Bre1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Bre1
Entrez Gene ID	38652
Full Name	CG10542 gene product from transcript CG10542-RB
Synonyms	BRE1,Bre-1,CG10542,Dmel\CG10542,EC3-8,bre-1,bre1,dBRE1,dBre1,l(3)01640
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 64E8-64E8

mRNA and Protein(s)

mRNA	Protein	Name
NM_001300021.1	NP_001286950.1	Bre1, isoform B
NM_139732.3	NP_647989.2	Bre1, isoform A

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Rnf40	NP_758485.2
Homo sapiens (human)	RNF40	NP_055586.1
	RNF40	XP_001102375.2
Pan troglodytes (chimpanzee)	RNF40	XP_003315121.1
Rattus norvegicus (Norway rat)	Rnf40	NP_703201.1
Xenopus tropicalis (tropical clawed frog)	rnf40	XP_004918162.1
Drosophila melanogaster (fruit fly)	Bre1	NP_647989.2
Canis lupus familiaris (dog)	RNF40	XP_005621328.1
Bos taurus (cattle)	RNF40	NP_001193381.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007335	XP_308493.4
Caenorhabditis elegans (roundworm)	rfp-1	NP_001022700.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

dBre1/dSet1-dependent pathway for histone H3K4 trimethylation has essential roles in controlling germline stem cell maintenance and germ cell differentiation in the Drosophila ovary.
Title: dBre1/dSet1-dependent pathway for histone H3K4 trimethylation has essential roles in controlling germline stem cell maintenance and germ cell differentiation in the Drosophila ovary.

identify Bre1 as a nuclear component required cell autonomously for the expression of Notch target genes in development
Title: Bre1 is required for Notch signaling and histone modification.

The following Bre1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Bre1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa17629	NM_139732.3 Latest version!	Drosophila melanogaster Bre1, transcript variant A (Bre1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa17629	NM_001300021.1 Latest version!	Drosophila melanogaster Bre1, transcript variant B (Bre1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Bre1 ( NM_001300021.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286950.1 Drosophila melanogaster Bre1, transcript variant B (Bre1), mRNA.

Price & Availability↑

CloneID	OFa17629
Clone ID Related Accession (Same CDS sequence)	NM_001300021.1 , NM_139732.3
Accession Version	NM_001300021.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Bre1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGAAAC GGAGCGCCGA CGACGCCACC GGCAGCAGCT GCTTGGTGGC CGCCGCAGCC 
GCCGGCCAGC CGCCCATCAA GAAGGTGCAC TTCGAGCCCC ATCTGATTGG GCCGGTGTCC 
ACGCTGGAGG AGATGGACAT CAAGGTGCTG GAATTCCAGA ACAAGAAGCT AGCACAGCGC 
ATCGAACAGC GGATGCGAAC GGAGGCGGAG CTGCGACATC GAATCGAGCA GCTGGAAAAG 
CGGCAAACGC AGGACGACGC CGTTCTAAAC GTGGTCAATC GGTATTGGAA TCAGCTGAAC 
GAGGACATAC GAGTCCTGCT GCAGCGCTTC GATGCCGAGA CGGCCGACGA ACTGGAGAAC 
CGGAATGAGA ACGAGGTGAC CACCTCGTTT CTCGCCCAAC TGTCCACCTG GGACAAGGAG 
GAGCTGGACG AGAAGCTGGC CAACCGCGTC CAAGTGTCCA AAAGGGCGGT CGCCAAGATC 
GTACAGGTTA TTGATAGGTT AATGCAACGC AATGAAAAGA TAACTCATGT CTTGAAAGGT 
GATAGTCTGG CTTCAGCTGG CTCCGGTTCA GGAGCGGGAG CTGGCGGCGA GGAGGAGCAG 
CAGCAGGCCA GCGGCGATGC GGAGACCACA ACGTCATCGG CCGGTGTCCA TGCGCTAGAG 
GAAACCCTGA AGCAAACGCA CATCGAGATC ATGAGCGAAA ATCACAAGCT GCAGAATCTG 
AATACCTCGC TGCACGAGAA GTTCCACACC ATGTCGCTGA AGATGAAGGA GTACCAGGAC 
GCTCATACCG CCAAGGAGAC GGAGAACGCC GAACTGAAGA ACCAGATAGA CGAACTGCAA 
TATGACCTAG AGAAGATCCA TTGCCGGAAT GATAAGCTGG AGAACCACTT GGCCGAGGCT 
ATTGAAAAGC TGAAGGCCTA CCACCAGATT TACGGTGATC CCAATAAGAG CACTAATAGT 
GCCAAGACAC CAACTACCAC AGGCAGCGGC GGCGCCACCA CCAGCGTTAA TAGCCAGCTA 
TTGGAGGAGC TGCAAAAGGA GCTGGAGGAG TACCGCGAAC TGGCCAACAA CCGACTGCAG 
GAGCTGGATA AGCTTCACGC CACGCATCGC GAAACCCTGA AGGAGGTGGA GAAGCTTAAA 
ATGGATATAC GACAGTTGCC GGAATCGGTA ATTGTGGAAA CCACGGAGTA CAAGTGCCTG 
CAGAGCCAGT TTTCCGTGCT CTACAACGAG TCCATGCAGA TAAAGACCAT GCTGGACGAG 
ACCCGAAACC AGCTTCAAAC GAGCAAGAAC CAGCATCTGC GACAAATCGA AGTGATGGAG 
AGCGAGGAAC TTATTGCCCA GAAGAAGGTG CGCAGCGAGA TGATCCAGAT GGAGGACGTT 
TTAGCCCTCA TCCGAAAGGA GTACGAAACG CTACGCATTG AGTTCGAGCA GAACATGGCA 
GCCAACGAGC AGACGGCACC CATCAATCGC GAGATGCGCC ACCTGATCAC CTCTCTACAA 
AACCACAATG GCCAACTGAA GGGTGAGGTG CAGCGCTACA AGCGAAAGTA CAAGGACACG 
TCCACGGACA ACTTGAAGCT GCGTCAGGAG CTGGCCGATG CACTAGCTAC ACTCGAAGGC 
AACAAGTTGC AGGCGGCGAC TGGTGCGGCT GGCGAAGAAA TCAAGCAGGA GAACTCAACA 
GGTGTGAAGG AGGAGAACTC CAACAACGTT TCCGCCAGTG GACAAACAAA CCAAACAAAT 
TCCGGCAACG ATACCAACGT AGCCATCAAG GAGGAGAACC ATATCTCGGC TGAAGATGAA 
GCTGATGACG AGGCCAGTGG TAAGGACGTA AAAGACGGCA TCAAGCAGGA GAAGCTCAGT 
TCTGGAGACG CGGCGGCAGC TGAGAAGAAG GATTCCCCTG GACCCGGAAA CTCAACAAGT 
TCTGCGACCA ATTCTGTTCC CGTCAAAAAC GAAAAGGATT CCAAGGATGG TGTCAAGGGC 
AAGGATGTGA AGGCAGTGGA AAGTGAAACG GTGCGCGATC TGAAGGCGCA ACTAAAAAAA 
GCACTGAACG ACCAGAAAGA AATGAAACTC CTGCTGGATA TGTACAAAGG CGTATCGAAG 
GATCAGCGCG ACAAGGTCCA GTTGATGGCC ACCGAAAAGA AACTGCGCTC CGAGATCGAG 
GAGCTCCGCC AGCAGCTGAA GAAGCTGCAG GAAAGCAAGC GCGAGGAACG CAAGAAGCTG 
GCCGACGAGG AGGCGTTACG TAAGATTAAG CAATTGGAGG AGCAGAAGTA CGAGCTGCAA 
AAACAGATGG CCAACCACAA GCCAACGGAT AATTCTTGGG GCAGTGGAGC CCCCGGCACA 
GCGAACTACA CGCGACCGTT TGTTGGTTCC CACGAAGAGG AGGCACTGCT GAACGAGATG 
GAGGTTACCG GCCAGGCGTT CGAGGACATG CAGGAGCAGA ACTCCCGGCT CATACAACAG 
CTGCGTGAGA AGGACGATGC GAACTTTAAG CTAATGTCGG AGCGCATCAA GGCCAACCAG 
CTGCACAAAC TTCTGAGAGA GGAAAAGACG GTGCTGGAGG ATCAGATGGC AACGGCGACC 
ACGCAAATCG AAGCCATGCA CATTGTGCTG CGAAAGCTGG AGGAGAAGGA ACGCAGCCTG 
CAGGCCACCG TCGCGTCGAT TGAGAAAGAG TTGATGCTGC GGCAGCAAGC AATGGAGATG 
CACAAACGCA AGGCGATTGA ATCGGCGCAA TCGGCAGCGG ACCTCAAGCT GCATCTGGAA 
AAGTATCACG CGCAAATGAA GGAGGCGCAG CAAGTGGTGG CGGAGAAGAC CAGTTCGCTG 
GAGGCGGAGG CCTACAAGAC CAAGCGGCTG CAGGAGGAGC TGGCGCAGTT TAAACGCAAG 
GCGGAGCGCA TGAAGAAGAT GGAAATGTCG GGCACCACCA TCGACGAGGT GATGATTGAG 
GAGATCCGCG AATACAAAGA AACGCTCACG TGTCCCTCGT GCAAGGTGAA GCGGAAGGAT 
GCTGTCCTGT CAAAGTGTTT CCACGTCTTC TGCTACGACT GCCTGCGCAC TAGATATGAA 
ACGCGGCAGC GGAAGTGTCC CAAATGCAAC TGCGCCTTCG GCGCCAACGA TTATCATAGG 
CTATATCTGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286950.1
CDS	145..3279
Translation	MSKRSADDATGSSCLVAAAAAGQPPIKKVHFEPHLIGPVSTLEEMDIKVLEFQNKKLAQRIEQRMRTEAELRHRIEQLEKRQTQDDAVLNVVNRYWNQLNEDIRVLLQRFDAETADELENRNENEVTTSFLAQLSTWDKEELDEKLANRVQVSKRAVAKIVQVIDRLMQRNEKITHVLKGDSLASAGSGSGAGAGGEEEQQQASGDAETTTSSAGVHALEETLKQTHIEIMSENHKLQNLNTSLHEKFHTMSLKMKEYQDAHTAKETENAELKNQIDELQYDLEKIHCRNDKLENHLAEAIEKLKAYHQIYGDPNKSTNSAKTPTTTGSGGATTSVNSQLLEELQKELEEYRELANNRLQELDKLHATHRETLKEVEKLKMDIRQLPESVIVETTEYKCLQSQFSVLYNESMQIKTMLDETRNQLQTSKNQHLRQIEVMESEELIAQKKVRSEMIQMEDVLALIRKEYETLRIEFEQNMAANEQTAPINREMRHLITSLQNHNGQLKGEVQRYKRKYKDTSTDNLKLRQELADALATLEGNKLQAATGAAGEEIKQENSTGVKEENSNNVSASGQTNQTNSGNDTNVAIKEENHISAEDEADDEASGKDVKDGIKQEKLSSGDAAAAEKKDSPGPGNSTSSATNSVPVKNEKDSKDGVKGKDVKAVESETVRDLKAQLKKALNDQKEMKLLLDMYKGVSKDQRDKVQLMATEKKLRSEIEELRQQLKKLQESKREERKKLADEEALRKIKQLEEQKYELQKQMANHKPTDNSWGSGAPGTANYTRPFVGSHEEEALLNEMEVTGQAFEDMQEQNSRLIQQLREKDDANFKLMSERIKANQLHKLLREEKTVLEDQMATATTQIEAMHIVLRKLEEKERSLQATVASIEKELMLRQQAMEMHKRKAIESAQSAADLKLHLEKYHAQMKEAQQVVAEKTSSLEAEAYKTKRLQEELAQFKRKAERMKKMEMSGTTIDEVMIEEIREYKETLTCPSCKVKRKDAVLSKCFHVFCYDCLRTRYETRQRKCPKCNCAFGANDYHRLYLQ

Target ORF information:

RefSeq Version	NM_001300021.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Bre1, transcript variant B (Bre1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300021.1

ORF Insert Sequence:

ATGTCGAAAC GGAGCGCCGA CGACGCCACC GGCAGCAGCT GCTTGGTGGC CGCCGCAGCC 
GCCGGCCAGC CGCCCATCAA GAAGGTGCAC TTCGAGCCCC ATCTGATTGG GCCGGTGTCC 
ACGCTGGAGG AGATGGACAT CAAGGTGCTG GAATTCCAGA ACAAGAAGCT AGCACAGCGC 
ATCGAACAGC GGATGCGAAC GGAGGCGGAG CTGCGACATC GAATCGAGCA GCTGGAAAAG 
CGGCAAACGC AGGACGACGC CGTTCTAAAC GTGGTCAATC GGTATTGGAA TCAGCTGAAC 
GAGGACATAC GAGTCCTGCT GCAGCGCTTC GATGCCGAGA CGGCCGACGA ACTGGAGAAC 
CGGAATGAGA ACGAGGTGAC CACCTCGTTT CTCGCCCAAC TGTCCACCTG GGACAAGGAG 
GAGCTGGACG AGAAGCTGGC CAACCGCGTC CAAGTGTCCA AAAGGGCGGT CGCCAAGATC 
GTACAGGTTA TTGATAGGTT AATGCAACGC AATGAAAAGA TAACTCATGT CTTGAAAGGT 
GATAGTCTGG CTTCAGCTGG CTCCGGTTCA GGAGCGGGAG CTGGCGGCGA GGAGGAGCAG 
CAGCAGGCCA GCGGCGATGC GGAGACCACA ACGTCATCGG CCGGTGTCCA TGCGCTAGAG 
GAAACCCTGA AGCAAACGCA CATCGAGATC ATGAGCGAAA ATCACAAGCT GCAGAATCTG 
AATACCTCGC TGCACGAGAA GTTCCACACC ATGTCGCTGA AGATGAAGGA GTACCAGGAC 
GCTCATACCG CCAAGGAGAC GGAGAACGCC GAACTGAAGA ACCAGATAGA CGAACTGCAA 
TATGACCTAG AGAAGATCCA TTGCCGGAAT GATAAGCTGG AGAACCACTT GGCCGAGGCT 
ATTGAAAAGC TGAAGGCCTA CCACCAGATT TACGGTGATC CCAATAAGAG CACTAATAGT 
GCCAAGACAC CAACTACCAC AGGCAGCGGC GGCGCCACCA CCAGCGTTAA TAGCCAGCTA 
TTGGAGGAGC TGCAAAAGGA GCTGGAGGAG TACCGCGAAC TGGCCAACAA CCGACTGCAG 
GAGCTGGATA AGCTTCACGC CACGCATCGC GAAACCCTGA AGGAGGTGGA GAAGCTTAAA 
ATGGATATAC GACAGTTGCC GGAATCGGTA ATTGTGGAAA CCACGGAGTA CAAGTGCCTG 
CAGAGCCAGT TTTCCGTGCT CTACAACGAG TCCATGCAGA TAAAGACCAT GCTGGACGAG 
ACCCGAAACC AGCTTCAAAC GAGCAAGAAC CAGCATCTGC GACAAATCGA AGTGATGGAG 
AGCGAGGAAC TTATTGCCCA GAAGAAGGTG CGCAGCGAGA TGATCCAGAT GGAGGACGTT 
TTAGCCCTCA TCCGAAAGGA GTACGAAACG CTACGCATTG AGTTCGAGCA GAACATGGCA 
GCCAACGAGC AGACGGCACC CATCAATCGC GAGATGCGCC ACCTGATCAC CTCTCTACAA 
AACCACAATG GCCAACTGAA GGGTGAGGTG CAGCGCTACA AGCGAAAGTA CAAGGACACG 
TCCACGGACA ACTTGAAGCT GCGTCAGGAG CTGGCCGATG CACTAGCTAC ACTCGAAGGC 
AACAAGTTGC AGGCGGCGAC TGGTGCGGCT GGCGAAGAAA TCAAGCAGGA GAACTCAACA 
GGTGTGAAGG AGGAGAACTC CAACAACGTT TCCGCCAGTG GACAAACAAA CCAAACAAAT 
TCCGGCAACG ATACCAACGT AGCCATCAAG GAGGAGAACC ATATCTCGGC TGAAGATGAA 
GCTGATGACG AGGCCAGTGG TAAGGACGTA AAAGACGGCA TCAAGCAGGA GAAGCTCAGT 
TCTGGAGACG CGGCGGCAGC TGAGAAGAAG GATTCCCCTG GACCCGGAAA CTCAACAAGT 
TCTGCGACCA ATTCTGTTCC CGTCAAAAAC GAAAAGGATT CCAAGGATGG TGTCAAGGGC 
AAGGATGTGA AGGCAGTGGA AAGTGAAACG GTGCGCGATC TGAAGGCGCA ACTAAAAAAA 
GCACTGAACG ACCAGAAAGA AATGAAACTC CTGCTGGATA TGTACAAAGG CGTATCGAAG 
GATCAGCGCG ACAAGGTCCA GTTGATGGCC ACCGAAAAGA AACTGCGCTC CGAGATCGAG 
GAGCTCCGCC AGCAGCTGAA GAAGCTGCAG GAAAGCAAGC GCGAGGAACG CAAGAAGCTG 
GCCGACGAGG AGGCGTTACG TAAGATTAAG CAATTGGAGG AGCAGAAGTA CGAGCTGCAA 
AAACAGATGG CCAACCACAA GCCAACGGAT AATTCTTGGG GCAGTGGAGC CCCCGGCACA 
GCGAACTACA CGCGACCGTT TGTTGGTTCC CACGAAGAGG AGGCACTGCT GAACGAGATG 
GAGGTTACCG GCCAGGCGTT CGAGGACATG CAGGAGCAGA ACTCCCGGCT CATACAACAG 
CTGCGTGAGA AGGACGATGC GAACTTTAAG CTAATGTCGG AGCGCATCAA GGCCAACCAG 
CTGCACAAAC TTCTGAGAGA GGAAAAGACG GTGCTGGAGG ATCAGATGGC AACGGCGACC 
ACGCAAATCG AAGCCATGCA CATTGTGCTG CGAAAGCTGG AGGAGAAGGA ACGCAGCCTG 
CAGGCCACCG TCGCGTCGAT TGAGAAAGAG TTGATGCTGC GGCAGCAAGC AATGGAGATG 
CACAAACGCA AGGCGATTGA ATCGGCGCAA TCGGCAGCGG ACCTCAAGCT GCATCTGGAA 
AAGTATCACG CGCAAATGAA GGAGGCGCAG CAAGTGGTGG CGGAGAAGAC CAGTTCGCTG 
GAGGCGGAGG CCTACAAGAC CAAGCGGCTG CAGGAGGAGC TGGCGCAGTT TAAACGCAAG 
GCGGAGCGCA TGAAGAAGAT GGAAATGTCG GGCACCACCA TCGACGAGGT GATGATTGAG 
GAGATCCGCG AATACAAAGA AACGCTCACG TGTCCCTCGT GCAAGGTGAA GCGGAAGGAT 
GCTGTCCTGT CAAAGTGTTT CCACGTCTTC TGCTACGACT GCCTGCGCAC TAGATATGAA 
ACGCGGCAGC GGAAGTGTCC CAAATGCAAC TGCGCCTTCG GCGCCAACGA TTATCATAGG 
CTATATCTGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bre1 ( NM_139732.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_647989.2 Drosophila melanogaster Bre1, transcript variant A (Bre1), mRNA.

Price & Availability↑

CloneID	OFa17629
Clone ID Related Accession (Same CDS sequence)	NM_001300021.1 , NM_139732.3
Accession Version	NM_139732.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Bre1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_139732.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGAAAC GGAGCGCCGA CGACGCCACC GGCAGCAGCT GCTTGGTGGC CGCCGCAGCC 
GCCGGCCAGC CGCCCATCAA GAAGGTGCAC TTCGAGCCCC ATCTGATTGG GCCGGTGTCC 
ACGCTGGAGG AGATGGACAT CAAGGTGCTG GAATTCCAGA ACAAGAAGCT AGCACAGCGC 
ATCGAACAGC GGATGCGAAC GGAGGCGGAG CTGCGACATC GAATCGAGCA GCTGGAAAAG 
CGGCAAACGC AGGACGACGC CGTTCTAAAC GTGGTCAATC GGTATTGGAA TCAGCTGAAC 
GAGGACATAC GAGTCCTGCT GCAGCGCTTC GATGCCGAGA CGGCCGACGA ACTGGAGAAC 
CGGAATGAGA ACGAGGTGAC CACCTCGTTT CTCGCCCAAC TGTCCACCTG GGACAAGGAG 
GAGCTGGACG AGAAGCTGGC CAACCGCGTC CAAGTGTCCA AAAGGGCGGT CGCCAAGATC 
GTACAGGTTA TTGATAGGTT AATGCAACGC AATGAAAAGA TAACTCATGT CTTGAAAGGT 
GATAGTCTGG CTTCAGCTGG CTCCGGTTCA GGAGCGGGAG CTGGCGGCGA GGAGGAGCAG 
CAGCAGGCCA GCGGCGATGC GGAGACCACA ACGTCATCGG CCGGTGTCCA TGCGCTAGAG 
GAAACCCTGA AGCAAACGCA CATCGAGATC ATGAGCGAAA ATCACAAGCT GCAGAATCTG 
AATACCTCGC TGCACGAGAA GTTCCACACC ATGTCGCTGA AGATGAAGGA GTACCAGGAC 
GCTCATACCG CCAAGGAGAC GGAGAACGCC GAACTGAAGA ACCAGATAGA CGAACTGCAA 
TATGACCTAG AGAAGATCCA TTGCCGGAAT GATAAGCTGG AGAACCACTT GGCCGAGGCT 
ATTGAAAAGC TGAAGGCCTA CCACCAGATT TACGGTGATC CCAATAAGAG CACTAATAGT 
GCCAAGACAC CAACTACCAC AGGCAGCGGC GGCGCCACCA CCAGCGTTAA TAGCCAGCTA 
TTGGAGGAGC TGCAAAAGGA GCTGGAGGAG TACCGCGAAC TGGCCAACAA CCGACTGCAG 
GAGCTGGATA AGCTTCACGC CACGCATCGC GAAACCCTGA AGGAGGTGGA GAAGCTTAAA 
ATGGATATAC GACAGTTGCC GGAATCGGTA ATTGTGGAAA CCACGGAGTA CAAGTGCCTG 
CAGAGCCAGT TTTCCGTGCT CTACAACGAG TCCATGCAGA TAAAGACCAT GCTGGACGAG 
ACCCGAAACC AGCTTCAAAC GAGCAAGAAC CAGCATCTGC GACAAATCGA AGTGATGGAG 
AGCGAGGAAC TTATTGCCCA GAAGAAGGTG CGCAGCGAGA TGATCCAGAT GGAGGACGTT 
TTAGCCCTCA TCCGAAAGGA GTACGAAACG CTACGCATTG AGTTCGAGCA GAACATGGCA 
GCCAACGAGC AGACGGCACC CATCAATCGC GAGATGCGCC ACCTGATCAC CTCTCTACAA 
AACCACAATG GCCAACTGAA GGGTGAGGTG CAGCGCTACA AGCGAAAGTA CAAGGACACG 
TCCACGGACA ACTTGAAGCT GCGTCAGGAG CTGGCCGATG CACTAGCTAC ACTCGAAGGC 
AACAAGTTGC AGGCGGCGAC TGGTGCGGCT GGCGAAGAAA TCAAGCAGGA GAACTCAACA 
GGTGTGAAGG AGGAGAACTC CAACAACGTT TCCGCCAGTG GACAAACAAA CCAAACAAAT 
TCCGGCAACG ATACCAACGT AGCCATCAAG GAGGAGAACC ATATCTCGGC TGAAGATGAA 
GCTGATGACG AGGCCAGTGG TAAGGACGTA AAAGACGGCA TCAAGCAGGA GAAGCTCAGT 
TCTGGAGACG CGGCGGCAGC TGAGAAGAAG GATTCCCCTG GACCCGGAAA CTCAACAAGT 
TCTGCGACCA ATTCTGTTCC CGTCAAAAAC GAAAAGGATT CCAAGGATGG TGTCAAGGGC 
AAGGATGTGA AGGCAGTGGA AAGTGAAACG GTGCGCGATC TGAAGGCGCA ACTAAAAAAA 
GCACTGAACG ACCAGAAAGA AATGAAACTC CTGCTGGATA TGTACAAAGG CGTATCGAAG 
GATCAGCGCG ACAAGGTCCA GTTGATGGCC ACCGAAAAGA AACTGCGCTC CGAGATCGAG 
GAGCTCCGCC AGCAGCTGAA GAAGCTGCAG GAAAGCAAGC GCGAGGAACG CAAGAAGCTG 
GCCGACGAGG AGGCGTTACG TAAGATTAAG CAATTGGAGG AGCAGAAGTA CGAGCTGCAA 
AAACAGATGG CCAACCACAA GCCAACGGAT AATTCTTGGG GCAGTGGAGC CCCCGGCACA 
GCGAACTACA CGCGACCGTT TGTTGGTTCC CACGAAGAGG AGGCACTGCT GAACGAGATG 
GAGGTTACCG GCCAGGCGTT CGAGGACATG CAGGAGCAGA ACTCCCGGCT CATACAACAG 
CTGCGTGAGA AGGACGATGC GAACTTTAAG CTAATGTCGG AGCGCATCAA GGCCAACCAG 
CTGCACAAAC TTCTGAGAGA GGAAAAGACG GTGCTGGAGG ATCAGATGGC AACGGCGACC 
ACGCAAATCG AAGCCATGCA CATTGTGCTG CGAAAGCTGG AGGAGAAGGA ACGCAGCCTG 
CAGGCCACCG TCGCGTCGAT TGAGAAAGAG TTGATGCTGC GGCAGCAAGC AATGGAGATG 
CACAAACGCA AGGCGATTGA ATCGGCGCAA TCGGCAGCGG ACCTCAAGCT GCATCTGGAA 
AAGTATCACG CGCAAATGAA GGAGGCGCAG CAAGTGGTGG CGGAGAAGAC CAGTTCGCTG 
GAGGCGGAGG CCTACAAGAC CAAGCGGCTG CAGGAGGAGC TGGCGCAGTT TAAACGCAAG 
GCGGAGCGCA TGAAGAAGAT GGAAATGTCG GGCACCACCA TCGACGAGGT GATGATTGAG 
GAGATCCGCG AATACAAAGA AACGCTCACG TGTCCCTCGT GCAAGGTGAA GCGGAAGGAT 
GCTGTCCTGT CAAAGTGTTT CCACGTCTTC TGCTACGACT GCCTGCGCAC TAGATATGAA 
ACGCGGCAGC GGAAGTGTCC CAAATGCAAC TGCGCCTTCG GCGCCAACGA TTATCATAGG 
CTATATCTGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_647989.2
CDS	145..3279
Translation	MSKRSADDATGSSCLVAAAAAGQPPIKKVHFEPHLIGPVSTLEEMDIKVLEFQNKKLAQRIEQRMRTEAELRHRIEQLEKRQTQDDAVLNVVNRYWNQLNEDIRVLLQRFDAETADELENRNENEVTTSFLAQLSTWDKEELDEKLANRVQVSKRAVAKIVQVIDRLMQRNEKITHVLKGDSLASAGSGSGAGAGGEEEQQQASGDAETTTSSAGVHALEETLKQTHIEIMSENHKLQNLNTSLHEKFHTMSLKMKEYQDAHTAKETENAELKNQIDELQYDLEKIHCRNDKLENHLAEAIEKLKAYHQIYGDPNKSTNSAKTPTTTGSGGATTSVNSQLLEELQKELEEYRELANNRLQELDKLHATHRETLKEVEKLKMDIRQLPESVIVETTEYKCLQSQFSVLYNESMQIKTMLDETRNQLQTSKNQHLRQIEVMESEELIAQKKVRSEMIQMEDVLALIRKEYETLRIEFEQNMAANEQTAPINREMRHLITSLQNHNGQLKGEVQRYKRKYKDTSTDNLKLRQELADALATLEGNKLQAATGAAGEEIKQENSTGVKEENSNNVSASGQTNQTNSGNDTNVAIKEENHISAEDEADDEASGKDVKDGIKQEKLSSGDAAAAEKKDSPGPGNSTSSATNSVPVKNEKDSKDGVKGKDVKAVESETVRDLKAQLKKALNDQKEMKLLLDMYKGVSKDQRDKVQLMATEKKLRSEIEELRQQLKKLQESKREERKKLADEEALRKIKQLEEQKYELQKQMANHKPTDNSWGSGAPGTANYTRPFVGSHEEEALLNEMEVTGQAFEDMQEQNSRLIQQLREKDDANFKLMSERIKANQLHKLLREEKTVLEDQMATATTQIEAMHIVLRKLEEKERSLQATVASIEKELMLRQQAMEMHKRKAIESAQSAADLKLHLEKYHAQMKEAQQVVAEKTSSLEAEAYKTKRLQEELAQFKRKAERMKKMEMSGTTIDEVMIEEIREYKETLTCPSCKVKRKDAVLSKCFHVFCYDCLRTRYETRQRKCPKCNCAFGANDYHRLYLQ

Target ORF information:

RefSeq Version	NM_139732.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Bre1, transcript variant A (Bre1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139732.3

ORF Insert Sequence:

ATGTCGAAAC GGAGCGCCGA CGACGCCACC GGCAGCAGCT GCTTGGTGGC CGCCGCAGCC 
GCCGGCCAGC CGCCCATCAA GAAGGTGCAC TTCGAGCCCC ATCTGATTGG GCCGGTGTCC 
ACGCTGGAGG AGATGGACAT CAAGGTGCTG GAATTCCAGA ACAAGAAGCT AGCACAGCGC 
ATCGAACAGC GGATGCGAAC GGAGGCGGAG CTGCGACATC GAATCGAGCA GCTGGAAAAG 
CGGCAAACGC AGGACGACGC CGTTCTAAAC GTGGTCAATC GGTATTGGAA TCAGCTGAAC 
GAGGACATAC GAGTCCTGCT GCAGCGCTTC GATGCCGAGA CGGCCGACGA ACTGGAGAAC 
CGGAATGAGA ACGAGGTGAC CACCTCGTTT CTCGCCCAAC TGTCCACCTG GGACAAGGAG 
GAGCTGGACG AGAAGCTGGC CAACCGCGTC CAAGTGTCCA AAAGGGCGGT CGCCAAGATC 
GTACAGGTTA TTGATAGGTT AATGCAACGC AATGAAAAGA TAACTCATGT CTTGAAAGGT 
GATAGTCTGG CTTCAGCTGG CTCCGGTTCA GGAGCGGGAG CTGGCGGCGA GGAGGAGCAG 
CAGCAGGCCA GCGGCGATGC GGAGACCACA ACGTCATCGG CCGGTGTCCA TGCGCTAGAG 
GAAACCCTGA AGCAAACGCA CATCGAGATC ATGAGCGAAA ATCACAAGCT GCAGAATCTG 
AATACCTCGC TGCACGAGAA GTTCCACACC ATGTCGCTGA AGATGAAGGA GTACCAGGAC 
GCTCATACCG CCAAGGAGAC GGAGAACGCC GAACTGAAGA ACCAGATAGA CGAACTGCAA 
TATGACCTAG AGAAGATCCA TTGCCGGAAT GATAAGCTGG AGAACCACTT GGCCGAGGCT 
ATTGAAAAGC TGAAGGCCTA CCACCAGATT TACGGTGATC CCAATAAGAG CACTAATAGT 
GCCAAGACAC CAACTACCAC AGGCAGCGGC GGCGCCACCA CCAGCGTTAA TAGCCAGCTA 
TTGGAGGAGC TGCAAAAGGA GCTGGAGGAG TACCGCGAAC TGGCCAACAA CCGACTGCAG 
GAGCTGGATA AGCTTCACGC CACGCATCGC GAAACCCTGA AGGAGGTGGA GAAGCTTAAA 
ATGGATATAC GACAGTTGCC GGAATCGGTA ATTGTGGAAA CCACGGAGTA CAAGTGCCTG 
CAGAGCCAGT TTTCCGTGCT CTACAACGAG TCCATGCAGA TAAAGACCAT GCTGGACGAG 
ACCCGAAACC AGCTTCAAAC GAGCAAGAAC CAGCATCTGC GACAAATCGA AGTGATGGAG 
AGCGAGGAAC TTATTGCCCA GAAGAAGGTG CGCAGCGAGA TGATCCAGAT GGAGGACGTT 
TTAGCCCTCA TCCGAAAGGA GTACGAAACG CTACGCATTG AGTTCGAGCA GAACATGGCA 
GCCAACGAGC AGACGGCACC CATCAATCGC GAGATGCGCC ACCTGATCAC CTCTCTACAA 
AACCACAATG GCCAACTGAA GGGTGAGGTG CAGCGCTACA AGCGAAAGTA CAAGGACACG 
TCCACGGACA ACTTGAAGCT GCGTCAGGAG CTGGCCGATG CACTAGCTAC ACTCGAAGGC 
AACAAGTTGC AGGCGGCGAC TGGTGCGGCT GGCGAAGAAA TCAAGCAGGA GAACTCAACA 
GGTGTGAAGG AGGAGAACTC CAACAACGTT TCCGCCAGTG GACAAACAAA CCAAACAAAT 
TCCGGCAACG ATACCAACGT AGCCATCAAG GAGGAGAACC ATATCTCGGC TGAAGATGAA 
GCTGATGACG AGGCCAGTGG TAAGGACGTA AAAGACGGCA TCAAGCAGGA GAAGCTCAGT 
TCTGGAGACG CGGCGGCAGC TGAGAAGAAG GATTCCCCTG GACCCGGAAA CTCAACAAGT 
TCTGCGACCA ATTCTGTTCC CGTCAAAAAC GAAAAGGATT CCAAGGATGG TGTCAAGGGC 
AAGGATGTGA AGGCAGTGGA AAGTGAAACG GTGCGCGATC TGAAGGCGCA ACTAAAAAAA 
GCACTGAACG ACCAGAAAGA AATGAAACTC CTGCTGGATA TGTACAAAGG CGTATCGAAG 
GATCAGCGCG ACAAGGTCCA GTTGATGGCC ACCGAAAAGA AACTGCGCTC CGAGATCGAG 
GAGCTCCGCC AGCAGCTGAA GAAGCTGCAG GAAAGCAAGC GCGAGGAACG CAAGAAGCTG 
GCCGACGAGG AGGCGTTACG TAAGATTAAG CAATTGGAGG AGCAGAAGTA CGAGCTGCAA 
AAACAGATGG CCAACCACAA GCCAACGGAT AATTCTTGGG GCAGTGGAGC CCCCGGCACA 
GCGAACTACA CGCGACCGTT TGTTGGTTCC CACGAAGAGG AGGCACTGCT GAACGAGATG 
GAGGTTACCG GCCAGGCGTT CGAGGACATG CAGGAGCAGA ACTCCCGGCT CATACAACAG 
CTGCGTGAGA AGGACGATGC GAACTTTAAG CTAATGTCGG AGCGCATCAA GGCCAACCAG 
CTGCACAAAC TTCTGAGAGA GGAAAAGACG GTGCTGGAGG ATCAGATGGC AACGGCGACC 
ACGCAAATCG AAGCCATGCA CATTGTGCTG CGAAAGCTGG AGGAGAAGGA ACGCAGCCTG 
CAGGCCACCG TCGCGTCGAT TGAGAAAGAG TTGATGCTGC GGCAGCAAGC AATGGAGATG 
CACAAACGCA AGGCGATTGA ATCGGCGCAA TCGGCAGCGG ACCTCAAGCT GCATCTGGAA 
AAGTATCACG CGCAAATGAA GGAGGCGCAG CAAGTGGTGG CGGAGAAGAC CAGTTCGCTG 
GAGGCGGAGG CCTACAAGAC CAAGCGGCTG CAGGAGGAGC TGGCGCAGTT TAAACGCAAG 
GCGGAGCGCA TGAAGAAGAT GGAAATGTCG GGCACCACCA TCGACGAGGT GATGATTGAG 
GAGATCCGCG AATACAAAGA AACGCTCACG TGTCCCTCGT GCAAGGTGAA GCGGAAGGAT 
GCTGTCCTGT CAAAGTGTTT CCACGTCTTC TGCTACGACT GCCTGCGCAC TAGATATGAA 
ACGCGGCAGC GGAAGTGTCC CAAATGCAAC TGCGCCTTCG GCGCCAACGA TTATCATAGG 
CTATATCTGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Bre1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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