Ect4 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ect4
Entrez Gene ID	38895
Full Name	Ectoderm-expressed 4
Synonyms	CG13680,CG13681,CG34373,CG43119,CG7905,CG7915,Dmel\CG43119,Dmel_CG13680,Dmel_CG13681,Dmel_CG34373,Dmel_CG7915,Ect4/SARM,anon-WO0118547.272,dSarm,dsarm,ect4
General protein information	Preferred Names Ectoderm-expressed 4 Names CG43119 gene product from transcript CG43119-RI CG43119-PD CG43119-PE CG43119-PF CG43119-PG CG43119-PH CG43119-PI CG43119-PJ CG43119-PK Ect4-PD Ect4-PE Ect4-PF Ect4-PG Ect4-PH Ect4-PI Ect4-PJ Ect4-PK drosophila sterile alpha and armadillo motif
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 66B4-66B5

mRNA and Protein(s)

mRNA	Protein	Name
NM_168254.2	NP_729327.1	Ectoderm-expressed 4, isoform E
NM_001259733.1	NP_001246662.1	Ectoderm-expressed 4, isoform I
NM_001274627.1	NP_001261556.1	Ectoderm-expressed 4, isoform K
NM_001043129.3	NP_001036594.1	Ectoderm-expressed 4, isoform F
NM_001104075.2	NP_001097545.1	Ectoderm-expressed 4, isoform H
NM_001259734.1	NP_001246663.1	Ectoderm-expressed 4, isoform J
NM_001104071.2	NP_001097541.1	Ectoderm-expressed 4, isoform D
NM_001104074.3	NP_001097544.1	Ectoderm-expressed 4, isoform G

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	sarm1	NP_001124068.1
Drosophila melanogaster (fruit fly)	Ect4	NP_001246663.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005901	XP_315931.4
Xenopus tropicalis (tropical clawed frog)	sarm1	XP_002937189.2
Pan troglodytes (chimpanzee)	SARM1	XP_003315585.1
Homo sapiens (human)	SARM1	NP_055892.2
	SARM1	XP_001106275.2
Rattus norvegicus (Norway rat)	Sarm1	NP_001099287.1
Caenorhabditis elegans (roundworm)	tir-1	NP_497784.1
Canis lupus familiaris (dog)	SARM1	XP_548285.2
Mus musculus (house mouse)	Sarm1	NP_001161993.1
Gallus gallus (chicken)	SARM1	XP_415814.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In Ect4 knockdown flies Drosophila C virus-induced mortality occurred more quickly and virus accumulation was increased.
Title: Drosophila miR-956 suppression modulates Ectoderm-expressed 4 and inhibits viral replication.

findings show that loss of dSarm (sterile alpha/Armadillo/Toll-Interleukin receptor homology domain protein) cell-autonomously suppresses Wallerian degeneration for weeks after axotomy; results provide direct evidence that axons actively promote their own destruction after injury
Title: dSarm/Sarm1 is required for activation of an injury-induced axon death pathway.

The following Ect4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ect4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa17863	NM_168254.2 Latest version!	Drosophila melanogaster Ectoderm-expressed 4 (Ect4), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa20691	NM_001259734.1 Latest version!	Drosophila melanogaster Ectoderm-expressed 4 (Ect4), transcript variant J, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa17859	NM_001104071.2 Latest version!	Drosophila melanogaster Ectoderm-expressed 4 (Ect4), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa17860	NM_001104074.3 Latest version!	Drosophila melanogaster Ectoderm-expressed 4 (Ect4), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa17861	NM_001104075.2 Latest version!	Drosophila melanogaster Ectoderm-expressed 4 (Ect4), transcript variant H, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa17862	NM_001043129.3 Latest version!	Drosophila melanogaster Ectoderm-expressed 4 (Ect4), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa20689	NM_001259733.1 Latest version!	Drosophila melanogaster Ectoderm-expressed 4 (Ect4), transcript variant I, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa20690	NM_001274627.1 Latest version!	Drosophila melanogaster Ectoderm-expressed 4 (Ect4), transcript variant K, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ect4 ( NM_168254.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729327.1 Drosophila melanogaster Ectoderm-expressed 4 (Ect4), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa17863
Clone ID Related Accession (Same CDS sequence)	NM_168254.2
Accession Version	NM_168254.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ectoderm-expressed 4, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On May 8, 2012 this sequence version replaced NM_168254.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAATC AGGCACCCTG GCCCGTTCGC AAGGGCATCT TCCGCTCGAG CGGTCAGTCG 
GATTTCACGC CAACCCGATC GCCTAGTCCC ATTGTGGAGA TGCCATTATC CCCGCCGCCG 
GTGGCCACGC CCTCGAATAG ATTTGGAATC AATTCACCGC TCTCCCCACC GCCGCAGCCT 
ATTCAAGTGG TGGCGGGTTC AACGACAGCC ACTACCATGT CCACTGCGTC CGCTGCCAGA 
GTGTCTGGTG CAGCTGCCTC CACTTCATCC TCGTCCTCCT CTTCTTCGTC GTCGCAGTGC 
TCGTCGCAAA CCTCGTCGAG TTCTAGTTCG CACACCAGAG TACGTAAAAG TTCTAACCCG 
CCCCCACAGC CAATTACCAG TTGTCCACTG TCGCCACCAC CACCACCACC ACCACAGCAA 
CAACAACAAC TACCACAACA ACTGCCACCT CCAACGCCCA TCAACAACAC CAACCACTCA 
GCAATTACAA CACCCAATCA CAACAATAAC CACAACTGCA ATCAAATGAG AAATGTACGC 
GAAATTCCCA TTGAGGTGGA GCAGAGCAAG GAAGCCCTCT CACTCCGCAC CGGCGACATC 
ACACAGCAGG CGAGCAACAA TGTGGCGGCC TCCAGTATTA CGGTGCAAAG TGAGAATTTC 
TCCGCGGACA AGAAGGCGAT ATCCCAGTCG CAGCAATCGC AGACGATGAC CTCCAACGGG 
ATCATCAGTC AGGAGAAACA TGTATCCTCC GCCTCGCAGG CCAACTACTC GATGTCGCAC 
AAGGGCGTTT CCAGTACCGG CAGTAGCATG ATCACCAGCT CCTCGCAAAT GTCCGCGATG 
AATGGCCAGA TGCTGAAGCT CGCCGATCTC AAGTTGGATG ACCTCAAGTC GCTGACAGCT 
GGCAGTGGGC AGCAGGAGAT CGAGCAGACC ATCAACAAGT ATTCCAATAT GCTGACCTCA 
ATAGTGAGCT CCCTGCAAGA AGATGAGCGA GGTGGTAGTG CCATAACCGT CCACGATGTT 
GGTGGCAAGA AGTCTCAGTA CCTGGAGAAG ATCAACGAAG TCATTCGTCG AGCTTGGGCA 
GTGCCCACAC ATGGCCACGA GCTGGGCTAC TCATTGTGCA ATTCCCTGCG CCAAAGTGGC 
GGCCTCGATC TTCTCATGAA GAACTGCGTT AAGCCCGATC TCCAGTTTTC CTCCGCACAG 
CTTCTTGAGC AGTGCCTAAC CACCGAGAAC CGCAAACATG TGGTGGATAA TGGTCTGGAT 
AAGGTGGTCA ATGTGGCCTG TGTCTGCACA AAGAATTCCA ATATGGAGCA CTCCCGCGTG 
GGCACTGGCA TCCTGGAGCA TCTGTTCAAA CACTCCGAGG GCACCTGTTC GGATGTGATA 
AGGCTAGGTG GTCTGGATGC CGTGCTCTTT GAGTGCCGCA CCAGTGATTT GGAAACTCTG 
CGACACTGCG CTAGTGCACT GGCAAATTTA TCTCTATATG GTGGAGCCGA GAATCAGGAG 
GAGATGATCC TACGCAAGGT GCCCATGTGG TTGTTCCCAT TGGCCTTCCA CAACGATGAT 
AACATCAAGT ACTATGCCTG CTTGGCCATT GCAGTGCTGG TAGCCAACAA GGAGATTGAA 
GCCGAGGTGC TGAAGTCTGG ATGCTTGGAT TTGGTGGAAC CCTTTGTCAC CTCCCACGAC 
CCCTCGGCTT TTGCGAGATC CAATCTGGCA CATGCCCACG GGCAAAGCAA ACATTGGCTG 
AAAAGATTGG TTCCAGTTTT GAGTTCCAAT CGCGAAGAGG CCCGCAATCT GGCTGCCTTC 
CATTTTTGCA TGGAGGCGGG CATCAAGAGG GAGCAGGGTA ACACCGACAT CTTCCGCGAG 
ATCAACGCTA TTGAAGCTTT GAAAAATGTG GCCAGCTGTC CAAATGCCAT TGCATCCAAG 
TTCGCCGCTC AGGCTCTGAG ATTGATTGGA GAGACAGTTC CGCACAAGCT GTCCCAACAG 
GTTCCCTTGT GGTCCGTGGA GGATGTGCAG GAGTGGGTAA AGCAAATCGG TTTCAATGAC 
TACATCGACA AGTTCAACGA GTCCCAGGTG GACGGGGATC TTTTACTGAA GCTCAATCAG 
GATAACCTCC GGGCTGATAT TGGAATCGGT AATGGAATAC TTCTAAAGCG TTTTGAACGC 
GAGCTGCAAA ATCTCAAGCG TATGGCTGAC TACTCATCCA AGGACACGGC CAAGATGCAC 
CAATTTCTCT CGGAGATCGG TACTGATTAC TGCACCTACA CGTATGCCAT GCTGAATGCT 
GGAATTGACA AGTGTGCACT GCCGCATGTC AATGAGGATA TGCTGATGAC AGAGTGTGGC 
ATCCACAACT CGATCCATCG TCTGAGAATT CTCAATGCGG TCAAAAACTT GGAGAATTCG 
CTGCCCAGCT CGTCGGAGGA GAACATGGCC AAGACGCTAG ATGTTTTCGT TAGCTATAGG 
CGATCAAACG GCTCCCAACT AGCCAGTCTT CTCAAGGTGC ATCTGCAGCT TCGTGGTTTC 
TCCGTTTTCA TCGACGTGGA GCGCCTGGAG GCGGGCAAAT TTGACAACGG CCTATTGAAC 
AGTATTCGAC AGGCAAAGAA CTTTGTCTTA GTATTAACTC CCGATGCCCT GCACCGCTGC 
ATTAACGACG AGGACTGCAA GGACTGGGTG CATCGCGAAA TCGTGGCTGC CCTGAACTCG 
AACTGCAACA TTATACCCAT CATAGACCAG CAATTCGATT GGCCCGAGGT GGAGCGACTT 
CCCGAGGACA TGCGCAGTGT GGCCCATTTT AACGGTGTTA ATTGGATCCA CGACTACCAG 
GACGCATGCA TCGATAAGCT CGAAAGATTT TTGCGCGGCG AAAAGAATAT CGATCGCATT 
GCGGCGATGG TGCCCGGCAC CCCCGGTTCG GTCTCATACC AAAGAATGCA CAGCAACGAC 
TCCGACTACC AGAGCGGAGG AGCAGGCGCC GGATCAGGAG CAGGAACTGG AGGAGGAGGT 
GGTGGCGGCG TTACGGGCAG TGTGGTGGAT GGCCTGATGG TGGCGGCCAA TGGCAGCGGA 
CAAGGTGGGC CAACGAGTAC TACATCTACT ACTTCTTCTA CTCCCAACTC AAATTCAAGC 
TCAAGCTCTA ACCAGAGTCC CCCAGCGGCT CCAGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729327.1
CDS	354..3512
Translation	MSNQAPWPVRKGIFRSSGQSDFTPTRSPSPIVEMPLSPPPVATPSNRFGINSPLSPPPQPIQVVAGSTTATTMSTASAARVSGAAASTSSSSSSSSSSQCSSQTSSSSSSHTRVRKSSNPPPQPITSCPLSPPPPPPPQQQQQLPQQLPPPTPINNTNHSAITTPNHNNNHNCNQMRNVREIPIEVEQSKEALSLRTGDITQQASNNVAASSITVQSENFSADKKAISQSQQSQTMTSNGIISQEKHVSSASQANYSMSHKGVSSTGSSMITSSSQMSAMNGQMLKLADLKLDDLKSLTAGSGQQEIEQTINKYSNMLTSIVSSLQEDERGGSAITVHDVGGKKSQYLEKINEVIRRAWAVPTHGHELGYSLCNSLRQSGGLDLLMKNCVKPDLQFSSAQLLEQCLTTENRKHVVDNGLDKVVNVACVCTKNSNMEHSRVGTGILEHLFKHSEGTCSDVIRLGGLDAVLFECRTSDLETLRHCASALANLSLYGGAENQEEMILRKVPMWLFPLAFHNDDNIKYYACLAIAVLVANKEIEAEVLKSGCLDLVEPFVTSHDPSAFARSNLAHAHGQSKHWLKRLVPVLSSNREEARNLAAFHFCMEAGIKREQGNTDIFREINAIEALKNVASCPNAIASKFAAQALRLIGETVPHKLSQQVPLWSVEDVQEWVKQIGFNDYIDKFNESQVDGDLLLKLNQDNLRADIGIGNGILLKRFERELQNLKRMADYSSKDTAKMHQFLSEIGTDYCTYTYAMLNAGIDKCALPHVNEDMLMTECGIHNSIHRLRILNAVKNLENSLPSSSEENMAKTLDVFVSYRRSNGSQLASLLKVHLQLRGFSVFIDVERLEAGKFDNGLLNSIRQAKNFVLVLTPDALHRCINDEDCKDWVHREIVAALNSNCNIIPIIDQQFDWPEVERLPEDMRSVAHFNGVNWIHDYQDACIDKLERFLRGEKNIDRIAAMVPGTPGSVSYQRMHSNDSDYQSGGAGAGSGAGTGGGGGGGVTGSVVDGLMVAANGSGQGGPTSTTSTTSSTPNSNSSSSSNQSPPAAPA

Target ORF information:

RefSeq Version	NM_168254.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ectoderm-expressed 4 (Ect4), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168254.2

ORF Insert Sequence:

ATGTCCAATC AGGCACCCTG GCCCGTTCGC AAGGGCATCT TCCGCTCGAG CGGTCAGTCG 
GATTTCACGC CAACCCGATC GCCTAGTCCC ATTGTGGAGA TGCCATTATC CCCGCCGCCG 
GTGGCCACGC CCTCGAATAG ATTTGGAATC AATTCACCGC TCTCCCCACC GCCGCAGCCT 
ATTCAAGTGG TGGCGGGTTC AACGACAGCC ACTACCATGT CCACTGCGTC CGCTGCCAGA 
GTGTCTGGTG CAGCTGCCTC CACTTCATCC TCGTCCTCCT CTTCTTCGTC GTCGCAGTGC 
TCGTCGCAAA CCTCGTCGAG TTCTAGTTCG CACACCAGAG TACGTAAAAG TTCTAACCCG 
CCCCCACAGC CAATTACCAG TTGTCCACTG TCGCCACCAC CACCACCACC ACCACAGCAA 
CAACAACAAC TACCACAACA ACTGCCACCT CCAACGCCCA TCAACAACAC CAACCACTCA 
GCAATTACAA CACCCAATCA CAACAATAAC CACAACTGCA ATCAAATGAG AAATGTACGC 
GAAATTCCCA TTGAGGTGGA GCAGAGCAAG GAAGCCCTCT CACTCCGCAC CGGCGACATC 
ACACAGCAGG CGAGCAACAA TGTGGCGGCC TCCAGTATTA CGGTGCAAAG TGAGAATTTC 
TCCGCGGACA AGAAGGCGAT ATCCCAGTCG CAGCAATCGC AGACGATGAC CTCCAACGGG 
ATCATCAGTC AGGAGAAACA TGTATCCTCC GCCTCGCAGG CCAACTACTC GATGTCGCAC 
AAGGGCGTTT CCAGTACCGG CAGTAGCATG ATCACCAGCT CCTCGCAAAT GTCCGCGATG 
AATGGCCAGA TGCTGAAGCT CGCCGATCTC AAGTTGGATG ACCTCAAGTC GCTGACAGCT 
GGCAGTGGGC AGCAGGAGAT CGAGCAGACC ATCAACAAGT ATTCCAATAT GCTGACCTCA 
ATAGTGAGCT CCCTGCAAGA AGATGAGCGA GGTGGTAGTG CCATAACCGT CCACGATGTT 
GGTGGCAAGA AGTCTCAGTA CCTGGAGAAG ATCAACGAAG TCATTCGTCG AGCTTGGGCA 
GTGCCCACAC ATGGCCACGA GCTGGGCTAC TCATTGTGCA ATTCCCTGCG CCAAAGTGGC 
GGCCTCGATC TTCTCATGAA GAACTGCGTT AAGCCCGATC TCCAGTTTTC CTCCGCACAG 
CTTCTTGAGC AGTGCCTAAC CACCGAGAAC CGCAAACATG TGGTGGATAA TGGTCTGGAT 
AAGGTGGTCA ATGTGGCCTG TGTCTGCACA AAGAATTCCA ATATGGAGCA CTCCCGCGTG 
GGCACTGGCA TCCTGGAGCA TCTGTTCAAA CACTCCGAGG GCACCTGTTC GGATGTGATA 
AGGCTAGGTG GTCTGGATGC CGTGCTCTTT GAGTGCCGCA CCAGTGATTT GGAAACTCTG 
CGACACTGCG CTAGTGCACT GGCAAATTTA TCTCTATATG GTGGAGCCGA GAATCAGGAG 
GAGATGATCC TACGCAAGGT GCCCATGTGG TTGTTCCCAT TGGCCTTCCA CAACGATGAT 
AACATCAAGT ACTATGCCTG CTTGGCCATT GCAGTGCTGG TAGCCAACAA GGAGATTGAA 
GCCGAGGTGC TGAAGTCTGG ATGCTTGGAT TTGGTGGAAC CCTTTGTCAC CTCCCACGAC 
CCCTCGGCTT TTGCGAGATC CAATCTGGCA CATGCCCACG GGCAAAGCAA ACATTGGCTG 
AAAAGATTGG TTCCAGTTTT GAGTTCCAAT CGCGAAGAGG CCCGCAATCT GGCTGCCTTC 
CATTTTTGCA TGGAGGCGGG CATCAAGAGG GAGCAGGGTA ACACCGACAT CTTCCGCGAG 
ATCAACGCTA TTGAAGCTTT GAAAAATGTG GCCAGCTGTC CAAATGCCAT TGCATCCAAG 
TTCGCCGCTC AGGCTCTGAG ATTGATTGGA GAGACAGTTC CGCACAAGCT GTCCCAACAG 
GTTCCCTTGT GGTCCGTGGA GGATGTGCAG GAGTGGGTAA AGCAAATCGG TTTCAATGAC 
TACATCGACA AGTTCAACGA GTCCCAGGTG GACGGGGATC TTTTACTGAA GCTCAATCAG 
GATAACCTCC GGGCTGATAT TGGAATCGGT AATGGAATAC TTCTAAAGCG TTTTGAACGC 
GAGCTGCAAA ATCTCAAGCG TATGGCTGAC TACTCATCCA AGGACACGGC CAAGATGCAC 
CAATTTCTCT CGGAGATCGG TACTGATTAC TGCACCTACA CGTATGCCAT GCTGAATGCT 
GGAATTGACA AGTGTGCACT GCCGCATGTC AATGAGGATA TGCTGATGAC AGAGTGTGGC 
ATCCACAACT CGATCCATCG TCTGAGAATT CTCAATGCGG TCAAAAACTT GGAGAATTCG 
CTGCCCAGCT CGTCGGAGGA GAACATGGCC AAGACGCTAG ATGTTTTCGT TAGCTATAGG 
CGATCAAACG GCTCCCAACT AGCCAGTCTT CTCAAGGTGC ATCTGCAGCT TCGTGGTTTC 
TCCGTTTTCA TCGACGTGGA GCGCCTGGAG GCGGGCAAAT TTGACAACGG CCTATTGAAC 
AGTATTCGAC AGGCAAAGAA CTTTGTCTTA GTATTAACTC CCGATGCCCT GCACCGCTGC 
ATTAACGACG AGGACTGCAA GGACTGGGTG CATCGCGAAA TCGTGGCTGC CCTGAACTCG 
AACTGCAACA TTATACCCAT CATAGACCAG CAATTCGATT GGCCCGAGGT GGAGCGACTT 
CCCGAGGACA TGCGCAGTGT GGCCCATTTT AACGGTGTTA ATTGGATCCA CGACTACCAG 
GACGCATGCA TCGATAAGCT CGAAAGATTT TTGCGCGGCG AAAAGAATAT CGATCGCATT 
GCGGCGATGG TGCCCGGCAC CCCCGGTTCG GTCTCATACC AAAGAATGCA CAGCAACGAC 
TCCGACTACC AGAGCGGAGG AGCAGGCGCC GGATCAGGAG CAGGAACTGG AGGAGGAGGT 
GGTGGCGGCG TTACGGGCAG TGTGGTGGAT GGCCTGATGG TGGCGGCCAA TGGCAGCGGA 
CAAGGTGGGC CAACGAGTAC TACATCTACT ACTTCTTCTA CTCCCAACTC AAATTCAAGC 
TCAAGCTCTA ACCAGAGTCC CCCAGCGGCT CCAGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ect4 ( NM_001259734.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246663.1 Drosophila melanogaster Ectoderm-expressed 4 (Ect4), transcript variant J, mRNA.

Price & Availability↑

CloneID	OFa20691
Clone ID Related Accession (Same CDS sequence)	NM_001259734.1
Accession Version	NM_001259734.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3180bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ectoderm-expressed 4, isoform J
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGGCGG CGGAAATCAA GCGAGATCTC ACAAACATCC AGAAGTCCAT GTCGGAAATC 
AACGATCTGG CCAAGGAGCG GATCACCGGC GGACCTGGAT CCATTAGCAC CACCTCAGCC 
TCGGCGATCA CAGCACCCAG CACGATGTCC CAGACGACGA CGAGCCGGCT GGCACCCAAA 
CTCACATCCG CCCATCCGTC AATCGACGAT CTCAGGGGTC TCAGTAGGCA GGACAAAATC 
ACCCAGCTGC AAAAAAAAAT TCGCGCCTCG TTCGAGAACC TGGTGGACCA CGATGACAGC 
AATGTGATCG TGACCCTGCC GGATGACGAC GACTGCCCGC ATAACCATTT TGGCAGCGGC 
CTCAACCTTA CCCATCCCAC CGCCGCCCAG CTGAGTGCTA GTGGGCTGAG CGGGTCGAGT 
AAGACAATCG ACACGATTAA GTTCCAGGAG AAGAGCATGA AGACCGAGTC CAAGACCAAG 
GTGGTCACCG ATGGCTTCAG TTCCGAGCAG GCCACTAGCA ATTCGGCGGA GATGAAACGT 
TTGCAGGCCG GAGATATAGA CTATCAAGAA AGCAAGGGTG CGTCGGCGAT GAGAAATCGA 
TTGGAGGTGG ATGGGGTCAA AACGGAGGAG AATGCAGCCG TCATTAAGGA AGCCCTCTCA 
CTCCGCACCG GCGACATCAC ACAGCAGGCG AGCAACAATG TGGCGGCCTC CAGTATTACG 
GTGCAAAGTG AGAATTTCTC CGCGGACAAG AAGGCGATAT CCCAGTCGCA GCAATCGCAG 
ACGATGACCT CCAACGGGAT CATCAGTCAG GAGAAACATG TATCCTCCGC CTCGCAGGCC 
AACTACTCGA TGTCGCACAA GGGCGTTTCC AGTACCGGCA GTAGCATGAT CACCAGCTCC 
TCGCAAATGT CCGCGATGAA TGGCCAGATG CTGAAGCTCG CCGATCTCAA GTTGGATGAC 
CTCAAGTCGC TGACAGCTGG CAGTGGGCAG CAGGAGATCG AGCAGACCAT CAACAAGTAT 
TCCAATATGC TGACCTCAAT AGTGAGCTCC CTGCAAGAAG ATGAGCGAGG TGGTAGTGCC 
ATAACCGTCC ACGATGTTGG TGGCAAGAAG TCTCAGTACC TGGAGAAGAT CAACGAAGTC 
ATTCGTCGAG CTTGGGCAGT GCCCACACAT GGCCACGAGC TGGGCTACTC ATTGTGCAAT 
TCCCTGCGCC AAAGTGGCGG CCTCGATCTT CTCATGAAGA ACTGCGTTAA GCCCGATCTC 
CAGTTTTCCT CCGCACAGCT TCTTGAGCAG TGCCTAACCA CCGAGAACCG CAAACATGTG 
GTGGATAATG GTCTGGATAA GGTGGTCAAT GTGGCCTGTG TCTGCACAAA GAATTCCAAT 
ATGGAGCACT CCCGCGTGGG CACTGGCATC CTGGAGCATC TGTTCAAACA CTCCGAGGGC 
ACCTGTTCGG ATGTGATAAG GCTAGGTGGT CTGGATGCCG TGCTCTTTGA GTGCCGCACC 
AGTGATTTGG AAACTCTGCG ACACTGCGCT AGTGCACTGG CAAATTTATC TCTATATGGT 
GGAGCCGAGA ATCAGGAGGA GATGATCCTA CGCAAGGTGC CCATGTGGTT GTTCCCATTG 
GCCTTCCACA ACGATGATAA CATCAAGTAC TATGCCTGCT TGGCCATTGC AGTGCTGGTA 
GCCAACAAGG AGATTGAAGC CGAGGTGCTG AAGTCTGGAT GCTTGGATTT GGTGGAACCC 
TTTGTCACCT CCCACGACCC CTCGGCTTTT GCGAGATCCA ATCTGGCACA TGCCCACGGG 
CAAAGCAAAC ATTGGCTGAA AAGATTGGTT CCAGTTTTGA GTTCCAATCG CGAAGAGGCC 
CGCAATCTGG CTGCCTTCCA TTTTTGCATG GAGGCGGGCA TCAAGAGGGA GCAGGGTAAC 
ACCGACATCT TCCGCGAGAT CAACGCTATT GAAGCTTTGA AAAATGTGGC CAGCTGTCCA 
AATGCCATTG CATCCAAGTT CGCCGCTCAG GCTCTGAGAT TGATTGGAGA GACAGTTCCG 
CACAAGCTGT CCCAACAGGT TCCCTTGTGG TCCGTGGAGG ATGTGCAGGA GTGGGTAAAG 
CAAATCGGTT TCAATGACTA CATCGACAAG TTCAACGAGT CCCAGGTGGA CGGGGATCTT 
TTACTGAAGC TCAATCAGGA TAACCTCCGG GCTGATATTG GAATCGGTAA TGGAATACTT 
CTAAAGCGTT TTGAACGCGA GCTGCAAAAT CTCAAGCGTA TGGCTGACTA CTCATCCAAG 
GACACGGCCA AGATGCACCA ATTTCTCTCG GAGATCGGTA CTGATTACTG CACCTACACG 
TATGCCATGC TGAATGCTGG AATTGACAAG TGTGCACTGC CGCATGTCAA TGAGGATATG 
CTGATGACAG AGTGTGGCAT CCACAACTCG ATCCATCGTC TGAGAATTCT CAATGCGGTC 
AAAAACTTGG AGAATTCGCT GCCCAGCTCG TCGGAGGAGA ACATGGCCAA GACGCTAGAT 
GTTTTCGTTA GCTATAGGCG ATCAAACGGC TCCCAACTAG CCAGTCTTCT CAAGGTGCAT 
CTGCAGCTTC GTGGTTTCTC CGTTTTCATC GACGTGGAGC GCCTGGAGGC GGGCAAATTT 
GACAACGGCC TATTGAACAG TATTCGACAG GCAAAGAACT TTGTCTTAGT ATTAACTCCC 
GATGCCCTGC ACCGCTGCAT TAACGACGAG GACTGCAAGG ACTGGGTGCA TCGCGAAATC 
GTGGCTGCCC TGAACTCGAA CTGCAACATT ATACCCATCA TAGACCAGCA ATTCGATTGG 
CCCGAGGTGG AGCGACTTCC CGAGGACATG CGCAGTGTGG CCCATTTTAA CGGTGTTAAT 
TGGATCCACG ACTACCAGGA CGCATGCATC GATAAGCTCG AAAGATTTTT GCGCGGCGAA 
AAGAATATCG ATCGCATTGC GGCGATGGTG CCCGGCACCC CCGGTTCGGT CTCATACCAA 
AGAATGCACA GCAACGACTC CGACTACCAG AGCGGAGGAG CAGGCGCCGG ATCAGGAGCA 
GGAACTGGAG GAGGAGGTGG TGGCGGCGTT ACGGGCAGTG TGGTGGATGG CCTGATGGTG 
GCGGCCAATG GCAGCGGACA AGACCATAAA AGCGAAATTG GCTACCGCAA TCCGTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246663.1
CDS	475..3654
Translation	MKAAEIKRDLTNIQKSMSEINDLAKERITGGPGSISTTSASAITAPSTMSQTTTSRLAPKLTSAHPSIDDLRGLSRQDKITQLQKKIRASFENLVDHDDSNVIVTLPDDDDCPHNHFGSGLNLTHPTAAQLSASGLSGSSKTIDTIKFQEKSMKTESKTKVVTDGFSSEQATSNSAEMKRLQAGDIDYQESKGASAMRNRLEVDGVKTEENAAVIKEALSLRTGDITQQASNNVAASSITVQSENFSADKKAISQSQQSQTMTSNGIISQEKHVSSASQANYSMSHKGVSSTGSSMITSSSQMSAMNGQMLKLADLKLDDLKSLTAGSGQQEIEQTINKYSNMLTSIVSSLQEDERGGSAITVHDVGGKKSQYLEKINEVIRRAWAVPTHGHELGYSLCNSLRQSGGLDLLMKNCVKPDLQFSSAQLLEQCLTTENRKHVVDNGLDKVVNVACVCTKNSNMEHSRVGTGILEHLFKHSEGTCSDVIRLGGLDAVLFECRTSDLETLRHCASALANLSLYGGAENQEEMILRKVPMWLFPLAFHNDDNIKYYACLAIAVLVANKEIEAEVLKSGCLDLVEPFVTSHDPSAFARSNLAHAHGQSKHWLKRLVPVLSSNREEARNLAAFHFCMEAGIKREQGNTDIFREINAIEALKNVASCPNAIASKFAAQALRLIGETVPHKLSQQVPLWSVEDVQEWVKQIGFNDYIDKFNESQVDGDLLLKLNQDNLRADIGIGNGILLKRFERELQNLKRMADYSSKDTAKMHQFLSEIGTDYCTYTYAMLNAGIDKCALPHVNEDMLMTECGIHNSIHRLRILNAVKNLENSLPSSSEENMAKTLDVFVSYRRSNGSQLASLLKVHLQLRGFSVFIDVERLEAGKFDNGLLNSIRQAKNFVLVLTPDALHRCINDEDCKDWVHREIVAALNSNCNIIPIIDQQFDWPEVERLPEDMRSVAHFNGVNWIHDYQDACIDKLERFLRGEKNIDRIAAMVPGTPGSVSYQRMHSNDSDYQSGGAGAGSGAGTGGGGGGGVTGSVVDGLMVAANGSGQDHKSEIGYRNPL

Target ORF information:

RefSeq Version	NM_001259734.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ectoderm-expressed 4 (Ect4), transcript variant J, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259734.1

ORF Insert Sequence:

ATGAAGGCGG CGGAAATCAA GCGAGATCTC ACAAACATCC AGAAGTCCAT GTCGGAAATC 
AACGATCTGG CCAAGGAGCG GATCACCGGC GGACCTGGAT CCATTAGCAC CACCTCAGCC 
TCGGCGATCA CAGCACCCAG CACGATGTCC CAGACGACGA CGAGCCGGCT GGCACCCAAA 
CTCACATCCG CCCATCCGTC AATCGACGAT CTCAGGGGTC TCAGTAGGCA GGACAAAATC 
ACCCAGCTGC AAAAAAAAAT TCGCGCCTCG TTCGAGAACC TGGTGGACCA CGATGACAGC 
AATGTGATCG TGACCCTGCC GGATGACGAC GACTGCCCGC ATAACCATTT TGGCAGCGGC 
CTCAACCTTA CCCATCCCAC CGCCGCCCAG CTGAGTGCTA GTGGGCTGAG CGGGTCGAGT 
AAGACAATCG ACACGATTAA GTTCCAGGAG AAGAGCATGA AGACCGAGTC CAAGACCAAG 
GTGGTCACCG ATGGCTTCAG TTCCGAGCAG GCCACTAGCA ATTCGGCGGA GATGAAACGT 
TTGCAGGCCG GAGATATAGA CTATCAAGAA AGCAAGGGTG CGTCGGCGAT GAGAAATCGA 
TTGGAGGTGG ATGGGGTCAA AACGGAGGAG AATGCAGCCG TCATTAAGGA AGCCCTCTCA 
CTCCGCACCG GCGACATCAC ACAGCAGGCG AGCAACAATG TGGCGGCCTC CAGTATTACG 
GTGCAAAGTG AGAATTTCTC CGCGGACAAG AAGGCGATAT CCCAGTCGCA GCAATCGCAG 
ACGATGACCT CCAACGGGAT CATCAGTCAG GAGAAACATG TATCCTCCGC CTCGCAGGCC 
AACTACTCGA TGTCGCACAA GGGCGTTTCC AGTACCGGCA GTAGCATGAT CACCAGCTCC 
TCGCAAATGT CCGCGATGAA TGGCCAGATG CTGAAGCTCG CCGATCTCAA GTTGGATGAC 
CTCAAGTCGC TGACAGCTGG CAGTGGGCAG CAGGAGATCG AGCAGACCAT CAACAAGTAT 
TCCAATATGC TGACCTCAAT AGTGAGCTCC CTGCAAGAAG ATGAGCGAGG TGGTAGTGCC 
ATAACCGTCC ACGATGTTGG TGGCAAGAAG TCTCAGTACC TGGAGAAGAT CAACGAAGTC 
ATTCGTCGAG CTTGGGCAGT GCCCACACAT GGCCACGAGC TGGGCTACTC ATTGTGCAAT 
TCCCTGCGCC AAAGTGGCGG CCTCGATCTT CTCATGAAGA ACTGCGTTAA GCCCGATCTC 
CAGTTTTCCT CCGCACAGCT TCTTGAGCAG TGCCTAACCA CCGAGAACCG CAAACATGTG 
GTGGATAATG GTCTGGATAA GGTGGTCAAT GTGGCCTGTG TCTGCACAAA GAATTCCAAT 
ATGGAGCACT CCCGCGTGGG CACTGGCATC CTGGAGCATC TGTTCAAACA CTCCGAGGGC 
ACCTGTTCGG ATGTGATAAG GCTAGGTGGT CTGGATGCCG TGCTCTTTGA GTGCCGCACC 
AGTGATTTGG AAACTCTGCG ACACTGCGCT AGTGCACTGG CAAATTTATC TCTATATGGT 
GGAGCCGAGA ATCAGGAGGA GATGATCCTA CGCAAGGTGC CCATGTGGTT GTTCCCATTG 
GCCTTCCACA ACGATGATAA CATCAAGTAC TATGCCTGCT TGGCCATTGC AGTGCTGGTA 
GCCAACAAGG AGATTGAAGC CGAGGTGCTG AAGTCTGGAT GCTTGGATTT GGTGGAACCC 
TTTGTCACCT CCCACGACCC CTCGGCTTTT GCGAGATCCA ATCTGGCACA TGCCCACGGG 
CAAAGCAAAC ATTGGCTGAA AAGATTGGTT CCAGTTTTGA GTTCCAATCG CGAAGAGGCC 
CGCAATCTGG CTGCCTTCCA TTTTTGCATG GAGGCGGGCA TCAAGAGGGA GCAGGGTAAC 
ACCGACATCT TCCGCGAGAT CAACGCTATT GAAGCTTTGA AAAATGTGGC CAGCTGTCCA 
AATGCCATTG CATCCAAGTT CGCCGCTCAG GCTCTGAGAT TGATTGGAGA GACAGTTCCG 
CACAAGCTGT CCCAACAGGT TCCCTTGTGG TCCGTGGAGG ATGTGCAGGA GTGGGTAAAG 
CAAATCGGTT TCAATGACTA CATCGACAAG TTCAACGAGT CCCAGGTGGA CGGGGATCTT 
TTACTGAAGC TCAATCAGGA TAACCTCCGG GCTGATATTG GAATCGGTAA TGGAATACTT 
CTAAAGCGTT TTGAACGCGA GCTGCAAAAT CTCAAGCGTA TGGCTGACTA CTCATCCAAG 
GACACGGCCA AGATGCACCA ATTTCTCTCG GAGATCGGTA CTGATTACTG CACCTACACG 
TATGCCATGC TGAATGCTGG AATTGACAAG TGTGCACTGC CGCATGTCAA TGAGGATATG 
CTGATGACAG AGTGTGGCAT CCACAACTCG ATCCATCGTC TGAGAATTCT CAATGCGGTC 
AAAAACTTGG AGAATTCGCT GCCCAGCTCG TCGGAGGAGA ACATGGCCAA GACGCTAGAT 
GTTTTCGTTA GCTATAGGCG ATCAAACGGC TCCCAACTAG CCAGTCTTCT CAAGGTGCAT 
CTGCAGCTTC GTGGTTTCTC CGTTTTCATC GACGTGGAGC GCCTGGAGGC GGGCAAATTT 
GACAACGGCC TATTGAACAG TATTCGACAG GCAAAGAACT TTGTCTTAGT ATTAACTCCC 
GATGCCCTGC ACCGCTGCAT TAACGACGAG GACTGCAAGG ACTGGGTGCA TCGCGAAATC 
GTGGCTGCCC TGAACTCGAA CTGCAACATT ATACCCATCA TAGACCAGCA ATTCGATTGG 
CCCGAGGTGG AGCGACTTCC CGAGGACATG CGCAGTGTGG CCCATTTTAA CGGTGTTAAT 
TGGATCCACG ACTACCAGGA CGCATGCATC GATAAGCTCG AAAGATTTTT GCGCGGCGAA 
AAGAATATCG ATCGCATTGC GGCGATGGTG CCCGGCACCC CCGGTTCGGT CTCATACCAA 
AGAATGCACA GCAACGACTC CGACTACCAG AGCGGAGGAG CAGGCGCCGG ATCAGGAGCA 
GGAACTGGAG GAGGAGGTGG TGGCGGCGTT ACGGGCAGTG TGGTGGATGG CCTGATGGTG 
GCGGCCAATG GCAGCGGACA AGACCATAAA AGCGAAATTG GCTACCGCAA TCCGTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ect4 ( NM_001104071.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097541.1 Drosophila melanogaster Ectoderm-expressed 4 (Ect4), transcript variant D, mRNA.

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CloneID	OFa17859
Clone ID Related Accession (Same CDS sequence)	NM_001104071.2
Accession Version	NM_001104071.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ectoderm-expressed 4, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On May 8, 2012 this sequence version replaced NM_001104071.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCAATC GTTTGAGCGG CTTGTGGTGC AAGAAAACAA CATTGAGTAT AACCCAACAA 
GAAGAGCAGC AGTATAACCG GGAAATTCGG AACGAAAAGA GCCCACCTCG CAGTGGTCGT 
AAATCGAATC GAATTCGAAC CAAGTCGAGT GGTTCCGGTG ATGGTAGAGG CACTTTTCGT 
CTTGCCAGCA TACGCCGTTT ATCACTGAGA GATAACCCCA AGAAGACGAT TAAAACCCCT 
TTGGATAACC CCAGCATTGC AACCGTTGAA CAAACCTTTC GTGAGCTAAC CAAGCGAGCT 
GATCTCGATT CCAGCATGAA AGTTAATGCA CAACTAATGC CCACTGTCAA GATGACAGAC 
TTCTCGGATA GCTCGCCAAG TGGGGTGCTG AGTCCACCCA CCTCGCTGAT CGAGAATTTC 
CACAAGAAGA ACAGCTCACC GCAGCCACTT GGCAGTAGCA GCGCCATAAG CACCCTAACT 
CCAACAACCT TGAATGGATC TGTGGTTGGC GGTGGAGTGG GTGGTGCTGG ACACCACAGC 
ATCGAGAATG GCAGGCAAAT CAATACCAGC TCTAGTTCAT GCAGTAGTCT TAAGGAAACC 
TCACACCAGT CCACATCCAC ATCACAACAG GTTGTCAACA GCAGCAGCAG CACAACGACG 
CGGGTGGAGA AGAAGTCTCA GCGTTTGCAC CACCACATAA CCTCCTCATC CTCCAGCTCT 
TCAACCACCC AGGCTCTGTC CACCTCATCA GAGATGAAGG CGGCGGAAAT CAAGCGAGAT 
CTCACAAACA TCCAGAAGTC CATGTCGGAA ATCAACGATC TGGCCAAGGA GCGGATCACC 
GGCGGACCTG GATCCATTAG CACCACCTCA GCCTCGGCGA TCACAGCACC CAGCACGATG 
TCCCAGACGA CGACGAGCCG GCTGGCACCC AAACTCACAT CCGCCCATCC GTCAATCGAC 
GATCTCAGGG GTCTCAGTAG GCAGGACAAA ATCACCCAGC TGCAAAAAAA AATTCGCGCC 
TCGTTCGAGA ACCTGGTGGA CCACGATGAC AGCAATGTGA TCGTGACCCT GCCGGATGAC 
GACGACTGCC CGCATAACCA TTTTGGCAGC GGCCTCAACC TTACCCATCC CACCGCCGCC 
CAGCTGAGTG CTAGTGGGCT GAGCGGGTCG AGTAAGACAA TCGACACGAT TAAGTTCCAG 
GAGAAGAGCA TGAAGACCGA GTCCAAGACC AAGGTGGTCA CCGATGGCTT CAGTTCCGAG 
CAGGCCACTA GCAATTCGGC GGAGATGAAA CGTTTGCAGG CCGGAGATAT AGACTATCAA 
GAAAGCAAGG GTGCGTCGGC GATGAGAAAT CGATTGGAGG TGGATGGGGT CAAAACGGAG 
GAGAATGCAG CCGTCATTAA GGAAGCCCTC TCACTCCGCA CCGGCGACAT CACACAGCAG 
GCGAGCAACA ATGTGGCGGC CTCCAGTATT ACGGTGCAAA GTGAGAATTT CTCCGCGGAC 
AAGAAGGCGA TATCCCAGTC GCAGCAATCG CAGACGATGA CCTCCAACGG GATCATCAGT 
CAGGAGAAAC ATGTATCCTC CGCCTCGCAG GCCAACTACT CGATGTCGCA CAAGGGCGTT 
TCCAGTACCG GCAGTAGCAT GATCACCAGC TCCTCGCAAA TGTCCGCGAT GAATGGCCAG 
ATGCTGAAGC TCGCCGATCT CAAGTTGGAT GACCTCAAGT CGCTGACAGC TGGCAGTGGG 
CAGCAGGAGA TCGAGCAGAC CATCAACAAG TATTCCAATA TGCTGACCTC AATAGTGAGC 
TCCCTGCAAG AAGATGAGCG AGGTGGTAGT GCCATAACCG TCCACGATGT TGGTGGCAAG 
AAGTCTCAGT ACCTGGAGAA GATCAACGAA GTCATTCGTC GAGCTTGGGC AGTGCCCACA 
CATGGCCACG AGCTGGGCTA CTCATTGTGC AATTCCCTGC GCCAAAGTGG CGGCCTCGAT 
CTTCTCATGA AGAACTGCGT TAAGCCCGAT CTCCAGTTTT CCTCCGCACA GCTTCTTGAG 
CAGTGCCTAA CCACCGAGAA CCGCAAACAT GTGGTGGATA ATGGTCTGGA TAAGGTGGTC 
AATGTGGCCT GTGTCTGCAC AAAGAATTCC AATATGGAGC ACTCCCGCGT GGGCACTGGC 
ATCCTGGAGC ATCTGTTCAA ACACTCCGAG GGCACCTGTT CGGATGTGAT AAGGCTAGGT 
GGTCTGGATG CCGTGCTCTT TGAGTGCCGC ACCAGTGATT TGGAAACTCT GCGACACTGC 
GCTAGTGCAC TGGCAAATTT ATCTCTATAT GGTGGAGCCG AGAATCAGGA GGAGATGATC 
CTACGCAAGG TGCCCATGTG GTTGTTCCCA TTGGCCTTCC ACAACGATGA TAACATCAAG 
TACTATGCCT GCTTGGCCAT TGCAGTGCTG GTAGCCAACA AGGAGATTGA AGCCGAGGTG 
CTGAAGTCTG GATGCTTGGA TTTGGTGGAA CCCTTTGTCA CCTCCCACGA CCCCTCGGCT 
TTTGCGAGAT CCAATCTGGC ACATGCCCAC GGGCAAAGCA AACATTGGCT GAAAAGATTG 
GTTCCAGTTT TGAGTTCCAA TCGCGAAGAG GCCCGCAATC TGGCTGCCTT CCATTTTTGC 
ATGGAGGCGG GCATCAAGAG GGAGCAGGGT AACACCGACA TCTTCCGCGA GATCAACGCT 
ATTGAAGCTT TGAAAAATGT GGCCAGCTGT CCAAATGCCA TTGCATCCAA GTTCGCCGCT 
CAGGCTCTGA GATTGATTGG AGAGACAGTT CCGCACAAGC TGTCCCAACA GGTTCCCTTG 
TGGTCCGTGG AGGATGTGCA GGAGTGGGTA AAGCAAATCG GTTTCAATGA CTACATCGAC 
AAGTTCAACG AGTCCCAGGT GGACGGGGAT CTTTTACTGA AGCTCAATCA GGATAACCTC 
CGGGCTGATA TTGGAATCGG TAATGGAATA CTTCTAAAGC GTTTTGAACG CGAGCTGCAA 
AATCTCAAGC GTATGGCTGA CTACTCATCC AAGGACACGG CCAAGATGCA CCAATTTCTC 
TCGGAGATCG GTACTGATTA CTGCACCTAC ACGTATGCCA TGCTGAATGC TGGAATTGAC 
AAGTGTGCAC TGCCGCATGT CAATGAGGAT ATGCTGATGA CAGAGTGTGG CATCCACAAC 
TCGATCCATC GTCTGAGAAT TCTCAATGCG GTCAAAAACT TGGAGAATTC GCTGCCCAGC 
TCGTCGGAGG AGAACATGGC CAAGACGCTA GATGTTTTCG TTAGCTATAG GCGATCAAAC 
GGCTCCCAAC TAGCCAGTCT TCTCAAGGTG CATCTGCAGC TTCGTGGTTT CTCCGTTTTC 
ATCGACGTGG AGCGCCTGGA GGCGGGCAAA TTTGACAACG GCCTATTGAA CAGTATTCGA 
CAGGCAAAGA ACTTTGTCTT AGTATTAACT CCCGATGCCC TGCACCGCTG CATTAACGAC 
GAGGACTGCA AGGACTGGGT GCATCGCGAA ATCGTGGCTG CCCTGAACTC GAACTGCAAC 
ATTATACCCA TCATAGACCA GCAATTCGAT TGGCCCGAGG TGGAGCGACT TCCCGAGGAC 
ATGCGCAGTG TGGCCCATTT TAACGGTGTT AATTGGATCC ACGACTACCA GGACGCATGC 
ATCGATAAGC TCGAAAGATT TTTGCGCGGC GAAAAGAATA TCGATCGCAT TGCGGCGATG 
GTGCCCGGCA CCCCCGGTTC GGTCTCATAC CAAAGAATGC ACAGCAACGA CTCCGACTAC 
CAGAGCGGAG GAGCAGGCGC CGGATCAGGA GCAGGAACTG GAGGAGGAGG TGGTGGCGGC 
GTTACGGGCA GTGTGGTGGA TGGCCTGATG GTGGCGGCCA ATGGCAGCGG ACAAGCTAAT 
CACCAGGCAA ATAGGTACCG CCAATCTCCC TCACCGGCCC GCCAAAGGGG CAGCACCTCG 
CAGTTGAGTG GTTATTCTCG GGCGCCCTCG AAACGCTCCC AAATCCTCAC CCCGTATCGC 
ACCCAACAGG CAGCCCTGCT CCACAAAACC GGAGCGGGTT CCGCGTCCAT GCAGAATATG 
ATGCCATTGG CGTATCTGCC TCCGCGGAGG AGTTCCGCCG CGGGATTAGG TCATGGATCT 
GGTTCCGGCA TGGGCAGTGG CTATCGATCG CACAGTGTGG ATGGGCTTCT AGATCAGGCG 
GGCTCAACTC CAGAGCAAAG AATAGCAGCA GCTGCGGCCA AGGTGACAGC GGGTAGTACA 
GCTCTAACGA ACGCCAGTTC CACAAGCACT CTGCAGCCCG AAGAAGAGGT GACGGATGCC 
GCCCTCAATG ACTCGGTGAC CCGGCGTGAC AAGCATACTT TATCCCCACC GGGAAATGTA 
CAGCAGCACA GAAAGTCCCG AAGTCTGGAT CATATTCTCA GCAAACAGAC TTTAGCAGAG 
CTGCTTCCAC CGAGTAGCGA ACTCGCAGAT GGAACGCAAA GTATGCAAAA CCTGGCCATT 
CCAATGACCC CGCAGCCCCA ACGAAGGGAC ACCAGCTCCT CCTCGAAATC CCCCACTCCC 
GAAAGACCTC CGCAACCAGC GATGGAAAGG GTAAGGGAAC GCCAGAGTCC TGAGGGTGTG 
AGTGCGACGG AAAGCGAGCG GGAGGATCAG CCAGAAGAAT GTCTGCGACA TGGCAACCAA 
CAGAGGGCAT CTGCCTCGGT TCATCGGGGA GCCAGTTTGA CCAGCAACAA GACCTCCAAC 
TCCTCACTGG GCTCCAACTT TAGTGCCGGA GGAAACAACA AGACGATATT TAATCGAACG 
ATGAAGAAGG TCCGCTCGCT GATAAAAAAC AACGAAATGG AGGACGAGGA ACTTTCGGGC 
ATCATTCTGT CCAAGGCCAC CTCCCCCAAT GCCGGGCGCA TGATATTTTG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097541.1
CDS	170..5083
Translation	MGNRLSGLWCKKTTLSITQQEEQQYNREIRNEKSPPRSGRKSNRIRTKSSGSGDGRGTFRLASIRRLSLRDNPKKTIKTPLDNPSIATVEQTFRELTKRADLDSSMKVNAQLMPTVKMTDFSDSSPSGVLSPPTSLIENFHKKNSSPQPLGSSSAISTLTPTTLNGSVVGGGVGGAGHHSIENGRQINTSSSSCSSLKETSHQSTSTSQQVVNSSSSTTTRVEKKSQRLHHHITSSSSSSSTTQALSTSSEMKAAEIKRDLTNIQKSMSEINDLAKERITGGPGSISTTSASAITAPSTMSQTTTSRLAPKLTSAHPSIDDLRGLSRQDKITQLQKKIRASFENLVDHDDSNVIVTLPDDDDCPHNHFGSGLNLTHPTAAQLSASGLSGSSKTIDTIKFQEKSMKTESKTKVVTDGFSSEQATSNSAEMKRLQAGDIDYQESKGASAMRNRLEVDGVKTEENAAVIKEALSLRTGDITQQASNNVAASSITVQSENFSADKKAISQSQQSQTMTSNGIISQEKHVSSASQANYSMSHKGVSSTGSSMITSSSQMSAMNGQMLKLADLKLDDLKSLTAGSGQQEIEQTINKYSNMLTSIVSSLQEDERGGSAITVHDVGGKKSQYLEKINEVIRRAWAVPTHGHELGYSLCNSLRQSGGLDLLMKNCVKPDLQFSSAQLLEQCLTTENRKHVVDNGLDKVVNVACVCTKNSNMEHSRVGTGILEHLFKHSEGTCSDVIRLGGLDAVLFECRTSDLETLRHCASALANLSLYGGAENQEEMILRKVPMWLFPLAFHNDDNIKYYACLAIAVLVANKEIEAEVLKSGCLDLVEPFVTSHDPSAFARSNLAHAHGQSKHWLKRLVPVLSSNREEARNLAAFHFCMEAGIKREQGNTDIFREINAIEALKNVASCPNAIASKFAAQALRLIGETVPHKLSQQVPLWSVEDVQEWVKQIGFNDYIDKFNESQVDGDLLLKLNQDNLRADIGIGNGILLKRFERELQNLKRMADYSSKDTAKMHQFLSEIGTDYCTYTYAMLNAGIDKCALPHVNEDMLMTECGIHNSIHRLRILNAVKNLENSLPSSSEENMAKTLDVFVSYRRSNGSQLASLLKVHLQLRGFSVFIDVERLEAGKFDNGLLNSIRQAKNFVLVLTPDALHRCINDEDCKDWVHREIVAALNSNCNIIPIIDQQFDWPEVERLPEDMRSVAHFNGVNWIHDYQDACIDKLERFLRGEKNIDRIAAMVPGTPGSVSYQRMHSNDSDYQSGGAGAGSGAGTGGGGGGGVTGSVVDGLMVAANGSGQANHQANRYRQSPSPARQRGSTSQLSGYSRAPSKRSQILTPYRTQQAALLHKTGAGSASMQNMMPLAYLPPRRSSAAGLGHGSGSGMGSGYRSHSVDGLLDQAGSTPEQRIAAAAAKVTAGSTALTNASSTSTLQPEEEVTDAALNDSVTRRDKHTLSPPGNVQQHRKSRSLDHILSKQTLAELLPPSSELADGTQSMQNLAIPMTPQPQRRDTSSSSKSPTPERPPQPAMERVRERQSPEGVSATESEREDQPEECLRHGNQQRASASVHRGASLTSNKTSNSSLGSNFSAGGNNKTIFNRTMKKVRSLIKNNEMEDEELSGIILSKATSPNAGRMIFW

Target ORF information:

RefSeq Version	NM_001104071.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ectoderm-expressed 4 (Ect4), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001104071.2

ORF Insert Sequence:

ATGGGCAATC GTTTGAGCGG CTTGTGGTGC AAGAAAACAA CATTGAGTAT AACCCAACAA 
GAAGAGCAGC AGTATAACCG GGAAATTCGG AACGAAAAGA GCCCACCTCG CAGTGGTCGT 
AAATCGAATC GAATTCGAAC CAAGTCGAGT GGTTCCGGTG ATGGTAGAGG CACTTTTCGT 
CTTGCCAGCA TACGCCGTTT ATCACTGAGA GATAACCCCA AGAAGACGAT TAAAACCCCT 
TTGGATAACC CCAGCATTGC AACCGTTGAA CAAACCTTTC GTGAGCTAAC CAAGCGAGCT 
GATCTCGATT CCAGCATGAA AGTTAATGCA CAACTAATGC CCACTGTCAA GATGACAGAC 
TTCTCGGATA GCTCGCCAAG TGGGGTGCTG AGTCCACCCA CCTCGCTGAT CGAGAATTTC 
CACAAGAAGA ACAGCTCACC GCAGCCACTT GGCAGTAGCA GCGCCATAAG CACCCTAACT 
CCAACAACCT TGAATGGATC TGTGGTTGGC GGTGGAGTGG GTGGTGCTGG ACACCACAGC 
ATCGAGAATG GCAGGCAAAT CAATACCAGC TCTAGTTCAT GCAGTAGTCT TAAGGAAACC 
TCACACCAGT CCACATCCAC ATCACAACAG GTTGTCAACA GCAGCAGCAG CACAACGACG 
CGGGTGGAGA AGAAGTCTCA GCGTTTGCAC CACCACATAA CCTCCTCATC CTCCAGCTCT 
TCAACCACCC AGGCTCTGTC CACCTCATCA GAGATGAAGG CGGCGGAAAT CAAGCGAGAT 
CTCACAAACA TCCAGAAGTC CATGTCGGAA ATCAACGATC TGGCCAAGGA GCGGATCACC 
GGCGGACCTG GATCCATTAG CACCACCTCA GCCTCGGCGA TCACAGCACC CAGCACGATG 
TCCCAGACGA CGACGAGCCG GCTGGCACCC AAACTCACAT CCGCCCATCC GTCAATCGAC 
GATCTCAGGG GTCTCAGTAG GCAGGACAAA ATCACCCAGC TGCAAAAAAA AATTCGCGCC 
TCGTTCGAGA ACCTGGTGGA CCACGATGAC AGCAATGTGA TCGTGACCCT GCCGGATGAC 
GACGACTGCC CGCATAACCA TTTTGGCAGC GGCCTCAACC TTACCCATCC CACCGCCGCC 
CAGCTGAGTG CTAGTGGGCT GAGCGGGTCG AGTAAGACAA TCGACACGAT TAAGTTCCAG 
GAGAAGAGCA TGAAGACCGA GTCCAAGACC AAGGTGGTCA CCGATGGCTT CAGTTCCGAG 
CAGGCCACTA GCAATTCGGC GGAGATGAAA CGTTTGCAGG CCGGAGATAT AGACTATCAA 
GAAAGCAAGG GTGCGTCGGC GATGAGAAAT CGATTGGAGG TGGATGGGGT CAAAACGGAG 
GAGAATGCAG CCGTCATTAA GGAAGCCCTC TCACTCCGCA CCGGCGACAT CACACAGCAG 
GCGAGCAACA ATGTGGCGGC CTCCAGTATT ACGGTGCAAA GTGAGAATTT CTCCGCGGAC 
AAGAAGGCGA TATCCCAGTC GCAGCAATCG CAGACGATGA CCTCCAACGG GATCATCAGT 
CAGGAGAAAC ATGTATCCTC CGCCTCGCAG GCCAACTACT CGATGTCGCA CAAGGGCGTT 
TCCAGTACCG GCAGTAGCAT GATCACCAGC TCCTCGCAAA TGTCCGCGAT GAATGGCCAG 
ATGCTGAAGC TCGCCGATCT CAAGTTGGAT GACCTCAAGT CGCTGACAGC TGGCAGTGGG 
CAGCAGGAGA TCGAGCAGAC CATCAACAAG TATTCCAATA TGCTGACCTC AATAGTGAGC 
TCCCTGCAAG AAGATGAGCG AGGTGGTAGT GCCATAACCG TCCACGATGT TGGTGGCAAG 
AAGTCTCAGT ACCTGGAGAA GATCAACGAA GTCATTCGTC GAGCTTGGGC AGTGCCCACA 
CATGGCCACG AGCTGGGCTA CTCATTGTGC AATTCCCTGC GCCAAAGTGG CGGCCTCGAT 
CTTCTCATGA AGAACTGCGT TAAGCCCGAT CTCCAGTTTT CCTCCGCACA GCTTCTTGAG 
CAGTGCCTAA CCACCGAGAA CCGCAAACAT GTGGTGGATA ATGGTCTGGA TAAGGTGGTC 
AATGTGGCCT GTGTCTGCAC AAAGAATTCC AATATGGAGC ACTCCCGCGT GGGCACTGGC 
ATCCTGGAGC ATCTGTTCAA ACACTCCGAG GGCACCTGTT CGGATGTGAT AAGGCTAGGT 
GGTCTGGATG CCGTGCTCTT TGAGTGCCGC ACCAGTGATT TGGAAACTCT GCGACACTGC 
GCTAGTGCAC TGGCAAATTT ATCTCTATAT GGTGGAGCCG AGAATCAGGA GGAGATGATC 
CTACGCAAGG TGCCCATGTG GTTGTTCCCA TTGGCCTTCC ACAACGATGA TAACATCAAG 
TACTATGCCT GCTTGGCCAT TGCAGTGCTG GTAGCCAACA AGGAGATTGA AGCCGAGGTG 
CTGAAGTCTG GATGCTTGGA TTTGGTGGAA CCCTTTGTCA CCTCCCACGA CCCCTCGGCT 
TTTGCGAGAT CCAATCTGGC ACATGCCCAC GGGCAAAGCA AACATTGGCT GAAAAGATTG 
GTTCCAGTTT TGAGTTCCAA TCGCGAAGAG GCCCGCAATC TGGCTGCCTT CCATTTTTGC 
ATGGAGGCGG GCATCAAGAG GGAGCAGGGT AACACCGACA TCTTCCGCGA GATCAACGCT 
ATTGAAGCTT TGAAAAATGT GGCCAGCTGT CCAAATGCCA TTGCATCCAA GTTCGCCGCT 
CAGGCTCTGA GATTGATTGG AGAGACAGTT CCGCACAAGC TGTCCCAACA GGTTCCCTTG 
TGGTCCGTGG AGGATGTGCA GGAGTGGGTA AAGCAAATCG GTTTCAATGA CTACATCGAC 
AAGTTCAACG AGTCCCAGGT GGACGGGGAT CTTTTACTGA AGCTCAATCA GGATAACCTC 
CGGGCTGATA TTGGAATCGG TAATGGAATA CTTCTAAAGC GTTTTGAACG CGAGCTGCAA 
AATCTCAAGC GTATGGCTGA CTACTCATCC AAGGACACGG CCAAGATGCA CCAATTTCTC 
TCGGAGATCG GTACTGATTA CTGCACCTAC ACGTATGCCA TGCTGAATGC TGGAATTGAC 
AAGTGTGCAC TGCCGCATGT CAATGAGGAT ATGCTGATGA CAGAGTGTGG CATCCACAAC 
TCGATCCATC GTCTGAGAAT TCTCAATGCG GTCAAAAACT TGGAGAATTC GCTGCCCAGC 
TCGTCGGAGG AGAACATGGC CAAGACGCTA GATGTTTTCG TTAGCTATAG GCGATCAAAC 
GGCTCCCAAC TAGCCAGTCT TCTCAAGGTG CATCTGCAGC TTCGTGGTTT CTCCGTTTTC 
ATCGACGTGG AGCGCCTGGA GGCGGGCAAA TTTGACAACG GCCTATTGAA CAGTATTCGA 
CAGGCAAAGA ACTTTGTCTT AGTATTAACT CCCGATGCCC TGCACCGCTG CATTAACGAC 
GAGGACTGCA AGGACTGGGT GCATCGCGAA ATCGTGGCTG CCCTGAACTC GAACTGCAAC 
ATTATACCCA TCATAGACCA GCAATTCGAT TGGCCCGAGG TGGAGCGACT TCCCGAGGAC 
ATGCGCAGTG TGGCCCATTT TAACGGTGTT AATTGGATCC ACGACTACCA GGACGCATGC 
ATCGATAAGC TCGAAAGATT TTTGCGCGGC GAAAAGAATA TCGATCGCAT TGCGGCGATG 
GTGCCCGGCA CCCCCGGTTC GGTCTCATAC CAAAGAATGC ACAGCAACGA CTCCGACTAC 
CAGAGCGGAG GAGCAGGCGC CGGATCAGGA GCAGGAACTG GAGGAGGAGG TGGTGGCGGC 
GTTACGGGCA GTGTGGTGGA TGGCCTGATG GTGGCGGCCA ATGGCAGCGG ACAAGCTAAT 
CACCAGGCAA ATAGGTACCG CCAATCTCCC TCACCGGCCC GCCAAAGGGG CAGCACCTCG 
CAGTTGAGTG GTTATTCTCG GGCGCCCTCG AAACGCTCCC AAATCCTCAC CCCGTATCGC 
ACCCAACAGG CAGCCCTGCT CCACAAAACC GGAGCGGGTT CCGCGTCCAT GCAGAATATG 
ATGCCATTGG CGTATCTGCC TCCGCGGAGG AGTTCCGCCG CGGGATTAGG TCATGGATCT 
GGTTCCGGCA TGGGCAGTGG CTATCGATCG CACAGTGTGG ATGGGCTTCT AGATCAGGCG 
GGCTCAACTC CAGAGCAAAG AATAGCAGCA GCTGCGGCCA AGGTGACAGC GGGTAGTACA 
GCTCTAACGA ACGCCAGTTC CACAAGCACT CTGCAGCCCG AAGAAGAGGT GACGGATGCC 
GCCCTCAATG ACTCGGTGAC CCGGCGTGAC AAGCATACTT TATCCCCACC GGGAAATGTA 
CAGCAGCACA GAAAGTCCCG AAGTCTGGAT CATATTCTCA GCAAACAGAC TTTAGCAGAG 
CTGCTTCCAC CGAGTAGCGA ACTCGCAGAT GGAACGCAAA GTATGCAAAA CCTGGCCATT 
CCAATGACCC CGCAGCCCCA ACGAAGGGAC ACCAGCTCCT CCTCGAAATC CCCCACTCCC 
GAAAGACCTC CGCAACCAGC GATGGAAAGG GTAAGGGAAC GCCAGAGTCC TGAGGGTGTG 
AGTGCGACGG AAAGCGAGCG GGAGGATCAG CCAGAAGAAT GTCTGCGACA TGGCAACCAA 
CAGAGGGCAT CTGCCTCGGT TCATCGGGGA GCCAGTTTGA CCAGCAACAA GACCTCCAAC 
TCCTCACTGG GCTCCAACTT TAGTGCCGGA GGAAACAACA AGACGATATT TAATCGAACG 
ATGAAGAAGG TCCGCTCGCT GATAAAAAAC AACGAAATGG AGGACGAGGA ACTTTCGGGC 
ATCATTCTGT CCAAGGCCAC CTCCCCCAAT GCCGGGCGCA TGATATTTTG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ect4 ( NM_001104074.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097544.1 Drosophila melanogaster Ectoderm-expressed 4 (Ect4), transcript variant G, mRNA.

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CloneID	OFa17860
Clone ID Related Accession (Same CDS sequence)	NM_001104074.3
Accession Version	NM_001104074.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4689bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ectoderm-expressed 4, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001104074.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTTGTCA CATCGACCAG AAGTCCGCTG CTGGCGCAAA CCCAACCGGC AAATGCCTCA 
ACCAGTGGCA CTCACACCTC GGCCGCCCTG CGATCGCGTC TCATATTTCC CCGGCAGCAT 
TATCAGATGA CAGACTTCTC GGATAGCTCG CCAAGTGGGG TGCTGAGTCC ACCCACCTCG 
CTGATCGAGA ATTTCCACAA GAAGAACAGC TCACCGCAGC CACTTGGCAG TAGCAGCGCC 
ATAAGCACCC TAACTCCAAC AACCTTGAAT GGATCTGTGG TTGGCGGTGG AGTGGGTGGT 
GCTGGACACC ACAGCATCGA GAATGGCAGG CAAATCAATA CCAGCTCTAG TTCATGCAGT 
AGTCTTAAGG AAACCTCACA CCAGTCCACA TCCACATCAC AACAGGTTGT CAACAGCAGC 
AGCAGCACAA CGACGCGGGT GGAGAAGAAG TCTCAGCGTT TGCACCACCA CATAACCTCC 
TCATCCTCCA GCTCTTCAAC CACCCAGGCT CTGTCCACCT CATCAGAGAT GAAGGCGGCG 
GAAATCAAGC GAGATCTCAC AAACATCCAG AAGTCCATGT CGGAAATCAA CGATCTGGCC 
AAGGAGCGGA TCACCGGCGG ACCTGGATCC ATTAGCACCA CCTCAGCCTC GGCGATCACA 
GCACCCAGCA CGATGTCCCA GACGACGACG AGCCGGCTGG CACCCAAACT CACATCCGCC 
CATCCGTCAA TCGACGATCT CAGGGGTCTC AGTAGGCAGG ACAAAATCAC CCAGCTGCAA 
AAAAAAATTC GCGCCTCGTT CGAGAACCTG GTGGACCACG ATGACAGCAA TGTGATCGTG 
ACCCTGCCGG ATGACGACGA CTGCCCGCAT AACCATTTTG GCAGCGGCCT CAACCTTACC 
CATCCCACCG CCGCCCAGCT GAGTGCTAGT GGGCTGAGCG GGTCGAGTAA GACAATCGAC 
ACGATTAAGT TCCAGGAGAA GAGCATGAAG ACCGAGTCCA AGACCAAGGT GGTCACCGAT 
GGCTTCAGTT CCGAGCAGGC CACTAGCAAT TCGGCGGAGA TGAAACGTTT GCAGGCCGGA 
GATATAGACT ATCAAGAAAG CAAGGGTGCG TCGGCGATGA GAAATCGATT GGAGGTGGAT 
GGGGTCAAAA CGGAGGAGAA TGCAGCCGTC ATTAAGGAAG CCCTCTCACT CCGCACCGGC 
GACATCACAC AGCAGGCGAG CAACAATGTG GCGGCCTCCA GTATTACGGT GCAAAGTGAG 
AATTTCTCCG CGGACAAGAA GGCGATATCC CAGTCGCAGC AATCGCAGAC GATGACCTCC 
AACGGGATCA TCAGTCAGGA GAAACATGTA TCCTCCGCCT CGCAGGCCAA CTACTCGATG 
TCGCACAAGG GCGTTTCCAG TACCGGCAGT AGCATGATCA CCAGCTCCTC GCAAATGTCC 
GCGATGAATG GCCAGATGCT GAAGCTCGCC GATCTCAAGT TGGATGACCT CAAGTCGCTG 
ACAGCTGGCA GTGGGCAGCA GGAGATCGAG CAGACCATCA ACAAGTATTC CAATATGCTG 
ACCTCAATAG TGAGCTCCCT GCAAGAAGAT GAGCGAGGTG GTAGTGCCAT AACCGTCCAC 
GATGTTGGTG GCAAGAAGTC TCAGTACCTG GAGAAGATCA ACGAAGTCAT TCGTCGAGCT 
TGGGCAGTGC CCACACATGG CCACGAGCTG GGCTACTCAT TGTGCAATTC CCTGCGCCAA 
AGTGGCGGCC TCGATCTTCT CATGAAGAAC TGCGTTAAGC CCGATCTCCA GTTTTCCTCC 
GCACAGCTTC TTGAGCAGTG CCTAACCACC GAGAACCGCA AACATGTGGT GGATAATGGT 
CTGGATAAGG TGGTCAATGT GGCCTGTGTC TGCACAAAGA ATTCCAATAT GGAGCACTCC 
CGCGTGGGCA CTGGCATCCT GGAGCATCTG TTCAAACACT CCGAGGGCAC CTGTTCGGAT 
GTGATAAGGC TAGGTGGTCT GGATGCCGTG CTCTTTGAGT GCCGCACCAG TGATTTGGAA 
ACTCTGCGAC ACTGCGCTAG TGCACTGGCA AATTTATCTC TATATGGTGG AGCCGAGAAT 
CAGGAGGAGA TGATCCTACG CAAGGTGCCC ATGTGGTTGT TCCCATTGGC CTTCCACAAC 
GATGATAACA TCAAGTACTA TGCCTGCTTG GCCATTGCAG TGCTGGTAGC CAACAAGGAG 
ATTGAAGCCG AGGTGCTGAA GTCTGGATGC TTGGATTTGG TGGAACCCTT TGTCACCTCC 
CACGACCCCT CGGCTTTTGC GAGATCCAAT CTGGCACATG CCCACGGGCA AAGCAAACAT 
TGGCTGAAAA GATTGGTTCC AGTTTTGAGT TCCAATCGCG AAGAGGCCCG CAATCTGGCT 
GCCTTCCATT TTTGCATGGA GGCGGGCATC AAGAGGGAGC AGGGTAACAC CGACATCTTC 
CGCGAGATCA ACGCTATTGA AGCTTTGAAA AATGTGGCCA GCTGTCCAAA TGCCATTGCA 
TCCAAGTTCG CCGCTCAGGC TCTGAGATTG ATTGGAGAGA CAGTTCCGCA CAAGCTGTCC 
CAACAGGTTC CCTTGTGGTC CGTGGAGGAT GTGCAGGAGT GGGTAAAGCA AATCGGTTTC 
AATGACTACA TCGACAAGTT CAACGAGTCC CAGGTGGACG GGGATCTTTT ACTGAAGCTC 
AATCAGGATA ACCTCCGGGC TGATATTGGA ATCGGTAATG GAATACTTCT AAAGCGTTTT 
GAACGCGAGC TGCAAAATCT CAAGCGTATG GCTGACTACT CATCCAAGGA CACGGCCAAG 
ATGCACCAAT TTCTCTCGGA GATCGGTACT GATTACTGCA CCTACACGTA TGCCATGCTG 
AATGCTGGAA TTGACAAGTG TGCACTGCCG CATGTCAATG AGGATATGCT GATGACAGAG 
TGTGGCATCC ACAACTCGAT CCATCGTCTG AGAATTCTCA ATGCGGTCAA AAACTTGGAG 
AATTCGCTGC CCAGCTCGTC GGAGGAGAAC ATGGCCAAGA CGCTAGATGT TTTCGTTAGC 
TATAGGCGAT CAAACGGCTC CCAACTAGCC AGTCTTCTCA AGGTGCATCT GCAGCTTCGT 
GGTTTCTCCG TTTTCATCGA CGTGGAGCGC CTGGAGGCGG GCAAATTTGA CAACGGCCTA 
TTGAACAGTA TTCGACAGGC AAAGAACTTT GTCTTAGTAT TAACTCCCGA TGCCCTGCAC 
CGCTGCATTA ACGACGAGGA CTGCAAGGAC TGGGTGCATC GCGAAATCGT GGCTGCCCTG 
AACTCGAACT GCAACATTAT ACCCATCATA GACCAGCAAT TCGATTGGCC CGAGGTGGAG 
CGACTTCCCG AGGACATGCG CAGTGTGGCC CATTTTAACG GTGTTAATTG GATCCACGAC 
TACCAGGACG CATGCATCGA TAAGCTCGAA AGATTTTTGC GCGGCGAAAA GAATATCGAT 
CGCATTGCGG CGATGGTGCC CGGCACCCCC GGTTCGGTCT CATACCAAAG AATGCACAGC 
AACGACTCCG ACTACCAGAG CGGAGGAGCA GGCGCCGGAT CAGGAGCAGG AACTGGAGGA 
GGAGGTGGTG GCGGCGTTAC GGGCAGTGTG GTGGATGGCC TGATGGTGGC GGCCAATGGC 
AGCGGACAAG CTAATCACCA GGCAAATAGG TACCGCCAAT CTCCCTCACC GGCCCGCCAA 
AGGGGCAGCA CCTCGCAGTT GAGTGGTTAT TCTCGGGCGC CCTCGAAACG CTCCCAAATC 
CTCACCCCGT ATCGCACCCA ACAGGCAGCC CTGCTCCACA AAACCGGAGC GGGTTCCGCG 
TCCATGCAGA ATATGATGCC ATTGGCGTAT CTGCCTCCGC GGAGGAGTTC CGCCGCGGGA 
TTAGGTCATG GATCTGGTTC CGGCATGGGC AGTGGCTATC GATCGCACAG TGTGGATGGG 
CTTCTAGATC AGGCGGGCTC AACTCCAGAG CAAAGAATAG CAGCAGCTGC GGCCAAGGTG 
ACAGCGGGTA GTACAGCTCT AACGAACGCC AGTTCCACAA GCACTCTGCA GCCCGAAGAA 
GAGGTGACGG ATGCCGCCCT CAATGACTCG GTGACCCGGC GTGACAAGCA TACTTTATCC 
CCACCGGGAA ATGTACAGCA GCACAGAAAG TCCCGAAGTC TGGATCATAT TCTCAGCAAA 
CAGACTTTAG CAGAGCTGCT TCCACCGAGT AGCGAACTCG CAGATGGAAC GCAAAGTATG 
CAAAACCTGG CCATTCCAAT GACCCCGCAG CCCCAACGAA GGGACACCAG CTCCTCCTCG 
AAATCCCCCA CTCCCGAAAG ACCTCCGCAA CCAGCGATGG AAAGGGTAAG GGAACGCCAG 
AGTCCTGAGG GTGTGAGTGC GACGGAAAGC GAGCGGGAGG ATCAGCCAGA AGAATGTCTG 
CGACATGGCA ACCAACAGAG GGCATCTGCC TCGGTTCATC GGGGAGCCAG TTTGACCAGC 
AACAAGACCT CCAACTCCTC ACTGGGCTCC AACTTTAGTG CCGGAGGAAA CAACAAGACG 
ATATTTAATC GAACGATGAA GAAGGTCCGC TCGCTGATAA AAAACAACGA AATGGAGGAC 
GAGGAACTTT CGGGCATCAT TCTGTCCAAG GCCACCTCCC CCAATGCCGG GCGCATGATA 
TTTTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097544.1
CDS	329..5017
Translation	MVVTSTRSPLLAQTQPANASTSGTHTSAALRSRLIFPRQHYQMTDFSDSSPSGVLSPPTSLIENFHKKNSSPQPLGSSSAISTLTPTTLNGSVVGGGVGGAGHHSIENGRQINTSSSSCSSLKETSHQSTSTSQQVVNSSSSTTTRVEKKSQRLHHHITSSSSSSSTTQALSTSSEMKAAEIKRDLTNIQKSMSEINDLAKERITGGPGSISTTSASAITAPSTMSQTTTSRLAPKLTSAHPSIDDLRGLSRQDKITQLQKKIRASFENLVDHDDSNVIVTLPDDDDCPHNHFGSGLNLTHPTAAQLSASGLSGSSKTIDTIKFQEKSMKTESKTKVVTDGFSSEQATSNSAEMKRLQAGDIDYQESKGASAMRNRLEVDGVKTEENAAVIKEALSLRTGDITQQASNNVAASSITVQSENFSADKKAISQSQQSQTMTSNGIISQEKHVSSASQANYSMSHKGVSSTGSSMITSSSQMSAMNGQMLKLADLKLDDLKSLTAGSGQQEIEQTINKYSNMLTSIVSSLQEDERGGSAITVHDVGGKKSQYLEKINEVIRRAWAVPTHGHELGYSLCNSLRQSGGLDLLMKNCVKPDLQFSSAQLLEQCLTTENRKHVVDNGLDKVVNVACVCTKNSNMEHSRVGTGILEHLFKHSEGTCSDVIRLGGLDAVLFECRTSDLETLRHCASALANLSLYGGAENQEEMILRKVPMWLFPLAFHNDDNIKYYACLAIAVLVANKEIEAEVLKSGCLDLVEPFVTSHDPSAFARSNLAHAHGQSKHWLKRLVPVLSSNREEARNLAAFHFCMEAGIKREQGNTDIFREINAIEALKNVASCPNAIASKFAAQALRLIGETVPHKLSQQVPLWSVEDVQEWVKQIGFNDYIDKFNESQVDGDLLLKLNQDNLRADIGIGNGILLKRFERELQNLKRMADYSSKDTAKMHQFLSEIGTDYCTYTYAMLNAGIDKCALPHVNEDMLMTECGIHNSIHRLRILNAVKNLENSLPSSSEENMAKTLDVFVSYRRSNGSQLASLLKVHLQLRGFSVFIDVERLEAGKFDNGLLNSIRQAKNFVLVLTPDALHRCINDEDCKDWVHREIVAALNSNCNIIPIIDQQFDWPEVERLPEDMRSVAHFNGVNWIHDYQDACIDKLERFLRGEKNIDRIAAMVPGTPGSVSYQRMHSNDSDYQSGGAGAGSGAGTGGGGGGGVTGSVVDGLMVAANGSGQANHQANRYRQSPSPARQRGSTSQLSGYSRAPSKRSQILTPYRTQQAALLHKTGAGSASMQNMMPLAYLPPRRSSAAGLGHGSGSGMGSGYRSHSVDGLLDQAGSTPEQRIAAAAAKVTAGSTALTNASSTSTLQPEEEVTDAALNDSVTRRDKHTLSPPGNVQQHRKSRSLDHILSKQTLAELLPPSSELADGTQSMQNLAIPMTPQPQRRDTSSSSKSPTPERPPQPAMERVRERQSPEGVSATESEREDQPEECLRHGNQQRASASVHRGASLTSNKTSNSSLGSNFSAGGNNKTIFNRTMKKVRSLIKNNEMEDEELSGIILSKATSPNAGRMIFW

Target ORF information:

RefSeq Version	NM_001104074.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ectoderm-expressed 4 (Ect4), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001104074.3

ORF Insert Sequence:

ATGGTTGTCA CATCGACCAG AAGTCCGCTG CTGGCGCAAA CCCAACCGGC AAATGCCTCA 
ACCAGTGGCA CTCACACCTC GGCCGCCCTG CGATCGCGTC TCATATTTCC CCGGCAGCAT 
TATCAGATGA CAGACTTCTC GGATAGCTCG CCAAGTGGGG TGCTGAGTCC ACCCACCTCG 
CTGATCGAGA ATTTCCACAA GAAGAACAGC TCACCGCAGC CACTTGGCAG TAGCAGCGCC 
ATAAGCACCC TAACTCCAAC AACCTTGAAT GGATCTGTGG TTGGCGGTGG AGTGGGTGGT 
GCTGGACACC ACAGCATCGA GAATGGCAGG CAAATCAATA CCAGCTCTAG TTCATGCAGT 
AGTCTTAAGG AAACCTCACA CCAGTCCACA TCCACATCAC AACAGGTTGT CAACAGCAGC 
AGCAGCACAA CGACGCGGGT GGAGAAGAAG TCTCAGCGTT TGCACCACCA CATAACCTCC 
TCATCCTCCA GCTCTTCAAC CACCCAGGCT CTGTCCACCT CATCAGAGAT GAAGGCGGCG 
GAAATCAAGC GAGATCTCAC AAACATCCAG AAGTCCATGT CGGAAATCAA CGATCTGGCC 
AAGGAGCGGA TCACCGGCGG ACCTGGATCC ATTAGCACCA CCTCAGCCTC GGCGATCACA 
GCACCCAGCA CGATGTCCCA GACGACGACG AGCCGGCTGG CACCCAAACT CACATCCGCC 
CATCCGTCAA TCGACGATCT CAGGGGTCTC AGTAGGCAGG ACAAAATCAC CCAGCTGCAA 
AAAAAAATTC GCGCCTCGTT CGAGAACCTG GTGGACCACG ATGACAGCAA TGTGATCGTG 
ACCCTGCCGG ATGACGACGA CTGCCCGCAT AACCATTTTG GCAGCGGCCT CAACCTTACC 
CATCCCACCG CCGCCCAGCT GAGTGCTAGT GGGCTGAGCG GGTCGAGTAA GACAATCGAC 
ACGATTAAGT TCCAGGAGAA GAGCATGAAG ACCGAGTCCA AGACCAAGGT GGTCACCGAT 
GGCTTCAGTT CCGAGCAGGC CACTAGCAAT TCGGCGGAGA TGAAACGTTT GCAGGCCGGA 
GATATAGACT ATCAAGAAAG CAAGGGTGCG TCGGCGATGA GAAATCGATT GGAGGTGGAT 
GGGGTCAAAA CGGAGGAGAA TGCAGCCGTC ATTAAGGAAG CCCTCTCACT CCGCACCGGC 
GACATCACAC AGCAGGCGAG CAACAATGTG GCGGCCTCCA GTATTACGGT GCAAAGTGAG 
AATTTCTCCG CGGACAAGAA GGCGATATCC CAGTCGCAGC AATCGCAGAC GATGACCTCC 
AACGGGATCA TCAGTCAGGA GAAACATGTA TCCTCCGCCT CGCAGGCCAA CTACTCGATG 
TCGCACAAGG GCGTTTCCAG TACCGGCAGT AGCATGATCA CCAGCTCCTC GCAAATGTCC 
GCGATGAATG GCCAGATGCT GAAGCTCGCC GATCTCAAGT TGGATGACCT CAAGTCGCTG 
ACAGCTGGCA GTGGGCAGCA GGAGATCGAG CAGACCATCA ACAAGTATTC CAATATGCTG 
ACCTCAATAG TGAGCTCCCT GCAAGAAGAT GAGCGAGGTG GTAGTGCCAT AACCGTCCAC 
GATGTTGGTG GCAAGAAGTC TCAGTACCTG GAGAAGATCA ACGAAGTCAT TCGTCGAGCT 
TGGGCAGTGC CCACACATGG CCACGAGCTG GGCTACTCAT TGTGCAATTC CCTGCGCCAA 
AGTGGCGGCC TCGATCTTCT CATGAAGAAC TGCGTTAAGC CCGATCTCCA GTTTTCCTCC 
GCACAGCTTC TTGAGCAGTG CCTAACCACC GAGAACCGCA AACATGTGGT GGATAATGGT 
CTGGATAAGG TGGTCAATGT GGCCTGTGTC TGCACAAAGA ATTCCAATAT GGAGCACTCC 
CGCGTGGGCA CTGGCATCCT GGAGCATCTG TTCAAACACT CCGAGGGCAC CTGTTCGGAT 
GTGATAAGGC TAGGTGGTCT GGATGCCGTG CTCTTTGAGT GCCGCACCAG TGATTTGGAA 
ACTCTGCGAC ACTGCGCTAG TGCACTGGCA AATTTATCTC TATATGGTGG AGCCGAGAAT 
CAGGAGGAGA TGATCCTACG CAAGGTGCCC ATGTGGTTGT TCCCATTGGC CTTCCACAAC 
GATGATAACA TCAAGTACTA TGCCTGCTTG GCCATTGCAG TGCTGGTAGC CAACAAGGAG 
ATTGAAGCCG AGGTGCTGAA GTCTGGATGC TTGGATTTGG TGGAACCCTT TGTCACCTCC 
CACGACCCCT CGGCTTTTGC GAGATCCAAT CTGGCACATG CCCACGGGCA AAGCAAACAT 
TGGCTGAAAA GATTGGTTCC AGTTTTGAGT TCCAATCGCG AAGAGGCCCG CAATCTGGCT 
GCCTTCCATT TTTGCATGGA GGCGGGCATC AAGAGGGAGC AGGGTAACAC CGACATCTTC 
CGCGAGATCA ACGCTATTGA AGCTTTGAAA AATGTGGCCA GCTGTCCAAA TGCCATTGCA 
TCCAAGTTCG CCGCTCAGGC TCTGAGATTG ATTGGAGAGA CAGTTCCGCA CAAGCTGTCC 
CAACAGGTTC CCTTGTGGTC CGTGGAGGAT GTGCAGGAGT GGGTAAAGCA AATCGGTTTC 
AATGACTACA TCGACAAGTT CAACGAGTCC CAGGTGGACG GGGATCTTTT ACTGAAGCTC 
AATCAGGATA ACCTCCGGGC TGATATTGGA ATCGGTAATG GAATACTTCT AAAGCGTTTT 
GAACGCGAGC TGCAAAATCT CAAGCGTATG GCTGACTACT CATCCAAGGA CACGGCCAAG 
ATGCACCAAT TTCTCTCGGA GATCGGTACT GATTACTGCA CCTACACGTA TGCCATGCTG 
AATGCTGGAA TTGACAAGTG TGCACTGCCG CATGTCAATG AGGATATGCT GATGACAGAG 
TGTGGCATCC ACAACTCGAT CCATCGTCTG AGAATTCTCA ATGCGGTCAA AAACTTGGAG 
AATTCGCTGC CCAGCTCGTC GGAGGAGAAC ATGGCCAAGA CGCTAGATGT TTTCGTTAGC 
TATAGGCGAT CAAACGGCTC CCAACTAGCC AGTCTTCTCA AGGTGCATCT GCAGCTTCGT 
GGTTTCTCCG TTTTCATCGA CGTGGAGCGC CTGGAGGCGG GCAAATTTGA CAACGGCCTA 
TTGAACAGTA TTCGACAGGC AAAGAACTTT GTCTTAGTAT TAACTCCCGA TGCCCTGCAC 
CGCTGCATTA ACGACGAGGA CTGCAAGGAC TGGGTGCATC GCGAAATCGT GGCTGCCCTG 
AACTCGAACT GCAACATTAT ACCCATCATA GACCAGCAAT TCGATTGGCC CGAGGTGGAG 
CGACTTCCCG AGGACATGCG CAGTGTGGCC CATTTTAACG GTGTTAATTG GATCCACGAC 
TACCAGGACG CATGCATCGA TAAGCTCGAA AGATTTTTGC GCGGCGAAAA GAATATCGAT 
CGCATTGCGG CGATGGTGCC CGGCACCCCC GGTTCGGTCT CATACCAAAG AATGCACAGC 
AACGACTCCG ACTACCAGAG CGGAGGAGCA GGCGCCGGAT CAGGAGCAGG AACTGGAGGA 
GGAGGTGGTG GCGGCGTTAC GGGCAGTGTG GTGGATGGCC TGATGGTGGC GGCCAATGGC 
AGCGGACAAG CTAATCACCA GGCAAATAGG TACCGCCAAT CTCCCTCACC GGCCCGCCAA 
AGGGGCAGCA CCTCGCAGTT GAGTGGTTAT TCTCGGGCGC CCTCGAAACG CTCCCAAATC 
CTCACCCCGT ATCGCACCCA ACAGGCAGCC CTGCTCCACA AAACCGGAGC GGGTTCCGCG 
TCCATGCAGA ATATGATGCC ATTGGCGTAT CTGCCTCCGC GGAGGAGTTC CGCCGCGGGA 
TTAGGTCATG GATCTGGTTC CGGCATGGGC AGTGGCTATC GATCGCACAG TGTGGATGGG 
CTTCTAGATC AGGCGGGCTC AACTCCAGAG CAAAGAATAG CAGCAGCTGC GGCCAAGGTG 
ACAGCGGGTA GTACAGCTCT AACGAACGCC AGTTCCACAA GCACTCTGCA GCCCGAAGAA 
GAGGTGACGG ATGCCGCCCT CAATGACTCG GTGACCCGGC GTGACAAGCA TACTTTATCC 
CCACCGGGAA ATGTACAGCA GCACAGAAAG TCCCGAAGTC TGGATCATAT TCTCAGCAAA 
CAGACTTTAG CAGAGCTGCT TCCACCGAGT AGCGAACTCG CAGATGGAAC GCAAAGTATG 
CAAAACCTGG CCATTCCAAT GACCCCGCAG CCCCAACGAA GGGACACCAG CTCCTCCTCG 
AAATCCCCCA CTCCCGAAAG ACCTCCGCAA CCAGCGATGG AAAGGGTAAG GGAACGCCAG 
AGTCCTGAGG GTGTGAGTGC GACGGAAAGC GAGCGGGAGG ATCAGCCAGA AGAATGTCTG 
CGACATGGCA ACCAACAGAG GGCATCTGCC TCGGTTCATC GGGGAGCCAG TTTGACCAGC 
AACAAGACCT CCAACTCCTC ACTGGGCTCC AACTTTAGTG CCGGAGGAAA CAACAAGACG 
ATATTTAATC GAACGATGAA GAAGGTCCGC TCGCTGATAA AAAACAACGA AATGGAGGAC 
GAGGAACTTT CGGGCATCAT TCTGTCCAAG GCCACCTCCC CCAATGCCGG GCGCATGATA 
TTTTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ect4 ( NM_001104075.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097545.1 Drosophila melanogaster Ectoderm-expressed 4 (Ect4), transcript variant H, mRNA.

Price & Availability↑

CloneID	OFa17861
Clone ID Related Accession (Same CDS sequence)	NM_001104075.2
Accession Version	NM_001104075.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ectoderm-expressed 4, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On May 8, 2012 this sequence version replaced NM_001104075.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGGCGG CGGAAATCAA GCGAGATCTC ACAAACATCC AGAAGTCCAT GTCGGAAATC 
AACGATCTGG CCAAGGAGCG GATCACCGGC GGACCTGGAT CCATTAGCAC CACCTCAGCC 
TCGGCGATCA CAGCACCCAG CACGATGTCC CAGACGACGA CGAGCCGGCT GGCACCCAAA 
CTCACATCCG CCCATCCGTC AATCGACGAT CTCAGGGGTC TCAGTAGGCA GGACAAAATC 
ACCCAGCTGC AAAAAAAAAT TCGCGCCTCG TTCGAGAACC TGGTGGACCA CGATGACAGC 
AATGTGATCG TGACCCTGCC GGATGACGAC GACTGCCCGC ATAACCATTT TGGCAGCGGC 
CTCAACCTTA CCCATCCCAC CGCCGCCCAG CTGAGTGCTA GTGGGCTGAG CGGGTCGAGT 
AAGACAATCG ACACGATTAA GTTCCAGGAG AAGAGCATGA AGACCGAGTC CAAGACCAAG 
GTGGTCACCG ATGGCTTCAG TTCCGAGCAG GCCACTAGCA ATTCGGCGGA GATGAAACGT 
TTGCAGGCCG GAGATATAGA CTATCAAGAA AGCAAGGGTG CGTCGGCGAT GAGAAATCGA 
TTGGAGGTGG ATGGGGTCAA AACGGAGGAG AATGCAGCCG TCATTAAGGA AGCCCTCTCA 
CTCCGCACCG GCGACATCAC ACAGCAGGCG AGCAACAATG TGGCGGCCTC CAGTATTACG 
GTGCAAAGTG AGAATTTCTC CGCGGACAAG AAGGCGATAT CCCAGTCGCA GCAATCGCAG 
ACGATGACCT CCAACGGGAT CATCAGTCAG GAGAAACATG TATCCTCCGC CTCGCAGGCC 
AACTACTCGA TGTCGCACAA GGGCGTTTCC AGTACCGGCA GTAGCATGAT CACCAGCTCC 
TCGCAAATGT CCGCGATGAA TGGCCAGATG CTGAAGCTCG CCGATCTCAA GTTGGATGAC 
CTCAAGTCGC TGACAGCTGG CAGTGGGCAG CAGGAGATCG AGCAGACCAT CAACAAGTAT 
TCCAATATGC TGACCTCAAT AGTGAGCTCC CTGCAAGAAG ATGAGCGAGG TGGTAGTGCC 
ATAACCGTCC ACGATGTTGG TGGCAAGAAG TCTCAGTACC TGGAGAAGAT CAACGAAGTC 
ATTCGTCGAG CTTGGGCAGT GCCCACACAT GGCCACGAGC TGGGCTACTC ATTGTGCAAT 
TCCCTGCGCC AAAGTGGCGG CCTCGATCTT CTCATGAAGA ACTGCGTTAA GCCCGATCTC 
CAGTTTTCCT CCGCACAGCT TCTTGAGCAG TGCCTAACCA CCGAGAACCG CAAACATGTG 
GTGGATAATG GTCTGGATAA GGTGGTCAAT GTGGCCTGTG TCTGCACAAA GAATTCCAAT 
ATGGAGCACT CCCGCGTGGG CACTGGCATC CTGGAGCATC TGTTCAAACA CTCCGAGGGC 
ACCTGTTCGG ATGTGATAAG GCTAGGTGGT CTGGATGCCG TGCTCTTTGA GTGCCGCACC 
AGTGATTTGG AAACTCTGCG ACACTGCGCT AGTGCACTGG CAAATTTATC TCTATATGGT 
GGAGCCGAGA ATCAGGAGGA GATGATCCTA CGCAAGGTGC CCATGTGGTT GTTCCCATTG 
GCCTTCCACA ACGATGATAA CATCAAGTAC TATGCCTGCT TGGCCATTGC AGTGCTGGTA 
GCCAACAAGG AGATTGAAGC CGAGGTGCTG AAGTCTGGAT GCTTGGATTT GGTGGAACCC 
TTTGTCACCT CCCACGACCC CTCGGCTTTT GCGAGATCCA ATCTGGCACA TGCCCACGGG 
CAAAGCAAAC ATTGGCTGAA AAGATTGGTT CCAGTTTTGA GTTCCAATCG CGAAGAGGCC 
CGCAATCTGG CTGCCTTCCA TTTTTGCATG GAGGCGGGCA TCAAGAGGGA GCAGGGTAAC 
ACCGACATCT TCCGCGAGAT CAACGCTATT GAAGCTTTGA AAAATGTGGC CAGCTGTCCA 
AATGCCATTG CATCCAAGTT CGCCGCTCAG GCTCTGAGAT TGATTGGAGA GACAGTTCCG 
CACAAGCTGT CCCAACAGGT TCCCTTGTGG TCCGTGGAGG ATGTGCAGGA GTGGGTAAAG 
CAAATCGGTT TCAATGACTA CATCGACAAG TTCAACGAGT CCCAGGTGGA CGGGGATCTT 
TTACTGAAGC TCAATCAGGA TAACCTCCGG GCTGATATTG GAATCGGTAA TGGAATACTT 
CTAAAGCGTT TTGAACGCGA GCTGCAAAAT CTCAAGCGTA TGGCTGACTA CTCATCCAAG 
GACACGGCCA AGATGCACCA ATTTCTCTCG GAGATCGGTA CTGATTACTG CACCTACACG 
TATGCCATGC TGAATGCTGG AATTGACAAG TGTGCACTGC CGCATGTCAA TGAGGATATG 
CTGATGACAG AGTGTGGCAT CCACAACTCG ATCCATCGTC TGAGAATTCT CAATGCGGTC 
AAAAACTTGG AGAATTCGCT GCCCAGCTCG TCGGAGGAGA ACATGGCCAA GACGCTAGAT 
GTTTTCGTTA GCTATAGGCG ATCAAACGGC TCCCAACTAG CCAGTCTTCT CAAGGTGCAT 
CTGCAGCTTC GTGGTTTCTC CGTTTTCATC GACGTGGAGC GCCTGGAGGC GGGCAAATTT 
GACAACGGCC TATTGAACAG TATTCGACAG GCAAAGAACT TTGTCTTAGT ATTAACTCCC 
GATGCCCTGC ACCGCTGCAT TAACGACGAG GACTGCAAGG ACTGGGTGCA TCGCGAAATC 
GTGGCTGCCC TGAACTCGAA CTGCAACATT ATACCCATCA TAGACCAGCA ATTCGATTGG 
CCCGAGGTGG AGCGACTTCC CGAGGACATG CGCAGTGTGG CCCATTTTAA CGGTGTTAAT 
TGGATCCACG ACTACCAGGA CGCATGCATC GATAAGCTCG AAAGATTTTT GCGCGGCGAA 
AAGAATATCG ATCGCATTGC GGCGATGGTG CCCGGCACCC CCGGTTCGGT CTCATACCAA 
AGAATGCACA GCAACGACTC CGACTACCAG AGCGGAGGAG CAGGCGCCGG ATCAGGAGCA 
GGAACTGGAG GAGGAGGTGG TGGCGGCGTT ACGGGCAGTG TGGTGGATGG CCTGATGGTG 
GCGGCCAATG GCAGCGGACA AGCTAATCAC CAGGCAAATA GGTACCGCCA ATCTCCCTCA 
CCGGCCCGCC AAAGGGGCAG CACCTCGCAG TTGAGTGGTT ATTCTCGGGC GCCCTCGAAA 
CGCTCCCAAA TCCTCACCCC GTATCGCACC CAACAGGCAG CCCTGCTCCA CAAAACCGGA 
GCGGGTTCCG CGTCCATGCA GAATATGATG CCATTGGCGT ATCTGCCTCC GCGGAGGAGT 
TCCGCCGCGG GATTAGGTCA TGGATCTGGT TCCGGCATGG GCAGTGGCTA TCGATCGCAC 
AGTGTGGATG GGCTTCTAGA TCAGGCGGGC TCAACTCCAG AGCAAAGAAT AGCAGCAGCT 
GCGGCCAAGG TGACAGCGGG TAGTACAGCT CTAACGAACG CCAGTTCCAC AAGCACTCTG 
CAGCCCGAAG AAGAGGTGAC GGATGCCGCC CTCAATGACT CGGTGACCCG GCGTGACAAG 
CATACTTTAT CCCCACCGGG AAATGTACAG CAGCACAGAA AGTCCCGAAG TCTGGATCAT 
ATTCTCAGCA AACAGACTTT AGCAGAGCTG CTTCCACCGA GTAGCGAACT CGCAGATGGA 
ACGCAAAGTA TGCAAAACCT GGCCATTCCA ATGACCCCGC AGCCCCAACG AAGGGACACC 
AGCTCCTCCT CGAAATCCCC CACTCCCGAA AGACCTCCGC AACCAGCGAT GGAAAGGGTA 
AGGGAACGCC AGAGTCCTGA GGGTGTGAGT GCGACGGAAA GCGAGCGGGA GGATCAGCCA 
GAAGAATGTC TGCGACATGG CAACCAACAG AGGGCATCTG CCTCGGTTCA TCGGGGAGCC 
AGTTTGACCA GCAACAAGAC CTCCAACTCC TCACTGGGCT CCAACTTTAG TGCCGGAGGA 
AACAACAAGA CGATATTTAA TCGAACGATG AAGAAGGTCC GCTCGCTGAT AAAAAACAAC 
GAAATGGAGG ACGAGGAACT TTCGGGCATC ATTCTGTCCA AGGCCACCTC CCCCAATGCC 
GGGCGCATGA TATTTTGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097545.1
CDS	681..4841
Translation	MKAAEIKRDLTNIQKSMSEINDLAKERITGGPGSISTTSASAITAPSTMSQTTTSRLAPKLTSAHPSIDDLRGLSRQDKITQLQKKIRASFENLVDHDDSNVIVTLPDDDDCPHNHFGSGLNLTHPTAAQLSASGLSGSSKTIDTIKFQEKSMKTESKTKVVTDGFSSEQATSNSAEMKRLQAGDIDYQESKGASAMRNRLEVDGVKTEENAAVIKEALSLRTGDITQQASNNVAASSITVQSENFSADKKAISQSQQSQTMTSNGIISQEKHVSSASQANYSMSHKGVSSTGSSMITSSSQMSAMNGQMLKLADLKLDDLKSLTAGSGQQEIEQTINKYSNMLTSIVSSLQEDERGGSAITVHDVGGKKSQYLEKINEVIRRAWAVPTHGHELGYSLCNSLRQSGGLDLLMKNCVKPDLQFSSAQLLEQCLTTENRKHVVDNGLDKVVNVACVCTKNSNMEHSRVGTGILEHLFKHSEGTCSDVIRLGGLDAVLFECRTSDLETLRHCASALANLSLYGGAENQEEMILRKVPMWLFPLAFHNDDNIKYYACLAIAVLVANKEIEAEVLKSGCLDLVEPFVTSHDPSAFARSNLAHAHGQSKHWLKRLVPVLSSNREEARNLAAFHFCMEAGIKREQGNTDIFREINAIEALKNVASCPNAIASKFAAQALRLIGETVPHKLSQQVPLWSVEDVQEWVKQIGFNDYIDKFNESQVDGDLLLKLNQDNLRADIGIGNGILLKRFERELQNLKRMADYSSKDTAKMHQFLSEIGTDYCTYTYAMLNAGIDKCALPHVNEDMLMTECGIHNSIHRLRILNAVKNLENSLPSSSEENMAKTLDVFVSYRRSNGSQLASLLKVHLQLRGFSVFIDVERLEAGKFDNGLLNSIRQAKNFVLVLTPDALHRCINDEDCKDWVHREIVAALNSNCNIIPIIDQQFDWPEVERLPEDMRSVAHFNGVNWIHDYQDACIDKLERFLRGEKNIDRIAAMVPGTPGSVSYQRMHSNDSDYQSGGAGAGSGAGTGGGGGGGVTGSVVDGLMVAANGSGQANHQANRYRQSPSPARQRGSTSQLSGYSRAPSKRSQILTPYRTQQAALLHKTGAGSASMQNMMPLAYLPPRRSSAAGLGHGSGSGMGSGYRSHSVDGLLDQAGSTPEQRIAAAAAKVTAGSTALTNASSTSTLQPEEEVTDAALNDSVTRRDKHTLSPPGNVQQHRKSRSLDHILSKQTLAELLPPSSELADGTQSMQNLAIPMTPQPQRRDTSSSSKSPTPERPPQPAMERVRERQSPEGVSATESEREDQPEECLRHGNQQRASASVHRGASLTSNKTSNSSLGSNFSAGGNNKTIFNRTMKKVRSLIKNNEMEDEELSGIILSKATSPNAGRMIFW

Target ORF information:

RefSeq Version	NM_001104075.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ectoderm-expressed 4 (Ect4), transcript variant H, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001104075.2

ORF Insert Sequence:

ATGAAGGCGG CGGAAATCAA GCGAGATCTC ACAAACATCC AGAAGTCCAT GTCGGAAATC 
AACGATCTGG CCAAGGAGCG GATCACCGGC GGACCTGGAT CCATTAGCAC CACCTCAGCC 
TCGGCGATCA CAGCACCCAG CACGATGTCC CAGACGACGA CGAGCCGGCT GGCACCCAAA 
CTCACATCCG CCCATCCGTC AATCGACGAT CTCAGGGGTC TCAGTAGGCA GGACAAAATC 
ACCCAGCTGC AAAAAAAAAT TCGCGCCTCG TTCGAGAACC TGGTGGACCA CGATGACAGC 
AATGTGATCG TGACCCTGCC GGATGACGAC GACTGCCCGC ATAACCATTT TGGCAGCGGC 
CTCAACCTTA CCCATCCCAC CGCCGCCCAG CTGAGTGCTA GTGGGCTGAG CGGGTCGAGT 
AAGACAATCG ACACGATTAA GTTCCAGGAG AAGAGCATGA AGACCGAGTC CAAGACCAAG 
GTGGTCACCG ATGGCTTCAG TTCCGAGCAG GCCACTAGCA ATTCGGCGGA GATGAAACGT 
TTGCAGGCCG GAGATATAGA CTATCAAGAA AGCAAGGGTG CGTCGGCGAT GAGAAATCGA 
TTGGAGGTGG ATGGGGTCAA AACGGAGGAG AATGCAGCCG TCATTAAGGA AGCCCTCTCA 
CTCCGCACCG GCGACATCAC ACAGCAGGCG AGCAACAATG TGGCGGCCTC CAGTATTACG 
GTGCAAAGTG AGAATTTCTC CGCGGACAAG AAGGCGATAT CCCAGTCGCA GCAATCGCAG 
ACGATGACCT CCAACGGGAT CATCAGTCAG GAGAAACATG TATCCTCCGC CTCGCAGGCC 
AACTACTCGA TGTCGCACAA GGGCGTTTCC AGTACCGGCA GTAGCATGAT CACCAGCTCC 
TCGCAAATGT CCGCGATGAA TGGCCAGATG CTGAAGCTCG CCGATCTCAA GTTGGATGAC 
CTCAAGTCGC TGACAGCTGG CAGTGGGCAG CAGGAGATCG AGCAGACCAT CAACAAGTAT 
TCCAATATGC TGACCTCAAT AGTGAGCTCC CTGCAAGAAG ATGAGCGAGG TGGTAGTGCC 
ATAACCGTCC ACGATGTTGG TGGCAAGAAG TCTCAGTACC TGGAGAAGAT CAACGAAGTC 
ATTCGTCGAG CTTGGGCAGT GCCCACACAT GGCCACGAGC TGGGCTACTC ATTGTGCAAT 
TCCCTGCGCC AAAGTGGCGG CCTCGATCTT CTCATGAAGA ACTGCGTTAA GCCCGATCTC 
CAGTTTTCCT CCGCACAGCT TCTTGAGCAG TGCCTAACCA CCGAGAACCG CAAACATGTG 
GTGGATAATG GTCTGGATAA GGTGGTCAAT GTGGCCTGTG TCTGCACAAA GAATTCCAAT 
ATGGAGCACT CCCGCGTGGG CACTGGCATC CTGGAGCATC TGTTCAAACA CTCCGAGGGC 
ACCTGTTCGG ATGTGATAAG GCTAGGTGGT CTGGATGCCG TGCTCTTTGA GTGCCGCACC 
AGTGATTTGG AAACTCTGCG ACACTGCGCT AGTGCACTGG CAAATTTATC TCTATATGGT 
GGAGCCGAGA ATCAGGAGGA GATGATCCTA CGCAAGGTGC CCATGTGGTT GTTCCCATTG 
GCCTTCCACA ACGATGATAA CATCAAGTAC TATGCCTGCT TGGCCATTGC AGTGCTGGTA 
GCCAACAAGG AGATTGAAGC CGAGGTGCTG AAGTCTGGAT GCTTGGATTT GGTGGAACCC 
TTTGTCACCT CCCACGACCC CTCGGCTTTT GCGAGATCCA ATCTGGCACA TGCCCACGGG 
CAAAGCAAAC ATTGGCTGAA AAGATTGGTT CCAGTTTTGA GTTCCAATCG CGAAGAGGCC 
CGCAATCTGG CTGCCTTCCA TTTTTGCATG GAGGCGGGCA TCAAGAGGGA GCAGGGTAAC 
ACCGACATCT TCCGCGAGAT CAACGCTATT GAAGCTTTGA AAAATGTGGC CAGCTGTCCA 
AATGCCATTG CATCCAAGTT CGCCGCTCAG GCTCTGAGAT TGATTGGAGA GACAGTTCCG 
CACAAGCTGT CCCAACAGGT TCCCTTGTGG TCCGTGGAGG ATGTGCAGGA GTGGGTAAAG 
CAAATCGGTT TCAATGACTA CATCGACAAG TTCAACGAGT CCCAGGTGGA CGGGGATCTT 
TTACTGAAGC TCAATCAGGA TAACCTCCGG GCTGATATTG GAATCGGTAA TGGAATACTT 
CTAAAGCGTT TTGAACGCGA GCTGCAAAAT CTCAAGCGTA TGGCTGACTA CTCATCCAAG 
GACACGGCCA AGATGCACCA ATTTCTCTCG GAGATCGGTA CTGATTACTG CACCTACACG 
TATGCCATGC TGAATGCTGG AATTGACAAG TGTGCACTGC CGCATGTCAA TGAGGATATG 
CTGATGACAG AGTGTGGCAT CCACAACTCG ATCCATCGTC TGAGAATTCT CAATGCGGTC 
AAAAACTTGG AGAATTCGCT GCCCAGCTCG TCGGAGGAGA ACATGGCCAA GACGCTAGAT 
GTTTTCGTTA GCTATAGGCG ATCAAACGGC TCCCAACTAG CCAGTCTTCT CAAGGTGCAT 
CTGCAGCTTC GTGGTTTCTC CGTTTTCATC GACGTGGAGC GCCTGGAGGC GGGCAAATTT 
GACAACGGCC TATTGAACAG TATTCGACAG GCAAAGAACT TTGTCTTAGT ATTAACTCCC 
GATGCCCTGC ACCGCTGCAT TAACGACGAG GACTGCAAGG ACTGGGTGCA TCGCGAAATC 
GTGGCTGCCC TGAACTCGAA CTGCAACATT ATACCCATCA TAGACCAGCA ATTCGATTGG 
CCCGAGGTGG AGCGACTTCC CGAGGACATG CGCAGTGTGG CCCATTTTAA CGGTGTTAAT 
TGGATCCACG ACTACCAGGA CGCATGCATC GATAAGCTCG AAAGATTTTT GCGCGGCGAA 
AAGAATATCG ATCGCATTGC GGCGATGGTG CCCGGCACCC CCGGTTCGGT CTCATACCAA 
AGAATGCACA GCAACGACTC CGACTACCAG AGCGGAGGAG CAGGCGCCGG ATCAGGAGCA 
GGAACTGGAG GAGGAGGTGG TGGCGGCGTT ACGGGCAGTG TGGTGGATGG CCTGATGGTG 
GCGGCCAATG GCAGCGGACA AGCTAATCAC CAGGCAAATA GGTACCGCCA ATCTCCCTCA 
CCGGCCCGCC AAAGGGGCAG CACCTCGCAG TTGAGTGGTT ATTCTCGGGC GCCCTCGAAA 
CGCTCCCAAA TCCTCACCCC GTATCGCACC CAACAGGCAG CCCTGCTCCA CAAAACCGGA 
GCGGGTTCCG CGTCCATGCA GAATATGATG CCATTGGCGT ATCTGCCTCC GCGGAGGAGT 
TCCGCCGCGG GATTAGGTCA TGGATCTGGT TCCGGCATGG GCAGTGGCTA TCGATCGCAC 
AGTGTGGATG GGCTTCTAGA TCAGGCGGGC TCAACTCCAG AGCAAAGAAT AGCAGCAGCT 
GCGGCCAAGG TGACAGCGGG TAGTACAGCT CTAACGAACG CCAGTTCCAC AAGCACTCTG 
CAGCCCGAAG AAGAGGTGAC GGATGCCGCC CTCAATGACT CGGTGACCCG GCGTGACAAG 
CATACTTTAT CCCCACCGGG AAATGTACAG CAGCACAGAA AGTCCCGAAG TCTGGATCAT 
ATTCTCAGCA AACAGACTTT AGCAGAGCTG CTTCCACCGA GTAGCGAACT CGCAGATGGA 
ACGCAAAGTA TGCAAAACCT GGCCATTCCA ATGACCCCGC AGCCCCAACG AAGGGACACC 
AGCTCCTCCT CGAAATCCCC CACTCCCGAA AGACCTCCGC AACCAGCGAT GGAAAGGGTA 
AGGGAACGCC AGAGTCCTGA GGGTGTGAGT GCGACGGAAA GCGAGCGGGA GGATCAGCCA 
GAAGAATGTC TGCGACATGG CAACCAACAG AGGGCATCTG CCTCGGTTCA TCGGGGAGCC 
AGTTTGACCA GCAACAAGAC CTCCAACTCC TCACTGGGCT CCAACTTTAG TGCCGGAGGA 
AACAACAAGA CGATATTTAA TCGAACGATG AAGAAGGTCC GCTCGCTGAT AAAAAACAAC 
GAAATGGAGG ACGAGGAACT TTCGGGCATC ATTCTGTCCA AGGCCACCTC CCCCAATGCC 
GGGCGCATGA TATTTTGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ect4 ( NM_001043129.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001036594.1 Drosophila melanogaster Ectoderm-expressed 4 (Ect4), transcript variant F, mRNA.

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CloneID	OFa17862
Clone ID Related Accession (Same CDS sequence)	NM_001043129.3
Accession Version	NM_001043129.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ectoderm-expressed 4, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001043129.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGGCGG CGGAAATCAA GCGAGATCTC ACAAACATCC AGAAGTCCAT GTCGGAAATC 
AACGATCTGG CCAAGGAGCG GATCACCGGC GGACCTGGAT CCATTAGCAC CACCTCAGCC 
TCGGCGATCA CAGCACCCAG CACGATGTCC CAGACGACGA CGAGCCGGCT GGCACCCAAA 
CTCACATCCG CCCATCCGTC AATCGACGAT CTCAGGGGTC TCAGTAGGCA GGACAAAATC 
ACCCAGCTGC AAAAAAAAAT TCGCGCCTCG TTCGAGAACC TGGTGGACCA CGATGACAGC 
AATGTGATCG TGACCCTGCC GGATGACGAC GACTGCCCGC ATAACCATTT TGGCAGCGGC 
CTCAACCTTA CCCATCCCAC CGCCGCCCAG CTGAGTGCTA GTGGGCTGAG CGGGTCGAGT 
AAGACAATCG ACACGATTAA GTTCCAGGAG AAGAGCATGA AGACCGAGTC CAAGACCAAG 
GTGGTCACCG ATGGCTTCAG TTCCGAGCAG GCCACTAGCA ATTCGGCGGA GATGAAACGT 
TTGCAGGCCG GAGATATAGA CTATCAAGAA AGCAAGGGTG CGTCGGCGAT GAGAAATCGA 
TTGGAGGTGG ATGGGGTCAA AACGGAGGAG AATGCAGCCG TCATTAAGGA AGCCCTCTCA 
CTCCGCACCG GCGACATCAC ACAGCAGGCG AGCAACAATG TGGCGGCCTC CAGTATTACG 
GTGCAAAGTG AGAATTTCTC CGCGGACAAG AAGGCGATAT CCCAGTCGCA GCAATCGCAG 
ACGATGACCT CCAACGGGAT CATCAGTCAG GAGAAACATG TATCCTCCGC CTCGCAGGCC 
AACTACTCGA TGTCGCACAA GGGCGTTTCC AGTACCGGCA GTAGCATGAT CACCAGCTCC 
TCGCAAATGT CCGCGATGAA TGGCCAGATG CTGAAGCTCG CCGATCTCAA GTTGGATGAC 
CTCAAGTCGC TGACAGCTGG CAGTGGGCAG CAGGAGATCG AGCAGACCAT CAACAAGTAT 
TCCAATATGC TGACCTCAAT AGTGAGCTCC CTGCAAGAAG ATGAGCGAGG TGGTAGTGCC 
ATAACCGTCC ACGATGTTGG TGGCAAGAAG TCTCAGTACC TGGAGAAGAT CAACGAAGTC 
ATTCGTCGAG CTTGGGCAGT GCCCACACAT GGCCACGAGC TGGGCTACTC ATTGTGCAAT 
TCCCTGCGCC AAAGTGGCGG CCTCGATCTT CTCATGAAGA ACTGCGTTAA GCCCGATCTC 
CAGTTTTCCT CCGCACAGCT TCTTGAGCAG TGCCTAACCA CCGAGAACCG CAAACATGTG 
GTGGATAATG GTCTGGATAA GGTGGTCAAT GTGGCCTGTG TCTGCACAAA GAATTCCAAT 
ATGGAGCACT CCCGCGTGGG CACTGGCATC CTGGAGCATC TGTTCAAACA CTCCGAGGGC 
ACCTGTTCGG ATGTGATAAG GCTAGGTGGT CTGGATGCCG TGCTCTTTGA GTGCCGCACC 
AGTGATTTGG AAACTCTGCG ACACTGCGCT AGTGCACTGG CAAATTTATC TCTATATGGT 
GGAGCCGAGA ATCAGGAGGA GATGATCCTA CGCAAGGTGC CCATGTGGTT GTTCCCATTG 
GCCTTCCACA ACGATGATAA CATCAAGTAC TATGCCTGCT TGGCCATTGC AGTGCTGGTA 
GCCAACAAGG AGATTGAAGC CGAGGTGCTG AAGTCTGGAT GCTTGGATTT GGTGGAACCC 
TTTGTCACCT CCCACGACCC CTCGGCTTTT GCGAGATCCA ATCTGGCACA TGCCCACGGG 
CAAAGCAAAC ATTGGCTGAA AAGATTGGTT CCAGTTTTGA GTTCCAATCG CGAAGAGGCC 
CGCAATCTGG CTGCCTTCCA TTTTTGCATG GAGGCGGGCA TCAAGAGGGA GCAGGGTAAC 
ACCGACATCT TCCGCGAGAT CAACGCTATT GAAGCTTTGA AAAATGTGGC CAGCTGTCCA 
AATGCCATTG CATCCAAGTT CGCCGCTCAG GCTCTGAGAT TGATTGGAGA GACAGTTCCG 
CACAAGCTGT CCCAACAGGT TCCCTTGTGG TCCGTGGAGG ATGTGCAGGA GTGGGTAAAG 
CAAATCGGTT TCAATGACTA CATCGACAAG TTCAACGAGT CCCAGGTGGA CGGGGATCTT 
TTACTGAAGC TCAATCAGGA TAACCTCCGG GCTGATATTG GAATCGGTAA TGGAATACTT 
CTAAAGCGTT TTGAACGCGA GCTGCAAAAT CTCAAGCGTA TGGCTGACTA CTCATCCAAG 
GACACGGCCA AGATGCACCA ATTTCTCTCG GAGATCGGTA CTGATTACTG CACCTACACG 
TATGCCATGC TGAATGCTGG AATTGACAAG TGTGCACTGC CGCATGTCAA TGAGGATATG 
CTGATGACAG AGTGTGGCAT CCACAACTCG ATCCATCGTC TGAGAATTCT CAATGCGGTC 
AAAAACTTGG AGAATTCGCT GCCCAGCTCG TCGGAGGAGA ACATGGCCAA GACGCTAGAT 
GTTTTCGTTA GCTATAGGCG ATCAAACGGC TCCCAACTAG CCAGTCTTCT CAAGGTGCAT 
CTGCAGCTTC GTGGTTTCTC CGTTTTCATC GACGTGGAGC GCCTGGAGGC GGGCAAATTT 
GACAACGGCC TATTGAACAG TATTCGACAG GCAAAGAACT TTGTCTTAGT ATTAACTCCC 
GATGCCCTGC ACCGCTGCAT TAACGACGAG GACTGCAAGG ACTGGGTGCA TCGCGAAATC 
GTGGCTGCCC TGAACTCGAA CTGCAACATT ATACCCATCA TAGACCAGCA ATTCGATTGG 
CCCGAGGTGG AGCGACTTCC CGAGGACATG CGCAGTGTGG CCCATTTTAA CGGTGTTAAT 
TGGATCCACG ACTACCAGGA CGCATGCATC GATAAGCTCG AAAGATTTTT GCGCGGCGAA 
AAGAATATCG ATCGCATTGC GGCGATGGTG CCCGGCACCC CCGGTTCGGT CTCATACCAA 
AGAATGCACA GCAACGACTC CGACTACCAG AGCGGAGGAG CAGGCGCCGG ATCAGGAGCA 
GGAACTGGAG GAGGAGGTGG TGGCGGCGTT ACGGGCAGTG TGGTGGATGG CCTGATGGTG 
GCGGCCAATG GCAGCGGACA AGCTAATCAC CAGGCAAATA GGTACCGCCA ATCTCCCTCA 
CCGGCCCGCC AAAGGGGCAG CACCTCGCAG TTGAGTGGTT ATTCTCGGGC GCCCTCGAAA 
CGCTCCCAAA TCCTCACCCC GTATCGCACC CAACAGGCAG CCCTGCTCCA CAAAACCGGA 
GCGGGTTCCG CGTCCATGCA GAATATGATG CCATTGGCGT ATCTGCCTCC GCGGAGGAGT 
TCCGCCGCGG GATTAGGTCA TGGATCTGGT TCCGGCATGG GCAGTGGCTA TCGATCGCAC 
AGTGTGGATG GGCTTCTAGA TCAGGCGGGC TCAACTCCAG AGCAAAGAAT AGCAGCAGCT 
GCGGCCAAGG TGACAGCGGG TAGTACAGCT CTAACGAACG CCAGTTCCAC AAGCACTCTG 
CAGCCCGAAG AAGAGGTGAC GGATGCCGCC CTCAATGACT CGGTGACCCG GCGTGACAAG 
CATACTTTAT CCCCACCGGG AAATGTACAG CAGCACAGAA AGTCCCGAAG TCTGGATCAT 
ATTCTCAGCA AACAGACTTT AGCAGAGCTG CTTCCACCGA GTAGCGAACT CGCAGATGGA 
ACGCAAAGTA TGCAAAACCT GGCCATTCCA ATGACCCCGC AGCCCCAACG AAGGGACACC 
AGCTCCTCCT CGAAATCCCC CACTCCCGAA AGACCTCCGC AACCAGCGAT GGAAAGGGTA 
AGGGAACGCC AGAGTCCTGA GGGTGTGAGT GCGACGGAAA GCGAGCGGGA GGATCAGCCA 
GAAGAATGTC TGCGACATGG CAACCAACAG AGGGCATCTG CCTCGGTTCA TCGGGGAGCC 
AGTTTGACCA GCAACAAGAC CTCCAACTCC TCACTGGGCT CCAACTTTAG TGCCGGAGGA 
AACAACAAGA CGATATTTAA TCGAACGATG AAGAAGGTCC GCTCGCTGAT AAAAAAACCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036594.1
CDS	775..4857
Translation	MKAAEIKRDLTNIQKSMSEINDLAKERITGGPGSISTTSASAITAPSTMSQTTTSRLAPKLTSAHPSIDDLRGLSRQDKITQLQKKIRASFENLVDHDDSNVIVTLPDDDDCPHNHFGSGLNLTHPTAAQLSASGLSGSSKTIDTIKFQEKSMKTESKTKVVTDGFSSEQATSNSAEMKRLQAGDIDYQESKGASAMRNRLEVDGVKTEENAAVIKEALSLRTGDITQQASNNVAASSITVQSENFSADKKAISQSQQSQTMTSNGIISQEKHVSSASQANYSMSHKGVSSTGSSMITSSSQMSAMNGQMLKLADLKLDDLKSLTAGSGQQEIEQTINKYSNMLTSIVSSLQEDERGGSAITVHDVGGKKSQYLEKINEVIRRAWAVPTHGHELGYSLCNSLRQSGGLDLLMKNCVKPDLQFSSAQLLEQCLTTENRKHVVDNGLDKVVNVACVCTKNSNMEHSRVGTGILEHLFKHSEGTCSDVIRLGGLDAVLFECRTSDLETLRHCASALANLSLYGGAENQEEMILRKVPMWLFPLAFHNDDNIKYYACLAIAVLVANKEIEAEVLKSGCLDLVEPFVTSHDPSAFARSNLAHAHGQSKHWLKRLVPVLSSNREEARNLAAFHFCMEAGIKREQGNTDIFREINAIEALKNVASCPNAIASKFAAQALRLIGETVPHKLSQQVPLWSVEDVQEWVKQIGFNDYIDKFNESQVDGDLLLKLNQDNLRADIGIGNGILLKRFERELQNLKRMADYSSKDTAKMHQFLSEIGTDYCTYTYAMLNAGIDKCALPHVNEDMLMTECGIHNSIHRLRILNAVKNLENSLPSSSEENMAKTLDVFVSYRRSNGSQLASLLKVHLQLRGFSVFIDVERLEAGKFDNGLLNSIRQAKNFVLVLTPDALHRCINDEDCKDWVHREIVAALNSNCNIIPIIDQQFDWPEVERLPEDMRSVAHFNGVNWIHDYQDACIDKLERFLRGEKNIDRIAAMVPGTPGSVSYQRMHSNDSDYQSGGAGAGSGAGTGGGGGGGVTGSVVDGLMVAANGSGQANHQANRYRQSPSPARQRGSTSQLSGYSRAPSKRSQILTPYRTQQAALLHKTGAGSASMQNMMPLAYLPPRRSSAAGLGHGSGSGMGSGYRSHSVDGLLDQAGSTPEQRIAAAAAKVTAGSTALTNASSTSTLQPEEEVTDAALNDSVTRRDKHTLSPPGNVQQHRKSRSLDHILSKQTLAELLPPSSELADGTQSMQNLAIPMTPQPQRRDTSSSSKSPTPERPPQPAMERVRERQSPEGVSATESEREDQPEECLRHGNQQRASASVHRGASLTSNKTSNSSLGSNFSAGGNNKTIFNRTMKKVRSLIKKP

Target ORF information:

RefSeq Version	NM_001043129.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ectoderm-expressed 4 (Ect4), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001043129.3

ORF Insert Sequence:

ATGAAGGCGG CGGAAATCAA GCGAGATCTC ACAAACATCC AGAAGTCCAT GTCGGAAATC 
AACGATCTGG CCAAGGAGCG GATCACCGGC GGACCTGGAT CCATTAGCAC CACCTCAGCC 
TCGGCGATCA CAGCACCCAG CACGATGTCC CAGACGACGA CGAGCCGGCT GGCACCCAAA 
CTCACATCCG CCCATCCGTC AATCGACGAT CTCAGGGGTC TCAGTAGGCA GGACAAAATC 
ACCCAGCTGC AAAAAAAAAT TCGCGCCTCG TTCGAGAACC TGGTGGACCA CGATGACAGC 
AATGTGATCG TGACCCTGCC GGATGACGAC GACTGCCCGC ATAACCATTT TGGCAGCGGC 
CTCAACCTTA CCCATCCCAC CGCCGCCCAG CTGAGTGCTA GTGGGCTGAG CGGGTCGAGT 
AAGACAATCG ACACGATTAA GTTCCAGGAG AAGAGCATGA AGACCGAGTC CAAGACCAAG 
GTGGTCACCG ATGGCTTCAG TTCCGAGCAG GCCACTAGCA ATTCGGCGGA GATGAAACGT 
TTGCAGGCCG GAGATATAGA CTATCAAGAA AGCAAGGGTG CGTCGGCGAT GAGAAATCGA 
TTGGAGGTGG ATGGGGTCAA AACGGAGGAG AATGCAGCCG TCATTAAGGA AGCCCTCTCA 
CTCCGCACCG GCGACATCAC ACAGCAGGCG AGCAACAATG TGGCGGCCTC CAGTATTACG 
GTGCAAAGTG AGAATTTCTC CGCGGACAAG AAGGCGATAT CCCAGTCGCA GCAATCGCAG 
ACGATGACCT CCAACGGGAT CATCAGTCAG GAGAAACATG TATCCTCCGC CTCGCAGGCC 
AACTACTCGA TGTCGCACAA GGGCGTTTCC AGTACCGGCA GTAGCATGAT CACCAGCTCC 
TCGCAAATGT CCGCGATGAA TGGCCAGATG CTGAAGCTCG CCGATCTCAA GTTGGATGAC 
CTCAAGTCGC TGACAGCTGG CAGTGGGCAG CAGGAGATCG AGCAGACCAT CAACAAGTAT 
TCCAATATGC TGACCTCAAT AGTGAGCTCC CTGCAAGAAG ATGAGCGAGG TGGTAGTGCC 
ATAACCGTCC ACGATGTTGG TGGCAAGAAG TCTCAGTACC TGGAGAAGAT CAACGAAGTC 
ATTCGTCGAG CTTGGGCAGT GCCCACACAT GGCCACGAGC TGGGCTACTC ATTGTGCAAT 
TCCCTGCGCC AAAGTGGCGG CCTCGATCTT CTCATGAAGA ACTGCGTTAA GCCCGATCTC 
CAGTTTTCCT CCGCACAGCT TCTTGAGCAG TGCCTAACCA CCGAGAACCG CAAACATGTG 
GTGGATAATG GTCTGGATAA GGTGGTCAAT GTGGCCTGTG TCTGCACAAA GAATTCCAAT 
ATGGAGCACT CCCGCGTGGG CACTGGCATC CTGGAGCATC TGTTCAAACA CTCCGAGGGC 
ACCTGTTCGG ATGTGATAAG GCTAGGTGGT CTGGATGCCG TGCTCTTTGA GTGCCGCACC 
AGTGATTTGG AAACTCTGCG ACACTGCGCT AGTGCACTGG CAAATTTATC TCTATATGGT 
GGAGCCGAGA ATCAGGAGGA GATGATCCTA CGCAAGGTGC CCATGTGGTT GTTCCCATTG 
GCCTTCCACA ACGATGATAA CATCAAGTAC TATGCCTGCT TGGCCATTGC AGTGCTGGTA 
GCCAACAAGG AGATTGAAGC CGAGGTGCTG AAGTCTGGAT GCTTGGATTT GGTGGAACCC 
TTTGTCACCT CCCACGACCC CTCGGCTTTT GCGAGATCCA ATCTGGCACA TGCCCACGGG 
CAAAGCAAAC ATTGGCTGAA AAGATTGGTT CCAGTTTTGA GTTCCAATCG CGAAGAGGCC 
CGCAATCTGG CTGCCTTCCA TTTTTGCATG GAGGCGGGCA TCAAGAGGGA GCAGGGTAAC 
ACCGACATCT TCCGCGAGAT CAACGCTATT GAAGCTTTGA AAAATGTGGC CAGCTGTCCA 
AATGCCATTG CATCCAAGTT CGCCGCTCAG GCTCTGAGAT TGATTGGAGA GACAGTTCCG 
CACAAGCTGT CCCAACAGGT TCCCTTGTGG TCCGTGGAGG ATGTGCAGGA GTGGGTAAAG 
CAAATCGGTT TCAATGACTA CATCGACAAG TTCAACGAGT CCCAGGTGGA CGGGGATCTT 
TTACTGAAGC TCAATCAGGA TAACCTCCGG GCTGATATTG GAATCGGTAA TGGAATACTT 
CTAAAGCGTT TTGAACGCGA GCTGCAAAAT CTCAAGCGTA TGGCTGACTA CTCATCCAAG 
GACACGGCCA AGATGCACCA ATTTCTCTCG GAGATCGGTA CTGATTACTG CACCTACACG 
TATGCCATGC TGAATGCTGG AATTGACAAG TGTGCACTGC CGCATGTCAA TGAGGATATG 
CTGATGACAG AGTGTGGCAT CCACAACTCG ATCCATCGTC TGAGAATTCT CAATGCGGTC 
AAAAACTTGG AGAATTCGCT GCCCAGCTCG TCGGAGGAGA ACATGGCCAA GACGCTAGAT 
GTTTTCGTTA GCTATAGGCG ATCAAACGGC TCCCAACTAG CCAGTCTTCT CAAGGTGCAT 
CTGCAGCTTC GTGGTTTCTC CGTTTTCATC GACGTGGAGC GCCTGGAGGC GGGCAAATTT 
GACAACGGCC TATTGAACAG TATTCGACAG GCAAAGAACT TTGTCTTAGT ATTAACTCCC 
GATGCCCTGC ACCGCTGCAT TAACGACGAG GACTGCAAGG ACTGGGTGCA TCGCGAAATC 
GTGGCTGCCC TGAACTCGAA CTGCAACATT ATACCCATCA TAGACCAGCA ATTCGATTGG 
CCCGAGGTGG AGCGACTTCC CGAGGACATG CGCAGTGTGG CCCATTTTAA CGGTGTTAAT 
TGGATCCACG ACTACCAGGA CGCATGCATC GATAAGCTCG AAAGATTTTT GCGCGGCGAA 
AAGAATATCG ATCGCATTGC GGCGATGGTG CCCGGCACCC CCGGTTCGGT CTCATACCAA 
AGAATGCACA GCAACGACTC CGACTACCAG AGCGGAGGAG CAGGCGCCGG ATCAGGAGCA 
GGAACTGGAG GAGGAGGTGG TGGCGGCGTT ACGGGCAGTG TGGTGGATGG CCTGATGGTG 
GCGGCCAATG GCAGCGGACA AGCTAATCAC CAGGCAAATA GGTACCGCCA ATCTCCCTCA 
CCGGCCCGCC AAAGGGGCAG CACCTCGCAG TTGAGTGGTT ATTCTCGGGC GCCCTCGAAA 
CGCTCCCAAA TCCTCACCCC GTATCGCACC CAACAGGCAG CCCTGCTCCA CAAAACCGGA 
GCGGGTTCCG CGTCCATGCA GAATATGATG CCATTGGCGT ATCTGCCTCC GCGGAGGAGT 
TCCGCCGCGG GATTAGGTCA TGGATCTGGT TCCGGCATGG GCAGTGGCTA TCGATCGCAC 
AGTGTGGATG GGCTTCTAGA TCAGGCGGGC TCAACTCCAG AGCAAAGAAT AGCAGCAGCT 
GCGGCCAAGG TGACAGCGGG TAGTACAGCT CTAACGAACG CCAGTTCCAC AAGCACTCTG 
CAGCCCGAAG AAGAGGTGAC GGATGCCGCC CTCAATGACT CGGTGACCCG GCGTGACAAG 
CATACTTTAT CCCCACCGGG AAATGTACAG CAGCACAGAA AGTCCCGAAG TCTGGATCAT 
ATTCTCAGCA AACAGACTTT AGCAGAGCTG CTTCCACCGA GTAGCGAACT CGCAGATGGA 
ACGCAAAGTA TGCAAAACCT GGCCATTCCA ATGACCCCGC AGCCCCAACG AAGGGACACC 
AGCTCCTCCT CGAAATCCCC CACTCCCGAA AGACCTCCGC AACCAGCGAT GGAAAGGGTA 
AGGGAACGCC AGAGTCCTGA GGGTGTGAGT GCGACGGAAA GCGAGCGGGA GGATCAGCCA 
GAAGAATGTC TGCGACATGG CAACCAACAG AGGGCATCTG CCTCGGTTCA TCGGGGAGCC 
AGTTTGACCA GCAACAAGAC CTCCAACTCC TCACTGGGCT CCAACTTTAG TGCCGGAGGA 
AACAACAAGA CGATATTTAA TCGAACGATG AAGAAGGTCC GCTCGCTGAT AAAAAAACCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ect4 ( NM_001259733.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246662.1 Drosophila melanogaster Ectoderm-expressed 4 (Ect4), transcript variant I, mRNA.

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CloneID	OFa20689
Clone ID Related Accession (Same CDS sequence)	NM_001259733.1
Accession Version	NM_001259733.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ectoderm-expressed 4, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACTCAC CACAGCTGGC GCCCACCACT GCGCAATCTC ACCACGCTGA GAGCGCTGAC 
AAAACAGCGC CGCCTGGAAA TAAATATGCG CCTGGAAGAA GGCTAGCTGC GCGGCAAACT 
CTTCGATTGG CAATACCGAA GGCGCAACCC GGTGACCCAC TGAATCACCC AACGAGTGTC 
GGCCTCTTTC GTCCCTCACC ATCGCAACTG CCACCGGCAC CGATTTTGAA GCGCGTTCCA 
GCGGTCAGCG GCGGGTGCAG TGGAATACCG AAGTCCGCCT CGCCACTGGA ATCGCACCAC 
CTGCCCAATG TCACGCCCAC CAACGCGCAC ACCCGTTTGG GCTATGGCAT GCAGGGCGCG 
GGGGGAGGCA GTAGCTGTTC CAGTGCCTCA TCAGCATCCA ATTTTCTGGG CGTGCGCAAG 
CCCAGCCTGA CCTTGAACTC ATCGGATATC CGGAACAACA ACGATCTGTT CGCCTTCCCC 
AAGGACATGT CGCCTGTGAT GAGTGAACAT TGCCACTCGC CTATGTTTAC CGACCTATCA 
TCAGCTAATA GCAATGGTTC ACCAACACAT CACCAGCAGC TGGGCAGGCT ATTCAGCCTC 
AGTCCTTCGA GCATCAGAAG TTACGGAAAT ATGACAGACT TCTCGGATAG CTCGCCAAGT 
GGGGTGCTGA GTCCACCCAC CTCGCTGATC GAGAATTTCC ACAAGAAGAA CAGCTCACCG 
CAGCCACTTG GCAGTAGCAG CGCCATAAGC ACCCTAACTC CAACAACCTT GAATGGATCT 
GTGGTTGGCG GTGGAGTGGG TGGTGCTGGA CACCACAGCA TCGAGAATGG CAGGCAAATC 
AATACCAGCT CTAGTTCATG CAGTAGTCTT AAGGAAACCT CACACCAGTC CACATCCACA 
TCACAACAGG TTGTCAACAG CAGCAGCAGC ACAACGACGC GGGTGGAGAA GAAGTCTCAG 
CGTTTGCACC ACCACATAAC CTCCTCATCC TCCAGCTCTT CAACCACCCA GGCTCTGTCC 
ACCTCATCAG AGATGAAGGC GGCGGAAATC AAGCGAGATC TCACAAACAT CCAGAAGTCC 
ATGTCGGAAA TCAACGATCT GGCCAAGGAG CGGATCACCG GCGGACCTGG ATCCATTAGC 
ACCACCTCAG CCTCGGCGAT CACAGCACCC AGCACGATGT CCCAGACGAC GACGAGCCGG 
CTGGCACCCA AACTCACATC CGCCCATCCG TCAATCGACG ATCTCAGGGG TCTCAGTAGG 
CAGGACAAAA TCACCCAGCT GCAAAAAAAA ATTCGCGCCT CGTTCGAGAA CCTGGTGGAC 
CACGATGACA GCAATGTGAT CGTGACCCTG CCGGATGACG ACGACTGCCC GCATAACCAT 
TTTGGCAGCG GCCTCAACCT TACCCATCCC ACCGCCGCCC AGCTGAGTGC TAGTGGGCTG 
AGCGGGTCGA GTAAGACAAT CGACACGATT AAGTTCCAGG AGAAGAGCAT GAAGACCGAG 
TCCAAGACCA AGGTGGTCAC CGATGGCTTC AGTTCCGAGC AGGCCACTAG CAATTCGGCG 
GAGATGAAAC GTTTGCAGGC CGGAGATATA GACTATCAAG AAAGCAAGGG TGCGTCGGCG 
ATGAGAAATC GATTGGAGGT GGATGGGGTC AAAACGGAGG AGAATGCAGC CGTCATTAAG 
GAAGCCCTCT CACTCCGCAC CGGCGACATC ACACAGCAGG CGAGCAACAA TGTGGCGGCC 
TCCAGTATTA CGGTGCAAAG TGAGAATTTC TCCGCGGACA AGAAGGCGAT ATCCCAGTCG 
CAGCAATCGC AGACGATGAC CTCCAACGGG ATCATCAGTC AGGAGAAACA TGTATCCTCC 
GCCTCGCAGG CCAACTACTC GATGTCGCAC AAGGGCGTTT CCAGTACCGG CAGTAGCATG 
ATCACCAGCT CCTCGCAAAT GTCCGCGATG AATGGCCAGA TGCTGAAGCT CGCCGATCTC 
AAGTTGGATG ACCTCAAGTC GCTGACAGCT GGCAGTGGGC AGCAGGAGAT CGAGCAGACC 
ATCAACAAGT ATTCCAATAT GCTGACCTCA ATAGTGAGCT CCCTGCAAGA AGATGAGCGA 
GGTGGTAGTG CCATAACCGT CCACGATGTT GGTGGCAAGA AGTCTCAGTA CCTGGAGAAG 
ATCAACGAAG TCATTCGTCG AGCTTGGGCA GTGCCCACAC ATGGCCACGA GCTGGGCTAC 
TCATTGTGCA ATTCCCTGCG CCAAAGTGGC GGCCTCGATC TTCTCATGAA GAACTGCGTT 
AAGCCCGATC TCCAGTTTTC CTCCGCACAG CTTCTTGAGC AGTGCCTAAC CACCGAGAAC 
CGCAAACATG TGGTGGATAA TGGTCTGGAT AAGGTGGTCA ATGTGGCCTG TGTCTGCACA 
AAGAATTCCA ATATGGAGCA CTCCCGCGTG GGCACTGGCA TCCTGGAGCA TCTGTTCAAA 
CACTCCGAGG GCACCTGTTC GGATGTGATA AGGCTAGGTG GTCTGGATGC CGTGCTCTTT 
GAGTGCCGCA CCAGTGATTT GGAAACTCTG CGACACTGCG CTAGTGCACT GGCAAATTTA 
TCTCTATATG GTGGAGCCGA GAATCAGGAG GAGATGATCC TACGCAAGGT GCCCATGTGG 
TTGTTCCCAT TGGCCTTCCA CAACGATGAT AACATCAAGT ACTATGCCTG CTTGGCCATT 
GCAGTGCTGG TAGCCAACAA GGAGATTGAA GCCGAGGTGC TGAAGTCTGG ATGCTTGGAT 
TTGGTGGAAC CCTTTGTCAC CTCCCACGAC CCCTCGGCTT TTGCGAGATC CAATCTGGCA 
CATGCCCACG GGCAAAGCAA ACATTGGCTG AAAAGATTGG TTCCAGTTTT GAGTTCCAAT 
CGCGAAGAGG CCCGCAATCT GGCTGCCTTC CATTTTTGCA TGGAGGCGGG CATCAAGAGG 
GAGCAGGGTA ACACCGACAT CTTCCGCGAG ATCAACGCTA TTGAAGCTTT GAAAAATGTG 
GCCAGCTGTC CAAATGCCAT TGCATCCAAG TTCGCCGCTC AGGCTCTGAG ATTGATTGGA 
GAGACAGTTC CGCACAAGCT GTCCCAACAG GTTCCCTTGT GGTCCGTGGA GGATGTGCAG 
GAGTGGGTAA AGCAAATCGG TTTCAATGAC TACATCGACA AGTTCAACGA GTCCCAGGTG 
GACGGGGATC TTTTACTGAA GCTCAATCAG GATAACCTCC GGGCTGATAT TGGAATCGGT 
AATGGAATAC TTCTAAAGCG TTTTGAACGC GAGCTGCAAA ATCTCAAGCG TATGGCTGAC 
TACTCATCCA AGGACACGGC CAAGATGCAC CAATTTCTCT CGGAGATCGG TACTGATTAC 
TGCACCTACA CGTATGCCAT GCTGAATGCT GGAATTGACA AGTGTGCACT GCCGCATGTC 
AATGAGGATA TGCTGATGAC AGAGTGTGGC ATCCACAACT CGATCCATCG TCTGAGAATT 
CTCAATGCGG TCAAAAACTT GGAGAATTCG CTGCCCAGCT CGTCGGAGGA GAACATGGCC 
AAGACGCTAG ATGTTTTCGT TAGCTATAGG CGATCAAACG GCTCCCAACT AGCCAGTCTT 
CTCAAGGTGC ATCTGCAGCT TCGTGGTTTC TCCGTTTTCA TCGACGTGGA GCGCCTGGAG 
GCGGGCAAAT TTGACAACGG CCTATTGAAC AGTATTCGAC AGGCAAAGAA CTTTGTCTTA 
GTATTAACTC CCGATGCCCT GCACCGCTGC ATTAACGACG AGGACTGCAA GGACTGGGTG 
CATCGCGAAA TCGTGGCTGC CCTGAACTCG AACTGCAACA TTATACCCAT CATAGACCAG 
CAATTCGATT GGCCCGAGGT GGAGCGACTT CCCGAGGACA TGCGCAGTGT GGCCCATTTT 
AACGGTGTTA ATTGGATCCA CGACTACCAG GACGCATGCA TCGATAAGCT CGAAAGATTT 
TTGCGCGGCG AAAAGAATAT CGATCGCATT GCGGCGATGG TGCCCGGCAC CCCCGGTTCG 
GTCTCATACC AAAGAATGCA CAGCAACGAC TCCGACTACC AGAGCGGAGG AGCAGGCGCC 
GGATCAGGAG CAGGAACTGG AGGAGGAGGT GGTGGCGGCG TTACGGGCAG TGTGGTGGAT 
GGCCTGATGG TGGCGGCCAA TGGCAGCGGA CAAGCTAATC ACCAGGCAAA TAGGTACCGC 
CAATCTCCCT CACCGGCCCG CCAAAGGGGC AGCACCTCGC AGTTGAGTGG TTATTCTCGG 
GCGCCCTCGA AACGCTCCCA AATCCTCACC CCGTATCGCA CCCAACAGGC AGCCCTGCTC 
CACAAAACCG GAGCGGGTTC CGCGTCCATG CAGAATATGA TGCCATTGGC GTATCTGCCT 
CCGCGGAGGA GTTCCGCCGC GGGATTAGGT CATGGATCTG GTTCCGGCAT GGGCAGTGGC 
TATCGATCGC ACAGTGTGGA TGGGCTTCTA GATCAGGCGG GCTCAACTCC AGAGCAAAGA 
ATAGCAGCAG CTGCGGCCAA GGTGACAGCG GGTAGTACAG CTCTAACGAA CGCCAGTTCC 
ACAAGCACTC TGCAGCCCGA AGAAGAGGTG ACGGATGCCG CCCTCAATGA CTCGGTGACC 
CGGCGTGACA AGCATACTTT ATCCCCACCG GGAAATGTAC AGCAGCACAG AAAGTCCCGA 
AGTCTGGATC ATATTCTCAG CAAACAGACT TTAGCAGAGC TGCTTCCACC GAGTAGCGAA 
CTCGCAGATG GAACGCAAAG TATGCAAAAC CTGGCCATTC CAATGACCCC GCAGCCCCAA 
CGAAGGGACA CCAGCTCCTC CTCGAAATCC CCCACTCCCG AAAGACCTCC GCAACCAGCG 
ATGGAAAGGG TAAGGGAACG CCAGAGTCCT GAGGGTGTGA GTGCGACGGA AAGCGAGCGG 
GAGGATCAGC CAGAAGAATG TCTGCGACAT GGCAACCAAC AGAGGGCATC TGCCTCGGTT 
CATCGGGGAG CCAGTTTGAC CAGCAACAAG ACCTCCAACT CCTCACTGGG CTCCAACTTT 
AGTGCCGGAG GAAACAACAA GACGATATTT AATCGAACGA TGAAGAAGGT CCGCTCGCTG 
ATAAAAAACA ACGAAATGGA GGACGAGGAA CTTTCGGGCA TCATTCTGTC CAAGGCCACC 
TCCCCCAATG CCGGGCGCAT GATATTTTGG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246662.1
CDS	1436..6628
Translation	MNSPQLAPTTAQSHHAESADKTAPPGNKYAPGRRLAARQTLRLAIPKAQPGDPLNHPTSVGLFRPSPSQLPPAPILKRVPAVSGGCSGIPKSASPLESHHLPNVTPTNAHTRLGYGMQGAGGGSSCSSASSASNFLGVRKPSLTLNSSDIRNNNDLFAFPKDMSPVMSEHCHSPMFTDLSSANSNGSPTHHQQLGRLFSLSPSSIRSYGNMTDFSDSSPSGVLSPPTSLIENFHKKNSSPQPLGSSSAISTLTPTTLNGSVVGGGVGGAGHHSIENGRQINTSSSSCSSLKETSHQSTSTSQQVVNSSSSTTTRVEKKSQRLHHHITSSSSSSSTTQALSTSSEMKAAEIKRDLTNIQKSMSEINDLAKERITGGPGSISTTSASAITAPSTMSQTTTSRLAPKLTSAHPSIDDLRGLSRQDKITQLQKKIRASFENLVDHDDSNVIVTLPDDDDCPHNHFGSGLNLTHPTAAQLSASGLSGSSKTIDTIKFQEKSMKTESKTKVVTDGFSSEQATSNSAEMKRLQAGDIDYQESKGASAMRNRLEVDGVKTEENAAVIKEALSLRTGDITQQASNNVAASSITVQSENFSADKKAISQSQQSQTMTSNGIISQEKHVSSASQANYSMSHKGVSSTGSSMITSSSQMSAMNGQMLKLADLKLDDLKSLTAGSGQQEIEQTINKYSNMLTSIVSSLQEDERGGSAITVHDVGGKKSQYLEKINEVIRRAWAVPTHGHELGYSLCNSLRQSGGLDLLMKNCVKPDLQFSSAQLLEQCLTTENRKHVVDNGLDKVVNVACVCTKNSNMEHSRVGTGILEHLFKHSEGTCSDVIRLGGLDAVLFECRTSDLETLRHCASALANLSLYGGAENQEEMILRKVPMWLFPLAFHNDDNIKYYACLAIAVLVANKEIEAEVLKSGCLDLVEPFVTSHDPSAFARSNLAHAHGQSKHWLKRLVPVLSSNREEARNLAAFHFCMEAGIKREQGNTDIFREINAIEALKNVASCPNAIASKFAAQALRLIGETVPHKLSQQVPLWSVEDVQEWVKQIGFNDYIDKFNESQVDGDLLLKLNQDNLRADIGIGNGILLKRFERELQNLKRMADYSSKDTAKMHQFLSEIGTDYCTYTYAMLNAGIDKCALPHVNEDMLMTECGIHNSIHRLRILNAVKNLENSLPSSSEENMAKTLDVFVSYRRSNGSQLASLLKVHLQLRGFSVFIDVERLEAGKFDNGLLNSIRQAKNFVLVLTPDALHRCINDEDCKDWVHREIVAALNSNCNIIPIIDQQFDWPEVERLPEDMRSVAHFNGVNWIHDYQDACIDKLERFLRGEKNIDRIAAMVPGTPGSVSYQRMHSNDSDYQSGGAGAGSGAGTGGGGGGGVTGSVVDGLMVAANGSGQANHQANRYRQSPSPARQRGSTSQLSGYSRAPSKRSQILTPYRTQQAALLHKTGAGSASMQNMMPLAYLPPRRSSAAGLGHGSGSGMGSGYRSHSVDGLLDQAGSTPEQRIAAAAAKVTAGSTALTNASSTSTLQPEEEVTDAALNDSVTRRDKHTLSPPGNVQQHRKSRSLDHILSKQTLAELLPPSSELADGTQSMQNLAIPMTPQPQRRDTSSSSKSPTPERPPQPAMERVRERQSPEGVSATESEREDQPEECLRHGNQQRASASVHRGASLTSNKTSNSSLGSNFSAGGNNKTIFNRTMKKVRSLIKNNEMEDEELSGIILSKATSPNAGRMIFW

Target ORF information:

RefSeq Version	NM_001259733.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ectoderm-expressed 4 (Ect4), transcript variant I, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259733.1

ORF Insert Sequence:

ATGAACTCAC CACAGCTGGC GCCCACCACT GCGCAATCTC ACCACGCTGA GAGCGCTGAC 
AAAACAGCGC CGCCTGGAAA TAAATATGCG CCTGGAAGAA GGCTAGCTGC GCGGCAAACT 
CTTCGATTGG CAATACCGAA GGCGCAACCC GGTGACCCAC TGAATCACCC AACGAGTGTC 
GGCCTCTTTC GTCCCTCACC ATCGCAACTG CCACCGGCAC CGATTTTGAA GCGCGTTCCA 
GCGGTCAGCG GCGGGTGCAG TGGAATACCG AAGTCCGCCT CGCCACTGGA ATCGCACCAC 
CTGCCCAATG TCACGCCCAC CAACGCGCAC ACCCGTTTGG GCTATGGCAT GCAGGGCGCG 
GGGGGAGGCA GTAGCTGTTC CAGTGCCTCA TCAGCATCCA ATTTTCTGGG CGTGCGCAAG 
CCCAGCCTGA CCTTGAACTC ATCGGATATC CGGAACAACA ACGATCTGTT CGCCTTCCCC 
AAGGACATGT CGCCTGTGAT GAGTGAACAT TGCCACTCGC CTATGTTTAC CGACCTATCA 
TCAGCTAATA GCAATGGTTC ACCAACACAT CACCAGCAGC TGGGCAGGCT ATTCAGCCTC 
AGTCCTTCGA GCATCAGAAG TTACGGAAAT ATGACAGACT TCTCGGATAG CTCGCCAAGT 
GGGGTGCTGA GTCCACCCAC CTCGCTGATC GAGAATTTCC ACAAGAAGAA CAGCTCACCG 
CAGCCACTTG GCAGTAGCAG CGCCATAAGC ACCCTAACTC CAACAACCTT GAATGGATCT 
GTGGTTGGCG GTGGAGTGGG TGGTGCTGGA CACCACAGCA TCGAGAATGG CAGGCAAATC 
AATACCAGCT CTAGTTCATG CAGTAGTCTT AAGGAAACCT CACACCAGTC CACATCCACA 
TCACAACAGG TTGTCAACAG CAGCAGCAGC ACAACGACGC GGGTGGAGAA GAAGTCTCAG 
CGTTTGCACC ACCACATAAC CTCCTCATCC TCCAGCTCTT CAACCACCCA GGCTCTGTCC 
ACCTCATCAG AGATGAAGGC GGCGGAAATC AAGCGAGATC TCACAAACAT CCAGAAGTCC 
ATGTCGGAAA TCAACGATCT GGCCAAGGAG CGGATCACCG GCGGACCTGG ATCCATTAGC 
ACCACCTCAG CCTCGGCGAT CACAGCACCC AGCACGATGT CCCAGACGAC GACGAGCCGG 
CTGGCACCCA AACTCACATC CGCCCATCCG TCAATCGACG ATCTCAGGGG TCTCAGTAGG 
CAGGACAAAA TCACCCAGCT GCAAAAAAAA ATTCGCGCCT CGTTCGAGAA CCTGGTGGAC 
CACGATGACA GCAATGTGAT CGTGACCCTG CCGGATGACG ACGACTGCCC GCATAACCAT 
TTTGGCAGCG GCCTCAACCT TACCCATCCC ACCGCCGCCC AGCTGAGTGC TAGTGGGCTG 
AGCGGGTCGA GTAAGACAAT CGACACGATT AAGTTCCAGG AGAAGAGCAT GAAGACCGAG 
TCCAAGACCA AGGTGGTCAC CGATGGCTTC AGTTCCGAGC AGGCCACTAG CAATTCGGCG 
GAGATGAAAC GTTTGCAGGC CGGAGATATA GACTATCAAG AAAGCAAGGG TGCGTCGGCG 
ATGAGAAATC GATTGGAGGT GGATGGGGTC AAAACGGAGG AGAATGCAGC CGTCATTAAG 
GAAGCCCTCT CACTCCGCAC CGGCGACATC ACACAGCAGG CGAGCAACAA TGTGGCGGCC 
TCCAGTATTA CGGTGCAAAG TGAGAATTTC TCCGCGGACA AGAAGGCGAT ATCCCAGTCG 
CAGCAATCGC AGACGATGAC CTCCAACGGG ATCATCAGTC AGGAGAAACA TGTATCCTCC 
GCCTCGCAGG CCAACTACTC GATGTCGCAC AAGGGCGTTT CCAGTACCGG CAGTAGCATG 
ATCACCAGCT CCTCGCAAAT GTCCGCGATG AATGGCCAGA TGCTGAAGCT CGCCGATCTC 
AAGTTGGATG ACCTCAAGTC GCTGACAGCT GGCAGTGGGC AGCAGGAGAT CGAGCAGACC 
ATCAACAAGT ATTCCAATAT GCTGACCTCA ATAGTGAGCT CCCTGCAAGA AGATGAGCGA 
GGTGGTAGTG CCATAACCGT CCACGATGTT GGTGGCAAGA AGTCTCAGTA CCTGGAGAAG 
ATCAACGAAG TCATTCGTCG AGCTTGGGCA GTGCCCACAC ATGGCCACGA GCTGGGCTAC 
TCATTGTGCA ATTCCCTGCG CCAAAGTGGC GGCCTCGATC TTCTCATGAA GAACTGCGTT 
AAGCCCGATC TCCAGTTTTC CTCCGCACAG CTTCTTGAGC AGTGCCTAAC CACCGAGAAC 
CGCAAACATG TGGTGGATAA TGGTCTGGAT AAGGTGGTCA ATGTGGCCTG TGTCTGCACA 
AAGAATTCCA ATATGGAGCA CTCCCGCGTG GGCACTGGCA TCCTGGAGCA TCTGTTCAAA 
CACTCCGAGG GCACCTGTTC GGATGTGATA AGGCTAGGTG GTCTGGATGC CGTGCTCTTT 
GAGTGCCGCA CCAGTGATTT GGAAACTCTG CGACACTGCG CTAGTGCACT GGCAAATTTA 
TCTCTATATG GTGGAGCCGA GAATCAGGAG GAGATGATCC TACGCAAGGT GCCCATGTGG 
TTGTTCCCAT TGGCCTTCCA CAACGATGAT AACATCAAGT ACTATGCCTG CTTGGCCATT 
GCAGTGCTGG TAGCCAACAA GGAGATTGAA GCCGAGGTGC TGAAGTCTGG ATGCTTGGAT 
TTGGTGGAAC CCTTTGTCAC CTCCCACGAC CCCTCGGCTT TTGCGAGATC CAATCTGGCA 
CATGCCCACG GGCAAAGCAA ACATTGGCTG AAAAGATTGG TTCCAGTTTT GAGTTCCAAT 
CGCGAAGAGG CCCGCAATCT GGCTGCCTTC CATTTTTGCA TGGAGGCGGG CATCAAGAGG 
GAGCAGGGTA ACACCGACAT CTTCCGCGAG ATCAACGCTA TTGAAGCTTT GAAAAATGTG 
GCCAGCTGTC CAAATGCCAT TGCATCCAAG TTCGCCGCTC AGGCTCTGAG ATTGATTGGA 
GAGACAGTTC CGCACAAGCT GTCCCAACAG GTTCCCTTGT GGTCCGTGGA GGATGTGCAG 
GAGTGGGTAA AGCAAATCGG TTTCAATGAC TACATCGACA AGTTCAACGA GTCCCAGGTG 
GACGGGGATC TTTTACTGAA GCTCAATCAG GATAACCTCC GGGCTGATAT TGGAATCGGT 
AATGGAATAC TTCTAAAGCG TTTTGAACGC GAGCTGCAAA ATCTCAAGCG TATGGCTGAC 
TACTCATCCA AGGACACGGC CAAGATGCAC CAATTTCTCT CGGAGATCGG TACTGATTAC 
TGCACCTACA CGTATGCCAT GCTGAATGCT GGAATTGACA AGTGTGCACT GCCGCATGTC 
AATGAGGATA TGCTGATGAC AGAGTGTGGC ATCCACAACT CGATCCATCG TCTGAGAATT 
CTCAATGCGG TCAAAAACTT GGAGAATTCG CTGCCCAGCT CGTCGGAGGA GAACATGGCC 
AAGACGCTAG ATGTTTTCGT TAGCTATAGG CGATCAAACG GCTCCCAACT AGCCAGTCTT 
CTCAAGGTGC ATCTGCAGCT TCGTGGTTTC TCCGTTTTCA TCGACGTGGA GCGCCTGGAG 
GCGGGCAAAT TTGACAACGG CCTATTGAAC AGTATTCGAC AGGCAAAGAA CTTTGTCTTA 
GTATTAACTC CCGATGCCCT GCACCGCTGC ATTAACGACG AGGACTGCAA GGACTGGGTG 
CATCGCGAAA TCGTGGCTGC CCTGAACTCG AACTGCAACA TTATACCCAT CATAGACCAG 
CAATTCGATT GGCCCGAGGT GGAGCGACTT CCCGAGGACA TGCGCAGTGT GGCCCATTTT 
AACGGTGTTA ATTGGATCCA CGACTACCAG GACGCATGCA TCGATAAGCT CGAAAGATTT 
TTGCGCGGCG AAAAGAATAT CGATCGCATT GCGGCGATGG TGCCCGGCAC CCCCGGTTCG 
GTCTCATACC AAAGAATGCA CAGCAACGAC TCCGACTACC AGAGCGGAGG AGCAGGCGCC 
GGATCAGGAG CAGGAACTGG AGGAGGAGGT GGTGGCGGCG TTACGGGCAG TGTGGTGGAT 
GGCCTGATGG TGGCGGCCAA TGGCAGCGGA CAAGCTAATC ACCAGGCAAA TAGGTACCGC 
CAATCTCCCT CACCGGCCCG CCAAAGGGGC AGCACCTCGC AGTTGAGTGG TTATTCTCGG 
GCGCCCTCGA AACGCTCCCA AATCCTCACC CCGTATCGCA CCCAACAGGC AGCCCTGCTC 
CACAAAACCG GAGCGGGTTC CGCGTCCATG CAGAATATGA TGCCATTGGC GTATCTGCCT 
CCGCGGAGGA GTTCCGCCGC GGGATTAGGT CATGGATCTG GTTCCGGCAT GGGCAGTGGC 
TATCGATCGC ACAGTGTGGA TGGGCTTCTA GATCAGGCGG GCTCAACTCC AGAGCAAAGA 
ATAGCAGCAG CTGCGGCCAA GGTGACAGCG GGTAGTACAG CTCTAACGAA CGCCAGTTCC 
ACAAGCACTC TGCAGCCCGA AGAAGAGGTG ACGGATGCCG CCCTCAATGA CTCGGTGACC 
CGGCGTGACA AGCATACTTT ATCCCCACCG GGAAATGTAC AGCAGCACAG AAAGTCCCGA 
AGTCTGGATC ATATTCTCAG CAAACAGACT TTAGCAGAGC TGCTTCCACC GAGTAGCGAA 
CTCGCAGATG GAACGCAAAG TATGCAAAAC CTGGCCATTC CAATGACCCC GCAGCCCCAA 
CGAAGGGACA CCAGCTCCTC CTCGAAATCC CCCACTCCCG AAAGACCTCC GCAACCAGCG 
ATGGAAAGGG TAAGGGAACG CCAGAGTCCT GAGGGTGTGA GTGCGACGGA AAGCGAGCGG 
GAGGATCAGC CAGAAGAATG TCTGCGACAT GGCAACCAAC AGAGGGCATC TGCCTCGGTT 
CATCGGGGAG CCAGTTTGAC CAGCAACAAG ACCTCCAACT CCTCACTGGG CTCCAACTTT 
AGTGCCGGAG GAAACAACAA GACGATATTT AATCGAACGA TGAAGAAGGT CCGCTCGCTG 
ATAAAAAACA ACGAAATGGA GGACGAGGAA CTTTCGGGCA TCATTCTGTC CAAGGCCACC 
TCCCCCAATG CCGGGCGCAT GATATTTTGG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ect4 ( NM_001274627.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261556.1 Drosophila melanogaster Ectoderm-expressed 4 (Ect4), transcript variant K, mRNA.

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CloneID	OFa20690
Clone ID Related Accession (Same CDS sequence)	NM_001274627.1
Accession Version	NM_001274627.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ectoderm-expressed 4, isoform K
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACTCAC CACAGCTGGC GCCCACCACT GCGCAATCTC ACCACGCTGA GAGCGCTGAC 
AAAACAGCGC CGCCTGGAAA TAAATATGCG CCTGGAAGAA GGCTAGCTGC GCGGCAAACT 
CTTCGATTGG CAATACCGAA GGCGCAACCC GGTGACCCAC TGAATCACCC AACGAGTGTC 
GGCCTCTTTC GTCCCTCACC ATCGCAACTG CCACCGGCAC CGATTTTGAA GCGCGTTCCA 
GCGGTCAGCG GCGGGTGCAG TGGAATACCG AAGTCCGCCT CGCCACTGGA ATCGCACCAC 
CTGCCCAATG TCACGCCCAC CAACGCGCAC ACCCGTTTGG GCTATGGCAT GCAGGGCGCG 
GGGGGAGGCA GTAGCTGTTC CAGTGCCTCA TCAGCATCCA ATTTTCTGGG CGTGCGCAAG 
CCCAGCCTGA CCTTGAACTC ATCGGATATC CGGAACAACA ACGATCTGTT CGCCTTCCCC 
AAGGACATGT CGCCTGTGAT GAGTGAACAT TGCCACTCGC CTATGTTTAC CGACCTATCA 
TCAGCTAATA GCAATGGTTC ACCAACACAT CACCAGCAGC TGGGCAGGCT ATTCAGCCTC 
AGTCCTTCGA GCATCAGAAG TTACGGAAAT ATGACAGACT TCTCGGATAG CTCGCCAAGT 
GGGGTGCTGA GTCCACCCAC CTCGCTGATC GAGAATTTCC ACAAGAAGAA CAGCTCACCG 
CAGCCACTTG GCAGTAGCAG CGCCATAAGC ACCCTAACTC CAACAACCTT GAATGGATCT 
GTGGTTGGCG GTGGAGTGGG TGGTGCTGGA CACCACAGCA TCGAGAATGG CAGGCAAATC 
AATACCAGCT CTAGTTCATG CAGTAGTCTT AAGGAAACCT CACACCAGTC CACATCCACA 
TCACAACAGG TTGTCAACAG CAGCAGCAGC ACAACGACGC GGGTGGAGAA GAAGTCTCAG 
CGTTTGCACC ACCACATAAC CTCCTCATCC TCCAGCTCTT CAACCACCCA GGCTCTGTCC 
ACCTCATCAG AGATGAAGGC GGCGGAAATC AAGCGAGATC TCACAAACAT CCAGAAGTCC 
ATGTCGGAAA TCAACGATCT GGCCAAGGAG CGGATCACCG GCGGACCTGG ATCCATTAGC 
ACCACCTCAG CCTCGGCGAT CACAGCACCC AGCACGATGT CCCAGACGAC GACGAGCCGG 
CTGGCACCCA AACTCACATC CGCCCATCCG TCAATCGACG ATCTCAGGGG TCTCAGTAGG 
CAGGACAAAA TCACCCAGCT GCAAAAAAAA ATTCGCGCCT CGTTCGAGAA CCTGGTGGAC 
CACGATGACA GCAATGTGAT CGTGACCCTG CCGGATGACG ACGACTGCCC GCATAACCAT 
TTTGGCAGCG GCCTCAACCT TACCCATCCC ACCGCCGCCC AGCTGAGTGC TAGTGGGCTG 
AGCGGGTCGA GTAAGACAAT CGACACGATT AAGTTCCAGG AGAAGAGCAT GAAGACCGAG 
TCCAAGACCA AGGTGGTCAC CGATGGCTTC AGTTCCGAGC AGGCCACTAG CAATTCGGCG 
GAGATGAAAC GTTTGCAGGC CGGAGATATA GACTATCAAG AAAGCAAGGG TGCGTCGGCG 
ATGAGAAATC GATTGGAGGT GGATGGGGTC AAAACGGAGG AGAATGCAGC CGTCATTAAG 
GAAGCCCTCT CACTCCGCAC CGGCGACATC ACACAGCAGG CGAGCAACAA TGTGGCGGCC 
TCCAGTATTA CGGTGCAAAG TGAGAATTTC TCCGCGGACA AGAAGGCGAT ATCCCAGTCG 
CAGCAATCGC AGACGATGAC CTCCAACGGG ATCATCAGTC AGGAGAAACA TGTATCCTCC 
GCCTCGCAGG CCAACTACTC GATGTCGCAC AAGGGCGTTT CCAGTACCGG CAGTAGCATG 
ATCACCAGCT CCTCGCAAAT GTCCGCGATG AATGGCCAGA TGCTGAAGCT CGCCGATCTC 
AAGTTGGATG ACCTCAAGTC GCTGACAGCT GGCAGTGGGC AGCAGGAGAT CGAGCAGACC 
ATCAACAAGT ATTCCAATAT GCTGACCTCA ATAGTGAGCT CCCTGCAAGA AGATGAGCGA 
GGTGGTAGTG CCATAACCGT CCACGATGTT GGTGGCAAGA AGTCTCAGTA CCTGGAGAAG 
ATCAACGAAG TCATTCGTCG AGCTTGGGCA GTGCCCACAC ATGGCCACGA GCTGGGCTAC 
TCATTGTGCA ATTCCCTGCG CCAAAGTGGC GGCCTCGATC TTCTCATGAA GAACTGCGTT 
AAGCCCGATC TCCAGTTTTC CTCCGCACAG CTTCTTGAGC AGTGCCTAAC CACCGAGAAC 
CGCAAACATG TGGTGGATAA TGGTCTGGAT AAGGTGGTCA ATGTGGCCTG TGTCTGCACA 
AAGAATTCCA ATATGGAGCA CTCCCGCGTG GGCACTGGCA TCCTGGAGCA TCTGTTCAAA 
CACTCCGAGG GCACCTGTTC GGATGTGATA AGGCTAGGTG GTCTGGATGC CGTGCTCTTT 
GAGTGCCGCA CCAGTGATTT GGAAACTCTG CGACACTGCG CTAGTGCACT GGCAAATTTA 
TCTCTATATG GTGGAGCCGA GAATCAGGAG GAGATGATCC TACGCAAGGT GCCCATGTGG 
TTGTTCCCAT TGGCCTTCCA CAACGATGAT AACATCAAGT ACTATGCCTG CTTGGCCATT 
GCAGTGCTGG TAGCCAACAA GGAGATTGAA GCCGAGGTGC TGAAGTCTGG ATGCTTGGAT 
TTGGTGGAAC CCTTTGTCAC CTCCCACGAC CCCTCGGCTT TTGCGAGATC CAATCTGGCA 
CATGCCCACG GGCAAAGCAA ACATTGGCTG AAAAGATTGG TTCCAGTTTT GAGTTCCAAT 
CGCGAAGAGG CCCGCAATCT GGCTGCCTTC CATTTTTGCA TGGAGGCGGG CATCAAGAGG 
GAGCAGGGTA ACACCGACAT CTTCCGCGAG ATCAACGCTA TTGAAGCTTT GAAAAATGTG 
GCCAGCTGTC CAAATGCCAT TGCATCCAAG TTCGCCGCTC AGGCTCTGAG ATTGATTGGA 
GAGACAGTTC CGCACAAGCT GTCCCAACAG GTTCCCTTGT GGTCCGTGGA GGATGTGCAG 
GAGTGGGTAA AGCAAATCGG TTTCAATGAC TACATCGACA AGTTCAACGA GTCCCAGGTG 
GACGGGGATC TTTTACTGAA GCTCAATCAG GATAACCTCC GGGCTGATAT TGGAATCGGT 
AATGGAATAC TTCTAAAGCG TTTTGAACGC GAGCTGCAAA ATCTCAAGCG TATGGCTGAC 
TACTCATCCA AGGACACGGC CAAGATGCAC CAATTTCTCT CGGAGATCGG TACTGATTAC 
TGCACCTACA CGTATGCCAT GCTGAATGCT GGAATTGACA AGTGTGCACT GCCGCATGTC 
AATGAGGATA TGCTGATGAC AGAGTGTGGC ATCCACAACT CGATCCATCG TCTGAGAATT 
CTCAATGCGG TCAAAAACTT GGAGAATTCG CTGCCCAGCT CGTCGGAGGA GAACATGGCC 
AAGACGCTAG ATGTTTTCGT TAGCTATAGG CGATCAAACG GCTCCCAACT AGCCAGTCTT 
CTCAAGGTGC ATCTGCAGCT TCGTGGTTTC TCCGTTTTCA TCGACGTGGA GCGCCTGGAG 
GCGGGCAAAT TTGACAACGG CCTATTGAAC AGTATTCGAC AGGCAAAGAA CTTTGTCTTA 
GTATTAACTC CCGATGCCCT GCACCGCTGC ATTAACGACG AGGACTGCAA GGACTGGGTG 
CATCGCGAAA TCGTGGCTGC CCTGAACTCG AACTGCAACA TTATACCCAT CATAGACCAG 
CAATTCGATT GGCCCGAGGT GGAGCGACTT CCCGAGGACA TGCGCAGTGT GGCCCATTTT 
AACGGTGTTA ATTGGATCCA CGACTACCAG GACGCATGCA TCGATAAGCT CGAAAGATTT 
TTGCGCGGCG AAAAGAATAT CGATCGCATT GCGGCGATGG TGCCCGGCAC CCCCGGTTCG 
GTCTCATACC AAAGAATGCA CAGCAACGAC TCCGACTACC AGAGCGGAGG AGCAGGCGCC 
GGATCAGGAG CAGGAACTGG AGGAGGAGGT GGTGGCGGCG TTACGGGCAG TGTGGTGGAT 
GGCCTGATGG TGGCGGCCAA TGGCAGCGGA CAAGCTAATC ACCAGGCAAA TAGGTACCGC 
CAATCTCCCT CACCGGCCCG CCAAAGGGGC AGCACCTCGC AGTTGAGTGG TTATTCTCGG 
GCGCCCTCGA AACGCTCCCA AATCCTCACC CCGTATCGCA CCCAACAGGC AGCCCTGCTC 
CACAAAACCG GAGCGGGTTC CGCGTCCATG CAGAATATGA TGCCATTGGC GTATCTGCCT 
CCGCGGAGGA GTTCCGCCGC GGGATTAGGT CATGGATCTG GTTCCGGCAT GGGCAGTGGC 
TATCGATCGC ACAGTGTGGA TGGGCTTCTA GATCAGGCGG GCTCAACTCC AGAGCAAAGA 
ATAGCAGCAG CTGCGGCCAA GGTGACAGCG GGTAGTACAG CTCTAACGAA CGCCAGTTCC 
ACAAGCACTC TGCAGCCCGA AGAAGAGGTG ACGGATGCCG CCCTCAATGA CTCGGTGACC 
CGGCGTGACA AGCATACTTT ATCCCCACCG GGAAATGTAC AGCAGCACAG AAAGTCCCGA 
AGTCTGGATC ATATTCTCAG CAAACAGACT TTAGCAGAGC TGCTTCCACC GAGTAGCGAA 
CTCGCAGATG GAACGCAAAG TATGCAAAAC CTGGCCATTC CAATGACCCC GCAGCCCCAA 
CGAAGGGACA CCAGCTCCTC CTCGAAATCC CCCACTCCCG AAAGACCTCC GCAACCAGCG 
ATGGAAAGGG TAAGGGAACG CCAGAGTCCT GAGGGTGTGA GTGCGACGGA AAGCGAGCGG 
GAGGATCAGC CAGAAGAATG TCTGCGACAT GGCAACCAAC AGAGGGCATC TGCCTCGGTT 
CATCGGGGAG CCAGTTTGAC CAGCAACAAG ACCTCCAACT CCTCACTGGG CTCCAACTTT 
AGTGCCGGAG GAAACAACAA GACGATATTT AATCGAACGA TGAAGAAGGT CCGCTCGCTG 
ATAAAAAAAC CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261556.1
CDS	849..5963
Translation	MNSPQLAPTTAQSHHAESADKTAPPGNKYAPGRRLAARQTLRLAIPKAQPGDPLNHPTSVGLFRPSPSQLPPAPILKRVPAVSGGCSGIPKSASPLESHHLPNVTPTNAHTRLGYGMQGAGGGSSCSSASSASNFLGVRKPSLTLNSSDIRNNNDLFAFPKDMSPVMSEHCHSPMFTDLSSANSNGSPTHHQQLGRLFSLSPSSIRSYGNMTDFSDSSPSGVLSPPTSLIENFHKKNSSPQPLGSSSAISTLTPTTLNGSVVGGGVGGAGHHSIENGRQINTSSSSCSSLKETSHQSTSTSQQVVNSSSSTTTRVEKKSQRLHHHITSSSSSSSTTQALSTSSEMKAAEIKRDLTNIQKSMSEINDLAKERITGGPGSISTTSASAITAPSTMSQTTTSRLAPKLTSAHPSIDDLRGLSRQDKITQLQKKIRASFENLVDHDDSNVIVTLPDDDDCPHNHFGSGLNLTHPTAAQLSASGLSGSSKTIDTIKFQEKSMKTESKTKVVTDGFSSEQATSNSAEMKRLQAGDIDYQESKGASAMRNRLEVDGVKTEENAAVIKEALSLRTGDITQQASNNVAASSITVQSENFSADKKAISQSQQSQTMTSNGIISQEKHVSSASQANYSMSHKGVSSTGSSMITSSSQMSAMNGQMLKLADLKLDDLKSLTAGSGQQEIEQTINKYSNMLTSIVSSLQEDERGGSAITVHDVGGKKSQYLEKINEVIRRAWAVPTHGHELGYSLCNSLRQSGGLDLLMKNCVKPDLQFSSAQLLEQCLTTENRKHVVDNGLDKVVNVACVCTKNSNMEHSRVGTGILEHLFKHSEGTCSDVIRLGGLDAVLFECRTSDLETLRHCASALANLSLYGGAENQEEMILRKVPMWLFPLAFHNDDNIKYYACLAIAVLVANKEIEAEVLKSGCLDLVEPFVTSHDPSAFARSNLAHAHGQSKHWLKRLVPVLSSNREEARNLAAFHFCMEAGIKREQGNTDIFREINAIEALKNVASCPNAIASKFAAQALRLIGETVPHKLSQQVPLWSVEDVQEWVKQIGFNDYIDKFNESQVDGDLLLKLNQDNLRADIGIGNGILLKRFERELQNLKRMADYSSKDTAKMHQFLSEIGTDYCTYTYAMLNAGIDKCALPHVNEDMLMTECGIHNSIHRLRILNAVKNLENSLPSSSEENMAKTLDVFVSYRRSNGSQLASLLKVHLQLRGFSVFIDVERLEAGKFDNGLLNSIRQAKNFVLVLTPDALHRCINDEDCKDWVHREIVAALNSNCNIIPIIDQQFDWPEVERLPEDMRSVAHFNGVNWIHDYQDACIDKLERFLRGEKNIDRIAAMVPGTPGSVSYQRMHSNDSDYQSGGAGAGSGAGTGGGGGGGVTGSVVDGLMVAANGSGQANHQANRYRQSPSPARQRGSTSQLSGYSRAPSKRSQILTPYRTQQAALLHKTGAGSASMQNMMPLAYLPPRRSSAAGLGHGSGSGMGSGYRSHSVDGLLDQAGSTPEQRIAAAAAKVTAGSTALTNASSTSTLQPEEEVTDAALNDSVTRRDKHTLSPPGNVQQHRKSRSLDHILSKQTLAELLPPSSELADGTQSMQNLAIPMTPQPQRRDTSSSSKSPTPERPPQPAMERVRERQSPEGVSATESEREDQPEECLRHGNQQRASASVHRGASLTSNKTSNSSLGSNFSAGGNNKTIFNRTMKKVRSLIKKP

Target ORF information:

RefSeq Version	NM_001274627.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ectoderm-expressed 4 (Ect4), transcript variant K, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274627.1

ORF Insert Sequence:

ATGAACTCAC CACAGCTGGC GCCCACCACT GCGCAATCTC ACCACGCTGA GAGCGCTGAC 
AAAACAGCGC CGCCTGGAAA TAAATATGCG CCTGGAAGAA GGCTAGCTGC GCGGCAAACT 
CTTCGATTGG CAATACCGAA GGCGCAACCC GGTGACCCAC TGAATCACCC AACGAGTGTC 
GGCCTCTTTC GTCCCTCACC ATCGCAACTG CCACCGGCAC CGATTTTGAA GCGCGTTCCA 
GCGGTCAGCG GCGGGTGCAG TGGAATACCG AAGTCCGCCT CGCCACTGGA ATCGCACCAC 
CTGCCCAATG TCACGCCCAC CAACGCGCAC ACCCGTTTGG GCTATGGCAT GCAGGGCGCG 
GGGGGAGGCA GTAGCTGTTC CAGTGCCTCA TCAGCATCCA ATTTTCTGGG CGTGCGCAAG 
CCCAGCCTGA CCTTGAACTC ATCGGATATC CGGAACAACA ACGATCTGTT CGCCTTCCCC 
AAGGACATGT CGCCTGTGAT GAGTGAACAT TGCCACTCGC CTATGTTTAC CGACCTATCA 
TCAGCTAATA GCAATGGTTC ACCAACACAT CACCAGCAGC TGGGCAGGCT ATTCAGCCTC 
AGTCCTTCGA GCATCAGAAG TTACGGAAAT ATGACAGACT TCTCGGATAG CTCGCCAAGT 
GGGGTGCTGA GTCCACCCAC CTCGCTGATC GAGAATTTCC ACAAGAAGAA CAGCTCACCG 
CAGCCACTTG GCAGTAGCAG CGCCATAAGC ACCCTAACTC CAACAACCTT GAATGGATCT 
GTGGTTGGCG GTGGAGTGGG TGGTGCTGGA CACCACAGCA TCGAGAATGG CAGGCAAATC 
AATACCAGCT CTAGTTCATG CAGTAGTCTT AAGGAAACCT CACACCAGTC CACATCCACA 
TCACAACAGG TTGTCAACAG CAGCAGCAGC ACAACGACGC GGGTGGAGAA GAAGTCTCAG 
CGTTTGCACC ACCACATAAC CTCCTCATCC TCCAGCTCTT CAACCACCCA GGCTCTGTCC 
ACCTCATCAG AGATGAAGGC GGCGGAAATC AAGCGAGATC TCACAAACAT CCAGAAGTCC 
ATGTCGGAAA TCAACGATCT GGCCAAGGAG CGGATCACCG GCGGACCTGG ATCCATTAGC 
ACCACCTCAG CCTCGGCGAT CACAGCACCC AGCACGATGT CCCAGACGAC GACGAGCCGG 
CTGGCACCCA AACTCACATC CGCCCATCCG TCAATCGACG ATCTCAGGGG TCTCAGTAGG 
CAGGACAAAA TCACCCAGCT GCAAAAAAAA ATTCGCGCCT CGTTCGAGAA CCTGGTGGAC 
CACGATGACA GCAATGTGAT CGTGACCCTG CCGGATGACG ACGACTGCCC GCATAACCAT 
TTTGGCAGCG GCCTCAACCT TACCCATCCC ACCGCCGCCC AGCTGAGTGC TAGTGGGCTG 
AGCGGGTCGA GTAAGACAAT CGACACGATT AAGTTCCAGG AGAAGAGCAT GAAGACCGAG 
TCCAAGACCA AGGTGGTCAC CGATGGCTTC AGTTCCGAGC AGGCCACTAG CAATTCGGCG 
GAGATGAAAC GTTTGCAGGC CGGAGATATA GACTATCAAG AAAGCAAGGG TGCGTCGGCG 
ATGAGAAATC GATTGGAGGT GGATGGGGTC AAAACGGAGG AGAATGCAGC CGTCATTAAG 
GAAGCCCTCT CACTCCGCAC CGGCGACATC ACACAGCAGG CGAGCAACAA TGTGGCGGCC 
TCCAGTATTA CGGTGCAAAG TGAGAATTTC TCCGCGGACA AGAAGGCGAT ATCCCAGTCG 
CAGCAATCGC AGACGATGAC CTCCAACGGG ATCATCAGTC AGGAGAAACA TGTATCCTCC 
GCCTCGCAGG CCAACTACTC GATGTCGCAC AAGGGCGTTT CCAGTACCGG CAGTAGCATG 
ATCACCAGCT CCTCGCAAAT GTCCGCGATG AATGGCCAGA TGCTGAAGCT CGCCGATCTC 
AAGTTGGATG ACCTCAAGTC GCTGACAGCT GGCAGTGGGC AGCAGGAGAT CGAGCAGACC 
ATCAACAAGT ATTCCAATAT GCTGACCTCA ATAGTGAGCT CCCTGCAAGA AGATGAGCGA 
GGTGGTAGTG CCATAACCGT CCACGATGTT GGTGGCAAGA AGTCTCAGTA CCTGGAGAAG 
ATCAACGAAG TCATTCGTCG AGCTTGGGCA GTGCCCACAC ATGGCCACGA GCTGGGCTAC 
TCATTGTGCA ATTCCCTGCG CCAAAGTGGC GGCCTCGATC TTCTCATGAA GAACTGCGTT 
AAGCCCGATC TCCAGTTTTC CTCCGCACAG CTTCTTGAGC AGTGCCTAAC CACCGAGAAC 
CGCAAACATG TGGTGGATAA TGGTCTGGAT AAGGTGGTCA ATGTGGCCTG TGTCTGCACA 
AAGAATTCCA ATATGGAGCA CTCCCGCGTG GGCACTGGCA TCCTGGAGCA TCTGTTCAAA 
CACTCCGAGG GCACCTGTTC GGATGTGATA AGGCTAGGTG GTCTGGATGC CGTGCTCTTT 
GAGTGCCGCA CCAGTGATTT GGAAACTCTG CGACACTGCG CTAGTGCACT GGCAAATTTA 
TCTCTATATG GTGGAGCCGA GAATCAGGAG GAGATGATCC TACGCAAGGT GCCCATGTGG 
TTGTTCCCAT TGGCCTTCCA CAACGATGAT AACATCAAGT ACTATGCCTG CTTGGCCATT 
GCAGTGCTGG TAGCCAACAA GGAGATTGAA GCCGAGGTGC TGAAGTCTGG ATGCTTGGAT 
TTGGTGGAAC CCTTTGTCAC CTCCCACGAC CCCTCGGCTT TTGCGAGATC CAATCTGGCA 
CATGCCCACG GGCAAAGCAA ACATTGGCTG AAAAGATTGG TTCCAGTTTT GAGTTCCAAT 
CGCGAAGAGG CCCGCAATCT GGCTGCCTTC CATTTTTGCA TGGAGGCGGG CATCAAGAGG 
GAGCAGGGTA ACACCGACAT CTTCCGCGAG ATCAACGCTA TTGAAGCTTT GAAAAATGTG 
GCCAGCTGTC CAAATGCCAT TGCATCCAAG TTCGCCGCTC AGGCTCTGAG ATTGATTGGA 
GAGACAGTTC CGCACAAGCT GTCCCAACAG GTTCCCTTGT GGTCCGTGGA GGATGTGCAG 
GAGTGGGTAA AGCAAATCGG TTTCAATGAC TACATCGACA AGTTCAACGA GTCCCAGGTG 
GACGGGGATC TTTTACTGAA GCTCAATCAG GATAACCTCC GGGCTGATAT TGGAATCGGT 
AATGGAATAC TTCTAAAGCG TTTTGAACGC GAGCTGCAAA ATCTCAAGCG TATGGCTGAC 
TACTCATCCA AGGACACGGC CAAGATGCAC CAATTTCTCT CGGAGATCGG TACTGATTAC 
TGCACCTACA CGTATGCCAT GCTGAATGCT GGAATTGACA AGTGTGCACT GCCGCATGTC 
AATGAGGATA TGCTGATGAC AGAGTGTGGC ATCCACAACT CGATCCATCG TCTGAGAATT 
CTCAATGCGG TCAAAAACTT GGAGAATTCG CTGCCCAGCT CGTCGGAGGA GAACATGGCC 
AAGACGCTAG ATGTTTTCGT TAGCTATAGG CGATCAAACG GCTCCCAACT AGCCAGTCTT 
CTCAAGGTGC ATCTGCAGCT TCGTGGTTTC TCCGTTTTCA TCGACGTGGA GCGCCTGGAG 
GCGGGCAAAT TTGACAACGG CCTATTGAAC AGTATTCGAC AGGCAAAGAA CTTTGTCTTA 
GTATTAACTC CCGATGCCCT GCACCGCTGC ATTAACGACG AGGACTGCAA GGACTGGGTG 
CATCGCGAAA TCGTGGCTGC CCTGAACTCG AACTGCAACA TTATACCCAT CATAGACCAG 
CAATTCGATT GGCCCGAGGT GGAGCGACTT CCCGAGGACA TGCGCAGTGT GGCCCATTTT 
AACGGTGTTA ATTGGATCCA CGACTACCAG GACGCATGCA TCGATAAGCT CGAAAGATTT 
TTGCGCGGCG AAAAGAATAT CGATCGCATT GCGGCGATGG TGCCCGGCAC CCCCGGTTCG 
GTCTCATACC AAAGAATGCA CAGCAACGAC TCCGACTACC AGAGCGGAGG AGCAGGCGCC 
GGATCAGGAG CAGGAACTGG AGGAGGAGGT GGTGGCGGCG TTACGGGCAG TGTGGTGGAT 
GGCCTGATGG TGGCGGCCAA TGGCAGCGGA CAAGCTAATC ACCAGGCAAA TAGGTACCGC 
CAATCTCCCT CACCGGCCCG CCAAAGGGGC AGCACCTCGC AGTTGAGTGG TTATTCTCGG 
GCGCCCTCGA AACGCTCCCA AATCCTCACC CCGTATCGCA CCCAACAGGC AGCCCTGCTC 
CACAAAACCG GAGCGGGTTC CGCGTCCATG CAGAATATGA TGCCATTGGC GTATCTGCCT 
CCGCGGAGGA GTTCCGCCGC GGGATTAGGT CATGGATCTG GTTCCGGCAT GGGCAGTGGC 
TATCGATCGC ACAGTGTGGA TGGGCTTCTA GATCAGGCGG GCTCAACTCC AGAGCAAAGA 
ATAGCAGCAG CTGCGGCCAA GGTGACAGCG GGTAGTACAG CTCTAACGAA CGCCAGTTCC 
ACAAGCACTC TGCAGCCCGA AGAAGAGGTG ACGGATGCCG CCCTCAATGA CTCGGTGACC 
CGGCGTGACA AGCATACTTT ATCCCCACCG GGAAATGTAC AGCAGCACAG AAAGTCCCGA 
AGTCTGGATC ATATTCTCAG CAAACAGACT TTAGCAGAGC TGCTTCCACC GAGTAGCGAA 
CTCGCAGATG GAACGCAAAG TATGCAAAAC CTGGCCATTC CAATGACCCC GCAGCCCCAA 
CGAAGGGACA CCAGCTCCTC CTCGAAATCC CCCACTCCCG AAAGACCTCC GCAACCAGCG 
ATGGAAAGGG TAAGGGAACG CCAGAGTCCT GAGGGTGTGA GTGCGACGGA AAGCGAGCGG 
GAGGATCAGC CAGAAGAATG TCTGCGACAT GGCAACCAAC AGAGGGCATC TGCCTCGGTT 
CATCGGGGAG CCAGTTTGAC CAGCAACAAG ACCTCCAACT CCTCACTGGG CTCCAACTTT 
AGTGCCGGAG GAAACAACAA GACGATATTT AATCGAACGA TGAAGAAGGT CCGCTCGCTG 
ATAAAAAAAC CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Ect4 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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