CG8219 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG8219
Entrez Gene ID	38722
Full Name	CG8219 gene product from transcript CG8219-RA
Synonyms	148274_at,Dmel\CG8219,EP(3)3072
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 65A8-65A8

mRNA and Protein(s)

mRNA	Protein	Name
NM_139781.1	NP_648038.1	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG8219 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG8219 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa17694	NM_139781.1 Latest version!	Drosophila melanogaster uncharacterized protein (CG8219), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG8219 ( NM_139781.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648038.1 Drosophila melanogaster uncharacterized protein (CG8219), mRNA.

Price & Availability↑

CloneID	OFa17694
Clone ID Related Accession (Same CDS sequence)	NM_139781.1
Accession Version	NM_139781.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCATGGA CACCACGGGA TGAAGGTCTG CAGCAGCTCC TACCGATACT CAAGGACTCA 
CAGTCTCCCG GTAAAGCCAC GCAGCTAGCC GTGCAAGAGA CGGAAAACGA GGCAACGCGC 
TCCATGAGCG GCCTAATCCT CAAGAACAAC ATTCGCATGC ACGACATCAC TCTGCAGCCG 
GAACACCTGG AGTATATCAA ACACCAGTGC CTGCAGGCAG TGGGCGACTC TTCACCCGAG 
ATCCGTGGCA CCGTGGGCAT TCTGATTACC ACCATTGCCA GCAATATCGG TCTGCACAAC 
TGGCCGCAGC TGCTTCCATC TCTCTGCGAA ATGCTCGACA ACCAGGACTA CAATGTGTGC 
GAAGGAGCAT TCAGTGCTCT GCAGAAAATT TGCGAGGACT CTGCCGGAAT TTTGGAAAAT 
ATGCCATTAA ATACAATGAT TCCAAAGTTC CTGGAGTACT TTAAGCACAG CAGTCCAAAG 
ATCCGCTCCC ACGCCATTGC CTGCATCAAC CAGTTCATCA TCAACAGATC ACAGGCCCTG 
ATGCTAAACA TAGACAGCCT CATTCAGAAC CTCTTAGACG TGCCCTCGGA TAATGACCCA 
GCGGTTCGCA TGAACGTCTG CCACGCATTG GTTGGTCTGG TGGGGGTGCG CATAGACCTT 
ATGATGCCGC ACATGTCGCA GATTATTGAG CTCATTTTGT TGCGCTCCCA GGACGCCGAT 
GAGAACGTGG CTCTGCAGGC CACCGAATTT TGGCTTTCAT TGGGAAAGCA GAGAAACTGC 
AGGGATATAC TTTCTCCCAT TCTATCCCAG TTAGTCCCAG TTCTTGTAAG CCGAACGCAA 
TACACGGAGA CGGACATTAT TCTTCTGAAG GGCGATGTAG GTGAAGATGA CAAGGAGCCT 
GATAGACAGC AGGATATCAG TCCACGTTTC CACATGTCCC GCGTACACGG TATAAGTAAT 
GAATTGGATG AAAACTCGGA CGACGATATG TGGGATTCGG CATTGAACTT GCGCAAGTGC 
AGCGCTTGTG CTCTCGACAT TATTTCGAAA ATTTTCGGCG ATGTCTGTCT GCCCCTTATG 
CTGCCCATCC TAAAGGAGGC GCTATTCCAC CAGGAATGGG TGATCAAAGA AAGTGGTGTG 
ATGGCCTTGG GAGCTATTGC GGAGGGCTGC ATGCAGGGCT TGATCCCACA CTTACCAGAG 
CTGATTCCCT ACCTAATTAC CTGTCTGTCT GACAAGAAGC CACTCGTACG CTCCATTACA 
TGCTGGACGT TCATGCGATT CCCCAAATGG GTGCTCAACC AGCCGCACGA CAAGTACTTG 
GAGCCTCTAA TCGAGGAACT GCTGAAGTGC ATCCTGGACT CCAATAAGCG CGTTCAGGAA 
GCTGCGTGCT CTGCCTTTGT CGCTCTGGAG GAGGAGGCCT GCACGCAACT GGTGCCCTAC 
CTGGAGAATA TGCTAAAGAC GTTCGTCTTA GCCTTCTCCA AGTACCACCA AAGGAATCTA 
CTGATAATGT ACGATGTCGT TGGTCTTTTA GCGGAGTCCG TCGGTCATCA TCTGAATAAA 
CCGCAATATA TTGACATCCT AATGCCTCCG CTAATGGATA AGTGGAACCT GGTAAAGGAC 
GACGACAAAG ATATAATCTA TTTGTTGGAG TGCCTATCGA GCATCGCCAC TGCGTTGCAG 
TCCTCCTTTC TGCCCTACTG CGACTCGGTG TATCGAAAGG GCATCTCCAT TATTGAGGAG 
ACTATCAACC AGGACATGAT GTGTAAACAA AGCCAATCAA CCGATTATCC TGACAAGGAG 
CGCATGGTTA TCGCCCTGGA TCTGGTGTCT GGCTTGATCG AGGGGCTGCA GAGCCTCATA 
GAGCCACTGG TGGCCAACAG CAAACTTTTG CAGCTGCTCT GCCAATGCAT GGGCGACGTT 
ATGCCCGAAG TTCGCCAATC ATCGTTTGCC CTGCTGGGTG ATTTGAGCGG GGCTTGTTTT 
TCCCAGGTAT ACCCCTACAC GGCCCACTTT ATTTTGATCT TGGTGCAAAA CCTAAACCCC 
AGCGTTGTTG GTGTGTGCAA CAATGCAGTT TGGGCCATAG GGCAAATCTG CGTTAAGTTG 
GGTGAGGAAA CCAAGCCGTA CGTACGCCTT CTGCTTAGCG AACTAATGAT CCTGATGAAC 
CGGCAGGACA TTCCAAAAAA TCTTTCGAGG AATGCGGCAA TAACACTTGG GCGCCTCGGA 
AATGCATGTC CAGATGAAGT GGCTCCCTAT TTGCCCGAAT TCCTACGCCA GTGGTGCTTG 
TTATTGCGTT ACGCATATGA CCACGACGAG AAGTATTTGT CTTTTATGGG AATGTGCCAT 
ATGATCAGGG AGAATCCAGG AGGCGTGGTG CCCGACTTCT TATTCTTTTG CGATGCCATT 
GCCTCGTGGG ACAATCCTCC ACAGGATCTG CGTCAGATGA TTCGAAGAAT TATCGAAGGC 
TTTAAGAACC AAACGGGTGC GGAGAATTGG CTAATTTTTT TGGGGAAATT TCCACAACCA 
CTGGCCTGCC GGCTAATCGA ACTGTACGAC ATCGAATACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648038.1
CDS	68..2629
Translation	MAWTPRDEGLQQLLPILKDSQSPGKATQLAVQETENEATRSMSGLILKNNIRMHDITLQPEHLEYIKHQCLQAVGDSSPEIRGTVGILITTIASNIGLHNWPQLLPSLCEMLDNQDYNVCEGAFSALQKICEDSAGILENMPLNTMIPKFLEYFKHSSPKIRSHAIACINQFIINRSQALMLNIDSLIQNLLDVPSDNDPAVRMNVCHALVGLVGVRIDLMMPHMSQIIELILLRSQDADENVALQATEFWLSLGKQRNCRDILSPILSQLVPVLVSRTQYTETDIILLKGDVGEDDKEPDRQQDISPRFHMSRVHGISNELDENSDDDMWDSALNLRKCSACALDIISKIFGDVCLPLMLPILKEALFHQEWVIKESGVMALGAIAEGCMQGLIPHLPELIPYLITCLSDKKPLVRSITCWTFMRFPKWVLNQPHDKYLEPLIEELLKCILDSNKRVQEAACSAFVALEEEACTQLVPYLENMLKTFVLAFSKYHQRNLLIMYDVVGLLAESVGHHLNKPQYIDILMPPLMDKWNLVKDDDKDIIYLLECLSSIATALQSSFLPYCDSVYRKGISIIEETINQDMMCKQSQSTDYPDKERMVIALDLVSGLIEGLQSLIEPLVANSKLLQLLCQCMGDVMPEVRQSSFALLGDLSGACFSQVYPYTAHFILILVQNLNPSVVGVCNNAVWAIGQICVKLGEETKPYVRLLLSELMILMNRQDIPKNLSRNAAITLGRLGNACPDEVAPYLPEFLRQWCLLLRYAYDHDEKYLSFMGMCHMIRENPGGVVPDFLFFCDAIASWDNPPQDLRQMIRRIIEGFKNQTGAENWLIFLGKFPQPLACRLIELYDIEY

Target ORF information:

RefSeq Version	NM_139781.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein (CG8219), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139781.1

ORF Insert Sequence:

ATGGCATGGA CACCACGGGA TGAAGGTCTG CAGCAGCTCC TACCGATACT CAAGGACTCA 
CAGTCTCCCG GTAAAGCCAC GCAGCTAGCC GTGCAAGAGA CGGAAAACGA GGCAACGCGC 
TCCATGAGCG GCCTAATCCT CAAGAACAAC ATTCGCATGC ACGACATCAC TCTGCAGCCG 
GAACACCTGG AGTATATCAA ACACCAGTGC CTGCAGGCAG TGGGCGACTC TTCACCCGAG 
ATCCGTGGCA CCGTGGGCAT TCTGATTACC ACCATTGCCA GCAATATCGG TCTGCACAAC 
TGGCCGCAGC TGCTTCCATC TCTCTGCGAA ATGCTCGACA ACCAGGACTA CAATGTGTGC 
GAAGGAGCAT TCAGTGCTCT GCAGAAAATT TGCGAGGACT CTGCCGGAAT TTTGGAAAAT 
ATGCCATTAA ATACAATGAT TCCAAAGTTC CTGGAGTACT TTAAGCACAG CAGTCCAAAG 
ATCCGCTCCC ACGCCATTGC CTGCATCAAC CAGTTCATCA TCAACAGATC ACAGGCCCTG 
ATGCTAAACA TAGACAGCCT CATTCAGAAC CTCTTAGACG TGCCCTCGGA TAATGACCCA 
GCGGTTCGCA TGAACGTCTG CCACGCATTG GTTGGTCTGG TGGGGGTGCG CATAGACCTT 
ATGATGCCGC ACATGTCGCA GATTATTGAG CTCATTTTGT TGCGCTCCCA GGACGCCGAT 
GAGAACGTGG CTCTGCAGGC CACCGAATTT TGGCTTTCAT TGGGAAAGCA GAGAAACTGC 
AGGGATATAC TTTCTCCCAT TCTATCCCAG TTAGTCCCAG TTCTTGTAAG CCGAACGCAA 
TACACGGAGA CGGACATTAT TCTTCTGAAG GGCGATGTAG GTGAAGATGA CAAGGAGCCT 
GATAGACAGC AGGATATCAG TCCACGTTTC CACATGTCCC GCGTACACGG TATAAGTAAT 
GAATTGGATG AAAACTCGGA CGACGATATG TGGGATTCGG CATTGAACTT GCGCAAGTGC 
AGCGCTTGTG CTCTCGACAT TATTTCGAAA ATTTTCGGCG ATGTCTGTCT GCCCCTTATG 
CTGCCCATCC TAAAGGAGGC GCTATTCCAC CAGGAATGGG TGATCAAAGA AAGTGGTGTG 
ATGGCCTTGG GAGCTATTGC GGAGGGCTGC ATGCAGGGCT TGATCCCACA CTTACCAGAG 
CTGATTCCCT ACCTAATTAC CTGTCTGTCT GACAAGAAGC CACTCGTACG CTCCATTACA 
TGCTGGACGT TCATGCGATT CCCCAAATGG GTGCTCAACC AGCCGCACGA CAAGTACTTG 
GAGCCTCTAA TCGAGGAACT GCTGAAGTGC ATCCTGGACT CCAATAAGCG CGTTCAGGAA 
GCTGCGTGCT CTGCCTTTGT CGCTCTGGAG GAGGAGGCCT GCACGCAACT GGTGCCCTAC 
CTGGAGAATA TGCTAAAGAC GTTCGTCTTA GCCTTCTCCA AGTACCACCA AAGGAATCTA 
CTGATAATGT ACGATGTCGT TGGTCTTTTA GCGGAGTCCG TCGGTCATCA TCTGAATAAA 
CCGCAATATA TTGACATCCT AATGCCTCCG CTAATGGATA AGTGGAACCT GGTAAAGGAC 
GACGACAAAG ATATAATCTA TTTGTTGGAG TGCCTATCGA GCATCGCCAC TGCGTTGCAG 
TCCTCCTTTC TGCCCTACTG CGACTCGGTG TATCGAAAGG GCATCTCCAT TATTGAGGAG 
ACTATCAACC AGGACATGAT GTGTAAACAA AGCCAATCAA CCGATTATCC TGACAAGGAG 
CGCATGGTTA TCGCCCTGGA TCTGGTGTCT GGCTTGATCG AGGGGCTGCA GAGCCTCATA 
GAGCCACTGG TGGCCAACAG CAAACTTTTG CAGCTGCTCT GCCAATGCAT GGGCGACGTT 
ATGCCCGAAG TTCGCCAATC ATCGTTTGCC CTGCTGGGTG ATTTGAGCGG GGCTTGTTTT 
TCCCAGGTAT ACCCCTACAC GGCCCACTTT ATTTTGATCT TGGTGCAAAA CCTAAACCCC 
AGCGTTGTTG GTGTGTGCAA CAATGCAGTT TGGGCCATAG GGCAAATCTG CGTTAAGTTG 
GGTGAGGAAA CCAAGCCGTA CGTACGCCTT CTGCTTAGCG AACTAATGAT CCTGATGAAC 
CGGCAGGACA TTCCAAAAAA TCTTTCGAGG AATGCGGCAA TAACACTTGG GCGCCTCGGA 
AATGCATGTC CAGATGAAGT GGCTCCCTAT TTGCCCGAAT TCCTACGCCA GTGGTGCTTG 
TTATTGCGTT ACGCATATGA CCACGACGAG AAGTATTTGT CTTTTATGGG AATGTGCCAT 
ATGATCAGGG AGAATCCAGG AGGCGTGGTG CCCGACTTCT TATTCTTTTG CGATGCCATT 
GCCTCGTGGG ACAATCCTCC ACAGGATCTG CGTCAGATGA TTCGAAGAAT TATCGAAGGC 
TTTAAGAACC AAACGGGTGC GGAGAATTGG CTAATTTTTT TGGGGAAATT TCCACAACCA 
CTGGCCTGCC GGCTAATCGA ACTGTACGAC ATCGAATACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG8219 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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