Klp64D cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Klp64D
Entrez Gene ID	38611
Full Name	Kinesin-like protein at 64D
Synonyms	CG10642,DmKlp64D,Dmel\CG10642,KIF 3A,KIF3A,KLP4,KLP64D,KLP64Ddm,Kif3A,Klp 64D,Klp4,kif3A,klp64D
General protein information	Preferred Names Kinesin-like protein at 64D Names CG10642 gene product from transcript CG10642-RA CG10642-PA Kinesin-2 Kinesin2B Klp64D-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 64C13-64C13

mRNA and Protein(s)

mRNA	Protein	Name
NM_079210.3	NP_523934.1	Kinesin-like protein at 64D

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Kif3a	NP_445829.1
Gallus gallus (chicken)	KIF3A	NP_001025793.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002427	XP_312517.5
Bos taurus (cattle)	KIF3A	NP_001193783.1
Danio rerio (zebrafish)	kif3a	NP_001017604.2
Danio rerio (zebrafish)	LOC100537981	XP_003199933.1
Xenopus tropicalis (tropical clawed frog)	kif3a	XP_004912887.1
Homo sapiens (human)	KIF3A	NP_008985.3
Pan troglodytes (chimpanzee)	KIF3A	NP_001233450.1
	KIF3A	XP_001099789.2
Canis lupus familiaris (dog)	KIF3A	XP_860862.2
Mus musculus (house mouse)	Kif3a	NP_032469.2
Drosophila melanogaster (fruit fly)	Klp64D	NP_523934.1
Caenorhabditis elegans (roundworm)	klp-20	NP_497178.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Klp64D is required for wing margin development. Reduced Klp64D function is suppressed by activated Arm.
Title: Kinesin-II recruits Armadillo and Dishevelled for Wingless signaling in Drosophila.

KLP64D tail binds to endogenous ChAT
Title: Interaction with a kinesin-2 tail propels choline acetyltransferase flow towards synapse.

KLP64D and KLP68D Stalks Heterodimerize through the C-terminal 1/3rd Segments, and the Association is Stabilized by the N-terminal Segments.
Title: Biochemical and molecular dynamic simulation analysis of a weak coiled coil association between kinesin-II stalks.

Data show that kinesin-2, and the +TIPS EB1 and APC, are required for uniform dendrite microtubule polarity.
Title: Directed microtubule growth, +TIPs, and kinesin-2 are required for uniform microtubule polarity in dendrites.

Klp64D function is essential for Bazooka (Par-3 homolog) and Armadillo localization to the adherens junction (AJ) in pupal photoreceptors.
Title: Kinesin II is required for cell survival and adherens junction positioning in Drosophila photoreceptors.

The following Klp64D gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Klp64D cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa17583	NM_079210.3 Latest version!	Drosophila melanogaster Kinesin-like protein at 64D (Klp64D), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Klp64D ( NM_079210.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523934.1 Drosophila melanogaster Kinesin-like protein at 64D (Klp64D), mRNA.

Price & Availability↑

CloneID	OFa17583
Clone ID Related Accession (Same CDS sequence)	NM_079210.3
Accession Version	NM_079210.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Kinesin-like protein at 64D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_079210.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGCAGG AGGAGGCAAA TGCGGGAACA ACGGCCCAAC TCGATGACGA AATCGAGAAT 
GTGCGTGTGG TGGTGCGAAC GCGACCCATG GACAAAAATG AGTTGTCCGC GGGAGCGCTA 
AGTGCGATTT CGGTGGATAA GATCAACCGG GCCATTACGG TGATGAAACC GAATGCCACC 
GCCAATGAAC CGCCGAAAAC GTACTACTTC GACAATGTAT TCGATGGTGG CTCCAATCAA 
ATGGATCTCT ATGTGGACAC CGCTCGTCCG ATTGTGGACA AGGTGCTGGA GGGCTATAAT 
GGCACCATCT TGGCCTACGG ACAAACGGGC ACCGGCAAGA CGTATACCAT GTCGGGGAAT 
CCGGATTCGC CGCAAACAAA GGGCATCATT CCGAATGCAT TTGCGCACAT CTTTGGTCAC 
ATTGCTAAGG CCAAGGAGAA TCAGAAGTTC CTGGTGCGCG TCAGCTACAT GGAGATCTAT 
AACGAAGAGG TGCGGGATCT ACTGGGCAAG GATGTGGGCA AGAGCCTGGA GGTCAAGGAG 
CGACCGGACA TCGGTGTCTT TGTTAAGGAT CTCAGTGGCT ATGTGGTGCA CAACGCCGAT 
GATCTGGAGA ACATCATGCG TTTGGGTAAC AAAAATCGTG CCGTGGGAGC CACCAAAATG 
AACCAGGAGA GCTCCCGCTC CCATGCCATT TTCTCCATTA CTGTGGAGAG AAGTGAGCTG 
GGCGAAGGTG ATGTCCAGCA CGTAAGGATG GGTAAACTAC AGCTGGTGGA TCTCGCCGGC 
TCCGAAAGGC AGTCGAAGAC CCAGGCCAGT GGACAGAGAC TTAAAGAGGC CACCAAGATT 
AATCTTTCGC TATCGGTGCT GGGAAATGTG ATCAGTGCTT TGGTGGATGG AAAAAGCACC 
CACATACCGT ATCGGAACTC CAAGTTAACT CGACTTCTGC AGGACTCCCT CGGTGGTAAC 
TCCAAGACCG TCATGTGTGC CACCATTAGT CCAGCGGATA GCAATTATAT GGAAACTATA 
TCCACACTTC GCTATGCTAG TCGTGCTAAG AATATCCAAA ATCGCATGCA CATCAATGAG 
GAACCCAAGG ATGCTTTGCT TCGTCACTTT CAGGAGGAGA TTGCCCGACT TCGCAAGCAA 
TTGGAGGAGG GCGACAGCTT GGAGGAGGAG CCACCGAGCT CCGAAGAGGA GGAGGATACA 
GCAGATGACG AACTGGAGGC ACCACTGGAG ATCGAATTGG AATCATCAAC GATCCAGGCT 
GTGGAGAAAA AACCCAAGAA GAAGCGCGAG AAAACCGATG CCGAAAAGGA GGAACTGGCC 
AAGCGCAAAA ACGAGCATCA GAAGGAAATC GAGCATGCTA AGACGGAGCA GGAAACACTT 
CGCAATAAAC TAGTCTCATT GGAAGGTAAA ATTCTCGTTG GCGGCGAGAA TCTGCTGGAG 
AAAGCCCAGA CGCAAGAGCT GCTGCTAGAG CAATCCATTG CGGAGCTGGA ACAACATGAA 
AAGTCCGAGG AGGCGCTGAA GCAAACTCTA CAGCAAAAGG CCACCGAAAG GATTGACATT 
GAGGAGCGTT ACTCCACGCT TCAAGATGCC AGCACTGGCA TTACCAAGAA GATACACCGG 
GTGATGCAGA TGCTGATGGG CGTGAAATCG GAGCTGGCGG ATCAGCAGCA GGAGCACCAG 
CGCGAAAAGG AGGGCATATA CGAGAATATC AGGAGCCTAT CCAGGGAATT GGCCTTATGT 
GAGCTGGTAT TAAATTCCTA TGTGCCCAAG GAGTACCAAT CGATGATTAA TCAGTATACG 
CACTGGAACG AGGACATCGG CGAGTGGCAG CTGAAGTGTG TGGCCTACAC GGGTAACAAT 
ATGAGGAAAC ACATCTCGGC GCACAAGACG AGTGGCAAGG AGCCAGATTT CCTCGATCTC 
TCACATGTCT ATCTCAGCTA CAACACCGAT GGCGTGTCCA ATCCAATGCG CTCCAAATCC 
GCCAGGCCGC GAACCTCGGG TGTGCCCAGA CCGACGACTG CCAGGCGATA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523934.1
CDS	139..2172
Translation	MPQEEANAGTTAQLDDEIENVRVVVRTRPMDKNELSAGALSAISVDKINRAITVMKPNATANEPPKTYYFDNVFDGGSNQMDLYVDTARPIVDKVLEGYNGTILAYGQTGTGKTYTMSGNPDSPQTKGIIPNAFAHIFGHIAKAKENQKFLVRVSYMEIYNEEVRDLLGKDVGKSLEVKERPDIGVFVKDLSGYVVHNADDLENIMRLGNKNRAVGATKMNQESSRSHAIFSITVERSELGEGDVQHVRMGKLQLVDLAGSERQSKTQASGQRLKEATKINLSLSVLGNVISALVDGKSTHIPYRNSKLTRLLQDSLGGNSKTVMCATISPADSNYMETISTLRYASRAKNIQNRMHINEEPKDALLRHFQEEIARLRKQLEEGDSLEEEPPSSEEEEDTADDELEAPLEIELESSTIQAVEKKPKKKREKTDAEKEELAKRKNEHQKEIEHAKTEQETLRNKLVSLEGKILVGGENLLEKAQTQELLLEQSIAELEQHEKSEEALKQTLQQKATERIDIEERYSTLQDASTGITKKIHRVMQMLMGVKSELADQQQEHQREKEGIYENIRSLSRELALCELVLNSYVPKEYQSMINQYTHWNEDIGEWQLKCVAYTGNNMRKHISAHKTSGKEPDFLDLSHVYLSYNTDGVSNPMRSKSARPRTSGVPRPTTARRY

Target ORF information:

RefSeq Version	NM_079210.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Kinesin-like protein at 64D (Klp64D), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079210.3

ORF Insert Sequence:

ATGCCGCAGG AGGAGGCAAA TGCGGGAACA ACGGCCCAAC TCGATGACGA AATCGAGAAT 
GTGCGTGTGG TGGTGCGAAC GCGACCCATG GACAAAAATG AGTTGTCCGC GGGAGCGCTA 
AGTGCGATTT CGGTGGATAA GATCAACCGG GCCATTACGG TGATGAAACC GAATGCCACC 
GCCAATGAAC CGCCGAAAAC GTACTACTTC GACAATGTAT TCGATGGTGG CTCCAATCAA 
ATGGATCTCT ATGTGGACAC CGCTCGTCCG ATTGTGGACA AGGTGCTGGA GGGCTATAAT 
GGCACCATCT TGGCCTACGG ACAAACGGGC ACCGGCAAGA CGTATACCAT GTCGGGGAAT 
CCGGATTCGC CGCAAACAAA GGGCATCATT CCGAATGCAT TTGCGCACAT CTTTGGTCAC 
ATTGCTAAGG CCAAGGAGAA TCAGAAGTTC CTGGTGCGCG TCAGCTACAT GGAGATCTAT 
AACGAAGAGG TGCGGGATCT ACTGGGCAAG GATGTGGGCA AGAGCCTGGA GGTCAAGGAG 
CGACCGGACA TCGGTGTCTT TGTTAAGGAT CTCAGTGGCT ATGTGGTGCA CAACGCCGAT 
GATCTGGAGA ACATCATGCG TTTGGGTAAC AAAAATCGTG CCGTGGGAGC CACCAAAATG 
AACCAGGAGA GCTCCCGCTC CCATGCCATT TTCTCCATTA CTGTGGAGAG AAGTGAGCTG 
GGCGAAGGTG ATGTCCAGCA CGTAAGGATG GGTAAACTAC AGCTGGTGGA TCTCGCCGGC 
TCCGAAAGGC AGTCGAAGAC CCAGGCCAGT GGACAGAGAC TTAAAGAGGC CACCAAGATT 
AATCTTTCGC TATCGGTGCT GGGAAATGTG ATCAGTGCTT TGGTGGATGG AAAAAGCACC 
CACATACCGT ATCGGAACTC CAAGTTAACT CGACTTCTGC AGGACTCCCT CGGTGGTAAC 
TCCAAGACCG TCATGTGTGC CACCATTAGT CCAGCGGATA GCAATTATAT GGAAACTATA 
TCCACACTTC GCTATGCTAG TCGTGCTAAG AATATCCAAA ATCGCATGCA CATCAATGAG 
GAACCCAAGG ATGCTTTGCT TCGTCACTTT CAGGAGGAGA TTGCCCGACT TCGCAAGCAA 
TTGGAGGAGG GCGACAGCTT GGAGGAGGAG CCACCGAGCT CCGAAGAGGA GGAGGATACA 
GCAGATGACG AACTGGAGGC ACCACTGGAG ATCGAATTGG AATCATCAAC GATCCAGGCT 
GTGGAGAAAA AACCCAAGAA GAAGCGCGAG AAAACCGATG CCGAAAAGGA GGAACTGGCC 
AAGCGCAAAA ACGAGCATCA GAAGGAAATC GAGCATGCTA AGACGGAGCA GGAAACACTT 
CGCAATAAAC TAGTCTCATT GGAAGGTAAA ATTCTCGTTG GCGGCGAGAA TCTGCTGGAG 
AAAGCCCAGA CGCAAGAGCT GCTGCTAGAG CAATCCATTG CGGAGCTGGA ACAACATGAA 
AAGTCCGAGG AGGCGCTGAA GCAAACTCTA CAGCAAAAGG CCACCGAAAG GATTGACATT 
GAGGAGCGTT ACTCCACGCT TCAAGATGCC AGCACTGGCA TTACCAAGAA GATACACCGG 
GTGATGCAGA TGCTGATGGG CGTGAAATCG GAGCTGGCGG ATCAGCAGCA GGAGCACCAG 
CGCGAAAAGG AGGGCATATA CGAGAATATC AGGAGCCTAT CCAGGGAATT GGCCTTATGT 
GAGCTGGTAT TAAATTCCTA TGTGCCCAAG GAGTACCAAT CGATGATTAA TCAGTATACG 
CACTGGAACG AGGACATCGG CGAGTGGCAG CTGAAGTGTG TGGCCTACAC GGGTAACAAT 
ATGAGGAAAC ACATCTCGGC GCACAAGACG AGTGGCAAGG AGCCAGATTT CCTCGATCTC 
TCACATGTCT ATCTCAGCTA CAACACCGAT GGCGTGTCCA ATCCAATGCG CTCCAAATCC 
GCCAGGCCGC GAACCTCGGG TGTGCCCAGA CCGACGACTG CCAGGCGATA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Klp64D cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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