Cdc27 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cdc27
Entrez Gene ID	38798
Full Name	Cell division cycle 27
Synonyms	APC3,Apc3,CDC27,CDC27Dm,CG8610,Dmcdc27,Dmel\CG8610,cdc27,l(3)L7123,mks
General protein information	Preferred Names Cell division cycle 27 Names CG8610 gene product from transcript CG8610-RB CG8610-PA CG8610-PB Cdc27-PA Cdc27-PB cell division cycle 27 cell division cycle 27 ortholog makos
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 65E9-65E9

mRNA and Protein(s)

mRNA	Protein	Name
NM_001274574.1	NP_001261503.1	cell division cycle 27, isoform B
NM_139836.3	NP_648093.2	cell division cycle 27, isoform A

Pathways from BioSystems

Homologies

	CDC27	XP_001115976.2
Canis lupus familiaris (dog)	CDC27	XP_548047.2
Rattus norvegicus (Norway rat)	Cdc27	NP_001019964.1
Homo sapiens (human)	CDC27	NP_001107563.1
Bos taurus (cattle)	CDC27	NP_001098898.1
Gallus gallus (chicken)	CDC27	NP_001006331.1
Danio rerio (zebrafish)	cdc27	NP_958857.1
Mus musculus (house mouse)	Cdc27	NP_663411.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007690	XP_308181.4
Oryza sativa (rice)	Os06g0622500	NP_001058099.2
Xenopus tropicalis (tropical clawed frog)	cdc27	NP_001072470.1
Pan troglodytes (chimpanzee)	CDC27	XP_511624.2
Drosophila melanogaster (fruit fly)	Cdc27	NP_001261503.1
Arabidopsis thaliana (thale cress)	HBT	NP_849994.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results show that the two potential Cdk1 phosphorylation sites on Drosophila Cdc27 are required for its chromosomal localisation during mitosis and imply that these localisations specific to Cdc27 are crucial for APC/C functions.
Title: Cdk1 phosphorylation sites on Cdc27 are required for correct chromosomal localisation and APC/C function in syncytial Drosophila embryos.

The following Cdc27 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cdc27 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa17776	NM_001274574.1 Latest version!	Drosophila melanogaster cell division cycle 27, transcript variant B (Cdc27), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa17776	NM_139836.3 Latest version!	Drosophila melanogaster cell division cycle 27, transcript variant A (Cdc27), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cdc27 ( NM_001274574.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261503.1 Drosophila melanogaster cell division cycle 27, transcript variant B (Cdc27), mRNA.

Price & Availability↑

CloneID	OFa17776
Clone ID Related Accession (Same CDS sequence)	NM_001274574.1 , NM_139836.3
Accession Version	NM_001274574.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2703bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cell division cycle 27, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGATTC AAGAGCCCGT GCAGGCCGCC ATATGGCATT GCCTGAACTA CTACGACTTC 
AAGGATGCCG TTTTCCTGTC GGAACGCCTG TGCTCAGAAG TGGAGAGCGA CGAGACGATA 
TTCCTGCTGG CCACCAGCTA CTTCCGCTCC AATCAGGTGC ACCAGGCATA TTGGCTGCTC 
AAGGAGAAGG CACGCCGCTC GCCGCAGTGC CGCTTTCTGC AGGCCAAATG TGCCTACGAG 
CTGAAAAAAT ACGCTGAGGC GGAGAGTGCC CTGATCTCCA CGGGATTCGC GGACGCCAAG 
AACTGCGATG AGCTGCAGCG GGACTTCGGG GATCTGGCCT GCTTCGCCTA TCAGCTAATG 
GCCCAGATCT GCATGAGGAC CGAGCGCAAT AAACTGGCCG TTAGTGCTTT AAGGCGCGCC 
CTCAAACTGA ACCCCTTCAT GTGGCACGCA TTCGCGGATC TGTGCCTGCT GGGCCAGGAT 
ACAGATGCGG CGGCCATATT CCAAATCCAC AGCACAGATG TGTTCAATAC CTGCCAGGGC 
AGCAGCGTAA ATGCCAATGC AATGGTGCTG TTCGGAGCCG AGCAGCAGGG TCAACAGCAT 
CAGGAGCGCC AGTCGCTGAT CACCAACCTA AGCAATGTCT CCAACTACAT TCTGACCACG 
CCCGTGGATC AGCAGCAACA ACAGATCAAC CAGAATCAGA ACCAGCACCA CAACAGCAAC 
ATGGTGACGC CCATCAACAA TAATAATAAT AACAACAATC TCAATAGTTC GATCAGCATG 
CTGCGCGGGG GTTTGGTACA AAACTCCAGC ATGCTGGCGA TGCTGGAGGA CACACCTATG 
GGAGCTCCGC AGGACCCGGC CGGCCAGTAT CAGCAGAACC AGCAGCTCTA CGACATGAGC 
AGCGGTACTC CGTTTCGCAA ACAGTTCAAA TACCTATCGG CCATCTCACC GCCAACACCG 
AGCTTCGGCA TCATGCCTTT AACTAGTCCG TGCACCGGCA ACGATGGATC CTTCATTGGC 
AACCACACGC CCGTTATGAA TATAAGCTAT TCGCCGATGC CACAGATGTT GGTCGAGGTC 
AACCAGGAGC CGAAGATGAT GGGCAAAAAG CTAAAGACAC ATGTGGGCGG ACTCATCAAT 
CGCAAAGAGG GCAGCCTTAA TAAACCCGCC GTCTTTACGC AAGCCGGAAA CATAACGCCC 
CGCACTCCGA ACAACAATAA TGTGGGCAAC AATGGCAATA TGAATGTGCC GCCCAATCCG 
AATGCTGCAG TGCGTCGCAG TTCGCGGCTG TTCAGCAACT CGTATTCGGT GAAGGAGAAC 
AATAAATCGC CCAACATAGC CAACAAGTTC GTGCAGCCAC GTTCGCCGCC CCGCAAAGCC 
AAGTCACGGA TGACCAAGAT CTGCCTAAAT AACGAACTGA TCGAGGATAA GTCCCACCAC 
CTCTCCGAGA AGCGCAAGGA GAAGGTGGAG ACTATTACTT CCTCGGGAGC GAATAACAAC 
AGCGGTGGAC GCAGTGCCGC CGAGGAGGCC AAAGTACTGC TGAACAATAG CCTAAACAAT 
GCCCAGACGA TGGCCCACCA GCTGATGGGA CTCAAGAAAC AATCGGCCGA TGGGCTGATG 
GCCCTGCTGC GGGGTTTAGC TGAAGCCTAT CAGCTGCTCT CGAACTTCCA GTGCAAGGCG 
GCCATAAAGC AGCTGGAGAC GACGATACCA AAGCACCACC TGAACTCCAG CTGGGTGCAG 
TCGCTCATCG GACTGGCCAG GTACGAGATG CGTGAGTATG AGGCGGCGGT GGCGATATTT 
GAGACCATCC ACAAGACAGA GCCATGTCGC TTGGACTACA TGGAGATATA CTCGTCATCC 
CTGTGGCATT TGCAGCGCGA GGTTGAGCTG TCAGCTCTCG CCCAGGATCT GATTAACCAG 
GACAAAACCA GCCCGGTTAC CTGGTGTGTG TCCGGCAATT GCTTTTCGCT GCAAAAGGAG 
CACGAAACCG CGATCAAGTT CTTCAAGCGG GCTGTCCAAG TGGATCCAGA CTTCGTCTAC 
AGCTACACAC TGTTGGGCCA CGAACTCGTA CTCACCGAAG AATTTGATAA GGCCATGGAC 
TACTTCCGTG CAGCAGTGGT TAGAGATCCG CGGCATTACA ATGCTTGGTA CGGCATCGGC 
ACCATCTACT CCAAGCAGGA GAAATACGAG CTGGCTGAGA TTCACTACGT GAAGGCGCTG 
AAAATCAATC CACAGAACTC GGTTATTCTC GTGCACATCG GCGCCATGCA ATTCTACATG 
AAGAAGAAGG ATCTGTCGCT GCAGACGCTA AACACGGCCG CCACGCTGGA CCCCAAGAAC 
CCGCTGACGC GATTCCATCG CGGCTCCATC TACTTCTCGC TGGGCAAGTA CCAGGAGGCG 
CTGCGCGAAC TGGAGGAGCT CAAGGAAGTC GTGCCCAAGG AGTCGGTGGT GTTCTATCTG 
ATCGGCAAAA TCCACAAGAC GCTCGGCAAC ATGGATCTGG CGCTGATGCA CTTCAGCTGG 
GCCACCGACC TAGATCCCAA AGGTGCCAAC AATCAAATCA AAGATGCCTT CGACTCAATG 
GCTCATCCCA GCTGCTGTCC GGCCAACACT ACGGCCCTGG ACGTTGATCT GGAGCCCACT 
TCGGAGCGTT CCGATGACTC GACGCAGGCG CAGCAGGACG GAAGCTACGA CAGCGACTAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261503.1
CDS	660..3362
Translation	MMIQEPVQAAIWHCLNYYDFKDAVFLSERLCSEVESDETIFLLATSYFRSNQVHQAYWLLKEKARRSPQCRFLQAKCAYELKKYAEAESALISTGFADAKNCDELQRDFGDLACFAYQLMAQICMRTERNKLAVSALRRALKLNPFMWHAFADLCLLGQDTDAAAIFQIHSTDVFNTCQGSSVNANAMVLFGAEQQGQQHQERQSLITNLSNVSNYILTTPVDQQQQQINQNQNQHHNSNMVTPINNNNNNNNLNSSISMLRGGLVQNSSMLAMLEDTPMGAPQDPAGQYQQNQQLYDMSSGTPFRKQFKYLSAISPPTPSFGIMPLTSPCTGNDGSFIGNHTPVMNISYSPMPQMLVEVNQEPKMMGKKLKTHVGGLINRKEGSLNKPAVFTQAGNITPRTPNNNNVGNNGNMNVPPNPNAAVRRSSRLFSNSYSVKENNKSPNIANKFVQPRSPPRKAKSRMTKICLNNELIEDKSHHLSEKRKEKVETITSSGANNNSGGRSAAEEAKVLLNNSLNNAQTMAHQLMGLKKQSADGLMALLRGLAEAYQLLSNFQCKAAIKQLETTIPKHHLNSSWVQSLIGLARYEMREYEAAVAIFETIHKTEPCRLDYMEIYSSSLWHLQREVELSALAQDLINQDKTSPVTWCVSGNCFSLQKEHETAIKFFKRAVQVDPDFVYSYTLLGHELVLTEEFDKAMDYFRAAVVRDPRHYNAWYGIGTIYSKQEKYELAEIHYVKALKINPQNSVILVHIGAMQFYMKKKDLSLQTLNTAATLDPKNPLTRFHRGSIYFSLGKYQEALRELEELKEVVPKESVVFYLIGKIHKTLGNMDLALMHFSWATDLDPKGANNQIKDAFDSMAHPSCCPANTTALDVDLEPTSERSDDSTQAQQDGSYDSDY

Target ORF information:

RefSeq Version	NM_001274574.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cell division cycle 27, transcript variant B (Cdc27), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274574.1

ORF Insert Sequence:

ATGATGATTC AAGAGCCCGT GCAGGCCGCC ATATGGCATT GCCTGAACTA CTACGACTTC 
AAGGATGCCG TTTTCCTGTC GGAACGCCTG TGCTCAGAAG TGGAGAGCGA CGAGACGATA 
TTCCTGCTGG CCACCAGCTA CTTCCGCTCC AATCAGGTGC ACCAGGCATA TTGGCTGCTC 
AAGGAGAAGG CACGCCGCTC GCCGCAGTGC CGCTTTCTGC AGGCCAAATG TGCCTACGAG 
CTGAAAAAAT ACGCTGAGGC GGAGAGTGCC CTGATCTCCA CGGGATTCGC GGACGCCAAG 
AACTGCGATG AGCTGCAGCG GGACTTCGGG GATCTGGCCT GCTTCGCCTA TCAGCTAATG 
GCCCAGATCT GCATGAGGAC CGAGCGCAAT AAACTGGCCG TTAGTGCTTT AAGGCGCGCC 
CTCAAACTGA ACCCCTTCAT GTGGCACGCA TTCGCGGATC TGTGCCTGCT GGGCCAGGAT 
ACAGATGCGG CGGCCATATT CCAAATCCAC AGCACAGATG TGTTCAATAC CTGCCAGGGC 
AGCAGCGTAA ATGCCAATGC AATGGTGCTG TTCGGAGCCG AGCAGCAGGG TCAACAGCAT 
CAGGAGCGCC AGTCGCTGAT CACCAACCTA AGCAATGTCT CCAACTACAT TCTGACCACG 
CCCGTGGATC AGCAGCAACA ACAGATCAAC CAGAATCAGA ACCAGCACCA CAACAGCAAC 
ATGGTGACGC CCATCAACAA TAATAATAAT AACAACAATC TCAATAGTTC GATCAGCATG 
CTGCGCGGGG GTTTGGTACA AAACTCCAGC ATGCTGGCGA TGCTGGAGGA CACACCTATG 
GGAGCTCCGC AGGACCCGGC CGGCCAGTAT CAGCAGAACC AGCAGCTCTA CGACATGAGC 
AGCGGTACTC CGTTTCGCAA ACAGTTCAAA TACCTATCGG CCATCTCACC GCCAACACCG 
AGCTTCGGCA TCATGCCTTT AACTAGTCCG TGCACCGGCA ACGATGGATC CTTCATTGGC 
AACCACACGC CCGTTATGAA TATAAGCTAT TCGCCGATGC CACAGATGTT GGTCGAGGTC 
AACCAGGAGC CGAAGATGAT GGGCAAAAAG CTAAAGACAC ATGTGGGCGG ACTCATCAAT 
CGCAAAGAGG GCAGCCTTAA TAAACCCGCC GTCTTTACGC AAGCCGGAAA CATAACGCCC 
CGCACTCCGA ACAACAATAA TGTGGGCAAC AATGGCAATA TGAATGTGCC GCCCAATCCG 
AATGCTGCAG TGCGTCGCAG TTCGCGGCTG TTCAGCAACT CGTATTCGGT GAAGGAGAAC 
AATAAATCGC CCAACATAGC CAACAAGTTC GTGCAGCCAC GTTCGCCGCC CCGCAAAGCC 
AAGTCACGGA TGACCAAGAT CTGCCTAAAT AACGAACTGA TCGAGGATAA GTCCCACCAC 
CTCTCCGAGA AGCGCAAGGA GAAGGTGGAG ACTATTACTT CCTCGGGAGC GAATAACAAC 
AGCGGTGGAC GCAGTGCCGC CGAGGAGGCC AAAGTACTGC TGAACAATAG CCTAAACAAT 
GCCCAGACGA TGGCCCACCA GCTGATGGGA CTCAAGAAAC AATCGGCCGA TGGGCTGATG 
GCCCTGCTGC GGGGTTTAGC TGAAGCCTAT CAGCTGCTCT CGAACTTCCA GTGCAAGGCG 
GCCATAAAGC AGCTGGAGAC GACGATACCA AAGCACCACC TGAACTCCAG CTGGGTGCAG 
TCGCTCATCG GACTGGCCAG GTACGAGATG CGTGAGTATG AGGCGGCGGT GGCGATATTT 
GAGACCATCC ACAAGACAGA GCCATGTCGC TTGGACTACA TGGAGATATA CTCGTCATCC 
CTGTGGCATT TGCAGCGCGA GGTTGAGCTG TCAGCTCTCG CCCAGGATCT GATTAACCAG 
GACAAAACCA GCCCGGTTAC CTGGTGTGTG TCCGGCAATT GCTTTTCGCT GCAAAAGGAG 
CACGAAACCG CGATCAAGTT CTTCAAGCGG GCTGTCCAAG TGGATCCAGA CTTCGTCTAC 
AGCTACACAC TGTTGGGCCA CGAACTCGTA CTCACCGAAG AATTTGATAA GGCCATGGAC 
TACTTCCGTG CAGCAGTGGT TAGAGATCCG CGGCATTACA ATGCTTGGTA CGGCATCGGC 
ACCATCTACT CCAAGCAGGA GAAATACGAG CTGGCTGAGA TTCACTACGT GAAGGCGCTG 
AAAATCAATC CACAGAACTC GGTTATTCTC GTGCACATCG GCGCCATGCA ATTCTACATG 
AAGAAGAAGG ATCTGTCGCT GCAGACGCTA AACACGGCCG CCACGCTGGA CCCCAAGAAC 
CCGCTGACGC GATTCCATCG CGGCTCCATC TACTTCTCGC TGGGCAAGTA CCAGGAGGCG 
CTGCGCGAAC TGGAGGAGCT CAAGGAAGTC GTGCCCAAGG AGTCGGTGGT GTTCTATCTG 
ATCGGCAAAA TCCACAAGAC GCTCGGCAAC ATGGATCTGG CGCTGATGCA CTTCAGCTGG 
GCCACCGACC TAGATCCCAA AGGTGCCAAC AATCAAATCA AAGATGCCTT CGACTCAATG 
GCTCATCCCA GCTGCTGTCC GGCCAACACT ACGGCCCTGG ACGTTGATCT GGAGCCCACT 
TCGGAGCGTT CCGATGACTC GACGCAGGCG CAGCAGGACG GAAGCTACGA CAGCGACTAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc27 ( NM_139836.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648093.2 Drosophila melanogaster cell division cycle 27, transcript variant A (Cdc27), mRNA.

Price & Availability↑

CloneID	OFa17776
Clone ID Related Accession (Same CDS sequence)	NM_001274574.1 , NM_139836.3
Accession Version	NM_139836.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2703bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cell division cycle 27, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_139836.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGATTC AAGAGCCCGT GCAGGCCGCC ATATGGCATT GCCTGAACTA CTACGACTTC 
AAGGATGCCG TTTTCCTGTC GGAACGCCTG TGCTCAGAAG TGGAGAGCGA CGAGACGATA 
TTCCTGCTGG CCACCAGCTA CTTCCGCTCC AATCAGGTGC ACCAGGCATA TTGGCTGCTC 
AAGGAGAAGG CACGCCGCTC GCCGCAGTGC CGCTTTCTGC AGGCCAAATG TGCCTACGAG 
CTGAAAAAAT ACGCTGAGGC GGAGAGTGCC CTGATCTCCA CGGGATTCGC GGACGCCAAG 
AACTGCGATG AGCTGCAGCG GGACTTCGGG GATCTGGCCT GCTTCGCCTA TCAGCTAATG 
GCCCAGATCT GCATGAGGAC CGAGCGCAAT AAACTGGCCG TTAGTGCTTT AAGGCGCGCC 
CTCAAACTGA ACCCCTTCAT GTGGCACGCA TTCGCGGATC TGTGCCTGCT GGGCCAGGAT 
ACAGATGCGG CGGCCATATT CCAAATCCAC AGCACAGATG TGTTCAATAC CTGCCAGGGC 
AGCAGCGTAA ATGCCAATGC AATGGTGCTG TTCGGAGCCG AGCAGCAGGG TCAACAGCAT 
CAGGAGCGCC AGTCGCTGAT CACCAACCTA AGCAATGTCT CCAACTACAT TCTGACCACG 
CCCGTGGATC AGCAGCAACA ACAGATCAAC CAGAATCAGA ACCAGCACCA CAACAGCAAC 
ATGGTGACGC CCATCAACAA TAATAATAAT AACAACAATC TCAATAGTTC GATCAGCATG 
CTGCGCGGGG GTTTGGTACA AAACTCCAGC ATGCTGGCGA TGCTGGAGGA CACACCTATG 
GGAGCTCCGC AGGACCCGGC CGGCCAGTAT CAGCAGAACC AGCAGCTCTA CGACATGAGC 
AGCGGTACTC CGTTTCGCAA ACAGTTCAAA TACCTATCGG CCATCTCACC GCCAACACCG 
AGCTTCGGCA TCATGCCTTT AACTAGTCCG TGCACCGGCA ACGATGGATC CTTCATTGGC 
AACCACACGC CCGTTATGAA TATAAGCTAT TCGCCGATGC CACAGATGTT GGTCGAGGTC 
AACCAGGAGC CGAAGATGAT GGGCAAAAAG CTAAAGACAC ATGTGGGCGG ACTCATCAAT 
CGCAAAGAGG GCAGCCTTAA TAAACCCGCC GTCTTTACGC AAGCCGGAAA CATAACGCCC 
CGCACTCCGA ACAACAATAA TGTGGGCAAC AATGGCAATA TGAATGTGCC GCCCAATCCG 
AATGCTGCAG TGCGTCGCAG TTCGCGGCTG TTCAGCAACT CGTATTCGGT GAAGGAGAAC 
AATAAATCGC CCAACATAGC CAACAAGTTC GTGCAGCCAC GTTCGCCGCC CCGCAAAGCC 
AAGTCACGGA TGACCAAGAT CTGCCTAAAT AACGAACTGA TCGAGGATAA GTCCCACCAC 
CTCTCCGAGA AGCGCAAGGA GAAGGTGGAG ACTATTACTT CCTCGGGAGC GAATAACAAC 
AGCGGTGGAC GCAGTGCCGC CGAGGAGGCC AAAGTACTGC TGAACAATAG CCTAAACAAT 
GCCCAGACGA TGGCCCACCA GCTGATGGGA CTCAAGAAAC AATCGGCCGA TGGGCTGATG 
GCCCTGCTGC GGGGTTTAGC TGAAGCCTAT CAGCTGCTCT CGAACTTCCA GTGCAAGGCG 
GCCATAAAGC AGCTGGAGAC GACGATACCA AAGCACCACC TGAACTCCAG CTGGGTGCAG 
TCGCTCATCG GACTGGCCAG GTACGAGATG CGTGAGTATG AGGCGGCGGT GGCGATATTT 
GAGACCATCC ACAAGACAGA GCCATGTCGC TTGGACTACA TGGAGATATA CTCGTCATCC 
CTGTGGCATT TGCAGCGCGA GGTTGAGCTG TCAGCTCTCG CCCAGGATCT GATTAACCAG 
GACAAAACCA GCCCGGTTAC CTGGTGTGTG TCCGGCAATT GCTTTTCGCT GCAAAAGGAG 
CACGAAACCG CGATCAAGTT CTTCAAGCGG GCTGTCCAAG TGGATCCAGA CTTCGTCTAC 
AGCTACACAC TGTTGGGCCA CGAACTCGTA CTCACCGAAG AATTTGATAA GGCCATGGAC 
TACTTCCGTG CAGCAGTGGT TAGAGATCCG CGGCATTACA ATGCTTGGTA CGGCATCGGC 
ACCATCTACT CCAAGCAGGA GAAATACGAG CTGGCTGAGA TTCACTACGT GAAGGCGCTG 
AAAATCAATC CACAGAACTC GGTTATTCTC GTGCACATCG GCGCCATGCA ATTCTACATG 
AAGAAGAAGG ATCTGTCGCT GCAGACGCTA AACACGGCCG CCACGCTGGA CCCCAAGAAC 
CCGCTGACGC GATTCCATCG CGGCTCCATC TACTTCTCGC TGGGCAAGTA CCAGGAGGCG 
CTGCGCGAAC TGGAGGAGCT CAAGGAAGTC GTGCCCAAGG AGTCGGTGGT GTTCTATCTG 
ATCGGCAAAA TCCACAAGAC GCTCGGCAAC ATGGATCTGG CGCTGATGCA CTTCAGCTGG 
GCCACCGACC TAGATCCCAA AGGTGCCAAC AATCAAATCA AAGATGCCTT CGACTCAATG 
GCTCATCCCA GCTGCTGTCC GGCCAACACT ACGGCCCTGG ACGTTGATCT GGAGCCCACT 
TCGGAGCGTT CCGATGACTC GACGCAGGCG CAGCAGGACG GAAGCTACGA CAGCGACTAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648093.2
CDS	660..3362
Translation	MMIQEPVQAAIWHCLNYYDFKDAVFLSERLCSEVESDETIFLLATSYFRSNQVHQAYWLLKEKARRSPQCRFLQAKCAYELKKYAEAESALISTGFADAKNCDELQRDFGDLACFAYQLMAQICMRTERNKLAVSALRRALKLNPFMWHAFADLCLLGQDTDAAAIFQIHSTDVFNTCQGSSVNANAMVLFGAEQQGQQHQERQSLITNLSNVSNYILTTPVDQQQQQINQNQNQHHNSNMVTPINNNNNNNNLNSSISMLRGGLVQNSSMLAMLEDTPMGAPQDPAGQYQQNQQLYDMSSGTPFRKQFKYLSAISPPTPSFGIMPLTSPCTGNDGSFIGNHTPVMNISYSPMPQMLVEVNQEPKMMGKKLKTHVGGLINRKEGSLNKPAVFTQAGNITPRTPNNNNVGNNGNMNVPPNPNAAVRRSSRLFSNSYSVKENNKSPNIANKFVQPRSPPRKAKSRMTKICLNNELIEDKSHHLSEKRKEKVETITSSGANNNSGGRSAAEEAKVLLNNSLNNAQTMAHQLMGLKKQSADGLMALLRGLAEAYQLLSNFQCKAAIKQLETTIPKHHLNSSWVQSLIGLARYEMREYEAAVAIFETIHKTEPCRLDYMEIYSSSLWHLQREVELSALAQDLINQDKTSPVTWCVSGNCFSLQKEHETAIKFFKRAVQVDPDFVYSYTLLGHELVLTEEFDKAMDYFRAAVVRDPRHYNAWYGIGTIYSKQEKYELAEIHYVKALKINPQNSVILVHIGAMQFYMKKKDLSLQTLNTAATLDPKNPLTRFHRGSIYFSLGKYQEALRELEELKEVVPKESVVFYLIGKIHKTLGNMDLALMHFSWATDLDPKGANNQIKDAFDSMAHPSCCPANTTALDVDLEPTSERSDDSTQAQQDGSYDSDY

Target ORF information:

RefSeq Version	NM_139836.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cell division cycle 27, transcript variant A (Cdc27), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139836.3

ORF Insert Sequence:

ATGATGATTC AAGAGCCCGT GCAGGCCGCC ATATGGCATT GCCTGAACTA CTACGACTTC 
AAGGATGCCG TTTTCCTGTC GGAACGCCTG TGCTCAGAAG TGGAGAGCGA CGAGACGATA 
TTCCTGCTGG CCACCAGCTA CTTCCGCTCC AATCAGGTGC ACCAGGCATA TTGGCTGCTC 
AAGGAGAAGG CACGCCGCTC GCCGCAGTGC CGCTTTCTGC AGGCCAAATG TGCCTACGAG 
CTGAAAAAAT ACGCTGAGGC GGAGAGTGCC CTGATCTCCA CGGGATTCGC GGACGCCAAG 
AACTGCGATG AGCTGCAGCG GGACTTCGGG GATCTGGCCT GCTTCGCCTA TCAGCTAATG 
GCCCAGATCT GCATGAGGAC CGAGCGCAAT AAACTGGCCG TTAGTGCTTT AAGGCGCGCC 
CTCAAACTGA ACCCCTTCAT GTGGCACGCA TTCGCGGATC TGTGCCTGCT GGGCCAGGAT 
ACAGATGCGG CGGCCATATT CCAAATCCAC AGCACAGATG TGTTCAATAC CTGCCAGGGC 
AGCAGCGTAA ATGCCAATGC AATGGTGCTG TTCGGAGCCG AGCAGCAGGG TCAACAGCAT 
CAGGAGCGCC AGTCGCTGAT CACCAACCTA AGCAATGTCT CCAACTACAT TCTGACCACG 
CCCGTGGATC AGCAGCAACA ACAGATCAAC CAGAATCAGA ACCAGCACCA CAACAGCAAC 
ATGGTGACGC CCATCAACAA TAATAATAAT AACAACAATC TCAATAGTTC GATCAGCATG 
CTGCGCGGGG GTTTGGTACA AAACTCCAGC ATGCTGGCGA TGCTGGAGGA CACACCTATG 
GGAGCTCCGC AGGACCCGGC CGGCCAGTAT CAGCAGAACC AGCAGCTCTA CGACATGAGC 
AGCGGTACTC CGTTTCGCAA ACAGTTCAAA TACCTATCGG CCATCTCACC GCCAACACCG 
AGCTTCGGCA TCATGCCTTT AACTAGTCCG TGCACCGGCA ACGATGGATC CTTCATTGGC 
AACCACACGC CCGTTATGAA TATAAGCTAT TCGCCGATGC CACAGATGTT GGTCGAGGTC 
AACCAGGAGC CGAAGATGAT GGGCAAAAAG CTAAAGACAC ATGTGGGCGG ACTCATCAAT 
CGCAAAGAGG GCAGCCTTAA TAAACCCGCC GTCTTTACGC AAGCCGGAAA CATAACGCCC 
CGCACTCCGA ACAACAATAA TGTGGGCAAC AATGGCAATA TGAATGTGCC GCCCAATCCG 
AATGCTGCAG TGCGTCGCAG TTCGCGGCTG TTCAGCAACT CGTATTCGGT GAAGGAGAAC 
AATAAATCGC CCAACATAGC CAACAAGTTC GTGCAGCCAC GTTCGCCGCC CCGCAAAGCC 
AAGTCACGGA TGACCAAGAT CTGCCTAAAT AACGAACTGA TCGAGGATAA GTCCCACCAC 
CTCTCCGAGA AGCGCAAGGA GAAGGTGGAG ACTATTACTT CCTCGGGAGC GAATAACAAC 
AGCGGTGGAC GCAGTGCCGC CGAGGAGGCC AAAGTACTGC TGAACAATAG CCTAAACAAT 
GCCCAGACGA TGGCCCACCA GCTGATGGGA CTCAAGAAAC AATCGGCCGA TGGGCTGATG 
GCCCTGCTGC GGGGTTTAGC TGAAGCCTAT CAGCTGCTCT CGAACTTCCA GTGCAAGGCG 
GCCATAAAGC AGCTGGAGAC GACGATACCA AAGCACCACC TGAACTCCAG CTGGGTGCAG 
TCGCTCATCG GACTGGCCAG GTACGAGATG CGTGAGTATG AGGCGGCGGT GGCGATATTT 
GAGACCATCC ACAAGACAGA GCCATGTCGC TTGGACTACA TGGAGATATA CTCGTCATCC 
CTGTGGCATT TGCAGCGCGA GGTTGAGCTG TCAGCTCTCG CCCAGGATCT GATTAACCAG 
GACAAAACCA GCCCGGTTAC CTGGTGTGTG TCCGGCAATT GCTTTTCGCT GCAAAAGGAG 
CACGAAACCG CGATCAAGTT CTTCAAGCGG GCTGTCCAAG TGGATCCAGA CTTCGTCTAC 
AGCTACACAC TGTTGGGCCA CGAACTCGTA CTCACCGAAG AATTTGATAA GGCCATGGAC 
TACTTCCGTG CAGCAGTGGT TAGAGATCCG CGGCATTACA ATGCTTGGTA CGGCATCGGC 
ACCATCTACT CCAAGCAGGA GAAATACGAG CTGGCTGAGA TTCACTACGT GAAGGCGCTG 
AAAATCAATC CACAGAACTC GGTTATTCTC GTGCACATCG GCGCCATGCA ATTCTACATG 
AAGAAGAAGG ATCTGTCGCT GCAGACGCTA AACACGGCCG CCACGCTGGA CCCCAAGAAC 
CCGCTGACGC GATTCCATCG CGGCTCCATC TACTTCTCGC TGGGCAAGTA CCAGGAGGCG 
CTGCGCGAAC TGGAGGAGCT CAAGGAAGTC GTGCCCAAGG AGTCGGTGGT GTTCTATCTG 
ATCGGCAAAA TCCACAAGAC GCTCGGCAAC ATGGATCTGG CGCTGATGCA CTTCAGCTGG 
GCCACCGACC TAGATCCCAA AGGTGCCAAC AATCAAATCA AAGATGCCTT CGACTCAATG 
GCTCATCCCA GCTGCTGTCC GGCCAACACT ACGGCCCTGG ACGTTGATCT GGAGCCCACT 
TCGGAGCGTT CCGATGACTC GACGCAGGCG CAGCAGGACG GAAGCTACGA CAGCGACTAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Cdc27 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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