTGACAT GGACATGCGG 
CACTATCTGC GTACCAGCAC CTGCATCGAG TGGGACGACA AGAAGTTTTG GCAAAAGCTG 
CGCCTGGCCT TGCCCCTGCC AAATGGTCGT GGCAACAACA ACAAGCGAGT GGTATCCGGC 
TGCCTATCGG GACGCACTCC CTCCGTGAAT ATGTACGCCA CAAGTCATGA GTATCAGGCC 
GGCAATGGTG GGGTGATACC GCCACCCAGT GCCCGCTACG CGGACTGTGG CAGCAACAAT 
TACGCGACCA TCAACGAGTG TGCTGCAGCT GGAGGAGGTC GTGGGTACAA GCCCATACCC 
ACTTCAGCCT CGGCGGCGGC AGCAGCTTGT AAATTTAACA CCATGAACCA GCTGTCCAAG 
AAGCAGCAAC GGGATCTCAG TGTGGCCGGT ATGGCCAAGA CCCTGGAGCA TCAGCACCAC 
CACAACCACC AAGCGAATCG CAGGAGCCAG CACGAGTACG CGGTGCCCAG CTATCTGCCC 
AGTGCTGCTC CGGCCTACGA CAGTGTGGAC TACGCCAAGC AGCAGATCCG GAACAATGCC 
AACTGCGAGT GTGTTAACCT GGGCACGGCC AAGAGGGCTG CTGGAAAAAA TCCCGCCTCG 
GGATTGCCAT CCAGCTTTAG CTCGAACTTT GTGCCACCCG GTGGTGCCTC GTACAACTGC 
AAGAAATCCT GCAGTTGCAT TGGAGACGAT GAGCTGCTCT GCAGCTGTGG CGGAGGTGGC 
GGCATTGGGG TCAATCTCCT CGAGAGTGGC ACCCAGAGCA GCGTGACCAT GAGCAGCAGC 
AGCAACAACA GCCGACAGCC GGAACTCACC CACTACGAGT CCAACTTGAG CCTGAACGAC 
GACGAAGACG AGGATCACGA TCAGCAGAAG AACCTGTGGG CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Toll-6 ( NM_001259836.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246765.1 Drosophila melanogaster Toll-6, transcript variant B (Toll-6), mRNA.

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CloneID	OFa18532
Clone ID Related Accession (Same CDS sequence)	NM_001259837.3 , NM_079357.4 , NM_001259836.2
Accession Version	NM_001259836.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4545bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Toll-6, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001259836.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATCTACT ATATGCTACT CATACTGCCC GTGGTCCTGG CCCAGGATCA GCAGCACACC 
ACGGAATCGC TGTCCACCAA GCACCACCAG CAGCAGCAGC TGTCGCACTC GAATGCGATA 
ATGGGTGAGG CTGGGGTCTC CAACTCGCAG CTAATGCAGC CCTCGACTCC GGCGCGAACC 
CTGCGACCCT TGACCGCGGG CGCGGGTGGT GACCCTTCGC TGTATGACGC CCCCGACGAC 
TGTCACTTCA TGCCAGCAGC GGGTCTGGAT CAGCCGGAAA TAGCCTTAAC GTGCAACCTG 
CGCACGGTGA ACAGCGAGTT TGACACAACC AATTTCAGTG TTATACCCGC CGAGCACACG 
ATTGCCCTGC ACATACTGTG CAATGACGAA ATCATGGCCA AGAGTCGCCT GGAGGCGCAA 
TCTTTCGCCC ATCTGGTTAG GCTCCAGCAG CTGTCCATCC AGTATTGCAA GCTGGGGCGA 
CTGGGTCGCC AGGTTCTGGA TGGTCTGGAG CAGCTGAGGA ACCTGACGCT CCGGACGCAC 
AACATCCTGT GGCCAGCGCT GAATTTCGAG ATAGAAGCGG ATGCCTTCTC GGTCACTCGG 
AGGTTGGAGA GACTGGACCT CAGTTCGAAC AACATCTGGT CCCTGCCGGA TAACATATTC 
TGCACCCTGT CCGAGTTGTC TGCCCTGAAC ATGAGCGAGA ATCGCCTGCA GGACGTCAAC 
GAGCTGGGCT TCAGGGATCG CAGCAAGGAG CCAACCAATG GTTCCACCGA ATCCACCTCC 
ACCACCGAAT CGGCCAAAAA GAGCAGTAGC TCAAGCACCA GTTGTTCGCT GGACCTGGAG 
TACCTGGATG TGAGCCACAA TGATTTCGTC GTGCTGCCAG CAAATGGATT TGGCACTCTG 
AGGAGGCTGC GAGTCCTTTC GGTCAACAAC AATGGCATCT CCATGATTGC GGACAAGGCT 
CTCAGTGGAC TGAAGAACCT GCAGATCCTG AACCTGAGCT CCAACAAAAT CGTGGCCTTG 
CCCACAGAAC TCTTCGCTGA GCAAGCCAAG ATTATCCAAG AGGTGTACCT GCAGAATAAT 
TCCATAAGTG TGCTGAATCC GCAGCTCTTC TCGAATCTGG ACCAGCTGCA GGCCCTGGAC 
CTGTCCATGA ACCAGATAAC CTCCACCTGG ATCGATAAGA ACACCTTTGT GGGTCTGATT 
CGTCTGGTTC TGCTTAATTT GTCGCACAAC AAGTTGACCA AACTGGAGCC AGAGATCTTC 
AGCGACTTGT ACACCCTGCA GATTCTTAAT CTGCGTCACA ATCAGCTGGA GAACATCGCA 
GCCGACACTT TTGCCCCGAT GAATAATCTG CACACGCTGC TGCTGTCGCA CAACAAACTG 
AAGTATCTGG ATGCATATGC TCTGAACGGA TTGTATGTAC TGTCGCTGCT CTCGCTGGAC 
AATAATGCGC TGATTGGCGT GCATCCGGAT GCCTTCCGGA ACTGCAGTGC CCTGCAGGAC 
CTCAACTTGA ATGGCAATCA GCTGAAGACA GTTCCGCTGG CCCTGAGGAA TATGCGCCAT 
TTGAGAACCG TGGATCTGGG CGAGAACATG ATTACCGTTA TGGAGGATAG TGCGTTTAAA 
GGTCTGGGAA ATCTCTATGG TCTCCGCTTG ATTGGCAACT ACCTGGAGAA TATTACGATG 
CACACCTTCA GGGATCTGCC TAATTTGCAA ATCCTGAATC TCGCCAGAAA CCGCATAGCA 
GTTGTGGAAC CGGGGGCCTT TGAGATGACC TCGAGCATTC AAGCTGTGCG TTTGGATGGC 
AATGAGCTGA ATGACATCAA TGGCCTCTTC AGCAACATGC CCTCCCTTCT GTGGCTGAAT 
ATATCCGATA ATCGTTTGGA ATCCTTTGAT TATGGACATG TGCCATCCAC CCTGCAGTGG 
CTGGACCTCC ATAAGAATCG GTTGAGTTCC TTATCAAATC GTTTTGGTTT GGATAGTGAG 
CTAAAACTAC AAACTCTCGA TGTGAGCTTT AATCAACTTC AGCGCATTGG ACCCAGTTCC 
ATACCAAACT CTATCGAACT GCTGTTCCTG AACGACAACC TGATAACCAC CGTTGATCCA 
GATACCTTTA TGCACAAAAC CAATCTGACC CGGGTGGATC TCTATGCGAA CCAAATCACA 
ACGCTGGACA TCAAGTCCTT GAGGATTCTG CCAGTTTGGG AACATCGAGC TCTGCCAGAG 
TTCTATATTG GTGGAAATCC CTTCACCTGC GATTGTAACA TTGATTGGCT GCAGAAGATT 
AACCATATAA CATCGAGGCA ATATCCAAGG ATTATGGATC TGGAGACCAT ATACTGCAAG 
TTGCTGAACA ACAGGGAGAG GGCCTATATT CCCCTGATCG AAGCTGAACC CAAGCACTTT 
TTGTGCACCT ACAAGACCCA TTGCTTCGCC GTTTGCCATT GCTGTGAGTT CGATGCTTGT 
GATTGCGAGA TGACCTGCCC CACGAATTGC ACGTGCTTCC ATGACCAAAC CTGGTCCACA 
AATATTGTGG AGTGCTCGGG CGCAGCTTAT TCTGAGATGC CGAGACGTGT GCCCATGGAT 
ACCTCTGAGT TGTATATCGA TGGCAACAAC TTTGTTGAAT TGGCGGGACA TTCGTTCCTT 
GGTCGCAAGA ACTTGGCTGT TCTCTATGCG AACAACTCGA ACGTGGCCCA CATATACAAC 
ACCACTTTCA GTGGCCTTAA ACGCCTGTTG ATCCTGCACT TGGAGGATAA TCATATAATC 
AGTCTGGAGG GCAACGAGTT CCACAATCTG GAGAACCTGA GGGAGCTATA TCTGCAATCA 
AACAAAATAG CCAGCATCGC CAATGGGAGT TTCCAGATGC TGAGGAAACT GGAGGTCCTG 
CGATTGGATG GCAATCGATT GATGCACTTC GAGGTGTGGC AACTAAGTGC CAATCCCTAC 
CTGGTGGAGA TCAGCTTGGC CGACAACCAG TGGAGCTGTG AGTGTGGTTA CCTGGCCAGG 
TTCAGAAACT ACCTGGGTCA GAGCTCGGAG AAGATCATAG ATGCCTCCAG GGTGAGCTGC 
ATCTACAACA ATGCTACGAG TGTTCTGAGG GAGAAGAACG GTACCAAGTG CACTTTGCGA 
GATGGTGTAG CCCACTATAT GCACACCAAC GAGATCGAGG GACTGCTGCC CCTGCTCCTG 
GTGGCCACCT GTGCCTTTGT GGCCTTTTTC GGTCTGATTT TCGGACTCTT CTGCTATCGC 
CATGAGCTGA AGATATGGGC CCACTCCACC AACTGTCTAA TGAACTTCTG CTACAAGTCA 
CCCCGATTCG TTGACCAGCT GGACAAAGAG CGTCCCAATG ATGCCTACTT CGCCTACAGC 
CTGCAGGACG AGCACTTCGT CAACCAGATC CTGGCCCAGA CGCTGGAAAA CGACATCGGC 
TATCGACTGT GCCTGCACTA CCGCGATGTA AACATCAATG CCTACATCAC GGATGCCCTC 
ATCGAAGCCG CCGAAAGTGC CAAACAGTTT GTGCTCGTGC TGTCCAAGAA CTTCCTGTAC 
AACGAGTGGA GTCGCTTCGA GTACAAGAGT GCTCTCCACG AGCTGGTGAA GCGCAGGAAG 
CGGGTGGTCT TCATCCTGTA TGGAGATCTG CCCCAGCGCG ACATTGACAT GGACATGCGG 
CACTATCTGC GTACCAGCAC CTGCATCGAG TGGGACGACA AGAAGTTTTG GCAAAAGCTG 
CGCCTGGCCT TGCCCCTGCC AAATGGTCGT GGCAACAACA ACAAGCGAGT GGTATCCGGC 
TGCCTATCGG GACGCACTCC CTCCGTGAAT ATGTACGCCA CAAGTCATGA GTATCAGGCC 
GGCAATGGTG GGGTGATACC GCCACCCAGT GCCCGCTACG CGGACTGTGG CAGCAACAAT 
TACGCGACCA TCAACGAGTG TGCTGCAGCT GGAGGAGGTC GTGGGTACAA GCCCATACCC 
ACTTCAGCCT CGGCGGCGGC AGCAGCTTGT AAATTTAACA CCATGAACCA GCTGTCCAAG 
AAGCAGCAAC GGGATCTCAG TGTGGCCGGT ATGGCCAAGA CCCTGGAGCA TCAGCACCAC 
CACAACCACC AAGCGAATCG CAGGAGCCAG CACGAGTACG CGGTGCCCAG CTATCTGCCC 
AGTGCTGCTC CGGCCTACGA CAGTGTGGAC TACGCCAAGC AGCAGATCCG GAACAATGCC 
AACTGCGAGT GTGTTAACCT GGGCACGGCC AAGAGGGCTG CTGGAAAAAA TCCCGCCTCG 
GGATTGCCAT CCAGCTTTAG CTCGAACTTT GTGCCACCCG GTGGTGCCTC GTACAACTGC 
AAGAAATCCT GCAGTTGCAT TGGAGACGAT GAGCTGCTCT GCAGCTGTGG CGGAGGTGGC 
GGCATTGGGG TCAATCTCCT CGAGAGTGGC ACCCAGAGCA GCGTGACCAT GAGCAGCAGC 
AGCAACAACA GCCGACAGCC GGAACTCACC CACTACGAGT CCAACTTGAG CCTGAACGAC 
GACGAAGACG AGGATCACGA TCAGCAGAAG AACCTGTGGG CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246765.1
CDS	534..5078
Translation	MIYYMLLILPVVLAQDQQHTTESLSTKHHQQQQLSHSNAIMGEAGVSNSQLMQPSTPARTLRPLTAGAGGDPSLYDAPDDCHFMPAAGLDQPEIALTCNLRTVNSEFDTTNFSVIPAEHTIALHILCNDEIMAKSRLEAQSFAHLVRLQQLSIQYCKLGRLGRQVLDGLEQLRNLTLRTHNILWPALNFEIEADAFSVTRRLERLDLSSNNIWSLPDNIFCTLSELSALNMSENRLQDVNELGFRDRSKEPTNGSTESTSTTESAKKSSSSSTSCSLDLEYLDVSHNDFVVLPANGFGTLRRLRVLSVNNNGISMIADKALSGLKNLQILNLSSNKIVALPTELFAEQAKIIQEVYLQNNSISVLNPQLFSNLDQLQALDLSMNQITSTWIDKNTFVGLIRLVLLNLSHNKLTKLEPEIFSDLYTLQILNLRHNQLENIAADTFAPMNNLHTLLLSHNKLKYLDAYALNGLYVLSLLSLDNNALIGVHPDAFRNCSALQDLNLNGNQLKTVPLALRNMRHLRTVDLGENMITVMEDSAFKGLGNLYGLRLIGNYLENITMHTFRDLPNLQILNLARNRIAVVEPGAFEMTSSIQAVRLDGNELNDINGLFSNMPSLLWLNISDNRLESFDYGHVPSTLQWLDLHKNRLSSLSNRFGLDSELKLQTLDVSFNQLQRIGPSSIPNSIELLFLNDNLITTVDPDTFMHKTNLTRVDLYANQITTLDIKSLRILPVWEHRALPEFYIGGNPFTCDCNIDWLQKINHITSRQYPRIMDLETIYCKLLNNRERAYIPLIEAEPKHFLCTYKTHCFAVCHCCEFDACDCEMTCPTNCTCFHDQTWSTNIVECSGAAYSEMPRRVPMDTSELYIDGNNFVELAGHSFLGRKNLAVLYANNSNVAHIYNTTFSGLKRLLILHLEDNHIISLEGNEFHNLENLRELYLQSNKIASIANGSFQMLRKLEVLRLDGNRLMHFEVWQLSANPYLVEISLADNQWSCECGYLARFRNYLGQSSEKIIDASRVSCIYNNATSVLREKNGTKCTLRDGVAHYMHTNEIEGLLPLLLVATCAFVAFFGLIFGLFCYRHELKIWAHSTNCLMNFCYKSPRFVDQLDKERPNDAYFAYSLQDEHFVNQILAQTLENDIGYRLCLHYRDVNINAYITDALIEAAESAKQFVLVLSKNFLYNEWSRFEYKSALHELVKRRKRVVFILYGDLPQRDIDMDMRHYLRTSTCIEWDDKKFWQKLRLALPLPNGRGNNNKRVVSGCLSGRTPSVNMYATSHEYQAGNGGVIPPPSARYADCGSNNYATINECAAAGGGRGYKPIPTSASAAAAACKFNTMNQLSKKQQRDLSVAGMAKTLEHQHHHNHQANRRSQHEYAVPSYLPSAAPAYDSVDYAKQQIRNNANCECVNLGTAKRAAGKNPASGLPSSFSSNFVPPGGASYNCKKSCSCIGDDELLCSCGGGGGIGVNLLESGTQSSVTMSSSSNNSRQPELTHYESNLSLNDDEDEDHDQQKNLWA

Target ORF information:

RefSeq Version	NM_001259836.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Toll-6, transcript variant B (Toll-6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259836.2

ORF Insert Sequence:

ATGATCTACT ATATGCTACT CATACTGCCC GTGGTCCTGG CCCAGGATCA GCAGCACACC 
ACGGAATCGC TGTCCACCAA GCACCACCAG CAGCAGCAGC TGTCGCACTC GAATGCGATA 
ATGGGTGAGG CTGGGGTCTC CAACTCGCAG CTAATGCAGC CCTCGACTCC GGCGCGAACC 
CTGCGACCCT TGACCGCGGG CGCGGGTGGT GACCCTTCGC TGTATGACGC CCCCGACGAC 
TGTCACTTCA TGCCAGCAGC GGGTCTGGAT CAGCCGGAAA TAGCCTTAAC GTGCAACCTG 
CGCACGGTGA ACAGCGAGTT TGACACAACC AATTTCAGTG TTATACCCGC CGAGCACACG 
ATTGCCCTGC ACATACTGTG CAATGACGAA ATCATGGCCA AGAGTCGCCT GGAGGCGCAA 
TCTTTCGCCC ATCTGGTTAG GCTCCAGCAG CTGTCCATCC AGTATTGCAA GCTGGGGCGA 
CTGGGTCGCC AGGTTCTGGA TGGTCTGGAG CAGCTGAGGA ACCTGACGCT CCGGACGCAC 
AACATCCTGT GGCCAGCGCT GAATTTCGAG ATAGAAGCGG ATGCCTTCTC GGTCACTCGG 
AGGTTGGAGA GACTGGACCT CAGTTCGAAC AACATCTGGT CCCTGCCGGA TAACATATTC 
TGCACCCTGT CCGAGTTGTC TGCCCTGAAC ATGAGCGAGA ATCGCCTGCA GGACGTCAAC 
GAGCTGGGCT TCAGGGATCG CAGCAAGGAG CCAACCAATG GTTCCACCGA ATCCACCTCC 
ACCACCGAAT CGGCCAAAAA GAGCAGTAGC TCAAGCACCA GTTGTTCGCT GGACCTGGAG 
TACCTGGATG TGAGCCACAA TGATTTCGTC GTGCTGCCAG CAAATGGATT TGGCACTCTG 
AGGAGGCTGC GAGTCCTTTC GGTCAACAAC AATGGCATCT CCATGATTGC GGACAAGGCT 
CTCAGTGGAC TGAAGAACCT GCAGATCCTG AACCTGAGCT CCAACAAAAT CGTGGCCTTG 
CCCACAGAAC TCTTCGCTGA GCAAGCCAAG ATTATCCAAG AGGTGTACCT GCAGAATAAT 
TCCATAAGTG TGCTGAATCC GCAGCTCTTC TCGAATCTGG ACCAGCTGCA GGCCCTGGAC 
CTGTCCATGA ACCAGATAAC CTCCACCTGG ATCGATAAGA ACACCTTTGT GGGTCTGATT 
CGTCTGGTTC TGCTTAATTT GTCGCACAAC AAGTTGACCA AACTGGAGCC AGAGATCTTC 
AGCGACTTGT ACACCCTGCA GATTCTTAAT CTGCGTCACA ATCAGCTGGA GAACATCGCA 
GCCGACACTT TTGCCCCGAT GAATAATCTG CACACGCTGC TGCTGTCGCA CAACAAACTG 
AAGTATCTGG ATGCATATGC TCTGAACGGA TTGTATGTAC TGTCGCTGCT CTCGCTGGAC 
AATAATGCGC TGATTGGCGT GCATCCGGAT GCCTTCCGGA ACTGCAGTGC CCTGCAGGAC 
CTCAACTTGA ATGGCAATCA GCTGAAGACA GTTCCGCTGG CCCTGAGGAA TATGCGCCAT 
TTGAGAACCG TGGATCTGGG CGAGAACATG ATTACCGTTA TGGAGGATAG TGCGTTTAAA 
GGTCTGGGAA ATCTCTATGG TCTCCGCTTG ATTGGCAACT ACCTGGAGAA TATTACGATG 
CACACCTTCA GGGATCTGCC TAATTTGCAA ATCCTGAATC TCGCCAGAAA CCGCATAGCA 
GTTGTGGAAC CGGGGGCCTT TGAGATGACC TCGAGCATTC AAGCTGTGCG TTTGGATGGC 
AATGAGCTGA ATGACATCAA TGGCCTCTTC AGCAACATGC CCTCCCTTCT GTGGCTGAAT 
ATATCCGATA ATCGTTTGGA ATCCTTTGAT TATGGACATG TGCCATCCAC CCTGCAGTGG 
CTGGACCTCC ATAAGAATCG GTTGAGTTCC TTATCAAATC GTTTTGGTTT GGATAGTGAG 
CTAAAACTAC AAACTCTCGA TGTGAGCTTT AATCAACTTC AGCGCATTGG ACCCAGTTCC 
ATACCAAACT CTATCGAACT GCTGTTCCTG AACGACAACC TGATAACCAC CGTTGATCCA 
GATACCTTTA TGCACAAAAC CAATCTGACC CGGGTGGATC TCTATGCGAA CCAAATCACA 
ACGCTGGACA TCAAGTCCTT GAGGATTCTG CCAGTTTGGG AACATCGAGC TCTGCCAGAG 
TTCTATATTG GTGGAAATCC CTTCACCTGC GATTGTAACA TTGATTGGCT GCAGAAGATT 
AACCATATAA CATCGAGGCA ATATCCAAGG ATTATGGATC TGGAGACCAT ATACTGCAAG 
TTGCTGAACA ACAGGGAGAG GGCCTATATT CCCCTGATCG AAGCTGAACC CAAGCACTTT 
TTGTGCACCT ACAAGACCCA TTGCTTCGCC GTTTGCCATT GCTGTGAGTT CGATGCTTGT 
GATTGCGAGA TGACCTGCCC CACGAATTGC ACGTGCTTCC ATGACCAAAC CTGGTCCACA 
AATATTGTGG AGTGCTCGGG CGCAGCTTAT TCTGAGATGC CGAGACGTGT GCCCATGGAT 
ACCTCTGAGT TGTATATCGA TGGCAACAAC TTTGTTGAAT TGGCGGGACA TTCGTTCCTT 
GGTCGCAAGA ACTTGGCTGT TCTCTATGCG AACAACTCGA ACGTGGCCCA CATATACAAC 
ACCACTTTCA GTGGCCTTAA ACGCCTGTTG ATCCTGCACT TGGAGGATAA TCATATAATC 
AGTCTGGAGG GCAACGAGTT CCACAATCTG GAGAACCTGA GGGAGCTATA TCTGCAATCA 
AACAAAATAG CCAGCATCGC CAATGGGAGT TTCCAGATGC TGAGGAAACT GGAGGTCCTG 
CGATTGGATG GCAATCGATT GATGCACTTC GAGGTGTGGC AACTAAGTGC CAATCCCTAC 
CTGGTGGAGA TCAGCTTGGC CGACAACCAG TGGAGCTGTG AGTGTGGTTA CCTGGCCAGG 
TTCAGAAACT ACCTGGGTCA GAGCTCGGAG AAGATCATAG ATGCCTCCAG GGTGAGCTGC 
ATCTACAACA ATGCTACGAG TGTTCTGAGG GAGAAGAACG GTACCAAGTG CACTTTGCGA 
GATGGTGTAG CCCACTATAT GCACACCAAC GAGATCGAGG GACTGCTGCC CCTGCTCCTG 
GTGGCCACCT GTGCCTTTGT GGCCTTTTTC GGTCTGATTT TCGGACTCTT CTGCTATCGC 
CATGAGCTGA AGATATGGGC CCACTCCACC AACTGTCTAA TGAACTTCTG CTACAAGTCA 
CCCCGATTCG TTGACCAGCT GGACAAAGAG CGTCCCAATG ATGCCTACTT CGCCTACAGC 
CTGCAGGACG AGCACTTCGT CAACCAGATC CTGGCCCAGA CGCTGGAAAA CGACATCGGC 
TATCGACTGT GCCTGCACTA CCGCGATGTA AACATCAATG CCTACATCAC GGATGCCCTC 
ATCGAAGCCG CCGAAAGTGC CAAACAGTTT GTGCTCGTGC TGTCCAAGAA CTTCCTGTAC 
AACGAGTGGA GTCGCTTCGA GTACAAGAGT GCTCTCCACG AGCTGGTGAA GCGCAGGAAG 
CGGGTGGTCT TCATCCTGTA TGGAGATCTG CCCCAGCGCG ACATTGACAT GGACATGCGG 
CACTATCTGC GTACCAGCAC CTGCATCGAG TGGGACGACA AGAAGTTTTG GCAAAAGCTG 
CGCCTGGCCT TGCCCCTGCC AAATGGTCGT GGCAACAACA ACAAGCGAGT GGTATCCGGC 
TGCCTATCGG GACGCACTCC CTCCGTGAAT ATGTACGCCA CAAGTCATGA GTATCAGGCC 
GGCAATGGTG GGGTGATACC GCCACCCAGT GCCCGCTACG CGGACTGTGG CAGCAACAAT 
TACGCGACCA TCAACGAGTG TGCTGCAGCT GGAGGAGGTC GTGGGTACAA GCCCATACCC 
ACTTCAGCCT CGGCGGCGGC AGCAGCTTGT AAATTTAACA CCATGAACCA GCTGTCCAAG 
AAGCAGCAAC GGGATCTCAG TGTGGCCGGT ATGGCCAAGA CCCTGGAGCA TCAGCACCAC 
CACAACCACC AAGCGAATCG CAGGAGCCAG CACGAGTACG CGGTGCCCAG CTATCTGCCC 
AGTGCTGCTC CGGCCTACGA CAGTGTGGAC TACGCCAAGC AGCAGATCCG GAACAATGCC 
AACTGCGAGT GTGTTAACCT GGGCACGGCC AAGAGGGCTG CTGGAAAAAA TCCCGCCTCG 
GGATTGCCAT CCAGCTTTAG CTCGAACTTT GTGCCACCCG GTGGTGCCTC GTACAACTGC 
AAGAAATCCT GCAGTTGCAT TGGAGACGAT GAGCTGCTCT GCAGCTGTGG CGGAGGTGGC 
GGCATTGGGG TCAATCTCCT CGAGAGTGGC ACCCAGAGCA GCGTGACCAT GAGCAGCAGC 
AGCAACAACA GCCGACAGCC GGAACTCACC CACTACGAGT CCAACTTGAG CCTGAACGAC 
GACGAAGACG AGGATCACGA TCAGCAGAAG AACCTGTGGG CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Toll-6 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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