smid cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	smid
Entrez Gene ID	38824
Full Name	smallminded
Synonyms	CG8571,Dmel\CG8571,Smid,l(3)j6B8,l(3)j8A6,l(3)s2898
General protein information	Preferred Names smallminded Names CG8571 gene product from transcript CG8571-RI CG8571-PA CG8571-PC CG8571-PG CG8571-PH CG8571-PI small-minded smid-PA smid-PC smid-PG smid-PH smid-PI
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 65F9-65F10

mRNA and Protein(s)

mRNA	Protein	Name
NM_079235.4	NP_523959.2	smallminded, isoform A
NM_001274584.1	NP_001261513.1	smallminded, isoform H
NM_206287.3	NP_996009.2	smallminded, isoform I
NM_001169900.2	NP_001163371.1	smallminded, isoform C
NM_001274583.1	NP_001261512.1	smallminded, isoform G

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Nvl	NP_080447.1
Gallus gallus (chicken)	NVL	NP_001073044.2
Caenorhabditis elegans (roundworm)	mac-1	NP_496814.1
	NVL	XP_001096627.1
Danio rerio (zebrafish)	nvl	NP_998649.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006205	XP_316268.4
Arabidopsis thaliana (thale cress)	CDC48C	NP_186810.2
Xenopus tropicalis (tropical clawed frog)	nvl	NP_001096509.1
Homo sapiens (human)	NVL	NP_002524.2
Bos taurus (cattle)	NVL	XP_002694038.1
Saccharomyces cerevisiae (baker's yeast)	RIX7	NP_013066.1
Kluyveromyces lactis	KLLA0E00837g	XP_453984.1
Schizosaccharomyces pombe (fission yeast)	SPBC16E9.10c	NP_595792.1
Oryza sativa (rice)	Os06g0109400	NP_001056576.1
Pan troglodytes (chimpanzee)	NVL	XP_514229.3
Canis lupus familiaris (dog)	NVL	XP_005622704.1
Drosophila melanogaster (fruit fly)	smid	NP_001163371.1
Eremothecium gossypii	AGOS_AFR188W	NP_985735.2
Rattus norvegicus (Norway rat)	Nvl	NP_001099450.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following smid gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the smid cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa17795	NM_206287.3 Latest version!	Drosophila melanogaster smallminded (smid), transcript variant I, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa17795	NM_001274583.1 Latest version!	Drosophila melanogaster smallminded (smid), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa17796	NM_001169900.2 Latest version!	Drosophila melanogaster smallminded (smid), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa17797	NM_001274584.1 Latest version!	Drosophila melanogaster smallminded (smid), transcript variant H, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa20646	NM_079235.4 Latest version!	Drosophila melanogaster smallminded (smid), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

smid ( NM_206287.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996009.2 Drosophila melanogaster smallminded (smid), transcript variant I, mRNA.

Price & Availability↑

CloneID	OFa17795
Clone ID Related Accession (Same CDS sequence)	NM_206287.3 , NM_001274583.1
Accession Version	NM_206287.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	smallminded, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_206287.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCAGGG AGTTGATGCA AAAGTATCCG GAATATTCAC GACGCAAATT CGGACCATTC 
AGACAGCTGG TGCACCAAGC ATTCTCGATC ATTTCGGAAA GCTATAACTT GGACAAGGTA 
AGCAGTTCCG AGGAAGATTG TGTTAGCGAG GACTCCGAGC CACCACCCAC GAATAGTGTG 
ATGAACAATA TGATGAACAG CCTGTATAGT CAACCAAGAA AGCCGCTGGC GCCCAAGCCA 
ATCAGCGAGC CCATCGATAT ATCCAGTGGA GACGAGAACG AAGATGACTC CAATACAAAA 
ACCACCAACG GAGATGGGGT AGCTGCTGCT GCTGCTCCTC CTCCTCCTAC TCCAGCAGTG 
CAGGGCAGTG CTTTGAAACG CCTGATGGAG GAAGTGCCGG AGATCGCAGT GGCTGCAAAG 
AAAGTAGCTA AACCGAATAC TATTCACGTT AGCAGTTCGG AGGCCATTCA AAAGCTGCAC 
CAAGTTGTGG GCAACAGAGC GAAAAATCTT TCAGAGGACG CCGTTCCGCG TTCCAAAGAT 
CACCGTAATG TGCCTGGCTT ATATCAGCAG TTGCACCAAA ACCAAAGTCG CGATCGCCTT 
CGGAAGTTCA AAAGGGATCT GGAGGTGCAG CACCCAACGG AAAGTTTCCG GGACATTGGT 
GGCATGGATA GCACTCTTAA GGAGTTGTGT GAAATGCTGA TCCATATTAA GTCGCCGGAG 
TTCTACTTTC AATTGGGTCT ATTGCCTTCG AGAGGTTTAT TGCTTCATGG GCCGCCAGGC 
TGTGGCAAAA CCTTTCTTGC TCGTGCCATT AGCGGGCAAC TAAAAATGCC TCTAATGGAA 
ATTCCGGCCA CAGAGCTAAT TGGTGGCATC TCTGGTGAGT CGGAAGAACG GATTCGCGAG 
GTCTTTGACC AGGCCATTGG CTACTCGCCT TGTGTGCTTT TCATCGATGA AATCGACGCC 
ATCGGAGGAA ATCGTCAGTG GGCGTCCAAG GATATGGAAC GTCGCATTGT CTCGCAGCTG 
ATAAGCAGTC TGGACAACCT GAAGGCCAAC GAGTTTGGCC AATCAGTAGT CGTCATAGCG 
GCCACCACGC GTCCCGATGT CCTCGACCCT GGACTACGTC GCATCGGTCG CTTCGATCAC 
GAAATTGCCA TACACATCCC ATCGCGGAAA GAGCGGCGTG AAATCTTGCG CATCCAGTGC 
GAGGGCTTGT CCGTCGATCC CAAGCTAAAT TATGATAAGA TTGCAGAGCT AACACCGGGA 
TATGTCGGTG CTGATCTAAT GGCTCTCGTA AGTCGTGCAG CTTCTGTGGC CGTAAAGCGT 
AGGTCCATGA AGAAGTTCCG TGAACTGCAC GCTGCTAGTG AAAAGAATAT GACAACGGTC 
ACTTTGGATG ACGACGAGCC CAGCGAGGAC GCAGGTGAAA CACCTGTGCC TGATTCAAAA 
GGTGAAGAAA CTGCAAAAGA TGCAGAGGCA GAACAAAAGG TAGATGGTGA CAAGGAAACA 
TCTGCTAAGG ATAAATCAGA AGGCGACTCG CCAAATATTG AAACCCCCAA GAAAGCGACC 
AACGGCAACT CATCGATAAA GTCGCCGCAG AAAACACCGA AGAAATCAGC GGAGAAACCA 
ACTGACGCAG CGATGGATGT AGACAATGTT GCTCCCGAGG AGCCGAAAAA AGCCGTTGAG 
CAGGAGGTGG ATTCCTCCTC CTCCAACGAC GAGTACTACG AACCCACACT GGCCGAACTG 
ACCAATTTTC TTGACAACCC CCCTGAAGAG TTCGCCGACC CAAACTTTTG CCTCACTCTC 
ATTGATTTCG TAGATGCCAT TAAGGTGATG CAGCCTTCGG CCAAGCGCGA GGGCTTTATA 
ACAGTACCAG ATACCACTTG GGATGACATT GGTGCCCTAG AGAAAATTCG CGAAGAGCTT 
AAGTTGGCTG TCTTGGCACC AGTTAAGTAT CCAGAGATGC TGGAACGTCT TGGGCTGACA 
GCTCCGTCAG GCGTTCTGCT ATGCGGTCCG CCGGGTTGCG GCAAAACTCT TTTGGCCAAG 
GCTATTGCCA ATGAGGCTGG CATCAATTTC ATATCGGTGA AGGGCCCCGA ACTTATGAAC 
ATGTATGTCG GCGAGAGCGA ACGAGCTGTG CGTGCCTGTT TCCAACGTGC CCGGAACTCT 
GCACCCTGCG TCATCTTCTT CGACGAGTTC GACTCCTTGT GCCCCAAGCG ATCGGATGGT 
GGCGATGGTA ATAATTCGGG CACTCGTATC GTTAACCAAC TGCTGACCGA AATGGATGGC 
GTCGAGGAGC GCAAGGGTGT ATACATATTG GCGGCCACCA ACAGACCGGA TATCATTGAT 
CCGGCCATCC TGCGACCGGG TCGCCTGGAC ACCATTTTGT ACGTGGGTTT CCCCGAGCAG 
AGCGAACGTA CCGAGATTTT GAAGGCCACC ACAAAGAACG GAAAGCGTCC GGTTCTGGCC 
GATGATGTAG ATCTCGATGA AATTGCTGCC CAAACCGAGG GCTATACTGG CGCAGATTTG 
GCAGGATTGG TCAAGCAGGC CTCCATGTTC TCCCTGCGAC AATCTCTAAA CAACGGCGAC 
ACAAATCTCG ACGATTTGTG CGTACGCAGC CAGCATTTCC AGGAAGCTTT GCAGCAACTT 
CGGCCCTCAG TTAACGAACA GGACCGCAAA ATATACGATA AACTGCGCCT GAAATATGCT 
GCACCAAGGG TACCCACTCT AAATGATAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996009.2
CDS	119..2851
Translation	MTRELMQKYPEYSRRKFGPFRQLVHQAFSIISESYNLDKVSSSEEDCVSEDSEPPPTNSVMNNMMNSLYSQPRKPLAPKPISEPIDISSGDENEDDSNTKTTNGDGVAAAAAPPPPTPAVQGSALKRLMEEVPEIAVAAKKVAKPNTIHVSSSEAIQKLHQVVGNRAKNLSEDAVPRSKDHRNVPGLYQQLHQNQSRDRLRKFKRDLEVQHPTESFRDIGGMDSTLKELCEMLIHIKSPEFYFQLGLLPSRGLLLHGPPGCGKTFLARAISGQLKMPLMEIPATELIGGISGESEERIREVFDQAIGYSPCVLFIDEIDAIGGNRQWASKDMERRIVSQLISSLDNLKANEFGQSVVVIAATTRPDVLDPGLRRIGRFDHEIAIHIPSRKERREILRIQCEGLSVDPKLNYDKIAELTPGYVGADLMALVSRAASVAVKRRSMKKFRELHAASEKNMTTVTLDDDEPSEDAGETPVPDSKGEETAKDAEAEQKVDGDKETSAKDKSEGDSPNIETPKKATNGNSSIKSPQKTPKKSAEKPTDAAMDVDNVAPEEPKKAVEQEVDSSSSNDEYYEPTLAELTNFLDNPPEEFADPNFCLTLIDFVDAIKVMQPSAKREGFITVPDTTWDDIGALEKIREELKLAVLAPVKYPEMLERLGLTAPSGVLLCGPPGCGKTLLAKAIANEAGINFISVKGPELMNMYVGESERAVRACFQRARNSAPCVIFFDEFDSLCPKRSDGGDGNNSGTRIVNQLLTEMDGVEERKGVYILAATNRPDIIDPAILRPGRLDTILYVGFPEQSERTEILKATTKNGKRPVLADDVDLDEIAAQTEGYTGADLAGLVKQASMFSLRQSLNNGDTNLDDLCVRSQHFQEALQQLRPSVNEQDRKIYDKLRLKYAAPRVPTLNDK

Target ORF information:

RefSeq Version	NM_206287.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster smallminded (smid), transcript variant I, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206287.3

ORF Insert Sequence:

ATGACCAGGG AGTTGATGCA AAAGTATCCG GAATATTCAC GACGCAAATT CGGACCATTC 
AGACAGCTGG TGCACCAAGC ATTCTCGATC ATTTCGGAAA GCTATAACTT GGACAAGGTA 
AGCAGTTCCG AGGAAGATTG TGTTAGCGAG GACTCCGAGC CACCACCCAC GAATAGTGTG 
ATGAACAATA TGATGAACAG CCTGTATAGT CAACCAAGAA AGCCGCTGGC GCCCAAGCCA 
ATCAGCGAGC CCATCGATAT ATCCAGTGGA GACGAGAACG AAGATGACTC CAATACAAAA 
ACCACCAACG GAGATGGGGT AGCTGCTGCT GCTGCTCCTC CTCCTCCTAC TCCAGCAGTG 
CAGGGCAGTG CTTTGAAACG CCTGATGGAG GAAGTGCCGG AGATCGCAGT GGCTGCAAAG 
AAAGTAGCTA AACCGAATAC TATTCACGTT AGCAGTTCGG AGGCCATTCA AAAGCTGCAC 
CAAGTTGTGG GCAACAGAGC GAAAAATCTT TCAGAGGACG CCGTTCCGCG TTCCAAAGAT 
CACCGTAATG TGCCTGGCTT ATATCAGCAG TTGCACCAAA ACCAAAGTCG CGATCGCCTT 
CGGAAGTTCA AAAGGGATCT GGAGGTGCAG CACCCAACGG AAAGTTTCCG GGACATTGGT 
GGCATGGATA GCACTCTTAA GGAGTTGTGT GAAATGCTGA TCCATATTAA GTCGCCGGAG 
TTCTACTTTC AATTGGGTCT ATTGCCTTCG AGAGGTTTAT TGCTTCATGG GCCGCCAGGC 
TGTGGCAAAA CCTTTCTTGC TCGTGCCATT AGCGGGCAAC TAAAAATGCC TCTAATGGAA 
ATTCCGGCCA CAGAGCTAAT TGGTGGCATC TCTGGTGAGT CGGAAGAACG GATTCGCGAG 
GTCTTTGACC AGGCCATTGG CTACTCGCCT TGTGTGCTTT TCATCGATGA AATCGACGCC 
ATCGGAGGAA ATCGTCAGTG GGCGTCCAAG GATATGGAAC GTCGCATTGT CTCGCAGCTG 
ATAAGCAGTC TGGACAACCT GAAGGCCAAC GAGTTTGGCC AATCAGTAGT CGTCATAGCG 
GCCACCACGC GTCCCGATGT CCTCGACCCT GGACTACGTC GCATCGGTCG CTTCGATCAC 
GAAATTGCCA TACACATCCC ATCGCGGAAA GAGCGGCGTG AAATCTTGCG CATCCAGTGC 
GAGGGCTTGT CCGTCGATCC CAAGCTAAAT TATGATAAGA TTGCAGAGCT AACACCGGGA 
TATGTCGGTG CTGATCTAAT GGCTCTCGTA AGTCGTGCAG CTTCTGTGGC CGTAAAGCGT 
AGGTCCATGA AGAAGTTCCG TGAACTGCAC GCTGCTAGTG AAAAGAATAT GACAACGGTC 
ACTTTGGATG ACGACGAGCC CAGCGAGGAC GCAGGTGAAA CACCTGTGCC TGATTCAAAA 
GGTGAAGAAA CTGCAAAAGA TGCAGAGGCA GAACAAAAGG TAGATGGTGA CAAGGAAACA 
TCTGCTAAGG ATAAATCAGA AGGCGACTCG CCAAATATTG AAACCCCCAA GAAAGCGACC 
AACGGCAACT CATCGATAAA GTCGCCGCAG AAAACACCGA AGAAATCAGC GGAGAAACCA 
ACTGACGCAG CGATGGATGT AGACAATGTT GCTCCCGAGG AGCCGAAAAA AGCCGTTGAG 
CAGGAGGTGG ATTCCTCCTC CTCCAACGAC GAGTACTACG AACCCACACT GGCCGAACTG 
ACCAATTTTC TTGACAACCC CCCTGAAGAG TTCGCCGACC CAAACTTTTG CCTCACTCTC 
ATTGATTTCG TAGATGCCAT TAAGGTGATG CAGCCTTCGG CCAAGCGCGA GGGCTTTATA 
ACAGTACCAG ATACCACTTG GGATGACATT GGTGCCCTAG AGAAAATTCG CGAAGAGCTT 
AAGTTGGCTG TCTTGGCACC AGTTAAGTAT CCAGAGATGC TGGAACGTCT TGGGCTGACA 
GCTCCGTCAG GCGTTCTGCT ATGCGGTCCG CCGGGTTGCG GCAAAACTCT TTTGGCCAAG 
GCTATTGCCA ATGAGGCTGG CATCAATTTC ATATCGGTGA AGGGCCCCGA ACTTATGAAC 
ATGTATGTCG GCGAGAGCGA ACGAGCTGTG CGTGCCTGTT TCCAACGTGC CCGGAACTCT 
GCACCCTGCG TCATCTTCTT CGACGAGTTC GACTCCTTGT GCCCCAAGCG ATCGGATGGT 
GGCGATGGTA ATAATTCGGG CACTCGTATC GTTAACCAAC TGCTGACCGA AATGGATGGC 
GTCGAGGAGC GCAAGGGTGT ATACATATTG GCGGCCACCA ACAGACCGGA TATCATTGAT 
CCGGCCATCC TGCGACCGGG TCGCCTGGAC ACCATTTTGT ACGTGGGTTT CCCCGAGCAG 
AGCGAACGTA CCGAGATTTT GAAGGCCACC ACAAAGAACG GAAAGCGTCC GGTTCTGGCC 
GATGATGTAG ATCTCGATGA AATTGCTGCC CAAACCGAGG GCTATACTGG CGCAGATTTG 
GCAGGATTGG TCAAGCAGGC CTCCATGTTC TCCCTGCGAC AATCTCTAAA CAACGGCGAC 
ACAAATCTCG ACGATTTGTG CGTACGCAGC CAGCATTTCC AGGAAGCTTT GCAGCAACTT 
CGGCCCTCAG TTAACGAACA GGACCGCAAA ATATACGATA AACTGCGCCT GAAATATGCT 
GCACCAAGGG TACCCACTCT AAATGATAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smid ( NM_001274583.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261512.1 Drosophila melanogaster smallminded (smid), transcript variant G, mRNA.

Price & Availability↑

CloneID	OFa17795
Clone ID Related Accession (Same CDS sequence)	NM_206287.3 , NM_001274583.1
Accession Version	NM_001274583.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	smallminded, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCAGGG AGTTGATGCA AAAGTATCCG GAATATTCAC GACGCAAATT CGGACCATTC 
AGACAGCTGG TGCACCAAGC ATTCTCGATC ATTTCGGAAA GCTATAACTT GGACAAGGTA 
AGCAGTTCCG AGGAAGATTG TGTTAGCGAG GACTCCGAGC CACCACCCAC GAATAGTGTG 
ATGAACAATA TGATGAACAG CCTGTATAGT CAACCAAGAA AGCCGCTGGC GCCCAAGCCA 
ATCAGCGAGC CCATCGATAT ATCCAGTGGA GACGAGAACG AAGATGACTC CAATACAAAA 
ACCACCAACG GAGATGGGGT AGCTGCTGCT GCTGCTCCTC CTCCTCCTAC TCCAGCAGTG 
CAGGGCAGTG CTTTGAAACG CCTGATGGAG GAAGTGCCGG AGATCGCAGT GGCTGCAAAG 
AAAGTAGCTA AACCGAATAC TATTCACGTT AGCAGTTCGG AGGCCATTCA AAAGCTGCAC 
CAAGTTGTGG GCAACAGAGC GAAAAATCTT TCAGAGGACG CCGTTCCGCG TTCCAAAGAT 
CACCGTAATG TGCCTGGCTT ATATCAGCAG TTGCACCAAA ACCAAAGTCG CGATCGCCTT 
CGGAAGTTCA AAAGGGATCT GGAGGTGCAG CACCCAACGG AAAGTTTCCG GGACATTGGT 
GGCATGGATA GCACTCTTAA GGAGTTGTGT GAAATGCTGA TCCATATTAA GTCGCCGGAG 
TTCTACTTTC AATTGGGTCT ATTGCCTTCG AGAGGTTTAT TGCTTCATGG GCCGCCAGGC 
TGTGGCAAAA CCTTTCTTGC TCGTGCCATT AGCGGGCAAC TAAAAATGCC TCTAATGGAA 
ATTCCGGCCA CAGAGCTAAT TGGTGGCATC TCTGGTGAGT CGGAAGAACG GATTCGCGAG 
GTCTTTGACC AGGCCATTGG CTACTCGCCT TGTGTGCTTT TCATCGATGA AATCGACGCC 
ATCGGAGGAA ATCGTCAGTG GGCGTCCAAG GATATGGAAC GTCGCATTGT CTCGCAGCTG 
ATAAGCAGTC TGGACAACCT GAAGGCCAAC GAGTTTGGCC AATCAGTAGT CGTCATAGCG 
GCCACCACGC GTCCCGATGT CCTCGACCCT GGACTACGTC GCATCGGTCG CTTCGATCAC 
GAAATTGCCA TACACATCCC ATCGCGGAAA GAGCGGCGTG AAATCTTGCG CATCCAGTGC 
GAGGGCTTGT CCGTCGATCC CAAGCTAAAT TATGATAAGA TTGCAGAGCT AACACCGGGA 
TATGTCGGTG CTGATCTAAT GGCTCTCGTA AGTCGTGCAG CTTCTGTGGC CGTAAAGCGT 
AGGTCCATGA AGAAGTTCCG TGAACTGCAC GCTGCTAGTG AAAAGAATAT GACAACGGTC 
ACTTTGGATG ACGACGAGCC CAGCGAGGAC GCAGGTGAAA CACCTGTGCC TGATTCAAAA 
GGTGAAGAAA CTGCAAAAGA TGCAGAGGCA GAACAAAAGG TAGATGGTGA CAAGGAAACA 
TCTGCTAAGG ATAAATCAGA AGGCGACTCG CCAAATATTG AAACCCCCAA GAAAGCGACC 
AACGGCAACT CATCGATAAA GTCGCCGCAG AAAACACCGA AGAAATCAGC GGAGAAACCA 
ACTGACGCAG CGATGGATGT AGACAATGTT GCTCCCGAGG AGCCGAAAAA AGCCGTTGAG 
CAGGAGGTGG ATTCCTCCTC CTCCAACGAC GAGTACTACG AACCCACACT GGCCGAACTG 
ACCAATTTTC TTGACAACCC CCCTGAAGAG TTCGCCGACC CAAACTTTTG CCTCACTCTC 
ATTGATTTCG TAGATGCCAT TAAGGTGATG CAGCCTTCGG CCAAGCGCGA GGGCTTTATA 
ACAGTACCAG ATACCACTTG GGATGACATT GGTGCCCTAG AGAAAATTCG CGAAGAGCTT 
AAGTTGGCTG TCTTGGCACC AGTTAAGTAT CCAGAGATGC TGGAACGTCT TGGGCTGACA 
GCTCCGTCAG GCGTTCTGCT ATGCGGTCCG CCGGGTTGCG GCAAAACTCT TTTGGCCAAG 
GCTATTGCCA ATGAGGCTGG CATCAATTTC ATATCGGTGA AGGGCCCCGA ACTTATGAAC 
ATGTATGTCG GCGAGAGCGA ACGAGCTGTG CGTGCCTGTT TCCAACGTGC CCGGAACTCT 
GCACCCTGCG TCATCTTCTT CGACGAGTTC GACTCCTTGT GCCCCAAGCG ATCGGATGGT 
GGCGATGGTA ATAATTCGGG CACTCGTATC GTTAACCAAC TGCTGACCGA AATGGATGGC 
GTCGAGGAGC GCAAGGGTGT ATACATATTG GCGGCCACCA ACAGACCGGA TATCATTGAT 
CCGGCCATCC TGCGACCGGG TCGCCTGGAC ACCATTTTGT ACGTGGGTTT CCCCGAGCAG 
AGCGAACGTA CCGAGATTTT GAAGGCCACC ACAAAGAACG GAAAGCGTCC GGTTCTGGCC 
GATGATGTAG ATCTCGATGA AATTGCTGCC CAAACCGAGG GCTATACTGG CGCAGATTTG 
GCAGGATTGG TCAAGCAGGC CTCCATGTTC TCCCTGCGAC AATCTCTAAA CAACGGCGAC 
ACAAATCTCG ACGATTTGTG CGTACGCAGC CAGCATTTCC AGGAAGCTTT GCAGCAACTT 
CGGCCCTCAG TTAACGAACA GGACCGCAAA ATATACGATA AACTGCGCCT GAAATATGCT 
GCACCAAGGG TACCCACTCT AAATGATAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261512.1
CDS	352..3084
Translation	MTRELMQKYPEYSRRKFGPFRQLVHQAFSIISESYNLDKVSSSEEDCVSEDSEPPPTNSVMNNMMNSLYSQPRKPLAPKPISEPIDISSGDENEDDSNTKTTNGDGVAAAAAPPPPTPAVQGSALKRLMEEVPEIAVAAKKVAKPNTIHVSSSEAIQKLHQVVGNRAKNLSEDAVPRSKDHRNVPGLYQQLHQNQSRDRLRKFKRDLEVQHPTESFRDIGGMDSTLKELCEMLIHIKSPEFYFQLGLLPSRGLLLHGPPGCGKTFLARAISGQLKMPLMEIPATELIGGISGESEERIREVFDQAIGYSPCVLFIDEIDAIGGNRQWASKDMERRIVSQLISSLDNLKANEFGQSVVVIAATTRPDVLDPGLRRIGRFDHEIAIHIPSRKERREILRIQCEGLSVDPKLNYDKIAELTPGYVGADLMALVSRAASVAVKRRSMKKFRELHAASEKNMTTVTLDDDEPSEDAGETPVPDSKGEETAKDAEAEQKVDGDKETSAKDKSEGDSPNIETPKKATNGNSSIKSPQKTPKKSAEKPTDAAMDVDNVAPEEPKKAVEQEVDSSSSNDEYYEPTLAELTNFLDNPPEEFADPNFCLTLIDFVDAIKVMQPSAKREGFITVPDTTWDDIGALEKIREELKLAVLAPVKYPEMLERLGLTAPSGVLLCGPPGCGKTLLAKAIANEAGINFISVKGPELMNMYVGESERAVRACFQRARNSAPCVIFFDEFDSLCPKRSDGGDGNNSGTRIVNQLLTEMDGVEERKGVYILAATNRPDIIDPAILRPGRLDTILYVGFPEQSERTEILKATTKNGKRPVLADDVDLDEIAAQTEGYTGADLAGLVKQASMFSLRQSLNNGDTNLDDLCVRSQHFQEALQQLRPSVNEQDRKIYDKLRLKYAAPRVPTLNDK

Target ORF information:

RefSeq Version	NM_001274583.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster smallminded (smid), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274583.1

ORF Insert Sequence:

ATGACCAGGG AGTTGATGCA AAAGTATCCG GAATATTCAC GACGCAAATT CGGACCATTC 
AGACAGCTGG TGCACCAAGC ATTCTCGATC ATTTCGGAAA GCTATAACTT GGACAAGGTA 
AGCAGTTCCG AGGAAGATTG TGTTAGCGAG GACTCCGAGC CACCACCCAC GAATAGTGTG 
ATGAACAATA TGATGAACAG CCTGTATAGT CAACCAAGAA AGCCGCTGGC GCCCAAGCCA 
ATCAGCGAGC CCATCGATAT ATCCAGTGGA GACGAGAACG AAGATGACTC CAATACAAAA 
ACCACCAACG GAGATGGGGT AGCTGCTGCT GCTGCTCCTC CTCCTCCTAC TCCAGCAGTG 
CAGGGCAGTG CTTTGAAACG CCTGATGGAG GAAGTGCCGG AGATCGCAGT GGCTGCAAAG 
AAAGTAGCTA AACCGAATAC TATTCACGTT AGCAGTTCGG AGGCCATTCA AAAGCTGCAC 
CAAGTTGTGG GCAACAGAGC GAAAAATCTT TCAGAGGACG CCGTTCCGCG TTCCAAAGAT 
CACCGTAATG TGCCTGGCTT ATATCAGCAG TTGCACCAAA ACCAAAGTCG CGATCGCCTT 
CGGAAGTTCA AAAGGGATCT GGAGGTGCAG CACCCAACGG AAAGTTTCCG GGACATTGGT 
GGCATGGATA GCACTCTTAA GGAGTTGTGT GAAATGCTGA TCCATATTAA GTCGCCGGAG 
TTCTACTTTC AATTGGGTCT ATTGCCTTCG AGAGGTTTAT TGCTTCATGG GCCGCCAGGC 
TGTGGCAAAA CCTTTCTTGC TCGTGCCATT AGCGGGCAAC TAAAAATGCC TCTAATGGAA 
ATTCCGGCCA CAGAGCTAAT TGGTGGCATC TCTGGTGAGT CGGAAGAACG GATTCGCGAG 
GTCTTTGACC AGGCCATTGG CTACTCGCCT TGTGTGCTTT TCATCGATGA AATCGACGCC 
ATCGGAGGAA ATCGTCAGTG GGCGTCCAAG GATATGGAAC GTCGCATTGT CTCGCAGCTG 
ATAAGCAGTC TGGACAACCT GAAGGCCAAC GAGTTTGGCC AATCAGTAGT CGTCATAGCG 
GCCACCACGC GTCCCGATGT CCTCGACCCT GGACTACGTC GCATCGGTCG CTTCGATCAC 
GAAATTGCCA TACACATCCC ATCGCGGAAA GAGCGGCGTG AAATCTTGCG CATCCAGTGC 
GAGGGCTTGT CCGTCGATCC CAAGCTAAAT TATGATAAGA TTGCAGAGCT AACACCGGGA 
TATGTCGGTG CTGATCTAAT GGCTCTCGTA AGTCGTGCAG CTTCTGTGGC CGTAAAGCGT 
AGGTCCATGA AGAAGTTCCG TGAACTGCAC GCTGCTAGTG AAAAGAATAT GACAACGGTC 
ACTTTGGATG ACGACGAGCC CAGCGAGGAC GCAGGTGAAA CACCTGTGCC TGATTCAAAA 
GGTGAAGAAA CTGCAAAAGA TGCAGAGGCA GAACAAAAGG TAGATGGTGA CAAGGAAACA 
TCTGCTAAGG ATAAATCAGA AGGCGACTCG CCAAATATTG AAACCCCCAA GAAAGCGACC 
AACGGCAACT CATCGATAAA GTCGCCGCAG AAAACACCGA AGAAATCAGC GGAGAAACCA 
ACTGACGCAG CGATGGATGT AGACAATGTT GCTCCCGAGG AGCCGAAAAA AGCCGTTGAG 
CAGGAGGTGG ATTCCTCCTC CTCCAACGAC GAGTACTACG AACCCACACT GGCCGAACTG 
ACCAATTTTC TTGACAACCC CCCTGAAGAG TTCGCCGACC CAAACTTTTG CCTCACTCTC 
ATTGATTTCG TAGATGCCAT TAAGGTGATG CAGCCTTCGG CCAAGCGCGA GGGCTTTATA 
ACAGTACCAG ATACCACTTG GGATGACATT GGTGCCCTAG AGAAAATTCG CGAAGAGCTT 
AAGTTGGCTG TCTTGGCACC AGTTAAGTAT CCAGAGATGC TGGAACGTCT TGGGCTGACA 
GCTCCGTCAG GCGTTCTGCT ATGCGGTCCG CCGGGTTGCG GCAAAACTCT TTTGGCCAAG 
GCTATTGCCA ATGAGGCTGG CATCAATTTC ATATCGGTGA AGGGCCCCGA ACTTATGAAC 
ATGTATGTCG GCGAGAGCGA ACGAGCTGTG CGTGCCTGTT TCCAACGTGC CCGGAACTCT 
GCACCCTGCG TCATCTTCTT CGACGAGTTC GACTCCTTGT GCCCCAAGCG ATCGGATGGT 
GGCGATGGTA ATAATTCGGG CACTCGTATC GTTAACCAAC TGCTGACCGA AATGGATGGC 
GTCGAGGAGC GCAAGGGTGT ATACATATTG GCGGCCACCA ACAGACCGGA TATCATTGAT 
CCGGCCATCC TGCGACCGGG TCGCCTGGAC ACCATTTTGT ACGTGGGTTT CCCCGAGCAG 
AGCGAACGTA CCGAGATTTT GAAGGCCACC ACAAAGAACG GAAAGCGTCC GGTTCTGGCC 
GATGATGTAG ATCTCGATGA AATTGCTGCC CAAACCGAGG GCTATACTGG CGCAGATTTG 
GCAGGATTGG TCAAGCAGGC CTCCATGTTC TCCCTGCGAC AATCTCTAAA CAACGGCGAC 
ACAAATCTCG ACGATTTGTG CGTACGCAGC CAGCATTTCC AGGAAGCTTT GCAGCAACTT 
CGGCCCTCAG TTAACGAACA GGACCGCAAA ATATACGATA AACTGCGCCT GAAATATGCT 
GCACCAAGGG TACCCACTCT AAATGATAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smid ( NM_001169900.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163371.1 Drosophila melanogaster smallminded (smid), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa17796
Clone ID Related Accession (Same CDS sequence)	NM_001169900.2
Accession Version	NM_001169900.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	smallminded, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001169900.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGAAAG CCAAGCCGCT GCTGCACGAC CATCTAATAA CGATCCGAGT AAAGAAGTAT 
CTGGAGGAGC ACATCGGGGA AACCTACCTG GATGTGAAAC AGATGACCAG GGAGTTGATG 
CAAAAGTATC CGGAATATTC ACGACGCAAA TTCGGACCAT TCAGACAGCT GGTGCACCAA 
GCATTCTCGA TCATTTCGGA AAGCTATAAC TTGGACAAGG TAAGCAGTTC CGAGGAAGAT 
TGTGTTAGCG AGGACTCCGA GCCACCACCC ACGAATAGTG TGATGAACAA TATGATGAAC 
AGCCTGTATA GTCAACCAAG AAAGCCGCTG GCGCCCAAGC CAATCAGCGA GCCCATCGAT 
ATATCCAGTG GAGACGAGAA CGAAGATGAC TCCAATACAA AAACCACCAA CGGAGATGGG 
GTAGCTGCTG CTGCTGCTCC TCCTCCTCCT ACTCCAGCAG TGCAGGGCAG TGCTTTGAAA 
CGCCTGATGG AGGAAGTGCC GGAGATCGCA GTGGCTGCAA AGAAAGCTAA ACCGAATACT 
ATTCACGTTA GCAGTTCGGA GGCCATTCAA AAGCTGCACC AAGTTGTGGG CAACAGAGCG 
AAAAATCTTT CAGAGGACGC CGTTCCGCGT TCCAAAGATC ACCGTAATGT GCCTGGCTTA 
TATCAGCAGT TGCACCAAAA CCAAAGTCGC GATCGCCTTC GGAAGTTCAA AAGGGATCTG 
GAGGTGCAGC ACCCAACGGA AAGTTTCCGG GACATTGGTG GCATGGATAG CACTCTTAAG 
GAGTTGTGTG AAATGCTGAT CCATATTAAG TCGCCGGAGT TCTACTTTCA ATTGGGTCTA 
TTGCCTTCGA GAGGTTTATT GCTTCATGGG CCGCCAGGCT GTGGCAAAAC CTTTCTTGCT 
CGTGCCATTA GCGGGCAACT AAAAATGCCT CTAATGGAAA TTCCGGCCAC AGAGCTAATT 
GGTGGCATCT CTGGTGAGTC GGAAGAACGG ATTCGCGAGG TCTTTGACCA GGCCATTGGC 
TACTCGCCTT GTGTGCTTTT CATCGATGAA ATCGACGCCA TCGGAGGAAA TCGTCAGTGG 
GCGTCCAAGG ATATGGAACG TCGCATTGTC TCGCAGCTGA TAAGCAGTCT GGACAACCTG 
AAGGCCAACG AGTTTGGCCA ATCAGTAGTC GTCATAGCGG CCACCACGCG TCCCGATGTC 
CTCGACCCTG GACTACGTCG CATCGGTCGC TTCGATCACG AAATTGCCAT ACACATCCCA 
TCGCGGAAAG AGCGGCGTGA AATCTTGCGC ATCCAGTGCG AGGGCTTGTC CGTCGATCCC 
AAGCTAAATT ATGATAAGAT TGCAGAGCTA ACACCGGGAT ATGTCGGTGC TGATCTAATG 
GCTCTCGTAA GTCGTGCAGC TTCTGTGGCC GTAAAGCGTA GGTCCATGAA GAAGTTCCGT 
GAACTGCACG CTGCTAGTGA AAAGAATATG ACAACGGTCA CTTTGGATGA CGACGAGCCC 
AGCGAGGACG CAGGTGAAAC ACCTGTGCCT GATTCAAAAG GTGAAGAAAC TGCAAAAGAT 
GCAGAGGCAG AACAAAAGGT AGATGGTGAC AAGGAAACAT CTGCTAAGGA TAAATCAGAA 
GGCGACTCGC CAAATATTGA AACCCCCAAG AAAGCGACCA ACGGCAACTC ATCGATAAAG 
TCGCCGCAGA AAACACCGAA GAAATCAGCG GAGAAACCAA CTGACGCAGC GATGGATGTA 
GACAATGTTG CTCCCGAGGA GCCGAAAAAA GCCGTTGAGC AGGAGGTGGA TTCCTCCTCC 
TCCAACGACG AGTACTACGA ACCCACACTG GCCGAACTGA CCAATTTTCT TGACAACCCC 
CCTGAAGAGT TCGCCGACCC AAACTTTTGC CTCACTCTCA TTGATTTCGT AGATGCCATT 
AAGGTGATGC AGCCTTCGGC CAAGCGCGAG GGCTTTATAA CAGTACCAGA TACCACTTGG 
GATGACATTG GTGCCCTAGA GAAAATTCGC GAAGAGCTTA AGTTGGCTGT CTTGGCACCA 
GTTAAGTATC CAGAGATGCT GGAACGTCTT GGGCTGACAG CTCCGTCAGG CGTTCTGCTA 
TGCGGTCCGC CGGGTTGCGG CAAAACTCTT TTGGCCAAGG CTATTGCCAA TGAGGCTGGC 
ATCAATTTCA TATCGGTGAA GGGCCCCGAA CTTATGAACA TGTATGTCGG CGAGAGCGAA 
CGAGCTGTGC GTGCCTGTTT CCAACGTGCC CGGAACTCTG CACCCTGCGT CATCTTCTTC 
GACGAGTTCG ACTCCTTGTG CCCCAAGCGA TCGGATGGTG GCGATGGTAA TAATTCGGGC 
ACTCGTATCG TTAACCAACT GCTGACCGAA ATGGATGGCG TCGAGGAGCG CAAGGGTGTA 
TACATATTGG CGGCCACCAA CAGACCGGAT ATCATTGATC CGGCCATCCT GCGACCGGGT 
CGCCTGGACA CCATTTTGTA CGTGGGTTTC CCCGAGCAGA GCGAACGTAC CGAGATTTTG 
AAGGCCACCA CAAAGAACGG AAAGCGTCCG GTTCTGGCCG ATGATGTAGA TCTCGATGAA 
ATTGCTGCCC AAACCGAGGG CTATACTGGC GCAGATTTGG CAGGATTGGT CAAGCAGGCC 
TCCATGTTCT CCCTGCGACA ATCTCTAAAC AACGGCGACA CAAATCTCGA CGATTTGTGC 
GTACGCAGCC AGCATTTCCA GGAAGCTTTG CAGCAACTTC GGCCCTCAGT TAACGAACAG 
GACCGCAAAA TATACGATAA ACTGCGCCTG AAATATGCTG CACCAAGGGT ACCCACTCTA 
AATGATAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163371.1
CDS	107..2938
Translation	MKKAKPLLHDHLITIRVKKYLEEHIGETYLDVKQMTRELMQKYPEYSRRKFGPFRQLVHQAFSIISESYNLDKVSSSEEDCVSEDSEPPPTNSVMNNMMNSLYSQPRKPLAPKPISEPIDISSGDENEDDSNTKTTNGDGVAAAAAPPPPTPAVQGSALKRLMEEVPEIAVAAKKAKPNTIHVSSSEAIQKLHQVVGNRAKNLSEDAVPRSKDHRNVPGLYQQLHQNQSRDRLRKFKRDLEVQHPTESFRDIGGMDSTLKELCEMLIHIKSPEFYFQLGLLPSRGLLLHGPPGCGKTFLARAISGQLKMPLMEIPATELIGGISGESEERIREVFDQAIGYSPCVLFIDEIDAIGGNRQWASKDMERRIVSQLISSLDNLKANEFGQSVVVIAATTRPDVLDPGLRRIGRFDHEIAIHIPSRKERREILRIQCEGLSVDPKLNYDKIAELTPGYVGADLMALVSRAASVAVKRRSMKKFRELHAASEKNMTTVTLDDDEPSEDAGETPVPDSKGEETAKDAEAEQKVDGDKETSAKDKSEGDSPNIETPKKATNGNSSIKSPQKTPKKSAEKPTDAAMDVDNVAPEEPKKAVEQEVDSSSSNDEYYEPTLAELTNFLDNPPEEFADPNFCLTLIDFVDAIKVMQPSAKREGFITVPDTTWDDIGALEKIREELKLAVLAPVKYPEMLERLGLTAPSGVLLCGPPGCGKTLLAKAIANEAGINFISVKGPELMNMYVGESERAVRACFQRARNSAPCVIFFDEFDSLCPKRSDGGDGNNSGTRIVNQLLTEMDGVEERKGVYILAATNRPDIIDPAILRPGRLDTILYVGFPEQSERTEILKATTKNGKRPVLADDVDLDEIAAQTEGYTGADLAGLVKQASMFSLRQSLNNGDTNLDDLCVRSQHFQEALQQLRPSVNEQDRKIYDKLRLKYAAPRVPTLNDK

Target ORF information:

RefSeq Version	NM_001169900.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster smallminded (smid), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169900.2

ORF Insert Sequence:

ATGAAGAAAG CCAAGCCGCT GCTGCACGAC CATCTAATAA CGATCCGAGT AAAGAAGTAT 
CTGGAGGAGC ACATCGGGGA AACCTACCTG GATGTGAAAC AGATGACCAG GGAGTTGATG 
CAAAAGTATC CGGAATATTC ACGACGCAAA TTCGGACCAT TCAGACAGCT GGTGCACCAA 
GCATTCTCGA TCATTTCGGA AAGCTATAAC TTGGACAAGG TAAGCAGTTC CGAGGAAGAT 
TGTGTTAGCG AGGACTCCGA GCCACCACCC ACGAATAGTG TGATGAACAA TATGATGAAC 
AGCCTGTATA GTCAACCAAG AAAGCCGCTG GCGCCCAAGC CAATCAGCGA GCCCATCGAT 
ATATCCAGTG GAGACGAGAA CGAAGATGAC TCCAATACAA AAACCACCAA CGGAGATGGG 
GTAGCTGCTG CTGCTGCTCC TCCTCCTCCT ACTCCAGCAG TGCAGGGCAG TGCTTTGAAA 
CGCCTGATGG AGGAAGTGCC GGAGATCGCA GTGGCTGCAA AGAAAGCTAA ACCGAATACT 
ATTCACGTTA GCAGTTCGGA GGCCATTCAA AAGCTGCACC AAGTTGTGGG CAACAGAGCG 
AAAAATCTTT CAGAGGACGC CGTTCCGCGT TCCAAAGATC ACCGTAATGT GCCTGGCTTA 
TATCAGCAGT TGCACCAAAA CCAAAGTCGC GATCGCCTTC GGAAGTTCAA AAGGGATCTG 
GAGGTGCAGC ACCCAACGGA AAGTTTCCGG GACATTGGTG GCATGGATAG CACTCTTAAG 
GAGTTGTGTG AAATGCTGAT CCATATTAAG TCGCCGGAGT TCTACTTTCA ATTGGGTCTA 
TTGCCTTCGA GAGGTTTATT GCTTCATGGG CCGCCAGGCT GTGGCAAAAC CTTTCTTGCT 
CGTGCCATTA GCGGGCAACT AAAAATGCCT CTAATGGAAA TTCCGGCCAC AGAGCTAATT 
GGTGGCATCT CTGGTGAGTC GGAAGAACGG ATTCGCGAGG TCTTTGACCA GGCCATTGGC 
TACTCGCCTT GTGTGCTTTT CATCGATGAA ATCGACGCCA TCGGAGGAAA TCGTCAGTGG 
GCGTCCAAGG ATATGGAACG TCGCATTGTC TCGCAGCTGA TAAGCAGTCT GGACAACCTG 
AAGGCCAACG AGTTTGGCCA ATCAGTAGTC GTCATAGCGG CCACCACGCG TCCCGATGTC 
CTCGACCCTG GACTACGTCG CATCGGTCGC TTCGATCACG AAATTGCCAT ACACATCCCA 
TCGCGGAAAG AGCGGCGTGA AATCTTGCGC ATCCAGTGCG AGGGCTTGTC CGTCGATCCC 
AAGCTAAATT ATGATAAGAT TGCAGAGCTA ACACCGGGAT ATGTCGGTGC TGATCTAATG 
GCTCTCGTAA GTCGTGCAGC TTCTGTGGCC GTAAAGCGTA GGTCCATGAA GAAGTTCCGT 
GAACTGCACG CTGCTAGTGA AAAGAATATG ACAACGGTCA CTTTGGATGA CGACGAGCCC 
AGCGAGGACG CAGGTGAAAC ACCTGTGCCT GATTCAAAAG GTGAAGAAAC TGCAAAAGAT 
GCAGAGGCAG AACAAAAGGT AGATGGTGAC AAGGAAACAT CTGCTAAGGA TAAATCAGAA 
GGCGACTCGC CAAATATTGA AACCCCCAAG AAAGCGACCA ACGGCAACTC ATCGATAAAG 
TCGCCGCAGA AAACACCGAA GAAATCAGCG GAGAAACCAA CTGACGCAGC GATGGATGTA 
GACAATGTTG CTCCCGAGGA GCCGAAAAAA GCCGTTGAGC AGGAGGTGGA TTCCTCCTCC 
TCCAACGACG AGTACTACGA ACCCACACTG GCCGAACTGA CCAATTTTCT TGACAACCCC 
CCTGAAGAGT TCGCCGACCC AAACTTTTGC CTCACTCTCA TTGATTTCGT AGATGCCATT 
AAGGTGATGC AGCCTTCGGC CAAGCGCGAG GGCTTTATAA CAGTACCAGA TACCACTTGG 
GATGACATTG GTGCCCTAGA GAAAATTCGC GAAGAGCTTA AGTTGGCTGT CTTGGCACCA 
GTTAAGTATC CAGAGATGCT GGAACGTCTT GGGCTGACAG CTCCGTCAGG CGTTCTGCTA 
TGCGGTCCGC CGGGTTGCGG CAAAACTCTT TTGGCCAAGG CTATTGCCAA TGAGGCTGGC 
ATCAATTTCA TATCGGTGAA GGGCCCCGAA CTTATGAACA TGTATGTCGG CGAGAGCGAA 
CGAGCTGTGC GTGCCTGTTT CCAACGTGCC CGGAACTCTG CACCCTGCGT CATCTTCTTC 
GACGAGTTCG ACTCCTTGTG CCCCAAGCGA TCGGATGGTG GCGATGGTAA TAATTCGGGC 
ACTCGTATCG TTAACCAACT GCTGACCGAA ATGGATGGCG TCGAGGAGCG CAAGGGTGTA 
TACATATTGG CGGCCACCAA CAGACCGGAT ATCATTGATC CGGCCATCCT GCGACCGGGT 
CGCCTGGACA CCATTTTGTA CGTGGGTTTC CCCGAGCAGA GCGAACGTAC CGAGATTTTG 
AAGGCCACCA CAAAGAACGG AAAGCGTCCG GTTCTGGCCG ATGATGTAGA TCTCGATGAA 
ATTGCTGCCC AAACCGAGGG CTATACTGGC GCAGATTTGG CAGGATTGGT CAAGCAGGCC 
TCCATGTTCT CCCTGCGACA ATCTCTAAAC AACGGCGACA CAAATCTCGA CGATTTGTGC 
GTACGCAGCC AGCATTTCCA GGAAGCTTTG CAGCAACTTC GGCCCTCAGT TAACGAACAG 
GACCGCAAAA TATACGATAA ACTGCGCCTG AAATATGCTG CACCAAGGGT ACCCACTCTA 
AATGATAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smid ( NM_001274584.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261513.1 Drosophila melanogaster smallminded (smid), transcript variant H, mRNA.

Price & Availability↑

CloneID	OFa17797
Clone ID Related Accession (Same CDS sequence)	NM_001274584.1
Accession Version	NM_001274584.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2784bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	smallminded, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGAAAG CCAAGCCGCT GCTGCACGAC CATCTAATAA CGATCCGAGT AAAGAAGTAT 
CTGGAGGAGC ACATCGGGGA AACCTACCTG GATGTGAAAC AGATGACCAG GGAGTTGATG 
CAAAAGTATC CGGAATATTC ACGACGCAAA TTCGGACCAT TCAGACAGCT GGTGCACCAA 
GCATTCTCGA TCATTTCGGA AAGCTATAAC TTGGACAAGG TAAGCAGTTC CGAGGAAGAT 
TGTGTTAGCG AGGACTCCGA GCCACCACCC ACGAATAGTG TGATGAACAA TATGATGAAC 
AGCCTGTATA GTCAACCAAG AAAGCCGCTG GCGCCCAAGC CAATCAGCGA GCCCATCGAT 
ATATCCAGTG GAGACGAGAA CGAAGATGAC TCCAATACAA AAACCACCAA CGGAGATGGG 
GTAGCTGCTG CTGCTGCTCC TCCTCCTCCT ACTCCAGCAG TGCAGGGCAG TGCTTTGAAA 
CGCCTGATGG AGGAAGTGCC GGAGATCGCA GTGGCTGCAA AGAAAGTGCA CCAAGTTGTG 
GGCAACAGAG CGAAAAATCT TTCAGAGGAC GCCGTTCCGC GTTCCAAAGA TCACCGTAAT 
GTGCCTGGCT TATATCAGCA GTTGCACCAA AACCAAAGTC GCGATCGCCT TCGGAAGTTC 
AAAAGGGATC TGGAGGTGCA GCACCCAACG GAAAGTTTCC GGGACATTGG TGGCATGGAT 
AGCACTCTTA AGGAGTTGTG TGAAATGCTG ATCCATATTA AGTCGCCGGA GTTCTACTTT 
CAATTGGGTC TATTGCCTTC GAGAGGTTTA TTGCTTCATG GGCCGCCAGG CTGTGGCAAA 
ACCTTTCTTG CTCGTGCCAT TAGCGGGCAA CTAAAAATGC CTCTAATGGA AATTCCGGCC 
ACAGAGCTAA TTGGTGGCAT CTCTGGTGAG TCGGAAGAAC GGATTCGCGA GGTCTTTGAC 
CAGGCCATTG GCTACTCGCC TTGTGTGCTT TTCATCGATG AAATCGACGC CATCGGAGGA 
AATCGTCAGT GGGCGTCCAA GGATATGGAA CGTCGCATTG TCTCGCAGCT GATAAGCAGT 
CTGGACAACC TGAAGGCCAA CGAGTTTGGC CAATCAGTAG TCGTCATAGC GGCCACCACG 
CGTCCCGATG TCCTCGACCC TGGACTACGT CGCATCGGTC GCTTCGATCA CGAAATTGCC 
ATACACATCC CATCGCGGAA AGAGCGGCGT GAAATCTTGC GCATCCAGTG CGAGGGCTTG 
TCCGTCGATC CCAAGCTAAA TTATGATAAG ATTGCAGAGC TAACACCGGG ATATGTCGGT 
GCTGATCTAA TGGCTCTCGT AAGTCGTGCA GCTTCTGTGG CCGTAAAGCG TAGGTCCATG 
AAGAAGTTCC GTGAACTGCA CGCTGCTAGT GAAAAGAATA TGACAACGGT CACTTTGGAT 
GACGACGAGC CCAGCGAGGA CGCAGGTGAA ACACCTGTGC CTGATTCAAA AGGTGAAGAA 
ACTGCAAAAG ATGCAGAGGC AGAACAAAAG GTAGATGGTG ACAAGGAAAC ATCTGCTAAG 
GATAAATCAG AAGGCGACTC GCCAAATATT GAAACCCCCA AGAAAGCGAC CAACGGCAAC 
TCATCGATAA AGTCGCCGCA GAAAACACCG AAGAAATCAG CGGAGAAACC AACTGACGCA 
GCGATGGATG TAGACAATGT TGCTCCCGAG GAGCCGAAAA AAGCCGTTGA GCAGGAGGTG 
GATTCCTCCT CCTCCAACGA CGAGTACTAC GAACCCACAC TGGCCGAACT GACCAATTTT 
CTTGACAACC CCCCTGAAGA GTTCGCCGAC CCAAACTTTT GCCTCACTCT CATTGATTTC 
GTAGATGCCA TTAAGGTGAT GCAGCCTTCG GCCAAGCGCG AGGGCTTTAT AACAGTACCA 
GATACCACTT GGGATGACAT TGGTGCCCTA GAGAAAATTC GCGAAGAGCT TAAGTTGGCT 
GTCTTGGCAC CAGTTAAGTA TCCAGAGATG CTGGAACGTC TTGGGCTGAC AGCTCCGTCA 
GGCGTTCTGC TATGCGGTCC GCCGGGTTGC GGCAAAACTC TTTTGGCCAA GGCTATTGCC 
AATGAGGCTG GCATCAATTT CATATCGGTG AAGGGCCCCG AACTTATGAA CATGTATGTC 
GGCGAGAGCG AACGAGCTGT GCGTGCCTGT TTCCAACGTG CCCGGAACTC TGCACCCTGC 
GTCATCTTCT TCGACGAGTT CGACTCCTTG TGCCCCAAGC GATCGGATGG TGGCGATGGT 
AATAATTCGG GCACTCGTAT CGTTAACCAA CTGCTGACCG AAATGGATGG CGTCGAGGAG 
CGCAAGGGTG TATACATATT GGCGGCCACC AACAGACCGG ATATCATTGA TCCGGCCATC 
CTGCGACCGG GTCGCCTGGA CACCATTTTG TACGTGGGTT TCCCCGAGCA GAGCGAACGT 
ACCGAGATTT TGAAGGCCAC CACAAAGAAC GGAAAGCGTC CGGTTCTGGC CGATGATGTA 
GATCTCGATG AAATTGCTGC CCAAACCGAG GGCTATACTG GCGCAGATTT GGCAGGATTG 
GTCAAGCAGG CCTCCATGTT CTCCCTGCGA CAATCTCTAA ACAACGGCGA CACAAATCTC 
GACGATTTGT GCGTACGCAG CCAGCATTTC CAGGAAGCTT TGCAGCAACT TCGGCCCTCA 
GTTAACGAAC AGGACCGCAA AATATACGAT AAACTGCGCC TGAAATATGC TGCACCAAGG 
GTACCCACTC TAAATGATAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261513.1
CDS	107..2890
Translation	MKKAKPLLHDHLITIRVKKYLEEHIGETYLDVKQMTRELMQKYPEYSRRKFGPFRQLVHQAFSIISESYNLDKVSSSEEDCVSEDSEPPPTNSVMNNMMNSLYSQPRKPLAPKPISEPIDISSGDENEDDSNTKTTNGDGVAAAAAPPPPTPAVQGSALKRLMEEVPEIAVAAKKVHQVVGNRAKNLSEDAVPRSKDHRNVPGLYQQLHQNQSRDRLRKFKRDLEVQHPTESFRDIGGMDSTLKELCEMLIHIKSPEFYFQLGLLPSRGLLLHGPPGCGKTFLARAISGQLKMPLMEIPATELIGGISGESEERIREVFDQAIGYSPCVLFIDEIDAIGGNRQWASKDMERRIVSQLISSLDNLKANEFGQSVVVIAATTRPDVLDPGLRRIGRFDHEIAIHIPSRKERREILRIQCEGLSVDPKLNYDKIAELTPGYVGADLMALVSRAASVAVKRRSMKKFRELHAASEKNMTTVTLDDDEPSEDAGETPVPDSKGEETAKDAEAEQKVDGDKETSAKDKSEGDSPNIETPKKATNGNSSIKSPQKTPKKSAEKPTDAAMDVDNVAPEEPKKAVEQEVDSSSSNDEYYEPTLAELTNFLDNPPEEFADPNFCLTLIDFVDAIKVMQPSAKREGFITVPDTTWDDIGALEKIREELKLAVLAPVKYPEMLERLGLTAPSGVLLCGPPGCGKTLLAKAIANEAGINFISVKGPELMNMYVGESERAVRACFQRARNSAPCVIFFDEFDSLCPKRSDGGDGNNSGTRIVNQLLTEMDGVEERKGVYILAATNRPDIIDPAILRPGRLDTILYVGFPEQSERTEILKATTKNGKRPVLADDVDLDEIAAQTEGYTGADLAGLVKQASMFSLRQSLNNGDTNLDDLCVRSQHFQEALQQLRPSVNEQDRKIYDKLRLKYAAPRVPTLNDK

Target ORF information:

RefSeq Version	NM_001274584.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster smallminded (smid), transcript variant H, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274584.1

ORF Insert Sequence:

ATGAAGAAAG CCAAGCCGCT GCTGCACGAC CATCTAATAA CGATCCGAGT AAAGAAGTAT 
CTGGAGGAGC ACATCGGGGA AACCTACCTG GATGTGAAAC AGATGACCAG GGAGTTGATG 
CAAAAGTATC CGGAATATTC ACGACGCAAA TTCGGACCAT TCAGACAGCT GGTGCACCAA 
GCATTCTCGA TCATTTCGGA AAGCTATAAC TTGGACAAGG TAAGCAGTTC CGAGGAAGAT 
TGTGTTAGCG AGGACTCCGA GCCACCACCC ACGAATAGTG TGATGAACAA TATGATGAAC 
AGCCTGTATA GTCAACCAAG AAAGCCGCTG GCGCCCAAGC CAATCAGCGA GCCCATCGAT 
ATATCCAGTG GAGACGAGAA CGAAGATGAC TCCAATACAA AAACCACCAA CGGAGATGGG 
GTAGCTGCTG CTGCTGCTCC TCCTCCTCCT ACTCCAGCAG TGCAGGGCAG TGCTTTGAAA 
CGCCTGATGG AGGAAGTGCC GGAGATCGCA GTGGCTGCAA AGAAAGTGCA CCAAGTTGTG 
GGCAACAGAG CGAAAAATCT TTCAGAGGAC GCCGTTCCGC GTTCCAAAGA TCACCGTAAT 
GTGCCTGGCT TATATCAGCA GTTGCACCAA AACCAAAGTC GCGATCGCCT TCGGAAGTTC 
AAAAGGGATC TGGAGGTGCA GCACCCAACG GAAAGTTTCC GGGACATTGG TGGCATGGAT 
AGCACTCTTA AGGAGTTGTG TGAAATGCTG ATCCATATTA AGTCGCCGGA GTTCTACTTT 
CAATTGGGTC TATTGCCTTC GAGAGGTTTA TTGCTTCATG GGCCGCCAGG CTGTGGCAAA 
ACCTTTCTTG CTCGTGCCAT TAGCGGGCAA CTAAAAATGC CTCTAATGGA AATTCCGGCC 
ACAGAGCTAA TTGGTGGCAT CTCTGGTGAG TCGGAAGAAC GGATTCGCGA GGTCTTTGAC 
CAGGCCATTG GCTACTCGCC TTGTGTGCTT TTCATCGATG AAATCGACGC CATCGGAGGA 
AATCGTCAGT GGGCGTCCAA GGATATGGAA CGTCGCATTG TCTCGCAGCT GATAAGCAGT 
CTGGACAACC TGAAGGCCAA CGAGTTTGGC CAATCAGTAG TCGTCATAGC GGCCACCACG 
CGTCCCGATG TCCTCGACCC TGGACTACGT CGCATCGGTC GCTTCGATCA CGAAATTGCC 
ATACACATCC CATCGCGGAA AGAGCGGCGT GAAATCTTGC GCATCCAGTG CGAGGGCTTG 
TCCGTCGATC CCAAGCTAAA TTATGATAAG ATTGCAGAGC TAACACCGGG ATATGTCGGT 
GCTGATCTAA TGGCTCTCGT AAGTCGTGCA GCTTCTGTGG CCGTAAAGCG TAGGTCCATG 
AAGAAGTTCC GTGAACTGCA CGCTGCTAGT GAAAAGAATA TGACAACGGT CACTTTGGAT 
GACGACGAGC CCAGCGAGGA CGCAGGTGAA ACACCTGTGC CTGATTCAAA AGGTGAAGAA 
ACTGCAAAAG ATGCAGAGGC AGAACAAAAG GTAGATGGTG ACAAGGAAAC ATCTGCTAAG 
GATAAATCAG AAGGCGACTC GCCAAATATT GAAACCCCCA AGAAAGCGAC CAACGGCAAC 
TCATCGATAA AGTCGCCGCA GAAAACACCG AAGAAATCAG CGGAGAAACC AACTGACGCA 
GCGATGGATG TAGACAATGT TGCTCCCGAG GAGCCGAAAA AAGCCGTTGA GCAGGAGGTG 
GATTCCTCCT CCTCCAACGA CGAGTACTAC GAACCCACAC TGGCCGAACT GACCAATTTT 
CTTGACAACC CCCCTGAAGA GTTCGCCGAC CCAAACTTTT GCCTCACTCT CATTGATTTC 
GTAGATGCCA TTAAGGTGAT GCAGCCTTCG GCCAAGCGCG AGGGCTTTAT AACAGTACCA 
GATACCACTT GGGATGACAT TGGTGCCCTA GAGAAAATTC GCGAAGAGCT TAAGTTGGCT 
GTCTTGGCAC CAGTTAAGTA TCCAGAGATG CTGGAACGTC TTGGGCTGAC AGCTCCGTCA 
GGCGTTCTGC TATGCGGTCC GCCGGGTTGC GGCAAAACTC TTTTGGCCAA GGCTATTGCC 
AATGAGGCTG GCATCAATTT CATATCGGTG AAGGGCCCCG AACTTATGAA CATGTATGTC 
GGCGAGAGCG AACGAGCTGT GCGTGCCTGT TTCCAACGTG CCCGGAACTC TGCACCCTGC 
GTCATCTTCT TCGACGAGTT CGACTCCTTG TGCCCCAAGC GATCGGATGG TGGCGATGGT 
AATAATTCGG GCACTCGTAT CGTTAACCAA CTGCTGACCG AAATGGATGG CGTCGAGGAG 
CGCAAGGGTG TATACATATT GGCGGCCACC AACAGACCGG ATATCATTGA TCCGGCCATC 
CTGCGACCGG GTCGCCTGGA CACCATTTTG TACGTGGGTT TCCCCGAGCA GAGCGAACGT 
ACCGAGATTT TGAAGGCCAC CACAAAGAAC GGAAAGCGTC CGGTTCTGGC CGATGATGTA 
GATCTCGATG AAATTGCTGC CCAAACCGAG GGCTATACTG GCGCAGATTT GGCAGGATTG 
GTCAAGCAGG CCTCCATGTT CTCCCTGCGA CAATCTCTAA ACAACGGCGA CACAAATCTC 
GACGATTTGT GCGTACGCAG CCAGCATTTC CAGGAAGCTT TGCAGCAACT TCGGCCCTCA 
GTTAACGAAC AGGACCGCAA AATATACGAT AAACTGCGCC TGAAATATGC TGCACCAAGG 
GTACCCACTC TAAATGATAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smid ( NM_079235.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523959.2 Drosophila melanogaster smallminded (smid), transcript variant A, mRNA.

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CloneID	OFa20646
Clone ID Related Accession (Same CDS sequence)	NM_079235.4
Accession Version	NM_079235.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2835bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	smallminded, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079235.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGAAAG CCAAGCCGCT GCTGCACGAC CATCTAATAA CGATCCGAGT AAAGAAGTAT 
CTGGAGGAGC ACATCGGGGA AACCTACCTG GATGTGAAAC AGATGACCAG GGAGTTGATG 
CAAAAGTATC CGGAATATTC ACGACGCAAA TTCGGACCAT TCAGACAGCT GGTGCACCAA 
GCATTCTCGA TCATTTCGGA AAGCTATAAC TTGGACAAGG TAAGCAGTTC CGAGGAAGAT 
TGTGTTAGCG AGGACTCCGA GCCACCACCC ACGAATAGTG TGATGAACAA TATGATGAAC 
AGCCTGTATA GTCAACCAAG AAAGCCGCTG GCGCCCAAGC CAATCAGCGA GCCCATCGAT 
ATATCCAGTG GAGACGAGAA CGAAGATGAC TCCAATACAA AAACCACCAA CGGAGATGGG 
GTAGCTGCTG CTGCTGCTCC TCCTCCTCCT ACTCCAGCAG TGCAGGGCAG TGCTTTGAAA 
CGCCTGATGG AGGAAGTGCC GGAGATCGCA GTGGCTGCAA AGAAAGTAGC TAAACCGAAT 
ACTATTCACG TTAGCAGTTC GGAGGCCATT CAAAAGCTGC ACCAAGTTGT GGGCAACAGA 
GCGAAAAATC TTTCAGAGGA CGCCGTTCCG CGTTCCAAAG ATCACCGTAA TGTGCCTGGC 
TTATATCAGC AGTTGCACCA AAACCAAAGT CGCGATCGCC TTCGGAAGTT CAAAAGGGAT 
CTGGAGGTGC AGCACCCAAC GGAAAGTTTC CGGGACATTG GTGGCATGGA TAGCACTCTT 
AAGGAGTTGT GTGAAATGCT GATCCATATT AAGTCGCCGG AGTTCTACTT TCAATTGGGT 
CTATTGCCTT CGAGAGGTTT ATTGCTTCAT GGGCCGCCAG GCTGTGGCAA AACCTTTCTT 
GCTCGTGCCA TTAGCGGGCA ACTAAAAATG CCTCTAATGG AAATTCCGGC CACAGAGCTA 
ATTGGTGGCA TCTCTGGTGA GTCGGAAGAA CGGATTCGCG AGGTCTTTGA CCAGGCCATT 
GGCTACTCGC CTTGTGTGCT TTTCATCGAT GAAATCGACG CCATCGGAGG AAATCGTCAG 
TGGGCGTCCA AGGATATGGA ACGTCGCATT GTCTCGCAGC TGATAAGCAG TCTGGACAAC 
CTGAAGGCCA ACGAGTTTGG CCAATCAGTA GTCGTCATAG CGGCCACCAC GCGTCCCGAT 
GTCCTCGACC CTGGACTACG TCGCATCGGT CGCTTCGATC ACGAAATTGC CATACACATC 
CCATCGCGGA AAGAGCGGCG TGAAATCTTG CGCATCCAGT GCGAGGGCTT GTCCGTCGAT 
CCCAAGCTAA ATTATGATAA GATTGCAGAG CTAACACCGG GATATGTCGG TGCTGATCTA 
ATGGCTCTCG TAAGTCGTGC AGCTTCTGTG GCCGTAAAGC GTAGGTCCAT GAAGAAGTTC 
CGTGAACTGC ACGCTGCTAG TGAAAAGAAT ATGACAACGG TCACTTTGGA TGACGACGAG 
CCCAGCGAGG ACGCAGGTGA AACACCTGTG CCTGATTCAA AAGGTGAAGA AACTGCAAAA 
GATGCAGAGG CAGAACAAAA GGTAGATGGT GACAAGGAAA CATCTGCTAA GGATAAATCA 
GAAGGCGACT CGCCAAATAT TGAAACCCCC AAGAAAGCGA CCAACGGCAA CTCATCGATA 
AAGTCGCCGC AGAAAACACC GAAGAAATCA GCGGAGAAAC CAACTGACGC AGCGATGGAT 
GTAGACAATG TTGCTCCCGA GGAGCCGAAA AAAGCCGTTG AGCAGGAGGT GGATTCCTCC 
TCCTCCAACG ACGAGTACTA CGAACCCACA CTGGCCGAAC TGACCAATTT TCTTGACAAC 
CCCCCTGAAG AGTTCGCCGA CCCAAACTTT TGCCTCACTC TCATTGATTT CGTAGATGCC 
ATTAAGGTGA TGCAGCCTTC GGCCAAGCGC GAGGGCTTTA TAACAGTACC AGATACCACT 
TGGGATGACA TTGGTGCCCT AGAGAAAATT CGCGAAGAGC TTAAGTTGGC TGTCTTGGCA 
CCAGTTAAGT ATCCAGAGAT GCTGGAACGT CTTGGGCTGA CAGCTCCGTC AGGCGTTCTG 
CTATGCGGTC CGCCGGGTTG CGGCAAAACT CTTTTGGCCA AGGCTATTGC CAATGAGGCT 
GGCATCAATT TCATATCGGT GAAGGGCCCC GAACTTATGA ACATGTATGT CGGCGAGAGC 
GAACGAGCTG TGCGTGCCTG TTTCCAACGT GCCCGGAACT CTGCACCCTG CGTCATCTTC 
TTCGACGAGT TCGACTCCTT GTGCCCCAAG CGATCGGATG GTGGCGATGG TAATAATTCG 
GGCACTCGTA TCGTTAACCA ACTGCTGACC GAAATGGATG GCGTCGAGGA GCGCAAGGGT 
GTATACATAT TGGCGGCCAC CAACAGACCG GATATCATTG ATCCGGCCAT CCTGCGACCG 
GGTCGCCTGG ACACCATTTT GTACGTGGGT TTCCCCGAGC AGAGCGAACG TACCGAGATT 
TTGAAGGCCA CCACAAAGAA CGGAAAGCGT CCGGTTCTGG CCGATGATGT AGATCTCGAT 
GAAATTGCTG CCCAAACCGA GGGCTATACT GGCGCAGATT TGGCAGGATT GGTCAAGCAG 
GCCTCCATGT TCTCCCTGCG ACAATCTCTA AACAACGGCG ACACAAATCT CGACGATTTG 
TGCGTACGCA GCCAGCATTT CCAGGAAGCT TTGCAGCAAC TTCGGCCCTC AGTTAACGAA 
CAGGACCGCA AAATATACGA TAAACTGCGC CTGAAATATG CTGCACCAAG GGTACCCACT 
CTAAATGATA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523959.2
CDS	107..2941
Translation	MKKAKPLLHDHLITIRVKKYLEEHIGETYLDVKQMTRELMQKYPEYSRRKFGPFRQLVHQAFSIISESYNLDKVSSSEEDCVSEDSEPPPTNSVMNNMMNSLYSQPRKPLAPKPISEPIDISSGDENEDDSNTKTTNGDGVAAAAAPPPPTPAVQGSALKRLMEEVPEIAVAAKKVAKPNTIHVSSSEAIQKLHQVVGNRAKNLSEDAVPRSKDHRNVPGLYQQLHQNQSRDRLRKFKRDLEVQHPTESFRDIGGMDSTLKELCEMLIHIKSPEFYFQLGLLPSRGLLLHGPPGCGKTFLARAISGQLKMPLMEIPATELIGGISGESEERIREVFDQAIGYSPCVLFIDEIDAIGGNRQWASKDMERRIVSQLISSLDNLKANEFGQSVVVIAATTRPDVLDPGLRRIGRFDHEIAIHIPSRKERREILRIQCEGLSVDPKLNYDKIAELTPGYVGADLMALVSRAASVAVKRRSMKKFRELHAASEKNMTTVTLDDDEPSEDAGETPVPDSKGEETAKDAEAEQKVDGDKETSAKDKSEGDSPNIETPKKATNGNSSIKSPQKTPKKSAEKPTDAAMDVDNVAPEEPKKAVEQEVDSSSSNDEYYEPTLAELTNFLDNPPEEFADPNFCLTLIDFVDAIKVMQPSAKREGFITVPDTTWDDIGALEKIREELKLAVLAPVKYPEMLERLGLTAPSGVLLCGPPGCGKTLLAKAIANEAGINFISVKGPELMNMYVGESERAVRACFQRARNSAPCVIFFDEFDSLCPKRSDGGDGNNSGTRIVNQLLTEMDGVEERKGVYILAATNRPDIIDPAILRPGRLDTILYVGFPEQSERTEILKATTKNGKRPVLADDVDLDEIAAQTEGYTGADLAGLVKQASMFSLRQSLNNGDTNLDDLCVRSQHFQEALQQLRPSVNEQDRKIYDKLRLKYAAPRVPTLNDK

Target ORF information:

RefSeq Version	NM_079235.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster smallminded (smid), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079235.4

ORF Insert Sequence:

ATGAAGAAAG CCAAGCCGCT GCTGCACGAC CATCTAATAA CGATCCGAGT AAAGAAGTAT 
CTGGAGGAGC ACATCGGGGA AACCTACCTG GATGTGAAAC AGATGACCAG GGAGTTGATG 
CAAAAGTATC CGGAATATTC ACGACGCAAA TTCGGACCAT TCAGACAGCT GGTGCACCAA 
GCATTCTCGA TCATTTCGGA AAGCTATAAC TTGGACAAGG TAAGCAGTTC CGAGGAAGAT 
TGTGTTAGCG AGGACTCCGA GCCACCACCC ACGAATAGTG TGATGAACAA TATGATGAAC 
AGCCTGTATA GTCAACCAAG AAAGCCGCTG GCGCCCAAGC CAATCAGCGA GCCCATCGAT 
ATATCCAGTG GAGACGAGAA CGAAGATGAC TCCAATACAA AAACCACCAA CGGAGATGGG 
GTAGCTGCTG CTGCTGCTCC TCCTCCTCCT ACTCCAGCAG TGCAGGGCAG TGCTTTGAAA 
CGCCTGATGG AGGAAGTGCC GGAGATCGCA GTGGCTGCAA AGAAAGTAGC TAAACCGAAT 
ACTATTCACG TTAGCAGTTC GGAGGCCATT CAAAAGCTGC ACCAAGTTGT GGGCAACAGA 
GCGAAAAATC TTTCAGAGGA CGCCGTTCCG CGTTCCAAAG ATCACCGTAA TGTGCCTGGC 
TTATATCAGC AGTTGCACCA AAACCAAAGT CGCGATCGCC TTCGGAAGTT CAAAAGGGAT 
CTGGAGGTGC AGCACCCAAC GGAAAGTTTC CGGGACATTG GTGGCATGGA TAGCACTCTT 
AAGGAGTTGT GTGAAATGCT GATCCATATT AAGTCGCCGG AGTTCTACTT TCAATTGGGT 
CTATTGCCTT CGAGAGGTTT ATTGCTTCAT GGGCCGCCAG GCTGTGGCAA AACCTTTCTT 
GCTCGTGCCA TTAGCGGGCA ACTAAAAATG CCTCTAATGG AAATTCCGGC CACAGAGCTA 
ATTGGTGGCA TCTCTGGTGA GTCGGAAGAA CGGATTCGCG AGGTCTTTGA CCAGGCCATT 
GGCTACTCGC CTTGTGTGCT TTTCATCGAT GAAATCGACG CCATCGGAGG AAATCGTCAG 
TGGGCGTCCA AGGATATGGA ACGTCGCATT GTCTCGCAGC TGATAAGCAG TCTGGACAAC 
CTGAAGGCCA ACGAGTTTGG CCAATCAGTA GTCGTCATAG CGGCCACCAC GCGTCCCGAT 
GTCCTCGACC CTGGACTACG TCGCATCGGT CGCTTCGATC ACGAAATTGC CATACACATC 
CCATCGCGGA AAGAGCGGCG TGAAATCTTG CGCATCCAGT GCGAGGGCTT GTCCGTCGAT 
CCCAAGCTAA ATTATGATAA GATTGCAGAG CTAACACCGG GATATGTCGG TGCTGATCTA 
ATGGCTCTCG TAAGTCGTGC AGCTTCTGTG GCCGTAAAGC GTAGGTCCAT GAAGAAGTTC 
CGTGAACTGC ACGCTGCTAG TGAAAAGAAT ATGACAACGG TCACTTTGGA TGACGACGAG 
CCCAGCGAGG ACGCAGGTGA AACACCTGTG CCTGATTCAA AAGGTGAAGA AACTGCAAAA 
GATGCAGAGG CAGAACAAAA GGTAGATGGT GACAAGGAAA CATCTGCTAA GGATAAATCA 
GAAGGCGACT CGCCAAATAT TGAAACCCCC AAGAAAGCGA CCAACGGCAA CTCATCGATA 
AAGTCGCCGC AGAAAACACC GAAGAAATCA GCGGAGAAAC CAACTGACGC AGCGATGGAT 
GTAGACAATG TTGCTCCCGA GGAGCCGAAA AAAGCCGTTG AGCAGGAGGT GGATTCCTCC 
TCCTCCAACG ACGAGTACTA CGAACCCACA CTGGCCGAAC TGACCAATTT TCTTGACAAC 
CCCCCTGAAG AGTTCGCCGA CCCAAACTTT TGCCTCACTC TCATTGATTT CGTAGATGCC 
ATTAAGGTGA TGCAGCCTTC GGCCAAGCGC GAGGGCTTTA TAACAGTACC AGATACCACT 
TGGGATGACA TTGGTGCCCT AGAGAAAATT CGCGAAGAGC TTAAGTTGGC TGTCTTGGCA 
CCAGTTAAGT ATCCAGAGAT GCTGGAACGT CTTGGGCTGA CAGCTCCGTC AGGCGTTCTG 
CTATGCGGTC CGCCGGGTTG CGGCAAAACT CTTTTGGCCA AGGCTATTGC CAATGAGGCT 
GGCATCAATT TCATATCGGT GAAGGGCCCC GAACTTATGA ACATGTATGT CGGCGAGAGC 
GAACGAGCTG TGCGTGCCTG TTTCCAACGT GCCCGGAACT CTGCACCCTG CGTCATCTTC 
TTCGACGAGT TCGACTCCTT GTGCCCCAAG CGATCGGATG GTGGCGATGG TAATAATTCG 
GGCACTCGTA TCGTTAACCA ACTGCTGACC GAAATGGATG GCGTCGAGGA GCGCAAGGGT 
GTATACATAT TGGCGGCCAC CAACAGACCG GATATCATTG ATCCGGCCAT CCTGCGACCG 
GGTCGCCTGG ACACCATTTT GTACGTGGGT TTCCCCGAGC AGAGCGAACG TACCGAGATT 
TTGAAGGCCA CCACAAAGAA CGGAAAGCGT CCGGTTCTGG CCGATGATGT AGATCTCGAT 
GAAATTGCTG CCCAAACCGA GGGCTATACT GGCGCAGATT TGGCAGGATT GGTCAAGCAG 
GCCTCCATGT TCTCCCTGCG ACAATCTCTA AACAACGGCG ACACAAATCT CGACGATTTG 
TGCGTACGCA GCCAGCATTT CCAGGAAGCT TTGCAGCAAC TTCGGCCCTC AGTTAACGAA 
CAGGACCGCA AAATATACGA TAAACTGCGC CTGAAATATG CTGCACCAAG GGTACCCACT 
CTAAATGATA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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smid cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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