TrpA1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	TrpA1
Entrez Gene ID	39015
Full Name	Transient receptor potential cation channel A1
Synonyms	ANKTM1,Anktm1,CG5751,CG5761,CT18073,DmTRPA1,Dmel\CG5751,TRPA,TRPA1,Trp1A,TrpA,Trpa1,dANKTM1,dTRPA,dTRPA1,dTrpA,dTrpA1,dtrpA1,trpA1
General protein information	Preferred Names Transient receptor potential cation channel A1 Names CG5751 gene product from transcript CG5751-RK CG5751-PG CG5751-PH CG5751-PI CG5751-PJ CG5751-PK TrpA1-PG TrpA1-PH TrpA1-PI TrpA1-PJ TrpA1-PK dTRPA1 transient receptor potential A 1 transient receptor potential A1 transient receptor potential cation channel transient receptor potential cation channel A1 transient receptor potential cation channel A1 ortholog transient-receptor-potential A1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 66E3-66E3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001274673.1	NP_001261602.1	transient receptor potential cation channel A1, isoform K
NM_001104084.4	NP_001097554.4	transient receptor potential cation channel A1, isoform J
NM_001274671.1	NP_001261600.1	transient receptor potential cation channel A1, isoform G
NM_140006.5	NP_648263.5	transient receptor potential cation channel A1, isoform I
NM_001274672.1	NP_001261601.1	transient receptor potential cation channel A1, isoform H

Pathways from BioSystems

Homologies

Bos taurus (cattle)	TRPA1	XP_002692772.2
Canis lupus familiaris (dog)	TRPA1	XP_544123.2
Caenorhabditis elegans (roundworm)	trpa-1	NP_502249.3
Pan troglodytes (chimpanzee)	TRPA1	XP_519806.2
Mus musculus (house mouse)	Trpa1	NP_808449.1
Danio rerio (zebrafish)	trpa1a	NP_001007066.1
Danio rerio (zebrafish)	trpa1b	NP_001007067.1
Drosophila melanogaster (fruit fly)	TrpA1	NP_648263.5
Homo sapiens (human)	TRPA1	NP_015628.2
	TRPA1	XP_001083172.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004863	XP_314325.4
Rattus norvegicus (Norway rat)	Trpa1	NP_997491.1
Gallus gallus (chicken)	TRPA1	XP_418294.4
Xenopus tropicalis (tropical clawed frog)	trpa1	NP_001121434.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

H2O2-sensitive dTRPA1 isoforms promote avoidance of UV when adult Drosophila females are selecting sites for egg-laying.
Title: H2O2-Sensitive Isoforms of Drosophila melanogaster TRPA1 Act in Bitter-Sensing Gustatory Neurons to Promote Avoidance of UV During Egg-Laying.

Rh5 and Rh6 were required in trpA1-expressing neurons in the brain, ventral nerve cord, and body wall. Because Rh1 contributes to thermal selection in the comfortable range during the early to mid-third-instar stage, fine thermal discrimination depends on multiple rhodopsins.
Title: A Switch in Thermal Preference in Drosophila Larvae Depends on Multiple Rhodopsins.

Collectively, TRPA1 A isoform rapidly responds to natural sunlight intensities through its nucleophile sensitivity as a receptor of photochemically generated radicals, leading to an acute light-induced behavioral shift in Drosophila.
Title: Nucleophile sensitivity of

These findings demonstrate that Drosophila detects bacterial endotoxins via a gustatory pathway through TRPA1 activation as conserved molecular mechanism.
Title: Gustatory-mediated avoidance of bacterial lipopolysaccharides via TRPA1 activation in Drosophila.

Contribution of Drosophila TRPA1 to Metabolism.
Title: Contribution of Drosophila TRPA1 to Metabolism.

The following TrpA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TrpA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa17948	NM_001104084.4 Latest version!	Drosophila melanogaster transient receptor potential cation channel A1, transcript variant J (TrpA1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa17949	NM_140006.5 Latest version!	Drosophila melanogaster transient receptor potential cation channel A1, transcript variant I (TrpA1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa20763	NM_001274673.1 Latest version!	Drosophila melanogaster transient receptor potential cation channel A1, transcript variant K (TrpA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa20764	NM_001274672.1 Latest version!	Drosophila melanogaster transient receptor potential cation channel A1, transcript variant H (TrpA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa20765	NM_001274671.1 Latest version!	Drosophila melanogaster transient receptor potential cation channel A1, transcript variant G (TrpA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TrpA1 ( NM_001104084.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097554.4 Drosophila melanogaster transient receptor potential cation channel A1, transcript variant J (TrpA1), partial mRNA.

Price & Availability↑

CloneID	OFa17948
Clone ID Related Accession (Same CDS sequence)	NM_001104084.4
Accession Version	NM_001104084.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	transient receptor potential cation channel A1, isoform J
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001104084.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on the 5' end.

ATGACTTCGG GCGACAAGGA GACTCCTAAG AGGGAAGATT TCGCCAGTGC GCTGCGATTC 
TTGATGGGCG GCTGTGCCCG CGAACCGGAA ATGACTGCAA TGGCGCCGCT TAACCTGCCC 
AAAAAGTGGG CACGCATCCT GCGGATGTCC TCGACTCCAA AGATACCAAT CGTGGACTAT 
CTGGAGGCGG CTGAGTCCGG AAACCTTGAC GACTTCAAGC GACTCTTCAT GGCGGACAAC 
TCGCGCATTG CTTTAAAGGA TGCGAAAGGA CGAACGGCTG CCCATCAGGC GGCGGCCCGT 
AATAGGGTTA ACATTTTGCG GTACATTCGC GACCAGAATG GCGACTTTAA TGCGAAGGAT 
AATGCCGGCA ATACCCCGCT CCACATCGCC GTGGAGAGCG ATGCCTACGA CGCTCTGGAC 
TATCTATTGT CCATCCCAGT GGATACGGGA GTGCTGAACG AGAAGAAGCA GGCACCAGTG 
CACTTGGCCA CCGAGCTGAA CAAAGTGAAG TCCCTTCGGG TGATGGGTCA GTACCGCAAT 
GTCATCGATA TTCAGCAGGG CGGCGAACAT GGACGTACCG CTCTGCACTT GGCCGCCATC 
TATGATCACG AGGAGTGCGC TCGCATCCTG ATAACTGAGT TCGATGCATG CCCACGTAAG 
CCCTGTAACA ATGGTTATTA TCCCATACAC GAAGCGGCCA AGAATGCCAG CTCCAAGACA 
ATGGAGGTCT TCTTCCAGTG GGGCGAGCAG CGCGGCTGCA CCCGCGAGGA GATGATATCC 
TTCTACGACT CGGAGGGCAA TGTGCCGCTC CATTCGGCTG TCCATGGTGG CGACATCAAG 
GCTGTGGAGC TGTGCCTCAA GTCCGGGGCC AAGATATCTA CGCAGCAACA CGATCTCTCG 
ACGCCAGTGC ACCTGGCTTG TGCCCAGGGA GCCATAGACA TTGTAAAGCT CATGTTCGAG 
ATGCAGCCAA TGGAGAAGCG ACTATGTCTG AGTTGCACGG ATGTGCAGAA GATGACGCCG 
CTGCACTGCG CCTCCATGTT CGATCATCCG GACATTGTGT CCTATCTGGT GGCCGAGGGA 
GCGGACATCA ATGCCCTGGA CAAGGAGCAT CGCTCTCCGT TACTGCTGGC GGCATCTCGT 
AGCGGTTGGA AAACAGTCCA CCTCCTGATT CGCCTGGGGG CGTGCATTAG TGTCAAGGAC 
GCCGCCGCCC GCAATGTGCT GCACTTCGTC ATCATGAACG GCGGCCGGCT GACGGACTTC 
GCGGAGCAGG TGGCCAATTG CCAGACGCAG GCGCAGCTGA AGCTGCTGCT CAACGAGAAG 
GACAGCATGG GCTGCTCGCC GCTGCACTAC GCCAGTCGGG ATGGACACAT CCGTTCGTTG 
GAGAACCTCA TTCGACTGGG AGCCTGCATC AACCTGAAGA ACAACAACAA CGAGAGTCCG 
CTGCACTTTG CCGCACGCTA CGGAAGATAC AATACGGTGC GGCAGCTCTT GGATTCCGAG 
AAGGGATCCT TCATCATCAA CGAAAGTGAC GGTGCAGGGA TGACACCACT GCACATATCC 
TCGCAGCAAG GACACACGCG AGTGGTGCAG CTGCTACTCA ATCGAGGAGC CCTGCTCCAT 
CGGGACCACA CCGGACGCAA TCCTCTCCAG CTAGCGGCCA TGTCCGGATA CACCGAGACC 
ATCGAGCTGC TGCACTCGGT GCACTCGCAT CTGCTCGATC AGGTGGACAA GGATGGGAAC 
ACCGCTCTTC ACCTGGCCAC CATGGAGAAT AAGCCCCATG CGATCTCCGT GCTGATGTCT 
ATGGGCTGTA AGCTGGTCTA TAACGTTCTG GACATGAGTG CCATTGACTA TGCCATCTAC 
TACAAATATC CGGAGGCTGC CCTGGCCATG GTCACCCACG AGGAGCGGGC CAACGAGGTG 
ATGGCTCTGC GTTCCGACAA GCATCCGTGC GTGACCCTCG CCTTAATTGC CTCCATGCCC 
AAGGTATTCG AGGCGGTGCA GGACAAGTGC ATTACCAAGG CCAATTGCAA GAAGGACTCG 
AAGAGTTTCT ACATAAAATA TTCGTTTTGG CCCTACCAAA AGACACCCGA ACAGATTGAG 
GCCAAGCGCA AAGAGTTCAA TGACCCCAAG TGGCGACCCG CGCCTTTGGC CGTGGTGAAC 
ACCATGGTAA CACATGGCAG GGTGGAGCTG CTGGCCCATC CGCTCAGTCA GAAGTATCTG 
CAGATGAAGT GGAACTCCTA CGGCAAGTAC TTTCACCTGG CCAACCTGCT AATCTACTCG 
ATATTCCTGG TCTTTGTAAC CATCTACTCT TCGCTGATGA TGAACAACAT CGAACTGAAG 
GCTGGGGACA ACAAGACGAT GAGTCAATAC TGCAATATGG GATGGGAGCA GCTGACCATG 
AATCTCTCGC AGAACCCGTC GGTGGCATCA CAGATTCGTT TGGATTCCTG CGAGGAGCGT 
ATAAATAGAA CCACTGCAAT ACTTTTCTGT GCGGTGGTCA TCGTGGTCTA TATACTGCTC 
AACTCGATGC GGGAACTAAT ACAGATATAC CAGCAGAAAT TGCACTATAT CCTGGAGACA 
GTTAATTTGA TATCCTGGGT GCTGTACATC TCGGCTTTGG TGATGGTAAC ACCGGCATTT 
CAGCCGGATG GAGGAATCAA TACCATTCAT TACTCGGCCG CTTCAATAGC AGTCTTTCTG 
TCGTGGTTCC GATTGCTACT GTTCCTGCAA AGATTCGACC AGGTCGGCAT CTATGTGGTC 
ATGTTCTTGG AGATTCTGCA GACGCTCATT AAAGTGCTGA TGGTATTCTC CATACTTATA 
ATCGCCTTTG GTCTGGCTTT CTATATACTA CTTTCAAAGA TTATTGACCC CCAACCGAAC 
CACTTGTCCT TCTCCAACAT ACCCATGTCC TTGCTGCGAA CTTTCTCAAT GATGCTGGGC 
GAGCTGGACT TTGTGGGTAC CTATGTGAAC ACCTACTATC GGGATCAGTT GAAAGTGCCC 
ATGACATCCT TTTTGATTTT GAGTGTCTTT ATGATCCTTA TGCCCATTCT TCTGATGAAC 
TTGCTCATCG GTTTGGCCGT CGGCGATATT GAGTCAGTGC GTCGCAATGC CCAGCTCAAG 
AGACTGGCCA TGCAGGTGGT GCTCCACACG GAGCTGGAGA GGAAGTTGCC CCATGTCTGG 
CTGCAGCGAG TTGACAAGAT GGAGCTGATT GAGTATCCCA ATGAAACCAA GTGCAAGCTG 
GGCTTCTGCG ATTTCATCCT GCGCAAGTGG TTCTCGAATC CATTCACCGA GGATTCCTCC 
ATGGACGTCA TCTCCTTCGA CAACAATGAT GACTACATCA ACGCAGAATT GGAACGGCAG 
AGGCGAAAGT TGCGCGACAT AAGTCGCATG CTGGAGCAAC AGCACCATCT GGTTCGGCTC 
ATTGTCCAAA AGATGGAGAT CAAGACGGAG GCGGATGACG TGGACGAGGG TATATCCCCA 
AACGAGTTGC GATCCGTCGT CGGTTTGAGA TCGGCAGGCG GAAATCGATG GAACTCGCCG 
CGAGTCCGGA ATAAACTCCG AGCCGCCCTG AGCTTCAATA AGAGCATGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097554.4
CDS	1..3591
Translation	MTSGDKETPKREDFASALRFLMGGCAREPEMTAMAPLNLPKKWARILRMSSTPKIPIVDYLEAAESGNLDDFKRLFMADNSRIALKDAKGRTAAHQAAARNRVNILRYIRDQNGDFNAKDNAGNTPLHIAVESDAYDALDYLLSIPVDTGVLNEKKQAPVHLATELNKVKSLRVMGQYRNVIDIQQGGEHGRTALHLAAIYDHEECARILITEFDACPRKPCNNGYYPIHEAAKNASSKTMEVFFQWGEQRGCTREEMISFYDSEGNVPLHSAVHGGDIKAVELCLKSGAKISTQQHDLSTPVHLACAQGAIDIVKLMFEMQPMEKRLCLSCTDVQKMTPLHCASMFDHPDIVSYLVAEGADINALDKEHRSPLLLAASRSGWKTVHLLIRLGACISVKDAAARNVLHFVIMNGGRLTDFAEQVANCQTQAQLKLLLNEKDSMGCSPLHYASRDGHIRSLENLIRLGACINLKNNNNESPLHFAARYGRYNTVRQLLDSEKGSFIINESDGAGMTPLHISSQQGHTRVVQLLLNRGALLHRDHTGRNPLQLAAMSGYTETIELLHSVHSHLLDQVDKDGNTALHLATMENKPHAISVLMSMGCKLVYNVLDMSAIDYAIYYKYPEAALAMVTHEERANEVMALRSDKHPCVTLALIASMPKVFEAVQDKCITKANCKKDSKSFYIKYSFWPYQKTPEQIEAKRKEFNDPKWRPAPLAVVNTMVTHGRVELLAHPLSQKYLQMKWNSYGKYFHLANLLIYSIFLVFVTIYSSLMMNNIELKAGDNKTMSQYCNMGWEQLTMNLSQNPSVASQIRLDSCEERINRTTAILFCAVVIVVYILLNSMRELIQIYQQKLHYILETVNLISWVLYISALVMVTPAFQPDGGINTIHYSAASIAVFLSWFRLLLFLQRFDQVGIYVVMFLEILQTLIKVLMVFSILIIAFGLAFYILLSKIIDPQPNHLSFSNIPMSLLRTFSMMLGELDFVGTYVNTYYRDQLKVPMTSFLILSVFMILMPILLMNLLIGLAVGDIESVRRNAQLKRLAMQVVLHTELERKLPHVWLQRVDKMELIEYPNETKCKLGFCDFILRKWFSNPFTEDSSMDVISFDNNDDYINAELERQRRKLRDISRMLEQQHHLVRLIVQKMEIKTEADDVDEGISPNELRSVVGLRSAGGNRWNSPRVRNKLRAALSFNKSM

Target ORF information:

RefSeq Version	NM_001104084.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster transient receptor potential cation channel A1, transcript variant J (TrpA1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001104084.4

ORF Insert Sequence:

ATGACTTCGG GCGACAAGGA GACTCCTAAG AGGGAAGATT TCGCCAGTGC GCTGCGATTC 
TTGATGGGCG GCTGTGCCCG CGAACCGGAA ATGACTGCAA TGGCGCCGCT TAACCTGCCC 
AAAAAGTGGG CACGCATCCT GCGGATGTCC TCGACTCCAA AGATACCAAT CGTGGACTAT 
CTGGAGGCGG CTGAGTCCGG AAACCTTGAC GACTTCAAGC GACTCTTCAT GGCGGACAAC 
TCGCGCATTG CTTTAAAGGA TGCGAAAGGA CGAACGGCTG CCCATCAGGC GGCGGCCCGT 
AATAGGGTTA ACATTTTGCG GTACATTCGC GACCAGAATG GCGACTTTAA TGCGAAGGAT 
AATGCCGGCA ATACCCCGCT CCACATCGCC GTGGAGAGCG ATGCCTACGA CGCTCTGGAC 
TATCTATTGT CCATCCCAGT GGATACGGGA GTGCTGAACG AGAAGAAGCA GGCACCAGTG 
CACTTGGCCA CCGAGCTGAA CAAAGTGAAG TCCCTTCGGG TGATGGGTCA GTACCGCAAT 
GTCATCGATA TTCAGCAGGG CGGCGAACAT GGACGTACCG CTCTGCACTT GGCCGCCATC 
TATGATCACG AGGAGTGCGC TCGCATCCTG ATAACTGAGT TCGATGCATG CCCACGTAAG 
CCCTGTAACA ATGGTTATTA TCCCATACAC GAAGCGGCCA AGAATGCCAG CTCCAAGACA 
ATGGAGGTCT TCTTCCAGTG GGGCGAGCAG CGCGGCTGCA CCCGCGAGGA GATGATATCC 
TTCTACGACT CGGAGGGCAA TGTGCCGCTC CATTCGGCTG TCCATGGTGG CGACATCAAG 
GCTGTGGAGC TGTGCCTCAA GTCCGGGGCC AAGATATCTA CGCAGCAACA CGATCTCTCG 
ACGCCAGTGC ACCTGGCTTG TGCCCAGGGA GCCATAGACA TTGTAAAGCT CATGTTCGAG 
ATGCAGCCAA TGGAGAAGCG ACTATGTCTG AGTTGCACGG ATGTGCAGAA GATGACGCCG 
CTGCACTGCG CCTCCATGTT CGATCATCCG GACATTGTGT CCTATCTGGT GGCCGAGGGA 
GCGGACATCA ATGCCCTGGA CAAGGAGCAT CGCTCTCCGT TACTGCTGGC GGCATCTCGT 
AGCGGTTGGA AAACAGTCCA CCTCCTGATT CGCCTGGGGG CGTGCATTAG TGTCAAGGAC 
GCCGCCGCCC GCAATGTGCT GCACTTCGTC ATCATGAACG GCGGCCGGCT GACGGACTTC 
GCGGAGCAGG TGGCCAATTG CCAGACGCAG GCGCAGCTGA AGCTGCTGCT CAACGAGAAG 
GACAGCATGG GCTGCTCGCC GCTGCACTAC GCCAGTCGGG ATGGACACAT CCGTTCGTTG 
GAGAACCTCA TTCGACTGGG AGCCTGCATC AACCTGAAGA ACAACAACAA CGAGAGTCCG 
CTGCACTTTG CCGCACGCTA CGGAAGATAC AATACGGTGC GGCAGCTCTT GGATTCCGAG 
AAGGGATCCT TCATCATCAA CGAAAGTGAC GGTGCAGGGA TGACACCACT GCACATATCC 
TCGCAGCAAG GACACACGCG AGTGGTGCAG CTGCTACTCA ATCGAGGAGC CCTGCTCCAT 
CGGGACCACA CCGGACGCAA TCCTCTCCAG CTAGCGGCCA TGTCCGGATA CACCGAGACC 
ATCGAGCTGC TGCACTCGGT GCACTCGCAT CTGCTCGATC AGGTGGACAA GGATGGGAAC 
ACCGCTCTTC ACCTGGCCAC CATGGAGAAT AAGCCCCATG CGATCTCCGT GCTGATGTCT 
ATGGGCTGTA AGCTGGTCTA TAACGTTCTG GACATGAGTG CCATTGACTA TGCCATCTAC 
TACAAATATC CGGAGGCTGC CCTGGCCATG GTCACCCACG AGGAGCGGGC CAACGAGGTG 
ATGGCTCTGC GTTCCGACAA GCATCCGTGC GTGACCCTCG CCTTAATTGC CTCCATGCCC 
AAGGTATTCG AGGCGGTGCA GGACAAGTGC ATTACCAAGG CCAATTGCAA GAAGGACTCG 
AAGAGTTTCT ACATAAAATA TTCGTTTTGG CCCTACCAAA AGACACCCGA ACAGATTGAG 
GCCAAGCGCA AAGAGTTCAA TGACCCCAAG TGGCGACCCG CGCCTTTGGC CGTGGTGAAC 
ACCATGGTAA CACATGGCAG GGTGGAGCTG CTGGCCCATC CGCTCAGTCA GAAGTATCTG 
CAGATGAAGT GGAACTCCTA CGGCAAGTAC TTTCACCTGG CCAACCTGCT AATCTACTCG 
ATATTCCTGG TCTTTGTAAC CATCTACTCT TCGCTGATGA TGAACAACAT CGAACTGAAG 
GCTGGGGACA ACAAGACGAT GAGTCAATAC TGCAATATGG GATGGGAGCA GCTGACCATG 
AATCTCTCGC AGAACCCGTC GGTGGCATCA CAGATTCGTT TGGATTCCTG CGAGGAGCGT 
ATAAATAGAA CCACTGCAAT ACTTTTCTGT GCGGTGGTCA TCGTGGTCTA TATACTGCTC 
AACTCGATGC GGGAACTAAT ACAGATATAC CAGCAGAAAT TGCACTATAT CCTGGAGACA 
GTTAATTTGA TATCCTGGGT GCTGTACATC TCGGCTTTGG TGATGGTAAC ACCGGCATTT 
CAGCCGGATG GAGGAATCAA TACCATTCAT TACTCGGCCG CTTCAATAGC AGTCTTTCTG 
TCGTGGTTCC GATTGCTACT GTTCCTGCAA AGATTCGACC AGGTCGGCAT CTATGTGGTC 
ATGTTCTTGG AGATTCTGCA GACGCTCATT AAAGTGCTGA TGGTATTCTC CATACTTATA 
ATCGCCTTTG GTCTGGCTTT CTATATACTA CTTTCAAAGA TTATTGACCC CCAACCGAAC 
CACTTGTCCT TCTCCAACAT ACCCATGTCC TTGCTGCGAA CTTTCTCAAT GATGCTGGGC 
GAGCTGGACT TTGTGGGTAC CTATGTGAAC ACCTACTATC GGGATCAGTT GAAAGTGCCC 
ATGACATCCT TTTTGATTTT GAGTGTCTTT ATGATCCTTA TGCCCATTCT TCTGATGAAC 
TTGCTCATCG GTTTGGCCGT CGGCGATATT GAGTCAGTGC GTCGCAATGC CCAGCTCAAG 
AGACTGGCCA TGCAGGTGGT GCTCCACACG GAGCTGGAGA GGAAGTTGCC CCATGTCTGG 
CTGCAGCGAG TTGACAAGAT GGAGCTGATT GAGTATCCCA ATGAAACCAA GTGCAAGCTG 
GGCTTCTGCG ATTTCATCCT GCGCAAGTGG TTCTCGAATC CATTCACCGA GGATTCCTCC 
ATGGACGTCA TCTCCTTCGA CAACAATGAT GACTACATCA ACGCAGAATT GGAACGGCAG 
AGGCGAAAGT TGCGCGACAT AAGTCGCATG CTGGAGCAAC AGCACCATCT GGTTCGGCTC 
ATTGTCCAAA AGATGGAGAT CAAGACGGAG GCGGATGACG TGGACGAGGG TATATCCCCA 
AACGAGTTGC GATCCGTCGT CGGTTTGAGA TCGGCAGGCG GAAATCGATG GAACTCGCCG 
CGAGTCCGGA ATAAACTCCG AGCCGCCCTG AGCTTCAATA AGAGCATGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TrpA1 ( NM_140006.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648263.5 Drosophila melanogaster transient receptor potential cation channel A1, transcript variant I (TrpA1), partial mRNA.

Price & Availability↑

CloneID	OFa17949
Clone ID Related Accession (Same CDS sequence)	NM_140006.5
Accession Version	NM_140006.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	transient receptor potential cation channel A1, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140006.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on the 5' end.

ATGACTTCGG GCGACAAGGA GACTCCTAAG AGGGAAGATT TCGCCAGTGC GCTGCGATTC 
TTGATGGGCG GCTGTGCCCG CGAACCGGAA ATGACTGCAA TGGCGCCGCT TAACCTGCCC 
AAAAAGTGGG CACGCATCCT GCGGATGTCC TCGACTCCAA AGATACCAAT CGTGGACTAT 
CTGGAGGCGG CTGAGTCCGG AAACCTTGAC GACTTCAAGC GACTCTTCAT GGCGGACAAC 
TCGCGCATTG CTTTAAAGGA TGCGAAAGGA CGAACGGCTG CCCATCAGGC GGCGGCCCGT 
AATAGGGTTA ACATTTTGCG GTACATTCGC GACCAGAATG GCGACTTTAA TGCGAAGGAT 
AATGCCGGCA ATACCCCGCT CCACATCGCC GTGGAGAGCG ATGCCTACGA CGCTCTGGAC 
TATCTATTGT CCATCCCAGT GGATACGGGA GTGCTGAACG AGAAGAAGCA GGCACCAGTG 
CACTTGGCCA CCGAGCTGAA CAAAGTGAAG TCCCTTCGGG TGATGGGTCA GTACCGCAAT 
GTCATCGATA TTCAGCAGGG CGGCGAACAT GGACGTACCG CTCTGCACTT GGCCGCCATC 
TATGATCACG AGGAGTGCGC TCGCATCCTG ATAACTGAGT TCGATGCATG CCCACGTAAG 
CCCTGTAACA ATGGTTATTA TCCCATACAC GAAGCGGCCA AGAATGCCAG CTCCAAGACA 
ATGGAGGTCT TCTTCCAGTG GGGCGAGCAG CGCGGCTGCA CCCGCGAGGA GATGATATCC 
TTCTACGACT CGGAGGGCAA TGTGCCGCTC CATTCGGCTG TCCATGGTGG CGACATCAAG 
GCTGTGGAGC TGTGCCTCAA GTCCGGGGCC AAGATATCTA CGCAGCAACA CGATCTCTCG 
ACGCCAGTGC ACCTGGCTTG TGCCCAGGGA GCCATAGACA TTGTAAAGCT CATGTTCGAG 
ATGCAGCCAA TGGAGAAGCG ACTATGTCTG AGTTGCACGG ATGTGCAGAA GATGACGCCG 
CTGCACTGCG CCTCCATGTT CGATCATCCG GACATTGTGT CCTATCTGGT GGCCGAGGGA 
GCGGACATCA ATGCCCTGGA CAAGGAGCAT CGCTCTCCGT TACTGCTGGC GGCATCTCGT 
AGCGGTTGGA AAACAGTCCA CCTCCTGATT CGCCTGGGGG CGTGCATTAG TGTCAAGGAC 
GCCGCCGCCC GCAATGTGCT GCACTTCGTC ATCATGAACG GCGGCCGGCT GACGGACTTC 
GCGGAGCAGG TGGCCAATTG CCAGACGCAG GCGCAGCTGA AGCTGCTGCT CAACGAGAAG 
GACAGCATGG GCTGCTCGCC GCTGCACTAC GCCAGTCGGG ATGGACACAT CCGTTCGTTG 
GAGAACCTCA TTCGACTGGG AGCCTGCATC AACCTGAAGA ACAACAACAA CGAGAGTCCG 
CTGCACTTTG CCGCACGCTA CGGAAGATAC AATACGGTGC GGCAGCTCTT GGATTCCGAG 
AAGGGATCCT TCATCATCAA CGAAAGTGAC GGTGCAGGGA TGACACCACT GCACATATCC 
TCGCAGCAAG GACACACGCG AGTGGTGCAG CTGCTACTCA ATCGAGGAGC CCTGCTCCAT 
CGGGACCACA CCGGACGCAA TCCTCTCCAG CTAGCGGCCA TGTCCGGATA CACCGAGACC 
ATCGAGCTGC TGCACTCGGT GCACTCGCAT CTGCTCGATC AGGTGGACAA GGATGGGAAC 
ACCGCTCTTC ACCTGGCCAC CATGGAGAAT AAGCCCCATG CGATCTCCGT GCTGATGTCT 
ATGGGCTGTA AGCTGGTCTA TAACGTTCTG GACATGAGTG CCATTGACTA TGCCATCTAC 
TACAAATATC CGGAGGCTGC CCTGGCCATG GTCACCCACG AGGAGCGGGC CAACGAGGTG 
ATGGCTCTGC GTTCCGACAA GCATCCGTGC GTGACCCTCG CCTTAATTGC CTCCATGCCC 
AAGGTATTCG AGGCGGTGCA GGACAAGTGC ATTACCAAGG CCAATTGCAA GAAGGACTCG 
AAGAGTTTCT ACATCAAATA CTCTTTCGCA TTCTTGCAAT GCCCCTTTAT GTTTGCCAAG 
ATTGATGAGA AAACCGGAGA GTCGATTACG ACCGCCAGTC CCATTCCGTT GCCGGCTTTG 
AATACCATGG TAACACATGG CAGGGTGGAG CTGCTGGCCC ATCCGCTCAG TCAGAAGTAT 
CTGCAGATGA AGTGGAACTC CTACGGCAAG TACTTTCACC TGGCCAACCT GCTAATCTAC 
TCGATATTCC TGGTCTTTGT AACCATCTAC TCTTCGCTGA TGATGAACAA CATCGAACTG 
AAGGCTGGGG ACAACAAGAC GATGAGTCAA TACTGCAATA TGGGATGGGA GCAGCTGACC 
ATGAATCTCT CGCAGAACCC GTCGGTGGCA TCACAGATTC GTTTGGATTC CTGCGAGGAG 
CGTATAAATA GAACCACTGC AATACTTTTC TGTGCGGTGG TCATCGTGGT CTATATACTG 
CTCAACTCGA TGCGGGAACT AATACAGATA TACCAGCAGA AATTGCACTA TATCCTGGAG 
ACAGTTAATT TGATATCCTG GGTGCTGTAC ATCTCGGCTT TGGTGATGGT AACACCGGCA 
TTTCAGCCGG ATGGAGGAAT CAATACCATT CATTACTCGG CCGCTTCAAT AGCAGTCTTT 
CTGTCGTGGT TCCGATTGCT ACTGTTCCTG CAAAGATTCG ACCAGGTCGG CATCTATGTG 
GTCATGTTCT TGGAGATTCT GCAGACGCTC ATTAAAGTGC TGATGGTATT CTCCATACTT 
ATAATCGCCT TTGGTCTGGC TTTCTATATA CTACTTTCAA AGATTATTGA CCCCCAACCG 
AACCACTTGT CCTTCTCCAA CATACCCATG TCCTTGCTGC GAACTTTCTC AATGATGCTG 
GGCGAGCTGG ACTTTGTGGG TACCTATGTG AACACCTACT ATCGGGATCA GTTGAAAGTG 
CCCATGACAT CCTTTTTGAT TTTGAGTGTC TTTATGATCC TTATGCCCAT TCTTCTGATG 
AACTTGCTCA TCGGTTTGGC CGTCGGCGAT ATTGAGTCAG TGCGTCGCAA TGCCCAGCTC 
AAGAGACTGG CCATGCAGGT GGTGCTCCAC ACGGAGCTGG AGAGGAAGTT GCCCCATGTC 
TGGCTGCAGC GAGTTGACAA GATGGAGCTG ATTGAGTATC CCAATGAAAC CAAGTGCAAG 
CTGGGCTTCT GCGATTTCAT CCTGCGCAAG TGGTTCTCGA ATCCATTCAC CGAGGATTCC 
TCCATGGACG TCATCTCCTT CGACAACAAT GATGACTACA TCAACGCAGA ATTGGAACGG 
CAGAGGCGAA AGTTGCGCGA CATAAGTCGC ATGCTGGAGC AACAGCACCA TCTGGTTCGG 
CTCATTGTCC AAAAGATGGA GATCAAGACG GAGGCGGATG ACGTGGACGA GGGTATATCC 
CCAAACGAGT TGCGATCCGT CGTCGGTTTG AGATCGGCAG GCGGAAATCG ATGGAACTCG 
CCGCGAGTCC GGAATAAACT CCGAGCCGCC CTGAGCTTCA ATAAGAGCAT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648263.5
CDS	1..3594
Translation	MTSGDKETPKREDFASALRFLMGGCAREPEMTAMAPLNLPKKWARILRMSSTPKIPIVDYLEAAESGNLDDFKRLFMADNSRIALKDAKGRTAAHQAAARNRVNILRYIRDQNGDFNAKDNAGNTPLHIAVESDAYDALDYLLSIPVDTGVLNEKKQAPVHLATELNKVKSLRVMGQYRNVIDIQQGGEHGRTALHLAAIYDHEECARILITEFDACPRKPCNNGYYPIHEAAKNASSKTMEVFFQWGEQRGCTREEMISFYDSEGNVPLHSAVHGGDIKAVELCLKSGAKISTQQHDLSTPVHLACAQGAIDIVKLMFEMQPMEKRLCLSCTDVQKMTPLHCASMFDHPDIVSYLVAEGADINALDKEHRSPLLLAASRSGWKTVHLLIRLGACISVKDAAARNVLHFVIMNGGRLTDFAEQVANCQTQAQLKLLLNEKDSMGCSPLHYASRDGHIRSLENLIRLGACINLKNNNNESPLHFAARYGRYNTVRQLLDSEKGSFIINESDGAGMTPLHISSQQGHTRVVQLLLNRGALLHRDHTGRNPLQLAAMSGYTETIELLHSVHSHLLDQVDKDGNTALHLATMENKPHAISVLMSMGCKLVYNVLDMSAIDYAIYYKYPEAALAMVTHEERANEVMALRSDKHPCVTLALIASMPKVFEAVQDKCITKANCKKDSKSFYIKYSFAFLQCPFMFAKIDEKTGESITTASPIPLPALNTMVTHGRVELLAHPLSQKYLQMKWNSYGKYFHLANLLIYSIFLVFVTIYSSLMMNNIELKAGDNKTMSQYCNMGWEQLTMNLSQNPSVASQIRLDSCEERINRTTAILFCAVVIVVYILLNSMRELIQIYQQKLHYILETVNLISWVLYISALVMVTPAFQPDGGINTIHYSAASIAVFLSWFRLLLFLQRFDQVGIYVVMFLEILQTLIKVLMVFSILIIAFGLAFYILLSKIIDPQPNHLSFSNIPMSLLRTFSMMLGELDFVGTYVNTYYRDQLKVPMTSFLILSVFMILMPILLMNLLIGLAVGDIESVRRNAQLKRLAMQVVLHTELERKLPHVWLQRVDKMELIEYPNETKCKLGFCDFILRKWFSNPFTEDSSMDVISFDNNDDYINAELERQRRKLRDISRMLEQQHHLVRLIVQKMEIKTEADDVDEGISPNELRSVVGLRSAGGNRWNSPRVRNKLRAALSFNKSM

Target ORF information:

RefSeq Version	NM_140006.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster transient receptor potential cation channel A1, transcript variant I (TrpA1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140006.5

ORF Insert Sequence:

ATGACTTCGG GCGACAAGGA GACTCCTAAG AGGGAAGATT TCGCCAGTGC GCTGCGATTC 
TTGATGGGCG GCTGTGCCCG CGAACCGGAA ATGACTGCAA TGGCGCCGCT TAACCTGCCC 
AAAAAGTGGG CACGCATCCT GCGGATGTCC TCGACTCCAA AGATACCAAT CGTGGACTAT 
CTGGAGGCGG CTGAGTCCGG AAACCTTGAC GACTTCAAGC GACTCTTCAT GGCGGACAAC 
TCGCGCATTG CTTTAAAGGA TGCGAAAGGA CGAACGGCTG CCCATCAGGC GGCGGCCCGT 
AATAGGGTTA ACATTTTGCG GTACATTCGC GACCAGAATG GCGACTTTAA TGCGAAGGAT 
AATGCCGGCA ATACCCCGCT CCACATCGCC GTGGAGAGCG ATGCCTACGA CGCTCTGGAC 
TATCTATTGT CCATCCCAGT GGATACGGGA GTGCTGAACG AGAAGAAGCA GGCACCAGTG 
CACTTGGCCA CCGAGCTGAA CAAAGTGAAG TCCCTTCGGG TGATGGGTCA GTACCGCAAT 
GTCATCGATA TTCAGCAGGG CGGCGAACAT GGACGTACCG CTCTGCACTT GGCCGCCATC 
TATGATCACG AGGAGTGCGC TCGCATCCTG ATAACTGAGT TCGATGCATG CCCACGTAAG 
CCCTGTAACA ATGGTTATTA TCCCATACAC GAAGCGGCCA AGAATGCCAG CTCCAAGACA 
ATGGAGGTCT TCTTCCAGTG GGGCGAGCAG CGCGGCTGCA CCCGCGAGGA GATGATATCC 
TTCTACGACT CGGAGGGCAA TGTGCCGCTC CATTCGGCTG TCCATGGTGG CGACATCAAG 
GCTGTGGAGC TGTGCCTCAA GTCCGGGGCC AAGATATCTA CGCAGCAACA CGATCTCTCG 
ACGCCAGTGC ACCTGGCTTG TGCCCAGGGA GCCATAGACA TTGTAAAGCT CATGTTCGAG 
ATGCAGCCAA TGGAGAAGCG ACTATGTCTG AGTTGCACGG ATGTGCAGAA GATGACGCCG 
CTGCACTGCG CCTCCATGTT CGATCATCCG GACATTGTGT CCTATCTGGT GGCCGAGGGA 
GCGGACATCA ATGCCCTGGA CAAGGAGCAT CGCTCTCCGT TACTGCTGGC GGCATCTCGT 
AGCGGTTGGA AAACAGTCCA CCTCCTGATT CGCCTGGGGG CGTGCATTAG TGTCAAGGAC 
GCCGCCGCCC GCAATGTGCT GCACTTCGTC ATCATGAACG GCGGCCGGCT GACGGACTTC 
GCGGAGCAGG TGGCCAATTG CCAGACGCAG GCGCAGCTGA AGCTGCTGCT CAACGAGAAG 
GACAGCATGG GCTGCTCGCC GCTGCACTAC GCCAGTCGGG ATGGACACAT CCGTTCGTTG 
GAGAACCTCA TTCGACTGGG AGCCTGCATC AACCTGAAGA ACAACAACAA CGAGAGTCCG 
CTGCACTTTG CCGCACGCTA CGGAAGATAC AATACGGTGC GGCAGCTCTT GGATTCCGAG 
AAGGGATCCT TCATCATCAA CGAAAGTGAC GGTGCAGGGA TGACACCACT GCACATATCC 
TCGCAGCAAG GACACACGCG AGTGGTGCAG CTGCTACTCA ATCGAGGAGC CCTGCTCCAT 
CGGGACCACA CCGGACGCAA TCCTCTCCAG CTAGCGGCCA TGTCCGGATA CACCGAGACC 
ATCGAGCTGC TGCACTCGGT GCACTCGCAT CTGCTCGATC AGGTGGACAA GGATGGGAAC 
ACCGCTCTTC ACCTGGCCAC CATGGAGAAT AAGCCCCATG CGATCTCCGT GCTGATGTCT 
ATGGGCTGTA AGCTGGTCTA TAACGTTCTG GACATGAGTG CCATTGACTA TGCCATCTAC 
TACAAATATC CGGAGGCTGC CCTGGCCATG GTCACCCACG AGGAGCGGGC CAACGAGGTG 
ATGGCTCTGC GTTCCGACAA GCATCCGTGC GTGACCCTCG CCTTAATTGC CTCCATGCCC 
AAGGTATTCG AGGCGGTGCA GGACAAGTGC ATTACCAAGG CCAATTGCAA GAAGGACTCG 
AAGAGTTTCT ACATCAAATA CTCTTTCGCA TTCTTGCAAT GCCCCTTTAT GTTTGCCAAG 
ATTGATGAGA AAACCGGAGA GTCGATTACG ACCGCCAGTC CCATTCCGTT GCCGGCTTTG 
AATACCATGG TAACACATGG CAGGGTGGAG CTGCTGGCCC ATCCGCTCAG TCAGAAGTAT 
CTGCAGATGA AGTGGAACTC CTACGGCAAG TACTTTCACC TGGCCAACCT GCTAATCTAC 
TCGATATTCC TGGTCTTTGT AACCATCTAC TCTTCGCTGA TGATGAACAA CATCGAACTG 
AAGGCTGGGG ACAACAAGAC GATGAGTCAA TACTGCAATA TGGGATGGGA GCAGCTGACC 
ATGAATCTCT CGCAGAACCC GTCGGTGGCA TCACAGATTC GTTTGGATTC CTGCGAGGAG 
CGTATAAATA GAACCACTGC AATACTTTTC TGTGCGGTGG TCATCGTGGT CTATATACTG 
CTCAACTCGA TGCGGGAACT AATACAGATA TACCAGCAGA AATTGCACTA TATCCTGGAG 
ACAGTTAATT TGATATCCTG GGTGCTGTAC ATCTCGGCTT TGGTGATGGT AACACCGGCA 
TTTCAGCCGG ATGGAGGAAT CAATACCATT CATTACTCGG CCGCTTCAAT AGCAGTCTTT 
CTGTCGTGGT TCCGATTGCT ACTGTTCCTG CAAAGATTCG ACCAGGTCGG CATCTATGTG 
GTCATGTTCT TGGAGATTCT GCAGACGCTC ATTAAAGTGC TGATGGTATT CTCCATACTT 
ATAATCGCCT TTGGTCTGGC TTTCTATATA CTACTTTCAA AGATTATTGA CCCCCAACCG 
AACCACTTGT CCTTCTCCAA CATACCCATG TCCTTGCTGC GAACTTTCTC AATGATGCTG 
GGCGAGCTGG ACTTTGTGGG TACCTATGTG AACACCTACT ATCGGGATCA GTTGAAAGTG 
CCCATGACAT CCTTTTTGAT TTTGAGTGTC TTTATGATCC TTATGCCCAT TCTTCTGATG 
AACTTGCTCA TCGGTTTGGC CGTCGGCGAT ATTGAGTCAG TGCGTCGCAA TGCCCAGCTC 
AAGAGACTGG CCATGCAGGT GGTGCTCCAC ACGGAGCTGG AGAGGAAGTT GCCCCATGTC 
TGGCTGCAGC GAGTTGACAA GATGGAGCTG ATTGAGTATC CCAATGAAAC CAAGTGCAAG 
CTGGGCTTCT GCGATTTCAT CCTGCGCAAG TGGTTCTCGA ATCCATTCAC CGAGGATTCC 
TCCATGGACG TCATCTCCTT CGACAACAAT GATGACTACA TCAACGCAGA ATTGGAACGG 
CAGAGGCGAA AGTTGCGCGA CATAAGTCGC ATGCTGGAGC AACAGCACCA TCTGGTTCGG 
CTCATTGTCC AAAAGATGGA GATCAAGACG GAGGCGGATG ACGTGGACGA GGGTATATCC 
CCAAACGAGT TGCGATCCGT CGTCGGTTTG AGATCGGCAG GCGGAAATCG ATGGAACTCG 
CCGCGAGTCC GGAATAAACT CCGAGCCGCC CTGAGCTTCA ATAAGAGCAT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TrpA1 ( NM_001274673.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261602.1 Drosophila melanogaster transient receptor potential cation channel A1, transcript variant K (TrpA1), mRNA.

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CloneID	OFa20763
Clone ID Related Accession (Same CDS sequence)	NM_001274673.1
Accession Version	NM_001274673.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	transient receptor potential cation channel A1, isoform K
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCAAGC TCTACAACGG AGTCTACAGC GGTCAGTGCG GCGCCCTATC GCCACCTGAC 
CTCATGGAGG CCCAGCCGAA GCTACTTCCC AAGCCAAGGA GCAACAGCAG CGGCAGCACC 
GGCCGGAACA GCAAGTATTG GATATTTTCA ATGATAATCG AGCGCAGTGC GGGTCCCAAG 
CGGATTGAAA TCGATGGCGA TGATGCGGAC ACGCCGCTGG AGGCCATCCT GCCAGCCGAA 
CCGCCGGCGG AGGTCTGCCT CTTGCGTGAC AGCCCCTTCA GGATATTGCG GGCGGCTGAG 
TCCGGAAACC TTGACGACTT CAAGCGACTC TTCATGGCGG ACAACTCGCG CATTGCTTTA 
AAGGATGCGA AAGGACGAAC GGCTGCCCAT CAGGCGGCGG CCCGTAATAG GGTTAACATT 
TTGCGGTACA TTCGCGACCA GAATGGCGAC TTTAATGCGA AGGATAATGC CGGCAATACC 
CCGCTCCACA TCGCCGTGGA GAGCGATGCC TACGACGCTC TGGACTATCT ATTGTCCATC 
CCAGTGGATA CGGGAGTGCT GAACGAGAAG AAGCAGGCAC CAGTGCACTT GGCCACCGAG 
CTGAACAAAG TGAAGTCCCT TCGGGTGATG GGTCAGTACC GCAATGTCAT CGATATTCAG 
CAGGGCGGCG AACATGGACG TACCGCTCTG CACTTGGCCG CCATCTATGA TCACGAGGAG 
TGCGCTCGCA TCCTGATAAC TGAGTTCGAT GCATGCCCAC GTAAGCCCTG TAACAATGGT 
TATTATCCCA TACACGAAGC GGCCAAGAAT GCCAGCTCCA AGACAATGGA GGTCTTCTTC 
CAGTGGGGCG AGCAGCGCGG CTGCACCCGC GAGGAGATGA TATCCTTCTA CGACTCGGAG 
GGCAATGTGC CGCTCCATTC GGCTGTCCAT GGTGGCGACA TCAAGGCTGT GGAGCTGTGC 
CTCAAGTCCG GGGCCAAGAT ATCTACGCAG CAACACGATC TCTCGACGCC AGTGCACCTG 
GCTTGTGCCC AGGGAGCCAT AGACATTGTA AAGCTCATGT TCGAGATGCA GCCAATGGAG 
AAGCGACTAT GTCTGAGTTG CACGGATGTG CAGAAGATGA CGCCGCTGCA CTGCGCCTCC 
ATGTTCGATC ATCCGGACAT TGTGTCCTAT CTGGTGGCCG AGGGAGCGGA CATCAATGCC 
CTGGACAAGG AGCATCGCTC TCCGTTACTG CTGGCGGCAT CTCGTAGCGG TTGGAAAACA 
GTCCACCTCC TGATTCGCCT GGGGGCGTGC ATTAGTGTCA AGGACGCCGC CGCCCGCAAT 
GTGCTGCACT TCGTCATCAT GAACGGCGGC CGGCTGACGG ACTTCGCGGA GCAGGTGGCC 
AATTGCCAGA CGCAGGCGCA GCTGAAGCTG CTGCTCAACG AGAAGGACAG CATGGGCTGC 
TCGCCGCTGC ACTACGCCAG TCGGGATGGA CACATCCGTT CGTTGGAGAA CCTCATTCGA 
CTGGGAGCCT GCATCAACCT GAAGAACAAC AACAACGAGA GTCCGCTGCA CTTTGCCGCA 
CGCTACGGAA GATACAATAC GGTGCGGCAG CTCTTGGATT CCGAGAAGGG ATCCTTCATC 
ATCAACGAAA GTGACGGTGC AGGGATGACA CCACTGCACA TATCCTCGCA GCAAGGACAC 
ACGCGAGTGG TGCAGCTGCT ACTCAATCGA GGAGCCCTGC TCCATCGGGA CCACACCGGA 
CGCAATCCTC TCCAGCTAGC GGCCATGTCC GGATACACCG AGACCATCGA GCTGCTGCAC 
TCGGTGCACT CGCATCTGCT CGATCAGGTG GACAAGGATG GGAACACCGC TCTTCACCTG 
GCCACCATGG AGAATAAGCC CCATGCGATC TCCGTGCTGA TGTCTATGGG CTGTAAGCTG 
GTCTATAACG TTCTGGACAT GAGTGCCATT GACTATGCCA TCTACTACAA ATATCCGGAG 
GCTGCCCTGG CCATGGTCAC CCACGAGGAG CGGGCCAACG AGGTGATGGC TCTGCGTTCC 
GACAAGCATC CGTGCGTGAC CCTCGCCTTA ATTGCCTCCA TGCCCAAGGT ATTCGAGGCG 
GTGCAGGACA AGTGCATTAC CAAGGCCAAT TGCAAGAAGG ACTCGAAGAG TTTCTACACC 
ATGGTAACAC ATGGCAGGGT GGAGCTGCTG GCCCATCCGC TCAGTCAGAA GTATCTGCAG 
ATGAAGTGGA ACTCCTACGG CAAGTACTTT CACCTGGCCA ACCTGCTAAT CTACTCGATA 
TTCCTGGTCT TTGTAACCAT CTACTCTTCG CTGATGATGA ACAACATCGA ACTGAAGGCT 
GGGGACAACA AGACGATGAG TCAATACTGC AATATGGGAT GGGAGCAGCT GACCATGAAT 
CTCTCGCAGA ACCCGTCGGT GGCATCACAG ATTCGTTTGG ATTCCTGCGA GGAGCGTATA 
AATAGAACCA CTGCAATACT TTTCTGTGCG GTGGTCATCG TGGTCTATAT ACTGCTCAAC 
TCGATGCGGG AACTAATACA GATATACCAG CAGAAATTGC ACTATATCCT GGAGACAGTT 
AATTTGATAT CCTGGGTGCT GTACATCTCG GCTTTGGTGA TGGTAACACC GGCATTTCAG 
CCGGATGGAG GAATCAATAC CATTCATTAC TCGGCCGCTT CAATAGCAGT CTTTCTGTCG 
TGGTTCCGAT TGCTACTGTT CCTGCAAAGA TTCGACCAGG TCGGCATCTA TGTGGTCATG 
TTCTTGGAGA TTCTGCAGAC GCTCATTAAA GTGCTGATGG TATTCTCCAT ACTTATAATC 
GCCTTTGGTC TGGCTTTCTA TATACTACTT TCAAAGATTA TTGACCCCCA ACCGAACCAC 
TTGTCCTTCT CCAACATACC CATGTCCTTG CTGCGAACTT TCTCAATGAT GCTGGGCGAG 
CTGGACTTTG TGGGTACCTA TGTGAACACC TACTATCGGG ATCAGTTGAA AGTGCCCATG 
ACATCCTTTT TGATTTTGAG TGTCTTTATG ATCCTTATGC CCATTCTTCT GATGAACTTG 
CTCATCGGTT TGGCCGTCGG CGATATTGAG TCAGTGCGTC GCAATGCCCA GCTCAAGAGA 
CTGGCCATGC AGGTGGTGCT CCACACGGAG CTGGAGAGGA AGTTGCCCCA TGTCTGGCTG 
CAGCGAGTTG ACAAGATGGA GCTGATTGAG TATCCCAATG AAACCAAGTG CAAGCTGGGC 
TTCTGCGATT TCATCCTGCG CAAGTGGTTC TCGAATCCAT TCACCGAGGA TTCCTCCATG 
GACGTCATCT CCTTCGACAA CAATGATGAC TACATCAACG CAGAATTGGA ACGGCAGAGG 
CGAAAGTTGC GCGACATAAG TCGCATGCTG GAGCAACAGC ACCATCTGGT TCGGCTCATT 
GTCCAAAAGA TGGAGATCAA GACGGAGGCG GATGACGTGG ACGAGGGTAT ATCCCCAAAC 
GAGTTGCGAT CCGTCGTCGG TTTGAGATCG GCAGGCGGAA ATCGATGGAA CTCGCCGCGA 
GTCCGGAATA AACTCCGAGC CGCCCTGAGC TTCAATAAGA GCATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261602.1
CDS	181..3768
Translation	MPKLYNGVYSGQCGALSPPDLMEAQPKLLPKPRSNSSGSTGRNSKYWIFSMIIERSAGPKRIEIDGDDADTPLEAILPAEPPAEVCLLRDSPFRILRAAESGNLDDFKRLFMADNSRIALKDAKGRTAAHQAAARNRVNILRYIRDQNGDFNAKDNAGNTPLHIAVESDAYDALDYLLSIPVDTGVLNEKKQAPVHLATELNKVKSLRVMGQYRNVIDIQQGGEHGRTALHLAAIYDHEECARILITEFDACPRKPCNNGYYPIHEAAKNASSKTMEVFFQWGEQRGCTREEMISFYDSEGNVPLHSAVHGGDIKAVELCLKSGAKISTQQHDLSTPVHLACAQGAIDIVKLMFEMQPMEKRLCLSCTDVQKMTPLHCASMFDHPDIVSYLVAEGADINALDKEHRSPLLLAASRSGWKTVHLLIRLGACISVKDAAARNVLHFVIMNGGRLTDFAEQVANCQTQAQLKLLLNEKDSMGCSPLHYASRDGHIRSLENLIRLGACINLKNNNNESPLHFAARYGRYNTVRQLLDSEKGSFIINESDGAGMTPLHISSQQGHTRVVQLLLNRGALLHRDHTGRNPLQLAAMSGYTETIELLHSVHSHLLDQVDKDGNTALHLATMENKPHAISVLMSMGCKLVYNVLDMSAIDYAIYYKYPEAALAMVTHEERANEVMALRSDKHPCVTLALIASMPKVFEAVQDKCITKANCKKDSKSFYTMVTHGRVELLAHPLSQKYLQMKWNSYGKYFHLANLLIYSIFLVFVTIYSSLMMNNIELKAGDNKTMSQYCNMGWEQLTMNLSQNPSVASQIRLDSCEERINRTTAILFCAVVIVVYILLNSMRELIQIYQQKLHYILETVNLISWVLYISALVMVTPAFQPDGGINTIHYSAASIAVFLSWFRLLLFLQRFDQVGIYVVMFLEILQTLIKVLMVFSILIIAFGLAFYILLSKIIDPQPNHLSFSNIPMSLLRTFSMMLGELDFVGTYVNTYYRDQLKVPMTSFLILSVFMILMPILLMNLLIGLAVGDIESVRRNAQLKRLAMQVVLHTELERKLPHVWLQRVDKMELIEYPNETKCKLGFCDFILRKWFSNPFTEDSSMDVISFDNNDDYINAELERQRRKLRDISRMLEQQHHLVRLIVQKMEIKTEADDVDEGISPNELRSVVGLRSAGGNRWNSPRVRNKLRAALSFNKSM

Target ORF information:

RefSeq Version	NM_001274673.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster transient receptor potential cation channel A1, transcript variant K (TrpA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274673.1

ORF Insert Sequence:

ATGCCCAAGC TCTACAACGG AGTCTACAGC GGTCAGTGCG GCGCCCTATC GCCACCTGAC 
CTCATGGAGG CCCAGCCGAA GCTACTTCCC AAGCCAAGGA GCAACAGCAG CGGCAGCACC 
GGCCGGAACA GCAAGTATTG GATATTTTCA ATGATAATCG AGCGCAGTGC GGGTCCCAAG 
CGGATTGAAA TCGATGGCGA TGATGCGGAC ACGCCGCTGG AGGCCATCCT GCCAGCCGAA 
CCGCCGGCGG AGGTCTGCCT CTTGCGTGAC AGCCCCTTCA GGATATTGCG GGCGGCTGAG 
TCCGGAAACC TTGACGACTT CAAGCGACTC TTCATGGCGG ACAACTCGCG CATTGCTTTA 
AAGGATGCGA AAGGACGAAC GGCTGCCCAT CAGGCGGCGG CCCGTAATAG GGTTAACATT 
TTGCGGTACA TTCGCGACCA GAATGGCGAC TTTAATGCGA AGGATAATGC CGGCAATACC 
CCGCTCCACA TCGCCGTGGA GAGCGATGCC TACGACGCTC TGGACTATCT ATTGTCCATC 
CCAGTGGATA CGGGAGTGCT GAACGAGAAG AAGCAGGCAC CAGTGCACTT GGCCACCGAG 
CTGAACAAAG TGAAGTCCCT TCGGGTGATG GGTCAGTACC GCAATGTCAT CGATATTCAG 
CAGGGCGGCG AACATGGACG TACCGCTCTG CACTTGGCCG CCATCTATGA TCACGAGGAG 
TGCGCTCGCA TCCTGATAAC TGAGTTCGAT GCATGCCCAC GTAAGCCCTG TAACAATGGT 
TATTATCCCA TACACGAAGC GGCCAAGAAT GCCAGCTCCA AGACAATGGA GGTCTTCTTC 
CAGTGGGGCG AGCAGCGCGG CTGCACCCGC GAGGAGATGA TATCCTTCTA CGACTCGGAG 
GGCAATGTGC CGCTCCATTC GGCTGTCCAT GGTGGCGACA TCAAGGCTGT GGAGCTGTGC 
CTCAAGTCCG GGGCCAAGAT ATCTACGCAG CAACACGATC TCTCGACGCC AGTGCACCTG 
GCTTGTGCCC AGGGAGCCAT AGACATTGTA AAGCTCATGT TCGAGATGCA GCCAATGGAG 
AAGCGACTAT GTCTGAGTTG CACGGATGTG CAGAAGATGA CGCCGCTGCA CTGCGCCTCC 
ATGTTCGATC ATCCGGACAT TGTGTCCTAT CTGGTGGCCG AGGGAGCGGA CATCAATGCC 
CTGGACAAGG AGCATCGCTC TCCGTTACTG CTGGCGGCAT CTCGTAGCGG TTGGAAAACA 
GTCCACCTCC TGATTCGCCT GGGGGCGTGC ATTAGTGTCA AGGACGCCGC CGCCCGCAAT 
GTGCTGCACT TCGTCATCAT GAACGGCGGC CGGCTGACGG ACTTCGCGGA GCAGGTGGCC 
AATTGCCAGA CGCAGGCGCA GCTGAAGCTG CTGCTCAACG AGAAGGACAG CATGGGCTGC 
TCGCCGCTGC ACTACGCCAG TCGGGATGGA CACATCCGTT CGTTGGAGAA CCTCATTCGA 
CTGGGAGCCT GCATCAACCT GAAGAACAAC AACAACGAGA GTCCGCTGCA CTTTGCCGCA 
CGCTACGGAA GATACAATAC GGTGCGGCAG CTCTTGGATT CCGAGAAGGG ATCCTTCATC 
ATCAACGAAA GTGACGGTGC AGGGATGACA CCACTGCACA TATCCTCGCA GCAAGGACAC 
ACGCGAGTGG TGCAGCTGCT ACTCAATCGA GGAGCCCTGC TCCATCGGGA CCACACCGGA 
CGCAATCCTC TCCAGCTAGC GGCCATGTCC GGATACACCG AGACCATCGA GCTGCTGCAC 
TCGGTGCACT CGCATCTGCT CGATCAGGTG GACAAGGATG GGAACACCGC TCTTCACCTG 
GCCACCATGG AGAATAAGCC CCATGCGATC TCCGTGCTGA TGTCTATGGG CTGTAAGCTG 
GTCTATAACG TTCTGGACAT GAGTGCCATT GACTATGCCA TCTACTACAA ATATCCGGAG 
GCTGCCCTGG CCATGGTCAC CCACGAGGAG CGGGCCAACG AGGTGATGGC TCTGCGTTCC 
GACAAGCATC CGTGCGTGAC CCTCGCCTTA ATTGCCTCCA TGCCCAAGGT ATTCGAGGCG 
GTGCAGGACA AGTGCATTAC CAAGGCCAAT TGCAAGAAGG ACTCGAAGAG TTTCTACACC 
ATGGTAACAC ATGGCAGGGT GGAGCTGCTG GCCCATCCGC TCAGTCAGAA GTATCTGCAG 
ATGAAGTGGA ACTCCTACGG CAAGTACTTT CACCTGGCCA ACCTGCTAAT CTACTCGATA 
TTCCTGGTCT TTGTAACCAT CTACTCTTCG CTGATGATGA ACAACATCGA ACTGAAGGCT 
GGGGACAACA AGACGATGAG TCAATACTGC AATATGGGAT GGGAGCAGCT GACCATGAAT 
CTCTCGCAGA ACCCGTCGGT GGCATCACAG ATTCGTTTGG ATTCCTGCGA GGAGCGTATA 
AATAGAACCA CTGCAATACT TTTCTGTGCG GTGGTCATCG TGGTCTATAT ACTGCTCAAC 
TCGATGCGGG AACTAATACA GATATACCAG CAGAAATTGC ACTATATCCT GGAGACAGTT 
AATTTGATAT CCTGGGTGCT GTACATCTCG GCTTTGGTGA TGGTAACACC GGCATTTCAG 
CCGGATGGAG GAATCAATAC CATTCATTAC TCGGCCGCTT CAATAGCAGT CTTTCTGTCG 
TGGTTCCGAT TGCTACTGTT CCTGCAAAGA TTCGACCAGG TCGGCATCTA TGTGGTCATG 
TTCTTGGAGA TTCTGCAGAC GCTCATTAAA GTGCTGATGG TATTCTCCAT ACTTATAATC 
GCCTTTGGTC TGGCTTTCTA TATACTACTT TCAAAGATTA TTGACCCCCA ACCGAACCAC 
TTGTCCTTCT CCAACATACC CATGTCCTTG CTGCGAACTT TCTCAATGAT GCTGGGCGAG 
CTGGACTTTG TGGGTACCTA TGTGAACACC TACTATCGGG ATCAGTTGAA AGTGCCCATG 
ACATCCTTTT TGATTTTGAG TGTCTTTATG ATCCTTATGC CCATTCTTCT GATGAACTTG 
CTCATCGGTT TGGCCGTCGG CGATATTGAG TCAGTGCGTC GCAATGCCCA GCTCAAGAGA 
CTGGCCATGC AGGTGGTGCT CCACACGGAG CTGGAGAGGA AGTTGCCCCA TGTCTGGCTG 
CAGCGAGTTG ACAAGATGGA GCTGATTGAG TATCCCAATG AAACCAAGTG CAAGCTGGGC 
TTCTGCGATT TCATCCTGCG CAAGTGGTTC TCGAATCCAT TCACCGAGGA TTCCTCCATG 
GACGTCATCT CCTTCGACAA CAATGATGAC TACATCAACG CAGAATTGGA ACGGCAGAGG 
CGAAAGTTGC GCGACATAAG TCGCATGCTG GAGCAACAGC ACCATCTGGT TCGGCTCATT 
GTCCAAAAGA TGGAGATCAA GACGGAGGCG GATGACGTGG ACGAGGGTAT ATCCCCAAAC 
GAGTTGCGAT CCGTCGTCGG TTTGAGATCG GCAGGCGGAA ATCGATGGAA CTCGCCGCGA 
GTCCGGAATA AACTCCGAGC CGCCCTGAGC TTCAATAAGA GCATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TrpA1 ( NM_001274672.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261601.1 Drosophila melanogaster transient receptor potential cation channel A1, transcript variant H (TrpA1), mRNA.

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CloneID	OFa20764
Clone ID Related Accession (Same CDS sequence)	NM_001274672.1
Accession Version	NM_001274672.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	transient receptor potential cation channel A1, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCAAGC TCTACAACGG AGTCTACAGC GGTCAGTGCG GCGCCCTATC GCCACCTGAC 
CTCATGGAGG CCCAGCCGAA GCTACTTCCC AAGCCAAGGA GCAACAGCAG CGGCAGCACC 
GGCCGGAACA GCAAGTATTG GATATTTTCA ATGATAATCG AGCGCAGTGC GGGTCCCAAG 
CGGATTGAAA TCGATGGCGA TGATGCGGAC ACGCCGCTGG AGGCCATCCT GCCAGCCGAA 
CCGCCGGCGG AGGTCTGCCT CTTGCGTGAC AGCCCCTTCA GGATATTGCG GGCGGCTGAG 
TCCGGAAACC TTGACGACTT CAAGCGACTC TTCATGGCGG ACAACTCGCG CATTGCTTTA 
AAGGATGCGA AAGGACGAAC GGCTGCCCAT CAGGCGGCGG CCCGTAATAG GGTTAACATT 
TTGCGGTACA TTCGCGACCA GAATGGCGAC TTTAATGCGA AGGATAATGC CGGCAATACC 
CCGCTCCACA TCGCCGTGGA GAGCGATGCC TACGACGCTC TGGACTATCT ATTGTCCATC 
CCAGTGGATA CGGGAGTGCT GAACGAGAAG AAGCAGGCAC CAGTGCACTT GGCCACCGAG 
CTGAACAAAG TGAAGTCCCT TCGGGTGATG GGTCAGTACC GCAATGTCAT CGATATTCAG 
CAGGGCGGCG AACATGGACG TACCGCTCTG CACTTGGCCG CCATCTATGA TCACGAGGAG 
TGCGCTCGCA TCCTGATAAC TGAGTTCGAT GCATGCCCAC GTAAGCCCTG TAACAATGGT 
TATTATCCCA TACACGAAGC GGCCAAGAAT GCCAGCTCCA AGACAATGGA GGTCTTCTTC 
CAGTGGGGCG AGCAGCGCGG CTGCACCCGC GAGGAGATGA TATCCTTCTA CGACTCGGAG 
GGCAATGTGC CGCTCCATTC GGCTGTCCAT GGTGGCGACA TCAAGGCTGT GGAGCTGTGC 
CTCAAGTCCG GGGCCAAGAT ATCTACGCAG CAACACGATC TCTCGACGCC AGTGCACCTG 
GCTTGTGCCC AGGGAGCCAT AGACATTGTA AAGCTCATGT TCGAGATGCA GCCAATGGAG 
AAGCGACTAT GTCTGAGTTG CACGGATGTG CAGAAGATGA CGCCGCTGCA CTGCGCCTCC 
ATGTTCGATC ATCCGGACAT TGTGTCCTAT CTGGTGGCCG AGGGAGCGGA CATCAATGCC 
CTGGACAAGG AGCATCGCTC TCCGTTACTG CTGGCGGCAT CTCGTAGCGG TTGGAAAACA 
GTCCACCTCC TGATTCGCCT GGGGGCGTGC ATTAGTGTCA AGGACGCCGC CGCCCGCAAT 
GTGCTGCACT TCGTCATCAT GAACGGCGGC CGGCTGACGG ACTTCGCGGA GCAGGTGGCC 
AATTGCCAGA CGCAGGCGCA GCTGAAGCTG CTGCTCAACG AGAAGGACAG CATGGGCTGC 
TCGCCGCTGC ACTACGCCAG TCGGGATGGA CACATCCGTT CGTTGGAGAA CCTCATTCGA 
CTGGGAGCCT GCATCAACCT GAAGAACAAC AACAACGAGA GTCCGCTGCA CTTTGCCGCA 
CGCTACGGAA GATACAATAC GGTGCGGCAG CTCTTGGATT CCGAGAAGGG ATCCTTCATC 
ATCAACGAAA GTGACGGTGC AGGGATGACA CCACTGCACA TATCCTCGCA GCAAGGACAC 
ACGCGAGTGG TGCAGCTGCT ACTCAATCGA GGAGCCCTGC TCCATCGGGA CCACACCGGA 
CGCAATCCTC TCCAGCTAGC GGCCATGTCC GGATACACCG AGACCATCGA GCTGCTGCAC 
TCGGTGCACT CGCATCTGCT CGATCAGGTG GACAAGGATG GGAACACCGC TCTTCACCTG 
GCCACCATGG AGAATAAGCC CCATGCGATC TCCGTGCTGA TGTCTATGGG CTGTAAGCTG 
GTCTATAACG TTCTGGACAT GAGTGCCATT GACTATGCCA TCTACTACAA ATATCCGGAG 
GCTGCCCTGG CCATGGTCAC CCACGAGGAG CGGGCCAACG AGGTGATGGC TCTGCGTTCC 
GACAAGCATC CGTGCGTGAC CCTCGCCTTA ATTGCCTCCA TGCCCAAGGT ATTCGAGGCG 
GTGCAGGACA AGTGCATTAC CAAGGCCAAT TGCAAGAAGG ACTCGAAGAG TTTCTACATA 
AAATATTCGT TTTGGCCCTA CCAAAAGACA CCCGAACAGA TTGAGGCCAA GCGCAAAGAG 
TTCAATGACC CCAAGTGGCG ACCCGCGCCT TTGGCCGTGG TGAACACCAT GGTAACACAT 
GGCAGGGTGG AGCTGCTGGC CCATCCGCTC AGTCAGAAGT ATCTGCAGAT GAAGTGGAAC 
TCCTACGGCA AGTACTTTCA CCTGGCCAAC CTGCTAATCT ACTCGATATT CCTGGTCTTT 
GTAACCATCT ACTCTTCGCT GATGATGAAC AACATCGAAC TGAAGGCTGG GGACAACAAG 
ACGATGAGTC AATACTGCAA TATGGGATGG GAGCAGCTGA CCATGAATCT CTCGCAGAAC 
CCGTCGGTGG CATCACAGAT TCGTTTGGAT TCCTGCGAGG AGCGTATAAA TAGAACCACT 
GCAATACTTT TCTGTGCGGT GGTCATCGTG GTCTATATAC TGCTCAACTC GATGCGGGAA 
CTAATACAGA TATACCAGCA GAAATTGCAC TATATCCTGG AGACAGTTAA TTTGATATCC 
TGGGTGCTGT ACATCTCGGC TTTGGTGATG GTAACACCGG CATTTCAGCC GGATGGAGGA 
ATCAATACCA TTCATTACTC GGCCGCTTCA ATAGCAGTCT TTCTGTCGTG GTTCCGATTG 
CTACTGTTCC TGCAAAGATT CGACCAGGTC GGCATCTATG TGGTCATGTT CTTGGAGATT 
CTGCAGACGC TCATTAAAGT GCTGATGGTA TTCTCCATAC TTATAATCGC CTTTGGTCTG 
GCTTTCTATA TACTACTTTC AAAGATTATT GACCCCCAAC CGAACCACTT GTCCTTCTCC 
AACATACCCA TGTCCTTGCT GCGAACTTTC TCAATGATGC TGGGCGAGCT GGACTTTGTG 
GGTACCTATG TGAACACCTA CTATCGGGAT CAGTTGAAAG TGCCCATGAC ATCCTTTTTG 
ATTTTGAGTG TCTTTATGAT CCTTATGCCC ATTCTTCTGA TGAACTTGCT CATCGGTTTG 
GCCGTCGGCG ATATTGAGTC AGTGCGTCGC AATGCCCAGC TCAAGAGACT GGCCATGCAG 
GTGGTGCTCC ACACGGAGCT GGAGAGGAAG TTGCCCCATG TCTGGCTGCA GCGAGTTGAC 
AAGATGGAGC TGATTGAGTA TCCCAATGAA ACCAAGTGCA AGCTGGGCTT CTGCGATTTC 
ATCCTGCGCA AGTGGTTCTC GAATCCATTC ACCGAGGATT CCTCCATGGA CGTCATCTCC 
TTCGACAACA ATGATGACTA CATCAACGCA GAATTGGAAC GGCAGAGGCG AAAGTTGCGC 
GACATAAGTC GCATGCTGGA GCAACAGCAC CATCTGGTTC GGCTCATTGT CCAAAAGATG 
GAGATCAAGA CGGAGGCGGA TGACGTGGAC GAGGGTATAT CCCCAAACGA GTTGCGATCC 
GTCGTCGGTT TGAGATCGGC AGGCGGAAAT CGATGGAACT CGCCGCGAGT CCGGAATAAA 
CTCCGAGCCG CCCTGAGCTT CAATAAGAGC ATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261601.1
CDS	181..3876
Translation	MPKLYNGVYSGQCGALSPPDLMEAQPKLLPKPRSNSSGSTGRNSKYWIFSMIIERSAGPKRIEIDGDDADTPLEAILPAEPPAEVCLLRDSPFRILRAAESGNLDDFKRLFMADNSRIALKDAKGRTAAHQAAARNRVNILRYIRDQNGDFNAKDNAGNTPLHIAVESDAYDALDYLLSIPVDTGVLNEKKQAPVHLATELNKVKSLRVMGQYRNVIDIQQGGEHGRTALHLAAIYDHEECARILITEFDACPRKPCNNGYYPIHEAAKNASSKTMEVFFQWGEQRGCTREEMISFYDSEGNVPLHSAVHGGDIKAVELCLKSGAKISTQQHDLSTPVHLACAQGAIDIVKLMFEMQPMEKRLCLSCTDVQKMTPLHCASMFDHPDIVSYLVAEGADINALDKEHRSPLLLAASRSGWKTVHLLIRLGACISVKDAAARNVLHFVIMNGGRLTDFAEQVANCQTQAQLKLLLNEKDSMGCSPLHYASRDGHIRSLENLIRLGACINLKNNNNESPLHFAARYGRYNTVRQLLDSEKGSFIINESDGAGMTPLHISSQQGHTRVVQLLLNRGALLHRDHTGRNPLQLAAMSGYTETIELLHSVHSHLLDQVDKDGNTALHLATMENKPHAISVLMSMGCKLVYNVLDMSAIDYAIYYKYPEAALAMVTHEERANEVMALRSDKHPCVTLALIASMPKVFEAVQDKCITKANCKKDSKSFYIKYSFWPYQKTPEQIEAKRKEFNDPKWRPAPLAVVNTMVTHGRVELLAHPLSQKYLQMKWNSYGKYFHLANLLIYSIFLVFVTIYSSLMMNNIELKAGDNKTMSQYCNMGWEQLTMNLSQNPSVASQIRLDSCEERINRTTAILFCAVVIVVYILLNSMRELIQIYQQKLHYILETVNLISWVLYISALVMVTPAFQPDGGINTIHYSAASIAVFLSWFRLLLFLQRFDQVGIYVVMFLEILQTLIKVLMVFSILIIAFGLAFYILLSKIIDPQPNHLSFSNIPMSLLRTFSMMLGELDFVGTYVNTYYRDQLKVPMTSFLILSVFMILMPILLMNLLIGLAVGDIESVRRNAQLKRLAMQVVLHTELERKLPHVWLQRVDKMELIEYPNETKCKLGFCDFILRKWFSNPFTEDSSMDVISFDNNDDYINAELERQRRKLRDISRMLEQQHHLVRLIVQKMEIKTEADDVDEGISPNELRSVVGLRSAGGNRWNSPRVRNKLRAALSFNKSM

Target ORF information:

RefSeq Version	NM_001274672.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster transient receptor potential cation channel A1, transcript variant H (TrpA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274672.1

ORF Insert Sequence:

ATGCCCAAGC TCTACAACGG AGTCTACAGC GGTCAGTGCG GCGCCCTATC GCCACCTGAC 
CTCATGGAGG CCCAGCCGAA GCTACTTCCC AAGCCAAGGA GCAACAGCAG CGGCAGCACC 
GGCCGGAACA GCAAGTATTG GATATTTTCA ATGATAATCG AGCGCAGTGC GGGTCCCAAG 
CGGATTGAAA TCGATGGCGA TGATGCGGAC ACGCCGCTGG AGGCCATCCT GCCAGCCGAA 
CCGCCGGCGG AGGTCTGCCT CTTGCGTGAC AGCCCCTTCA GGATATTGCG GGCGGCTGAG 
TCCGGAAACC TTGACGACTT CAAGCGACTC TTCATGGCGG ACAACTCGCG CATTGCTTTA 
AAGGATGCGA AAGGACGAAC GGCTGCCCAT CAGGCGGCGG CCCGTAATAG GGTTAACATT 
TTGCGGTACA TTCGCGACCA GAATGGCGAC TTTAATGCGA AGGATAATGC CGGCAATACC 
CCGCTCCACA TCGCCGTGGA GAGCGATGCC TACGACGCTC TGGACTATCT ATTGTCCATC 
CCAGTGGATA CGGGAGTGCT GAACGAGAAG AAGCAGGCAC CAGTGCACTT GGCCACCGAG 
CTGAACAAAG TGAAGTCCCT TCGGGTGATG GGTCAGTACC GCAATGTCAT CGATATTCAG 
CAGGGCGGCG AACATGGACG TACCGCTCTG CACTTGGCCG CCATCTATGA TCACGAGGAG 
TGCGCTCGCA TCCTGATAAC TGAGTTCGAT GCATGCCCAC GTAAGCCCTG TAACAATGGT 
TATTATCCCA TACACGAAGC GGCCAAGAAT GCCAGCTCCA AGACAATGGA GGTCTTCTTC 
CAGTGGGGCG AGCAGCGCGG CTGCACCCGC GAGGAGATGA TATCCTTCTA CGACTCGGAG 
GGCAATGTGC CGCTCCATTC GGCTGTCCAT GGTGGCGACA TCAAGGCTGT GGAGCTGTGC 
CTCAAGTCCG GGGCCAAGAT ATCTACGCAG CAACACGATC TCTCGACGCC AGTGCACCTG 
GCTTGTGCCC AGGGAGCCAT AGACATTGTA AAGCTCATGT TCGAGATGCA GCCAATGGAG 
AAGCGACTAT GTCTGAGTTG CACGGATGTG CAGAAGATGA CGCCGCTGCA CTGCGCCTCC 
ATGTTCGATC ATCCGGACAT TGTGTCCTAT CTGGTGGCCG AGGGAGCGGA CATCAATGCC 
CTGGACAAGG AGCATCGCTC TCCGTTACTG CTGGCGGCAT CTCGTAGCGG TTGGAAAACA 
GTCCACCTCC TGATTCGCCT GGGGGCGTGC ATTAGTGTCA AGGACGCCGC CGCCCGCAAT 
GTGCTGCACT TCGTCATCAT GAACGGCGGC CGGCTGACGG ACTTCGCGGA GCAGGTGGCC 
AATTGCCAGA CGCAGGCGCA GCTGAAGCTG CTGCTCAACG AGAAGGACAG CATGGGCTGC 
TCGCCGCTGC ACTACGCCAG TCGGGATGGA CACATCCGTT CGTTGGAGAA CCTCATTCGA 
CTGGGAGCCT GCATCAACCT GAAGAACAAC AACAACGAGA GTCCGCTGCA CTTTGCCGCA 
CGCTACGGAA GATACAATAC GGTGCGGCAG CTCTTGGATT CCGAGAAGGG ATCCTTCATC 
ATCAACGAAA GTGACGGTGC AGGGATGACA CCACTGCACA TATCCTCGCA GCAAGGACAC 
ACGCGAGTGG TGCAGCTGCT ACTCAATCGA GGAGCCCTGC TCCATCGGGA CCACACCGGA 
CGCAATCCTC TCCAGCTAGC GGCCATGTCC GGATACACCG AGACCATCGA GCTGCTGCAC 
TCGGTGCACT CGCATCTGCT CGATCAGGTG GACAAGGATG GGAACACCGC TCTTCACCTG 
GCCACCATGG AGAATAAGCC CCATGCGATC TCCGTGCTGA TGTCTATGGG CTGTAAGCTG 
GTCTATAACG TTCTGGACAT GAGTGCCATT GACTATGCCA TCTACTACAA ATATCCGGAG 
GCTGCCCTGG CCATGGTCAC CCACGAGGAG CGGGCCAACG AGGTGATGGC TCTGCGTTCC 
GACAAGCATC CGTGCGTGAC CCTCGCCTTA ATTGCCTCCA TGCCCAAGGT ATTCGAGGCG 
GTGCAGGACA AGTGCATTAC CAAGGCCAAT TGCAAGAAGG ACTCGAAGAG TTTCTACATA 
AAATATTCGT TTTGGCCCTA CCAAAAGACA CCCGAACAGA TTGAGGCCAA GCGCAAAGAG 
TTCAATGACC CCAAGTGGCG ACCCGCGCCT TTGGCCGTGG TGAACACCAT GGTAACACAT 
GGCAGGGTGG AGCTGCTGGC CCATCCGCTC AGTCAGAAGT ATCTGCAGAT GAAGTGGAAC 
TCCTACGGCA AGTACTTTCA CCTGGCCAAC CTGCTAATCT ACTCGATATT CCTGGTCTTT 
GTAACCATCT ACTCTTCGCT GATGATGAAC AACATCGAAC TGAAGGCTGG GGACAACAAG 
ACGATGAGTC AATACTGCAA TATGGGATGG GAGCAGCTGA CCATGAATCT CTCGCAGAAC 
CCGTCGGTGG CATCACAGAT TCGTTTGGAT TCCTGCGAGG AGCGTATAAA TAGAACCACT 
GCAATACTTT TCTGTGCGGT GGTCATCGTG GTCTATATAC TGCTCAACTC GATGCGGGAA 
CTAATACAGA TATACCAGCA GAAATTGCAC TATATCCTGG AGACAGTTAA TTTGATATCC 
TGGGTGCTGT ACATCTCGGC TTTGGTGATG GTAACACCGG CATTTCAGCC GGATGGAGGA 
ATCAATACCA TTCATTACTC GGCCGCTTCA ATAGCAGTCT TTCTGTCGTG GTTCCGATTG 
CTACTGTTCC TGCAAAGATT CGACCAGGTC GGCATCTATG TGGTCATGTT CTTGGAGATT 
CTGCAGACGC TCATTAAAGT GCTGATGGTA TTCTCCATAC TTATAATCGC CTTTGGTCTG 
GCTTTCTATA TACTACTTTC AAAGATTATT GACCCCCAAC CGAACCACTT GTCCTTCTCC 
AACATACCCA TGTCCTTGCT GCGAACTTTC TCAATGATGC TGGGCGAGCT GGACTTTGTG 
GGTACCTATG TGAACACCTA CTATCGGGAT CAGTTGAAAG TGCCCATGAC ATCCTTTTTG 
ATTTTGAGTG TCTTTATGAT CCTTATGCCC ATTCTTCTGA TGAACTTGCT CATCGGTTTG 
GCCGTCGGCG ATATTGAGTC AGTGCGTCGC AATGCCCAGC TCAAGAGACT GGCCATGCAG 
GTGGTGCTCC ACACGGAGCT GGAGAGGAAG TTGCCCCATG TCTGGCTGCA GCGAGTTGAC 
AAGATGGAGC TGATTGAGTA TCCCAATGAA ACCAAGTGCA AGCTGGGCTT CTGCGATTTC 
ATCCTGCGCA AGTGGTTCTC GAATCCATTC ACCGAGGATT CCTCCATGGA CGTCATCTCC 
TTCGACAACA ATGATGACTA CATCAACGCA GAATTGGAAC GGCAGAGGCG AAAGTTGCGC 
GACATAAGTC GCATGCTGGA GCAACAGCAC CATCTGGTTC GGCTCATTGT CCAAAAGATG 
GAGATCAAGA CGGAGGCGGA TGACGTGGAC GAGGGTATAT CCCCAAACGA GTTGCGATCC 
GTCGTCGGTT TGAGATCGGC AGGCGGAAAT CGATGGAACT CGCCGCGAGT CCGGAATAAA 
CTCCGAGCCG CCCTGAGCTT CAATAAGAGC ATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TrpA1 ( NM_001274671.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261600.1 Drosophila melanogaster transient receptor potential cation channel A1, transcript variant G (TrpA1), mRNA.

Price & Availability↑

CloneID	OFa20765
Clone ID Related Accession (Same CDS sequence)	NM_001274671.1
Accession Version	NM_001274671.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	transient receptor potential cation channel A1, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCAAGC TCTACAACGG AGTCTACAGC GGTCAGTGCG GCGCCCTATC GCCACCTGAC 
CTCATGGAGG CCCAGCCGAA GCTACTTCCC AAGCCAAGGA GCAACAGCAG CGGCAGCACC 
GGCCGGAACA GCAAGTATTG GATATTTTCA ATGATAATCG AGCGCAGTGC GGGTCCCAAG 
CGGATTGAAA TCGATGGCGA TGATGCGGAC ACGCCGCTGG AGGCCATCCT GCCAGCCGAA 
CCGCCGGCGG AGGTCTGCCT CTTGCGTGAC AGCCCCTTCA GGATATTGCG GGCGGCTGAG 
TCCGGAAACC TTGACGACTT CAAGCGACTC TTCATGGCGG ACAACTCGCG CATTGCTTTA 
AAGGATGCGA AAGGACGAAC GGCTGCCCAT CAGGCGGCGG CCCGTAATAG GGTTAACATT 
TTGCGGTACA TTCGCGACCA GAATGGCGAC TTTAATGCGA AGGATAATGC CGGCAATACC 
CCGCTCCACA TCGCCGTGGA GAGCGATGCC TACGACGCTC TGGACTATCT ATTGTCCATC 
CCAGTGGATA CGGGAGTGCT GAACGAGAAG AAGCAGGCAC CAGTGCACTT GGCCACCGAG 
CTGAACAAAG TGAAGTCCCT TCGGGTGATG GGTCAGTACC GCAATGTCAT CGATATTCAG 
CAGGGCGGCG AACATGGACG TACCGCTCTG CACTTGGCCG CCATCTATGA TCACGAGGAG 
TGCGCTCGCA TCCTGATAAC TGAGTTCGAT GCATGCCCAC GTAAGCCCTG TAACAATGGT 
TATTATCCCA TACACGAAGC GGCCAAGAAT GCCAGCTCCA AGACAATGGA GGTCTTCTTC 
CAGTGGGGCG AGCAGCGCGG CTGCACCCGC GAGGAGATGA TATCCTTCTA CGACTCGGAG 
GGCAATGTGC CGCTCCATTC GGCTGTCCAT GGTGGCGACA TCAAGGCTGT GGAGCTGTGC 
CTCAAGTCCG GGGCCAAGAT ATCTACGCAG CAACACGATC TCTCGACGCC AGTGCACCTG 
GCTTGTGCCC AGGGAGCCAT AGACATTGTA AAGCTCATGT TCGAGATGCA GCCAATGGAG 
AAGCGACTAT GTCTGAGTTG CACGGATGTG CAGAAGATGA CGCCGCTGCA CTGCGCCTCC 
ATGTTCGATC ATCCGGACAT TGTGTCCTAT CTGGTGGCCG AGGGAGCGGA CATCAATGCC 
CTGGACAAGG AGCATCGCTC TCCGTTACTG CTGGCGGCAT CTCGTAGCGG TTGGAAAACA 
GTCCACCTCC TGATTCGCCT GGGGGCGTGC ATTAGTGTCA AGGACGCCGC CGCCCGCAAT 
GTGCTGCACT TCGTCATCAT GAACGGCGGC CGGCTGACGG ACTTCGCGGA GCAGGTGGCC 
AATTGCCAGA CGCAGGCGCA GCTGAAGCTG CTGCTCAACG AGAAGGACAG CATGGGCTGC 
TCGCCGCTGC ACTACGCCAG TCGGGATGGA CACATCCGTT CGTTGGAGAA CCTCATTCGA 
CTGGGAGCCT GCATCAACCT GAAGAACAAC AACAACGAGA GTCCGCTGCA CTTTGCCGCA 
CGCTACGGAA GATACAATAC GGTGCGGCAG CTCTTGGATT CCGAGAAGGG ATCCTTCATC 
ATCAACGAAA GTGACGGTGC AGGGATGACA CCACTGCACA TATCCTCGCA GCAAGGACAC 
ACGCGAGTGG TGCAGCTGCT ACTCAATCGA GGAGCCCTGC TCCATCGGGA CCACACCGGA 
CGCAATCCTC TCCAGCTAGC GGCCATGTCC GGATACACCG AGACCATCGA GCTGCTGCAC 
TCGGTGCACT CGCATCTGCT CGATCAGGTG GACAAGGATG GGAACACCGC TCTTCACCTG 
GCCACCATGG AGAATAAGCC CCATGCGATC TCCGTGCTGA TGTCTATGGG CTGTAAGCTG 
GTCTATAACG TTCTGGACAT GAGTGCCATT GACTATGCCA TCTACTACAA ATATCCGGAG 
GCTGCCCTGG CCATGGTCAC CCACGAGGAG CGGGCCAACG AGGTGATGGC TCTGCGTTCC 
GACAAGCATC CGTGCGTGAC CCTCGCCTTA ATTGCCTCCA TGCCCAAGGT ATTCGAGGCG 
GTGCAGGACA AGTGCATTAC CAAGGCCAAT TGCAAGAAGG ACTCGAAGAG TTTCTACATC 
AAATACTCTT TCGCATTCTT GCAATGCCCC TTTATGTTTG CCAAGATTGA TGAGAAAACC 
GGAGAGTCGA TTACGACCGC CAGTCCCATT CCGTTGCCGG CTTTGAATAC CATGGTAACA 
CATGGCAGGG TGGAGCTGCT GGCCCATCCG CTCAGTCAGA AGTATCTGCA GATGAAGTGG 
AACTCCTACG GCAAGTACTT TCACCTGGCC AACCTGCTAA TCTACTCGAT ATTCCTGGTC 
TTTGTAACCA TCTACTCTTC GCTGATGATG AACAACATCG AACTGAAGGC TGGGGACAAC 
AAGACGATGA GTCAATACTG CAATATGGGA TGGGAGCAGC TGACCATGAA TCTCTCGCAG 
AACCCGTCGG TGGCATCACA GATTCGTTTG GATTCCTGCG AGGAGCGTAT AAATAGAACC 
ACTGCAATAC TTTTCTGTGC GGTGGTCATC GTGGTCTATA TACTGCTCAA CTCGATGCGG 
GAACTAATAC AGATATACCA GCAGAAATTG CACTATATCC TGGAGACAGT TAATTTGATA 
TCCTGGGTGC TGTACATCTC GGCTTTGGTG ATGGTAACAC CGGCATTTCA GCCGGATGGA 
GGAATCAATA CCATTCATTA CTCGGCCGCT TCAATAGCAG TCTTTCTGTC GTGGTTCCGA 
TTGCTACTGT TCCTGCAAAG ATTCGACCAG GTCGGCATCT ATGTGGTCAT GTTCTTGGAG 
ATTCTGCAGA CGCTCATTAA AGTGCTGATG GTATTCTCCA TACTTATAAT CGCCTTTGGT 
CTGGCTTTCT ATATACTACT TTCAAAGATT ATTGACCCCC AACCGAACCA CTTGTCCTTC 
TCCAACATAC CCATGTCCTT GCTGCGAACT TTCTCAATGA TGCTGGGCGA GCTGGACTTT 
GTGGGTACCT ATGTGAACAC CTACTATCGG GATCAGTTGA AAGTGCCCAT GACATCCTTT 
TTGATTTTGA GTGTCTTTAT GATCCTTATG CCCATTCTTC TGATGAACTT GCTCATCGGT 
TTGGCCGTCG GCGATATTGA GTCAGTGCGT CGCAATGCCC AGCTCAAGAG ACTGGCCATG 
CAGGTGGTGC TCCACACGGA GCTGGAGAGG AAGTTGCCCC ATGTCTGGCT GCAGCGAGTT 
GACAAGATGG AGCTGATTGA GTATCCCAAT GAAACCAAGT GCAAGCTGGG CTTCTGCGAT 
TTCATCCTGC GCAAGTGGTT CTCGAATCCA TTCACCGAGG ATTCCTCCAT GGACGTCATC 
TCCTTCGACA ACAATGATGA CTACATCAAC GCAGAATTGG AACGGCAGAG GCGAAAGTTG 
CGCGACATAA GTCGCATGCT GGAGCAACAG CACCATCTGG TTCGGCTCAT TGTCCAAAAG 
ATGGAGATCA AGACGGAGGC GGATGACGTG GACGAGGGTA TATCCCCAAA CGAGTTGCGA 
TCCGTCGTCG GTTTGAGATC GGCAGGCGGA AATCGATGGA ACTCGCCGCG AGTCCGGAAT 
AAACTCCGAG CCGCCCTGAG CTTCAATAAG AGCATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261600.1
CDS	181..3879
Translation	MPKLYNGVYSGQCGALSPPDLMEAQPKLLPKPRSNSSGSTGRNSKYWIFSMIIERSAGPKRIEIDGDDADTPLEAILPAEPPAEVCLLRDSPFRILRAAESGNLDDFKRLFMADNSRIALKDAKGRTAAHQAAARNRVNILRYIRDQNGDFNAKDNAGNTPLHIAVESDAYDALDYLLSIPVDTGVLNEKKQAPVHLATELNKVKSLRVMGQYRNVIDIQQGGEHGRTALHLAAIYDHEECARILITEFDACPRKPCNNGYYPIHEAAKNASSKTMEVFFQWGEQRGCTREEMISFYDSEGNVPLHSAVHGGDIKAVELCLKSGAKISTQQHDLSTPVHLACAQGAIDIVKLMFEMQPMEKRLCLSCTDVQKMTPLHCASMFDHPDIVSYLVAEGADINALDKEHRSPLLLAASRSGWKTVHLLIRLGACISVKDAAARNVLHFVIMNGGRLTDFAEQVANCQTQAQLKLLLNEKDSMGCSPLHYASRDGHIRSLENLIRLGACINLKNNNNESPLHFAARYGRYNTVRQLLDSEKGSFIINESDGAGMTPLHISSQQGHTRVVQLLLNRGALLHRDHTGRNPLQLAAMSGYTETIELLHSVHSHLLDQVDKDGNTALHLATMENKPHAISVLMSMGCKLVYNVLDMSAIDYAIYYKYPEAALAMVTHEERANEVMALRSDKHPCVTLALIASMPKVFEAVQDKCITKANCKKDSKSFYIKYSFAFLQCPFMFAKIDEKTGESITTASPIPLPALNTMVTHGRVELLAHPLSQKYLQMKWNSYGKYFHLANLLIYSIFLVFVTIYSSLMMNNIELKAGDNKTMSQYCNMGWEQLTMNLSQNPSVASQIRLDSCEERINRTTAILFCAVVIVVYILLNSMRELIQIYQQKLHYILETVNLISWVLYISALVMVTPAFQPDGGINTIHYSAASIAVFLSWFRLLLFLQRFDQVGIYVVMFLEILQTLIKVLMVFSILIIAFGLAFYILLSKIIDPQPNHLSFSNIPMSLLRTFSMMLGELDFVGTYVNTYYRDQLKVPMTSFLILSVFMILMPILLMNLLIGLAVGDIESVRRNAQLKRLAMQVVLHTELERKLPHVWLQRVDKMELIEYPNETKCKLGFCDFILRKWFSNPFTEDSSMDVISFDNNDDYINAELERQRRKLRDISRMLEQQHHLVRLIVQKMEIKTEADDVDEGISPNELRSVVGLRSAGGNRWNSPRVRNKLRAALSFNKSM

Target ORF information:

RefSeq Version	NM_001274671.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster transient receptor potential cation channel A1, transcript variant G (TrpA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274671.1

ORF Insert Sequence:

ATGCCCAAGC TCTACAACGG AGTCTACAGC GGTCAGTGCG GCGCCCTATC GCCACCTGAC 
CTCATGGAGG CCCAGCCGAA GCTACTTCCC AAGCCAAGGA GCAACAGCAG CGGCAGCACC 
GGCCGGAACA GCAAGTATTG GATATTTTCA ATGATAATCG AGCGCAGTGC GGGTCCCAAG 
CGGATTGAAA TCGATGGCGA TGATGCGGAC ACGCCGCTGG AGGCCATCCT GCCAGCCGAA 
CCGCCGGCGG AGGTCTGCCT CTTGCGTGAC AGCCCCTTCA GGATATTGCG GGCGGCTGAG 
TCCGGAAACC TTGACGACTT CAAGCGACTC TTCATGGCGG ACAACTCGCG CATTGCTTTA 
AAGGATGCGA AAGGACGAAC GGCTGCCCAT CAGGCGGCGG CCCGTAATAG GGTTAACATT 
TTGCGGTACA TTCGCGACCA GAATGGCGAC TTTAATGCGA AGGATAATGC CGGCAATACC 
CCGCTCCACA TCGCCGTGGA GAGCGATGCC TACGACGCTC TGGACTATCT ATTGTCCATC 
CCAGTGGATA CGGGAGTGCT GAACGAGAAG AAGCAGGCAC CAGTGCACTT GGCCACCGAG 
CTGAACAAAG TGAAGTCCCT TCGGGTGATG GGTCAGTACC GCAATGTCAT CGATATTCAG 
CAGGGCGGCG AACATGGACG TACCGCTCTG CACTTGGCCG CCATCTATGA TCACGAGGAG 
TGCGCTCGCA TCCTGATAAC TGAGTTCGAT GCATGCCCAC GTAAGCCCTG TAACAATGGT 
TATTATCCCA TACACGAAGC GGCCAAGAAT GCCAGCTCCA AGACAATGGA GGTCTTCTTC 
CAGTGGGGCG AGCAGCGCGG CTGCACCCGC GAGGAGATGA TATCCTTCTA CGACTCGGAG 
GGCAATGTGC CGCTCCATTC GGCTGTCCAT GGTGGCGACA TCAAGGCTGT GGAGCTGTGC 
CTCAAGTCCG GGGCCAAGAT ATCTACGCAG CAACACGATC TCTCGACGCC AGTGCACCTG 
GCTTGTGCCC AGGGAGCCAT AGACATTGTA AAGCTCATGT TCGAGATGCA GCCAATGGAG 
AAGCGACTAT GTCTGAGTTG CACGGATGTG CAGAAGATGA CGCCGCTGCA CTGCGCCTCC 
ATGTTCGATC ATCCGGACAT TGTGTCCTAT CTGGTGGCCG AGGGAGCGGA CATCAATGCC 
CTGGACAAGG AGCATCGCTC TCCGTTACTG CTGGCGGCAT CTCGTAGCGG TTGGAAAACA 
GTCCACCTCC TGATTCGCCT GGGGGCGTGC ATTAGTGTCA AGGACGCCGC CGCCCGCAAT 
GTGCTGCACT TCGTCATCAT GAACGGCGGC CGGCTGACGG ACTTCGCGGA GCAGGTGGCC 
AATTGCCAGA CGCAGGCGCA GCTGAAGCTG CTGCTCAACG AGAAGGACAG CATGGGCTGC 
TCGCCGCTGC ACTACGCCAG TCGGGATGGA CACATCCGTT CGTTGGAGAA CCTCATTCGA 
CTGGGAGCCT GCATCAACCT GAAGAACAAC AACAACGAGA GTCCGCTGCA CTTTGCCGCA 
CGCTACGGAA GATACAATAC GGTGCGGCAG CTCTTGGATT CCGAGAAGGG ATCCTTCATC 
ATCAACGAAA GTGACGGTGC AGGGATGACA CCACTGCACA TATCCTCGCA GCAAGGACAC 
ACGCGAGTGG TGCAGCTGCT ACTCAATCGA GGAGCCCTGC TCCATCGGGA CCACACCGGA 
CGCAATCCTC TCCAGCTAGC GGCCATGTCC GGATACACCG AGACCATCGA GCTGCTGCAC 
TCGGTGCACT CGCATCTGCT CGATCAGGTG GACAAGGATG GGAACACCGC TCTTCACCTG 
GCCACCATGG AGAATAAGCC CCATGCGATC TCCGTGCTGA TGTCTATGGG CTGTAAGCTG 
GTCTATAACG TTCTGGACAT GAGTGCCATT GACTATGCCA TCTACTACAA ATATCCGGAG 
GCTGCCCTGG CCATGGTCAC CCACGAGGAG CGGGCCAACG AGGTGATGGC TCTGCGTTCC 
GACAAGCATC CGTGCGTGAC CCTCGCCTTA ATTGCCTCCA TGCCCAAGGT ATTCGAGGCG 
GTGCAGGACA AGTGCATTAC CAAGGCCAAT TGCAAGAAGG ACTCGAAGAG TTTCTACATC 
AAATACTCTT TCGCATTCTT GCAATGCCCC TTTATGTTTG CCAAGATTGA TGAGAAAACC 
GGAGAGTCGA TTACGACCGC CAGTCCCATT CCGTTGCCGG CTTTGAATAC CATGGTAACA 
CATGGCAGGG TGGAGCTGCT GGCCCATCCG CTCAGTCAGA AGTATCTGCA GATGAAGTGG 
AACTCCTACG GCAAGTACTT TCACCTGGCC AACCTGCTAA TCTACTCGAT ATTCCTGGTC 
TTTGTAACCA TCTACTCTTC GCTGATGATG AACAACATCG AACTGAAGGC TGGGGACAAC 
AAGACGATGA GTCAATACTG CAATATGGGA TGGGAGCAGC TGACCATGAA TCTCTCGCAG 
AACCCGTCGG TGGCATCACA GATTCGTTTG GATTCCTGCG AGGAGCGTAT AAATAGAACC 
ACTGCAATAC TTTTCTGTGC GGTGGTCATC GTGGTCTATA TACTGCTCAA CTCGATGCGG 
GAACTAATAC AGATATACCA GCAGAAATTG CACTATATCC TGGAGACAGT TAATTTGATA 
TCCTGGGTGC TGTACATCTC GGCTTTGGTG ATGGTAACAC CGGCATTTCA GCCGGATGGA 
GGAATCAATA CCATTCATTA CTCGGCCGCT TCAATAGCAG TCTTTCTGTC GTGGTTCCGA 
TTGCTACTGT TCCTGCAAAG ATTCGACCAG GTCGGCATCT ATGTGGTCAT GTTCTTGGAG 
ATTCTGCAGA CGCTCATTAA AGTGCTGATG GTATTCTCCA TACTTATAAT CGCCTTTGGT 
CTGGCTTTCT ATATACTACT TTCAAAGATT ATTGACCCCC AACCGAACCA CTTGTCCTTC 
TCCAACATAC CCATGTCCTT GCTGCGAACT TTCTCAATGA TGCTGGGCGA GCTGGACTTT 
GTGGGTACCT ATGTGAACAC CTACTATCGG GATCAGTTGA AAGTGCCCAT GACATCCTTT 
TTGATTTTGA GTGTCTTTAT GATCCTTATG CCCATTCTTC TGATGAACTT GCTCATCGGT 
TTGGCCGTCG GCGATATTGA GTCAGTGCGT CGCAATGCCC AGCTCAAGAG ACTGGCCATG 
CAGGTGGTGC TCCACACGGA GCTGGAGAGG AAGTTGCCCC ATGTCTGGCT GCAGCGAGTT 
GACAAGATGG AGCTGATTGA GTATCCCAAT GAAACCAAGT GCAAGCTGGG CTTCTGCGAT 
TTCATCCTGC GCAAGTGGTT CTCGAATCCA TTCACCGAGG ATTCCTCCAT GGACGTCATC 
TCCTTCGACA ACAATGATGA CTACATCAAC GCAGAATTGG AACGGCAGAG GCGAAAGTTG 
CGCGACATAA GTCGCATGCT GGAGCAACAG CACCATCTGG TTCGGCTCAT TGTCCAAAAG 
ATGGAGATCA AGACGGAGGC GGATGACGTG GACGAGGGTA TATCCCCAAA CGAGTTGCGA 
TCCGTCGTCG GTTTGAGATC GGCAGGCGGA AATCGATGGA ACTCGCCGCG AGTCCGGAAT 
AAACTCCGAG CCGCCCTGAG CTTCAATAAG AGCATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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TrpA1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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