bbg cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	bbg
Entrez Gene ID	39583
Full Name	big bang
Synonyms	BBG,C96,CG42230,CG9587,CG9598,Dmel\CG42230,Dmel_CG9587,Dmel_CG9598
General protein information	Preferred Names big bang Names CG42230 gene product from transcript CG42230-RI C96 CG42230-PC CG42230-PE CG42230-PF CG42230-PG CG42230-PH CG42230-PI CG42230-PJ CG42230-PK bbg-PC bbg-PE bbg-PF bbg-PG bbg-PH bbg-PI bbg-PJ bbg-PK
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 70D7-70E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001144480.3	NP_001137952.1	big bang, isoform H
NM_001144481.2	NP_001137953.1	big bang, isoform J
NM_140447.4	NP_648704.2	big bang, isoform I
NM_001144477.2	NP_001137949.1	big bang, isoform E
NM_001259826.1	NP_001246755.1	big bang, isoform K
NM_001144478.4	NP_001137950.1	big bang, isoform F
NM_140448.3	NP_648705.2	big bang, isoform C
NM_001144479.4	NP_001137951.1	big bang, isoform G

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	bbg	NP_001246755.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011384	XP_317940.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We show that in the adult Drosophila midgut, BBG is present at the level of the septate junctions, on the apical side of the enterocytes. In the absence of BBG, these junctions become loose
Title: big bang gene modulates gut immune tolerance in Drosophila.

The following bbg gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bbg cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa18498	NM_140447.4 Latest version!	Drosophila melanogaster big bang (bbg), transcript variant I, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa18499	NM_001144480.3 Latest version!	Drosophila melanogaster big bang (bbg), transcript variant H, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa18499	NM_001144478.4 Latest version!	Drosophila melanogaster big bang (bbg), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa18499	NM_001144479.4 Latest version!	Drosophila melanogaster big bang (bbg), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa18502	NM_001144477.2 Latest version!	Drosophila melanogaster big bang (bbg), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa18501	NM_001144481.2 Latest version!	Drosophila melanogaster big bang (bbg), transcript variant J, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa18500	NM_140448.3 Latest version!	Drosophila melanogaster big bang (bbg), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OFa18500	NM_001259826.1 Latest version!	Drosophila melanogaster big bang (bbg), transcript variant K, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bbg ( NM_140447.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648704.2 Drosophila melanogaster big bang (bbg), transcript variant I, mRNA.

Price & Availability↑

CloneID	OFa18498
Clone ID Related Accession (Same CDS sequence)	NM_140447.4
Accession Version	NM_140447.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	big bang, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_140447.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTCAGAA TTAATCGCTA CAGGGATCTT GGTACCCTCA GTAACCACCA CAGCAATATA 
AGCAGCATTA GCGGCATTAA CGCCAGTAGC AACATCCCGG CAGCAACTTT AATCAGCAGC 
AGCATTAAGA AGACATCCAC TGAGGCGGCA ACCACAAAAC CTGCTGCACC CCAGATCCCC 
GGCAAAATGG AGCTGCTGAG CAGCAACACA GGAACAACAA CGACAACGAC GACAGCAACG 
CACCACCTGC ATCAGGCAAC CACCACTAAA CAGCTGTTGG TGCAGGATTA CCTCAGCTAC 
GCCTCACCAC CCACATATTC CCGCCTGCCG CCCGATGGTC ATGAATTTCC ACCCAATTTC 
AGCGAACCAC TGATCATGCA CAGCCACCCA CTAAAGGTCA CCACCGAGTT GAGTTACGAG 
ATACAGAAAG GCGGAAAGGA TGAGTCCTCT GCACCACCGC TTCCCAAAAC GGGGCCACCA 
GCCACAGTTC CCAGGAAAGT ATATCGCCAG GATTTGGTCA TCAACGTAGA GCCAGCACCC 
AGTTTAACCA GGGACTATCA GAGATCTCTG AGTGGTGGTA CTCCCCGGAA GCCTAGCGAT 
TGGCGCAAGG ATGAAAAGTC GGAGAAATCA GTGCGCGACA AGATTGCCAT GTTCTCGTCA 
AACAACGAAC TGGATGCCAT ACCGCCAGCT CCGGCAACAG CCCCCATTTC AAGCTCCTTC 
ACCCGGAAGC CCTTGAATAG GAGCAGTGAA AATCTTCTGG ACAGTTGCTC ATCATCCTCG 
GCTCCTTCGC TGAAAACTCG CGCGATGAGC GTGGAAAATT TGAATGATGT TCAGCGGCAA 
TATCAGTTGG CCAAACAGCT GCCCCAGTTG CACGTGGCCG ACTCTATGTA TTCCCTGAAC 
ACAGCCACGC CCACACCGAG CTACGCCTCT TTGCCGAGAA GAAGCCACGG GGGATCTTAT 
TCTTCTGGAG TGGAGAGAAG GATCAGTTTC TCGGGAGAAG GCGGAGATGC GGCCAACCGA 
AAGGCAGCCA TTACAAATAT CCTAGAGCAA AGAAGAAGAA GTTTGTCGAA ACTGCGAGGA 
TTGGTCATAC CAGAGAGACC CCAACTGCTG GAACCCATCC TGGATCTGCC GGAGATCAAA 
AGCCAGGTAA AAGCAGCCAG CGGAGAGGAT AGTACGGATA GCGGATTAGG CGAAAGCCAC 
CGCAGTACGG TGAATAGGAA TTGCCAATTG GGTGCAGGAG GTGCCGCAAA TAACTATCGC 
AGCATTTTAA ATACCAATCA AAGAAGACCC CTGGAGCAAC AGCTCTCTCA ACCACCAGCC 
AAACCACCCA GGACATCGTT GACACCACTT CAGCCGAGAA GCATGATGAT ACCACCACCA 
CCACCTCCTT TAGATCAGGA AAGTGATACG GATTCGGTGT TTTCGCACAC TGCTAGGGTT 
GCCACTCCGC CGGAGAAGTT TGCACTAACC AGAACCCTCA GCTCCGAAAC AAACACTTCG 
ATAGCTAGTT CAAACACCTC TACTCTAACA TCAGGATCTT CTGCCGGATC TCAAGCCAGT 
TGCAGTTCGC TGGGCAGCAC ACCAGCGGTG GATCTAACCA GGCGGGTTCT CAAGAGCCAG 
GTGATCAATG GCGAAGCCGT TGCGCTCTCC AGTCGCAAAA GTATTCTGGC CTCGGCCAAA 
TGTCGCAGTG CCAAGAGTCG AGGTCAAGAG GAGGATAACG ACAGCACCGA TGGTGAAGCC 
TGTTCCCTGG CCAATCGTAG AATGAAGCCG ATTTCCAGCT ATAAGCTCCA GCAGCAACAG 
ATCCAACTGG GCAAACAACT GGTAGTGGAT AAGCTGATCA ATGTGGCCGC TTATGTGGAA 
CTAACCTCGG ATACGGACGA CAGCAGCCGT AGATCTGATA CGCCTGCCAA GATCAGTGCC 
ATGTTCATAG ACGAGGAGCG CAAGGCCAGC TTCAAAGGAG ATCCTAACCA GCAGGCTAAG 
GTCAAAGTGG AGCAGGTCAA ACCTATGGTC CTCCCAATGC TGCTACCATC TGCGAAAAGG 
GAACCGCTGA AGCAACAGAC CACCGCCGAA CTGCGCGAAA AGTTCGAAAG GAGTGCTGCA 
GCCCAAGCTC AAACCCAAAA CCACTCGCCC GTAATCCATA AGGTCACCCA GAAGCCGCAT 
CATGAGCGAT TCTCCTCGCT GGACTCCCTG GCGTCCAGTT CCTCCGGCGT TAGTTCCACC 
ACCCAAAACG TGAGCACCAC CCAGGAAACG GCCACCGAAT TCGGCAGCTT TTCCTCGCTG 
GGTAGCAACC AGAGTTTGAT CACCGCCCAG GATGTCCAGC AGATAGTCGA GGAGGCTGAT 
CCTCCACTAA AGACCCCAGA AGCGTTCATC ATCGTCCTGC AGAGGGAAAA TCCCGAGAGC 
AGCATCGGTA TCACTTTGGC CGGCGGTTCT GATTATGAGG CTAAGGAAAT TACGATCCAT 
AAGATCCTGA GCAACACGCC AGCTGCCAAG GATGGACGTC TAAAGAAGGG TGATCGAATC 
CTTGCCGTCA ATGGAATGAG CATGCGCGGA CTGACGCACC GCGAATCCAT CAGTGTGCTT 
AAGACCCCCC GACCTGAGGT CGTGCTAGTG GTGACCCGCT CCGAGTCGCT GGTGGTGAAG 
GCGCTGACCA AGAAACGATC CTCCCTGGGA TCACTTAGCT CGCTCAATGA GAAACCCACG 
GAGCTGGACT ATGAACGCAA GAGGAACTAC CACAAGGCCT CCCGATCTCT GGACTTGGAT 
CTTGATCTGG TGTCCAATGA GGCTGGTGAA TCGCCAGTGG CTACTACTCC CAGCACGGGA 
TCCGTAAGTC CACCGCAGCC GGCGAGCTTG CACGACGAGG ATGCGGAGGC CACCATCGCA 
GGTATCCGGG CCAGGAGGCA GTTGTCCCGC GGAGATGCCG CCAAGCTTAG CACGAGCGAG 
CTCCTGGAAC GGGCGGCAGA GGCCAGAAAT GCCATCGCAG CTGAGATTCG TGCACAAGCG 
GAGGATGCGG CGGCCAGTGG AGGTGGAGCT CGTTGTGTGG AGATTGTTAA GGACAGCTGT 
GGACTGGGTT TCTCCATCGA AGGAGGCTTT GATTCGCCGC TGGGAAATCG TCCCCTTATT 
GTCAAAAAGG TGTTCATGGG TGGTGCCGCC CAGAAGACCA ACCAGGTGCG CAATGGCGAC 
GAAATCCTTA GCATCAATGG TGCCTCCACG TCGCGGATGA CACGTGTGGA TGCCTGGAAC 
TATATGAAGC AACTGCCGCT GGGACCGGTC AAGATTTGTT TCGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648704.2
CDS	338..3625
Translation	MLRINRYRDLGTLSNHHSNISSISGINASSNIPAATLISSSIKKTSTEAATTKPAAPQIPGKMELLSSNTGTTTTTTTATHHLHQATTTKQLLVQDYLSYASPPTYSRLPPDGHEFPPNFSEPLIMHSHPLKVTTELSYEIQKGGKDESSAPPLPKTGPPATVPRKVYRQDLVINVEPAPSLTRDYQRSLSGGTPRKPSDWRKDEKSEKSVRDKIAMFSSNNELDAIPPAPATAPISSSFTRKPLNRSSENLLDSCSSSSAPSLKTRAMSVENLNDVQRQYQLAKQLPQLHVADSMYSLNTATPTPSYASLPRRSHGGSYSSGVERRISFSGEGGDAANRKAAITNILEQRRRSLSKLRGLVIPERPQLLEPILDLPEIKSQVKAASGEDSTDSGLGESHRSTVNRNCQLGAGGAANNYRSILNTNQRRPLEQQLSQPPAKPPRTSLTPLQPRSMMIPPPPPPLDQESDTDSVFSHTARVATPPEKFALTRTLSSETNTSIASSNTSTLTSGSSAGSQASCSSLGSTPAVDLTRRVLKSQVINGEAVALSSRKSILASAKCRSAKSRGQEEDNDSTDGEACSLANRRMKPISSYKLQQQQIQLGKQLVVDKLINVAAYVELTSDTDDSSRRSDTPAKISAMFIDEERKASFKGDPNQQAKVKVEQVKPMVLPMLLPSAKREPLKQQTTAELREKFERSAAAQAQTQNHSPVIHKVTQKPHHERFSSLDSLASSSSGVSSTTQNVSTTQETATEFGSFSSLGSNQSLITAQDVQQIVEEADPPLKTPEAFIIVLQRENPESSIGITLAGGSDYEAKEITIHKILSNTPAAKDGRLKKGDRILAVNGMSMRGLTHRESISVLKTPRPEVVLVVTRSESLVVKALTKKRSSLGSLSSLNEKPTELDYERKRNYHKASRSLDLDLDLVSNEAGESPVATTPSTGSVSPPQPASLHDEDAEATIAGIRARRQLSRGDAAKLSTSELLERAAEARNAIAAEIRAQAEDAAASGGGARCVEIVKDSCGLGFSIEGGFDSPLGNRPLIVKKVFMGGAAQKTNQVRNGDEILSINGASTSRMTRVDAWNYMKQLPLGPVKICFA

Target ORF information:

RefSeq Version	NM_140447.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster big bang (bbg), transcript variant I, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140447.4

ORF Insert Sequence:

ATGCTCAGAA TTAATCGCTA CAGGGATCTT GGTACCCTCA GTAACCACCA CAGCAATATA 
AGCAGCATTA GCGGCATTAA CGCCAGTAGC AACATCCCGG CAGCAACTTT AATCAGCAGC 
AGCATTAAGA AGACATCCAC TGAGGCGGCA ACCACAAAAC CTGCTGCACC CCAGATCCCC 
GGCAAAATGG AGCTGCTGAG CAGCAACACA GGAACAACAA CGACAACGAC GACAGCAACG 
CACCACCTGC ATCAGGCAAC CACCACTAAA CAGCTGTTGG TGCAGGATTA CCTCAGCTAC 
GCCTCACCAC CCACATATTC CCGCCTGCCG CCCGATGGTC ATGAATTTCC ACCCAATTTC 
AGCGAACCAC TGATCATGCA CAGCCACCCA CTAAAGGTCA CCACCGAGTT GAGTTACGAG 
ATACAGAAAG GCGGAAAGGA TGAGTCCTCT GCACCACCGC TTCCCAAAAC GGGGCCACCA 
GCCACAGTTC CCAGGAAAGT ATATCGCCAG GATTTGGTCA TCAACGTAGA GCCAGCACCC 
AGTTTAACCA GGGACTATCA GAGATCTCTG AGTGGTGGTA CTCCCCGGAA GCCTAGCGAT 
TGGCGCAAGG ATGAAAAGTC GGAGAAATCA GTGCGCGACA AGATTGCCAT GTTCTCGTCA 
AACAACGAAC TGGATGCCAT ACCGCCAGCT CCGGCAACAG CCCCCATTTC AAGCTCCTTC 
ACCCGGAAGC CCTTGAATAG GAGCAGTGAA AATCTTCTGG ACAGTTGCTC ATCATCCTCG 
GCTCCTTCGC TGAAAACTCG CGCGATGAGC GTGGAAAATT TGAATGATGT TCAGCGGCAA 
TATCAGTTGG CCAAACAGCT GCCCCAGTTG CACGTGGCCG ACTCTATGTA TTCCCTGAAC 
ACAGCCACGC CCACACCGAG CTACGCCTCT TTGCCGAGAA GAAGCCACGG GGGATCTTAT 
TCTTCTGGAG TGGAGAGAAG GATCAGTTTC TCGGGAGAAG GCGGAGATGC GGCCAACCGA 
AAGGCAGCCA TTACAAATAT CCTAGAGCAA AGAAGAAGAA GTTTGTCGAA ACTGCGAGGA 
TTGGTCATAC CAGAGAGACC CCAACTGCTG GAACCCATCC TGGATCTGCC GGAGATCAAA 
AGCCAGGTAA AAGCAGCCAG CGGAGAGGAT AGTACGGATA GCGGATTAGG CGAAAGCCAC 
CGCAGTACGG TGAATAGGAA TTGCCAATTG GGTGCAGGAG GTGCCGCAAA TAACTATCGC 
AGCATTTTAA ATACCAATCA AAGAAGACCC CTGGAGCAAC AGCTCTCTCA ACCACCAGCC 
AAACCACCCA GGACATCGTT GACACCACTT CAGCCGAGAA GCATGATGAT ACCACCACCA 
CCACCTCCTT TAGATCAGGA AAGTGATACG GATTCGGTGT TTTCGCACAC TGCTAGGGTT 
GCCACTCCGC CGGAGAAGTT TGCACTAACC AGAACCCTCA GCTCCGAAAC AAACACTTCG 
ATAGCTAGTT CAAACACCTC TACTCTAACA TCAGGATCTT CTGCCGGATC TCAAGCCAGT 
TGCAGTTCGC TGGGCAGCAC ACCAGCGGTG GATCTAACCA GGCGGGTTCT CAAGAGCCAG 
GTGATCAATG GCGAAGCCGT TGCGCTCTCC AGTCGCAAAA GTATTCTGGC CTCGGCCAAA 
TGTCGCAGTG CCAAGAGTCG AGGTCAAGAG GAGGATAACG ACAGCACCGA TGGTGAAGCC 
TGTTCCCTGG CCAATCGTAG AATGAAGCCG ATTTCCAGCT ATAAGCTCCA GCAGCAACAG 
ATCCAACTGG GCAAACAACT GGTAGTGGAT AAGCTGATCA ATGTGGCCGC TTATGTGGAA 
CTAACCTCGG ATACGGACGA CAGCAGCCGT AGATCTGATA CGCCTGCCAA GATCAGTGCC 
ATGTTCATAG ACGAGGAGCG CAAGGCCAGC TTCAAAGGAG ATCCTAACCA GCAGGCTAAG 
GTCAAAGTGG AGCAGGTCAA ACCTATGGTC CTCCCAATGC TGCTACCATC TGCGAAAAGG 
GAACCGCTGA AGCAACAGAC CACCGCCGAA CTGCGCGAAA AGTTCGAAAG GAGTGCTGCA 
GCCCAAGCTC AAACCCAAAA CCACTCGCCC GTAATCCATA AGGTCACCCA GAAGCCGCAT 
CATGAGCGAT TCTCCTCGCT GGACTCCCTG GCGTCCAGTT CCTCCGGCGT TAGTTCCACC 
ACCCAAAACG TGAGCACCAC CCAGGAAACG GCCACCGAAT TCGGCAGCTT TTCCTCGCTG 
GGTAGCAACC AGAGTTTGAT CACCGCCCAG GATGTCCAGC AGATAGTCGA GGAGGCTGAT 
CCTCCACTAA AGACCCCAGA AGCGTTCATC ATCGTCCTGC AGAGGGAAAA TCCCGAGAGC 
AGCATCGGTA TCACTTTGGC CGGCGGTTCT GATTATGAGG CTAAGGAAAT TACGATCCAT 
AAGATCCTGA GCAACACGCC AGCTGCCAAG GATGGACGTC TAAAGAAGGG TGATCGAATC 
CTTGCCGTCA ATGGAATGAG CATGCGCGGA CTGACGCACC GCGAATCCAT CAGTGTGCTT 
AAGACCCCCC GACCTGAGGT CGTGCTAGTG GTGACCCGCT CCGAGTCGCT GGTGGTGAAG 
GCGCTGACCA AGAAACGATC CTCCCTGGGA TCACTTAGCT CGCTCAATGA GAAACCCACG 
GAGCTGGACT ATGAACGCAA GAGGAACTAC CACAAGGCCT CCCGATCTCT GGACTTGGAT 
CTTGATCTGG TGTCCAATGA GGCTGGTGAA TCGCCAGTGG CTACTACTCC CAGCACGGGA 
TCCGTAAGTC CACCGCAGCC GGCGAGCTTG CACGACGAGG ATGCGGAGGC CACCATCGCA 
GGTATCCGGG CCAGGAGGCA GTTGTCCCGC GGAGATGCCG CCAAGCTTAG CACGAGCGAG 
CTCCTGGAAC GGGCGGCAGA GGCCAGAAAT GCCATCGCAG CTGAGATTCG TGCACAAGCG 
GAGGATGCGG CGGCCAGTGG AGGTGGAGCT CGTTGTGTGG AGATTGTTAA GGACAGCTGT 
GGACTGGGTT TCTCCATCGA AGGAGGCTTT GATTCGCCGC TGGGAAATCG TCCCCTTATT 
GTCAAAAAGG TGTTCATGGG TGGTGCCGCC CAGAAGACCA ACCAGGTGCG CAATGGCGAC 
GAAATCCTTA GCATCAATGG TGCCTCCACG TCGCGGATGA CACGTGTGGA TGCCTGGAAC 
TATATGAAGC AACTGCCGCT GGGACCGGTC AAGATTTGTT TCGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bbg ( NM_001144480.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137952.1 Drosophila melanogaster big bang (bbg), transcript variant H, mRNA.

Price & Availability↑

CloneID	OFa18499
Clone ID Related Accession (Same CDS sequence)	NM_001144480.3 , NM_001144478.4 , NM_001144479.4
Accession Version	NM_001144480.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	big bang, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001144480.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGCTGC TGAGCAGCAA CACAGGAACA ACAACGACAA CGACGACAGC AACGCACCAC 
CTGCATCAGG CAACCACCAC TAAACAGCTG TTGGTGCAGG ATTACCTCAG CTACGCCTCA 
CCACCCACAT ATTCCCGCCT GCCGCCCGAT GGTCATGAAT TTCCACCCAA TTTCAGCGAA 
CCACTGATCA TGCACAGCCA CCCACTAAAG GTCACCACCG AGTTGAGTTA CGAGATACAG 
AAAGGCGGAA AGGATGAGTC CTCTGCACCA CCGCTTCCCA AAACGGGGCC ACCAGCCACA 
GTTCCCAGGA AAGTATATCG CCAGGATTTG GTCATCAACG TAGAGCCAGC ACCCAGTTTA 
ACCAGGGACT ATCAGAGATC TCTGAGTGGT GGTACTCCCC GGAAGCCTAG CGATTGGCGC 
AAGGATGAAA AGTCGGAGAA ATCAGTGCGC GACAAGATTG CCATGTTCTC GTCAAACAAC 
GAACTGGATG CCATACCGCC AGCTCCGGCA ACAGCCCCCA TTTCAAGCTC CTTCACCCGG 
AAGCCCTTGA ATAGGAGCAG TGAAAATCTT CTGGACAGTT GCTCATCATC CTCGGCTCCT 
TCGCTGAAAA CTCGCGCGAT GAGCGTGGAA AATTTGAATG ATGTTCAGCG GCAATATCAG 
TTGGCCAAAC AGCTGCCCCA GTTGCACGTG GCCGACTCTA TGTATTCCCT GAACACAGCC 
ACGCCCACAC CGAGCTACGC CTCTTTGCCG AGAAGAAGCC ACGGGGGATC TTATTCTTCT 
GGAGTGGAGA GAAGGATCAG TTTCTCGGGA GAAGGCGGAG ATGCGGCCAA CCGAAAGGCA 
GCCATTACAA ATATCCTAGA GCAAAGAAGA AGAAGTTTGT CGAAACTGCG AGGATTGGTC 
ATACCAGAGA GACCCCAACT GCTGGAACCC ATCCTGGATC TGCCGGAGAT CAAAAGCCAG 
GTAAAAGCAG CCAGCGGAGA GGATAGTACG GATAGCGGAT TAGGCGAAAG CCACCGCAGT 
ACGGTGAATA GGAATTGCCA ATTGGGTGCA GGAGGTGCCG CAAATAACTA TCGCAGCATT 
TTAAATACCA ATCAAAGAAG ACCCCTGGAG CAACAGCTCT CTCAACCACC AGCCAAACCA 
CCCAGGACAT CGTTGACACC ACTTCAGCCG AGAAGCATGA TGATACCACC ACCACCACCT 
CCTTTAGATC AGGAAAGTGA TACGGATTCG GTGTTTTCGC ACACTGCTAG GGTTGCCACT 
CCGCCGGAGA AGTTTGCACT AACCAGAACC CTCAGCTCCG AAACAAACAC TTCGATAGCT 
AGTTCAAACA CCTCTACTCT AACATCAGGA TCTTCTGCCG GATCTCAAGC CAGTTGCAGT 
TCGCTGGGCA GCACACCAGC GGTGGATCTA ACCAGGCGGG TTCTCAAGAG CCAGGTGATC 
AATGGCGAAG CCGTTGCGCT CTCCAGTCGC AAAAGTATTC TGGCCTCGGC CAAATGTCGC 
AGTGCCAAGA GTCGAGGTCA AGAGGAGGAT AACGACAGCA CCGATGGTGA AGCCTGTTCC 
CTGGCCAATC GTAGAATGAA GCCGATTTCC AGCTATAAGC TCCAGCAGCA ACAGATCCAA 
CTGGGCAAAC AACTGGTAGT GGATAAGCTG ATCAATGTGG CCGCTTATGT GGAACTAACC 
TCGGATACGG ACGACAGCAG CCGTAGATCT GATACGCCTG CCAAGATCAG TGCCATGTTC 
ATAGACGAGG AGCGCAAGGC CAGCTTCAAA GGAGATCCTA ACCAGCAGGC TAAGGTCAAA 
GTGGAGCAGG TCAAACCTAT GGTCCTCCCA ATGCTGCTAC CATCTGCGAA AAGGGAACCG 
CTGAAGCAAC AGACCACCGC CGAACTGCGC GAAAAGTTCG AAAGGAGTGC TGCAGCCCAA 
GCTCAAACCC AAAACCACTC GCCCGTAATC CATAAGGTCA CCCAGAAGCC GCATCATGAG 
CGATTCTCCT CGCTGGACTC CCTGGCGTCC AGTTCCTCCG GCGTTAGTTC CACCACCCAA 
AACGTGAGCA CCACCCAGGA AACGGCCACC GAATTCGGCA GCTTTTCCTC GCTGGGTAGC 
AACCAGAGTT TGATCACCGC CCAGGATGTC CAGCAGATAG TCGAGGAGGC TGATCCTCCA 
CTAAAGACCC CAGAAGCGTT CATCATCGTC CTGCAGAGGG AAAATCCCGA GAGCAGCATC 
GGTATCACTT TGGCCGGCGG TTCTGATTAT GAGGCTAAGG AAATTACGAT CCATAAGATC 
CTGAGCAACA CGCCAGCTGC CAAGGATGGA CGTCTAAAGA AGGGTGATCG AATCCTTGCC 
GTCAATGGAA TGAGCATGCG CGGACTGACG CACCGCGAAT CCATCAGTGT GCTTAAGACC 
CCCCGACCTG AGGTCGTGCT AGTGGTGACC CGCTCCGAGT CGCTGGTGGT GAAGGCGCTG 
ACCAAGAAAC GATCCTCCCT GGGATCACTT AGCTCGCTCA ATGAGAAACC CACGGAGCTG 
GACTATGAAC GCAAGAGGAA CTACCACAAG GCCTCCCGAT CTCTGGACTT GGATCTTGAT 
CTGGTGTCCA ATGAGGCTGG TGAATCGCCA GTGGCTACTA CTCCCAGCAC GGGATCCGTA 
AGTCCACCGC AGCCGGCGAG CTTGCACGAC GAGGATGCGG AGGCCACCAT CGCAGGTATC 
CGGGCCAGGA GGCAGTTGTC CCGCGGAGAT GCCGCCAAGC TTAGCACGAG CGAGCTCCTG 
GAACGGGCGG CAGAGGCCAG AAATGCCATC GCAGCTGAGA TTCGTGCACA AGCGGAGGAT 
GCGGCGGCCA GTGGAGGTGG AGCTCGTTGT GTGGAGATTG TTAAGGACAG CTGTGGACTG 
GGTTTCTCCA TCGAAGGAGG CTTTGATTCG CCGCTGGGAA ATCGTCCCCT TATTGTCAAA 
AAGGTGTTCA TGGGTGGTGC CGCCCAGAAG ACCAACCAGG TGCGCAATGG CGACGAAATC 
CTTAGCATCA ATGGTGCCTC CACGTCGCGG ATGACACGTG TGGATGCCTG GAACTATATG 
AAGCAACTGC CGCTGGGACC GGTCAAGATT TGTTTCGCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137952.1
CDS	488..3589
Translation	MELLSSNTGTTTTTTTATHHLHQATTTKQLLVQDYLSYASPPTYSRLPPDGHEFPPNFSEPLIMHSHPLKVTTELSYEIQKGGKDESSAPPLPKTGPPATVPRKVYRQDLVINVEPAPSLTRDYQRSLSGGTPRKPSDWRKDEKSEKSVRDKIAMFSSNNELDAIPPAPATAPISSSFTRKPLNRSSENLLDSCSSSSAPSLKTRAMSVENLNDVQRQYQLAKQLPQLHVADSMYSLNTATPTPSYASLPRRSHGGSYSSGVERRISFSGEGGDAANRKAAITNILEQRRRSLSKLRGLVIPERPQLLEPILDLPEIKSQVKAASGEDSTDSGLGESHRSTVNRNCQLGAGGAANNYRSILNTNQRRPLEQQLSQPPAKPPRTSLTPLQPRSMMIPPPPPPLDQESDTDSVFSHTARVATPPEKFALTRTLSSETNTSIASSNTSTLTSGSSAGSQASCSSLGSTPAVDLTRRVLKSQVINGEAVALSSRKSILASAKCRSAKSRGQEEDNDSTDGEACSLANRRMKPISSYKLQQQQIQLGKQLVVDKLINVAAYVELTSDTDDSSRRSDTPAKISAMFIDEERKASFKGDPNQQAKVKVEQVKPMVLPMLLPSAKREPLKQQTTAELREKFERSAAAQAQTQNHSPVIHKVTQKPHHERFSSLDSLASSSSGVSSTTQNVSTTQETATEFGSFSSLGSNQSLITAQDVQQIVEEADPPLKTPEAFIIVLQRENPESSIGITLAGGSDYEAKEITIHKILSNTPAAKDGRLKKGDRILAVNGMSMRGLTHRESISVLKTPRPEVVLVVTRSESLVVKALTKKRSSLGSLSSLNEKPTELDYERKRNYHKASRSLDLDLDLVSNEAGESPVATTPSTGSVSPPQPASLHDEDAEATIAGIRARRQLSRGDAAKLSTSELLERAAEARNAIAAEIRAQAEDAAASGGGARCVEIVKDSCGLGFSIEGGFDSPLGNRPLIVKKVFMGGAAQKTNQVRNGDEILSINGASTSRMTRVDAWNYMKQLPLGPVKICFA

Target ORF information:

RefSeq Version	NM_001144480.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster big bang (bbg), transcript variant H, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144480.3

ORF Insert Sequence:

ATGGAGCTGC TGAGCAGCAA CACAGGAACA ACAACGACAA CGACGACAGC AACGCACCAC 
CTGCATCAGG CAACCACCAC TAAACAGCTG TTGGTGCAGG ATTACCTCAG CTACGCCTCA 
CCACCCACAT ATTCCCGCCT GCCGCCCGAT GGTCATGAAT TTCCACCCAA TTTCAGCGAA 
CCACTGATCA TGCACAGCCA CCCACTAAAG GTCACCACCG AGTTGAGTTA CGAGATACAG 
AAAGGCGGAA AGGATGAGTC CTCTGCACCA CCGCTTCCCA AAACGGGGCC ACCAGCCACA 
GTTCCCAGGA AAGTATATCG CCAGGATTTG GTCATCAACG TAGAGCCAGC ACCCAGTTTA 
ACCAGGGACT ATCAGAGATC TCTGAGTGGT GGTACTCCCC GGAAGCCTAG CGATTGGCGC 
AAGGATGAAA AGTCGGAGAA ATCAGTGCGC GACAAGATTG CCATGTTCTC GTCAAACAAC 
GAACTGGATG CCATACCGCC AGCTCCGGCA ACAGCCCCCA TTTCAAGCTC CTTCACCCGG 
AAGCCCTTGA ATAGGAGCAG TGAAAATCTT CTGGACAGTT GCTCATCATC CTCGGCTCCT 
TCGCTGAAAA CTCGCGCGAT GAGCGTGGAA AATTTGAATG ATGTTCAGCG GCAATATCAG 
TTGGCCAAAC AGCTGCCCCA GTTGCACGTG GCCGACTCTA TGTATTCCCT GAACACAGCC 
ACGCCCACAC CGAGCTACGC CTCTTTGCCG AGAAGAAGCC ACGGGGGATC TTATTCTTCT 
GGAGTGGAGA GAAGGATCAG TTTCTCGGGA GAAGGCGGAG ATGCGGCCAA CCGAAAGGCA 
GCCATTACAA ATATCCTAGA GCAAAGAAGA AGAAGTTTGT CGAAACTGCG AGGATTGGTC 
ATACCAGAGA GACCCCAACT GCTGGAACCC ATCCTGGATC TGCCGGAGAT CAAAAGCCAG 
GTAAAAGCAG CCAGCGGAGA GGATAGTACG GATAGCGGAT TAGGCGAAAG CCACCGCAGT 
ACGGTGAATA GGAATTGCCA ATTGGGTGCA GGAGGTGCCG CAAATAACTA TCGCAGCATT 
TTAAATACCA ATCAAAGAAG ACCCCTGGAG CAACAGCTCT CTCAACCACC AGCCAAACCA 
CCCAGGACAT CGTTGACACC ACTTCAGCCG AGAAGCATGA TGATACCACC ACCACCACCT 
CCTTTAGATC AGGAAAGTGA TACGGATTCG GTGTTTTCGC ACACTGCTAG GGTTGCCACT 
CCGCCGGAGA AGTTTGCACT AACCAGAACC CTCAGCTCCG AAACAAACAC TTCGATAGCT 
AGTTCAAACA CCTCTACTCT AACATCAGGA TCTTCTGCCG GATCTCAAGC CAGTTGCAGT 
TCGCTGGGCA GCACACCAGC GGTGGATCTA ACCAGGCGGG TTCTCAAGAG CCAGGTGATC 
AATGGCGAAG CCGTTGCGCT CTCCAGTCGC AAAAGTATTC TGGCCTCGGC CAAATGTCGC 
AGTGCCAAGA GTCGAGGTCA AGAGGAGGAT AACGACAGCA CCGATGGTGA AGCCTGTTCC 
CTGGCCAATC GTAGAATGAA GCCGATTTCC AGCTATAAGC TCCAGCAGCA ACAGATCCAA 
CTGGGCAAAC AACTGGTAGT GGATAAGCTG ATCAATGTGG CCGCTTATGT GGAACTAACC 
TCGGATACGG ACGACAGCAG CCGTAGATCT GATACGCCTG CCAAGATCAG TGCCATGTTC 
ATAGACGAGG AGCGCAAGGC CAGCTTCAAA GGAGATCCTA ACCAGCAGGC TAAGGTCAAA 
GTGGAGCAGG TCAAACCTAT GGTCCTCCCA ATGCTGCTAC CATCTGCGAA AAGGGAACCG 
CTGAAGCAAC AGACCACCGC CGAACTGCGC GAAAAGTTCG AAAGGAGTGC TGCAGCCCAA 
GCTCAAACCC AAAACCACTC GCCCGTAATC CATAAGGTCA CCCAGAAGCC GCATCATGAG 
CGATTCTCCT CGCTGGACTC CCTGGCGTCC AGTTCCTCCG GCGTTAGTTC CACCACCCAA 
AACGTGAGCA CCACCCAGGA AACGGCCACC GAATTCGGCA GCTTTTCCTC GCTGGGTAGC 
AACCAGAGTT TGATCACCGC CCAGGATGTC CAGCAGATAG TCGAGGAGGC TGATCCTCCA 
CTAAAGACCC CAGAAGCGTT CATCATCGTC CTGCAGAGGG AAAATCCCGA GAGCAGCATC 
GGTATCACTT TGGCCGGCGG TTCTGATTAT GAGGCTAAGG AAATTACGAT CCATAAGATC 
CTGAGCAACA CGCCAGCTGC CAAGGATGGA CGTCTAAAGA AGGGTGATCG AATCCTTGCC 
GTCAATGGAA TGAGCATGCG CGGACTGACG CACCGCGAAT CCATCAGTGT GCTTAAGACC 
CCCCGACCTG AGGTCGTGCT AGTGGTGACC CGCTCCGAGT CGCTGGTGGT GAAGGCGCTG 
ACCAAGAAAC GATCCTCCCT GGGATCACTT AGCTCGCTCA ATGAGAAACC CACGGAGCTG 
GACTATGAAC GCAAGAGGAA CTACCACAAG GCCTCCCGAT CTCTGGACTT GGATCTTGAT 
CTGGTGTCCA ATGAGGCTGG TGAATCGCCA GTGGCTACTA CTCCCAGCAC GGGATCCGTA 
AGTCCACCGC AGCCGGCGAG CTTGCACGAC GAGGATGCGG AGGCCACCAT CGCAGGTATC 
CGGGCCAGGA GGCAGTTGTC CCGCGGAGAT GCCGCCAAGC TTAGCACGAG CGAGCTCCTG 
GAACGGGCGG CAGAGGCCAG AAATGCCATC GCAGCTGAGA TTCGTGCACA AGCGGAGGAT 
GCGGCGGCCA GTGGAGGTGG AGCTCGTTGT GTGGAGATTG TTAAGGACAG CTGTGGACTG 
GGTTTCTCCA TCGAAGGAGG CTTTGATTCG CCGCTGGGAA ATCGTCCCCT TATTGTCAAA 
AAGGTGTTCA TGGGTGGTGC CGCCCAGAAG ACCAACCAGG TGCGCAATGG CGACGAAATC 
CTTAGCATCA ATGGTGCCTC CACGTCGCGG ATGACACGTG TGGATGCCTG GAACTATATG 
AAGCAACTGC CGCTGGGACC GGTCAAGATT TGTTTCGCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bbg ( NM_001144478.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137950.1 Drosophila melanogaster big bang (bbg), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa18499
Clone ID Related Accession (Same CDS sequence)	NM_001144480.3 , NM_001144478.4 , NM_001144479.4
Accession Version	NM_001144478.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	big bang, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001144478.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGCTGC TGAGCAGCAA CACAGGAACA ACAACGACAA CGACGACAGC AACGCACCAC 
CTGCATCAGG CAACCACCAC TAAACAGCTG TTGGTGCAGG ATTACCTCAG CTACGCCTCA 
CCACCCACAT ATTCCCGCCT GCCGCCCGAT GGTCATGAAT TTCCACCCAA TTTCAGCGAA 
CCACTGATCA TGCACAGCCA CCCACTAAAG GTCACCACCG AGTTGAGTTA CGAGATACAG 
AAAGGCGGAA AGGATGAGTC CTCTGCACCA CCGCTTCCCA AAACGGGGCC ACCAGCCACA 
GTTCCCAGGA AAGTATATCG CCAGGATTTG GTCATCAACG TAGAGCCAGC ACCCAGTTTA 
ACCAGGGACT ATCAGAGATC TCTGAGTGGT GGTACTCCCC GGAAGCCTAG CGATTGGCGC 
AAGGATGAAA AGTCGGAGAA ATCAGTGCGC GACAAGATTG CCATGTTCTC GTCAAACAAC 
GAACTGGATG CCATACCGCC AGCTCCGGCA ACAGCCCCCA TTTCAAGCTC CTTCACCCGG 
AAGCCCTTGA ATAGGAGCAG TGAAAATCTT CTGGACAGTT GCTCATCATC CTCGGCTCCT 
TCGCTGAAAA CTCGCGCGAT GAGCGTGGAA AATTTGAATG ATGTTCAGCG GCAATATCAG 
TTGGCCAAAC AGCTGCCCCA GTTGCACGTG GCCGACTCTA TGTATTCCCT GAACACAGCC 
ACGCCCACAC CGAGCTACGC CTCTTTGCCG AGAAGAAGCC ACGGGGGATC TTATTCTTCT 
GGAGTGGAGA GAAGGATCAG TTTCTCGGGA GAAGGCGGAG ATGCGGCCAA CCGAAAGGCA 
GCCATTACAA ATATCCTAGA GCAAAGAAGA AGAAGTTTGT CGAAACTGCG AGGATTGGTC 
ATACCAGAGA GACCCCAACT GCTGGAACCC ATCCTGGATC TGCCGGAGAT CAAAAGCCAG 
GTAAAAGCAG CCAGCGGAGA GGATAGTACG GATAGCGGAT TAGGCGAAAG CCACCGCAGT 
ACGGTGAATA GGAATTGCCA ATTGGGTGCA GGAGGTGCCG CAAATAACTA TCGCAGCATT 
TTAAATACCA ATCAAAGAAG ACCCCTGGAG CAACAGCTCT CTCAACCACC AGCCAAACCA 
CCCAGGACAT CGTTGACACC ACTTCAGCCG AGAAGCATGA TGATACCACC ACCACCACCT 
CCTTTAGATC AGGAAAGTGA TACGGATTCG GTGTTTTCGC ACACTGCTAG GGTTGCCACT 
CCGCCGGAGA AGTTTGCACT AACCAGAACC CTCAGCTCCG AAACAAACAC TTCGATAGCT 
AGTTCAAACA CCTCTACTCT AACATCAGGA TCTTCTGCCG GATCTCAAGC CAGTTGCAGT 
TCGCTGGGCA GCACACCAGC GGTGGATCTA ACCAGGCGGG TTCTCAAGAG CCAGGTGATC 
AATGGCGAAG CCGTTGCGCT CTCCAGTCGC AAAAGTATTC TGGCCTCGGC CAAATGTCGC 
AGTGCCAAGA GTCGAGGTCA AGAGGAGGAT AACGACAGCA CCGATGGTGA AGCCTGTTCC 
CTGGCCAATC GTAGAATGAA GCCGATTTCC AGCTATAAGC TCCAGCAGCA ACAGATCCAA 
CTGGGCAAAC AACTGGTAGT GGATAAGCTG ATCAATGTGG CCGCTTATGT GGAACTAACC 
TCGGATACGG ACGACAGCAG CCGTAGATCT GATACGCCTG CCAAGATCAG TGCCATGTTC 
ATAGACGAGG AGCGCAAGGC CAGCTTCAAA GGAGATCCTA ACCAGCAGGC TAAGGTCAAA 
GTGGAGCAGG TCAAACCTAT GGTCCTCCCA ATGCTGCTAC CATCTGCGAA AAGGGAACCG 
CTGAAGCAAC AGACCACCGC CGAACTGCGC GAAAAGTTCG AAAGGAGTGC TGCAGCCCAA 
GCTCAAACCC AAAACCACTC GCCCGTAATC CATAAGGTCA CCCAGAAGCC GCATCATGAG 
CGATTCTCCT CGCTGGACTC CCTGGCGTCC AGTTCCTCCG GCGTTAGTTC CACCACCCAA 
AACGTGAGCA CCACCCAGGA AACGGCCACC GAATTCGGCA GCTTTTCCTC GCTGGGTAGC 
AACCAGAGTT TGATCACCGC CCAGGATGTC CAGCAGATAG TCGAGGAGGC TGATCCTCCA 
CTAAAGACCC CAGAAGCGTT CATCATCGTC CTGCAGAGGG AAAATCCCGA GAGCAGCATC 
GGTATCACTT TGGCCGGCGG TTCTGATTAT GAGGCTAAGG AAATTACGAT CCATAAGATC 
CTGAGCAACA CGCCAGCTGC CAAGGATGGA CGTCTAAAGA AGGGTGATCG AATCCTTGCC 
GTCAATGGAA TGAGCATGCG CGGACTGACG CACCGCGAAT CCATCAGTGT GCTTAAGACC 
CCCCGACCTG AGGTCGTGCT AGTGGTGACC CGCTCCGAGT CGCTGGTGGT GAAGGCGCTG 
ACCAAGAAAC GATCCTCCCT GGGATCACTT AGCTCGCTCA ATGAGAAACC CACGGAGCTG 
GACTATGAAC GCAAGAGGAA CTACCACAAG GCCTCCCGAT CTCTGGACTT GGATCTTGAT 
CTGGTGTCCA ATGAGGCTGG TGAATCGCCA GTGGCTACTA CTCCCAGCAC GGGATCCGTA 
AGTCCACCGC AGCCGGCGAG CTTGCACGAC GAGGATGCGG AGGCCACCAT CGCAGGTATC 
CGGGCCAGGA GGCAGTTGTC CCGCGGAGAT GCCGCCAAGC TTAGCACGAG CGAGCTCCTG 
GAACGGGCGG CAGAGGCCAG AAATGCCATC GCAGCTGAGA TTCGTGCACA AGCGGAGGAT 
GCGGCGGCCA GTGGAGGTGG AGCTCGTTGT GTGGAGATTG TTAAGGACAG CTGTGGACTG 
GGTTTCTCCA TCGAAGGAGG CTTTGATTCG CCGCTGGGAA ATCGTCCCCT TATTGTCAAA 
AAGGTGTTCA TGGGTGGTGC CGCCCAGAAG ACCAACCAGG TGCGCAATGG CGACGAAATC 
CTTAGCATCA ATGGTGCCTC CACGTCGCGG ATGACACGTG TGGATGCCTG GAACTATATG 
AAGCAACTGC CGCTGGGACC GGTCAAGATT TGTTTCGCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137950.1
CDS	496..3597
Translation	MELLSSNTGTTTTTTTATHHLHQATTTKQLLVQDYLSYASPPTYSRLPPDGHEFPPNFSEPLIMHSHPLKVTTELSYEIQKGGKDESSAPPLPKTGPPATVPRKVYRQDLVINVEPAPSLTRDYQRSLSGGTPRKPSDWRKDEKSEKSVRDKIAMFSSNNELDAIPPAPATAPISSSFTRKPLNRSSENLLDSCSSSSAPSLKTRAMSVENLNDVQRQYQLAKQLPQLHVADSMYSLNTATPTPSYASLPRRSHGGSYSSGVERRISFSGEGGDAANRKAAITNILEQRRRSLSKLRGLVIPERPQLLEPILDLPEIKSQVKAASGEDSTDSGLGESHRSTVNRNCQLGAGGAANNYRSILNTNQRRPLEQQLSQPPAKPPRTSLTPLQPRSMMIPPPPPPLDQESDTDSVFSHTARVATPPEKFALTRTLSSETNTSIASSNTSTLTSGSSAGSQASCSSLGSTPAVDLTRRVLKSQVINGEAVALSSRKSILASAKCRSAKSRGQEEDNDSTDGEACSLANRRMKPISSYKLQQQQIQLGKQLVVDKLINVAAYVELTSDTDDSSRRSDTPAKISAMFIDEERKASFKGDPNQQAKVKVEQVKPMVLPMLLPSAKREPLKQQTTAELREKFERSAAAQAQTQNHSPVIHKVTQKPHHERFSSLDSLASSSSGVSSTTQNVSTTQETATEFGSFSSLGSNQSLITAQDVQQIVEEADPPLKTPEAFIIVLQRENPESSIGITLAGGSDYEAKEITIHKILSNTPAAKDGRLKKGDRILAVNGMSMRGLTHRESISVLKTPRPEVVLVVTRSESLVVKALTKKRSSLGSLSSLNEKPTELDYERKRNYHKASRSLDLDLDLVSNEAGESPVATTPSTGSVSPPQPASLHDEDAEATIAGIRARRQLSRGDAAKLSTSELLERAAEARNAIAAEIRAQAEDAAASGGGARCVEIVKDSCGLGFSIEGGFDSPLGNRPLIVKKVFMGGAAQKTNQVRNGDEILSINGASTSRMTRVDAWNYMKQLPLGPVKICFA

Target ORF information:

RefSeq Version	NM_001144478.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster big bang (bbg), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144478.4

ORF Insert Sequence:

ATGGAGCTGC TGAGCAGCAA CACAGGAACA ACAACGACAA CGACGACAGC AACGCACCAC 
CTGCATCAGG CAACCACCAC TAAACAGCTG TTGGTGCAGG ATTACCTCAG CTACGCCTCA 
CCACCCACAT ATTCCCGCCT GCCGCCCGAT GGTCATGAAT TTCCACCCAA TTTCAGCGAA 
CCACTGATCA TGCACAGCCA CCCACTAAAG GTCACCACCG AGTTGAGTTA CGAGATACAG 
AAAGGCGGAA AGGATGAGTC CTCTGCACCA CCGCTTCCCA AAACGGGGCC ACCAGCCACA 
GTTCCCAGGA AAGTATATCG CCAGGATTTG GTCATCAACG TAGAGCCAGC ACCCAGTTTA 
ACCAGGGACT ATCAGAGATC TCTGAGTGGT GGTACTCCCC GGAAGCCTAG CGATTGGCGC 
AAGGATGAAA AGTCGGAGAA ATCAGTGCGC GACAAGATTG CCATGTTCTC GTCAAACAAC 
GAACTGGATG CCATACCGCC AGCTCCGGCA ACAGCCCCCA TTTCAAGCTC CTTCACCCGG 
AAGCCCTTGA ATAGGAGCAG TGAAAATCTT CTGGACAGTT GCTCATCATC CTCGGCTCCT 
TCGCTGAAAA CTCGCGCGAT GAGCGTGGAA AATTTGAATG ATGTTCAGCG GCAATATCAG 
TTGGCCAAAC AGCTGCCCCA GTTGCACGTG GCCGACTCTA TGTATTCCCT GAACACAGCC 
ACGCCCACAC CGAGCTACGC CTCTTTGCCG AGAAGAAGCC ACGGGGGATC TTATTCTTCT 
GGAGTGGAGA GAAGGATCAG TTTCTCGGGA GAAGGCGGAG ATGCGGCCAA CCGAAAGGCA 
GCCATTACAA ATATCCTAGA GCAAAGAAGA AGAAGTTTGT CGAAACTGCG AGGATTGGTC 
ATACCAGAGA GACCCCAACT GCTGGAACCC ATCCTGGATC TGCCGGAGAT CAAAAGCCAG 
GTAAAAGCAG CCAGCGGAGA GGATAGTACG GATAGCGGAT TAGGCGAAAG CCACCGCAGT 
ACGGTGAATA GGAATTGCCA ATTGGGTGCA GGAGGTGCCG CAAATAACTA TCGCAGCATT 
TTAAATACCA ATCAAAGAAG ACCCCTGGAG CAACAGCTCT CTCAACCACC AGCCAAACCA 
CCCAGGACAT CGTTGACACC ACTTCAGCCG AGAAGCATGA TGATACCACC ACCACCACCT 
CCTTTAGATC AGGAAAGTGA TACGGATTCG GTGTTTTCGC ACACTGCTAG GGTTGCCACT 
CCGCCGGAGA AGTTTGCACT AACCAGAACC CTCAGCTCCG AAACAAACAC TTCGATAGCT 
AGTTCAAACA CCTCTACTCT AACATCAGGA TCTTCTGCCG GATCTCAAGC CAGTTGCAGT 
TCGCTGGGCA GCACACCAGC GGTGGATCTA ACCAGGCGGG TTCTCAAGAG CCAGGTGATC 
AATGGCGAAG CCGTTGCGCT CTCCAGTCGC AAAAGTATTC TGGCCTCGGC CAAATGTCGC 
AGTGCCAAGA GTCGAGGTCA AGAGGAGGAT AACGACAGCA CCGATGGTGA AGCCTGTTCC 
CTGGCCAATC GTAGAATGAA GCCGATTTCC AGCTATAAGC TCCAGCAGCA ACAGATCCAA 
CTGGGCAAAC AACTGGTAGT GGATAAGCTG ATCAATGTGG CCGCTTATGT GGAACTAACC 
TCGGATACGG ACGACAGCAG CCGTAGATCT GATACGCCTG CCAAGATCAG TGCCATGTTC 
ATAGACGAGG AGCGCAAGGC CAGCTTCAAA GGAGATCCTA ACCAGCAGGC TAAGGTCAAA 
GTGGAGCAGG TCAAACCTAT GGTCCTCCCA ATGCTGCTAC CATCTGCGAA AAGGGAACCG 
CTGAAGCAAC AGACCACCGC CGAACTGCGC GAAAAGTTCG AAAGGAGTGC TGCAGCCCAA 
GCTCAAACCC AAAACCACTC GCCCGTAATC CATAAGGTCA CCCAGAAGCC GCATCATGAG 
CGATTCTCCT CGCTGGACTC CCTGGCGTCC AGTTCCTCCG GCGTTAGTTC CACCACCCAA 
AACGTGAGCA CCACCCAGGA AACGGCCACC GAATTCGGCA GCTTTTCCTC GCTGGGTAGC 
AACCAGAGTT TGATCACCGC CCAGGATGTC CAGCAGATAG TCGAGGAGGC TGATCCTCCA 
CTAAAGACCC CAGAAGCGTT CATCATCGTC CTGCAGAGGG AAAATCCCGA GAGCAGCATC 
GGTATCACTT TGGCCGGCGG TTCTGATTAT GAGGCTAAGG AAATTACGAT CCATAAGATC 
CTGAGCAACA CGCCAGCTGC CAAGGATGGA CGTCTAAAGA AGGGTGATCG AATCCTTGCC 
GTCAATGGAA TGAGCATGCG CGGACTGACG CACCGCGAAT CCATCAGTGT GCTTAAGACC 
CCCCGACCTG AGGTCGTGCT AGTGGTGACC CGCTCCGAGT CGCTGGTGGT GAAGGCGCTG 
ACCAAGAAAC GATCCTCCCT GGGATCACTT AGCTCGCTCA ATGAGAAACC CACGGAGCTG 
GACTATGAAC GCAAGAGGAA CTACCACAAG GCCTCCCGAT CTCTGGACTT GGATCTTGAT 
CTGGTGTCCA ATGAGGCTGG TGAATCGCCA GTGGCTACTA CTCCCAGCAC GGGATCCGTA 
AGTCCACCGC AGCCGGCGAG CTTGCACGAC GAGGATGCGG AGGCCACCAT CGCAGGTATC 
CGGGCCAGGA GGCAGTTGTC CCGCGGAGAT GCCGCCAAGC TTAGCACGAG CGAGCTCCTG 
GAACGGGCGG CAGAGGCCAG AAATGCCATC GCAGCTGAGA TTCGTGCACA AGCGGAGGAT 
GCGGCGGCCA GTGGAGGTGG AGCTCGTTGT GTGGAGATTG TTAAGGACAG CTGTGGACTG 
GGTTTCTCCA TCGAAGGAGG CTTTGATTCG CCGCTGGGAA ATCGTCCCCT TATTGTCAAA 
AAGGTGTTCA TGGGTGGTGC CGCCCAGAAG ACCAACCAGG TGCGCAATGG CGACGAAATC 
CTTAGCATCA ATGGTGCCTC CACGTCGCGG ATGACACGTG TGGATGCCTG GAACTATATG 
AAGCAACTGC CGCTGGGACC GGTCAAGATT TGTTTCGCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bbg ( NM_001144479.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137951.1 Drosophila melanogaster big bang (bbg), transcript variant G, mRNA.

Price & Availability↑

CloneID	OFa18499
Clone ID Related Accession (Same CDS sequence)	NM_001144480.3 , NM_001144478.4 , NM_001144479.4
Accession Version	NM_001144479.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	big bang, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001144479.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGCTGC TGAGCAGCAA CACAGGAACA ACAACGACAA CGACGACAGC AACGCACCAC 
CTGCATCAGG CAACCACCAC TAAACAGCTG TTGGTGCAGG ATTACCTCAG CTACGCCTCA 
CCACCCACAT ATTCCCGCCT GCCGCCCGAT GGTCATGAAT TTCCACCCAA TTTCAGCGAA 
CCACTGATCA TGCACAGCCA CCCACTAAAG GTCACCACCG AGTTGAGTTA CGAGATACAG 
AAAGGCGGAA AGGATGAGTC CTCTGCACCA CCGCTTCCCA AAACGGGGCC ACCAGCCACA 
GTTCCCAGGA AAGTATATCG CCAGGATTTG GTCATCAACG TAGAGCCAGC ACCCAGTTTA 
ACCAGGGACT ATCAGAGATC TCTGAGTGGT GGTACTCCCC GGAAGCCTAG CGATTGGCGC 
AAGGATGAAA AGTCGGAGAA ATCAGTGCGC GACAAGATTG CCATGTTCTC GTCAAACAAC 
GAACTGGATG CCATACCGCC AGCTCCGGCA ACAGCCCCCA TTTCAAGCTC CTTCACCCGG 
AAGCCCTTGA ATAGGAGCAG TGAAAATCTT CTGGACAGTT GCTCATCATC CTCGGCTCCT 
TCGCTGAAAA CTCGCGCGAT GAGCGTGGAA AATTTGAATG ATGTTCAGCG GCAATATCAG 
TTGGCCAAAC AGCTGCCCCA GTTGCACGTG GCCGACTCTA TGTATTCCCT GAACACAGCC 
ACGCCCACAC CGAGCTACGC CTCTTTGCCG AGAAGAAGCC ACGGGGGATC TTATTCTTCT 
GGAGTGGAGA GAAGGATCAG TTTCTCGGGA GAAGGCGGAG ATGCGGCCAA CCGAAAGGCA 
GCCATTACAA ATATCCTAGA GCAAAGAAGA AGAAGTTTGT CGAAACTGCG AGGATTGGTC 
ATACCAGAGA GACCCCAACT GCTGGAACCC ATCCTGGATC TGCCGGAGAT CAAAAGCCAG 
GTAAAAGCAG CCAGCGGAGA GGATAGTACG GATAGCGGAT TAGGCGAAAG CCACCGCAGT 
ACGGTGAATA GGAATTGCCA ATTGGGTGCA GGAGGTGCCG CAAATAACTA TCGCAGCATT 
TTAAATACCA ATCAAAGAAG ACCCCTGGAG CAACAGCTCT CTCAACCACC AGCCAAACCA 
CCCAGGACAT CGTTGACACC ACTTCAGCCG AGAAGCATGA TGATACCACC ACCACCACCT 
CCTTTAGATC AGGAAAGTGA TACGGATTCG GTGTTTTCGC ACACTGCTAG GGTTGCCACT 
CCGCCGGAGA AGTTTGCACT AACCAGAACC CTCAGCTCCG AAACAAACAC TTCGATAGCT 
AGTTCAAACA CCTCTACTCT AACATCAGGA TCTTCTGCCG GATCTCAAGC CAGTTGCAGT 
TCGCTGGGCA GCACACCAGC GGTGGATCTA ACCAGGCGGG TTCTCAAGAG CCAGGTGATC 
AATGGCGAAG CCGTTGCGCT CTCCAGTCGC AAAAGTATTC TGGCCTCGGC CAAATGTCGC 
AGTGCCAAGA GTCGAGGTCA AGAGGAGGAT AACGACAGCA CCGATGGTGA AGCCTGTTCC 
CTGGCCAATC GTAGAATGAA GCCGATTTCC AGCTATAAGC TCCAGCAGCA ACAGATCCAA 
CTGGGCAAAC AACTGGTAGT GGATAAGCTG ATCAATGTGG CCGCTTATGT GGAACTAACC 
TCGGATACGG ACGACAGCAG CCGTAGATCT GATACGCCTG CCAAGATCAG TGCCATGTTC 
ATAGACGAGG AGCGCAAGGC CAGCTTCAAA GGAGATCCTA ACCAGCAGGC TAAGGTCAAA 
GTGGAGCAGG TCAAACCTAT GGTCCTCCCA ATGCTGCTAC CATCTGCGAA AAGGGAACCG 
CTGAAGCAAC AGACCACCGC CGAACTGCGC GAAAAGTTCG AAAGGAGTGC TGCAGCCCAA 
GCTCAAACCC AAAACCACTC GCCCGTAATC CATAAGGTCA CCCAGAAGCC GCATCATGAG 
CGATTCTCCT CGCTGGACTC CCTGGCGTCC AGTTCCTCCG GCGTTAGTTC CACCACCCAA 
AACGTGAGCA CCACCCAGGA AACGGCCACC GAATTCGGCA GCTTTTCCTC GCTGGGTAGC 
AACCAGAGTT TGATCACCGC CCAGGATGTC CAGCAGATAG TCGAGGAGGC TGATCCTCCA 
CTAAAGACCC CAGAAGCGTT CATCATCGTC CTGCAGAGGG AAAATCCCGA GAGCAGCATC 
GGTATCACTT TGGCCGGCGG TTCTGATTAT GAGGCTAAGG AAATTACGAT CCATAAGATC 
CTGAGCAACA CGCCAGCTGC CAAGGATGGA CGTCTAAAGA AGGGTGATCG AATCCTTGCC 
GTCAATGGAA TGAGCATGCG CGGACTGACG CACCGCGAAT CCATCAGTGT GCTTAAGACC 
CCCCGACCTG AGGTCGTGCT AGTGGTGACC CGCTCCGAGT CGCTGGTGGT GAAGGCGCTG 
ACCAAGAAAC GATCCTCCCT GGGATCACTT AGCTCGCTCA ATGAGAAACC CACGGAGCTG 
GACTATGAAC GCAAGAGGAA CTACCACAAG GCCTCCCGAT CTCTGGACTT GGATCTTGAT 
CTGGTGTCCA ATGAGGCTGG TGAATCGCCA GTGGCTACTA CTCCCAGCAC GGGATCCGTA 
AGTCCACCGC AGCCGGCGAG CTTGCACGAC GAGGATGCGG AGGCCACCAT CGCAGGTATC 
CGGGCCAGGA GGCAGTTGTC CCGCGGAGAT GCCGCCAAGC TTAGCACGAG CGAGCTCCTG 
GAACGGGCGG CAGAGGCCAG AAATGCCATC GCAGCTGAGA TTCGTGCACA AGCGGAGGAT 
GCGGCGGCCA GTGGAGGTGG AGCTCGTTGT GTGGAGATTG TTAAGGACAG CTGTGGACTG 
GGTTTCTCCA TCGAAGGAGG CTTTGATTCG CCGCTGGGAA ATCGTCCCCT TATTGTCAAA 
AAGGTGTTCA TGGGTGGTGC CGCCCAGAAG ACCAACCAGG TGCGCAATGG CGACGAAATC 
CTTAGCATCA ATGGTGCCTC CACGTCGCGG ATGACACGTG TGGATGCCTG GAACTATATG 
AAGCAACTGC CGCTGGGACC GGTCAAGATT TGTTTCGCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137951.1
CDS	586..3687
Translation	MELLSSNTGTTTTTTTATHHLHQATTTKQLLVQDYLSYASPPTYSRLPPDGHEFPPNFSEPLIMHSHPLKVTTELSYEIQKGGKDESSAPPLPKTGPPATVPRKVYRQDLVINVEPAPSLTRDYQRSLSGGTPRKPSDWRKDEKSEKSVRDKIAMFSSNNELDAIPPAPATAPISSSFTRKPLNRSSENLLDSCSSSSAPSLKTRAMSVENLNDVQRQYQLAKQLPQLHVADSMYSLNTATPTPSYASLPRRSHGGSYSSGVERRISFSGEGGDAANRKAAITNILEQRRRSLSKLRGLVIPERPQLLEPILDLPEIKSQVKAASGEDSTDSGLGESHRSTVNRNCQLGAGGAANNYRSILNTNQRRPLEQQLSQPPAKPPRTSLTPLQPRSMMIPPPPPPLDQESDTDSVFSHTARVATPPEKFALTRTLSSETNTSIASSNTSTLTSGSSAGSQASCSSLGSTPAVDLTRRVLKSQVINGEAVALSSRKSILASAKCRSAKSRGQEEDNDSTDGEACSLANRRMKPISSYKLQQQQIQLGKQLVVDKLINVAAYVELTSDTDDSSRRSDTPAKISAMFIDEERKASFKGDPNQQAKVKVEQVKPMVLPMLLPSAKREPLKQQTTAELREKFERSAAAQAQTQNHSPVIHKVTQKPHHERFSSLDSLASSSSGVSSTTQNVSTTQETATEFGSFSSLGSNQSLITAQDVQQIVEEADPPLKTPEAFIIVLQRENPESSIGITLAGGSDYEAKEITIHKILSNTPAAKDGRLKKGDRILAVNGMSMRGLTHRESISVLKTPRPEVVLVVTRSESLVVKALTKKRSSLGSLSSLNEKPTELDYERKRNYHKASRSLDLDLDLVSNEAGESPVATTPSTGSVSPPQPASLHDEDAEATIAGIRARRQLSRGDAAKLSTSELLERAAEARNAIAAEIRAQAEDAAASGGGARCVEIVKDSCGLGFSIEGGFDSPLGNRPLIVKKVFMGGAAQKTNQVRNGDEILSINGASTSRMTRVDAWNYMKQLPLGPVKICFA

Target ORF information:

RefSeq Version	NM_001144479.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster big bang (bbg), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144479.4

ORF Insert Sequence:

ATGGAGCTGC TGAGCAGCAA CACAGGAACA ACAACGACAA CGACGACAGC AACGCACCAC 
CTGCATCAGG CAACCACCAC TAAACAGCTG TTGGTGCAGG ATTACCTCAG CTACGCCTCA 
CCACCCACAT ATTCCCGCCT GCCGCCCGAT GGTCATGAAT TTCCACCCAA TTTCAGCGAA 
CCACTGATCA TGCACAGCCA CCCACTAAAG GTCACCACCG AGTTGAGTTA CGAGATACAG 
AAAGGCGGAA AGGATGAGTC CTCTGCACCA CCGCTTCCCA AAACGGGGCC ACCAGCCACA 
GTTCCCAGGA AAGTATATCG CCAGGATTTG GTCATCAACG TAGAGCCAGC ACCCAGTTTA 
ACCAGGGACT ATCAGAGATC TCTGAGTGGT GGTACTCCCC GGAAGCCTAG CGATTGGCGC 
AAGGATGAAA AGTCGGAGAA ATCAGTGCGC GACAAGATTG CCATGTTCTC GTCAAACAAC 
GAACTGGATG CCATACCGCC AGCTCCGGCA ACAGCCCCCA TTTCAAGCTC CTTCACCCGG 
AAGCCCTTGA ATAGGAGCAG TGAAAATCTT CTGGACAGTT GCTCATCATC CTCGGCTCCT 
TCGCTGAAAA CTCGCGCGAT GAGCGTGGAA AATTTGAATG ATGTTCAGCG GCAATATCAG 
TTGGCCAAAC AGCTGCCCCA GTTGCACGTG GCCGACTCTA TGTATTCCCT GAACACAGCC 
ACGCCCACAC CGAGCTACGC CTCTTTGCCG AGAAGAAGCC ACGGGGGATC TTATTCTTCT 
GGAGTGGAGA GAAGGATCAG TTTCTCGGGA GAAGGCGGAG ATGCGGCCAA CCGAAAGGCA 
GCCATTACAA ATATCCTAGA GCAAAGAAGA AGAAGTTTGT CGAAACTGCG AGGATTGGTC 
ATACCAGAGA GACCCCAACT GCTGGAACCC ATCCTGGATC TGCCGGAGAT CAAAAGCCAG 
GTAAAAGCAG CCAGCGGAGA GGATAGTACG GATAGCGGAT TAGGCGAAAG CCACCGCAGT 
ACGGTGAATA GGAATTGCCA ATTGGGTGCA GGAGGTGCCG CAAATAACTA TCGCAGCATT 
TTAAATACCA ATCAAAGAAG ACCCCTGGAG CAACAGCTCT CTCAACCACC AGCCAAACCA 
CCCAGGACAT CGTTGACACC ACTTCAGCCG AGAAGCATGA TGATACCACC ACCACCACCT 
CCTTTAGATC AGGAAAGTGA TACGGATTCG GTGTTTTCGC ACACTGCTAG GGTTGCCACT 
CCGCCGGAGA AGTTTGCACT AACCAGAACC CTCAGCTCCG AAACAAACAC TTCGATAGCT 
AGTTCAAACA CCTCTACTCT AACATCAGGA TCTTCTGCCG GATCTCAAGC CAGTTGCAGT 
TCGCTGGGCA GCACACCAGC GGTGGATCTA ACCAGGCGGG TTCTCAAGAG CCAGGTGATC 
AATGGCGAAG CCGTTGCGCT CTCCAGTCGC AAAAGTATTC TGGCCTCGGC CAAATGTCGC 
AGTGCCAAGA GTCGAGGTCA AGAGGAGGAT AACGACAGCA CCGATGGTGA AGCCTGTTCC 
CTGGCCAATC GTAGAATGAA GCCGATTTCC AGCTATAAGC TCCAGCAGCA ACAGATCCAA 
CTGGGCAAAC AACTGGTAGT GGATAAGCTG ATCAATGTGG CCGCTTATGT GGAACTAACC 
TCGGATACGG ACGACAGCAG CCGTAGATCT GATACGCCTG CCAAGATCAG TGCCATGTTC 
ATAGACGAGG AGCGCAAGGC CAGCTTCAAA GGAGATCCTA ACCAGCAGGC TAAGGTCAAA 
GTGGAGCAGG TCAAACCTAT GGTCCTCCCA ATGCTGCTAC CATCTGCGAA AAGGGAACCG 
CTGAAGCAAC AGACCACCGC CGAACTGCGC GAAAAGTTCG AAAGGAGTGC TGCAGCCCAA 
GCTCAAACCC AAAACCACTC GCCCGTAATC CATAAGGTCA CCCAGAAGCC GCATCATGAG 
CGATTCTCCT CGCTGGACTC CCTGGCGTCC AGTTCCTCCG GCGTTAGTTC CACCACCCAA 
AACGTGAGCA CCACCCAGGA AACGGCCACC GAATTCGGCA GCTTTTCCTC GCTGGGTAGC 
AACCAGAGTT TGATCACCGC CCAGGATGTC CAGCAGATAG TCGAGGAGGC TGATCCTCCA 
CTAAAGACCC CAGAAGCGTT CATCATCGTC CTGCAGAGGG AAAATCCCGA GAGCAGCATC 
GGTATCACTT TGGCCGGCGG TTCTGATTAT GAGGCTAAGG AAATTACGAT CCATAAGATC 
CTGAGCAACA CGCCAGCTGC CAAGGATGGA CGTCTAAAGA AGGGTGATCG AATCCTTGCC 
GTCAATGGAA TGAGCATGCG CGGACTGACG CACCGCGAAT CCATCAGTGT GCTTAAGACC 
CCCCGACCTG AGGTCGTGCT AGTGGTGACC CGCTCCGAGT CGCTGGTGGT GAAGGCGCTG 
ACCAAGAAAC GATCCTCCCT GGGATCACTT AGCTCGCTCA ATGAGAAACC CACGGAGCTG 
GACTATGAAC GCAAGAGGAA CTACCACAAG GCCTCCCGAT CTCTGGACTT GGATCTTGAT 
CTGGTGTCCA ATGAGGCTGG TGAATCGCCA GTGGCTACTA CTCCCAGCAC GGGATCCGTA 
AGTCCACCGC AGCCGGCGAG CTTGCACGAC GAGGATGCGG AGGCCACCAT CGCAGGTATC 
CGGGCCAGGA GGCAGTTGTC CCGCGGAGAT GCCGCCAAGC TTAGCACGAG CGAGCTCCTG 
GAACGGGCGG CAGAGGCCAG AAATGCCATC GCAGCTGAGA TTCGTGCACA AGCGGAGGAT 
GCGGCGGCCA GTGGAGGTGG AGCTCGTTGT GTGGAGATTG TTAAGGACAG CTGTGGACTG 
GGTTTCTCCA TCGAAGGAGG CTTTGATTCG CCGCTGGGAA ATCGTCCCCT TATTGTCAAA 
AAGGTGTTCA TGGGTGGTGC CGCCCAGAAG ACCAACCAGG TGCGCAATGG CGACGAAATC 
CTTAGCATCA ATGGTGCCTC CACGTCGCGG ATGACACGTG TGGATGCCTG GAACTATATG 
AAGCAACTGC CGCTGGGACC GGTCAAGATT TGTTTCGCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bbg ( NM_001144477.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137949.1 Drosophila melanogaster big bang (bbg), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa18502
Clone ID Related Accession (Same CDS sequence)	NM_001144477.2
Accession Version	NM_001144477.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	big bang, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On May 8, 2012 this sequence version replaced NM_001144477.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTATCGCC TACAGAGACG AACTGCTTTG ATAACCAAAC AAGATCTTGG TACCCTCAGT 
AACCACCACA GCAATATAAG CAGCATTAGC GGCATTAACG CCAGTAGCAA CATCCCGGCA 
GCAACTTTAA TCAGCAGCAG CATTAAGAAG ACATCCACTG AGGCGGCAAC CACAAAACCT 
GCTGCACCCC AGATCCCCGG CAAAATGGAG CTGCTGAGCA GCAACACAGG AACAACAACG 
ACAACGACGA CAGCAACGCA CCACCTGCAT CAGGCAACCA CCACTAAACA GCTGTTGGTG 
CAGGATTACC TCAGCTACGC CTCACCACCC ACATATTCCC GCCTGCCGCC CGATGGTCAT 
GAATTTCCAC CCAATTTCAG CGAACCACTG ATCATGCACA GCCACCCACT AAAGGTCACC 
ACCGAGTTGA GTTACGAGAT ACAGAAAGGC GGAAAGGATG AGTCCTCTGC ACCACCGCTT 
CCCAAAACGG GGCCACCAGC CACAGTTCCC AGGAAAGTAT ATCGCCAGGA TTTGGTCATC 
AACGTAGAGC CAGCACCCAG TTTAACCAGG GACTATCAGA GATCTCTGAG TGGTGGTACT 
CCCCGGAAGC CTAGCGATTG GCGCAAGGAT GAAAAGTCGG AGAAATCAGT GCGCGACAAG 
ATTGCCATGT TCTCGTCAAA CAACGAACTG GATGCCATAC CGCCAGCTCC GGCAACAGCC 
CCCATTTCAA GCTCCTTCAC CCGGAAGCCC TTGAATAGGA GCAGTGAAAA TCTTCTGGAC 
AGTTGCTCAT CATCCTCGGC TCCTTCGCTG AAAACTCGCG CGATGAGCGT GGAAAATTTG 
AATGATGTTC AGCGGCAATA TCAGTTGGCC AAACAGCTGC CCCAGTTGCA CGTGGCCGAC 
TCTATGTATT CCCTGAACAC AGCCACGCCC ACACCGAGCT ACGCCTCTTT GCCGAGAAGA 
AGCCACGGGG GATCTTATTC TTCTGGAGTG GAGAGAAGGA TCAGTTTCTC GGGAGAAGGC 
GGAGATGCGG CCAACCGAAA GGCAGCCATT ACAAATATCC TAGAGCAAAG AAGAAGAAGT 
TTGTCGAAAC TGCGAGGATT GGTCATACCA GAGAGACCCC AACTGCTGGA ACCCATCCTG 
GATCTGCCGG AGATCAAAAG CCAGGTAAAA GCAGCCAGCG GAGAGGATAG TACGGATAGC 
GGATTAGGCG AAAGCCACCG CAGTACGGTG AATAGGAATT GCCAATTGGG TGCAGGAGGT 
GCCGCAAATA ACTATCGCAG CATTTTAAAT ACCAATCAAA GAAGACCCCT GGAGCAACAG 
CTCTCTCAAC CACCAGCCAA ACCACCCAGG ACATCGTTGA CACCACTTCA GCCGAGAAGC 
ATGATGATAC CACCACCACC ACCTCCTTTA GATCAGGAAA GTGATACGGA TTCGGTGTTT 
TCGCACACTG CTAGGGTTGC CACTCCGCCG GAGAAGTTTG CACTAACCAG AACCCTCAGC 
TCCGAAACAA ACACTTCGAT AGCTAGTTCA AACACCTCTA CTCTAACATC AGGATCTTCT 
GCCGGATCTC AAGCCAGTTG CAGTTCGCTG GGCAGCACAC CAGCGGTGGA TCTAACCAGG 
CGGGTTCTCA AGAGCCAGGT GATCAATGGC GAAGCCGTTG CGCTCTCCAG TCGCAAAAGT 
ATTCTGGCCT CGGCCAAATG TCGCAGTGCC AAGAGTCGAG GTCAAGAGGA GGATAACGAC 
AGCACCGATG GTGAAGCCTG TTCCCTGGCC AATCGTAGAA TGAAGCCGAT TTCCAGCTAT 
AAGCTCCAGC AGCAACAGAT CCAACTGGGC AAACAACTGG TAGTGGATAA GCTGATCAAT 
GTGGCCGCTT ATGTGGAACT AACCTCGGAT ACGGACGACA GCAGCCGTAG ATCTGATACG 
CCTGCCAAGA TCAGTGCCAT GTTCATAGAC GAGGAGCGCA AGGCCAGCTT CAAAGGAGAT 
CCTAACCAGC AGGCTAAGGT CAAAGTGGAG CAGGTCAAAC CTATGGTCCT CCCAATGCTG 
CTACCATCTG CGAAAAGGGA ACCGCTGAAG CAACAGACCA CCGCCGAACT GCGCGAAAAG 
TTCGAAAGGA GTGCTGCAGC CCAAGCTCAA ACCCAAAACC ACTCGCCCGT AATCCATAAG 
GTCACCCAGA AGCCGCATCA TGAGCGATTC TCCTCGCTGG ACTCCCTGGC GTCCAGTTCC 
TCCGGCGTTA GTTCCACCAC CCAAAACGTG AGCACCACCC AGGAAACGGC CACCGAATTC 
GGCAGCTTTT CCTCGCTGGG TAGCAACCAG AGTTTGATCA CCGCCCAGGA TGTCCAGCAG 
ATAGTCGAGG AGGCTGATCC TCCACTAAAG ACCCCAGAAG CGTTCATCAT CGTCCTGCAG 
AGGGAAAATC CCGAGAGCAG CATCGGTATC ACTTTGGCCG GCGGTTCTGA TTATGAGGCT 
AAGGAAATTA CGATCCATAA GATCCTGAGC AACACGCCAG CTGCCAAGGA TGGACGTCTA 
AAGAAGGGTG ATCGAATCCT TGCCGTCAAT GGAATGAGCA TGCGCGGACT GACGCACCGC 
GAATCCATCA GTGTGCTTAA GACCCCCCGA CCTGAGGTCG TGCTAGTGGT GACCCGCTCC 
GAGTCGCTGG TGGTGAAGGC GCTGACCAAG AAACGATCCT CCCTGGGATC ACTTAGCTCG 
CTCAATGAGA AACCCACGGA GCTGGACTAT GAACGCAAGA GGAACTACCA CAAGGCCTCC 
CGATCTCTGG ACTTGGATCT TGATCTGGTG TCCAATGAGG CTGGTGAATC GCCAGTGGCT 
ACTACTCCCA GCACGGGATC CGTAAGTCCA CCGCAGCCGG CGAGCTTGCA CGACGAGGAT 
GCGGAGGCCA CCATCGCAGG TATCCGGGCC AGGAGGCAGT TGTCCCGCGG AGATGCCGCC 
AAGCTTAGCA CGAGCGAGCT CCTGGAACGG GCGGCAGAGG CCAGAAATGC CATCGCAGCT 
GAGATTCGTG CACAAGCGGA GGATGCGGCG GCCAGTGGAG GTGGAGCTCG TTGTGTGGAG 
ATTGTTAAGG ACAGCTGTGG ACTGGGTTTC TCCATCGAAG GAGGCTTTGA TTCGCCGCTG 
GGAAATCGTC CCCTTATTGT CAAAAAGGTG TTCATGGGTG GTGCCGCCCA GAAGACCAAC 
CAGGTGCGCA ATGGCGACGA AATCCTTAGC ATCAATGGTG CCTCCACGTC GCGGATGACA 
CGTGTGGATG CCTGGAACTA TATGAAGCAA CTGCCGCTGG GACCGGTCAA GATTTGTTTC 
GCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137949.1
CDS	273..3578
Translation	MYRLQRRTALITKQDLGTLSNHHSNISSISGINASSNIPAATLISSSIKKTSTEAATTKPAAPQIPGKMELLSSNTGTTTTTTTATHHLHQATTTKQLLVQDYLSYASPPTYSRLPPDGHEFPPNFSEPLIMHSHPLKVTTELSYEIQKGGKDESSAPPLPKTGPPATVPRKVYRQDLVINVEPAPSLTRDYQRSLSGGTPRKPSDWRKDEKSEKSVRDKIAMFSSNNELDAIPPAPATAPISSSFTRKPLNRSSENLLDSCSSSSAPSLKTRAMSVENLNDVQRQYQLAKQLPQLHVADSMYSLNTATPTPSYASLPRRSHGGSYSSGVERRISFSGEGGDAANRKAAITNILEQRRRSLSKLRGLVIPERPQLLEPILDLPEIKSQVKAASGEDSTDSGLGESHRSTVNRNCQLGAGGAANNYRSILNTNQRRPLEQQLSQPPAKPPRTSLTPLQPRSMMIPPPPPPLDQESDTDSVFSHTARVATPPEKFALTRTLSSETNTSIASSNTSTLTSGSSAGSQASCSSLGSTPAVDLTRRVLKSQVINGEAVALSSRKSILASAKCRSAKSRGQEEDNDSTDGEACSLANRRMKPISSYKLQQQQIQLGKQLVVDKLINVAAYVELTSDTDDSSRRSDTPAKISAMFIDEERKASFKGDPNQQAKVKVEQVKPMVLPMLLPSAKREPLKQQTTAELREKFERSAAAQAQTQNHSPVIHKVTQKPHHERFSSLDSLASSSSGVSSTTQNVSTTQETATEFGSFSSLGSNQSLITAQDVQQIVEEADPPLKTPEAFIIVLQRENPESSIGITLAGGSDYEAKEITIHKILSNTPAAKDGRLKKGDRILAVNGMSMRGLTHRESISVLKTPRPEVVLVVTRSESLVVKALTKKRSSLGSLSSLNEKPTELDYERKRNYHKASRSLDLDLDLVSNEAGESPVATTPSTGSVSPPQPASLHDEDAEATIAGIRARRQLSRGDAAKLSTSELLERAAEARNAIAAEIRAQAEDAAASGGGARCVEIVKDSCGLGFSIEGGFDSPLGNRPLIVKKVFMGGAAQKTNQVRNGDEILSINGASTSRMTRVDAWNYMKQLPLGPVKICFA

Target ORF information:

RefSeq Version	NM_001144477.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster big bang (bbg), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144477.2

ORF Insert Sequence:

ATGTATCGCC TACAGAGACG AACTGCTTTG ATAACCAAAC AAGATCTTGG TACCCTCAGT 
AACCACCACA GCAATATAAG CAGCATTAGC GGCATTAACG CCAGTAGCAA CATCCCGGCA 
GCAACTTTAA TCAGCAGCAG CATTAAGAAG ACATCCACTG AGGCGGCAAC CACAAAACCT 
GCTGCACCCC AGATCCCCGG CAAAATGGAG CTGCTGAGCA GCAACACAGG AACAACAACG 
ACAACGACGA CAGCAACGCA CCACCTGCAT CAGGCAACCA CCACTAAACA GCTGTTGGTG 
CAGGATTACC TCAGCTACGC CTCACCACCC ACATATTCCC GCCTGCCGCC CGATGGTCAT 
GAATTTCCAC CCAATTTCAG CGAACCACTG ATCATGCACA GCCACCCACT AAAGGTCACC 
ACCGAGTTGA GTTACGAGAT ACAGAAAGGC GGAAAGGATG AGTCCTCTGC ACCACCGCTT 
CCCAAAACGG GGCCACCAGC CACAGTTCCC AGGAAAGTAT ATCGCCAGGA TTTGGTCATC 
AACGTAGAGC CAGCACCCAG TTTAACCAGG GACTATCAGA GATCTCTGAG TGGTGGTACT 
CCCCGGAAGC CTAGCGATTG GCGCAAGGAT GAAAAGTCGG AGAAATCAGT GCGCGACAAG 
ATTGCCATGT TCTCGTCAAA CAACGAACTG GATGCCATAC CGCCAGCTCC GGCAACAGCC 
CCCATTTCAA GCTCCTTCAC CCGGAAGCCC TTGAATAGGA GCAGTGAAAA TCTTCTGGAC 
AGTTGCTCAT CATCCTCGGC TCCTTCGCTG AAAACTCGCG CGATGAGCGT GGAAAATTTG 
AATGATGTTC AGCGGCAATA TCAGTTGGCC AAACAGCTGC CCCAGTTGCA CGTGGCCGAC 
TCTATGTATT CCCTGAACAC AGCCACGCCC ACACCGAGCT ACGCCTCTTT GCCGAGAAGA 
AGCCACGGGG GATCTTATTC TTCTGGAGTG GAGAGAAGGA TCAGTTTCTC GGGAGAAGGC 
GGAGATGCGG CCAACCGAAA GGCAGCCATT ACAAATATCC TAGAGCAAAG AAGAAGAAGT 
TTGTCGAAAC TGCGAGGATT GGTCATACCA GAGAGACCCC AACTGCTGGA ACCCATCCTG 
GATCTGCCGG AGATCAAAAG CCAGGTAAAA GCAGCCAGCG GAGAGGATAG TACGGATAGC 
GGATTAGGCG AAAGCCACCG CAGTACGGTG AATAGGAATT GCCAATTGGG TGCAGGAGGT 
GCCGCAAATA ACTATCGCAG CATTTTAAAT ACCAATCAAA GAAGACCCCT GGAGCAACAG 
CTCTCTCAAC CACCAGCCAA ACCACCCAGG ACATCGTTGA CACCACTTCA GCCGAGAAGC 
ATGATGATAC CACCACCACC ACCTCCTTTA GATCAGGAAA GTGATACGGA TTCGGTGTTT 
TCGCACACTG CTAGGGTTGC CACTCCGCCG GAGAAGTTTG CACTAACCAG AACCCTCAGC 
TCCGAAACAA ACACTTCGAT AGCTAGTTCA AACACCTCTA CTCTAACATC AGGATCTTCT 
GCCGGATCTC AAGCCAGTTG CAGTTCGCTG GGCAGCACAC CAGCGGTGGA TCTAACCAGG 
CGGGTTCTCA AGAGCCAGGT GATCAATGGC GAAGCCGTTG CGCTCTCCAG TCGCAAAAGT 
ATTCTGGCCT CGGCCAAATG TCGCAGTGCC AAGAGTCGAG GTCAAGAGGA GGATAACGAC 
AGCACCGATG GTGAAGCCTG TTCCCTGGCC AATCGTAGAA TGAAGCCGAT TTCCAGCTAT 
AAGCTCCAGC AGCAACAGAT CCAACTGGGC AAACAACTGG TAGTGGATAA GCTGATCAAT 
GTGGCCGCTT ATGTGGAACT AACCTCGGAT ACGGACGACA GCAGCCGTAG ATCTGATACG 
CCTGCCAAGA TCAGTGCCAT GTTCATAGAC GAGGAGCGCA AGGCCAGCTT CAAAGGAGAT 
CCTAACCAGC AGGCTAAGGT CAAAGTGGAG CAGGTCAAAC CTATGGTCCT CCCAATGCTG 
CTACCATCTG CGAAAAGGGA ACCGCTGAAG CAACAGACCA CCGCCGAACT GCGCGAAAAG 
TTCGAAAGGA GTGCTGCAGC CCAAGCTCAA ACCCAAAACC ACTCGCCCGT AATCCATAAG 
GTCACCCAGA AGCCGCATCA TGAGCGATTC TCCTCGCTGG ACTCCCTGGC GTCCAGTTCC 
TCCGGCGTTA GTTCCACCAC CCAAAACGTG AGCACCACCC AGGAAACGGC CACCGAATTC 
GGCAGCTTTT CCTCGCTGGG TAGCAACCAG AGTTTGATCA CCGCCCAGGA TGTCCAGCAG 
ATAGTCGAGG AGGCTGATCC TCCACTAAAG ACCCCAGAAG CGTTCATCAT CGTCCTGCAG 
AGGGAAAATC CCGAGAGCAG CATCGGTATC ACTTTGGCCG GCGGTTCTGA TTATGAGGCT 
AAGGAAATTA CGATCCATAA GATCCTGAGC AACACGCCAG CTGCCAAGGA TGGACGTCTA 
AAGAAGGGTG ATCGAATCCT TGCCGTCAAT GGAATGAGCA TGCGCGGACT GACGCACCGC 
GAATCCATCA GTGTGCTTAA GACCCCCCGA CCTGAGGTCG TGCTAGTGGT GACCCGCTCC 
GAGTCGCTGG TGGTGAAGGC GCTGACCAAG AAACGATCCT CCCTGGGATC ACTTAGCTCG 
CTCAATGAGA AACCCACGGA GCTGGACTAT GAACGCAAGA GGAACTACCA CAAGGCCTCC 
CGATCTCTGG ACTTGGATCT TGATCTGGTG TCCAATGAGG CTGGTGAATC GCCAGTGGCT 
ACTACTCCCA GCACGGGATC CGTAAGTCCA CCGCAGCCGG CGAGCTTGCA CGACGAGGAT 
GCGGAGGCCA CCATCGCAGG TATCCGGGCC AGGAGGCAGT TGTCCCGCGG AGATGCCGCC 
AAGCTTAGCA CGAGCGAGCT CCTGGAACGG GCGGCAGAGG CCAGAAATGC CATCGCAGCT 
GAGATTCGTG CACAAGCGGA GGATGCGGCG GCCAGTGGAG GTGGAGCTCG TTGTGTGGAG 
ATTGTTAAGG ACAGCTGTGG ACTGGGTTTC TCCATCGAAG GAGGCTTTGA TTCGCCGCTG 
GGAAATCGTC CCCTTATTGT CAAAAAGGTG TTCATGGGTG GTGCCGCCCA GAAGACCAAC 
CAGGTGCGCA ATGGCGACGA AATCCTTAGC ATCAATGGTG CCTCCACGTC GCGGATGACA 
CGTGTGGATG CCTGGAACTA TATGAAGCAA CTGCCGCTGG GACCGGTCAA GATTTGTTTC 
GCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bbg ( NM_001144481.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137953.1 Drosophila melanogaster big bang (bbg), transcript variant J, mRNA.

Price & Availability↑

CloneID	OFa18501
Clone ID Related Accession (Same CDS sequence)	NM_001144481.2
Accession Version	NM_001144481.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5529bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	big bang, isoform J
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On May 8, 2012 this sequence version replaced NM_001144481.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAGTAA ACGCCGGTGG ACTGGCCCAG CTGACTGATG ATAAGATTGC TGACCATGAC 
GCAAGTGCAA CCAGAGCGGA TACTCGCGAC GAAGTCACAA CAAACCAAAC CCAGGAAATC 
AGCTCCAGCA GTTCGAGTTC TGTTAAAAGT CAAAAGACCA CCACAACCAC TCGAGTGACC 
ACCACCAAGA CTAGTTCCAG TTCCAGTTCC TCCAGTTGCT CATCCACTGT TATCCCAGAT 
GTTGCCTTCA AGCTGCAACC CTATGAGGAA AGGGACGATA TACTCTCGGA ACCCATTACC 
ACCATCCACG AAGAACGACG TGTGCTAAGT GAGCAGAAGA CCCTCAGTGA ATCGAGGACT 
CGCGATGCAT TAACCGGCGA AGAGCAGCTG GTAACTAGTG CCAATTCGAA GTCGAGCAGT 
GCCCGCTTCA AGAAGATCAG CAGCAACGAC AACCTACTCG ACCTTGGCGA CGAGCAGGAC 
ATGCAGGAGC AGCCGTTGAC CGCCACGATC ACCCAGGAGT CGAGTCTGAG GGAGCGGTTG 
GAGTGTCCTC AGGATAACCA GAGTATCGAC AGGGGTACTC TGACCCTATC CGAGCGGGGA 
AAGCAGCTGA GCGAGAAAGA AGGCCTCACC ACCTACACGG AATGTGAGAC GAGTGAAAAG 
GAGTCCTACC TAGAGGGCCA ACAAGTCCTC AACGGCAAAG GCGGTTTCGT TAGCCAGGAT 
GAGAATAGTG ACCAACCAAC TTACGAACGA GAGGTCAGCG AGACACTAAC GATCGTCAAC 
GATGGCGAAA AGCAGGTCAC CAAGAAATTG GAACAGAACA AGGAAATTGC TGGCAAAAAG 
GGTGCCAAGC TCCTTAAGAA GACCGATGAG GAGCGACGTC TTGAGCAGGA GGCCCAAAAG 
CTGATCGAAA GCTATCAGAA AGTCAAGAAA GAAGCGGAGA AGCTGTACAA GCTGGAATTG 
GCCGACGATG ATCAGGGCTT TGATCTGAGT GCCTTCGAGC AGGCGGAGGA AGAGGATAAG 
CCAGAAGTTT CTGAGGAAAT CAAAGAAGTG AAGGATTCTC AGCCAGTAAA GGTCGATATT 
CAAATTGAAG AAGAGGTTAA AACACCTAAT CAAGTCATAG GGGAAGTCAA AGTGGAAGAT 
GTGAATAAGG AAATTAAAGA GACGGTACAA GTCTCTCAAC AGGAAATTAA GAAAGAGGTC 
AAGGTCTCAC ACCAAGAAAC AAAAGAAACC TTTAAGAGTG AAGAAAAAGA TGAGAGTAAT 
GAACTCAAAA TAGTTAAGGA GGAGGTCAAA GTAACACATC AGGATCTTAG GGAACAGATC 
CAGGAGATCG AGGCTGTCAA AGTGGTGCAC AAAGAAGTAA AAGTTATTAA GGAAAGTGCT 
AAAGTCACTA CGACCACGAG TCACAAGCAA GCAACTAAAG AAGTAATCAT CTCGAAAGAA 
GATCACAAAA ATGATAGCGC ACTCCCTGCC GTGTCAACCA CAAAAGTAGA GATCATCTCA 
CCTCCCCTGG AAGACGACTT GGGTTATGTC CTCCACAAGC ACATTATTTT TCCCCAGGAA 
AAGGTCAAGA CGGCTCCTAC GCCACTGCCA AAACCGAAAC CCAAACCTCC AGTGCCCACG 
AACAAACCCA AAATGCCGGT CAATTTGACC AAACCGAAAT CAGCACCACC ACCGGTGCCC 
AGTAAGAGGA GTGAGCTATC CGGATCCGGT GGCAAGCCCA CACCCACCCA AAGACGCGGC 
AGCCTGGAGA CCGCCCAGCC TCCAAAACCA CTAGAACGTA TAATTGTCGG TGTGGAGCAG 
CGGGATGAGC CAGAAAAGCC CCAGCCAATT ATCAACTTGG CCAGCGTGGA TCTGCCAAAT 
GTGGCGCCCA ACAGCAAGCA AGCGACAGTG GAAACGACTC CCGTGGTCGA AATGCCACCG 
GATGAGCTCC AATTCGAGAG TCACCAACTG CCGGATGACG GTGAAAAGGA AAAGGACTTG 
CACCAGGAGT CAAATGACCT GCTCAGCGGG TCGTCATCGT CGTCGGTAAC CACGCCCATA 
ACAACGCCAA TTTTGGTATC TGCCACCTCG TCGATGGACT CGGTGCAAAG TGTCATCGAG 
GTCGTCAATG GCATGCCCAT CATCAGCAAT CAGCAAACTG ACGACGACGA CATTGATGAA 
GTTGCAGCGT ATGACGATGG GATGCATTTG GAGGCCGAAG GTACGAAGGA TGTTCGTCCC 
GTCCAGGAGG TGACAACGTT GACGCTCGAC AGGGAACACG AATCAGATCT TGGTACCCTC 
AGTAACCACC ACAGCAATAT AAGCAGCATT AGCGGCATTA ACGCCAGTAG CAACATCCCG 
GCAGCAACTT TAATCAGCAG CAGCATTAAG AAGACATCCA CTGAGGCGGC AACCACAAAA 
CCTGCTGCAC CCCAGATCCC CGGCAAAATG GAGCTGCTGA GCAGCAACAC AGGAACAACA 
ACGACAACGA CGACAGCAAC GCACCACCTG CATCAGGCAA CCACCACTAA ACAGCTGTTG 
GTGCAGGATT ACCTCAGCTA CGCCTCACCA CCCACATATT CCCGCCTGCC GCCCGATGGT 
CATGAATTTC CACCCAATTT CAGCGAACCA CTGATCATGC ACAGCCACCC ACTAAAGGTC 
ACCACCGAGT TGAGTTACGA GATACAGAAA GGCGGAAAGG ATGAGTCCTC TGCACCACCG 
CTTCCCAAAA CGGGGCCACC AGCCACAGTT CCCAGGAAAG TATATCGCCA GGATTTGGTC 
ATCAACGTAG AGCCAGCACC CAGTTTAACC AGGGACTATC AGAGATCTCT GAGTGGTGGT 
ACTCCCCGGA AGCCTAGCGA TTGGCGCAAG GATGAAAAGT CGGAGAAATC AGTGCGCGAC 
AAGATTGCCA TGTTCTCGTC AAACAACGAA CTGGATGCCA TACCGCCAGC TCCGGCAACA 
GCCCCCATTT CAAGCTCCTT CACCCGGAAG CCCTTGAATA GGAGCAGTGA AAATCTTCTG 
GACAGTTGCT CATCATCCTC GGCTCCTTCG CTGAAAACTC GCGCGATGAG CGTGGAAAAT 
TTGAATGATG TTCAGCGGCA ATATCAGTTG GCCAAACAGC TGCCCCAGTT GCACGTGGCC 
GACTCTATGT ATTCCCTGAA CACAGCCACG CCCACACCGA GCTACGCCTC TTTGCCGAGA 
AGAAGCCACG GGGGATCTTA TTCTTCTGGA GTGGAGAGAA GGATCAGTTT CTCGGGAGAA 
GGCGGAGATG CGGCCAACCG AAAGGCAGCC ATTACAAATA TCCTAGAGCA AAGAAGAAGA 
AGTTTGTCGA AACTGCGAGG ATTGGTCATA CCAGAGAGAC CCCAACTGCT GGAACCCATC 
CTGGATCTGC CGGAGATCAA AAGCCAGGTA AAAGCAGCCA GCGGAGAGGA TAGTACGGAT 
AGCGGATTAG GCGAAAGCCA CCGCAGTACG GTGAATAGGA ATTGCCAATT GGGTGCAGGA 
GGTGCCGCAA ATAACTATCG CAGCATTTTA AATACCAATC AAAGAAGACC CCTGGAGCAA 
CAGCTCTCTC AACCACCAGC CAAACCACCC AGGACATCGT TGACACCACT TCAGCCGAGA 
AGCATGATGA TACCACCACC ACCACCTCCT TTAGATCAGG AAAGTGATAC GGATTCGGTG 
TTTTCGCACA CTGCTAGGGT TGCCACTCCG CCGGAGAAGT TTGCACTAAC CAGAACCCTC 
AGCTCCGAAA CAAACACTTC GATAGCTAGT TCAAACACCT CTACTCTAAC ATCAGGATCT 
TCTGCCGGAT CTCAAGCCAG TTGCAGTTCG CTGGGCAGCA CACCAGCGGT GGATCTAACC 
AGGCGGGTTC TCAAGAGCCA GGTGATCAAT GGCGAAGCCG TTGCGCTCTC CAGTCGCAAA 
AGTATTCTGG CCTCGGCCAA ATGTCGCAGT GCCAAGAGTC GAGGTCAAGA GGAGGATAAC 
GACAGCACCG ATGGTGAAGC CTGTTCCCTG GCCAATCGTA GAATGAAGCC GATTTCCAGC 
TATAAGCTCC AGCAGCAACA GATCCAACTG GGCAAACAAC TGGTAGTGGA TAAGCTGATC 
AATGTGGCCG CTTATGTGGA ACTAACCTCG GATACGGACG ACAGCAGCCG TAGATCTGAT 
ACGCCTGCCA AGATCAGTGC CATGTTCATA GACGAGGAGC GCAAGGCCAG CTTCAAAGGA 
GATCCTAACC AGCAGGCTAA GGTCAAAGTG GAGCAGGTCA AACCTATGGT CCTCCCAATG 
CTGCTACCAT CTGCGAAAAG GGAACCGCTG AAGCAACAGA CCACCGCCGA ACTGCGCGAA 
AAGTTCGAAA GGAGTGCTGC AGCCCAAGCT CAAACCCAAA ACCACTCGCC CGTAATCCAT 
AAGGTCACCC AGAAGCCGCA TCATGAGCGA TTCTCCTCGC TGGACTCCCT GGCGTCCAGT 
TCCTCCGGCG TTAGTTCCAC CACCCAAAAC GTGAGCACCA CCCAGGAAAC GGCCACCGAA 
TTCGGCAGCT TTTCCTCGCT GGGTAGCAAC CAGAGTTTGA TCACCGCCCA GGATGTCCAG 
CAGATAGTCG AGGAGGCTGA TCCTCCACTA AAGACCCCAG AAGCGTTCAT CATCGTCCTG 
CAGAGGGAAA ATCCCGAGAG CAGCATCGGT ATCACTTTGG CCGGCGGTTC TGATTATGAG 
GCTAAGGAAA TTACGATCCA TAAGATCCTG AGCAACACGC CAGCTGCCAA GGATGGACGT 
CTAAAGAAGG GTGATCGAAT CCTTGCCGTC AATGGAATGA GCATGCGCGG ACTGACGCAC 
CGCGAATCCA TCAGTGTGCT TAAGACCCCC CGACCTGAGG TCGTGCTAGT GGTGACCCGC 
TCCGAGTCGC TGGTGGTGAA GGCGCTGACC AAGAAACGAT CCTCCCTGGG ATCACTTAGC 
TCGCTCAATG AGAAACCCAC GGAGCTGGAC TATGAACGCA AGAGGAACTA CCACAAGGCC 
TCCCGATCTC TGGACTTGGA TCTTGATCTG GTGTCCAATG AGGCTGGTGA ATCGCCAGTG 
GCTACTACTC CCAGCACGGG ATCCGTAAGT CCACCGCAGC CGGCGAGCTT GCACGACGAG 
GATGCGGAGG CCACCATCGC AGGTATCCGG GCCAGGAGGC AGTTGTCCCG CGGAGATGCC 
GCCAAGCTTA GCACGAGCGA GCTCCTGGAA CGGGCGGCAG AGGCCAGAAA TGCCATCGCA 
GCTGAGATTC GTGCACAAGC GGAGGATGCG GCGGCCAGTG GAGGTGGAGC TCGTTGTGTG 
GAGATTGTTA AGGACAGCTG TGGACTGGGT TTCTCCATCG AAGGAGGCTT TGATTCGCCG 
CTGGGAAATC GTCCCCTTAT TGTCAAAAAG GTGTTCATGG GTGGTGCCGC CCAGAAGACC 
AACCAGGTGC GCAATGGCGA CGAAATCCTT AGCATCAATG GTGCCTCCAC GTCGCGGATG 
ACACGTGTGG ATGCCTGGAA CTATATGAAG CAACTGCCGC TGGGACCGGT CAAGATTTGT 
TTCGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137953.1
CDS	98..5626
Translation	MEVNAGGLAQLTDDKIADHDASATRADTRDEVTTNQTQEISSSSSSSVKSQKTTTTTRVTTTKTSSSSSSSSCSSTVIPDVAFKLQPYEERDDILSEPITTIHEERRVLSEQKTLSESRTRDALTGEEQLVTSANSKSSSARFKKISSNDNLLDLGDEQDMQEQPLTATITQESSLRERLECPQDNQSIDRGTLTLSERGKQLSEKEGLTTYTECETSEKESYLEGQQVLNGKGGFVSQDENSDQPTYEREVSETLTIVNDGEKQVTKKLEQNKEIAGKKGAKLLKKTDEERRLEQEAQKLIESYQKVKKEAEKLYKLELADDDQGFDLSAFEQAEEEDKPEVSEEIKEVKDSQPVKVDIQIEEEVKTPNQVIGEVKVEDVNKEIKETVQVSQQEIKKEVKVSHQETKETFKSEEKDESNELKIVKEEVKVTHQDLREQIQEIEAVKVVHKEVKVIKESAKVTTTTSHKQATKEVIISKEDHKNDSALPAVSTTKVEIISPPLEDDLGYVLHKHIIFPQEKVKTAPTPLPKPKPKPPVPTNKPKMPVNLTKPKSAPPPVPSKRSELSGSGGKPTPTQRRGSLETAQPPKPLERIIVGVEQRDEPEKPQPIINLASVDLPNVAPNSKQATVETTPVVEMPPDELQFESHQLPDDGEKEKDLHQESNDLLSGSSSSSVTTPITTPILVSATSSMDSVQSVIEVVNGMPIISNQQTDDDDIDEVAAYDDGMHLEAEGTKDVRPVQEVTTLTLDREHESDLGTLSNHHSNISSISGINASSNIPAATLISSSIKKTSTEAATTKPAAPQIPGKMELLSSNTGTTTTTTTATHHLHQATTTKQLLVQDYLSYASPPTYSRLPPDGHEFPPNFSEPLIMHSHPLKVTTELSYEIQKGGKDESSAPPLPKTGPPATVPRKVYRQDLVINVEPAPSLTRDYQRSLSGGTPRKPSDWRKDEKSEKSVRDKIAMFSSNNELDAIPPAPATAPISSSFTRKPLNRSSENLLDSCSSSSAPSLKTRAMSVENLNDVQRQYQLAKQLPQLHVADSMYSLNTATPTPSYASLPRRSHGGSYSSGVERRISFSGEGGDAANRKAAITNILEQRRRSLSKLRGLVIPERPQLLEPILDLPEIKSQVKAASGEDSTDSGLGESHRSTVNRNCQLGAGGAANNYRSILNTNQRRPLEQQLSQPPAKPPRTSLTPLQPRSMMIPPPPPPLDQESDTDSVFSHTARVATPPEKFALTRTLSSETNTSIASSNTSTLTSGSSAGSQASCSSLGSTPAVDLTRRVLKSQVINGEAVALSSRKSILASAKCRSAKSRGQEEDNDSTDGEACSLANRRMKPISSYKLQQQQIQLGKQLVVDKLINVAAYVELTSDTDDSSRRSDTPAKISAMFIDEERKASFKGDPNQQAKVKVEQVKPMVLPMLLPSAKREPLKQQTTAELREKFERSAAAQAQTQNHSPVIHKVTQKPHHERFSSLDSLASSSSGVSSTTQNVSTTQETATEFGSFSSLGSNQSLITAQDVQQIVEEADPPLKTPEAFIIVLQRENPESSIGITLAGGSDYEAKEITIHKILSNTPAAKDGRLKKGDRILAVNGMSMRGLTHRESISVLKTPRPEVVLVVTRSESLVVKALTKKRSSLGSLSSLNEKPTELDYERKRNYHKASRSLDLDLDLVSNEAGESPVATTPSTGSVSPPQPASLHDEDAEATIAGIRARRQLSRGDAAKLSTSELLERAAEARNAIAAEIRAQAEDAAASGGGARCVEIVKDSCGLGFSIEGGFDSPLGNRPLIVKKVFMGGAAQKTNQVRNGDEILSINGASTSRMTRVDAWNYMKQLPLGPVKICFA

Target ORF information:

RefSeq Version	NM_001144481.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster big bang (bbg), transcript variant J, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144481.2

ORF Insert Sequence:

ATGGAAGTAA ACGCCGGTGG ACTGGCCCAG CTGACTGATG ATAAGATTGC TGACCATGAC 
GCAAGTGCAA CCAGAGCGGA TACTCGCGAC GAAGTCACAA CAAACCAAAC CCAGGAAATC 
AGCTCCAGCA GTTCGAGTTC TGTTAAAAGT CAAAAGACCA CCACAACCAC TCGAGTGACC 
ACCACCAAGA CTAGTTCCAG TTCCAGTTCC TCCAGTTGCT CATCCACTGT TATCCCAGAT 
GTTGCCTTCA AGCTGCAACC CTATGAGGAA AGGGACGATA TACTCTCGGA ACCCATTACC 
ACCATCCACG AAGAACGACG TGTGCTAAGT GAGCAGAAGA CCCTCAGTGA ATCGAGGACT 
CGCGATGCAT TAACCGGCGA AGAGCAGCTG GTAACTAGTG CCAATTCGAA GTCGAGCAGT 
GCCCGCTTCA AGAAGATCAG CAGCAACGAC AACCTACTCG ACCTTGGCGA CGAGCAGGAC 
ATGCAGGAGC AGCCGTTGAC CGCCACGATC ACCCAGGAGT CGAGTCTGAG GGAGCGGTTG 
GAGTGTCCTC AGGATAACCA GAGTATCGAC AGGGGTACTC TGACCCTATC CGAGCGGGGA 
AAGCAGCTGA GCGAGAAAGA AGGCCTCACC ACCTACACGG AATGTGAGAC GAGTGAAAAG 
GAGTCCTACC TAGAGGGCCA ACAAGTCCTC AACGGCAAAG GCGGTTTCGT TAGCCAGGAT 
GAGAATAGTG ACCAACCAAC TTACGAACGA GAGGTCAGCG AGACACTAAC GATCGTCAAC 
GATGGCGAAA AGCAGGTCAC CAAGAAATTG GAACAGAACA AGGAAATTGC TGGCAAAAAG 
GGTGCCAAGC TCCTTAAGAA GACCGATGAG GAGCGACGTC TTGAGCAGGA GGCCCAAAAG 
CTGATCGAAA GCTATCAGAA AGTCAAGAAA GAAGCGGAGA AGCTGTACAA GCTGGAATTG 
GCCGACGATG ATCAGGGCTT TGATCTGAGT GCCTTCGAGC AGGCGGAGGA AGAGGATAAG 
CCAGAAGTTT CTGAGGAAAT CAAAGAAGTG AAGGATTCTC AGCCAGTAAA GGTCGATATT 
CAAATTGAAG AAGAGGTTAA AACACCTAAT CAAGTCATAG GGGAAGTCAA AGTGGAAGAT 
GTGAATAAGG AAATTAAAGA GACGGTACAA GTCTCTCAAC AGGAAATTAA GAAAGAGGTC 
AAGGTCTCAC ACCAAGAAAC AAAAGAAACC TTTAAGAGTG AAGAAAAAGA TGAGAGTAAT 
GAACTCAAAA TAGTTAAGGA GGAGGTCAAA GTAACACATC AGGATCTTAG GGAACAGATC 
CAGGAGATCG AGGCTGTCAA AGTGGTGCAC AAAGAAGTAA AAGTTATTAA GGAAAGTGCT 
AAAGTCACTA CGACCACGAG TCACAAGCAA GCAACTAAAG AAGTAATCAT CTCGAAAGAA 
GATCACAAAA ATGATAGCGC ACTCCCTGCC GTGTCAACCA CAAAAGTAGA GATCATCTCA 
CCTCCCCTGG AAGACGACTT GGGTTATGTC CTCCACAAGC ACATTATTTT TCCCCAGGAA 
AAGGTCAAGA CGGCTCCTAC GCCACTGCCA AAACCGAAAC CCAAACCTCC AGTGCCCACG 
AACAAACCCA AAATGCCGGT CAATTTGACC AAACCGAAAT CAGCACCACC ACCGGTGCCC 
AGTAAGAGGA GTGAGCTATC CGGATCCGGT GGCAAGCCCA CACCCACCCA AAGACGCGGC 
AGCCTGGAGA CCGCCCAGCC TCCAAAACCA CTAGAACGTA TAATTGTCGG TGTGGAGCAG 
CGGGATGAGC CAGAAAAGCC CCAGCCAATT ATCAACTTGG CCAGCGTGGA TCTGCCAAAT 
GTGGCGCCCA ACAGCAAGCA AGCGACAGTG GAAACGACTC CCGTGGTCGA AATGCCACCG 
GATGAGCTCC AATTCGAGAG TCACCAACTG CCGGATGACG GTGAAAAGGA AAAGGACTTG 
CACCAGGAGT CAAATGACCT GCTCAGCGGG TCGTCATCGT CGTCGGTAAC CACGCCCATA 
ACAACGCCAA TTTTGGTATC TGCCACCTCG TCGATGGACT CGGTGCAAAG TGTCATCGAG 
GTCGTCAATG GCATGCCCAT CATCAGCAAT CAGCAAACTG ACGACGACGA CATTGATGAA 
GTTGCAGCGT ATGACGATGG GATGCATTTG GAGGCCGAAG GTACGAAGGA TGTTCGTCCC 
GTCCAGGAGG TGACAACGTT GACGCTCGAC AGGGAACACG AATCAGATCT TGGTACCCTC 
AGTAACCACC ACAGCAATAT AAGCAGCATT AGCGGCATTA ACGCCAGTAG CAACATCCCG 
GCAGCAACTT TAATCAGCAG CAGCATTAAG AAGACATCCA CTGAGGCGGC AACCACAAAA 
CCTGCTGCAC CCCAGATCCC CGGCAAAATG GAGCTGCTGA GCAGCAACAC AGGAACAACA 
ACGACAACGA CGACAGCAAC GCACCACCTG CATCAGGCAA CCACCACTAA ACAGCTGTTG 
GTGCAGGATT ACCTCAGCTA CGCCTCACCA CCCACATATT CCCGCCTGCC GCCCGATGGT 
CATGAATTTC CACCCAATTT CAGCGAACCA CTGATCATGC ACAGCCACCC ACTAAAGGTC 
ACCACCGAGT TGAGTTACGA GATACAGAAA GGCGGAAAGG ATGAGTCCTC TGCACCACCG 
CTTCCCAAAA CGGGGCCACC AGCCACAGTT CCCAGGAAAG TATATCGCCA GGATTTGGTC 
ATCAACGTAG AGCCAGCACC CAGTTTAACC AGGGACTATC AGAGATCTCT GAGTGGTGGT 
ACTCCCCGGA AGCCTAGCGA TTGGCGCAAG GATGAAAAGT CGGAGAAATC AGTGCGCGAC 
AAGATTGCCA TGTTCTCGTC AAACAACGAA CTGGATGCCA TACCGCCAGC TCCGGCAACA 
GCCCCCATTT CAAGCTCCTT CACCCGGAAG CCCTTGAATA GGAGCAGTGA AAATCTTCTG 
GACAGTTGCT CATCATCCTC GGCTCCTTCG CTGAAAACTC GCGCGATGAG CGTGGAAAAT 
TTGAATGATG TTCAGCGGCA ATATCAGTTG GCCAAACAGC TGCCCCAGTT GCACGTGGCC 
GACTCTATGT ATTCCCTGAA CACAGCCACG CCCACACCGA GCTACGCCTC TTTGCCGAGA 
AGAAGCCACG GGGGATCTTA TTCTTCTGGA GTGGAGAGAA GGATCAGTTT CTCGGGAGAA 
GGCGGAGATG CGGCCAACCG AAAGGCAGCC ATTACAAATA TCCTAGAGCA AAGAAGAAGA 
AGTTTGTCGA AACTGCGAGG ATTGGTCATA CCAGAGAGAC CCCAACTGCT GGAACCCATC 
CTGGATCTGC CGGAGATCAA AAGCCAGGTA AAAGCAGCCA GCGGAGAGGA TAGTACGGAT 
AGCGGATTAG GCGAAAGCCA CCGCAGTACG GTGAATAGGA ATTGCCAATT GGGTGCAGGA 
GGTGCCGCAA ATAACTATCG CAGCATTTTA AATACCAATC AAAGAAGACC CCTGGAGCAA 
CAGCTCTCTC AACCACCAGC CAAACCACCC AGGACATCGT TGACACCACT TCAGCCGAGA 
AGCATGATGA TACCACCACC ACCACCTCCT TTAGATCAGG AAAGTGATAC GGATTCGGTG 
TTTTCGCACA CTGCTAGGGT TGCCACTCCG CCGGAGAAGT TTGCACTAAC CAGAACCCTC 
AGCTCCGAAA CAAACACTTC GATAGCTAGT TCAAACACCT CTACTCTAAC ATCAGGATCT 
TCTGCCGGAT CTCAAGCCAG TTGCAGTTCG CTGGGCAGCA CACCAGCGGT GGATCTAACC 
AGGCGGGTTC TCAAGAGCCA GGTGATCAAT GGCGAAGCCG TTGCGCTCTC CAGTCGCAAA 
AGTATTCTGG CCTCGGCCAA ATGTCGCAGT GCCAAGAGTC GAGGTCAAGA GGAGGATAAC 
GACAGCACCG ATGGTGAAGC CTGTTCCCTG GCCAATCGTA GAATGAAGCC GATTTCCAGC 
TATAAGCTCC AGCAGCAACA GATCCAACTG GGCAAACAAC TGGTAGTGGA TAAGCTGATC 
AATGTGGCCG CTTATGTGGA ACTAACCTCG GATACGGACG ACAGCAGCCG TAGATCTGAT 
ACGCCTGCCA AGATCAGTGC CATGTTCATA GACGAGGAGC GCAAGGCCAG CTTCAAAGGA 
GATCCTAACC AGCAGGCTAA GGTCAAAGTG GAGCAGGTCA AACCTATGGT CCTCCCAATG 
CTGCTACCAT CTGCGAAAAG GGAACCGCTG AAGCAACAGA CCACCGCCGA ACTGCGCGAA 
AAGTTCGAAA GGAGTGCTGC AGCCCAAGCT CAAACCCAAA ACCACTCGCC CGTAATCCAT 
AAGGTCACCC AGAAGCCGCA TCATGAGCGA TTCTCCTCGC TGGACTCCCT GGCGTCCAGT 
TCCTCCGGCG TTAGTTCCAC CACCCAAAAC GTGAGCACCA CCCAGGAAAC GGCCACCGAA 
TTCGGCAGCT TTTCCTCGCT GGGTAGCAAC CAGAGTTTGA TCACCGCCCA GGATGTCCAG 
CAGATAGTCG AGGAGGCTGA TCCTCCACTA AAGACCCCAG AAGCGTTCAT CATCGTCCTG 
CAGAGGGAAA ATCCCGAGAG CAGCATCGGT ATCACTTTGG CCGGCGGTTC TGATTATGAG 
GCTAAGGAAA TTACGATCCA TAAGATCCTG AGCAACACGC CAGCTGCCAA GGATGGACGT 
CTAAAGAAGG GTGATCGAAT CCTTGCCGTC AATGGAATGA GCATGCGCGG ACTGACGCAC 
CGCGAATCCA TCAGTGTGCT TAAGACCCCC CGACCTGAGG TCGTGCTAGT GGTGACCCGC 
TCCGAGTCGC TGGTGGTGAA GGCGCTGACC AAGAAACGAT CCTCCCTGGG ATCACTTAGC 
TCGCTCAATG AGAAACCCAC GGAGCTGGAC TATGAACGCA AGAGGAACTA CCACAAGGCC 
TCCCGATCTC TGGACTTGGA TCTTGATCTG GTGTCCAATG AGGCTGGTGA ATCGCCAGTG 
GCTACTACTC CCAGCACGGG ATCCGTAAGT CCACCGCAGC CGGCGAGCTT GCACGACGAG 
GATGCGGAGG CCACCATCGC AGGTATCCGG GCCAGGAGGC AGTTGTCCCG CGGAGATGCC 
GCCAAGCTTA GCACGAGCGA GCTCCTGGAA CGGGCGGCAG AGGCCAGAAA TGCCATCGCA 
GCTGAGATTC GTGCACAAGC GGAGGATGCG GCGGCCAGTG GAGGTGGAGC TCGTTGTGTG 
GAGATTGTTA AGGACAGCTG TGGACTGGGT TTCTCCATCG AAGGAGGCTT TGATTCGCCG 
CTGGGAAATC GTCCCCTTAT TGTCAAAAAG GTGTTCATGG GTGGTGCCGC CCAGAAGACC 
AACCAGGTGC GCAATGGCGA CGAAATCCTT AGCATCAATG GTGCCTCCAC GTCGCGGATG 
ACACGTGTGG ATGCCTGGAA CTATATGAAG CAACTGCCGC TGGGACCGGT CAAGATTTGT 
TTCGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bbg ( NM_001259826.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246755.1 Drosophila melanogaster big bang (bbg), transcript variant K, mRNA.

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CloneID	OFa18500
Clone ID Related Accession (Same CDS sequence)	NM_001259826.1 , NM_140448.3
Accession Version	NM_001259826.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	big bang, isoform K
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCTCCA CACCAAAAGC GAATGGCACG ACAGCGAAGA CGGCAACCAT AAATGATGAC 
TACATAACGG TCGTGGAAGT GGATGATCCG ACGTCGAACC CGAAGGATCG CCTTAAGAAG 
CTAGTCAAGC GTCAAAGCTC TGTGGCGGAG GACTCCTCGT TCATCACAGT GCTGACGATT 
AACGAGATGG AGTTGCTTCA ACAGCGAAAA AAGGAGGAGG ATGTTGGTAG TGGCTCAAGC 
CCTACGATGG AGGAGCAAAT AGAGAATGTC ACAGTGTTCA GATTACCGGG AGAACGTCTC 
GGTTTTGGCC TAAAGTTTCA AGGAGGCACG AGGAGCACAG AGCTCGTCCA AAAACTGTAC 
ATACAATCCT GTGCTGCAGA CAGTCCTGCC TCCAAAGTGT CCACCAGCTG GGGTAATCTA 
CGAGAGGGTG ATGAGATCGT CAGTATCGAT GGTCGTGATG TCCGGGAATT GACTCGAATC 
GATTGCGTTC GCGGCCTCAA AGACAATGTG GCCATTAAGC TGGTGGTGCG AAATGGTCAC 
GGACAGAAGC CTCCCAGCGA AGAGGACCCA CAGCAGCAGG AACACCTTTC GATCACACTG 
AATGCCCAAC CACCGCCTCC GCCACCAGTG CCGCCAAGAA AGTTGGTGAG ACGGCAAAAC 
TCCAAAGAGA ACCAGGCTCA GTTGCTCATT GAGAAGCCAT TGACGCCACC ACCGGATGCG 
GAGTACTACC TGAATCTTTT AACCGAATCC ATTAAGGCGG GCTCAGAGTC GGATGATACC 
GCCAGCACAA TTTCCACTGT TATCGACAAG TTTTCCGTGG GCTCCAATTA CTCCTCGGAC 
AGTAGTCTCA ATGGCCACGA GCTGGCCAAG GTTTTGCAAC CTTTTACCCT TCTCGAGCAG 
GAGTTTCTGC CTTTGGAGCA GCCGCTAGGA CATCCCAAGT TGCTTATTCC TGGCAATAAC 
TATGAAAATG TGGAGTTCAA AACGGAGAAG GTCAATGTAT ACGAAAATGT GGAGCTCAGG 
AGTCCGGAAA CGACGCCGAC ACCAAAACCC AGAGTCCAAT TGGCCACCGT GGAGCCAAAG 
AAGCGTTCGA TCATACCCAT GCCACGAAAA ATTCCTACTC CAACCAAGCT GCCCATTGAG 
GTGACCCCAC CCCGGGTTCC CATATTGGAG GAACCCAAAA CGCCCACAAA TAACAAAGTT 
GATTCTCCCA AAACACCAAC AAACGAACTC AAGGAGTCTC CCATCAGTGC TACAAAAATA 
CCTAAGGCCA AGTTCTCGAG GGCCAAAACT GAAGGAGAGA TCAAGTTGCA TTTGCCAGCC 
CCAAAACAGC AATCACCACA AAGTAAATCT CGTATACCCA TAGTCAGTAC CCCTACAGCA 
CAAAAAACTG TTCCCAAACC AGTATCACCC ACAATGAATG GTACACCACT CAGTTCAAAT 
ATACCACGGC TGTTGCAGAA GCAGAAATCC GAAACGGATC TGAAATTGAA CTTGTATAGG 
AGTAAATCTA AGGAGTCGAG TCCCAGACCT CCCTTGCAAA GAGCGAACTC GGCAGAGGCT 
CCAGAAAGGA CCTTTATCCC TGTACTTCTT AATGGGAACT CCAAGAGCTC AAACTCCTTG 
GAGAGCGTCA GTTCGATAGG AAGTAGTAGC AGTGGAAATA GTGTAAGATC ACCAAAAGGA 
CCAAAGCCCA AGCCACCAGA GCGAGTTCAA TCGCTTCAGA AAACCCAAAT ACCCAAACTG 
CAGGCACTCC CAACTACTCC ACCTCAACAG ATACCCAAGT TGAGTATGCA GACGTTCAAG 
CAGAGCCCTC CCACACCCCG ATCAACTCCA GTGGGAACTC CATCTCCAAC AAGTAATCGA 
GAGATACGCT TCAAGATTCA GACGTACGAA AGCAAAACCC AGGACGAAGA TAAACTACCC 
AGCTTATTTG ACCTAGTGCA CAGTCAGGAG AATGATAAGG ATAAAGATAG GGATAGTGAT 
TCGCTCAAGA CCCACAATAG CATCACTGCT CTTTTGGCAG CCGCCGCTGC CGAGGCCGCT 
GATCTCTCTC GGGAGTCGCC ACCTCCGTTG GTCGTTGGCA AATGCATGAA GATCGTCGAT 
GACACCAATA CCACCACCTG CTATACCAGT TCCAGCAGCG GGGAGGAGGA CGAAGACGAT 
CTGGATGCCT CCAACCGGGA GTACATATGC GAGGACGGCG AGAAGTTGGG ACCTCCGGAG 
CTGATAAATG GCCCCGGTCC CTCGGAGGCC TACTTCAACA TGTTCTGGCA CTCGAACATG 
CTGCCCACAA TCGGCGAGGT TGAAGAGGAG TTCTCCTCCC TGGAACCCCA ATCCCTAACA 
AACGGAAACC TTGTCAAATC GGAGGAGTCG AAGAAGTTAC CCAAAATGGA AGTAAACGCC 
GGTGGACTGG CCCAGCTGAC TGATGATAAG ATTGCTGACC ATGACGCAAG TGCAACCAGA 
GCGGATACTC GCGACGAAGT CACAACAAAC CAAACCCAGG AAATCAGCTC CAGCAGTTCG 
AGTTCTGTTA AAAGTCAAAA GACCACCACA ACCACTCGAG TGACCACCAC CAAGACTAGT 
TCCAGTTCCA GTTCCTCCAG TTGCTCATCC ACTGTTATCC CAGATGTTGC CTTCAAGCTG 
CAACCCTATG AGGAAAGGGA CGATATACTC TCGGAACCCA TTACCACCAT CCACGAAGAA 
CGACGTGTGC TAAGTGAGCA GAAGACCCTC AGTGAATCGA GGACTCGCGA TGCATTAACC 
GGCGAAGAGC AGCTGGTAAC TAGTGCCAAT TCGAAGTCGA GCAGTGCCCG CTTCAAGAAG 
ATCAGCAGCA ACGACAACCT ACTCGACCTT GGCGACGAGC AGGACATGCA GGAGCAGCCG 
TTGACCGCCA CGATCACCCA GGAGTCGAGT CTGAGGGAGC GGTTGGAGTG TCCTCAGGAT 
AACCAGAGTA TCGACAGGGG TACTCTGACC CTATCCGAGC GGGGAAAGCA GCTGAGCGAG 
AAAGAAGGCC TCACCACCTA CACGGAATGT GAGACGAGTG AAAAGGAGTC CTACCTAGAG 
GGCCAACAAG TCCTCAACGG CAAAGGCGGT TTCGTTAGCC AGGATGAGAA TAGTGACCAA 
CCAACTTACG AACGAGAGGT CAGCGAGACA CTAACGATCG TCAACGATGG CGAAAAGCAG 
GTCACCAAGA AATTGGAACA GAACAAGGAA ATTGCTGGCA AAAAGGGTGC CAAGCTCCTT 
AAGAAGACCG ATGAGGAGCG ACGTCTTGAG CAGGAGGCCC AAAAGCTGAT CGAAAGCTAT 
CAGAAAGTCA AGAAAGAAGC GGAGAAGCTG TACAAGCTGG AATTGGCCGA CGATGATCAG 
GGCTTTGATC TGAGTGCCTT CGAGCAGGCG GAGGAAGAGG ATAAGCCAGA AGTTTCTGAG 
GAAATCAAAG AAGTGAAGGA TTCTCAGCCA GTAAAGGTCG ATATTCAAAT TGAAGAAGAG 
GTTAAAACAC CTAATCAAGT CATAGGGGAA GTCAAAGTGG AAGATGTGAA TAAGGAAATT 
AAAGAGACGG TACAAGTCTC TCAACAGGAA ATTAAGAAAG AGGTCAAGGT CTCACACCAA 
GAAACAAAAG AAACCTTTAA GAGTGAAGAA AAAGATGAGA GTAATGAACT CAAAATAGTT 
AAGGAGGAGG TCAAAGTAAC ACATCAGGAT CTTAGGGAAC AGATCCAGGA GATCGAGGCT 
GTCAAAGTGG TGCACAAAGA AGTAAAAGTT ATTAAGGAAA GTGCTAAAGT CACTACGACC 
ACGAGTCACA AGCAAGCAAC TAAAGAAGTA ATCATCTCGA AAGAAGATCA CAAAAATGAT 
AGCGCACTCC CTGCCGTGTC AACCACAAAA GTAGAGATCA TCTCACCTCC CCTGGAAGAC 
GACTTGGGTT ATGTCCTCCA CAAGCACATT ATTTTTCCCC AGGAAAAGGT CAAGACGGCT 
CCTACGCCAC TGCCAAAACC GAAACCCAAA CCTCCAGTGC CCACGAACAA ACCCAAAATG 
CCGGTCAATT TGACCAAACC GAAATCAGCA CCACCACCGG TGCCCAGTAA GAGGAGTGAG 
CTATCCGGAT CCGGTGGCAA GCCCACACCC ACCCAAAGAC GCGGCAGCCT GGAGACCGCC 
CAGCCTCCAA AACCACTAGA ACGTATAATT GTCGGTGTGG AGCAGCGGGA TGAGCCAGAA 
AAGCCCCAGC CAATTATCAA CTTGGCCAGC GTGGATCTGC CAAATGTGGC GCCCAACAGC 
AAGCAAGCGA CAGTGGAAAC GACTCCCGTG GTCGAAATGC CACCGGATGA GCTCCAATTC 
GAGAGTCACC AACTGCCGGA TGACGGTGAA AAGGAAAAGG ACTTGCACCA GGAGTCAAAT 
GACCTGCTCA GCGGGTCGTC ATCGTCGTCG GTAACCACGC CCATAACAAC GCCAATTTTG 
GTATCTGCCA CCTCGTCGAT GGACTCGGTG CAAAGTGTCA TCGAGGTCGT CAATGGCATG 
CCCATCATCA GCAATCAGCA AACTGACGAC GACGACATTG ATGAAGTTGC AGCGTATGAC 
GATGGGATGC ATTTGGAGGC CGAAGGTACG AAGGATGTTC GTCCCGTCCA GGAGGTGACA 
ACGTTGACGC TCGACAGGGA ACACGAATCA GATCTTGGTA CCCTCAGTAA CCACCACAGC 
AATATAAGCA GCATTAGCGG CATTAACGCC AGTAGCAACA TCCCGGCAGC AACTTTAATC 
AGCAGCAGCA TTAAGAAGAC ATCCACTGAG GCGGCAACCA CAAAACCTGC TGCACCCCAG 
ATCCCCGGCA AAATGGAGCT GCTGAGCAGC AACACAGGAA CAACAACGAC AACGACGACA 
GCAACGCACC ACCTGCATCA GGCAACCACC ACTAAACAGC TGTTGGTGCA GGATTACCTC 
AGCTACGCCT CACCACCCAC ATATTCCCGC CTGCCGCCCG ATGGTCATGA ATTTCCACCC 
AATTTCAGCG AACCACTGAT CATGCACAGC CACCCACTAA AGGTCACCAC CGAGTTGAGT 
TACGAGATAC AGAAAGGCGG AAAGGATGAG TCCTCTGCAC CACCGCTTCC CAAAACGGGG 
CCACCAGCCA CAGTTCCCAG GAAAGTATAT CGCCAGGATT TGGTCATCAA CGTAGAGCCA 
GCACCCAGTT TAACCAGGGA CTATCAGAGA TCTCTGAGTG GTGGTACTCC CCGGAAGCCT 
AGCGATTGGC GCAAGGATGA AAAGTCGGAG AAATCAGTGC GCGACAAGAT TGCCATGTTC 
TCGTCAAACA ACGAACTGGA TGCCATACCG CCAGCTCCGG CAACAGCCCC CATTTCAAGC 
TCCTTCACCC GGAAGCCCTT GAATAGGAGC AGTGAAAATC TTCTGGACAG TTGCTCATCA 
TCCTCGGCTC CTTCGCTGAA AACTCGCGCG ATGAGCGTGG AAAATTTGAA TGATGTTCAG 
CGGCAATATC AGTTGGCCAA ACAGCTGCCC CAGTTGCACG TGGCCGACTC TATGTATTCC 
CTGAACACAG CCACGCCCAC ACCGAGCTAC GCCTCTTTGC CGAGAAGAAG CCACGGGGGA 
TCTTATTCTT CTGGAGTGGA GAGAAGGATC AGTTTCTCGG GAGAAGGCGG AGATGCGGCC 
AACCGAAAGG CAGCCATTAC AAATATCCTA GAGCAAAGAA GAAGAAGTTT GTCGAAACTG 
CGAGGATTGG TCATACCAGA GAGACCCCAA CTGCTGGAAC CCATCCTGGA TCTGCCGGAG 
ATCAAAAGCC AGGTAAAAGC AGCCAGCGGA GAGGATAGTA CGGATAGCGG ATTAGGCGAA 
AGCCACCGCA GTACGGTGAA TAGGAATTGC CAATTGGGTG CAGGAGGTGC CGCAAATAAC 
TATCGCAGCA TTTTAAATAC CAATCAAAGA AGACCCCTGG AGCAACAGCT CTCTCAACCA 
CCAGCCAAAC CACCCAGGAC ATCGTTGACA CCACTTCAGC CGAGAAGCAT GATGATACCA 
CCACCACCAC CTCCTTTAGA TCAGGAAAGT GATACGGATT CGGTGTTTTC GCACACTGCT 
AGGGTTGCCA CTCCGCCGGA GAAGTTTGCA CTAACCAGAA CCCTCAGCTC CGAAACAAAC 
ACTTCGATAG CTAGTTCAAA CACCTCTACT CTAACATCAG GATCTTCTGC CGGATCTCAA 
GCCAGTTGCA GTTCGCTGGG CAGCACACCA GCGGTGGATC TAACCAGGCG GGTTCTCAAG 
AGCCAGGTGA TCAATGGCGA AGCCGTTGCG CTCTCCAGTC GCAAAAGTAT TCTGGCCTCG 
GCCAAATGTC GCAGTGCCAA GAGTCGAGGT CAAGAGGAGG ATAACGACAG CACCGATGGT 
GAAGCCTGTT CCCTGGCCAA TCGTAGAATG AAGCCGATTT CCAGCTATAA GCTCCAGCAG 
CAACAGATCC AACTGGGCAA ACAACTGGTA GTGGATAAGC TGATCAATGT GGCCGCTTAT 
GTGGAACTAA CCTCGGATAC GGACGACAGC AGCCGTAGAT CTGATACGCC TGCCAAGATC 
AGTGCCATGT TCATAGACGA GGAGCGCAAG GCCAGCTTCA AAGGAGATCC TAACCAGCAG 
GCTAAGGTCA AAGTGGAGCA GGTCAAACCT ATGGTCCTCC CAATGCTGCT ACCATCTGCG 
AAAAGGGAAC CGCTGAAGCA ACAGACCACC GCCGAACTGC GCGAAAAGTT CGAAAGGAGT 
GCTGCAGCCC AAGCTCAAAC CCAAAACCAC TCGCCCGTAA TCCATAAGGT CACCCAGAAG 
CCGCATCATG AGCGATTCTC CTCGCTGGAC TCCCTGGCGT CCAGTTCCTC CGGCGTTAGT 
TCCACCACCC AAAACGTGAG CACCACCCAG GAAACGGCCA CCGAATTCGG CAGCTTTTCC 
TCGCTGGGTA GCAACCAGAG TTTGATCACC GCCCAGGATG TCCAGCAGAT AGTCGAGGAG 
GCTGATCCTC CACTAAAGAC CCCAGAAGCG TTCATCATCG TCCTGCAGAG GGAAAATCCC 
GAGAGCAGCA TCGGTATCAC TTTGGCCGGC GGTTCTGATT ATGAGGCTAA GGAAATTACG 
ATCCATAAGA TCCTGAGCAA CACGCCAGCT GCCAAGGATG GACGTCTAAA GAAGGGTGAT 
CGAATCCTTG CCGTCAATGG AATGAGCATG CGCGGACTGA CGCACCGCGA ATCCATCAGT 
GTGCTTAAGA CCCCCCGACC TGAGGTCGTG CTAGTGGTGA CCCGCTCCGA GTCGCTGGTG 
GTGAAGGCGC TGACCAAGAA ACGATCCTCC CTGGGATCAC TTAGCTCGCT CAATGAGAAA 
CCCACGGAGC TGGACTATGA ACGCAAGAGG AACTACCACA AGGCCTCCCG ATCTCTGGAC 
TTGGATCTTG ATCTGGTGTC CAATGAGGCT GGTGAATCGC CAGTGGCTAC TACTCCCAGC 
ACGGGATCCG TAAGTCCACC GCAGCCGGCG AGCTTGCACG ACGAGGATGC GGAGGCCACC 
ATCGCAGGTA TCCGGGCCAG GAGGCAGTTG TCCCGCGGAG ATGCCGCCAA GCTTAGCACG 
AGCGAGCTCC TGGAACGGGC GGCAGAGGCC AGAAATGCCA TCGCAGCTGA GATTCGTGCA 
CAAGCGGAGG ATGCGGCGGC CAGTGGAGGT GGAGCTCGTT GTGTGGAGAT TGTTAAGGAC 
AGCTGTGGAC TGGGTTTCTC CATCGAAGGA GGCTTTGATT CGCCGCTGGG AAATCGTCCC 
CTTATTGTCA AAAAGGTGTT CATGGGTGGT GCCGCCCAGA AGACCAACCA GGTGCGCAAT 
GGCGACGAAA TCCTTAGCAT CAATGGTGCC TCCACGTCGC GGATGACACG TGTGGATGCC 
TGGAACTATA TGAAGCAACT GCCGCTGGGA CCGGTCAAGA TTTGTTTCGC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246755.1
CDS	423..8336
Translation	MASTPKANGTTAKTATINDDYITVVEVDDPTSNPKDRLKKLVKRQSSVAEDSSFITVLTINEMELLQQRKKEEDVGSGSSPTMEEQIENVTVFRLPGERLGFGLKFQGGTRSTELVQKLYIQSCAADSPASKVSTSWGNLREGDEIVSIDGRDVRELTRIDCVRGLKDNVAIKLVVRNGHGQKPPSEEDPQQQEHLSITLNAQPPPPPPVPPRKLVRRQNSKENQAQLLIEKPLTPPPDAEYYLNLLTESIKAGSESDDTASTISTVIDKFSVGSNYSSDSSLNGHELAKVLQPFTLLEQEFLPLEQPLGHPKLLIPGNNYENVEFKTEKVNVYENVELRSPETTPTPKPRVQLATVEPKKRSIIPMPRKIPTPTKLPIEVTPPRVPILEEPKTPTNNKVDSPKTPTNELKESPISATKIPKAKFSRAKTEGEIKLHLPAPKQQSPQSKSRIPIVSTPTAQKTVPKPVSPTMNGTPLSSNIPRLLQKQKSETDLKLNLYRSKSKESSPRPPLQRANSAEAPERTFIPVLLNGNSKSSNSLESVSSIGSSSSGNSVRSPKGPKPKPPERVQSLQKTQIPKLQALPTTPPQQIPKLSMQTFKQSPPTPRSTPVGTPSPTSNREIRFKIQTYESKTQDEDKLPSLFDLVHSQENDKDKDRDSDSLKTHNSITALLAAAAAEAADLSRESPPPLVVGKCMKIVDDTNTTTCYTSSSSGEEDEDDLDASNREYICEDGEKLGPPELINGPGPSEAYFNMFWHSNMLPTIGEVEEEFSSLEPQSLTNGNLVKSEESKKLPKMEVNAGGLAQLTDDKIADHDASATRADTRDEVTTNQTQEISSSSSSSVKSQKTTTTTRVTTTKTSSSSSSSSCSSTVIPDVAFKLQPYEERDDILSEPITTIHEERRVLSEQKTLSESRTRDALTGEEQLVTSANSKSSSARFKKISSNDNLLDLGDEQDMQEQPLTATITQESSLRERLECPQDNQSIDRGTLTLSERGKQLSEKEGLTTYTECETSEKESYLEGQQVLNGKGGFVSQDENSDQPTYEREVSETLTIVNDGEKQVTKKLEQNKEIAGKKGAKLLKKTDEERRLEQEAQKLIESYQKVKKEAEKLYKLELADDDQGFDLSAFEQAEEEDKPEVSEEIKEVKDSQPVKVDIQIEEEVKTPNQVIGEVKVEDVNKEIKETVQVSQQEIKKEVKVSHQETKETFKSEEKDESNELKIVKEEVKVTHQDLREQIQEIEAVKVVHKEVKVIKESAKVTTTTSHKQATKEVIISKEDHKNDSALPAVSTTKVEIISPPLEDDLGYVLHKHIIFPQEKVKTAPTPLPKPKPKPPVPTNKPKMPVNLTKPKSAPPPVPSKRSELSGSGGKPTPTQRRGSLETAQPPKPLERIIVGVEQRDEPEKPQPIINLASVDLPNVAPNSKQATVETTPVVEMPPDELQFESHQLPDDGEKEKDLHQESNDLLSGSSSSSVTTPITTPILVSATSSMDSVQSVIEVVNGMPIISNQQTDDDDIDEVAAYDDGMHLEAEGTKDVRPVQEVTTLTLDREHESDLGTLSNHHSNISSISGINASSNIPAATLISSSIKKTSTEAATTKPAAPQIPGKMELLSSNTGTTTTTTTATHHLHQATTTKQLLVQDYLSYASPPTYSRLPPDGHEFPPNFSEPLIMHSHPLKVTTELSYEIQKGGKDESSAPPLPKTGPPATVPRKVYRQDLVINVEPAPSLTRDYQRSLSGGTPRKPSDWRKDEKSEKSVRDKIAMFSSNNELDAIPPAPATAPISSSFTRKPLNRSSENLLDSCSSSSAPSLKTRAMSVENLNDVQRQYQLAKQLPQLHVADSMYSLNTATPTPSYASLPRRSHGGSYSSGVERRISFSGEGGDAANRKAAITNILEQRRRSLSKLRGLVIPERPQLLEPILDLPEIKSQVKAASGEDSTDSGLGESHRSTVNRNCQLGAGGAANNYRSILNTNQRRPLEQQLSQPPAKPPRTSLTPLQPRSMMIPPPPPPLDQESDTDSVFSHTARVATPPEKFALTRTLSSETNTSIASSNTSTLTSGSSAGSQASCSSLGSTPAVDLTRRVLKSQVINGEAVALSSRKSILASAKCRSAKSRGQEEDNDSTDGEACSLANRRMKPISSYKLQQQQIQLGKQLVVDKLINVAAYVELTSDTDDSSRRSDTPAKISAMFIDEERKASFKGDPNQQAKVKVEQVKPMVLPMLLPSAKREPLKQQTTAELREKFERSAAAQAQTQNHSPVIHKVTQKPHHERFSSLDSLASSSSGVSSTTQNVSTTQETATEFGSFSSLGSNQSLITAQDVQQIVEEADPPLKTPEAFIIVLQRENPESSIGITLAGGSDYEAKEITIHKILSNTPAAKDGRLKKGDRILAVNGMSMRGLTHRESISVLKTPRPEVVLVVTRSESLVVKALTKKRSSLGSLSSLNEKPTELDYERKRNYHKASRSLDLDLDLVSNEAGESPVATTPSTGSVSPPQPASLHDEDAEATIAGIRARRQLSRGDAAKLSTSELLERAAEARNAIAAEIRAQAEDAAASGGGARCVEIVKDSCGLGFSIEGGFDSPLGNRPLIVKKVFMGGAAQKTNQVRNGDEILSINGASTSRMTRVDAWNYMKQLPLGPVKICFA

Target ORF information:

RefSeq Version	NM_001259826.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster big bang (bbg), transcript variant K, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259826.1

ORF Insert Sequence:

ATGGCCTCCA CACCAAAAGC GAATGGCACG ACAGCGAAGA CGGCAACCAT AAATGATGAC 
TACATAACGG TCGTGGAAGT GGATGATCCG ACGTCGAACC CGAAGGATCG CCTTAAGAAG 
CTAGTCAAGC GTCAAAGCTC TGTGGCGGAG GACTCCTCGT TCATCACAGT GCTGACGATT 
AACGAGATGG AGTTGCTTCA ACAGCGAAAA AAGGAGGAGG ATGTTGGTAG TGGCTCAAGC 
CCTACGATGG AGGAGCAAAT AGAGAATGTC ACAGTGTTCA GATTACCGGG AGAACGTCTC 
GGTTTTGGCC TAAAGTTTCA AGGAGGCACG AGGAGCACAG AGCTCGTCCA AAAACTGTAC 
ATACAATCCT GTGCTGCAGA CAGTCCTGCC TCCAAAGTGT CCACCAGCTG GGGTAATCTA 
CGAGAGGGTG ATGAGATCGT CAGTATCGAT GGTCGTGATG TCCGGGAATT GACTCGAATC 
GATTGCGTTC GCGGCCTCAA AGACAATGTG GCCATTAAGC TGGTGGTGCG AAATGGTCAC 
GGACAGAAGC CTCCCAGCGA AGAGGACCCA CAGCAGCAGG AACACCTTTC GATCACACTG 
AATGCCCAAC CACCGCCTCC GCCACCAGTG CCGCCAAGAA AGTTGGTGAG ACGGCAAAAC 
TCCAAAGAGA ACCAGGCTCA GTTGCTCATT GAGAAGCCAT TGACGCCACC ACCGGATGCG 
GAGTACTACC TGAATCTTTT AACCGAATCC ATTAAGGCGG GCTCAGAGTC GGATGATACC 
GCCAGCACAA TTTCCACTGT TATCGACAAG TTTTCCGTGG GCTCCAATTA CTCCTCGGAC 
AGTAGTCTCA ATGGCCACGA GCTGGCCAAG GTTTTGCAAC CTTTTACCCT TCTCGAGCAG 
GAGTTTCTGC CTTTGGAGCA GCCGCTAGGA CATCCCAAGT TGCTTATTCC TGGCAATAAC 
TATGAAAATG TGGAGTTCAA AACGGAGAAG GTCAATGTAT ACGAAAATGT GGAGCTCAGG 
AGTCCGGAAA CGACGCCGAC ACCAAAACCC AGAGTCCAAT TGGCCACCGT GGAGCCAAAG 
AAGCGTTCGA TCATACCCAT GCCACGAAAA ATTCCTACTC CAACCAAGCT GCCCATTGAG 
GTGACCCCAC CCCGGGTTCC CATATTGGAG GAACCCAAAA CGCCCACAAA TAACAAAGTT 
GATTCTCCCA AAACACCAAC AAACGAACTC AAGGAGTCTC CCATCAGTGC TACAAAAATA 
CCTAAGGCCA AGTTCTCGAG GGCCAAAACT GAAGGAGAGA TCAAGTTGCA TTTGCCAGCC 
CCAAAACAGC AATCACCACA AAGTAAATCT CGTATACCCA TAGTCAGTAC CCCTACAGCA 
CAAAAAACTG TTCCCAAACC AGTATCACCC ACAATGAATG GTACACCACT CAGTTCAAAT 
ATACCACGGC TGTTGCAGAA GCAGAAATCC GAAACGGATC TGAAATTGAA CTTGTATAGG 
AGTAAATCTA AGGAGTCGAG TCCCAGACCT CCCTTGCAAA GAGCGAACTC GGCAGAGGCT 
CCAGAAAGGA CCTTTATCCC TGTACTTCTT AATGGGAACT CCAAGAGCTC AAACTCCTTG 
GAGAGCGTCA GTTCGATAGG AAGTAGTAGC AGTGGAAATA GTGTAAGATC ACCAAAAGGA 
CCAAAGCCCA AGCCACCAGA GCGAGTTCAA TCGCTTCAGA AAACCCAAAT ACCCAAACTG 
CAGGCACTCC CAACTACTCC ACCTCAACAG ATACCCAAGT TGAGTATGCA GACGTTCAAG 
CAGAGCCCTC CCACACCCCG ATCAACTCCA GTGGGAACTC CATCTCCAAC AAGTAATCGA 
GAGATACGCT TCAAGATTCA GACGTACGAA AGCAAAACCC AGGACGAAGA TAAACTACCC 
AGCTTATTTG ACCTAGTGCA CAGTCAGGAG AATGATAAGG ATAAAGATAG GGATAGTGAT 
TCGCTCAAGA CCCACAATAG CATCACTGCT CTTTTGGCAG CCGCCGCTGC CGAGGCCGCT 
GATCTCTCTC GGGAGTCGCC ACCTCCGTTG GTCGTTGGCA AATGCATGAA GATCGTCGAT 
GACACCAATA CCACCACCTG CTATACCAGT TCCAGCAGCG GGGAGGAGGA CGAAGACGAT 
CTGGATGCCT CCAACCGGGA GTACATATGC GAGGACGGCG AGAAGTTGGG ACCTCCGGAG 
CTGATAAATG GCCCCGGTCC CTCGGAGGCC TACTTCAACA TGTTCTGGCA CTCGAACATG 
CTGCCCACAA TCGGCGAGGT TGAAGAGGAG TTCTCCTCCC TGGAACCCCA ATCCCTAACA 
AACGGAAACC TTGTCAAATC GGAGGAGTCG AAGAAGTTAC CCAAAATGGA AGTAAACGCC 
GGTGGACTGG CCCAGCTGAC TGATGATAAG ATTGCTGACC ATGACGCAAG TGCAACCAGA 
GCGGATACTC GCGACGAAGT CACAACAAAC CAAACCCAGG AAATCAGCTC CAGCAGTTCG 
AGTTCTGTTA AAAGTCAAAA GACCACCACA ACCACTCGAG TGACCACCAC CAAGACTAGT 
TCCAGTTCCA GTTCCTCCAG TTGCTCATCC ACTGTTATCC CAGATGTTGC CTTCAAGCTG 
CAACCCTATG AGGAAAGGGA CGATATACTC TCGGAACCCA TTACCACCAT CCACGAAGAA 
CGACGTGTGC TAAGTGAGCA GAAGACCCTC AGTGAATCGA GGACTCGCGA TGCATTAACC 
GGCGAAGAGC AGCTGGTAAC TAGTGCCAAT TCGAAGTCGA GCAGTGCCCG CTTCAAGAAG 
ATCAGCAGCA ACGACAACCT ACTCGACCTT GGCGACGAGC AGGACATGCA GGAGCAGCCG 
TTGACCGCCA CGATCACCCA GGAGTCGAGT CTGAGGGAGC GGTTGGAGTG TCCTCAGGAT 
AACCAGAGTA TCGACAGGGG TACTCTGACC CTATCCGAGC GGGGAAAGCA GCTGAGCGAG 
AAAGAAGGCC TCACCACCTA CACGGAATGT GAGACGAGTG AAAAGGAGTC CTACCTAGAG 
GGCCAACAAG TCCTCAACGG CAAAGGCGGT TTCGTTAGCC AGGATGAGAA TAGTGACCAA 
CCAACTTACG AACGAGAGGT CAGCGAGACA CTAACGATCG TCAACGATGG CGAAAAGCAG 
GTCACCAAGA AATTGGAACA GAACAAGGAA ATTGCTGGCA AAAAGGGTGC CAAGCTCCTT 
AAGAAGACCG ATGAGGAGCG ACGTCTTGAG CAGGAGGCCC AAAAGCTGAT CGAAAGCTAT 
CAGAAAGTCA AGAAAGAAGC GGAGAAGCTG TACAAGCTGG AATTGGCCGA CGATGATCAG 
GGCTTTGATC TGAGTGCCTT CGAGCAGGCG GAGGAAGAGG ATAAGCCAGA AGTTTCTGAG 
GAAATCAAAG AAGTGAAGGA TTCTCAGCCA GTAAAGGTCG ATATTCAAAT TGAAGAAGAG 
GTTAAAACAC CTAATCAAGT CATAGGGGAA GTCAAAGTGG AAGATGTGAA TAAGGAAATT 
AAAGAGACGG TACAAGTCTC TCAACAGGAA ATTAAGAAAG AGGTCAAGGT CTCACACCAA 
GAAACAAAAG AAACCTTTAA GAGTGAAGAA AAAGATGAGA GTAATGAACT CAAAATAGTT 
AAGGAGGAGG TCAAAGTAAC ACATCAGGAT CTTAGGGAAC AGATCCAGGA GATCGAGGCT 
GTCAAAGTGG TGCACAAAGA AGTAAAAGTT ATTAAGGAAA GTGCTAAAGT CACTACGACC 
ACGAGTCACA AGCAAGCAAC TAAAGAAGTA ATCATCTCGA AAGAAGATCA CAAAAATGAT 
AGCGCACTCC CTGCCGTGTC AACCACAAAA GTAGAGATCA TCTCACCTCC CCTGGAAGAC 
GACTTGGGTT ATGTCCTCCA CAAGCACATT ATTTTTCCCC AGGAAAAGGT CAAGACGGCT 
CCTACGCCAC TGCCAAAACC GAAACCCAAA CCTCCAGTGC CCACGAACAA ACCCAAAATG 
CCGGTCAATT TGACCAAACC GAAATCAGCA CCACCACCGG TGCCCAGTAA GAGGAGTGAG 
CTATCCGGAT CCGGTGGCAA GCCCACACCC ACCCAAAGAC GCGGCAGCCT GGAGACCGCC 
CAGCCTCCAA AACCACTAGA ACGTATAATT GTCGGTGTGG AGCAGCGGGA TGAGCCAGAA 
AAGCCCCAGC CAATTATCAA CTTGGCCAGC GTGGATCTGC CAAATGTGGC GCCCAACAGC 
AAGCAAGCGA CAGTGGAAAC GACTCCCGTG GTCGAAATGC CACCGGATGA GCTCCAATTC 
GAGAGTCACC AACTGCCGGA TGACGGTGAA AAGGAAAAGG ACTTGCACCA GGAGTCAAAT 
GACCTGCTCA GCGGGTCGTC ATCGTCGTCG GTAACCACGC CCATAACAAC GCCAATTTTG 
GTATCTGCCA CCTCGTCGAT GGACTCGGTG CAAAGTGTCA TCGAGGTCGT CAATGGCATG 
CCCATCATCA GCAATCAGCA AACTGACGAC GACGACATTG ATGAAGTTGC AGCGTATGAC 
GATGGGATGC ATTTGGAGGC CGAAGGTACG AAGGATGTTC GTCCCGTCCA GGAGGTGACA 
ACGTTGACGC TCGACAGGGA ACACGAATCA GATCTTGGTA CCCTCAGTAA CCACCACAGC 
AATATAAGCA GCATTAGCGG CATTAACGCC AGTAGCAACA TCCCGGCAGC AACTTTAATC 
AGCAGCAGCA TTAAGAAGAC ATCCACTGAG GCGGCAACCA CAAAACCTGC TGCACCCCAG 
ATCCCCGGCA AAATGGAGCT GCTGAGCAGC AACACAGGAA CAACAACGAC AACGACGACA 
GCAACGCACC ACCTGCATCA GGCAACCACC ACTAAACAGC TGTTGGTGCA GGATTACCTC 
AGCTACGCCT CACCACCCAC ATATTCCCGC CTGCCGCCCG ATGGTCATGA ATTTCCACCC 
AATTTCAGCG AACCACTGAT CATGCACAGC CACCCACTAA AGGTCACCAC CGAGTTGAGT 
TACGAGATAC AGAAAGGCGG AAAGGATGAG TCCTCTGCAC CACCGCTTCC CAAAACGGGG 
CCACCAGCCA CAGTTCCCAG GAAAGTATAT CGCCAGGATT TGGTCATCAA CGTAGAGCCA 
GCACCCAGTT TAACCAGGGA CTATCAGAGA TCTCTGAGTG GTGGTACTCC CCGGAAGCCT 
AGCGATTGGC GCAAGGATGA AAAGTCGGAG AAATCAGTGC GCGACAAGAT TGCCATGTTC 
TCGTCAAACA ACGAACTGGA TGCCATACCG CCAGCTCCGG CAACAGCCCC CATTTCAAGC 
TCCTTCACCC GGAAGCCCTT GAATAGGAGC AGTGAAAATC TTCTGGACAG TTGCTCATCA 
TCCTCGGCTC CTTCGCTGAA AACTCGCGCG ATGAGCGTGG AAAATTTGAA TGATGTTCAG 
CGGCAATATC AGTTGGCCAA ACAGCTGCCC CAGTTGCACG TGGCCGACTC TATGTATTCC 
CTGAACACAG CCACGCCCAC ACCGAGCTAC GCCTCTTTGC CGAGAAGAAG CCACGGGGGA 
TCTTATTCTT CTGGAGTGGA GAGAAGGATC AGTTTCTCGG GAGAAGGCGG AGATGCGGCC 
AACCGAAAGG CAGCCATTAC AAATATCCTA GAGCAAAGAA GAAGAAGTTT GTCGAAACTG 
CGAGGATTGG TCATACCAGA GAGACCCCAA CTGCTGGAAC CCATCCTGGA TCTGCCGGAG 
ATCAAAAGCC AGGTAAAAGC AGCCAGCGGA GAGGATAGTA CGGATAGCGG ATTAGGCGAA 
AGCCACCGCA GTACGGTGAA TAGGAATTGC CAATTGGGTG CAGGAGGTGC CGCAAATAAC 
TATCGCAGCA TTTTAAATAC CAATCAAAGA AGACCCCTGG AGCAACAGCT CTCTCAACCA 
CCAGCCAAAC CACCCAGGAC ATCGTTGACA CCACTTCAGC CGAGAAGCAT GATGATACCA 
CCACCACCAC CTCCTTTAGA TCAGGAAAGT GATACGGATT CGGTGTTTTC GCACACTGCT 
AGGGTTGCCA CTCCGCCGGA GAAGTTTGCA CTAACCAGAA CCCTCAGCTC CGAAACAAAC 
ACTTCGATAG CTAGTTCAAA CACCTCTACT CTAACATCAG GATCTTCTGC CGGATCTCAA 
GCCAGTTGCA GTTCGCTGGG CAGCACACCA GCGGTGGATC TAACCAGGCG GGTTCTCAAG 
AGCCAGGTGA TCAATGGCGA AGCCGTTGCG CTCTCCAGTC GCAAAAGTAT TCTGGCCTCG 
GCCAAATGTC GCAGTGCCAA GAGTCGAGGT CAAGAGGAGG ATAACGACAG CACCGATGGT 
GAAGCCTGTT CCCTGGCCAA TCGTAGAATG AAGCCGATTT CCAGCTATAA GCTCCAGCAG 
CAACAGATCC AACTGGGCAA ACAACTGGTA GTGGATAAGC TGATCAATGT GGCCGCTTAT 
GTGGAACTAA CCTCGGATAC GGACGACAGC AGCCGTAGAT CTGATACGCC TGCCAAGATC 
AGTGCCATGT TCATAGACGA GGAGCGCAAG GCCAGCTTCA AAGGAGATCC TAACCAGCAG 
GCTAAGGTCA AAGTGGAGCA GGTCAAACCT ATGGTCCTCC CAATGCTGCT ACCATCTGCG 
AAAAGGGAAC CGCTGAAGCA ACAGACCACC GCCGAACTGC GCGAAAAGTT CGAAAGGAGT 
GCTGCAGCCC AAGCTCAAAC CCAAAACCAC TCGCCCGTAA TCCATAAGGT CACCCAGAAG 
CCGCATCATG AGCGATTCTC CTCGCTGGAC TCCCTGGCGT CCAGTTCCTC CGGCGTTAGT 
TCCACCACCC AAAACGTGAG CACCACCCAG GAAACGGCCA CCGAATTCGG CAGCTTTTCC 
TCGCTGGGTA GCAACCAGAG TTTGATCACC GCCCAGGATG TCCAGCAGAT AGTCGAGGAG 
GCTGATCCTC CACTAAAGAC CCCAGAAGCG TTCATCATCG TCCTGCAGAG GGAAAATCCC 
GAGAGCAGCA TCGGTATCAC TTTGGCCGGC GGTTCTGATT ATGAGGCTAA GGAAATTACG 
ATCCATAAGA TCCTGAGCAA CACGCCAGCT GCCAAGGATG GACGTCTAAA GAAGGGTGAT 
CGAATCCTTG CCGTCAATGG AATGAGCATG CGCGGACTGA CGCACCGCGA ATCCATCAGT 
GTGCTTAAGA CCCCCCGACC TGAGGTCGTG CTAGTGGTGA CCCGCTCCGA GTCGCTGGTG 
GTGAAGGCGC TGACCAAGAA ACGATCCTCC CTGGGATCAC TTAGCTCGCT CAATGAGAAA 
CCCACGGAGC TGGACTATGA ACGCAAGAGG AACTACCACA AGGCCTCCCG ATCTCTGGAC 
TTGGATCTTG ATCTGGTGTC CAATGAGGCT GGTGAATCGC CAGTGGCTAC TACTCCCAGC 
ACGGGATCCG TAAGTCCACC GCAGCCGGCG AGCTTGCACG ACGAGGATGC GGAGGCCACC 
ATCGCAGGTA TCCGGGCCAG GAGGCAGTTG TCCCGCGGAG ATGCCGCCAA GCTTAGCACG 
AGCGAGCTCC TGGAACGGGC GGCAGAGGCC AGAAATGCCA TCGCAGCTGA GATTCGTGCA 
CAAGCGGAGG ATGCGGCGGC CAGTGGAGGT GGAGCTCGTT GTGTGGAGAT TGTTAAGGAC 
AGCTGTGGAC TGGGTTTCTC CATCGAAGGA GGCTTTGATT CGCCGCTGGG AAATCGTCCC 
CTTATTGTCA AAAAGGTGTT CATGGGTGGT GCCGCCCAGA AGACCAACCA GGTGCGCAAT 
GGCGACGAAA TCCTTAGCAT CAATGGTGCC TCCACGTCGC GGATGACACG TGTGGATGCC 
TGGAACTATA TGAAGCAACT GCCGCTGGGA CCGGTCAAGA TTTGTTTCGC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bbg ( NM_140448.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648705.2 Drosophila melanogaster big bang (bbg), transcript variant C, mRNA.

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CloneID	OFa18500
Clone ID Related Accession (Same CDS sequence)	NM_001259826.1 , NM_140448.3
Accession Version	NM_140448.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	big bang, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On May 8, 2012 this sequence version replaced NM_140448.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCTCCA CACCAAAAGC GAATGGCACG ACAGCGAAGA CGGCAACCAT AAATGATGAC 
TACATAACGG TCGTGGAAGT GGATGATCCG ACGTCGAACC CGAAGGATCG CCTTAAGAAG 
CTAGTCAAGC GTCAAAGCTC TGTGGCGGAG GACTCCTCGT TCATCACAGT GCTGACGATT 
AACGAGATGG AGTTGCTTCA ACAGCGAAAA AAGGAGGAGG ATGTTGGTAG TGGCTCAAGC 
CCTACGATGG AGGAGCAAAT AGAGAATGTC ACAGTGTTCA GATTACCGGG AGAACGTCTC 
GGTTTTGGCC TAAAGTTTCA AGGAGGCACG AGGAGCACAG AGCTCGTCCA AAAACTGTAC 
ATACAATCCT GTGCTGCAGA CAGTCCTGCC TCCAAAGTGT CCACCAGCTG GGGTAATCTA 
CGAGAGGGTG ATGAGATCGT CAGTATCGAT GGTCGTGATG TCCGGGAATT GACTCGAATC 
GATTGCGTTC GCGGCCTCAA AGACAATGTG GCCATTAAGC TGGTGGTGCG AAATGGTCAC 
GGACAGAAGC CTCCCAGCGA AGAGGACCCA CAGCAGCAGG AACACCTTTC GATCACACTG 
AATGCCCAAC CACCGCCTCC GCCACCAGTG CCGCCAAGAA AGTTGGTGAG ACGGCAAAAC 
TCCAAAGAGA ACCAGGCTCA GTTGCTCATT GAGAAGCCAT TGACGCCACC ACCGGATGCG 
GAGTACTACC TGAATCTTTT AACCGAATCC ATTAAGGCGG GCTCAGAGTC GGATGATACC 
GCCAGCACAA TTTCCACTGT TATCGACAAG TTTTCCGTGG GCTCCAATTA CTCCTCGGAC 
AGTAGTCTCA ATGGCCACGA GCTGGCCAAG GTTTTGCAAC CTTTTACCCT TCTCGAGCAG 
GAGTTTCTGC CTTTGGAGCA GCCGCTAGGA CATCCCAAGT TGCTTATTCC TGGCAATAAC 
TATGAAAATG TGGAGTTCAA AACGGAGAAG GTCAATGTAT ACGAAAATGT GGAGCTCAGG 
AGTCCGGAAA CGACGCCGAC ACCAAAACCC AGAGTCCAAT TGGCCACCGT GGAGCCAAAG 
AAGCGTTCGA TCATACCCAT GCCACGAAAA ATTCCTACTC CAACCAAGCT GCCCATTGAG 
GTGACCCCAC CCCGGGTTCC CATATTGGAG GAACCCAAAA CGCCCACAAA TAACAAAGTT 
GATTCTCCCA AAACACCAAC AAACGAACTC AAGGAGTCTC CCATCAGTGC TACAAAAATA 
CCTAAGGCCA AGTTCTCGAG GGCCAAAACT GAAGGAGAGA TCAAGTTGCA TTTGCCAGCC 
CCAAAACAGC AATCACCACA AAGTAAATCT CGTATACCCA TAGTCAGTAC CCCTACAGCA 
CAAAAAACTG TTCCCAAACC AGTATCACCC ACAATGAATG GTACACCACT CAGTTCAAAT 
ATACCACGGC TGTTGCAGAA GCAGAAATCC GAAACGGATC TGAAATTGAA CTTGTATAGG 
AGTAAATCTA AGGAGTCGAG TCCCAGACCT CCCTTGCAAA GAGCGAACTC GGCAGAGGCT 
CCAGAAAGGA CCTTTATCCC TGTACTTCTT AATGGGAACT CCAAGAGCTC AAACTCCTTG 
GAGAGCGTCA GTTCGATAGG AAGTAGTAGC AGTGGAAATA GTGTAAGATC ACCAAAAGGA 
CCAAAGCCCA AGCCACCAGA GCGAGTTCAA TCGCTTCAGA AAACCCAAAT ACCCAAACTG 
CAGGCACTCC CAACTACTCC ACCTCAACAG ATACCCAAGT TGAGTATGCA GACGTTCAAG 
CAGAGCCCTC CCACACCCCG ATCAACTCCA GTGGGAACTC CATCTCCAAC AAGTAATCGA 
GAGATACGCT TCAAGATTCA GACGTACGAA AGCAAAACCC AGGACGAAGA TAAACTACCC 
AGCTTATTTG ACCTAGTGCA CAGTCAGGAG AATGATAAGG ATAAAGATAG GGATAGTGAT 
TCGCTCAAGA CCCACAATAG CATCACTGCT CTTTTGGCAG CCGCCGCTGC CGAGGCCGCT 
GATCTCTCTC GGGAGTCGCC ACCTCCGTTG GTCGTTGGCA AATGCATGAA GATCGTCGAT 
GACACCAATA CCACCACCTG CTATACCAGT TCCAGCAGCG GGGAGGAGGA CGAAGACGAT 
CTGGATGCCT CCAACCGGGA GTACATATGC GAGGACGGCG AGAAGTTGGG ACCTCCGGAG 
CTGATAAATG GCCCCGGTCC CTCGGAGGCC TACTTCAACA TGTTCTGGCA CTCGAACATG 
CTGCCCACAA TCGGCGAGGT TGAAGAGGAG TTCTCCTCCC TGGAACCCCA ATCCCTAACA 
AACGGAAACC TTGTCAAATC GGAGGAGTCG AAGAAGTTAC CCAAAATGGA AGTAAACGCC 
GGTGGACTGG CCCAGCTGAC TGATGATAAG ATTGCTGACC ATGACGCAAG TGCAACCAGA 
GCGGATACTC GCGACGAAGT CACAACAAAC CAAACCCAGG AAATCAGCTC CAGCAGTTCG 
AGTTCTGTTA AAAGTCAAAA GACCACCACA ACCACTCGAG TGACCACCAC CAAGACTAGT 
TCCAGTTCCA GTTCCTCCAG TTGCTCATCC ACTGTTATCC CAGATGTTGC CTTCAAGCTG 
CAACCCTATG AGGAAAGGGA CGATATACTC TCGGAACCCA TTACCACCAT CCACGAAGAA 
CGACGTGTGC TAAGTGAGCA GAAGACCCTC AGTGAATCGA GGACTCGCGA TGCATTAACC 
GGCGAAGAGC AGCTGGTAAC TAGTGCCAAT TCGAAGTCGA GCAGTGCCCG CTTCAAGAAG 
ATCAGCAGCA ACGACAACCT ACTCGACCTT GGCGACGAGC AGGACATGCA GGAGCAGCCG 
TTGACCGCCA CGATCACCCA GGAGTCGAGT CTGAGGGAGC GGTTGGAGTG TCCTCAGGAT 
AACCAGAGTA TCGACAGGGG TACTCTGACC CTATCCGAGC GGGGAAAGCA GCTGAGCGAG 
AAAGAAGGCC TCACCACCTA CACGGAATGT GAGACGAGTG AAAAGGAGTC CTACCTAGAG 
GGCCAACAAG TCCTCAACGG CAAAGGCGGT TTCGTTAGCC AGGATGAGAA TAGTGACCAA 
CCAACTTACG AACGAGAGGT CAGCGAGACA CTAACGATCG TCAACGATGG CGAAAAGCAG 
GTCACCAAGA AATTGGAACA GAACAAGGAA ATTGCTGGCA AAAAGGGTGC CAAGCTCCTT 
AAGAAGACCG ATGAGGAGCG ACGTCTTGAG CAGGAGGCCC AAAAGCTGAT CGAAAGCTAT 
CAGAAAGTCA AGAAAGAAGC GGAGAAGCTG TACAAGCTGG AATTGGCCGA CGATGATCAG 
GGCTTTGATC TGAGTGCCTT CGAGCAGGCG GAGGAAGAGG ATAAGCCAGA AGTTTCTGAG 
GAAATCAAAG AAGTGAAGGA TTCTCAGCCA GTAAAGGTCG ATATTCAAAT TGAAGAAGAG 
GTTAAAACAC CTAATCAAGT CATAGGGGAA GTCAAAGTGG AAGATGTGAA TAAGGAAATT 
AAAGAGACGG TACAAGTCTC TCAACAGGAA ATTAAGAAAG AGGTCAAGGT CTCACACCAA 
GAAACAAAAG AAACCTTTAA GAGTGAAGAA AAAGATGAGA GTAATGAACT CAAAATAGTT 
AAGGAGGAGG TCAAAGTAAC ACATCAGGAT CTTAGGGAAC AGATCCAGGA GATCGAGGCT 
GTCAAAGTGG TGCACAAAGA AGTAAAAGTT ATTAAGGAAA GTGCTAAAGT CACTACGACC 
ACGAGTCACA AGCAAGCAAC TAAAGAAGTA ATCATCTCGA AAGAAGATCA CAAAAATGAT 
AGCGCACTCC CTGCCGTGTC AACCACAAAA GTAGAGATCA TCTCACCTCC CCTGGAAGAC 
GACTTGGGTT ATGTCCTCCA CAAGCACATT ATTTTTCCCC AGGAAAAGGT CAAGACGGCT 
CCTACGCCAC TGCCAAAACC GAAACCCAAA CCTCCAGTGC CCACGAACAA ACCCAAAATG 
CCGGTCAATT TGACCAAACC GAAATCAGCA CCACCACCGG TGCCCAGTAA GAGGAGTGAG 
CTATCCGGAT CCGGTGGCAA GCCCACACCC ACCCAAAGAC GCGGCAGCCT GGAGACCGCC 
CAGCCTCCAA AACCACTAGA ACGTATAATT GTCGGTGTGG AGCAGCGGGA TGAGCCAGAA 
AAGCCCCAGC CAATTATCAA CTTGGCCAGC GTGGATCTGC CAAATGTGGC GCCCAACAGC 
AAGCAAGCGA CAGTGGAAAC GACTCCCGTG GTCGAAATGC CACCGGATGA GCTCCAATTC 
GAGAGTCACC AACTGCCGGA TGACGGTGAA AAGGAAAAGG ACTTGCACCA GGAGTCAAAT 
GACCTGCTCA GCGGGTCGTC ATCGTCGTCG GTAACCACGC CCATAACAAC GCCAATTTTG 
GTATCTGCCA CCTCGTCGAT GGACTCGGTG CAAAGTGTCA TCGAGGTCGT CAATGGCATG 
CCCATCATCA GCAATCAGCA AACTGACGAC GACGACATTG ATGAAGTTGC AGCGTATGAC 
GATGGGATGC ATTTGGAGGC CGAAGGTACG AAGGATGTTC GTCCCGTCCA GGAGGTGACA 
ACGTTGACGC TCGACAGGGA ACACGAATCA GATCTTGGTA CCCTCAGTAA CCACCACAGC 
AATATAAGCA GCATTAGCGG CATTAACGCC AGTAGCAACA TCCCGGCAGC AACTTTAATC 
AGCAGCAGCA TTAAGAAGAC ATCCACTGAG GCGGCAACCA CAAAACCTGC TGCACCCCAG 
ATCCCCGGCA AAATGGAGCT GCTGAGCAGC AACACAGGAA CAACAACGAC AACGACGACA 
GCAACGCACC ACCTGCATCA GGCAACCACC ACTAAACAGC TGTTGGTGCA GGATTACCTC 
AGCTACGCCT CACCACCCAC ATATTCCCGC CTGCCGCCCG ATGGTCATGA ATTTCCACCC 
AATTTCAGCG AACCACTGAT CATGCACAGC CACCCACTAA AGGTCACCAC CGAGTTGAGT 
TACGAGATAC AGAAAGGCGG AAAGGATGAG TCCTCTGCAC CACCGCTTCC CAAAACGGGG 
CCACCAGCCA CAGTTCCCAG GAAAGTATAT CGCCAGGATT TGGTCATCAA CGTAGAGCCA 
GCACCCAGTT TAACCAGGGA CTATCAGAGA TCTCTGAGTG GTGGTACTCC CCGGAAGCCT 
AGCGATTGGC GCAAGGATGA AAAGTCGGAG AAATCAGTGC GCGACAAGAT TGCCATGTTC 
TCGTCAAACA ACGAACTGGA TGCCATACCG CCAGCTCCGG CAACAGCCCC CATTTCAAGC 
TCCTTCACCC GGAAGCCCTT GAATAGGAGC AGTGAAAATC TTCTGGACAG TTGCTCATCA 
TCCTCGGCTC CTTCGCTGAA AACTCGCGCG ATGAGCGTGG AAAATTTGAA TGATGTTCAG 
CGGCAATATC AGTTGGCCAA ACAGCTGCCC CAGTTGCACG TGGCCGACTC TATGTATTCC 
CTGAACACAG CCACGCCCAC ACCGAGCTAC GCCTCTTTGC CGAGAAGAAG CCACGGGGGA 
TCTTATTCTT CTGGAGTGGA GAGAAGGATC AGTTTCTCGG GAGAAGGCGG AGATGCGGCC 
AACCGAAAGG CAGCCATTAC AAATATCCTA GAGCAAAGAA GAAGAAGTTT GTCGAAACTG 
CGAGGATTGG TCATACCAGA GAGACCCCAA CTGCTGGAAC CCATCCTGGA TCTGCCGGAG 
ATCAAAAGCC AGGTAAAAGC AGCCAGCGGA GAGGATAGTA CGGATAGCGG ATTAGGCGAA 
AGCCACCGCA GTACGGTGAA TAGGAATTGC CAATTGGGTG CAGGAGGTGC CGCAAATAAC 
TATCGCAGCA TTTTAAATAC CAATCAAAGA AGACCCCTGG AGCAACAGCT CTCTCAACCA 
CCAGCCAAAC CACCCAGGAC ATCGTTGACA CCACTTCAGC CGAGAAGCAT GATGATACCA 
CCACCACCAC CTCCTTTAGA TCAGGAAAGT GATACGGATT CGGTGTTTTC GCACACTGCT 
AGGGTTGCCA CTCCGCCGGA GAAGTTTGCA CTAACCAGAA CCCTCAGCTC CGAAACAAAC 
ACTTCGATAG CTAGTTCAAA CACCTCTACT CTAACATCAG GATCTTCTGC CGGATCTCAA 
GCCAGTTGCA GTTCGCTGGG CAGCACACCA GCGGTGGATC TAACCAGGCG GGTTCTCAAG 
AGCCAGGTGA TCAATGGCGA AGCCGTTGCG CTCTCCAGTC GCAAAAGTAT TCTGGCCTCG 
GCCAAATGTC GCAGTGCCAA GAGTCGAGGT CAAGAGGAGG ATAACGACAG CACCGATGGT 
GAAGCCTGTT CCCTGGCCAA TCGTAGAATG AAGCCGATTT CCAGCTATAA GCTCCAGCAG 
CAACAGATCC AACTGGGCAA ACAACTGGTA GTGGATAAGC TGATCAATGT GGCCGCTTAT 
GTGGAACTAA CCTCGGATAC GGACGACAGC AGCCGTAGAT CTGATACGCC TGCCAAGATC 
AGTGCCATGT TCATAGACGA GGAGCGCAAG GCCAGCTTCA AAGGAGATCC TAACCAGCAG 
GCTAAGGTCA AAGTGGAGCA GGTCAAACCT ATGGTCCTCC CAATGCTGCT ACCATCTGCG 
AAAAGGGAAC CGCTGAAGCA ACAGACCACC GCCGAACTGC GCGAAAAGTT CGAAAGGAGT 
GCTGCAGCCC AAGCTCAAAC CCAAAACCAC TCGCCCGTAA TCCATAAGGT CACCCAGAAG 
CCGCATCATG AGCGATTCTC CTCGCTGGAC TCCCTGGCGT CCAGTTCCTC CGGCGTTAGT 
TCCACCACCC AAAACGTGAG CACCACCCAG GAAACGGCCA CCGAATTCGG CAGCTTTTCC 
TCGCTGGGTA GCAACCAGAG TTTGATCACC GCCCAGGATG TCCAGCAGAT AGTCGAGGAG 
GCTGATCCTC CACTAAAGAC CCCAGAAGCG TTCATCATCG TCCTGCAGAG GGAAAATCCC 
GAGAGCAGCA TCGGTATCAC TTTGGCCGGC GGTTCTGATT ATGAGGCTAA GGAAATTACG 
ATCCATAAGA TCCTGAGCAA CACGCCAGCT GCCAAGGATG GACGTCTAAA GAAGGGTGAT 
CGAATCCTTG CCGTCAATGG AATGAGCATG CGCGGACTGA CGCACCGCGA ATCCATCAGT 
GTGCTTAAGA CCCCCCGACC TGAGGTCGTG CTAGTGGTGA CCCGCTCCGA GTCGCTGGTG 
GTGAAGGCGC TGACCAAGAA ACGATCCTCC CTGGGATCAC TTAGCTCGCT CAATGAGAAA 
CCCACGGAGC TGGACTATGA ACGCAAGAGG AACTACCACA AGGCCTCCCG ATCTCTGGAC 
TTGGATCTTG ATCTGGTGTC CAATGAGGCT GGTGAATCGC CAGTGGCTAC TACTCCCAGC 
ACGGGATCCG TAAGTCCACC GCAGCCGGCG AGCTTGCACG ACGAGGATGC GGAGGCCACC 
ATCGCAGGTA TCCGGGCCAG GAGGCAGTTG TCCCGCGGAG ATGCCGCCAA GCTTAGCACG 
AGCGAGCTCC TGGAACGGGC GGCAGAGGCC AGAAATGCCA TCGCAGCTGA GATTCGTGCA 
CAAGCGGAGG ATGCGGCGGC CAGTGGAGGT GGAGCTCGTT GTGTGGAGAT TGTTAAGGAC 
AGCTGTGGAC TGGGTTTCTC CATCGAAGGA GGCTTTGATT CGCCGCTGGG AAATCGTCCC 
CTTATTGTCA AAAAGGTGTT CATGGGTGGT GCCGCCCAGA AGACCAACCA GGTGCGCAAT 
GGCGACGAAA TCCTTAGCAT CAATGGTGCC TCCACGTCGC GGATGACACG TGTGGATGCC 
TGGAACTATA TGAAGCAACT GCCGCTGGGA CCGGTCAAGA TTTGTTTCGC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648705.2
CDS	407..8320
Translation	MASTPKANGTTAKTATINDDYITVVEVDDPTSNPKDRLKKLVKRQSSVAEDSSFITVLTINEMELLQQRKKEEDVGSGSSPTMEEQIENVTVFRLPGERLGFGLKFQGGTRSTELVQKLYIQSCAADSPASKVSTSWGNLREGDEIVSIDGRDVRELTRIDCVRGLKDNVAIKLVVRNGHGQKPPSEEDPQQQEHLSITLNAQPPPPPPVPPRKLVRRQNSKENQAQLLIEKPLTPPPDAEYYLNLLTESIKAGSESDDTASTISTVIDKFSVGSNYSSDSSLNGHELAKVLQPFTLLEQEFLPLEQPLGHPKLLIPGNNYENVEFKTEKVNVYENVELRSPETTPTPKPRVQLATVEPKKRSIIPMPRKIPTPTKLPIEVTPPRVPILEEPKTPTNNKVDSPKTPTNELKESPISATKIPKAKFSRAKTEGEIKLHLPAPKQQSPQSKSRIPIVSTPTAQKTVPKPVSPTMNGTPLSSNIPRLLQKQKSETDLKLNLYRSKSKESSPRPPLQRANSAEAPERTFIPVLLNGNSKSSNSLESVSSIGSSSSGNSVRSPKGPKPKPPERVQSLQKTQIPKLQALPTTPPQQIPKLSMQTFKQSPPTPRSTPVGTPSPTSNREIRFKIQTYESKTQDEDKLPSLFDLVHSQENDKDKDRDSDSLKTHNSITALLAAAAAEAADLSRESPPPLVVGKCMKIVDDTNTTTCYTSSSSGEEDEDDLDASNREYICEDGEKLGPPELINGPGPSEAYFNMFWHSNMLPTIGEVEEEFSSLEPQSLTNGNLVKSEESKKLPKMEVNAGGLAQLTDDKIADHDASATRADTRDEVTTNQTQEISSSSSSSVKSQKTTTTTRVTTTKTSSSSSSSSCSSTVIPDVAFKLQPYEERDDILSEPITTIHEERRVLSEQKTLSESRTRDALTGEEQLVTSANSKSSSARFKKISSNDNLLDLGDEQDMQEQPLTATITQESSLRERLECPQDNQSIDRGTLTLSERGKQLSEKEGLTTYTECETSEKESYLEGQQVLNGKGGFVSQDENSDQPTYEREVSETLTIVNDGEKQVTKKLEQNKEIAGKKGAKLLKKTDEERRLEQEAQKLIESYQKVKKEAEKLYKLELADDDQGFDLSAFEQAEEEDKPEVSEEIKEVKDSQPVKVDIQIEEEVKTPNQVIGEVKVEDVNKEIKETVQVSQQEIKKEVKVSHQETKETFKSEEKDESNELKIVKEEVKVTHQDLREQIQEIEAVKVVHKEVKVIKESAKVTTTTSHKQATKEVIISKEDHKNDSALPAVSTTKVEIISPPLEDDLGYVLHKHIIFPQEKVKTAPTPLPKPKPKPPVPTNKPKMPVNLTKPKSAPPPVPSKRSELSGSGGKPTPTQRRGSLETAQPPKPLERIIVGVEQRDEPEKPQPIINLASVDLPNVAPNSKQATVETTPVVEMPPDELQFESHQLPDDGEKEKDLHQESNDLLSGSSSSSVTTPITTPILVSATSSMDSVQSVIEVVNGMPIISNQQTDDDDIDEVAAYDDGMHLEAEGTKDVRPVQEVTTLTLDREHESDLGTLSNHHSNISSISGINASSNIPAATLISSSIKKTSTEAATTKPAAPQIPGKMELLSSNTGTTTTTTTATHHLHQATTTKQLLVQDYLSYASPPTYSRLPPDGHEFPPNFSEPLIMHSHPLKVTTELSYEIQKGGKDESSAPPLPKTGPPATVPRKVYRQDLVINVEPAPSLTRDYQRSLSGGTPRKPSDWRKDEKSEKSVRDKIAMFSSNNELDAIPPAPATAPISSSFTRKPLNRSSENLLDSCSSSSAPSLKTRAMSVENLNDVQRQYQLAKQLPQLHVADSMYSLNTATPTPSYASLPRRSHGGSYSSGVERRISFSGEGGDAANRKAAITNILEQRRRSLSKLRGLVIPERPQLLEPILDLPEIKSQVKAASGEDSTDSGLGESHRSTVNRNCQLGAGGAANNYRSILNTNQRRPLEQQLSQPPAKPPRTSLTPLQPRSMMIPPPPPPLDQESDTDSVFSHTARVATPPEKFALTRTLSSETNTSIASSNTSTLTSGSSAGSQASCSSLGSTPAVDLTRRVLKSQVINGEAVALSSRKSILASAKCRSAKSRGQEEDNDSTDGEACSLANRRMKPISSYKLQQQQIQLGKQLVVDKLINVAAYVELTSDTDDSSRRSDTPAKISAMFIDEERKASFKGDPNQQAKVKVEQVKPMVLPMLLPSAKREPLKQQTTAELREKFERSAAAQAQTQNHSPVIHKVTQKPHHERFSSLDSLASSSSGVSSTTQNVSTTQETATEFGSFSSLGSNQSLITAQDVQQIVEEADPPLKTPEAFIIVLQRENPESSIGITLAGGSDYEAKEITIHKILSNTPAAKDGRLKKGDRILAVNGMSMRGLTHRESISVLKTPRPEVVLVVTRSESLVVKALTKKRSSLGSLSSLNEKPTELDYERKRNYHKASRSLDLDLDLVSNEAGESPVATTPSTGSVSPPQPASLHDEDAEATIAGIRARRQLSRGDAAKLSTSELLERAAEARNAIAAEIRAQAEDAAASGGGARCVEIVKDSCGLGFSIEGGFDSPLGNRPLIVKKVFMGGAAQKTNQVRNGDEILSINGASTSRMTRVDAWNYMKQLPLGPVKICFA

Target ORF information:

RefSeq Version	NM_140448.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster big bang (bbg), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140448.3

ORF Insert Sequence:

ATGGCCTCCA CACCAAAAGC GAATGGCACG ACAGCGAAGA CGGCAACCAT AAATGATGAC 
TACATAACGG TCGTGGAAGT GGATGATCCG ACGTCGAACC CGAAGGATCG CCTTAAGAAG 
CTAGTCAAGC GTCAAAGCTC TGTGGCGGAG GACTCCTCGT TCATCACAGT GCTGACGATT 
AACGAGATGG AGTTGCTTCA ACAGCGAAAA AAGGAGGAGG ATGTTGGTAG TGGCTCAAGC 
CCTACGATGG AGGAGCAAAT AGAGAATGTC ACAGTGTTCA GATTACCGGG AGAACGTCTC 
GGTTTTGGCC TAAAGTTTCA AGGAGGCACG AGGAGCACAG AGCTCGTCCA AAAACTGTAC 
ATACAATCCT GTGCTGCAGA CAGTCCTGCC TCCAAAGTGT CCACCAGCTG GGGTAATCTA 
CGAGAGGGTG ATGAGATCGT CAGTATCGAT GGTCGTGATG TCCGGGAATT GACTCGAATC 
GATTGCGTTC GCGGCCTCAA AGACAATGTG GCCATTAAGC TGGTGGTGCG AAATGGTCAC 
GGACAGAAGC CTCCCAGCGA AGAGGACCCA CAGCAGCAGG AACACCTTTC GATCACACTG 
AATGCCCAAC CACCGCCTCC GCCACCAGTG CCGCCAAGAA AGTTGGTGAG ACGGCAAAAC 
TCCAAAGAGA ACCAGGCTCA GTTGCTCATT GAGAAGCCAT TGACGCCACC ACCGGATGCG 
GAGTACTACC TGAATCTTTT AACCGAATCC ATTAAGGCGG GCTCAGAGTC GGATGATACC 
GCCAGCACAA TTTCCACTGT TATCGACAAG TTTTCCGTGG GCTCCAATTA CTCCTCGGAC 
AGTAGTCTCA ATGGCCACGA GCTGGCCAAG GTTTTGCAAC CTTTTACCCT TCTCGAGCAG 
GAGTTTCTGC CTTTGGAGCA GCCGCTAGGA CATCCCAAGT TGCTTATTCC TGGCAATAAC 
TATGAAAATG TGGAGTTCAA AACGGAGAAG GTCAATGTAT ACGAAAATGT GGAGCTCAGG 
AGTCCGGAAA CGACGCCGAC ACCAAAACCC AGAGTCCAAT TGGCCACCGT GGAGCCAAAG 
AAGCGTTCGA TCATACCCAT GCCACGAAAA ATTCCTACTC CAACCAAGCT GCCCATTGAG 
GTGACCCCAC CCCGGGTTCC CATATTGGAG GAACCCAAAA CGCCCACAAA TAACAAAGTT 
GATTCTCCCA AAACACCAAC AAACGAACTC AAGGAGTCTC CCATCAGTGC TACAAAAATA 
CCTAAGGCCA AGTTCTCGAG GGCCAAAACT GAAGGAGAGA TCAAGTTGCA TTTGCCAGCC 
CCAAAACAGC AATCACCACA AAGTAAATCT CGTATACCCA TAGTCAGTAC CCCTACAGCA 
CAAAAAACTG TTCCCAAACC AGTATCACCC ACAATGAATG GTACACCACT CAGTTCAAAT 
ATACCACGGC TGTTGCAGAA GCAGAAATCC GAAACGGATC TGAAATTGAA CTTGTATAGG 
AGTAAATCTA AGGAGTCGAG TCCCAGACCT CCCTTGCAAA GAGCGAACTC GGCAGAGGCT 
CCAGAAAGGA CCTTTATCCC TGTACTTCTT AATGGGAACT CCAAGAGCTC AAACTCCTTG 
GAGAGCGTCA GTTCGATAGG AAGTAGTAGC AGTGGAAATA GTGTAAGATC ACCAAAAGGA 
CCAAAGCCCA AGCCACCAGA GCGAGTTCAA TCGCTTCAGA AAACCCAAAT ACCCAAACTG 
CAGGCACTCC CAACTACTCC ACCTCAACAG ATACCCAAGT TGAGTATGCA GACGTTCAAG 
CAGAGCCCTC CCACACCCCG ATCAACTCCA GTGGGAACTC CATCTCCAAC AAGTAATCGA 
GAGATACGCT TCAAGATTCA GACGTACGAA AGCAAAACCC AGGACGAAGA TAAACTACCC 
AGCTTATTTG ACCTAGTGCA CAGTCAGGAG AATGATAAGG ATAAAGATAG GGATAGTGAT 
TCGCTCAAGA CCCACAATAG CATCACTGCT CTTTTGGCAG CCGCCGCTGC CGAGGCCGCT 
GATCTCTCTC GGGAGTCGCC ACCTCCGTTG GTCGTTGGCA AATGCATGAA GATCGTCGAT 
GACACCAATA CCACCACCTG CTATACCAGT TCCAGCAGCG GGGAGGAGGA CGAAGACGAT 
CTGGATGCCT CCAACCGGGA GTACATATGC GAGGACGGCG AGAAGTTGGG ACCTCCGGAG 
CTGATAAATG GCCCCGGTCC CTCGGAGGCC TACTTCAACA TGTTCTGGCA CTCGAACATG 
CTGCCCACAA TCGGCGAGGT TGAAGAGGAG TTCTCCTCCC TGGAACCCCA ATCCCTAACA 
AACGGAAACC TTGTCAAATC GGAGGAGTCG AAGAAGTTAC CCAAAATGGA AGTAAACGCC 
GGTGGACTGG CCCAGCTGAC TGATGATAAG ATTGCTGACC ATGACGCAAG TGCAACCAGA 
GCGGATACTC GCGACGAAGT CACAACAAAC CAAACCCAGG AAATCAGCTC CAGCAGTTCG 
AGTTCTGTTA AAAGTCAAAA GACCACCACA ACCACTCGAG TGACCACCAC CAAGACTAGT 
TCCAGTTCCA GTTCCTCCAG TTGCTCATCC ACTGTTATCC CAGATGTTGC CTTCAAGCTG 
CAACCCTATG AGGAAAGGGA CGATATACTC TCGGAACCCA TTACCACCAT CCACGAAGAA 
CGACGTGTGC TAAGTGAGCA GAAGACCCTC AGTGAATCGA GGACTCGCGA TGCATTAACC 
GGCGAAGAGC AGCTGGTAAC TAGTGCCAAT TCGAAGTCGA GCAGTGCCCG CTTCAAGAAG 
ATCAGCAGCA ACGACAACCT ACTCGACCTT GGCGACGAGC AGGACATGCA GGAGCAGCCG 
TTGACCGCCA CGATCACCCA GGAGTCGAGT CTGAGGGAGC GGTTGGAGTG TCCTCAGGAT 
AACCAGAGTA TCGACAGGGG TACTCTGACC CTATCCGAGC GGGGAAAGCA GCTGAGCGAG 
AAAGAAGGCC TCACCACCTA CACGGAATGT GAGACGAGTG AAAAGGAGTC CTACCTAGAG 
GGCCAACAAG TCCTCAACGG CAAAGGCGGT TTCGTTAGCC AGGATGAGAA TAGTGACCAA 
CCAACTTACG AACGAGAGGT CAGCGAGACA CTAACGATCG TCAACGATGG CGAAAAGCAG 
GTCACCAAGA AATTGGAACA GAACAAGGAA ATTGCTGGCA AAAAGGGTGC CAAGCTCCTT 
AAGAAGACCG ATGAGGAGCG ACGTCTTGAG CAGGAGGCCC AAAAGCTGAT CGAAAGCTAT 
CAGAAAGTCA AGAAAGAAGC GGAGAAGCTG TACAAGCTGG AATTGGCCGA CGATGATCAG 
GGCTTTGATC TGAGTGCCTT CGAGCAGGCG GAGGAAGAGG ATAAGCCAGA AGTTTCTGAG 
GAAATCAAAG AAGTGAAGGA TTCTCAGCCA GTAAAGGTCG ATATTCAAAT TGAAGAAGAG 
GTTAAAACAC CTAATCAAGT CATAGGGGAA GTCAAAGTGG AAGATGTGAA TAAGGAAATT 
AAAGAGACGG TACAAGTCTC TCAACAGGAA ATTAAGAAAG AGGTCAAGGT CTCACACCAA 
GAAACAAAAG AAACCTTTAA GAGTGAAGAA AAAGATGAGA GTAATGAACT CAAAATAGTT 
AAGGAGGAGG TCAAAGTAAC ACATCAGGAT CTTAGGGAAC AGATCCAGGA GATCGAGGCT 
GTCAAAGTGG TGCACAAAGA AGTAAAAGTT ATTAAGGAAA GTGCTAAAGT CACTACGACC 
ACGAGTCACA AGCAAGCAAC TAAAGAAGTA ATCATCTCGA AAGAAGATCA CAAAAATGAT 
AGCGCACTCC CTGCCGTGTC AACCACAAAA GTAGAGATCA TCTCACCTCC CCTGGAAGAC 
GACTTGGGTT ATGTCCTCCA CAAGCACATT ATTTTTCCCC AGGAAAAGGT CAAGACGGCT 
CCTACGCCAC TGCCAAAACC GAAACCCAAA CCTCCAGTGC CCACGAACAA ACCCAAAATG 
CCGGTCAATT TGACCAAACC GAAATCAGCA CCACCACCGG TGCCCAGTAA GAGGAGTGAG 
CTATCCGGAT CCGGTGGCAA GCCCACACCC ACCCAAAGAC GCGGCAGCCT GGAGACCGCC 
CAGCCTCCAA AACCACTAGA ACGTATAATT GTCGGTGTGG AGCAGCGGGA TGAGCCAGAA 
AAGCCCCAGC CAATTATCAA CTTGGCCAGC GTGGATCTGC CAAATGTGGC GCCCAACAGC 
AAGCAAGCGA CAGTGGAAAC GACTCCCGTG GTCGAAATGC CACCGGATGA GCTCCAATTC 
GAGAGTCACC AACTGCCGGA TGACGGTGAA AAGGAAAAGG ACTTGCACCA GGAGTCAAAT 
GACCTGCTCA GCGGGTCGTC ATCGTCGTCG GTAACCACGC CCATAACAAC GCCAATTTTG 
GTATCTGCCA CCTCGTCGAT GGACTCGGTG CAAAGTGTCA TCGAGGTCGT CAATGGCATG 
CCCATCATCA GCAATCAGCA AACTGACGAC GACGACATTG ATGAAGTTGC AGCGTATGAC 
GATGGGATGC ATTTGGAGGC CGAAGGTACG AAGGATGTTC GTCCCGTCCA GGAGGTGACA 
ACGTTGACGC TCGACAGGGA ACACGAATCA GATCTTGGTA CCCTCAGTAA CCACCACAGC 
AATATAAGCA GCATTAGCGG CATTAACGCC AGTAGCAACA TCCCGGCAGC AACTTTAATC 
AGCAGCAGCA TTAAGAAGAC ATCCACTGAG GCGGCAACCA CAAAACCTGC TGCACCCCAG 
ATCCCCGGCA AAATGGAGCT GCTGAGCAGC AACACAGGAA CAACAACGAC AACGACGACA 
GCAACGCACC ACCTGCATCA GGCAACCACC ACTAAACAGC TGTTGGTGCA GGATTACCTC 
AGCTACGCCT CACCACCCAC ATATTCCCGC CTGCCGCCCG ATGGTCATGA ATTTCCACCC 
AATTTCAGCG AACCACTGAT CATGCACAGC CACCCACTAA AGGTCACCAC CGAGTTGAGT 
TACGAGATAC AGAAAGGCGG AAAGGATGAG TCCTCTGCAC CACCGCTTCC CAAAACGGGG 
CCACCAGCCA CAGTTCCCAG GAAAGTATAT CGCCAGGATT TGGTCATCAA CGTAGAGCCA 
GCACCCAGTT TAACCAGGGA CTATCAGAGA TCTCTGAGTG GTGGTACTCC CCGGAAGCCT 
AGCGATTGGC GCAAGGATGA AAAGTCGGAG AAATCAGTGC GCGACAAGAT TGCCATGTTC 
TCGTCAAACA ACGAACTGGA TGCCATACCG CCAGCTCCGG CAACAGCCCC CATTTCAAGC 
TCCTTCACCC GGAAGCCCTT GAATAGGAGC AGTGAAAATC TTCTGGACAG TTGCTCATCA 
TCCTCGGCTC CTTCGCTGAA AACTCGCGCG ATGAGCGTGG AAAATTTGAA TGATGTTCAG 
CGGCAATATC AGTTGGCCAA ACAGCTGCCC CAGTTGCACG TGGCCGACTC TATGTATTCC 
CTGAACACAG CCACGCCCAC ACCGAGCTAC GCCTCTTTGC CGAGAAGAAG CCACGGGGGA 
TCTTATTCTT CTGGAGTGGA GAGAAGGATC AGTTTCTCGG GAGAAGGCGG AGATGCGGCC 
AACCGAAAGG CAGCCATTAC AAATATCCTA GAGCAAAGAA GAAGAAGTTT GTCGAAACTG 
CGAGGATTGG TCATACCAGA GAGACCCCAA CTGCTGGAAC CCATCCTGGA TCTGCCGGAG 
ATCAAAAGCC AGGTAAAAGC AGCCAGCGGA GAGGATAGTA CGGATAGCGG ATTAGGCGAA 
AGCCACCGCA GTACGGTGAA TAGGAATTGC CAATTGGGTG CAGGAGGTGC CGCAAATAAC 
TATCGCAGCA TTTTAAATAC CAATCAAAGA AGACCCCTGG AGCAACAGCT CTCTCAACCA 
CCAGCCAAAC CACCCAGGAC ATCGTTGACA CCACTTCAGC CGAGAAGCAT GATGATACCA 
CCACCACCAC CTCCTTTAGA TCAGGAAAGT GATACGGATT CGGTGTTTTC GCACACTGCT 
AGGGTTGCCA CTCCGCCGGA GAAGTTTGCA CTAACCAGAA CCCTCAGCTC CGAAACAAAC 
ACTTCGATAG CTAGTTCAAA CACCTCTACT CTAACATCAG GATCTTCTGC CGGATCTCAA 
GCCAGTTGCA GTTCGCTGGG CAGCACACCA GCGGTGGATC TAACCAGGCG GGTTCTCAAG 
AGCCAGGTGA TCAATGGCGA AGCCGTTGCG CTCTCCAGTC GCAAAAGTAT TCTGGCCTCG 
GCCAAATGTC GCAGTGCCAA GAGTCGAGGT CAAGAGGAGG ATAACGACAG CACCGATGGT 
GAAGCCTGTT CCCTGGCCAA TCGTAGAATG AAGCCGATTT CCAGCTATAA GCTCCAGCAG 
CAACAGATCC AACTGGGCAA ACAACTGGTA GTGGATAAGC TGATCAATGT GGCCGCTTAT 
GTGGAACTAA CCTCGGATAC GGACGACAGC AGCCGTAGAT CTGATACGCC TGCCAAGATC 
AGTGCCATGT TCATAGACGA GGAGCGCAAG GCCAGCTTCA AAGGAGATCC TAACCAGCAG 
GCTAAGGTCA AAGTGGAGCA GGTCAAACCT ATGGTCCTCC CAATGCTGCT ACCATCTGCG 
AAAAGGGAAC CGCTGAAGCA ACAGACCACC GCCGAACTGC GCGAAAAGTT CGAAAGGAGT 
GCTGCAGCCC AAGCTCAAAC CCAAAACCAC TCGCCCGTAA TCCATAAGGT CACCCAGAAG 
CCGCATCATG AGCGATTCTC CTCGCTGGAC TCCCTGGCGT CCAGTTCCTC CGGCGTTAGT 
TCCACCACCC AAAACGTGAG CACCACCCAG GAAACGGCCA CCGAATTCGG CAGCTTTTCC 
TCGCTGGGTA GCAACCAGAG TTTGATCACC GCCCAGGATG TCCAGCAGAT AGTCGAGGAG 
GCTGATCCTC CACTAAAGAC CCCAGAAGCG TTCATCATCG TCCTGCAGAG GGAAAATCCC 
GAGAGCAGCA TCGGTATCAC TTTGGCCGGC GGTTCTGATT ATGAGGCTAA GGAAATTACG 
ATCCATAAGA TCCTGAGCAA CACGCCAGCT GCCAAGGATG GACGTCTAAA GAAGGGTGAT 
CGAATCCTTG CCGTCAATGG AATGAGCATG CGCGGACTGA CGCACCGCGA ATCCATCAGT 
GTGCTTAAGA CCCCCCGACC TGAGGTCGTG CTAGTGGTGA CCCGCTCCGA GTCGCTGGTG 
GTGAAGGCGC TGACCAAGAA ACGATCCTCC CTGGGATCAC TTAGCTCGCT CAATGAGAAA 
CCCACGGAGC TGGACTATGA ACGCAAGAGG AACTACCACA AGGCCTCCCG ATCTCTGGAC 
TTGGATCTTG ATCTGGTGTC CAATGAGGCT GGTGAATCGC CAGTGGCTAC TACTCCCAGC 
ACGGGATCCG TAAGTCCACC GCAGCCGGCG AGCTTGCACG ACGAGGATGC GGAGGCCACC 
ATCGCAGGTA TCCGGGCCAG GAGGCAGTTG TCCCGCGGAG ATGCCGCCAA GCTTAGCACG 
AGCGAGCTCC TGGAACGGGC GGCAGAGGCC AGAAATGCCA TCGCAGCTGA GATTCGTGCA 
CAAGCGGAGG ATGCGGCGGC CAGTGGAGGT GGAGCTCGTT GTGTGGAGAT TGTTAAGGAC 
AGCTGTGGAC TGGGTTTCTC CATCGAAGGA GGCTTTGATT CGCCGCTGGG AAATCGTCCC 
CTTATTGTCA AAAAGGTGTT CATGGGTGGT GCCGCCCAGA AGACCAACCA GGTGCGCAAT 
GGCGACGAAA TCCTTAGCAT CAATGGTGCC TCCACGTCGC GGATGACACG TGTGGATGCC 
TGGAACTATA TGAAGCAACT GCCGCTGGGA CCGGTCAAGA TTTGTTTCGC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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bbg cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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