Hsp83 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hsp83
Entrez Gene ID	38389
Full Name	Heat shock protein 83
Synonyms	143198_at,83,83K HSP,CG1242,DMHSP82,DmHsp83,Dmel\CG1242,E(sev)3A,E(sina)2,HSP82,HSP83,HSP90,HSP90A1,Hsp82,Hsp90,ORF1,P02828,Su(Raf)3A,anon-EST:Liang-2.53,anon-WO0068693,anon-WO0140519.209,clone 2.53,en(lz)3C/4C,hsp82,hsp83,hsp84,hsp90,l(3)j5C2,ms(3)08445,stc
General protein information	Preferred Names Heat shock protein 83 Names CG1242 gene product from transcript CG1242-RB CG1242-PA CG1242-PB Hsp83-PA Hsp83-PB enhancer of seven in absentia 2 enhancer of sevenless 3A heat shock protein 83 heat-shock protein 90 kDa scratch
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 63B11-63B11\|3-5 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001274433.1	NP_001261362.1	heat shock protein 83, isoform B
NM_079175.4	NP_523899.1	heat shock protein 83, isoform A

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Hsp83	NP_001261362.1
Arabidopsis thaliana (thale cress)	HSP90.1	NP_200076.1
Mus musculus (house mouse)	Hsp90ab1	NP_032328.2
Gallus gallus (chicken)	HSP90AB1	NP_996842.1
Saccharomyces cerevisiae (baker's yeast)	HSP82	NP_015084.1
Kluyveromyces lactis	KLLA0D12958g	XP_453640.1
Schizosaccharomyces pombe (fission yeast)	hsp90	NP_594365.1
Canis lupus familiaris (dog)	HSP90AB1	XP_532154.4
Bos taurus (cattle)	HSP90AB1	NP_001073105.1
Danio rerio (zebrafish)	hsp90ab1	NP_571385.2
Caenorhabditis elegans (roundworm)	daf-21	NP_506626.1
Neurospora crassa	NCU04142	XP_961298.1
Oryza sativa (rice)	Os08g0500700	NP_001062159.1
Homo sapiens (human)	HSP90AB1	NP_001258898.1
Pan troglodytes (chimpanzee)	HSP90AB1	XP_003311385.1
	HSP90AB1	XP_001099135.2
Rattus norvegicus (Norway rat)	Hsp90ab1	NP_001004082.3
Anopheles gambiae (African malaria mosquito)	HSP83_ANOGA	XP_308800.3
Eremothecium gossypii	AGOS_AFL148C	NP_985402.1
Magnaporthe oryzae (rice blast fungus)	MGG_06759	XP_003709473.1
Xenopus tropicalis (tropical clawed frog)	hsp90ab1	NP_001025655.1
Saccharomyces cerevisiae (baker's yeast)	HSC82	NP_013911.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Nup358 facilitates JH-induced Met nuclear transport in a manner dependent on importin beta and Hsp83.
Title: Nucleoporin Nup358 facilitates nuclear import of Methoprene-tolerant (Met) in an importin ?- and Hsp83-dependent manner.

We show that while Hsp70 or Hsp83 expression under normal or stress conditions was not affected by AR feeding, Hsp27 levels were elevated in AR-fed wild-type control as well as heat-shocked larvae
Title: Ayurvedic

Escargot and Scratch regulate neural commitment by antagonizing Notch activity in Drosophila sensory organs
Title: Escargot and Scratch regulate neural commitment by antagonizing Notch activity in Drosophila sensory organs.

The results revealed that the high-fat diet augmented the rate of lipid peroxidation and SOD and CAT activity and induced a higher expression of HSP83 and MPK2 mRNA.
Title: High-Fat Diet Induces Oxidative Stress and MPK2 and HSP83 Gene Expression in Drosophila melanogaster.

Hsp83 facilitates methoprene-tolerant nuclear import to modulate juvenile hormone signaling.
Title: Heat shock protein 83 (Hsp83) facilitates methoprene-tolerant (Met) nuclear import to modulate juvenile hormone signaling.

The following Hsp83 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hsp83 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa17355	NM_079175.4 Latest version!	Drosophila melanogaster heat shock protein 83, transcript variant A (Hsp83), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa17355	NM_001274433.1 Latest version!	Drosophila melanogaster heat shock protein 83, transcript variant B (Hsp83), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hsp83 ( NM_001274433.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261362.1 Drosophila melanogaster heat shock protein 83, transcript variant B (Hsp83), mRNA.

Price & Availability↑

CloneID	OFa17355
Clone ID Related Accession (Same CDS sequence)	NM_001274433.1 , NM_079175.4
Accession Version	NM_001274433.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	heat shock protein 83, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCAGAAG AAGCAGAGAC CTTTGCATTC CAGGCTGAGA TTGCTCAGCT GATGTCCCTG 
ATCATCAACA CATTCTACTC GAACAAGGAG ATTTTCCTGC GCGAGTTGAT CTCGAACGCT 
TCCGATGCCC TGGACAAGAT CCGCTATGAG TCCCTTACTG ACCCCAGCAA GCTGGACTCT 
GGCAAGGAGC TGTACATCAA GCTGATCCCT AACAAGACGG CTGGTACTCT GACCATCATT 
GATACCGGTA TCGGTATGAC CAAGTCCGAC CTGGTCAACA ACTTGGGAAC CATTGCCAAG 
TCCGGAACCA AGGCCTTCAT GGAGGCTCTG CAGGCTGGTG CCGACATTTC CATGATCGGT 
CAGTTCGGTG TGGGTTTCTA CTCCGCCTAC CTGGTCGCCG ACAAGGTGAC TGTCACCTCC 
AAGAACAACG ATGACGAGCA GTACGTGTGG GAGTCCTCTG CCGGAGGCTC TTTCACAGTC 
CGTGCCGACA ACTCTGAGCC CCTGGGCCGT GGCACCAAGA TCGTGCTGTA CATCAAGGAG 
GACCAGACCG ACTATCTGGA GGAGAGCAAG ATCAAGGAGA TTGTTAACAA GCACTCCCAG 
TTCATTGGCT ACCCCATCAA GCTGCTCGTA GAGAAGGAGC GCGAGAAGGA GGTCAGCGAC 
GATGAGGCTG ATGATGAGAA GAAGGAAGGT GATGAGAAGA AGGAGATGGA GACTGATGAG 
CCCAAAATCG AGGATGTTGG CGAGGATGAG GATGCCGACA AGAAGGACAA GGATGCCAAG 
AAGAAGAAGA CCATCAAGGA GAAGTACACT GAGGATGAGG AGCTGAACAA GACCAAGCCC 
ATCTGGACCC GCAATCCCGA TGATATCTCC CAGGAGGAGT ACGGCGAGTT CTACAAATCC 
CTGACCAACG ACTGGGAGGA TCATCTGGCC GTCAAGCACT TCTCCGTGGA GGGTCAGCTG 
GAGTTCCGTG CTCTGCTCTT CATTCCCCGT CGCACGCCCT TCGATCTCTT CGAGAACCAG 
AAGAAGCGCA ACAACATCAA GCTGTACGTG CGTCGTGTCT TCATCATGGA CAACTGCGAG 
GACCTCATTC CAGAGTACTT GAACTTCATG AAGGGTGTGG TCGACTCCGA GGATCTGCCC 
CTCAACATCT CACGTGAGAT GCTGCAGCAG AACAAGGTCC TAAAGGTGAT CCGCAAGAAC 
CTGGTCAAGA AGACCATGGA GCTGATTGAG GAGCTCACCG AGGACAAGGA GAACTACAAG 
AAGTTCTATG ACCAGTTCAG CAAGAACCTG AAGCTGGGTG TGCACGAGGA CAGCAACAAC 
CGTGCCAAGT TGGCCGACTT CCTTCGCTTC CACACCTCTG CCTCCGGCGA CGATTTCTGC 
TCCCTGGCCG ACTACGTGTC GCGCATGAAG GATAACCAGA AGCACGTGTA CTTCATCACT 
GGCGAGTCCA AGGACCAGGT CAGCAACTCT GCCTTCGTGG AGCGCGTCAA GGCCCGTGGC 
TTCGAGGTGG TCTACATGAC CGAGCCCATC GATGAGTATG TCATCCAGCA CTTGAAGGAG 
TACAAGGGCA AGCAGCTGGT CTCTGTCACC AAGGAGGGTC TGGAGCTGCC TGAGGATGAG 
AGCGAGAAGA AGAAGCGCGA GGAGGACAAG GCCAAGTTCG AGAGCCTGTG CAAGCTGATG 
AAGTCCATCC TGGACAACAA GGTCGAGAAG GTGGTGGTGT CCAACCGCCT GGTGGATTCG 
CCCTGCTGCA TTGTCACTTC GCAGTTCGGC TGGTCCGCTA ACATGGAGCG CATCATGAAG 
GCCCAGGCTC TGCGTGATAC CGCCACAATG GGCTACATGG CCGGCAAGAA GCAGCTGGAG 
ATCAACCCCG ATCACCCAAT TGTGGAGACT CTCCGCCAGA AGGCCGATGC CGACAAGAAC 
GATAAGGCCG TCAAGGATCT GGTCATCCTG CTGTTCGAGA CCTCTCTGCT GTCCTCTGGA 
TTCTCGCTGG ACAGCCCCCA GGTGCACGCC AGCCGCATCT ACCGCATGAT CAAGCTGGGC 
TTGGGAATCG ACGAGGACGA GCCTATGACT ACCGACGATG CCCAGAGCGC CGGAGATGCC 
CCCTCGCTGG TTGAGGACAC AGAGGACGCT TCCCACATGG AGGAGGTCGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261362.1
CDS	400..2553
Translation	MPEEAETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNASDALDKIRYESLTDPSKLDSGKELYIKLIPNKTAGTLTIIDTGIGMTKSDLVNNLGTIAKSGTKAFMEALQAGADISMIGQFGVGFYSAYLVADKVTVTSKNNDDEQYVWESSAGGSFTVRADNSEPLGRGTKIVLYIKEDQTDYLEESKIKEIVNKHSQFIGYPIKLLVEKEREKEVSDDEADDEKKEGDEKKEMETDEPKIEDVGEDEDADKKDKDAKKKKTIKEKYTEDEELNKTKPIWTRNPDDISQEEYGEFYKSLTNDWEDHLAVKHFSVEGQLEFRALLFIPRRTPFDLFENQKKRNNIKLYVRRVFIMDNCEDLIPEYLNFMKGVVDSEDLPLNISREMLQQNKVLKVIRKNLVKKTMELIEELTEDKENYKKFYDQFSKNLKLGVHEDSNNRAKLADFLRFHTSASGDDFCSLADYVSRMKDNQKHVYFITGESKDQVSNSAFVERVKARGFEVVYMTEPIDEYVIQHLKEYKGKQLVSVTKEGLELPEDESEKKKREEDKAKFESLCKLMKSILDNKVEKVVVSNRLVDSPCCIVTSQFGWSANMERIMKAQALRDTATMGYMAGKKQLEINPDHPIVETLRQKADADKNDKAVKDLVILLFETSLLSSGFSLDSPQVHASRIYRMIKLGLGIDEDEPMTTDDAQSAGDAPSLVEDTEDASHMEEVD

Target ORF information:

RefSeq Version	NM_001274433.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster heat shock protein 83, transcript variant B (Hsp83), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274433.1

ORF Insert Sequence:

ATGCCAGAAG AAGCAGAGAC CTTTGCATTC CAGGCTGAGA TTGCTCAGCT GATGTCCCTG 
ATCATCAACA CATTCTACTC GAACAAGGAG ATTTTCCTGC GCGAGTTGAT CTCGAACGCT 
TCCGATGCCC TGGACAAGAT CCGCTATGAG TCCCTTACTG ACCCCAGCAA GCTGGACTCT 
GGCAAGGAGC TGTACATCAA GCTGATCCCT AACAAGACGG CTGGTACTCT GACCATCATT 
GATACCGGTA TCGGTATGAC CAAGTCCGAC CTGGTCAACA ACTTGGGAAC CATTGCCAAG 
TCCGGAACCA AGGCCTTCAT GGAGGCTCTG CAGGCTGGTG CCGACATTTC CATGATCGGT 
CAGTTCGGTG TGGGTTTCTA CTCCGCCTAC CTGGTCGCCG ACAAGGTGAC TGTCACCTCC 
AAGAACAACG ATGACGAGCA GTACGTGTGG GAGTCCTCTG CCGGAGGCTC TTTCACAGTC 
CGTGCCGACA ACTCTGAGCC CCTGGGCCGT GGCACCAAGA TCGTGCTGTA CATCAAGGAG 
GACCAGACCG ACTATCTGGA GGAGAGCAAG ATCAAGGAGA TTGTTAACAA GCACTCCCAG 
TTCATTGGCT ACCCCATCAA GCTGCTCGTA GAGAAGGAGC GCGAGAAGGA GGTCAGCGAC 
GATGAGGCTG ATGATGAGAA GAAGGAAGGT GATGAGAAGA AGGAGATGGA GACTGATGAG 
CCCAAAATCG AGGATGTTGG CGAGGATGAG GATGCCGACA AGAAGGACAA GGATGCCAAG 
AAGAAGAAGA CCATCAAGGA GAAGTACACT GAGGATGAGG AGCTGAACAA GACCAAGCCC 
ATCTGGACCC GCAATCCCGA TGATATCTCC CAGGAGGAGT ACGGCGAGTT CTACAAATCC 
CTGACCAACG ACTGGGAGGA TCATCTGGCC GTCAAGCACT TCTCCGTGGA GGGTCAGCTG 
GAGTTCCGTG CTCTGCTCTT CATTCCCCGT CGCACGCCCT TCGATCTCTT CGAGAACCAG 
AAGAAGCGCA ACAACATCAA GCTGTACGTG CGTCGTGTCT TCATCATGGA CAACTGCGAG 
GACCTCATTC CAGAGTACTT GAACTTCATG AAGGGTGTGG TCGACTCCGA GGATCTGCCC 
CTCAACATCT CACGTGAGAT GCTGCAGCAG AACAAGGTCC TAAAGGTGAT CCGCAAGAAC 
CTGGTCAAGA AGACCATGGA GCTGATTGAG GAGCTCACCG AGGACAAGGA GAACTACAAG 
AAGTTCTATG ACCAGTTCAG CAAGAACCTG AAGCTGGGTG TGCACGAGGA CAGCAACAAC 
CGTGCCAAGT TGGCCGACTT CCTTCGCTTC CACACCTCTG CCTCCGGCGA CGATTTCTGC 
TCCCTGGCCG ACTACGTGTC GCGCATGAAG GATAACCAGA AGCACGTGTA CTTCATCACT 
GGCGAGTCCA AGGACCAGGT CAGCAACTCT GCCTTCGTGG AGCGCGTCAA GGCCCGTGGC 
TTCGAGGTGG TCTACATGAC CGAGCCCATC GATGAGTATG TCATCCAGCA CTTGAAGGAG 
TACAAGGGCA AGCAGCTGGT CTCTGTCACC AAGGAGGGTC TGGAGCTGCC TGAGGATGAG 
AGCGAGAAGA AGAAGCGCGA GGAGGACAAG GCCAAGTTCG AGAGCCTGTG CAAGCTGATG 
AAGTCCATCC TGGACAACAA GGTCGAGAAG GTGGTGGTGT CCAACCGCCT GGTGGATTCG 
CCCTGCTGCA TTGTCACTTC GCAGTTCGGC TGGTCCGCTA ACATGGAGCG CATCATGAAG 
GCCCAGGCTC TGCGTGATAC CGCCACAATG GGCTACATGG CCGGCAAGAA GCAGCTGGAG 
ATCAACCCCG ATCACCCAAT TGTGGAGACT CTCCGCCAGA AGGCCGATGC CGACAAGAAC 
GATAAGGCCG TCAAGGATCT GGTCATCCTG CTGTTCGAGA CCTCTCTGCT GTCCTCTGGA 
TTCTCGCTGG ACAGCCCCCA GGTGCACGCC AGCCGCATCT ACCGCATGAT CAAGCTGGGC 
TTGGGAATCG ACGAGGACGA GCCTATGACT ACCGACGATG CCCAGAGCGC CGGAGATGCC 
CCCTCGCTGG TTGAGGACAC AGAGGACGCT TCCCACATGG AGGAGGTCGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hsp83 ( NM_079175.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523899.1 Drosophila melanogaster heat shock protein 83, transcript variant A (Hsp83), mRNA.

Price & Availability↑

CloneID	OFa17355
Clone ID Related Accession (Same CDS sequence)	NM_001274433.1 , NM_079175.4
Accession Version	NM_079175.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	heat shock protein 83, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079175.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCAGAAG AAGCAGAGAC CTTTGCATTC CAGGCTGAGA TTGCTCAGCT GATGTCCCTG 
ATCATCAACA CATTCTACTC GAACAAGGAG ATTTTCCTGC GCGAGTTGAT CTCGAACGCT 
TCCGATGCCC TGGACAAGAT CCGCTATGAG TCCCTTACTG ACCCCAGCAA GCTGGACTCT 
GGCAAGGAGC TGTACATCAA GCTGATCCCT AACAAGACGG CTGGTACTCT GACCATCATT 
GATACCGGTA TCGGTATGAC CAAGTCCGAC CTGGTCAACA ACTTGGGAAC CATTGCCAAG 
TCCGGAACCA AGGCCTTCAT GGAGGCTCTG CAGGCTGGTG CCGACATTTC CATGATCGGT 
CAGTTCGGTG TGGGTTTCTA CTCCGCCTAC CTGGTCGCCG ACAAGGTGAC TGTCACCTCC 
AAGAACAACG ATGACGAGCA GTACGTGTGG GAGTCCTCTG CCGGAGGCTC TTTCACAGTC 
CGTGCCGACA ACTCTGAGCC CCTGGGCCGT GGCACCAAGA TCGTGCTGTA CATCAAGGAG 
GACCAGACCG ACTATCTGGA GGAGAGCAAG ATCAAGGAGA TTGTTAACAA GCACTCCCAG 
TTCATTGGCT ACCCCATCAA GCTGCTCGTA GAGAAGGAGC GCGAGAAGGA GGTCAGCGAC 
GATGAGGCTG ATGATGAGAA GAAGGAAGGT GATGAGAAGA AGGAGATGGA GACTGATGAG 
CCCAAAATCG AGGATGTTGG CGAGGATGAG GATGCCGACA AGAAGGACAA GGATGCCAAG 
AAGAAGAAGA CCATCAAGGA GAAGTACACT GAGGATGAGG AGCTGAACAA GACCAAGCCC 
ATCTGGACCC GCAATCCCGA TGATATCTCC CAGGAGGAGT ACGGCGAGTT CTACAAATCC 
CTGACCAACG ACTGGGAGGA TCATCTGGCC GTCAAGCACT TCTCCGTGGA GGGTCAGCTG 
GAGTTCCGTG CTCTGCTCTT CATTCCCCGT CGCACGCCCT TCGATCTCTT CGAGAACCAG 
AAGAAGCGCA ACAACATCAA GCTGTACGTG CGTCGTGTCT TCATCATGGA CAACTGCGAG 
GACCTCATTC CAGAGTACTT GAACTTCATG AAGGGTGTGG TCGACTCCGA GGATCTGCCC 
CTCAACATCT CACGTGAGAT GCTGCAGCAG AACAAGGTCC TAAAGGTGAT CCGCAAGAAC 
CTGGTCAAGA AGACCATGGA GCTGATTGAG GAGCTCACCG AGGACAAGGA GAACTACAAG 
AAGTTCTATG ACCAGTTCAG CAAGAACCTG AAGCTGGGTG TGCACGAGGA CAGCAACAAC 
CGTGCCAAGT TGGCCGACTT CCTTCGCTTC CACACCTCTG CCTCCGGCGA CGATTTCTGC 
TCCCTGGCCG ACTACGTGTC GCGCATGAAG GATAACCAGA AGCACGTGTA CTTCATCACT 
GGCGAGTCCA AGGACCAGGT CAGCAACTCT GCCTTCGTGG AGCGCGTCAA GGCCCGTGGC 
TTCGAGGTGG TCTACATGAC CGAGCCCATC GATGAGTATG TCATCCAGCA CTTGAAGGAG 
TACAAGGGCA AGCAGCTGGT CTCTGTCACC AAGGAGGGTC TGGAGCTGCC TGAGGATGAG 
AGCGAGAAGA AGAAGCGCGA GGAGGACAAG GCCAAGTTCG AGAGCCTGTG CAAGCTGATG 
AAGTCCATCC TGGACAACAA GGTCGAGAAG GTGGTGGTGT CCAACCGCCT GGTGGATTCG 
CCCTGCTGCA TTGTCACTTC GCAGTTCGGC TGGTCCGCTA ACATGGAGCG CATCATGAAG 
GCCCAGGCTC TGCGTGATAC CGCCACAATG GGCTACATGG CCGGCAAGAA GCAGCTGGAG 
ATCAACCCCG ATCACCCAAT TGTGGAGACT CTCCGCCAGA AGGCCGATGC CGACAAGAAC 
GATAAGGCCG TCAAGGATCT GGTCATCCTG CTGTTCGAGA CCTCTCTGCT GTCCTCTGGA 
TTCTCGCTGG ACAGCCCCCA GGTGCACGCC AGCCGCATCT ACCGCATGAT CAAGCTGGGC 
TTGGGAATCG ACGAGGACGA GCCTATGACT ACCGACGATG CCCAGAGCGC CGGAGATGCC 
CCCTCGCTGG TTGAGGACAC AGAGGACGCT TCCCACATGG AGGAGGTCGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523899.1
CDS	153..2306
Translation	MPEEAETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNASDALDKIRYESLTDPSKLDSGKELYIKLIPNKTAGTLTIIDTGIGMTKSDLVNNLGTIAKSGTKAFMEALQAGADISMIGQFGVGFYSAYLVADKVTVTSKNNDDEQYVWESSAGGSFTVRADNSEPLGRGTKIVLYIKEDQTDYLEESKIKEIVNKHSQFIGYPIKLLVEKEREKEVSDDEADDEKKEGDEKKEMETDEPKIEDVGEDEDADKKDKDAKKKKTIKEKYTEDEELNKTKPIWTRNPDDISQEEYGEFYKSLTNDWEDHLAVKHFSVEGQLEFRALLFIPRRTPFDLFENQKKRNNIKLYVRRVFIMDNCEDLIPEYLNFMKGVVDSEDLPLNISREMLQQNKVLKVIRKNLVKKTMELIEELTEDKENYKKFYDQFSKNLKLGVHEDSNNRAKLADFLRFHTSASGDDFCSLADYVSRMKDNQKHVYFITGESKDQVSNSAFVERVKARGFEVVYMTEPIDEYVIQHLKEYKGKQLVSVTKEGLELPEDESEKKKREEDKAKFESLCKLMKSILDNKVEKVVVSNRLVDSPCCIVTSQFGWSANMERIMKAQALRDTATMGYMAGKKQLEINPDHPIVETLRQKADADKNDKAVKDLVILLFETSLLSSGFSLDSPQVHASRIYRMIKLGLGIDEDEPMTTDDAQSAGDAPSLVEDTEDASHMEEVD

Target ORF information:

RefSeq Version	NM_079175.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster heat shock protein 83, transcript variant A (Hsp83), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079175.4

ORF Insert Sequence:

ATGCCAGAAG AAGCAGAGAC CTTTGCATTC CAGGCTGAGA TTGCTCAGCT GATGTCCCTG 
ATCATCAACA CATTCTACTC GAACAAGGAG ATTTTCCTGC GCGAGTTGAT CTCGAACGCT 
TCCGATGCCC TGGACAAGAT CCGCTATGAG TCCCTTACTG ACCCCAGCAA GCTGGACTCT 
GGCAAGGAGC TGTACATCAA GCTGATCCCT AACAAGACGG CTGGTACTCT GACCATCATT 
GATACCGGTA TCGGTATGAC CAAGTCCGAC CTGGTCAACA ACTTGGGAAC CATTGCCAAG 
TCCGGAACCA AGGCCTTCAT GGAGGCTCTG CAGGCTGGTG CCGACATTTC CATGATCGGT 
CAGTTCGGTG TGGGTTTCTA CTCCGCCTAC CTGGTCGCCG ACAAGGTGAC TGTCACCTCC 
AAGAACAACG ATGACGAGCA GTACGTGTGG GAGTCCTCTG CCGGAGGCTC TTTCACAGTC 
CGTGCCGACA ACTCTGAGCC CCTGGGCCGT GGCACCAAGA TCGTGCTGTA CATCAAGGAG 
GACCAGACCG ACTATCTGGA GGAGAGCAAG ATCAAGGAGA TTGTTAACAA GCACTCCCAG 
TTCATTGGCT ACCCCATCAA GCTGCTCGTA GAGAAGGAGC GCGAGAAGGA GGTCAGCGAC 
GATGAGGCTG ATGATGAGAA GAAGGAAGGT GATGAGAAGA AGGAGATGGA GACTGATGAG 
CCCAAAATCG AGGATGTTGG CGAGGATGAG GATGCCGACA AGAAGGACAA GGATGCCAAG 
AAGAAGAAGA CCATCAAGGA GAAGTACACT GAGGATGAGG AGCTGAACAA GACCAAGCCC 
ATCTGGACCC GCAATCCCGA TGATATCTCC CAGGAGGAGT ACGGCGAGTT CTACAAATCC 
CTGACCAACG ACTGGGAGGA TCATCTGGCC GTCAAGCACT TCTCCGTGGA GGGTCAGCTG 
GAGTTCCGTG CTCTGCTCTT CATTCCCCGT CGCACGCCCT TCGATCTCTT CGAGAACCAG 
AAGAAGCGCA ACAACATCAA GCTGTACGTG CGTCGTGTCT TCATCATGGA CAACTGCGAG 
GACCTCATTC CAGAGTACTT GAACTTCATG AAGGGTGTGG TCGACTCCGA GGATCTGCCC 
CTCAACATCT CACGTGAGAT GCTGCAGCAG AACAAGGTCC TAAAGGTGAT CCGCAAGAAC 
CTGGTCAAGA AGACCATGGA GCTGATTGAG GAGCTCACCG AGGACAAGGA GAACTACAAG 
AAGTTCTATG ACCAGTTCAG CAAGAACCTG AAGCTGGGTG TGCACGAGGA CAGCAACAAC 
CGTGCCAAGT TGGCCGACTT CCTTCGCTTC CACACCTCTG CCTCCGGCGA CGATTTCTGC 
TCCCTGGCCG ACTACGTGTC GCGCATGAAG GATAACCAGA AGCACGTGTA CTTCATCACT 
GGCGAGTCCA AGGACCAGGT CAGCAACTCT GCCTTCGTGG AGCGCGTCAA GGCCCGTGGC 
TTCGAGGTGG TCTACATGAC CGAGCCCATC GATGAGTATG TCATCCAGCA CTTGAAGGAG 
TACAAGGGCA AGCAGCTGGT CTCTGTCACC AAGGAGGGTC TGGAGCTGCC TGAGGATGAG 
AGCGAGAAGA AGAAGCGCGA GGAGGACAAG GCCAAGTTCG AGAGCCTGTG CAAGCTGATG 
AAGTCCATCC TGGACAACAA GGTCGAGAAG GTGGTGGTGT CCAACCGCCT GGTGGATTCG 
CCCTGCTGCA TTGTCACTTC GCAGTTCGGC TGGTCCGCTA ACATGGAGCG CATCATGAAG 
GCCCAGGCTC TGCGTGATAC CGCCACAATG GGCTACATGG CCGGCAAGAA GCAGCTGGAG 
ATCAACCCCG ATCACCCAAT TGTGGAGACT CTCCGCCAGA AGGCCGATGC CGACAAGAAC 
GATAAGGCCG TCAAGGATCT GGTCATCCTG CTGTTCGAGA CCTCTCTGCT GTCCTCTGGA 
TTCTCGCTGG ACAGCCCCCA GGTGCACGCC AGCCGCATCT ACCGCATGAT CAAGCTGGGC 
TTGGGAATCG ACGAGGACGA GCCTATGACT ACCGACGATG CCCAGAGCGC CGGAGATGCC 
CCCTCGCTGG TTGAGGACAC AGAGGACGCT TCCCACATGG AGGAGGTCGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Hsp83 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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