RasGAP1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	RasGAP1
Entrez Gene ID	39158
Full Name	Ras GTPase activating protein 1
Synonyms	CG6721,Dmel\CG6721,FBpp0076097,GAP,GAP1,Gap,Gap 1,Gap1,Ras-GAP,RasGAP,RasGap1,gap,gap1,mip,rI533,rasGAP,sxt
General protein information	Preferred Names Ras GTPase activating protein 1 Names CG6721 gene product from transcript CG6721-RB CG6721-PA CG6721-PB CG6721-PC GTPase activating protein 1 GTPase activating protein1 GTPase-activating protein 1 RasGAP1-PA RasGAP1-PB RasGAP1-PC more inner photoreceptors seven extra sextra
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 67C10-67C11

mRNA and Protein(s)

mRNA	Protein	Name
NM_001274735.1	NP_001261664.1	Ras GTPase activating protein 1, isoform C
NM_168382.2	NP_729562.1	Ras GTPase activating protein 1, isoform B
NM_079290.3	NP_524014.2	Ras GTPase activating protein 1, isoform A

Pathways from BioSystems

Homologies

Homo sapiens (human)	RASA3	NP_031394.2
Gallus gallus (chicken)	RASA3	NP_001012843.1
Danio rerio (zebrafish)	rasa3	XP_002660361.1
Pan troglodytes (chimpanzee)	RASA3	XP_509751.3
Mus musculus (house mouse)	Rasa3	NP_033051.2
Drosophila melanogaster (fruit fly)	Gap1	NP_001261664.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002261	XP_307920.5
	RASA3	XP_001088279.2
Rattus norvegicus (Norway rat)	Rasa3	NP_113762.1
Canis lupus familiaris (dog)	RASA3	XP_005634195.1
Bos taurus (cattle)	RASA3	NP_777101.2
Caenorhabditis elegans (roundworm)	gap-1	NP_508404.1
Xenopus tropicalis (tropical clawed frog)	rasa3	XP_002937911.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the Ras/MEK/MAPK pathway regulates regional-fate specification via HSPGs during development
Title: Ras/MEK/MAPK-mediated regulation of heparin sulphate proteoglycans promotes retinal fate in the Drosophila eye-antennal disc.

the PlexA GAP domain is phosphorylated by the cAMP-dependent protein kinase (PKA). This PlexA phosphorylation generates a specific binding site for 14-3-3epsilon, a phospho-binding protein that we find to be necessary for axon guidance
Title: 14-3-3? couples protein kinase A to semaphorin signaling and silences plexin RasGAP-mediated axonal repulsion.

Neurofibromatosis type 1 (NF1) is among the most common genetic disorders of humans and is caused by loss of neurofibromin, a large and highly conserved protein whose only known function is to serve as a GTPase-Activating Protein (GAP) for Ras.
Title: Reduced growth of Drosophila neurofibromatosis 1 mutants reflects a non-cell-autonomous requirement for GTPase-Activating Protein activity in larval neurons.

The following RasGAP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RasGAP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa18120	NM_079290.3 Latest version!	Drosophila melanogaster Ras GTPase activating protein 1 (RasGAP1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa18120	NM_168382.2 Latest version!	Drosophila melanogaster Ras GTPase activating protein 1 (RasGAP1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa20819	NM_001274735.1 Latest version!	Drosophila melanogaster Ras GTPase activating protein 1 (RasGAP1), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RasGAP1 ( NM_168382.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729562.1 Drosophila melanogaster Ras GTPase activating protein 1 (RasGAP1), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa18120
Clone ID Related Accession (Same CDS sequence)	NM_168382.2 , NM_079290.3
Accession Version	NM_168382.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ras GTPase activating protein 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168382.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGCTGA AGAAGAAGCG CTATATGTTC GACGAGCGAG ACGACAATAA TATTAACTCG 
CCCGTCGCCG TCGAGCCAAG CAGCAACAAC AACAAAATGG CCGATACACG CGAAGTGCGC 
ATTGAAGAGC AATTAAAAGT GAAAATTGGC GAGGCAAAGA ATCTGAGCAG TCGCAATGCG 
GCCAACACTA GCTGCAGCAC ACAGGGCACT CGCGATGTCT ACTGCACAAT TGCGTTGGAC 
CAGGAGGAGA TCTGCCGCAC ACCCACCATC GAACGCACAC TGACGCCGTT CTTCGGCGAG 
GAGCACCAGT TCAAGATTCC GCGCCGCTTC CGCTACCTGA CCATCTATCT GTGGGATCGT 
GATATGAAGC AGGATAAACC CATTGGCAAG ATAGCGATCA AGCGGGAGGA GCTGCACATG 
TACAATCACA AGGATCACTG GTTCTCATTG AGGCCAGTCG ACCAAGACTC CGAGGTTCAG 
GGCATGGTTA ATGTGGAGGT GGCATTTACG GAAGCCCAAC AGACCCAGTC GCTCTCCGAG 
GGCATAGATC TGGGACAGCA CACATTGCGG CATCATCAGA ACCTGCCACA CCACAGCCAC 
CAGCAGCGGG CGCATCTAAA CGATTACAAG GAGAACAGTG AACTGAGTAA CATACAGCGG 
GCATCAGCAG CTGCAGCGAG TTCCTCATCG GCAGCCATGA CACTCAAGAC ACGAGCGGCC 
GGTCTATTTG GTCACGTGCA TCATCCGCCA TCGCAGACTC AGCACTTTCC GATCATCAAT 
ACCACATCCA CGTCGTCGGA TCAGTTGTCC AATTGGAAAT CGCATGGGAG ATTCGTTGGT 
GTGACCATCA AAGTACCAGC CTGCGTGGAC CTGGCCAAGA AGCAGGGCAC GTGCGATCCA 
TTTGTGGTCT GTACCGCCCA CTATAGCAAC AAGCACCAGG TGACGCGACG AACTAAGCAG 
CGCAAAAAGA CCGTGGATCC GGAGTTTGAC GAGGCCATGT ACTTTGATCT TCACATCGAT 
GCGGATGCGG GTAGCACAAA TACCACTGGA AGTAACAAGA GTGCCGGTTC CCTGGAATCA 
TCAGCGAACA AGGGCTATTC TATATACCCG GTGGGCGGTG CGGATCTGGT GGAGATTGTG 
GTGAGCGTGT GGCACGACGC ACATGGAGCC ATGTCCGATA AAGTGTTCCT TGGTGAAGTG 
CGGCTACCAA TGCTCAATAA GCAGGAGCAG CAGGCAGTTA ATCCATCGGC TTGGTACTAT 
CTACAGCCCC GCTCCATGAC GCACAGTTCC CGCTCGCTGA ACGCCACGCC CCGCTCATGT 
GCGACGCCGC CGGGAACGAG ATTAAGCGTG GACTCGACCA TTGGATCACT GCGCTTAAAT 
CTAAACTACA CCGCCGATCA TGTCTTCCCG CTGGCCACAT ACGACGATCT TATGAATCTG 
CTCCTGGAAT CGGTTGATCA GCGACCCATC ACCGTGTCGG CTGTGTCGAT CCTAGGCGAG 
CTGGTCTCGG GCAAGACGGA GGTGGCCCAG CCGCTGGTGC GACTCTTTAC GCACACGGAG 
CGCATTGCGC CGATCATCAA GGCGCTGGCT GACCACGAGA TCTCCCATCT GACAGATCCC 
ACCACTATCT TCCGGGGTAA CACCTTGGTC TCTAAGATGA TGGATGAGGC AATGCGATTG 
TCTGGACTGC ACTACCTACA TCAGACATTG CGTCCGGTTT TGTCGCAGAT CGTTGCCGAA 
AAGAAACCAT GTGAGATCGA TCCCAGCAAG ATTAAAGATC GCTCTGCGGT GGACACGAAT 
CTGCACAATC TGCAAGATTA TGTGGAGCGG GTTTTTGAGG CAATCACCAA GAGCGCTGAT 
CGCTGTCCGA AAGTTCTGTG TCAGATCTTC CACGACCTTC GCGAGTGTGC GGGTGAGCAT 
TTCCCCAGCA ATCGAGAAGT GCGCTACTCC GTGGTGTCCG GCTTTATCTT CCTGCGCTTC 
TTTGCACCCG CTATCCTGGG ACCAAAGCTC TTCGATCTGA CCACCGAGCG ACTGGATGCC 
CAAACGAGTC GCACGCTGAC TCTCATCTCC AAGACGATCC AATCGCTGGG TAATCTGGTC 
AGCTCCCGGT CGTCGCAACA GACTTGCAAG GAGGAGTTCA CCGTCGAGCT GTACAAGAAG 
TTCTGCACGG AACAGCACGT GGATGCGGTT AAGCACTTTC TGGAGGTGAT CTCGACGCCC 
AGTCATGCGA GCAGCAGCGT TCATCCGGCG GCGGCGGCGG CAACGCCACT GGAACCAGTT 
TTACTTAAAG AGGGCCTGAT GACCAAGTAT CCGACATCCC GGAAGCGTTT TGGCCGGCAG 
TTCAAACAAC GCCACTTCCG CCTCACCACC CACTCGCTCA GCTATGCGAA GTCGAAGGGC 
AAACAACCCA TCTGTGATAT ACCGCTCCAG GAGATCGCCA GCGTGGAGCA GCTGAAGGAC 
AAGAGTTTCA AGATGCAGAA CTGCTTTAAG ATTGTGCACA ACGATCGCTC GCTGATTGTG 
CAGACAACGA ACTGCGTGGA GGAGCGCGAG TGGTTCGACC TGCTGCACAA GATATGTCTG 
ATGAACTCCA TCCGGATGCA GTACTTCCAT CCCTCGGCCT TTGTAAGTGG ATTCTACAGC 
TGCTGCGGCC GGTCGGATGA GAATTCACCG GGCTGTAAGA AGGTGTTGGA CAAGACGATG 
GACTACTTCC AGATGGATTT AGTAACGGCG CTGGATCCAG CGCTCGATCT GCAGCGCATC 
CACACGCTGA TCATGTCTAA CATGAGTGTG CTGGAGTCAC TGCTAGATCC GCTCACCTAT 
CACCAGTCTC TGTCGCAGAC GCAACACCAA CAGCACAATC CTCTGGTGCC GCTGGCCACC 
GATCTGCAGA AGCATTCACC ACAGGCCTTC GCTGAGTTCA AGCGAACGAT TGAGAAGCTG 
AGAGAGAAGG CCTATGCCAT CGATAGAGAT CATCGGGACT ACAAGCAGGG CATTACGCGG 
CAACTGAAAT ACGGCAGCAG ACAGGCACCC ATTGGCGATG ATAACTATTG GCATATGATG 
CGAGCCGCCG GCCAGTTGAA TCAGCAGCAT CACCAGCAGC AGCAACATCA GCAGCAACAG 
CAACAGCAGC AGCAGCAGCA ACTTCAGCAG TTCCAGCCGC AGCCAGTGCT GCCGCAAATG 
CAGAACGTTC GGGCGTATCC TTATCAGCCA GCTACGAGCA ACATGAACGC CTACTGTCTG 
CACAACATGC AGTATCAGCA GCAGAGATTG CCGTTCCATC AGCAGCAACA GCAGCACCAT 
CAGCAGCTCC AGCAACAGCA GTCGCAGTTC CAGCCCCTGC GCAGCCATCA ACTGCAGCGC 
CACAACAACA ATTTGAACAA CAATAACTGC GGCAATGGAT CCTCCTCCAG TCCATCATCG 
ACCACATCAA GTGTGGTGGC CGCACCGCCC AGCACCACAT CGTCATCGCA GCCAGCTCCA 
CCGATTTATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729562.1
CDS	474..3965
Translation	MLLKKKRYMFDERDDNNINSPVAVEPSSNNNKMADTREVRIEEQLKVKIGEAKNLSSRNAANTSCSTQGTRDVYCTIALDQEEICRTPTIERTLTPFFGEEHQFKIPRRFRYLTIYLWDRDMKQDKPIGKIAIKREELHMYNHKDHWFSLRPVDQDSEVQGMVNVEVAFTEAQQTQSLSEGIDLGQHTLRHHQNLPHHSHQQRAHLNDYKENSELSNIQRASAAAASSSSAAMTLKTRAAGLFGHVHHPPSQTQHFPIINTTSTSSDQLSNWKSHGRFVGVTIKVPACVDLAKKQGTCDPFVVCTAHYSNKHQVTRRTKQRKKTVDPEFDEAMYFDLHIDADAGSTNTTGSNKSAGSLESSANKGYSIYPVGGADLVEIVVSVWHDAHGAMSDKVFLGEVRLPMLNKQEQQAVNPSAWYYLQPRSMTHSSRSLNATPRSCATPPGTRLSVDSTIGSLRLNLNYTADHVFPLATYDDLMNLLLESVDQRPITVSAVSILGELVSGKTEVAQPLVRLFTHTERIAPIIKALADHEISHLTDPTTIFRGNTLVSKMMDEAMRLSGLHYLHQTLRPVLSQIVAEKKPCEIDPSKIKDRSAVDTNLHNLQDYVERVFEAITKSADRCPKVLCQIFHDLRECAGEHFPSNREVRYSVVSGFIFLRFFAPAILGPKLFDLTTERLDAQTSRTLTLISKTIQSLGNLVSSRSSQQTCKEEFTVELYKKFCTEQHVDAVKHFLEVISTPSHASSSVHPAAAAATPLEPVLLKEGLMTKYPTSRKRFGRQFKQRHFRLTTHSLSYAKSKGKQPICDIPLQEIASVEQLKDKSFKMQNCFKIVHNDRSLIVQTTNCVEEREWFDLLHKICLMNSIRMQYFHPSAFVSGFYSCCGRSDENSPGCKKVLDKTMDYFQMDLVTALDPALDLQRIHTLIMSNMSVLESLLDPLTYHQSLSQTQHQQHNPLVPLATDLQKHSPQAFAEFKRTIEKLREKAYAIDRDHRDYKQGITRQLKYGSRQAPIGDDNYWHMMRAAGQLNQQHHQQQQHQQQQQQQQQQQLQQFQPQPVLPQMQNVRAYPYQPATSNMNAYCLHNMQYQQQRLPFHQQQQQHHQQLQQQQSQFQPLRSHQLQRHNNNLNNNNCGNGSSSSPSSTTSSVVAAPPSTTSSSQPAPPIY

Target ORF information:

RefSeq Version	NM_168382.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ras GTPase activating protein 1 (RasGAP1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168382.2

ORF Insert Sequence:

ATGCTGCTGA AGAAGAAGCG CTATATGTTC GACGAGCGAG ACGACAATAA TATTAACTCG 
CCCGTCGCCG TCGAGCCAAG CAGCAACAAC AACAAAATGG CCGATACACG CGAAGTGCGC 
ATTGAAGAGC AATTAAAAGT GAAAATTGGC GAGGCAAAGA ATCTGAGCAG TCGCAATGCG 
GCCAACACTA GCTGCAGCAC ACAGGGCACT CGCGATGTCT ACTGCACAAT TGCGTTGGAC 
CAGGAGGAGA TCTGCCGCAC ACCCACCATC GAACGCACAC TGACGCCGTT CTTCGGCGAG 
GAGCACCAGT TCAAGATTCC GCGCCGCTTC CGCTACCTGA CCATCTATCT GTGGGATCGT 
GATATGAAGC AGGATAAACC CATTGGCAAG ATAGCGATCA AGCGGGAGGA GCTGCACATG 
TACAATCACA AGGATCACTG GTTCTCATTG AGGCCAGTCG ACCAAGACTC CGAGGTTCAG 
GGCATGGTTA ATGTGGAGGT GGCATTTACG GAAGCCCAAC AGACCCAGTC GCTCTCCGAG 
GGCATAGATC TGGGACAGCA CACATTGCGG CATCATCAGA ACCTGCCACA CCACAGCCAC 
CAGCAGCGGG CGCATCTAAA CGATTACAAG GAGAACAGTG AACTGAGTAA CATACAGCGG 
GCATCAGCAG CTGCAGCGAG TTCCTCATCG GCAGCCATGA CACTCAAGAC ACGAGCGGCC 
GGTCTATTTG GTCACGTGCA TCATCCGCCA TCGCAGACTC AGCACTTTCC GATCATCAAT 
ACCACATCCA CGTCGTCGGA TCAGTTGTCC AATTGGAAAT CGCATGGGAG ATTCGTTGGT 
GTGACCATCA AAGTACCAGC CTGCGTGGAC CTGGCCAAGA AGCAGGGCAC GTGCGATCCA 
TTTGTGGTCT GTACCGCCCA CTATAGCAAC AAGCACCAGG TGACGCGACG AACTAAGCAG 
CGCAAAAAGA CCGTGGATCC GGAGTTTGAC GAGGCCATGT ACTTTGATCT TCACATCGAT 
GCGGATGCGG GTAGCACAAA TACCACTGGA AGTAACAAGA GTGCCGGTTC CCTGGAATCA 
TCAGCGAACA AGGGCTATTC TATATACCCG GTGGGCGGTG CGGATCTGGT GGAGATTGTG 
GTGAGCGTGT GGCACGACGC ACATGGAGCC ATGTCCGATA AAGTGTTCCT TGGTGAAGTG 
CGGCTACCAA TGCTCAATAA GCAGGAGCAG CAGGCAGTTA ATCCATCGGC TTGGTACTAT 
CTACAGCCCC GCTCCATGAC GCACAGTTCC CGCTCGCTGA ACGCCACGCC CCGCTCATGT 
GCGACGCCGC CGGGAACGAG ATTAAGCGTG GACTCGACCA TTGGATCACT GCGCTTAAAT 
CTAAACTACA CCGCCGATCA TGTCTTCCCG CTGGCCACAT ACGACGATCT TATGAATCTG 
CTCCTGGAAT CGGTTGATCA GCGACCCATC ACCGTGTCGG CTGTGTCGAT CCTAGGCGAG 
CTGGTCTCGG GCAAGACGGA GGTGGCCCAG CCGCTGGTGC GACTCTTTAC GCACACGGAG 
CGCATTGCGC CGATCATCAA GGCGCTGGCT GACCACGAGA TCTCCCATCT GACAGATCCC 
ACCACTATCT TCCGGGGTAA CACCTTGGTC TCTAAGATGA TGGATGAGGC AATGCGATTG 
TCTGGACTGC ACTACCTACA TCAGACATTG CGTCCGGTTT TGTCGCAGAT CGTTGCCGAA 
AAGAAACCAT GTGAGATCGA TCCCAGCAAG ATTAAAGATC GCTCTGCGGT GGACACGAAT 
CTGCACAATC TGCAAGATTA TGTGGAGCGG GTTTTTGAGG CAATCACCAA GAGCGCTGAT 
CGCTGTCCGA AAGTTCTGTG TCAGATCTTC CACGACCTTC GCGAGTGTGC GGGTGAGCAT 
TTCCCCAGCA ATCGAGAAGT GCGCTACTCC GTGGTGTCCG GCTTTATCTT CCTGCGCTTC 
TTTGCACCCG CTATCCTGGG ACCAAAGCTC TTCGATCTGA CCACCGAGCG ACTGGATGCC 
CAAACGAGTC GCACGCTGAC TCTCATCTCC AAGACGATCC AATCGCTGGG TAATCTGGTC 
AGCTCCCGGT CGTCGCAACA GACTTGCAAG GAGGAGTTCA CCGTCGAGCT GTACAAGAAG 
TTCTGCACGG AACAGCACGT GGATGCGGTT AAGCACTTTC TGGAGGTGAT CTCGACGCCC 
AGTCATGCGA GCAGCAGCGT TCATCCGGCG GCGGCGGCGG CAACGCCACT GGAACCAGTT 
TTACTTAAAG AGGGCCTGAT GACCAAGTAT CCGACATCCC GGAAGCGTTT TGGCCGGCAG 
TTCAAACAAC GCCACTTCCG CCTCACCACC CACTCGCTCA GCTATGCGAA GTCGAAGGGC 
AAACAACCCA TCTGTGATAT ACCGCTCCAG GAGATCGCCA GCGTGGAGCA GCTGAAGGAC 
AAGAGTTTCA AGATGCAGAA CTGCTTTAAG ATTGTGCACA ACGATCGCTC GCTGATTGTG 
CAGACAACGA ACTGCGTGGA GGAGCGCGAG TGGTTCGACC TGCTGCACAA GATATGTCTG 
ATGAACTCCA TCCGGATGCA GTACTTCCAT CCCTCGGCCT TTGTAAGTGG ATTCTACAGC 
TGCTGCGGCC GGTCGGATGA GAATTCACCG GGCTGTAAGA AGGTGTTGGA CAAGACGATG 
GACTACTTCC AGATGGATTT AGTAACGGCG CTGGATCCAG CGCTCGATCT GCAGCGCATC 
CACACGCTGA TCATGTCTAA CATGAGTGTG CTGGAGTCAC TGCTAGATCC GCTCACCTAT 
CACCAGTCTC TGTCGCAGAC GCAACACCAA CAGCACAATC CTCTGGTGCC GCTGGCCACC 
GATCTGCAGA AGCATTCACC ACAGGCCTTC GCTGAGTTCA AGCGAACGAT TGAGAAGCTG 
AGAGAGAAGG CCTATGCCAT CGATAGAGAT CATCGGGACT ACAAGCAGGG CATTACGCGG 
CAACTGAAAT ACGGCAGCAG ACAGGCACCC ATTGGCGATG ATAACTATTG GCATATGATG 
CGAGCCGCCG GCCAGTTGAA TCAGCAGCAT CACCAGCAGC AGCAACATCA GCAGCAACAG 
CAACAGCAGC AGCAGCAGCA ACTTCAGCAG TTCCAGCCGC AGCCAGTGCT GCCGCAAATG 
CAGAACGTTC GGGCGTATCC TTATCAGCCA GCTACGAGCA ACATGAACGC CTACTGTCTG 
CACAACATGC AGTATCAGCA GCAGAGATTG CCGTTCCATC AGCAGCAACA GCAGCACCAT 
CAGCAGCTCC AGCAACAGCA GTCGCAGTTC CAGCCCCTGC GCAGCCATCA ACTGCAGCGC 
CACAACAACA ATTTGAACAA CAATAACTGC GGCAATGGAT CCTCCTCCAG TCCATCATCG 
ACCACATCAA GTGTGGTGGC CGCACCGCCC AGCACCACAT CGTCATCGCA GCCAGCTCCA 
CCGATTTATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RasGAP1 ( NM_079290.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524014.2 Drosophila melanogaster Ras GTPase activating protein 1 (RasGAP1), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa18120
Clone ID Related Accession (Same CDS sequence)	NM_168382.2 , NM_079290.3
Accession Version	NM_079290.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ras GTPase activating protein 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079290.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGCTGA AGAAGAAGCG CTATATGTTC GACGAGCGAG ACGACAATAA TATTAACTCG 
CCCGTCGCCG TCGAGCCAAG CAGCAACAAC AACAAAATGG CCGATACACG CGAAGTGCGC 
ATTGAAGAGC AATTAAAAGT GAAAATTGGC GAGGCAAAGA ATCTGAGCAG TCGCAATGCG 
GCCAACACTA GCTGCAGCAC ACAGGGCACT CGCGATGTCT ACTGCACAAT TGCGTTGGAC 
CAGGAGGAGA TCTGCCGCAC ACCCACCATC GAACGCACAC TGACGCCGTT CTTCGGCGAG 
GAGCACCAGT TCAAGATTCC GCGCCGCTTC CGCTACCTGA CCATCTATCT GTGGGATCGT 
GATATGAAGC AGGATAAACC CATTGGCAAG ATAGCGATCA AGCGGGAGGA GCTGCACATG 
TACAATCACA AGGATCACTG GTTCTCATTG AGGCCAGTCG ACCAAGACTC CGAGGTTCAG 
GGCATGGTTA ATGTGGAGGT GGCATTTACG GAAGCCCAAC AGACCCAGTC GCTCTCCGAG 
GGCATAGATC TGGGACAGCA CACATTGCGG CATCATCAGA ACCTGCCACA CCACAGCCAC 
CAGCAGCGGG CGCATCTAAA CGATTACAAG GAGAACAGTG AACTGAGTAA CATACAGCGG 
GCATCAGCAG CTGCAGCGAG TTCCTCATCG GCAGCCATGA CACTCAAGAC ACGAGCGGCC 
GGTCTATTTG GTCACGTGCA TCATCCGCCA TCGCAGACTC AGCACTTTCC GATCATCAAT 
ACCACATCCA CGTCGTCGGA TCAGTTGTCC AATTGGAAAT CGCATGGGAG ATTCGTTGGT 
GTGACCATCA AAGTACCAGC CTGCGTGGAC CTGGCCAAGA AGCAGGGCAC GTGCGATCCA 
TTTGTGGTCT GTACCGCCCA CTATAGCAAC AAGCACCAGG TGACGCGACG AACTAAGCAG 
CGCAAAAAGA CCGTGGATCC GGAGTTTGAC GAGGCCATGT ACTTTGATCT TCACATCGAT 
GCGGATGCGG GTAGCACAAA TACCACTGGA AGTAACAAGA GTGCCGGTTC CCTGGAATCA 
TCAGCGAACA AGGGCTATTC TATATACCCG GTGGGCGGTG CGGATCTGGT GGAGATTGTG 
GTGAGCGTGT GGCACGACGC ACATGGAGCC ATGTCCGATA AAGTGTTCCT TGGTGAAGTG 
CGGCTACCAA TGCTCAATAA GCAGGAGCAG CAGGCAGTTA ATCCATCGGC TTGGTACTAT 
CTACAGCCCC GCTCCATGAC GCACAGTTCC CGCTCGCTGA ACGCCACGCC CCGCTCATGT 
GCGACGCCGC CGGGAACGAG ATTAAGCGTG GACTCGACCA TTGGATCACT GCGCTTAAAT 
CTAAACTACA CCGCCGATCA TGTCTTCCCG CTGGCCACAT ACGACGATCT TATGAATCTG 
CTCCTGGAAT CGGTTGATCA GCGACCCATC ACCGTGTCGG CTGTGTCGAT CCTAGGCGAG 
CTGGTCTCGG GCAAGACGGA GGTGGCCCAG CCGCTGGTGC GACTCTTTAC GCACACGGAG 
CGCATTGCGC CGATCATCAA GGCGCTGGCT GACCACGAGA TCTCCCATCT GACAGATCCC 
ACCACTATCT TCCGGGGTAA CACCTTGGTC TCTAAGATGA TGGATGAGGC AATGCGATTG 
TCTGGACTGC ACTACCTACA TCAGACATTG CGTCCGGTTT TGTCGCAGAT CGTTGCCGAA 
AAGAAACCAT GTGAGATCGA TCCCAGCAAG ATTAAAGATC GCTCTGCGGT GGACACGAAT 
CTGCACAATC TGCAAGATTA TGTGGAGCGG GTTTTTGAGG CAATCACCAA GAGCGCTGAT 
CGCTGTCCGA AAGTTCTGTG TCAGATCTTC CACGACCTTC GCGAGTGTGC GGGTGAGCAT 
TTCCCCAGCA ATCGAGAAGT GCGCTACTCC GTGGTGTCCG GCTTTATCTT CCTGCGCTTC 
TTTGCACCCG CTATCCTGGG ACCAAAGCTC TTCGATCTGA CCACCGAGCG ACTGGATGCC 
CAAACGAGTC GCACGCTGAC TCTCATCTCC AAGACGATCC AATCGCTGGG TAATCTGGTC 
AGCTCCCGGT CGTCGCAACA GACTTGCAAG GAGGAGTTCA CCGTCGAGCT GTACAAGAAG 
TTCTGCACGG AACAGCACGT GGATGCGGTT AAGCACTTTC TGGAGGTGAT CTCGACGCCC 
AGTCATGCGA GCAGCAGCGT TCATCCGGCG GCGGCGGCGG CAACGCCACT GGAACCAGTT 
TTACTTAAAG AGGGCCTGAT GACCAAGTAT CCGACATCCC GGAAGCGTTT TGGCCGGCAG 
TTCAAACAAC GCCACTTCCG CCTCACCACC CACTCGCTCA GCTATGCGAA GTCGAAGGGC 
AAACAACCCA TCTGTGATAT ACCGCTCCAG GAGATCGCCA GCGTGGAGCA GCTGAAGGAC 
AAGAGTTTCA AGATGCAGAA CTGCTTTAAG ATTGTGCACA ACGATCGCTC GCTGATTGTG 
CAGACAACGA ACTGCGTGGA GGAGCGCGAG TGGTTCGACC TGCTGCACAA GATATGTCTG 
ATGAACTCCA TCCGGATGCA GTACTTCCAT CCCTCGGCCT TTGTAAGTGG ATTCTACAGC 
TGCTGCGGCC GGTCGGATGA GAATTCACCG GGCTGTAAGA AGGTGTTGGA CAAGACGATG 
GACTACTTCC AGATGGATTT AGTAACGGCG CTGGATCCAG CGCTCGATCT GCAGCGCATC 
CACACGCTGA TCATGTCTAA CATGAGTGTG CTGGAGTCAC TGCTAGATCC GCTCACCTAT 
CACCAGTCTC TGTCGCAGAC GCAACACCAA CAGCACAATC CTCTGGTGCC GCTGGCCACC 
GATCTGCAGA AGCATTCACC ACAGGCCTTC GCTGAGTTCA AGCGAACGAT TGAGAAGCTG 
AGAGAGAAGG CCTATGCCAT CGATAGAGAT CATCGGGACT ACAAGCAGGG CATTACGCGG 
CAACTGAAAT ACGGCAGCAG ACAGGCACCC ATTGGCGATG ATAACTATTG GCATATGATG 
CGAGCCGCCG GCCAGTTGAA TCAGCAGCAT CACCAGCAGC AGCAACATCA GCAGCAACAG 
CAACAGCAGC AGCAGCAGCA ACTTCAGCAG TTCCAGCCGC AGCCAGTGCT GCCGCAAATG 
CAGAACGTTC GGGCGTATCC TTATCAGCCA GCTACGAGCA ACATGAACGC CTACTGTCTG 
CACAACATGC AGTATCAGCA GCAGAGATTG CCGTTCCATC AGCAGCAACA GCAGCACCAT 
CAGCAGCTCC AGCAACAGCA GTCGCAGTTC CAGCCCCTGC GCAGCCATCA ACTGCAGCGC 
CACAACAACA ATTTGAACAA CAATAACTGC GGCAATGGAT CCTCCTCCAG TCCATCATCG 
ACCACATCAA GTGTGGTGGC CGCACCGCCC AGCACCACAT CGTCATCGCA GCCAGCTCCA 
CCGATTTATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524014.2
CDS	474..3965
Translation	MLLKKKRYMFDERDDNNINSPVAVEPSSNNNKMADTREVRIEEQLKVKIGEAKNLSSRNAANTSCSTQGTRDVYCTIALDQEEICRTPTIERTLTPFFGEEHQFKIPRRFRYLTIYLWDRDMKQDKPIGKIAIKREELHMYNHKDHWFSLRPVDQDSEVQGMVNVEVAFTEAQQTQSLSEGIDLGQHTLRHHQNLPHHSHQQRAHLNDYKENSELSNIQRASAAAASSSSAAMTLKTRAAGLFGHVHHPPSQTQHFPIINTTSTSSDQLSNWKSHGRFVGVTIKVPACVDLAKKQGTCDPFVVCTAHYSNKHQVTRRTKQRKKTVDPEFDEAMYFDLHIDADAGSTNTTGSNKSAGSLESSANKGYSIYPVGGADLVEIVVSVWHDAHGAMSDKVFLGEVRLPMLNKQEQQAVNPSAWYYLQPRSMTHSSRSLNATPRSCATPPGTRLSVDSTIGSLRLNLNYTADHVFPLATYDDLMNLLLESVDQRPITVSAVSILGELVSGKTEVAQPLVRLFTHTERIAPIIKALADHEISHLTDPTTIFRGNTLVSKMMDEAMRLSGLHYLHQTLRPVLSQIVAEKKPCEIDPSKIKDRSAVDTNLHNLQDYVERVFEAITKSADRCPKVLCQIFHDLRECAGEHFPSNREVRYSVVSGFIFLRFFAPAILGPKLFDLTTERLDAQTSRTLTLISKTIQSLGNLVSSRSSQQTCKEEFTVELYKKFCTEQHVDAVKHFLEVISTPSHASSSVHPAAAAATPLEPVLLKEGLMTKYPTSRKRFGRQFKQRHFRLTTHSLSYAKSKGKQPICDIPLQEIASVEQLKDKSFKMQNCFKIVHNDRSLIVQTTNCVEEREWFDLLHKICLMNSIRMQYFHPSAFVSGFYSCCGRSDENSPGCKKVLDKTMDYFQMDLVTALDPALDLQRIHTLIMSNMSVLESLLDPLTYHQSLSQTQHQQHNPLVPLATDLQKHSPQAFAEFKRTIEKLREKAYAIDRDHRDYKQGITRQLKYGSRQAPIGDDNYWHMMRAAGQLNQQHHQQQQHQQQQQQQQQQQLQQFQPQPVLPQMQNVRAYPYQPATSNMNAYCLHNMQYQQQRLPFHQQQQQHHQQLQQQQSQFQPLRSHQLQRHNNNLNNNNCGNGSSSSPSSTTSSVVAAPPSTTSSSQPAPPIY

Target ORF information:

RefSeq Version	NM_079290.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ras GTPase activating protein 1 (RasGAP1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079290.3

ORF Insert Sequence:

ATGCTGCTGA AGAAGAAGCG CTATATGTTC GACGAGCGAG ACGACAATAA TATTAACTCG 
CCCGTCGCCG TCGAGCCAAG CAGCAACAAC AACAAAATGG CCGATACACG CGAAGTGCGC 
ATTGAAGAGC AATTAAAAGT GAAAATTGGC GAGGCAAAGA ATCTGAGCAG TCGCAATGCG 
GCCAACACTA GCTGCAGCAC ACAGGGCACT CGCGATGTCT ACTGCACAAT TGCGTTGGAC 
CAGGAGGAGA TCTGCCGCAC ACCCACCATC GAACGCACAC TGACGCCGTT CTTCGGCGAG 
GAGCACCAGT TCAAGATTCC GCGCCGCTTC CGCTACCTGA CCATCTATCT GTGGGATCGT 
GATATGAAGC AGGATAAACC CATTGGCAAG ATAGCGATCA AGCGGGAGGA GCTGCACATG 
TACAATCACA AGGATCACTG GTTCTCATTG AGGCCAGTCG ACCAAGACTC CGAGGTTCAG 
GGCATGGTTA ATGTGGAGGT GGCATTTACG GAAGCCCAAC AGACCCAGTC GCTCTCCGAG 
GGCATAGATC TGGGACAGCA CACATTGCGG CATCATCAGA ACCTGCCACA CCACAGCCAC 
CAGCAGCGGG CGCATCTAAA CGATTACAAG GAGAACAGTG AACTGAGTAA CATACAGCGG 
GCATCAGCAG CTGCAGCGAG TTCCTCATCG GCAGCCATGA CACTCAAGAC ACGAGCGGCC 
GGTCTATTTG GTCACGTGCA TCATCCGCCA TCGCAGACTC AGCACTTTCC GATCATCAAT 
ACCACATCCA CGTCGTCGGA TCAGTTGTCC AATTGGAAAT CGCATGGGAG ATTCGTTGGT 
GTGACCATCA AAGTACCAGC CTGCGTGGAC CTGGCCAAGA AGCAGGGCAC GTGCGATCCA 
TTTGTGGTCT GTACCGCCCA CTATAGCAAC AAGCACCAGG TGACGCGACG AACTAAGCAG 
CGCAAAAAGA CCGTGGATCC GGAGTTTGAC GAGGCCATGT ACTTTGATCT TCACATCGAT 
GCGGATGCGG GTAGCACAAA TACCACTGGA AGTAACAAGA GTGCCGGTTC CCTGGAATCA 
TCAGCGAACA AGGGCTATTC TATATACCCG GTGGGCGGTG CGGATCTGGT GGAGATTGTG 
GTGAGCGTGT GGCACGACGC ACATGGAGCC ATGTCCGATA AAGTGTTCCT TGGTGAAGTG 
CGGCTACCAA TGCTCAATAA GCAGGAGCAG CAGGCAGTTA ATCCATCGGC TTGGTACTAT 
CTACAGCCCC GCTCCATGAC GCACAGTTCC CGCTCGCTGA ACGCCACGCC CCGCTCATGT 
GCGACGCCGC CGGGAACGAG ATTAAGCGTG GACTCGACCA TTGGATCACT GCGCTTAAAT 
CTAAACTACA CCGCCGATCA TGTCTTCCCG CTGGCCACAT ACGACGATCT TATGAATCTG 
CTCCTGGAAT CGGTTGATCA GCGACCCATC ACCGTGTCGG CTGTGTCGAT CCTAGGCGAG 
CTGGTCTCGG GCAAGACGGA GGTGGCCCAG CCGCTGGTGC GACTCTTTAC GCACACGGAG 
CGCATTGCGC CGATCATCAA GGCGCTGGCT GACCACGAGA TCTCCCATCT GACAGATCCC 
ACCACTATCT TCCGGGGTAA CACCTTGGTC TCTAAGATGA TGGATGAGGC AATGCGATTG 
TCTGGACTGC ACTACCTACA TCAGACATTG CGTCCGGTTT TGTCGCAGAT CGTTGCCGAA 
AAGAAACCAT GTGAGATCGA TCCCAGCAAG ATTAAAGATC GCTCTGCGGT GGACACGAAT 
CTGCACAATC TGCAAGATTA TGTGGAGCGG GTTTTTGAGG CAATCACCAA GAGCGCTGAT 
CGCTGTCCGA AAGTTCTGTG TCAGATCTTC CACGACCTTC GCGAGTGTGC GGGTGAGCAT 
TTCCCCAGCA ATCGAGAAGT GCGCTACTCC GTGGTGTCCG GCTTTATCTT CCTGCGCTTC 
TTTGCACCCG CTATCCTGGG ACCAAAGCTC TTCGATCTGA CCACCGAGCG ACTGGATGCC 
CAAACGAGTC GCACGCTGAC TCTCATCTCC AAGACGATCC AATCGCTGGG TAATCTGGTC 
AGCTCCCGGT CGTCGCAACA GACTTGCAAG GAGGAGTTCA CCGTCGAGCT GTACAAGAAG 
TTCTGCACGG AACAGCACGT GGATGCGGTT AAGCACTTTC TGGAGGTGAT CTCGACGCCC 
AGTCATGCGA GCAGCAGCGT TCATCCGGCG GCGGCGGCGG CAACGCCACT GGAACCAGTT 
TTACTTAAAG AGGGCCTGAT GACCAAGTAT CCGACATCCC GGAAGCGTTT TGGCCGGCAG 
TTCAAACAAC GCCACTTCCG CCTCACCACC CACTCGCTCA GCTATGCGAA GTCGAAGGGC 
AAACAACCCA TCTGTGATAT ACCGCTCCAG GAGATCGCCA GCGTGGAGCA GCTGAAGGAC 
AAGAGTTTCA AGATGCAGAA CTGCTTTAAG ATTGTGCACA ACGATCGCTC GCTGATTGTG 
CAGACAACGA ACTGCGTGGA GGAGCGCGAG TGGTTCGACC TGCTGCACAA GATATGTCTG 
ATGAACTCCA TCCGGATGCA GTACTTCCAT CCCTCGGCCT TTGTAAGTGG ATTCTACAGC 
TGCTGCGGCC GGTCGGATGA GAATTCACCG GGCTGTAAGA AGGTGTTGGA CAAGACGATG 
GACTACTTCC AGATGGATTT AGTAACGGCG CTGGATCCAG CGCTCGATCT GCAGCGCATC 
CACACGCTGA TCATGTCTAA CATGAGTGTG CTGGAGTCAC TGCTAGATCC GCTCACCTAT 
CACCAGTCTC TGTCGCAGAC GCAACACCAA CAGCACAATC CTCTGGTGCC GCTGGCCACC 
GATCTGCAGA AGCATTCACC ACAGGCCTTC GCTGAGTTCA AGCGAACGAT TGAGAAGCTG 
AGAGAGAAGG CCTATGCCAT CGATAGAGAT CATCGGGACT ACAAGCAGGG CATTACGCGG 
CAACTGAAAT ACGGCAGCAG ACAGGCACCC ATTGGCGATG ATAACTATTG GCATATGATG 
CGAGCCGCCG GCCAGTTGAA TCAGCAGCAT CACCAGCAGC AGCAACATCA GCAGCAACAG 
CAACAGCAGC AGCAGCAGCA ACTTCAGCAG TTCCAGCCGC AGCCAGTGCT GCCGCAAATG 
CAGAACGTTC GGGCGTATCC TTATCAGCCA GCTACGAGCA ACATGAACGC CTACTGTCTG 
CACAACATGC AGTATCAGCA GCAGAGATTG CCGTTCCATC AGCAGCAACA GCAGCACCAT 
CAGCAGCTCC AGCAACAGCA GTCGCAGTTC CAGCCCCTGC GCAGCCATCA ACTGCAGCGC 
CACAACAACA ATTTGAACAA CAATAACTGC GGCAATGGAT CCTCCTCCAG TCCATCATCG 
ACCACATCAA GTGTGGTGGC CGCACCGCCC AGCACCACAT CGTCATCGCA GCCAGCTCCA 
CCGATTTATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RasGAP1 ( NM_001274735.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261664.1 Drosophila melanogaster Ras GTPase activating protein 1 (RasGAP1), transcript variant C, mRNA.

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CloneID	OFa20819
Clone ID Related Accession (Same CDS sequence)	NM_001274735.1
Accession Version	NM_001274735.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ras GTPase activating protein 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGCTGA AGAAGAAGCG CTATATGTTC GACGAGCGAG ACGACAATAA TATTAACTCG 
CCCGTCGCCG TCGAGCCAAG CAGCAACAAC AACAAAATGG CCGATACACG CGAAGTGCGC 
ATTGAAGAGC AATTAAAAGT GAAAATTGGC GAGGCAAAGA ATCTGAGCAG TCGCAATGCG 
GCCAACACTA GCTGCAGCAC ACAGGGCACT CGCGATGTCT ACTGCACAAT TGCGTTGGAC 
CAGGAGGAGA TCTGCCGCAC ACCCACCATC GAACGCACAC TGACGCCGTT CTTCGGCGAG 
GAGCACCAGT TCAAGATTCC GCGCCGCTTC CGCTACCTGA CCATCTATCT GTGGGATCGT 
GATATGAAGC AGGATAAACC CATTGGCAAG ATAGCGATCA AGCGGGAGGA GCTGCACATG 
TACAATCACA AGGATCACTG GTTCTCATTG AGGCCAGTCG ACCAAGACTC CGAGGTTCAG 
GGCATGGTTA ATGTGGAGGT GGCATTTACG GAAGCCCAAC AGACCCAGTC GCTCTCCGAG 
GGCATAGATC TGGGACAGCA CACATTGCGG CATCATCAGA ACCTGCCACA CCACAGCCAC 
CAGCAGCGGG CGCATCTAAA CGATTACAAG GAGAACAGTG AACTGAGTAA CATACAGCGG 
GCATCAGCAG CTGCAGCGAG TTCCTCATCG GCAGCCATGA CACTCAAGAC ACGAGCGGCC 
GGTCTATTTG GTCACGTGCA TCATCCGCCA TCGCAGACTC AGCACTTTCC GATCATCAAT 
ACCACATCCA CGTCGTCGGA TCAGTTGTCC AATTGGAAAT CGCATGGGAG ATTCGTTGGT 
GTGACCATCA AAGTACCAGC CTGCGTGGAC CTGGCCAAGA AGCAGGGCAC GTGCGATCCA 
TTTGTGGTCT GTACCGCCCA CTATAGCAAC AAGCACCAGG TGACGCGACG AACTAAGCAG 
CGCAAAAAGA CCGTGGATCC GGAGTTTGAC GAGGCCATGT ACTTTGATCT TCACATCGAT 
GCGGATGCGG GTAGCACAAA TACCACTGGA AGTAACAAGA GTGCCGGTTC CCTGGAATCA 
TCAGCGAACA AGGGCTATTC TATATACCCG GTGGGCGGTG CGGATCTGGT GGAGATTGTG 
GTGAGCGTGT GGCACGACGC ACATGGAGCC ATGTCCGATA AAGTGTTCCT TGGTGAAGTG 
CGGCTACCAA TGCTCAATAA GCAGGAGCAG CAGGCAGTTA ATCCATCGGC TTGGTACTAT 
CTACAGCCCC GCTCCATGAC GCACAGTTCC CGCTCGCTGA ACGCCACGCC CCGCTCATGT 
GCGACGCCGC CGGGAACGAG ATTAAGCGTG GACTCGACCA TTGGATCACT GCGCTTAAAT 
CTAAACTACA CCGCCGATCA TGTCTTCCCG CTGGCCACAT ACGACGATCT TATGAATCTG 
CTCCTGGAAT CGGTTGATCA GCGACCCATC ACCGTGTCGG CTGTGTCGAT CCTAGGCGAG 
CTGGTCTCGG GCAAGACGGA GGTGGCCCAG CCGCTGGTGC GACTCTTTAC GCACACGGAG 
CGCATTGCGC CGATCATCAA GGCGCTGGCT GACCACGAGA TCTCCCATCT GACAGATCCC 
ACCACTATCT TCCGGGGTAA CACCTTGGTC TCTAAGATGA TGGATGAGGC AATGCGATTG 
TCTGGACTGC ACTACCTACA TCAGACATTG CGTCCGGTTT TGTCGCAGAT CGTTGCCGAA 
AAGAAACCAT GTGAGATCGA TCCCAGCAAG ATTAAAGATC GCTCTGCGGT GGACACGAAT 
CTGCACAATC TGCAAGATTA TGTGGAGCGG GTTTTTGAGG CAATCACCAA GAGCGCTGAT 
CGCTGTCCGA AAGTTCTGTG TCAGATCTTC CACGACCTTC GCGAGTGTGC GGGTGAGCAT 
TTCCCCAGCA ATCGAGAAGT GCGCTACTCC GTGGTGTCCG GCTTTATCTT CCTGCGCTTC 
TTTGCACCCG CTATCCTGGG ACCAAAGCTC TTCGATCTGA CCACCGAGCG ACTGGATGCC 
CAAACGAGTC GCACGCTGAC TCTCATCTCC AAGACGATCC AATCGCTGGG TAATCTGGTC 
AGCTCCCGGT CGTCGCAACA GACTTGCAAG GAGGAGTTCA CCGTCGAGCT GTACAAGAAG 
TTCTGCACGG AACAGCACGT GGATGCGGTT AAGCACTTTC TGGAGGTGAT CTCGACGCCC 
AGTCATGCGA GCAGCAGCGT TCATCCGGCG GCGGCGGCGG CAACGCCACT GGAACCAGTT 
TTACTTAAAG AGGGCCTGAT GACCAAGTAT CCGACATCCC GGAAGCGTTT TGGCCGGCAG 
TTCAAACAAC GCCACTTCCG CCTCACCACC CACTCGCTCA GCTATGCGAA GTCGAAGGGC 
AAACAACCCA TCTGTGATAT ACCGCTCCAG GAGATCGCCA GCGTGGAGCA GCTGAAGGAC 
AAGAGTTTCA AGATGCAGAA CTGCTTTAAG ATTGTGCACA ACGATCGCTC GCTGATTGTG 
CAGACAACGA ACTGCGTGGA GGAGCGCGAG TGGTTCGACC TGCTGCACAA GATATGTCTG 
ATGAACTCCA TCCGGATGCA GTACTTCCAT CCCTCGGCCT TTGTAAGTGG ATTCTACAGC 
TGCTGCGGCC GGTCGGATGA GAATTCACCG GGCTGTAAGA AGGTGTTGGA CAAGACGATG 
GACTACTTCC AGATGGATTT AGTAACGGCG CTGGATCCAG CGCTCGATCT GCAGCGCATC 
CACACGCTGA TCATGTCTAA CATGAGTGTG CTGGAGTCAC TGCTAGATCC GCTCACCTAT 
CACCAGTCTC TGTCGCAGAC GCAACACCAA CAGCACAATC CTCTGGTGCC GCTGGCCACC 
GATCTGCAGA AGCATTCACC ACAGGCCTTC GCTGAGTTCA AGCGAACGAT TGAGAAGCTG 
AGAGAGAAGG CCTATGCCAT CGATAGAGAT CATCGGGACT ACAAGCAGGG CATTACGCGG 
CAACTGAAAT ACGGCAGCAG ACAGGCACCC ATTGGCGATG ATAACTATTG GCATATGATG 
CGAGCCGCCG GCCAGTTGAA TCAGCAGCAT CACCAGCAGC AGCAACATCA GCAGCAACAG 
CAACAGCAGC AGCAGCAGCA ACTTCAGCAG TTCCAGCCGC AGCCAGTGCT GCCGCAAATG 
CAGAACGTTC GGGCGTATCC TTATCAGCCA GCTACGAGCA ACATGAACGC CTACTGTCTG 
CACAACATGC AGTATCAGCA GCAGAGATTG CCGTTCCATC AGCAGCAACA GCAGCACCAT 
CAGCAGCTCC AGCAACAGCA GTCGCAGTTC CAGCCCCTGC GCAGCCATCA ACTGCAGCGC 
CACAACAACA ATTTGAACAA CAATAACTGC GGCAATGGAT CCTCCTCCAG TCCATCATCG 
ACCACATCAA GTGTGGTGGC CGCACCGCCC AGCACCACAT CGTCATCGCA GCCAGCTCCA 
CCGATTTATT AGCGTTCAAG GCTGTGCGTA TTCGCTTTGC CGCGGGCCAT TATCACGACC 
AGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261664.1
CDS	474..4019
Translation	MLLKKKRYMFDERDDNNINSPVAVEPSSNNNKMADTREVRIEEQLKVKIGEAKNLSSRNAANTSCSTQGTRDVYCTIALDQEEICRTPTIERTLTPFFGEEHQFKIPRRFRYLTIYLWDRDMKQDKPIGKIAIKREELHMYNHKDHWFSLRPVDQDSEVQGMVNVEVAFTEAQQTQSLSEGIDLGQHTLRHHQNLPHHSHQQRAHLNDYKENSELSNIQRASAAAASSSSAAMTLKTRAAGLFGHVHHPPSQTQHFPIINTTSTSSDQLSNWKSHGRFVGVTIKVPACVDLAKKQGTCDPFVVCTAHYSNKHQVTRRTKQRKKTVDPEFDEAMYFDLHIDADAGSTNTTGSNKSAGSLESSANKGYSIYPVGGADLVEIVVSVWHDAHGAMSDKVFLGEVRLPMLNKQEQQAVNPSAWYYLQPRSMTHSSRSLNATPRSCATPPGTRLSVDSTIGSLRLNLNYTADHVFPLATYDDLMNLLLESVDQRPITVSAVSILGELVSGKTEVAQPLVRLFTHTERIAPIIKALADHEISHLTDPTTIFRGNTLVSKMMDEAMRLSGLHYLHQTLRPVLSQIVAEKKPCEIDPSKIKDRSAVDTNLHNLQDYVERVFEAITKSADRCPKVLCQIFHDLRECAGEHFPSNREVRYSVVSGFIFLRFFAPAILGPKLFDLTTERLDAQTSRTLTLISKTIQSLGNLVSSRSSQQTCKEEFTVELYKKFCTEQHVDAVKHFLEVISTPSHASSSVHPAAAAATPLEPVLLKEGLMTKYPTSRKRFGRQFKQRHFRLTTHSLSYAKSKGKQPICDIPLQEIASVEQLKDKSFKMQNCFKIVHNDRSLIVQTTNCVEEREWFDLLHKICLMNSIRMQYFHPSAFVSGFYSCCGRSDENSPGCKKVLDKTMDYFQMDLVTALDPALDLQRIHTLIMSNMSVLESLLDPLTYHQSLSQTQHQQHNPLVPLATDLQKHSPQAFAEFKRTIEKLREKAYAIDRDHRDYKQGITRQLKYGSRQAPIGDDNYWHMMRAAGQLNQQHHQQQQHQQQQQQQQQQQLQQFQPQPVLPQMQNVRAYPYQPATSNMNAYCLHNMQYQQQRLPFHQQQQQHHQQLQQQQSQFQPLRSHQLQRHNNNLNNNNCGNGSSSSPSSTTSSVVAAPPSTTSSSQPAPPIY*RSRLCVFALPRAIITTR

Target ORF information:

RefSeq Version	NM_001274735.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ras GTPase activating protein 1 (RasGAP1), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274735.1

ORF Insert Sequence:

ATGCTGCTGA AGAAGAAGCG CTATATGTTC GACGAGCGAG ACGACAATAA TATTAACTCG 
CCCGTCGCCG TCGAGCCAAG CAGCAACAAC AACAAAATGG CCGATACACG CGAAGTGCGC 
ATTGAAGAGC AATTAAAAGT GAAAATTGGC GAGGCAAAGA ATCTGAGCAG TCGCAATGCG 
GCCAACACTA GCTGCAGCAC ACAGGGCACT CGCGATGTCT ACTGCACAAT TGCGTTGGAC 
CAGGAGGAGA TCTGCCGCAC ACCCACCATC GAACGCACAC TGACGCCGTT CTTCGGCGAG 
GAGCACCAGT TCAAGATTCC GCGCCGCTTC CGCTACCTGA CCATCTATCT GTGGGATCGT 
GATATGAAGC AGGATAAACC CATTGGCAAG ATAGCGATCA AGCGGGAGGA GCTGCACATG 
TACAATCACA AGGATCACTG GTTCTCATTG AGGCCAGTCG ACCAAGACTC CGAGGTTCAG 
GGCATGGTTA ATGTGGAGGT GGCATTTACG GAAGCCCAAC AGACCCAGTC GCTCTCCGAG 
GGCATAGATC TGGGACAGCA CACATTGCGG CATCATCAGA ACCTGCCACA CCACAGCCAC 
CAGCAGCGGG CGCATCTAAA CGATTACAAG GAGAACAGTG AACTGAGTAA CATACAGCGG 
GCATCAGCAG CTGCAGCGAG TTCCTCATCG GCAGCCATGA CACTCAAGAC ACGAGCGGCC 
GGTCTATTTG GTCACGTGCA TCATCCGCCA TCGCAGACTC AGCACTTTCC GATCATCAAT 
ACCACATCCA CGTCGTCGGA TCAGTTGTCC AATTGGAAAT CGCATGGGAG ATTCGTTGGT 
GTGACCATCA AAGTACCAGC CTGCGTGGAC CTGGCCAAGA AGCAGGGCAC GTGCGATCCA 
TTTGTGGTCT GTACCGCCCA CTATAGCAAC AAGCACCAGG TGACGCGACG AACTAAGCAG 
CGCAAAAAGA CCGTGGATCC GGAGTTTGAC GAGGCCATGT ACTTTGATCT TCACATCGAT 
GCGGATGCGG GTAGCACAAA TACCACTGGA AGTAACAAGA GTGCCGGTTC CCTGGAATCA 
TCAGCGAACA AGGGCTATTC TATATACCCG GTGGGCGGTG CGGATCTGGT GGAGATTGTG 
GTGAGCGTGT GGCACGACGC ACATGGAGCC ATGTCCGATA AAGTGTTCCT TGGTGAAGTG 
CGGCTACCAA TGCTCAATAA GCAGGAGCAG CAGGCAGTTA ATCCATCGGC TTGGTACTAT 
CTACAGCCCC GCTCCATGAC GCACAGTTCC CGCTCGCTGA ACGCCACGCC CCGCTCATGT 
GCGACGCCGC CGGGAACGAG ATTAAGCGTG GACTCGACCA TTGGATCACT GCGCTTAAAT 
CTAAACTACA CCGCCGATCA TGTCTTCCCG CTGGCCACAT ACGACGATCT TATGAATCTG 
CTCCTGGAAT CGGTTGATCA GCGACCCATC ACCGTGTCGG CTGTGTCGAT CCTAGGCGAG 
CTGGTCTCGG GCAAGACGGA GGTGGCCCAG CCGCTGGTGC GACTCTTTAC GCACACGGAG 
CGCATTGCGC CGATCATCAA GGCGCTGGCT GACCACGAGA TCTCCCATCT GACAGATCCC 
ACCACTATCT TCCGGGGTAA CACCTTGGTC TCTAAGATGA TGGATGAGGC AATGCGATTG 
TCTGGACTGC ACTACCTACA TCAGACATTG CGTCCGGTTT TGTCGCAGAT CGTTGCCGAA 
AAGAAACCAT GTGAGATCGA TCCCAGCAAG ATTAAAGATC GCTCTGCGGT GGACACGAAT 
CTGCACAATC TGCAAGATTA TGTGGAGCGG GTTTTTGAGG CAATCACCAA GAGCGCTGAT 
CGCTGTCCGA AAGTTCTGTG TCAGATCTTC CACGACCTTC GCGAGTGTGC GGGTGAGCAT 
TTCCCCAGCA ATCGAGAAGT GCGCTACTCC GTGGTGTCCG GCTTTATCTT CCTGCGCTTC 
TTTGCACCCG CTATCCTGGG ACCAAAGCTC TTCGATCTGA CCACCGAGCG ACTGGATGCC 
CAAACGAGTC GCACGCTGAC TCTCATCTCC AAGACGATCC AATCGCTGGG TAATCTGGTC 
AGCTCCCGGT CGTCGCAACA GACTTGCAAG GAGGAGTTCA CCGTCGAGCT GTACAAGAAG 
TTCTGCACGG AACAGCACGT GGATGCGGTT AAGCACTTTC TGGAGGTGAT CTCGACGCCC 
AGTCATGCGA GCAGCAGCGT TCATCCGGCG GCGGCGGCGG CAACGCCACT GGAACCAGTT 
TTACTTAAAG AGGGCCTGAT GACCAAGTAT CCGACATCCC GGAAGCGTTT TGGCCGGCAG 
TTCAAACAAC GCCACTTCCG CCTCACCACC CACTCGCTCA GCTATGCGAA GTCGAAGGGC 
AAACAACCCA TCTGTGATAT ACCGCTCCAG GAGATCGCCA GCGTGGAGCA GCTGAAGGAC 
AAGAGTTTCA AGATGCAGAA CTGCTTTAAG ATTGTGCACA ACGATCGCTC GCTGATTGTG 
CAGACAACGA ACTGCGTGGA GGAGCGCGAG TGGTTCGACC TGCTGCACAA GATATGTCTG 
ATGAACTCCA TCCGGATGCA GTACTTCCAT CCCTCGGCCT TTGTAAGTGG ATTCTACAGC 
TGCTGCGGCC GGTCGGATGA GAATTCACCG GGCTGTAAGA AGGTGTTGGA CAAGACGATG 
GACTACTTCC AGATGGATTT AGTAACGGCG CTGGATCCAG CGCTCGATCT GCAGCGCATC 
CACACGCTGA TCATGTCTAA CATGAGTGTG CTGGAGTCAC TGCTAGATCC GCTCACCTAT 
CACCAGTCTC TGTCGCAGAC GCAACACCAA CAGCACAATC CTCTGGTGCC GCTGGCCACC 
GATCTGCAGA AGCATTCACC ACAGGCCTTC GCTGAGTTCA AGCGAACGAT TGAGAAGCTG 
AGAGAGAAGG CCTATGCCAT CGATAGAGAT CATCGGGACT ACAAGCAGGG CATTACGCGG 
CAACTGAAAT ACGGCAGCAG ACAGGCACCC ATTGGCGATG ATAACTATTG GCATATGATG 
CGAGCCGCCG GCCAGTTGAA TCAGCAGCAT CACCAGCAGC AGCAACATCA GCAGCAACAG 
CAACAGCAGC AGCAGCAGCA ACTTCAGCAG TTCCAGCCGC AGCCAGTGCT GCCGCAAATG 
CAGAACGTTC GGGCGTATCC TTATCAGCCA GCTACGAGCA ACATGAACGC CTACTGTCTG 
CACAACATGC AGTATCAGCA GCAGAGATTG CCGTTCCATC AGCAGCAACA GCAGCACCAT 
CAGCAGCTCC AGCAACAGCA GTCGCAGTTC CAGCCCCTGC GCAGCCATCA ACTGCAGCGC 
CACAACAACA ATTTGAACAA CAATAACTGC GGCAATGGAT CCTCCTCCAG TCCATCATCG 
ACCACATCAA GTGTGGTGGC CGCACCGCCC AGCACCACAT CGTCATCGCA GCCAGCTCCA 
CCGATTTATT AGCGTTCAAG GCTGTGCGTA TTCGCTTTGC CGCGGGCCAT TATCACGACC 
AGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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RasGAP1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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