exex cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	exex
Entrez Gene ID	38884
Full Name	extra-extra
Synonyms	CG8254,DHB9,Dmel\CG8254,HB9,Hb9,dHb9,dhb9,hb9
General protein information	Preferred Names extra-extra Names CG8254 gene product from transcript CG8254-RA CG8254-PA exex-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 66A21-66A21

mRNA and Protein(s)

mRNA	Protein	Name
NM_139907.3	NP_648164.1	extra-extra

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

found that segment specific cell death of some dHb9 expressing motor neurons occurs throughout the metamorphosis period and continues into the post-eclosion period
Title: dHb9 expressing larval motor neurons persist through metamorphosis to innervate adult-specific muscle targets and function in Drosophila eclosion.

Data identify Robo2 and Robo3 as key effectors of Hb9 in regulating nervous system development.
Title: The homeodomain transcription factor Hb9 controls axon guidance in Drosophila through the regulation of Robo receptors.

Hb9 governs neuronal specification and differentiation and activates expression of Nos and fd59a in the Drosophila CNS
Title: Genome-wide identification of Drosophila Hb9 targets reveals a pivotal role in directing the transcriptome within eight neuronal lineages, including activation of nitric oxide synthase and Fd59a/Fox-D.

the single ortholog of Dbx1/2 contributes to the development of specific subsets of interneurons via cross-repressive, lineage-specific interactions with the motoneuron-promoting factors eve and hb9 (exex)
Title: dbx mediates neuronal specification and differentiation through cross-repressive, lineage-specific interactions with eve and hb9.

Drosophila HB9 is required in a subset of motoneurons and interneurons for establishing proper axon projections
Title: Drosophila HB9 is expressed in a subset of motoneurons and interneurons, where it regulates gene expression and axon pathfinding.

The following exex gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the exex cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa17848	NM_139907.3 Latest version!	Drosophila melanogaster extra-extra (exex), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

exex ( NM_139907.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648164.1 Drosophila melanogaster extra-extra (exex), mRNA.

Price & Availability↑

CloneID	OFa17848
Clone ID Related Accession (Same CDS sequence)	NM_139907.3
Accession Version	NM_139907.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	extra-extra
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_139907.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAGTG CCCAAGTGTC GCAACAGCCG CCTCCACCGC CGCTGACCGC CAGCAGTAGC 
ACCTCAATGC CGTCGCAATT GCTACTTAGC CACATTCAGC ACCACAGCAG CAGTCTGCAC 
CATCAGCGCA GTCATCAGCG ATCATCGCCA CCTGCCCAGG ACTACGATCA GGGCAGCAAT 
CAATCAACAC TCTCGCCCAG ATCCCAGATA ACACCCAGTC CGCCGGCAGC CAGTCCCACG 
CATTCGGCGG CCACCACAGG ATCACCAGTT AGCACCTATA CACCGGCCTT GAGTCCCGGT 
GGTGCACAGG ATAGCAGCCA AACGGAGAGT GGTCAGAGTT CACAGATGCC TCTACTAGTG 
GCTCCCATGC CCGTGGGCAC TGGTCCGCCA CATTCACATC CACCGCCGCC GCATCCTCGT 
CTCTATGTGG AGGGTTTCCC GCCACCGCAT CATGTGCCAC ATCCACATCC CGCCCTGGGC 
ACCTATCCTC TGCCACGACT GCCCCTTATG TTACCGGCAA ATCCACCAGG TGTGCCATCG 
GGAGCGGCTG CACCACCGCC CGTCTCACCG CAACCCGCTG CCAATCACCT GAGCCACAGT 
GGAGCCACCA TGGCCACAAC CACACTCCTG CCACCCGCCC AAAGTCAGCC CAAGAAATCC 
TTCTGCATCG ATGCTTTGCT GGCCAAGAGC CAACACCAGT CGGGTGAGCC ACAGCCCATC 
ATTGTGGATG ATCGTCTGGC TGCCCTGCAC TATGCCCGGG ATCAGGCGGA ACTCAATCAT 
GCCTTTGTGA CCGCCTCCAA TGCGGCGGCA GCTGCTCAGG CGGCTGCCTC GGCCGCCGGC 
GGCATCAGCG AACAGGAGGC ACTGCAGCGC ATCCGGGACT CCAGGGAGTA CGACTCACCC 
AGTCCCGACG GCATGTCGAG ATCAGAGTCG CCCACTTCGT CGCACCGCTC CAGTCCGCCC 
ATCAGTCCCG GTTGTGAGGA TCAGCAGCAG CCCCACGGTG CACTGCATCC CCAGCATCTG 
TCCATGCGCA TGTCCGACTT CCACGACGAG TTCAAGAAGC CAATACCACC CCACAGTCCC 
ATCAGACCCC AGGATTTTCC GCTCTACGCA GGAGGACATC CCTACCAGCT GCTGGCCCAA 
GGTGGATCCG CCTTCCACAG ACCTCTGGAT CCCTCAGGGA AACCCATTCC GATACCCATG 
GGTCATAATT TCATGCCGTC GCAGCTGCAA TTCGAATTCC TGGCCCGCGC CGGAATGCTG 
CACCATCGAA TTCCCGAACT GGCCGCCTAT CCGCATCATG CGATTCTGGG CAAGACCAGG 
AGACCACGAA CGGCCTTTAC ATCGCAGCAA CTCCTCGAGC TGGAGAAGCA ATTCAAGCAG 
AACAAGTATC TGAGTCGGCC CAAACGATTC GAGGTGGCCA GCGGTCTGAT GCTGTCCGAG 
ACGCAGGTGA AGATCTGGTT CCAGAATCGT CGGATGAAGT GGAAGCGCTC CAAGAAGGCG 
CAGCAGGAGG CCAAGGAGCG GGCAAAGGCC AACCAGCAGC AGCAGCAGCA GCAACAGACG 
CCCAGTGCCG CCAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648164.1
CDS	181..1758
Translation	MSSAQVSQQPPPPPLTASSSTSMPSQLLLSHIQHHSSSLHHQRSHQRSSPPAQDYDQGSNQSTLSPRSQITPSPPAASPTHSAATTGSPVSTYTPALSPGGAQDSSQTESGQSSQMPLLVAPMPVGTGPPHSHPPPPHPRLYVEGFPPPHHVPHPHPALGTYPLPRLPLMLPANPPGVPSGAAAPPPVSPQPAANHLSHSGATMATTTLLPPAQSQPKKSFCIDALLAKSQHQSGEPQPIIVDDRLAALHYARDQAELNHAFVTASNAAAAAQAAASAAGGISEQEALQRIRDSREYDSPSPDGMSRSESPTSSHRSSPPISPGCEDQQQPHGALHPQHLSMRMSDFHDEFKKPIPPHSPIRPQDFPLYAGGHPYQLLAQGGSAFHRPLDPSGKPIPIPMGHNFMPSQLQFEFLARAGMLHHRIPELAAYPHHAILGKTRRPRTAFTSQQLLELEKQFKQNKYLSRPKRFEVASGLMLSETQVKIWFQNRRMKWKRSKKAQQEAKERAKANQQQQQQQQTPSAAS

Target ORF information:

RefSeq Version	NM_139907.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster extra-extra (exex), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139907.3

ORF Insert Sequence:

ATGTCCAGTG CCCAAGTGTC GCAACAGCCG CCTCCACCGC CGCTGACCGC CAGCAGTAGC 
ACCTCAATGC CGTCGCAATT GCTACTTAGC CACATTCAGC ACCACAGCAG CAGTCTGCAC 
CATCAGCGCA GTCATCAGCG ATCATCGCCA CCTGCCCAGG ACTACGATCA GGGCAGCAAT 
CAATCAACAC TCTCGCCCAG ATCCCAGATA ACACCCAGTC CGCCGGCAGC CAGTCCCACG 
CATTCGGCGG CCACCACAGG ATCACCAGTT AGCACCTATA CACCGGCCTT GAGTCCCGGT 
GGTGCACAGG ATAGCAGCCA AACGGAGAGT GGTCAGAGTT CACAGATGCC TCTACTAGTG 
GCTCCCATGC CCGTGGGCAC TGGTCCGCCA CATTCACATC CACCGCCGCC GCATCCTCGT 
CTCTATGTGG AGGGTTTCCC GCCACCGCAT CATGTGCCAC ATCCACATCC CGCCCTGGGC 
ACCTATCCTC TGCCACGACT GCCCCTTATG TTACCGGCAA ATCCACCAGG TGTGCCATCG 
GGAGCGGCTG CACCACCGCC CGTCTCACCG CAACCCGCTG CCAATCACCT GAGCCACAGT 
GGAGCCACCA TGGCCACAAC CACACTCCTG CCACCCGCCC AAAGTCAGCC CAAGAAATCC 
TTCTGCATCG ATGCTTTGCT GGCCAAGAGC CAACACCAGT CGGGTGAGCC ACAGCCCATC 
ATTGTGGATG ATCGTCTGGC TGCCCTGCAC TATGCCCGGG ATCAGGCGGA ACTCAATCAT 
GCCTTTGTGA CCGCCTCCAA TGCGGCGGCA GCTGCTCAGG CGGCTGCCTC GGCCGCCGGC 
GGCATCAGCG AACAGGAGGC ACTGCAGCGC ATCCGGGACT CCAGGGAGTA CGACTCACCC 
AGTCCCGACG GCATGTCGAG ATCAGAGTCG CCCACTTCGT CGCACCGCTC CAGTCCGCCC 
ATCAGTCCCG GTTGTGAGGA TCAGCAGCAG CCCCACGGTG CACTGCATCC CCAGCATCTG 
TCCATGCGCA TGTCCGACTT CCACGACGAG TTCAAGAAGC CAATACCACC CCACAGTCCC 
ATCAGACCCC AGGATTTTCC GCTCTACGCA GGAGGACATC CCTACCAGCT GCTGGCCCAA 
GGTGGATCCG CCTTCCACAG ACCTCTGGAT CCCTCAGGGA AACCCATTCC GATACCCATG 
GGTCATAATT TCATGCCGTC GCAGCTGCAA TTCGAATTCC TGGCCCGCGC CGGAATGCTG 
CACCATCGAA TTCCCGAACT GGCCGCCTAT CCGCATCATG CGATTCTGGG CAAGACCAGG 
AGACCACGAA CGGCCTTTAC ATCGCAGCAA CTCCTCGAGC TGGAGAAGCA ATTCAAGCAG 
AACAAGTATC TGAGTCGGCC CAAACGATTC GAGGTGGCCA GCGGTCTGAT GCTGTCCGAG 
ACGCAGGTGA AGATCTGGTT CCAGAATCGT CGGATGAAGT GGAAGCGCTC CAAGAAGGCG 
CAGCAGGAGG CCAAGGAGCG GGCAAAGGCC AACCAGCAGC AGCAGCAGCA GCAACAGACG 
CCCAGTGCCG CCAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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exex cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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