Girdin cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Girdin
Entrez Gene ID	38385
Full Name	CG12734 gene product from transcript CG12734-RA
Synonyms	CG12734,Dmel\CG12734,mecA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 63B9-63B10

mRNA and Protein(s)

mRNA	Protein	Name
NM_206262.3	NP_995984.1	girdin, isoform B
NM_139523.3	NP_647780.1	girdin, isoform A
NM_001274430.1	NP_001261359.1	girdin, isoform D
NM_001274431.1	NP_001261360.1	girdin, isoform E
NM_001274429.1	NP_001261358.1	girdin, isoform C

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Girdin	NP_647780.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002527	XP_312411.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Girdin functions temporarily within the sensory organ and that this function is essential for the formation of the sensory dendrites via actin structures
Title: Drosophila Hook-Related Protein (Girdin) Is Essential for Sensory Dendrite Formation.

Studies indicate that knockdown of dGirdin in the Drosophila wing imaginal disc cells resulted in reduction of cell size and this was enhanced by half reduction of the Akt gene dose.
Title: dGirdin a new player of Akt /PKB signaling in Drosophila Melanogaster.

The following Girdin gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Girdin cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa17353	NM_001274430.1 Latest version!	Drosophila melanogaster girdin, transcript variant D (Girdin), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa20529	NM_206262.3 Latest version!	Drosophila melanogaster girdin, transcript variant B (Girdin), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa17352	NM_001274429.1 Latest version!	Drosophila melanogaster girdin, transcript variant C (Girdin), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa17354	NM_001274431.1 Latest version!	Drosophila melanogaster girdin, transcript variant E (Girdin), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa20528	NM_139523.3 Latest version!	Drosophila melanogaster girdin, transcript variant A (Girdin), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Girdin ( NM_001274430.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261359.1 Drosophila melanogaster girdin, transcript variant D (Girdin), mRNA.

Price & Availability↑

CloneID	OFa17353
Clone ID Related Accession (Same CDS sequence)	NM_001274430.1
Accession Version	NM_001274430.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	girdin, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTGGCA CTGCGACCGC GACGCCAATG GAAATTGATG AGTTTGTTAA TTCGTCCATA 
ATTTCTTGGC TGGAATCATG TCTGCCACGT GCCGAGCTTC TCGCCGGATA CACCTCCCTG 
CTGGATGGCC ACATAATTCA CAGCGTCTGG CTGCAAATCG ACCCCGAGCC GCAAAATAAT 
CCCAGCGAGC TGAACGATCT TAACGGGAAA TCTCTCAGCA TTGCGAGGGC CAAGAACTTC 
GAGTGCGTCG TGAGGAATCT AAAGTCTTTC TTTGAGGAGG AGCTTGGTCA AACCATACTG 
GTCCTACCCG ATGCCTTCAC CCTGGGTCAT CATCCGGAGA GTAAGAACGG GCTCGAACAG 
ATGAAGACCT TGCTGACCCT GTTGCTCGGC GCAGCTGTGC AATGTCCCAA CAAGGAGCTA 
TTTATTGCTC GCATCAAGGA GCTGGACTTA GAAACGCAAC ACGCTATTGT TGGACTGATC 
AAACAAGTGA CCGACAGCCA TAGTTTGGTG CTCACCGAGG ACTCCTTGGA GCGACTTACG 
CCGCAGAGCA TGTACACGCA CATTTTGCGC CTGACCAAGG AGCGGGACGT CATGTATCTC 
AAGTGGATCG ATTTGGCGTG CGTAGAGACC GAAATGACAG CCAGCGATAA TTTGGTGGAA 
TGTGGCCAAG GTGTCAGCGT TACACGTTCC CCTTCGAACG GAACTGCCAC CTCGACACCT 
TCATCTTCTT CCAATTCGGA AAGCAATCAC CTTGCCGTAG AATGTGCCGA TCTGCGTTCG 
AAGAACCGCA AGCTGCGTCA GGAACTCGAG GAAAAGTCCG AGAATCTTTT GGAGCTACGT 
GAGGAGTTGG ATGACAAGAA GGCTCGCTTT GACAAGCTGC GCCAGGAAAG CCAAGAATGG 
TTTACGGAGG CCAAGCGGGC ATCTGCATAT CGTGACGAGG TGGACATCCT CCGGGAACGG 
GCTGAAAGAG CTGATCGCCT GGAGGTGGAA GTTCAGAAGT ACCGCGAGAA ACTCGGAGAC 
TCCGACTTTT ACAAATCCCG CGTTGAGGAA CTGCGGGAGG ATAACCGTGT GCTGCTTGAA 
TCCAAGGAAA TGCTGGAGGA GCAGTTGCAG CGTTACCGAA AAAGGTCTGA GCATGCCATC 
TCTCTGGAAT CCGAGATTAT TAAATACAAA CAGAAGATAA ACGACATGGC TCTCGAACGG 
GACGTGGATC GCTCCAAGCT AGAAGAGTTG CTGGAGGAGA ACTCGCAACT GCAACTGGTA 
GCCAGGAATC TCAACTCCAC GATGGATTTG GATAAATCCT TTTCCGAAAA CGAAGACGAT 
TGCAACTCAG GCGACAATAG TCTTTCGGAA CAGCTAACGA ACAATGCTCA GACCCGTGCA 
CTCAAGCTCG AGCTGGAGAA CCGTCGTTTG ACCGCCGCTC TGGAACAATT AAAAGAGAGT 
AGCTTCCACG AATCCACCAG CAAGATGCTA GAACTGGAAA AGGAGAAGAA AAAGCTGTCT 
CTAAAAATCG AACAGATGCA AGAAAACATC AATCGTCTGA CTCAGCAGAA TGTCGAACTG 
GAAGGGGTCT TTAAAAATGC CCTGGAGGAG AACAAAAAGT TACAGGATGC CGTGGATAAT 
CGCCAAAAAA GCTACGATCG ACAGAGCCTG GAACGCGAAG CTGACCGTCA AAAGCTTTCT 
GATGCCGAGC AGCATGTCGA AACCTTAAAC AAAGAAAAGC AACGTATCCA AACCCTGAAC 
GAAAGCATTC AGCGAAGAGC CGACGATCTT GAACGGTTGG CGGAGAGCAA GACAAAGGAA 
CTAGAGCAAT ACCTGGAAAA ATCAAGGCAA TACGAGCTTA CTAAACAGAA GCTCTATGAG 
ATCGAAGCAA GGGTCTCCAC GTACGAACGC GAAAACGCCA GCCTGCTCAA GGAAGTGTCC 
AAACTCAAGG AGGGCAGCGA GCAAAAGTCT GTGCAGCTGG ATGACAGTAT TAATCGGCTC 
GATGTCCAGA GCAAAGAACT ACAAAAGCTG GGCAAAGCAC TTGAGGATTC GGAGCAAGTG 
CATCAAAAGC TTGTGGAACT GGAGAAGCAA AACCAAGAGC TAGCTTCACA GCGCATCATT 
GATCAAGAGA TGATAAGTAC TCTTCGAAAC GACTTGGTGA CTGGTACTCT CGTAACGAAG 
AAAGTGCGAA ACAACTTGGA GAAACTAGGA TTGGCGGACG AAGAGCCCGG TGAGCTCAAT 
GTGGAGCATG TGGTGGAGAA GTTGGTGCGC AACCCGGAGA CTTTTAAAAC GGTTCGCGAG 
ATAATGCTAA ACGTGACCCG AGAGCAGCTG GAGGAGGAGG AACGAGAAGG TGGCGTCAAG 
TCCGACATGT GTGTGCTATG CCACCGTCAG GAGATCTTTA CGGTGGAAAA GAACATTGAA 
CTCGCGGCTA CTCCAGCTCC TGCACCAGCG CAACCATCTT CGCAGGAACT TCGCTTCGAA 
CACAAGGTAC GTCTAAGTCC AGCACGAGAA TCAGCAGAGC TGACCCGTAT TAAGGATTCC 
AACACTCAGC TTCAGACTGA GAATGCTCGG CTCAGCGTGG ACGTGGCTGC GCTGGGCTCG 
CAAATAACTT CGCTGAACAC CCAGCATGTA GCCCTACAGC TGGCAAACTC CCAGTTGGCT 
GCCGAAAAAG ATTCGCTGCT CAAAGAGATC GATTCACTGC AGCAGGAGCA CAAGCATGCT 
TTGCAGGATC AGGTTACGTT GCAATGCCTG CATGATCAAC TCTCCGCGGA GTACGAGTCT 
CTGAACAAGG ACAAGGAGCA GCTAAAGGCT GCAGTGCGGG ACTTGCGTCA GGAGCTGCGA 
GATACCCGTG AACAGCAGTC GGCATTGGAG CAACGCATTG AGGAGTTGAC TATTCAGAAC 
AGCAACATGA AAACATGCAG CGAGGATCTG TCAATCCTGC GTACGGAGCA CTCCAAACTG 
ACAGACGACT TCCGCAATCT GTTCGCCACC AGCGATCGCT TCAAGAACGA ATACAAAAAC 
ATCCAGGAGC AGTACAAGAT GGTACGCATG GAGCACTCCA GCCTTAAGCT ACAAAACACA 
GAACTCTCCG GCGAGCTAAA TGCTAAGAGC GATCAGGTCC GTTGCCTACA GATGGAGTAT 
AGCAAGGTTC AGCAGCGTTG TGAGATGTTG ATACAAAACA ATGCTGAACT GGACTCGGAG 
CGCAAGGCTC TGATGGACAA CGTGTCCCAA TTGCTCTCTC AATATCAGGA ACTATTGGCC 
ATATCCCTGG AGGACAAAAA GCACTTCCAT GAGGAGGAGA AGAACTACAC CGAACGGGTG 
CACAGCCTCA AGCGGCAGAA GGAAAAACTG GAGGAGAAAA TTATGGAGCA TTACAAGAAG 
TCAGAAACCA CAGTGCACAA GAAAAAACCA TTTGCCAGCA TGCTGGTTAG AAGAGTGAAG 
AAGGCTAGCT CGGATCTAAT GAACAAGGTG CCCAGTCGAA ACCGTCGATC TTGGGTAGAT 
GACGCCCGTA CCAATTCCCA GTTCGTGATC GGTTCCGAGT CAGGCGGCAA TGAGTCGGAT 
AATAGCAATG AGGAGCCACT GTCCATTGCC TCCGACACGC ACCTGCTCCA GAGGAATGTC 
CCGCTCCGCC AGAGTCTGCA GCGGGATTTG CTGGATAACT CGATCCAACG CGGCGGTGCC 
GTGCGCAGTA GCCTGCAGGC TCAGAAACCA GTCTTGAAGC GCCAGACGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261359.1
CDS	424..4134
Translation	MAGTATATPMEIDEFVNSSIISWLESCLPRAELLAGYTSLLDGHIIHSVWLQIDPEPQNNPSELNDLNGKSLSIARAKNFECVVRNLKSFFEEELGQTILVLPDAFTLGHHPESKNGLEQMKTLLTLLLGAAVQCPNKELFIARIKELDLETQHAIVGLIKQVTDSHSLVLTEDSLERLTPQSMYTHILRLTKERDVMYLKWIDLACVETEMTASDNLVECGQGVSVTRSPSNGTATSTPSSSSNSESNHLAVECADLRSKNRKLRQELEEKSENLLELREELDDKKARFDKLRQESQEWFTEAKRASAYRDEVDILRERAERADRLEVEVQKYREKLGDSDFYKSRVEELREDNRVLLESKEMLEEQLQRYRKRSEHAISLESEIIKYKQKINDMALERDVDRSKLEELLEENSQLQLVARNLNSTMDLDKSFSENEDDCNSGDNSLSEQLTNNAQTRALKLELENRRLTAALEQLKESSFHESTSKMLELEKEKKKLSLKIEQMQENINRLTQQNVELEGVFKNALEENKKLQDAVDNRQKSYDRQSLEREADRQKLSDAEQHVETLNKEKQRIQTLNESIQRRADDLERLAESKTKELEQYLEKSRQYELTKQKLYEIEARVSTYERENASLLKEVSKLKEGSEQKSVQLDDSINRLDVQSKELQKLGKALEDSEQVHQKLVELEKQNQELASQRIIDQEMISTLRNDLVTGTLVTKKVRNNLEKLGLADEEPGELNVEHVVEKLVRNPETFKTVREIMLNVTREQLEEEEREGGVKSDMCVLCHRQEIFTVEKNIELAATPAPAPAQPSSQELRFEHKVRLSPARESAELTRIKDSNTQLQTENARLSVDVAALGSQITSLNTQHVALQLANSQLAAEKDSLLKEIDSLQQEHKHALQDQVTLQCLHDQLSAEYESLNKDKEQLKAAVRDLRQELRDTREQQSALEQRIEELTIQNSNMKTCSEDLSILRTEHSKLTDDFRNLFATSDRFKNEYKNIQEQYKMVRMEHSSLKLQNTELSGELNAKSDQVRCLQMEYSKVQQRCEMLIQNNAELDSERKALMDNVSQLLSQYQELLAISLEDKKHFHEEEKNYTERVHSLKRQKEKLEEKIMEHYKKSETTVHKKKPFASMLVRRVKKASSDLMNKVPSRNRRSWVDDARTNSQFVIGSESGGNESDNSNEEPLSIASDTHLLQRNVPLRQSLQRDLLDNSIQRGGAVRSSLQAQKPVLKRQT

Target ORF information:

RefSeq Version	NM_001274430.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster girdin, transcript variant D (Girdin), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274430.1

ORF Insert Sequence:

ATGGCTGGCA CTGCGACCGC GACGCCAATG GAAATTGATG AGTTTGTTAA TTCGTCCATA 
ATTTCTTGGC TGGAATCATG TCTGCCACGT GCCGAGCTTC TCGCCGGATA CACCTCCCTG 
CTGGATGGCC ACATAATTCA CAGCGTCTGG CTGCAAATCG ACCCCGAGCC GCAAAATAAT 
CCCAGCGAGC TGAACGATCT TAACGGGAAA TCTCTCAGCA TTGCGAGGGC CAAGAACTTC 
GAGTGCGTCG TGAGGAATCT AAAGTCTTTC TTTGAGGAGG AGCTTGGTCA AACCATACTG 
GTCCTACCCG ATGCCTTCAC CCTGGGTCAT CATCCGGAGA GTAAGAACGG GCTCGAACAG 
ATGAAGACCT TGCTGACCCT GTTGCTCGGC GCAGCTGTGC AATGTCCCAA CAAGGAGCTA 
TTTATTGCTC GCATCAAGGA GCTGGACTTA GAAACGCAAC ACGCTATTGT TGGACTGATC 
AAACAAGTGA CCGACAGCCA TAGTTTGGTG CTCACCGAGG ACTCCTTGGA GCGACTTACG 
CCGCAGAGCA TGTACACGCA CATTTTGCGC CTGACCAAGG AGCGGGACGT CATGTATCTC 
AAGTGGATCG ATTTGGCGTG CGTAGAGACC GAAATGACAG CCAGCGATAA TTTGGTGGAA 
TGTGGCCAAG GTGTCAGCGT TACACGTTCC CCTTCGAACG GAACTGCCAC CTCGACACCT 
TCATCTTCTT CCAATTCGGA AAGCAATCAC CTTGCCGTAG AATGTGCCGA TCTGCGTTCG 
AAGAACCGCA AGCTGCGTCA GGAACTCGAG GAAAAGTCCG AGAATCTTTT GGAGCTACGT 
GAGGAGTTGG ATGACAAGAA GGCTCGCTTT GACAAGCTGC GCCAGGAAAG CCAAGAATGG 
TTTACGGAGG CCAAGCGGGC ATCTGCATAT CGTGACGAGG TGGACATCCT CCGGGAACGG 
GCTGAAAGAG CTGATCGCCT GGAGGTGGAA GTTCAGAAGT ACCGCGAGAA ACTCGGAGAC 
TCCGACTTTT ACAAATCCCG CGTTGAGGAA CTGCGGGAGG ATAACCGTGT GCTGCTTGAA 
TCCAAGGAAA TGCTGGAGGA GCAGTTGCAG CGTTACCGAA AAAGGTCTGA GCATGCCATC 
TCTCTGGAAT CCGAGATTAT TAAATACAAA CAGAAGATAA ACGACATGGC TCTCGAACGG 
GACGTGGATC GCTCCAAGCT AGAAGAGTTG CTGGAGGAGA ACTCGCAACT GCAACTGGTA 
GCCAGGAATC TCAACTCCAC GATGGATTTG GATAAATCCT TTTCCGAAAA CGAAGACGAT 
TGCAACTCAG GCGACAATAG TCTTTCGGAA CAGCTAACGA ACAATGCTCA GACCCGTGCA 
CTCAAGCTCG AGCTGGAGAA CCGTCGTTTG ACCGCCGCTC TGGAACAATT AAAAGAGAGT 
AGCTTCCACG AATCCACCAG CAAGATGCTA GAACTGGAAA AGGAGAAGAA AAAGCTGTCT 
CTAAAAATCG AACAGATGCA AGAAAACATC AATCGTCTGA CTCAGCAGAA TGTCGAACTG 
GAAGGGGTCT TTAAAAATGC CCTGGAGGAG AACAAAAAGT TACAGGATGC CGTGGATAAT 
CGCCAAAAAA GCTACGATCG ACAGAGCCTG GAACGCGAAG CTGACCGTCA AAAGCTTTCT 
GATGCCGAGC AGCATGTCGA AACCTTAAAC AAAGAAAAGC AACGTATCCA AACCCTGAAC 
GAAAGCATTC AGCGAAGAGC CGACGATCTT GAACGGTTGG CGGAGAGCAA GACAAAGGAA 
CTAGAGCAAT ACCTGGAAAA ATCAAGGCAA TACGAGCTTA CTAAACAGAA GCTCTATGAG 
ATCGAAGCAA GGGTCTCCAC GTACGAACGC GAAAACGCCA GCCTGCTCAA GGAAGTGTCC 
AAACTCAAGG AGGGCAGCGA GCAAAAGTCT GTGCAGCTGG ATGACAGTAT TAATCGGCTC 
GATGTCCAGA GCAAAGAACT ACAAAAGCTG GGCAAAGCAC TTGAGGATTC GGAGCAAGTG 
CATCAAAAGC TTGTGGAACT GGAGAAGCAA AACCAAGAGC TAGCTTCACA GCGCATCATT 
GATCAAGAGA TGATAAGTAC TCTTCGAAAC GACTTGGTGA CTGGTACTCT CGTAACGAAG 
AAAGTGCGAA ACAACTTGGA GAAACTAGGA TTGGCGGACG AAGAGCCCGG TGAGCTCAAT 
GTGGAGCATG TGGTGGAGAA GTTGGTGCGC AACCCGGAGA CTTTTAAAAC GGTTCGCGAG 
ATAATGCTAA ACGTGACCCG AGAGCAGCTG GAGGAGGAGG AACGAGAAGG TGGCGTCAAG 
TCCGACATGT GTGTGCTATG CCACCGTCAG GAGATCTTTA CGGTGGAAAA GAACATTGAA 
CTCGCGGCTA CTCCAGCTCC TGCACCAGCG CAACCATCTT CGCAGGAACT TCGCTTCGAA 
CACAAGGTAC GTCTAAGTCC AGCACGAGAA TCAGCAGAGC TGACCCGTAT TAAGGATTCC 
AACACTCAGC TTCAGACTGA GAATGCTCGG CTCAGCGTGG ACGTGGCTGC GCTGGGCTCG 
CAAATAACTT CGCTGAACAC CCAGCATGTA GCCCTACAGC TGGCAAACTC CCAGTTGGCT 
GCCGAAAAAG ATTCGCTGCT CAAAGAGATC GATTCACTGC AGCAGGAGCA CAAGCATGCT 
TTGCAGGATC AGGTTACGTT GCAATGCCTG CATGATCAAC TCTCCGCGGA GTACGAGTCT 
CTGAACAAGG ACAAGGAGCA GCTAAAGGCT GCAGTGCGGG ACTTGCGTCA GGAGCTGCGA 
GATACCCGTG AACAGCAGTC GGCATTGGAG CAACGCATTG AGGAGTTGAC TATTCAGAAC 
AGCAACATGA AAACATGCAG CGAGGATCTG TCAATCCTGC GTACGGAGCA CTCCAAACTG 
ACAGACGACT TCCGCAATCT GTTCGCCACC AGCGATCGCT TCAAGAACGA ATACAAAAAC 
ATCCAGGAGC AGTACAAGAT GGTACGCATG GAGCACTCCA GCCTTAAGCT ACAAAACACA 
GAACTCTCCG GCGAGCTAAA TGCTAAGAGC GATCAGGTCC GTTGCCTACA GATGGAGTAT 
AGCAAGGTTC AGCAGCGTTG TGAGATGTTG ATACAAAACA ATGCTGAACT GGACTCGGAG 
CGCAAGGCTC TGATGGACAA CGTGTCCCAA TTGCTCTCTC AATATCAGGA ACTATTGGCC 
ATATCCCTGG AGGACAAAAA GCACTTCCAT GAGGAGGAGA AGAACTACAC CGAACGGGTG 
CACAGCCTCA AGCGGCAGAA GGAAAAACTG GAGGAGAAAA TTATGGAGCA TTACAAGAAG 
TCAGAAACCA CAGTGCACAA GAAAAAACCA TTTGCCAGCA TGCTGGTTAG AAGAGTGAAG 
AAGGCTAGCT CGGATCTAAT GAACAAGGTG CCCAGTCGAA ACCGTCGATC TTGGGTAGAT 
GACGCCCGTA CCAATTCCCA GTTCGTGATC GGTTCCGAGT CAGGCGGCAA TGAGTCGGAT 
AATAGCAATG AGGAGCCACT GTCCATTGCC TCCGACACGC ACCTGCTCCA GAGGAATGTC 
CCGCTCCGCC AGAGTCTGCA GCGGGATTTG CTGGATAACT CGATCCAACG CGGCGGTGCC 
GTGCGCAGTA GCCTGCAGGC TCAGAAACCA GTCTTGAAGC GCCAGACGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Girdin ( NM_206262.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995984.1 Drosophila melanogaster girdin, transcript variant B (Girdin), mRNA.

Price & Availability↑

CloneID	OFa20529
Clone ID Related Accession (Same CDS sequence)	NM_206262.3
Accession Version	NM_206262.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	girdin, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_206262.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTGGCA CTGCGACCGC GACGCCAATG GAAATTGATG AGTTTGTTAA TTCGTCCATA 
ATTTCTTGGC TGGAATCATG TCTGCCACGT GCCGAGCTTC TCGCCGGATA CACCTCCCTG 
CTGGATGGCC ACATAATTCA CAGCGTCTGG CTGCAAATCG ACCCCGAGCC GCAAAATAAT 
CCCAGCGAGC TGAACGATCT TAACGGGAAA TCTCTCAGCA TTGCGAGGGC CAAGAACTTC 
GAGTGCGTCG TGAGGAATCT AAAGTCTTTC TTTGAGGAGG AGCTTGGTCA AACCATACTG 
GTCCTACCCG ATGCCTTCAC CCTGGGTCAT CATCCGGAGA GTAAGAACGG GCTCGAACAG 
ATGAAGACCT TGCTGACCCT GTTGCTCGGC GCAGCTGTGC AATGTCCCAA CAAGGAGCTA 
TTTATTGCTC GCATCAAGGA GCTGGACTTA GAAACGCAAC ACGCTATTGT TGGACTGATC 
AAACAAGTGA CCGACAGCCA TAGTTTGGTG CTCACCGAGG ACTCCTTGGA GCGACTTACG 
CCGCAGAGCA TGTACACGCA CATTTTGCGC CTGACCAAGG AGCGGGACGT CATGTATCTC 
AAGTGGATCG ATTTGGCGTG CGTAGAGACC GAAATGACAG CCAGCGATAA TTTGGTGGAA 
TGTGGCCAAG GTGTCAGCGT TACACGTTCC CCTTCGAACG GAACTGCCAC CTCGACACCT 
TCATCTTCTT CCAATTCGGA AAGCAATCAC CTTGCCGTAG AATGTGCCGA TCTGCGTTCG 
AAGAACCGCA AGCTGCGTCA GGAACTCGAG GAAAAGTCCG AGAATCTTTT GGAGCTACGT 
GAGGAGTTGG ATGACAAGAA GGCTCGCTTT GACAAGCTGC GCCAGGAAAG CCAAGAATGG 
TTTACGGAGG CCAAGCGGGC ATCTGCATAT CGTGACGAGG TGGACATCCT CCGGGAACGG 
GCTGAAAGAG CTGATCGCCT GGAGGTGGAA GTTCAGAAGT ACCGCGAGAA ACTCGGAGAC 
TCCGACTTTT ACAAATCCCG CGTTGAGGAA CTGCGGGAGG ATAACCGTGT GCTGCTTGAA 
TCCAAGGAAA TGCTGGAGGA GCAGTTGCAG CGTTACCGAA AAAGGTCTGA GCATGCCATC 
TCTCTGGAAT CCGAGATTAT TAAATACAAA CAGAAGATAA ACGACATGGC TCTCGAACGG 
GACGTGGATC GCTCCAAGCT AGAAGAGTTG CTGGAGGAGA ACTCGCAACT GCAACTGGTA 
GCCAGGAATC TCAACTCCAC GATGGATTTG GATAAATCCT TTTCCGAAAA CGAAGACGAT 
TGCAACTCAG GCGACAATAG TCTTTCGGAA CAGCTAACGA ACAATGCTCA GACCCGTGCA 
CTCAAGCTCG AGCTGGAGAA CCGTCGTTTG ACCGCCGCTC TGGAACAATT AAAAGAGAGT 
AGCTTCCACG AATCCACCAG CAAGATGCTA GAACTGGAAA AGGAGAAGAA AAAGCTGTCT 
CTAAAAATCG AACAGATGCA AGAAAACATC AATCGTCTGA CTCAGCAGAA TGTCGAACTG 
GAAGGGGTCT TTAAAAATGC CCTGGAGGAG AACAAAAAGT TACAGGATGC CGTGGATAAT 
CGCCAAAAAA GCTACGATCG ACAGAGCCTG GAACGCGAAG CTGACCGTCA AAAGCTTTCT 
GATGCCGAGC AGCATGTCGA AACCTTAAAC AAAGAAAAGC AACGTATCCA AACCCTGAAC 
GAAAGCATTC AGCGAAGAGC CGACGATCTT GAACGGTTGG CGGAGAGCAA GACAAAGGAA 
CTAGAGCAAT ACCTGGAAAA ATCAAGGCAA TACGAGCTTA CTAAACAGAA GCTCTATGAG 
ATCGAAGCAA GGGTCTCCAC GTACGAACGC GAAAACGCCA GCCTGCTCAA GGAAGTGTCC 
AAACTCAAGG AGGGCAGCGA GCAAAAGTCT GTGCAGCTGG ATGACAGTAT TAATCGGCTC 
GATGTCCAGA GCAAAGAACT ACAAAAGCTG GGCAAAGCAC TTGAGGATTC GGAGCAAGTG 
CATCAAAAGC TTGTGGAACT GGAGAAGCAA AACCAAGAGC TAGCTTCACA GCGCATCATT 
GATCAAGAGA TGATAAGTAC TCTTCGAAAC GACTTGGTGA CTGGTACTCT CGTAACGAAG 
AAAGTGCGAA ACAACTTGGA GAAACTAGGA TTGGCGGACG AAGAGCCCGG TGAGCTCAAT 
GTGGAGCATG TGGTGGAGAA GTTGGTGCGC AACCCGGAGA CTTTTAAAAC GGTTCGCGAG 
ATAATGCTAA ACGTGACCCG AGAGCAGCTG GAGGAGGAGG AACGAGAAGG TGGCGTCAAG 
TCCGACATGT GTGTGCTATG CCACCGTCAG GAGATCTTTA CGGTGGAAAA GAACATTGAA 
CTCGCGGCTA CTCCAGCTCC TGCACCAGCG CAACCATCTT CGCAGGAACT TCGCTTCGAA 
CACAAGGTAC GTCTAAGTCC AGCACGAGAA TCAGCAGAGC TGACCCGTAT TAAGGATTCC 
AACACTCAGC TTCAGACTGA GAATGCTCGG CTCAGCGTGG ACGTGGCTGC GCTGGGCTCG 
CAAATAACTT CGCTGAACAC CCAGCATGTA GCCCTACAGC TGGCAAACTC CCAGTTGGCT 
GCCGAAAAAG ATTCGCTGCT CAAAGAGATC GATTCACTGC AGCAGGAGCA CAAGCATGCT 
TTGCAGGATC AGGTTACGTT GCAATGCCTG CATGATCAAC TCTCCGCGGA GTACGAGTCT 
CTGAACAAGG ACAAGGAGCA GCTAAAGGCT GCAGTGCGGG ACTTGCGTCA GGAGCTGCGA 
GATACCCGTG AACAGCAGTC GGCATTGGAG CAACGCATTG AGGAGTTGAC TATTCAGAAC 
AGCAACATGA AAACATGCAG CGAGGATCTG TCAATCCTGC GTACGGAGCA CTCCAAACTG 
ACAGACGACT TCCGCAATCT GTTCGCCACC AGCGATCGCT TCAAGAACGA ATACAAAAAC 
ATCCAGGAGC AGTACAAGAT GGTACGCATG GAGCACTCCA GCCTTAAGCT ACAAAACACA 
GAACTCTCCG GCGAGCTAAA TGCTAAGAGC GATCAGGTCC GTTGCCTACA GATGGAGTAT 
AGCAAGGTTC AGCAGCGTTG TGAGATGTTG ATACAAAACA ATGCTGAACT GGACTCGGAG 
CGCAAGGCTC TGATGGACAA CGTGTCCCAA TTGCTCTCTC AATATCAGGA ACTATTGGCC 
ATATCCCTGG AGGACAAAAA GCACTTCCAT GAGGAGGAGA AGAACTACAC CGAACGGGTG 
CACAGCCTCA AGCGGCAGAA GGAAAAACTG GAGGAGAAAA TTATGGAGCA TTACAAGAAG 
TCAGAAACCA CAGTGCACAA GAAAAAACCA TTTGCCAGCA TGCTGGTTAG AAGAGTGAAG 
AAGGCTAGCT CGGATCTAAT GAACAAGGTG CCCAGTCGAA ACCGTCGATC TTGGGTAGAT 
GACGCCCGTA CCAATTCCCA GTTCGTGATC GGTTCCGAGT CAGGCGGCAA TGAGTCGGAT 
AATAGCAATG AGGAGCCACT GTCCATTGCC TCCGACACGC ACCTGCTCCA GAGGAATGTC 
CCGCTCCGCC AGAGTCTGCA GCGGGATTTG CTGGATAACT CGATCCAACG CGGCGGTGCC 
GTGCGCAGTA GCCTGCAGGC TCAGAAACTC TTGAAGCGCC AGACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995984.1
CDS	424..4131
Translation	MAGTATATPMEIDEFVNSSIISWLESCLPRAELLAGYTSLLDGHIIHSVWLQIDPEPQNNPSELNDLNGKSLSIARAKNFECVVRNLKSFFEEELGQTILVLPDAFTLGHHPESKNGLEQMKTLLTLLLGAAVQCPNKELFIARIKELDLETQHAIVGLIKQVTDSHSLVLTEDSLERLTPQSMYTHILRLTKERDVMYLKWIDLACVETEMTASDNLVECGQGVSVTRSPSNGTATSTPSSSSNSESNHLAVECADLRSKNRKLRQELEEKSENLLELREELDDKKARFDKLRQESQEWFTEAKRASAYRDEVDILRERAERADRLEVEVQKYREKLGDSDFYKSRVEELREDNRVLLESKEMLEEQLQRYRKRSEHAISLESEIIKYKQKINDMALERDVDRSKLEELLEENSQLQLVARNLNSTMDLDKSFSENEDDCNSGDNSLSEQLTNNAQTRALKLELENRRLTAALEQLKESSFHESTSKMLELEKEKKKLSLKIEQMQENINRLTQQNVELEGVFKNALEENKKLQDAVDNRQKSYDRQSLEREADRQKLSDAEQHVETLNKEKQRIQTLNESIQRRADDLERLAESKTKELEQYLEKSRQYELTKQKLYEIEARVSTYERENASLLKEVSKLKEGSEQKSVQLDDSINRLDVQSKELQKLGKALEDSEQVHQKLVELEKQNQELASQRIIDQEMISTLRNDLVTGTLVTKKVRNNLEKLGLADEEPGELNVEHVVEKLVRNPETFKTVREIMLNVTREQLEEEEREGGVKSDMCVLCHRQEIFTVEKNIELAATPAPAPAQPSSQELRFEHKVRLSPARESAELTRIKDSNTQLQTENARLSVDVAALGSQITSLNTQHVALQLANSQLAAEKDSLLKEIDSLQQEHKHALQDQVTLQCLHDQLSAEYESLNKDKEQLKAAVRDLRQELRDTREQQSALEQRIEELTIQNSNMKTCSEDLSILRTEHSKLTDDFRNLFATSDRFKNEYKNIQEQYKMVRMEHSSLKLQNTELSGELNAKSDQVRCLQMEYSKVQQRCEMLIQNNAELDSERKALMDNVSQLLSQYQELLAISLEDKKHFHEEEKNYTERVHSLKRQKEKLEEKIMEHYKKSETTVHKKKPFASMLVRRVKKASSDLMNKVPSRNRRSWVDDARTNSQFVIGSESGGNESDNSNEEPLSIASDTHLLQRNVPLRQSLQRDLLDNSIQRGGAVRSSLQAQKLLKRQT

Target ORF information:

RefSeq Version	NM_206262.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster girdin, transcript variant B (Girdin), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206262.3

ORF Insert Sequence:

ATGGCTGGCA CTGCGACCGC GACGCCAATG GAAATTGATG AGTTTGTTAA TTCGTCCATA 
ATTTCTTGGC TGGAATCATG TCTGCCACGT GCCGAGCTTC TCGCCGGATA CACCTCCCTG 
CTGGATGGCC ACATAATTCA CAGCGTCTGG CTGCAAATCG ACCCCGAGCC GCAAAATAAT 
CCCAGCGAGC TGAACGATCT TAACGGGAAA TCTCTCAGCA TTGCGAGGGC CAAGAACTTC 
GAGTGCGTCG TGAGGAATCT AAAGTCTTTC TTTGAGGAGG AGCTTGGTCA AACCATACTG 
GTCCTACCCG ATGCCTTCAC CCTGGGTCAT CATCCGGAGA GTAAGAACGG GCTCGAACAG 
ATGAAGACCT TGCTGACCCT GTTGCTCGGC GCAGCTGTGC AATGTCCCAA CAAGGAGCTA 
TTTATTGCTC GCATCAAGGA GCTGGACTTA GAAACGCAAC ACGCTATTGT TGGACTGATC 
AAACAAGTGA CCGACAGCCA TAGTTTGGTG CTCACCGAGG ACTCCTTGGA GCGACTTACG 
CCGCAGAGCA TGTACACGCA CATTTTGCGC CTGACCAAGG AGCGGGACGT CATGTATCTC 
AAGTGGATCG ATTTGGCGTG CGTAGAGACC GAAATGACAG CCAGCGATAA TTTGGTGGAA 
TGTGGCCAAG GTGTCAGCGT TACACGTTCC CCTTCGAACG GAACTGCCAC CTCGACACCT 
TCATCTTCTT CCAATTCGGA AAGCAATCAC CTTGCCGTAG AATGTGCCGA TCTGCGTTCG 
AAGAACCGCA AGCTGCGTCA GGAACTCGAG GAAAAGTCCG AGAATCTTTT GGAGCTACGT 
GAGGAGTTGG ATGACAAGAA GGCTCGCTTT GACAAGCTGC GCCAGGAAAG CCAAGAATGG 
TTTACGGAGG CCAAGCGGGC ATCTGCATAT CGTGACGAGG TGGACATCCT CCGGGAACGG 
GCTGAAAGAG CTGATCGCCT GGAGGTGGAA GTTCAGAAGT ACCGCGAGAA ACTCGGAGAC 
TCCGACTTTT ACAAATCCCG CGTTGAGGAA CTGCGGGAGG ATAACCGTGT GCTGCTTGAA 
TCCAAGGAAA TGCTGGAGGA GCAGTTGCAG CGTTACCGAA AAAGGTCTGA GCATGCCATC 
TCTCTGGAAT CCGAGATTAT TAAATACAAA CAGAAGATAA ACGACATGGC TCTCGAACGG 
GACGTGGATC GCTCCAAGCT AGAAGAGTTG CTGGAGGAGA ACTCGCAACT GCAACTGGTA 
GCCAGGAATC TCAACTCCAC GATGGATTTG GATAAATCCT TTTCCGAAAA CGAAGACGAT 
TGCAACTCAG GCGACAATAG TCTTTCGGAA CAGCTAACGA ACAATGCTCA GACCCGTGCA 
CTCAAGCTCG AGCTGGAGAA CCGTCGTTTG ACCGCCGCTC TGGAACAATT AAAAGAGAGT 
AGCTTCCACG AATCCACCAG CAAGATGCTA GAACTGGAAA AGGAGAAGAA AAAGCTGTCT 
CTAAAAATCG AACAGATGCA AGAAAACATC AATCGTCTGA CTCAGCAGAA TGTCGAACTG 
GAAGGGGTCT TTAAAAATGC CCTGGAGGAG AACAAAAAGT TACAGGATGC CGTGGATAAT 
CGCCAAAAAA GCTACGATCG ACAGAGCCTG GAACGCGAAG CTGACCGTCA AAAGCTTTCT 
GATGCCGAGC AGCATGTCGA AACCTTAAAC AAAGAAAAGC AACGTATCCA AACCCTGAAC 
GAAAGCATTC AGCGAAGAGC CGACGATCTT GAACGGTTGG CGGAGAGCAA GACAAAGGAA 
CTAGAGCAAT ACCTGGAAAA ATCAAGGCAA TACGAGCTTA CTAAACAGAA GCTCTATGAG 
ATCGAAGCAA GGGTCTCCAC GTACGAACGC GAAAACGCCA GCCTGCTCAA GGAAGTGTCC 
AAACTCAAGG AGGGCAGCGA GCAAAAGTCT GTGCAGCTGG ATGACAGTAT TAATCGGCTC 
GATGTCCAGA GCAAAGAACT ACAAAAGCTG GGCAAAGCAC TTGAGGATTC GGAGCAAGTG 
CATCAAAAGC TTGTGGAACT GGAGAAGCAA AACCAAGAGC TAGCTTCACA GCGCATCATT 
GATCAAGAGA TGATAAGTAC TCTTCGAAAC GACTTGGTGA CTGGTACTCT CGTAACGAAG 
AAAGTGCGAA ACAACTTGGA GAAACTAGGA TTGGCGGACG AAGAGCCCGG TGAGCTCAAT 
GTGGAGCATG TGGTGGAGAA GTTGGTGCGC AACCCGGAGA CTTTTAAAAC GGTTCGCGAG 
ATAATGCTAA ACGTGACCCG AGAGCAGCTG GAGGAGGAGG AACGAGAAGG TGGCGTCAAG 
TCCGACATGT GTGTGCTATG CCACCGTCAG GAGATCTTTA CGGTGGAAAA GAACATTGAA 
CTCGCGGCTA CTCCAGCTCC TGCACCAGCG CAACCATCTT CGCAGGAACT TCGCTTCGAA 
CACAAGGTAC GTCTAAGTCC AGCACGAGAA TCAGCAGAGC TGACCCGTAT TAAGGATTCC 
AACACTCAGC TTCAGACTGA GAATGCTCGG CTCAGCGTGG ACGTGGCTGC GCTGGGCTCG 
CAAATAACTT CGCTGAACAC CCAGCATGTA GCCCTACAGC TGGCAAACTC CCAGTTGGCT 
GCCGAAAAAG ATTCGCTGCT CAAAGAGATC GATTCACTGC AGCAGGAGCA CAAGCATGCT 
TTGCAGGATC AGGTTACGTT GCAATGCCTG CATGATCAAC TCTCCGCGGA GTACGAGTCT 
CTGAACAAGG ACAAGGAGCA GCTAAAGGCT GCAGTGCGGG ACTTGCGTCA GGAGCTGCGA 
GATACCCGTG AACAGCAGTC GGCATTGGAG CAACGCATTG AGGAGTTGAC TATTCAGAAC 
AGCAACATGA AAACATGCAG CGAGGATCTG TCAATCCTGC GTACGGAGCA CTCCAAACTG 
ACAGACGACT TCCGCAATCT GTTCGCCACC AGCGATCGCT TCAAGAACGA ATACAAAAAC 
ATCCAGGAGC AGTACAAGAT GGTACGCATG GAGCACTCCA GCCTTAAGCT ACAAAACACA 
GAACTCTCCG GCGAGCTAAA TGCTAAGAGC GATCAGGTCC GTTGCCTACA GATGGAGTAT 
AGCAAGGTTC AGCAGCGTTG TGAGATGTTG ATACAAAACA ATGCTGAACT GGACTCGGAG 
CGCAAGGCTC TGATGGACAA CGTGTCCCAA TTGCTCTCTC AATATCAGGA ACTATTGGCC 
ATATCCCTGG AGGACAAAAA GCACTTCCAT GAGGAGGAGA AGAACTACAC CGAACGGGTG 
CACAGCCTCA AGCGGCAGAA GGAAAAACTG GAGGAGAAAA TTATGGAGCA TTACAAGAAG 
TCAGAAACCA CAGTGCACAA GAAAAAACCA TTTGCCAGCA TGCTGGTTAG AAGAGTGAAG 
AAGGCTAGCT CGGATCTAAT GAACAAGGTG CCCAGTCGAA ACCGTCGATC TTGGGTAGAT 
GACGCCCGTA CCAATTCCCA GTTCGTGATC GGTTCCGAGT CAGGCGGCAA TGAGTCGGAT 
AATAGCAATG AGGAGCCACT GTCCATTGCC TCCGACACGC ACCTGCTCCA GAGGAATGTC 
CCGCTCCGCC AGAGTCTGCA GCGGGATTTG CTGGATAACT CGATCCAACG CGGCGGTGCC 
GTGCGCAGTA GCCTGCAGGC TCAGAAACTC TTGAAGCGCC AGACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Girdin ( NM_001274429.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261358.1 Drosophila melanogaster girdin, transcript variant C (Girdin), mRNA.

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CloneID	OFa17352
Clone ID Related Accession (Same CDS sequence)	NM_001274429.1
Accession Version	NM_001274429.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	girdin, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTGGCA CTGCGACCGC GACGCCAATG GAAATTGATG AGTTTGTTAA TTCGTCCATA 
ATTTCTTGGC TGGAATCATG TCTGCCACGT GCCGAGCTTC TCGCCGGATA CACCTCCCTG 
CTGGATGGCC ACATAATTCA CAGCGTCTGG CTGCAAATCG ACCCCGAGCC GCAAAATAAT 
CCCAGCGAGC TGAACGATCT TAACGGGAAA TCTCTCAGCA TTGCGAGGGC CAAGAACTTC 
GAGTGCGTCG TGAGGAATCT AAAGTCTTTC TTTGAGGAGG AGCTTGGTCA AACCATACTG 
GTCCTACCCG ATGCCTTCAC CCTGGGTCAT CATCCGGAGA GTAAGAACGG GCTCGAACAG 
ATGAAGACCT TGCTGACCCT GTTGCTCGGC GCAGCTGTGC AATGTCCCAA CAAGGAGCTA 
TTTATTGCTC GCATCAAGGA GCTGGACTTA GAAACGCAAC ACGCTATTGT TGGACTGATC 
AAACAAGTGA CCGACAGCCA TAGTTTGGTG CTCACCGAGG ACTCCTTGGA GCGACTTACG 
CCGCAGAGCA TGTACACGCA CATTTTGCGC CTGACCAAGG AGCGGGACGT CATGTATCTC 
AAGTGGATCG ATTTGGCGTG CGTAGAGACC GAAATGACAG CCAGCGATAA TTTGGTGGAA 
TGTGGCCAAG GTGTCAGCGT TACACGTTCC CCTTCGAACG GAACTGCCAC CTCGACACCT 
TCATCTTCTT CCAATTCGGA AAGCAATCAC CTTGCCGTAG AATGTGCCGA TCTGCGTTCG 
AAGAACCGCA AGCTGCGTCA GGAACTCGAG GAAAAGTCCG AGAATCTTTT GGAGCTACGT 
GAGGAGTTGG ATGACAAGAA GGCTCGCTTT GACAAGCTGC GCCAGGAAAG CCAAGAATGG 
TTTACGGAGG CCAAGCGGGC ATCTGCATAT CGTGACGAGG TGGACATCCT CCGGGAACGG 
GCTGAAAGAG CTGATCGCCT GGAGGTGGAA GTTCAGAAGT ACCGCGAGAA ACTCGGAGAC 
TCCGACTTTT ACAAATCCCG CGTTGAGGAA CTGCGGGAGG ATAACCGTGT GCTGCTTGAA 
TCCAAGGAAA TGCTGGAGGA GCAGTTGCAG CGTTACCGAA AAAGGTCTGA GCATGCCATC 
TCTCTGGAAT CCGAGATTAT TAAATACAAA CAGAAGATAA ACGACATGGC TCTCGAACGG 
GACGTGGATC GCTCCAAGCT AGAAGAGTTG CTGGAGGAGA ACTCGCAACT GCAACTGGTA 
GCCAGGAATC TCAACTCCAC GATGGATTTG GATAAATCCT TTTCCGAAAA CGAAGACGAT 
TGCAACTCAG GCGACAATAG TCTTTCGGAA CAGCTAACGA ACAATGCTCA GACCCGTGCA 
CTCAAGCTCG AGCTGGAGAA CCGTCGTTTG ACCGCCGCTC TGGAACAATT AAAAGAGAGT 
AGCTTCCACG AATCCACCAG CAAGATGCTA GAACTGGAAA AGGAGAAGAA AAAGCTGTCT 
CTAAAAATCG AACAGATGCA AGAAAACATC AATCGTCTGA CTCAGCAGAA TGTCGAACTG 
GAAGGGGTCT TTAAAAATGC CCTGGAGGAG AACAAAAAGT TACAGGATGC CGTGGATAAT 
CGCCAAAAAA GCTACGATCG ACAGAGCCTG GAACGCGAAG CTGACCGTCA AAAGCTTTCT 
GATGCCGAGC AGCATGTCGA AACCTTAAAC AAAGAAAAGC AACGTATCCA AACCCTGAAC 
GAAAGCATTC AGCGAAGAGC CGACGATCTT GAACGGTTGG CGGAGAGCAA GACAAAGGAA 
CTAGAGCAAT ACCTGGAAAA ATCAAGGCAA TACGAGCTTA CTAAACAGAA GCTCTATGAG 
ATCGAAGCAA GGGTCTCCAC GTACGAACGC GAAAACGCCA GCCTGCTCAA GGAAGTGTCC 
AAACTCAAGG AGGGCAGCGA GCAAAAGTCT GTGCAGCTGG ATGACAGTAT TAATCGGCTC 
GATGTCCAGA GCAAAGAACT ACAAAAGCTG GGCAAAGCAC TTGAGGATTC GGAGCAAGTG 
CATCAAAAGC TTGTGGAACT GGAGAAGCAA AACCAAGAGC TAGCTTCACA GCGCATCATT 
GATCAAGAGA TGATAAGTAC TCTTCGAAAC GACTTGGTGA CTGGTACTCT CGTAACGAAG 
AAAGTGCGAA ACAACTTGGA GAAACTAGGA TTGGCGGACG AAGAGCCCGG TGAGCTCAAT 
GTGGAGCATG TGGTGGAGAA GTTGGTGCGC AACCCGGAGA CTTTTAAAAC GGTTCGCGAG 
ATAATGCTAA ACGTGACCCG AGAGCAGCTG GAGGAGGAGG AACGAGAAGG TGGCGTCAAG 
TCCGACATGT GTGTGCTATG CCACCGTCAG GAGATCTTTA CGGTGGAAAA GAACATTGAA 
CTCGCGGCTA CTCCAGCTCC TGCACCAGCG CAACCATCTT CGCAGGAACT TCGCTTCGAA 
CACAAGGTAC GTCTAAGTCC AGCACGAGAA TCAGCAGAGC TGACCCGTAT TAAGGATTCC 
AACACTCAGC TTCAGACTGA GAATGCTCGG CTCAGCGTGG ACGTGGCTGC GCTGGGCTCG 
CAAATAACTT CGCTGAACAC CCAGCATGTA GCCCTACAGC TGGCAAACTC CCAGTTGGCT 
GCCGAAAAAG ATTCGCTGCT CAAAGAGATC GATTCACTGC AGCAGGAGCA CAAGCATGCT 
TTGCAGGATC AGGTTACGTT GCAATGCCTG CATGATCAAC TCTCCGCGGA GTACGAGTCT 
CTGAACAAGG ACAAGGAGCA GCTAAAGGCT GCAGTGCGGG ACTTGCGTCA GGAGCTGCGA 
GATACCCGTG AACAGCAGTC GGCATTGGAG CAACGCATTG AGGAGTTGAC TATTCAGAAC 
AGCAACATGA AAACATGCAG CGAGGATCTG TCAATCCTGC GTACGGAGCA CTCCAAACTG 
ACAGACGACT TCCGCAATCT GTTCGCCACC AGCGATCGCT TCAAGAACGA ATACAAAAAC 
ATCCAGGAGC AGTACAAGAT GGTACGCATG GAGCACTCCA GCCTTAAGCT ACAAAACACA 
GAACTCTCCG GCGAGCTAAA TGCTAAGAGC GATCAGGTCC GTTGCCTACA GATGGAGTAT 
AGCAAGGTTC AGCAGCGTTG TGAGATGTTG ATACAAAACA ATGCTGAACT GGACTCGGAG 
CGCAAGGCTC TGATGGACAA CGTGTCCCAA TTGCTCTCTC AATATCAGGA ACTATTGGCC 
ATATCCCTGG AGGACAAAAA GCACTTCCAT GAGGAGGAGA AGAACTACAC CGAACGGGTG 
CACAGCCTCA AGCGGCAGAA GGAAAAACTG GAGGAGAAAA TTATGGAGCA TTACAAGAAG 
TCAGAAACCA CAGTGCACAA GAAAAAACCA TTTGCCAGCA TGCTGGTTAG AAGAGTGAAG 
AAGGCTAGCT CGGATCTAAT GAACAAGGTG CCCAGTCGAA ACCGTCGATC TTGGGTAGAT 
GACGCCCGTA CCAATTCCCA GTTCGTGATC GGTTCCGAGT CAGGCGGCAA TGAGTCGGAT 
AATAGCAATG AGGAGCCACT GTCCATTGCC TCCGACACGC ACCTGCTCCA GAGGAATGTC 
CCGCTCCGCC AGAGTCTGCA GCGGGATTTG CTGGATAACT CGATCCAACG CGGCGGTGCC 
GTGCGCAGTA GCCTGCAGGC TCAGAAACGT ACGGATTTGA ATAACTCACG TAGAAATAGC 
GTGCACGGTC TTGAAGCGCC AGACGTGACG GGCAGCAGTC TGACTCTCGG AACGGCCGGT 
TCCAGACGCA CCGTTTATCT TATCGATGAG CACCAAAAGC TTCCCGATGG CTCTACTCCC 
GGCGCCACAC AGTCACAGTC TGTTGGCGGC AGTGGCTCCG TCAACGCCGC ACCTCCTACA 
CCCGCAGAAC CTCAGACGCC TCAGAAGAGT ACCGAAAACA ATGCCCCGGT GGGCCCAGCT 
ACGTTTCTCA TGTACAACCG AATAAACACC ACGATCGGAG GAGCCTCGAA CAGCGGGGAC 
CAGAGCCCTC TGCTGCAGGC CAGCGGCAGC GTCTCGGGAA CGACCATGCA GGATGACAAG 
TCGGCAAGGA AGCGGACCGA AGACAAGAGC AATAGCATCT GGTATGAGTA CGGCTGCGTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261358.1
CDS	424..4566
Translation	MAGTATATPMEIDEFVNSSIISWLESCLPRAELLAGYTSLLDGHIIHSVWLQIDPEPQNNPSELNDLNGKSLSIARAKNFECVVRNLKSFFEEELGQTILVLPDAFTLGHHPESKNGLEQMKTLLTLLLGAAVQCPNKELFIARIKELDLETQHAIVGLIKQVTDSHSLVLTEDSLERLTPQSMYTHILRLTKERDVMYLKWIDLACVETEMTASDNLVECGQGVSVTRSPSNGTATSTPSSSSNSESNHLAVECADLRSKNRKLRQELEEKSENLLELREELDDKKARFDKLRQESQEWFTEAKRASAYRDEVDILRERAERADRLEVEVQKYREKLGDSDFYKSRVEELREDNRVLLESKEMLEEQLQRYRKRSEHAISLESEIIKYKQKINDMALERDVDRSKLEELLEENSQLQLVARNLNSTMDLDKSFSENEDDCNSGDNSLSEQLTNNAQTRALKLELENRRLTAALEQLKESSFHESTSKMLELEKEKKKLSLKIEQMQENINRLTQQNVELEGVFKNALEENKKLQDAVDNRQKSYDRQSLEREADRQKLSDAEQHVETLNKEKQRIQTLNESIQRRADDLERLAESKTKELEQYLEKSRQYELTKQKLYEIEARVSTYERENASLLKEVSKLKEGSEQKSVQLDDSINRLDVQSKELQKLGKALEDSEQVHQKLVELEKQNQELASQRIIDQEMISTLRNDLVTGTLVTKKVRNNLEKLGLADEEPGELNVEHVVEKLVRNPETFKTVREIMLNVTREQLEEEEREGGVKSDMCVLCHRQEIFTVEKNIELAATPAPAPAQPSSQELRFEHKVRLSPARESAELTRIKDSNTQLQTENARLSVDVAALGSQITSLNTQHVALQLANSQLAAEKDSLLKEIDSLQQEHKHALQDQVTLQCLHDQLSAEYESLNKDKEQLKAAVRDLRQELRDTREQQSALEQRIEELTIQNSNMKTCSEDLSILRTEHSKLTDDFRNLFATSDRFKNEYKNIQEQYKMVRMEHSSLKLQNTELSGELNAKSDQVRCLQMEYSKVQQRCEMLIQNNAELDSERKALMDNVSQLLSQYQELLAISLEDKKHFHEEEKNYTERVHSLKRQKEKLEEKIMEHYKKSETTVHKKKPFASMLVRRVKKASSDLMNKVPSRNRRSWVDDARTNSQFVIGSESGGNESDNSNEEPLSIASDTHLLQRNVPLRQSLQRDLLDNSIQRGGAVRSSLQAQKRTDLNNSRRNSVHGLEAPDVTGSSLTLGTAGSRRTVYLIDEHQKLPDGSTPGATQSQSVGGSGSVNAAPPTPAEPQTPQKSTENNAPVGPATFLMYNRINTTIGGASNSGDQSPLLQASGSVSGTTMQDDKSARKRTEDKSNSIWYEYGCV

Target ORF information:

RefSeq Version	NM_001274429.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster girdin, transcript variant C (Girdin), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274429.1

ORF Insert Sequence:

ATGGCTGGCA CTGCGACCGC GACGCCAATG GAAATTGATG AGTTTGTTAA TTCGTCCATA 
ATTTCTTGGC TGGAATCATG TCTGCCACGT GCCGAGCTTC TCGCCGGATA CACCTCCCTG 
CTGGATGGCC ACATAATTCA CAGCGTCTGG CTGCAAATCG ACCCCGAGCC GCAAAATAAT 
CCCAGCGAGC TGAACGATCT TAACGGGAAA TCTCTCAGCA TTGCGAGGGC CAAGAACTTC 
GAGTGCGTCG TGAGGAATCT AAAGTCTTTC TTTGAGGAGG AGCTTGGTCA AACCATACTG 
GTCCTACCCG ATGCCTTCAC CCTGGGTCAT CATCCGGAGA GTAAGAACGG GCTCGAACAG 
ATGAAGACCT TGCTGACCCT GTTGCTCGGC GCAGCTGTGC AATGTCCCAA CAAGGAGCTA 
TTTATTGCTC GCATCAAGGA GCTGGACTTA GAAACGCAAC ACGCTATTGT TGGACTGATC 
AAACAAGTGA CCGACAGCCA TAGTTTGGTG CTCACCGAGG ACTCCTTGGA GCGACTTACG 
CCGCAGAGCA TGTACACGCA CATTTTGCGC CTGACCAAGG AGCGGGACGT CATGTATCTC 
AAGTGGATCG ATTTGGCGTG CGTAGAGACC GAAATGACAG CCAGCGATAA TTTGGTGGAA 
TGTGGCCAAG GTGTCAGCGT TACACGTTCC CCTTCGAACG GAACTGCCAC CTCGACACCT 
TCATCTTCTT CCAATTCGGA AAGCAATCAC CTTGCCGTAG AATGTGCCGA TCTGCGTTCG 
AAGAACCGCA AGCTGCGTCA GGAACTCGAG GAAAAGTCCG AGAATCTTTT GGAGCTACGT 
GAGGAGTTGG ATGACAAGAA GGCTCGCTTT GACAAGCTGC GCCAGGAAAG CCAAGAATGG 
TTTACGGAGG CCAAGCGGGC ATCTGCATAT CGTGACGAGG TGGACATCCT CCGGGAACGG 
GCTGAAAGAG CTGATCGCCT GGAGGTGGAA GTTCAGAAGT ACCGCGAGAA ACTCGGAGAC 
TCCGACTTTT ACAAATCCCG CGTTGAGGAA CTGCGGGAGG ATAACCGTGT GCTGCTTGAA 
TCCAAGGAAA TGCTGGAGGA GCAGTTGCAG CGTTACCGAA AAAGGTCTGA GCATGCCATC 
TCTCTGGAAT CCGAGATTAT TAAATACAAA CAGAAGATAA ACGACATGGC TCTCGAACGG 
GACGTGGATC GCTCCAAGCT AGAAGAGTTG CTGGAGGAGA ACTCGCAACT GCAACTGGTA 
GCCAGGAATC TCAACTCCAC GATGGATTTG GATAAATCCT TTTCCGAAAA CGAAGACGAT 
TGCAACTCAG GCGACAATAG TCTTTCGGAA CAGCTAACGA ACAATGCTCA GACCCGTGCA 
CTCAAGCTCG AGCTGGAGAA CCGTCGTTTG ACCGCCGCTC TGGAACAATT AAAAGAGAGT 
AGCTTCCACG AATCCACCAG CAAGATGCTA GAACTGGAAA AGGAGAAGAA AAAGCTGTCT 
CTAAAAATCG AACAGATGCA AGAAAACATC AATCGTCTGA CTCAGCAGAA TGTCGAACTG 
GAAGGGGTCT TTAAAAATGC CCTGGAGGAG AACAAAAAGT TACAGGATGC CGTGGATAAT 
CGCCAAAAAA GCTACGATCG ACAGAGCCTG GAACGCGAAG CTGACCGTCA AAAGCTTTCT 
GATGCCGAGC AGCATGTCGA AACCTTAAAC AAAGAAAAGC AACGTATCCA AACCCTGAAC 
GAAAGCATTC AGCGAAGAGC CGACGATCTT GAACGGTTGG CGGAGAGCAA GACAAAGGAA 
CTAGAGCAAT ACCTGGAAAA ATCAAGGCAA TACGAGCTTA CTAAACAGAA GCTCTATGAG 
ATCGAAGCAA GGGTCTCCAC GTACGAACGC GAAAACGCCA GCCTGCTCAA GGAAGTGTCC 
AAACTCAAGG AGGGCAGCGA GCAAAAGTCT GTGCAGCTGG ATGACAGTAT TAATCGGCTC 
GATGTCCAGA GCAAAGAACT ACAAAAGCTG GGCAAAGCAC TTGAGGATTC GGAGCAAGTG 
CATCAAAAGC TTGTGGAACT GGAGAAGCAA AACCAAGAGC TAGCTTCACA GCGCATCATT 
GATCAAGAGA TGATAAGTAC TCTTCGAAAC GACTTGGTGA CTGGTACTCT CGTAACGAAG 
AAAGTGCGAA ACAACTTGGA GAAACTAGGA TTGGCGGACG AAGAGCCCGG TGAGCTCAAT 
GTGGAGCATG TGGTGGAGAA GTTGGTGCGC AACCCGGAGA CTTTTAAAAC GGTTCGCGAG 
ATAATGCTAA ACGTGACCCG AGAGCAGCTG GAGGAGGAGG AACGAGAAGG TGGCGTCAAG 
TCCGACATGT GTGTGCTATG CCACCGTCAG GAGATCTTTA CGGTGGAAAA GAACATTGAA 
CTCGCGGCTA CTCCAGCTCC TGCACCAGCG CAACCATCTT CGCAGGAACT TCGCTTCGAA 
CACAAGGTAC GTCTAAGTCC AGCACGAGAA TCAGCAGAGC TGACCCGTAT TAAGGATTCC 
AACACTCAGC TTCAGACTGA GAATGCTCGG CTCAGCGTGG ACGTGGCTGC GCTGGGCTCG 
CAAATAACTT CGCTGAACAC CCAGCATGTA GCCCTACAGC TGGCAAACTC CCAGTTGGCT 
GCCGAAAAAG ATTCGCTGCT CAAAGAGATC GATTCACTGC AGCAGGAGCA CAAGCATGCT 
TTGCAGGATC AGGTTACGTT GCAATGCCTG CATGATCAAC TCTCCGCGGA GTACGAGTCT 
CTGAACAAGG ACAAGGAGCA GCTAAAGGCT GCAGTGCGGG ACTTGCGTCA GGAGCTGCGA 
GATACCCGTG AACAGCAGTC GGCATTGGAG CAACGCATTG AGGAGTTGAC TATTCAGAAC 
AGCAACATGA AAACATGCAG CGAGGATCTG TCAATCCTGC GTACGGAGCA CTCCAAACTG 
ACAGACGACT TCCGCAATCT GTTCGCCACC AGCGATCGCT TCAAGAACGA ATACAAAAAC 
ATCCAGGAGC AGTACAAGAT GGTACGCATG GAGCACTCCA GCCTTAAGCT ACAAAACACA 
GAACTCTCCG GCGAGCTAAA TGCTAAGAGC GATCAGGTCC GTTGCCTACA GATGGAGTAT 
AGCAAGGTTC AGCAGCGTTG TGAGATGTTG ATACAAAACA ATGCTGAACT GGACTCGGAG 
CGCAAGGCTC TGATGGACAA CGTGTCCCAA TTGCTCTCTC AATATCAGGA ACTATTGGCC 
ATATCCCTGG AGGACAAAAA GCACTTCCAT GAGGAGGAGA AGAACTACAC CGAACGGGTG 
CACAGCCTCA AGCGGCAGAA GGAAAAACTG GAGGAGAAAA TTATGGAGCA TTACAAGAAG 
TCAGAAACCA CAGTGCACAA GAAAAAACCA TTTGCCAGCA TGCTGGTTAG AAGAGTGAAG 
AAGGCTAGCT CGGATCTAAT GAACAAGGTG CCCAGTCGAA ACCGTCGATC TTGGGTAGAT 
GACGCCCGTA CCAATTCCCA GTTCGTGATC GGTTCCGAGT CAGGCGGCAA TGAGTCGGAT 
AATAGCAATG AGGAGCCACT GTCCATTGCC TCCGACACGC ACCTGCTCCA GAGGAATGTC 
CCGCTCCGCC AGAGTCTGCA GCGGGATTTG CTGGATAACT CGATCCAACG CGGCGGTGCC 
GTGCGCAGTA GCCTGCAGGC TCAGAAACGT ACGGATTTGA ATAACTCACG TAGAAATAGC 
GTGCACGGTC TTGAAGCGCC AGACGTGACG GGCAGCAGTC TGACTCTCGG AACGGCCGGT 
TCCAGACGCA CCGTTTATCT TATCGATGAG CACCAAAAGC TTCCCGATGG CTCTACTCCC 
GGCGCCACAC AGTCACAGTC TGTTGGCGGC AGTGGCTCCG TCAACGCCGC ACCTCCTACA 
CCCGCAGAAC CTCAGACGCC TCAGAAGAGT ACCGAAAACA ATGCCCCGGT GGGCCCAGCT 
ACGTTTCTCA TGTACAACCG AATAAACACC ACGATCGGAG GAGCCTCGAA CAGCGGGGAC 
CAGAGCCCTC TGCTGCAGGC CAGCGGCAGC GTCTCGGGAA CGACCATGCA GGATGACAAG 
TCGGCAAGGA AGCGGACCGA AGACAAGAGC AATAGCATCT GGTATGAGTA CGGCTGCGTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Girdin ( NM_001274431.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261360.1 Drosophila melanogaster girdin, transcript variant E (Girdin), mRNA.

Price & Availability↑

CloneID	OFa17354
Clone ID Related Accession (Same CDS sequence)	NM_001274431.1
Accession Version	NM_001274431.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	girdin, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTGGCA CTGCGACCGC GACGCCAATG GAAATTGATG AGTTTGTTAA TTCGTCCATA 
ATTTCTTGGC TGGAATCATG TCTGCCACGT GCCGAGCTTC TCGCCGGATA CACCTCCCTG 
CTGGATGGCC ACATAATTCA CAGCGTCTGG CTGCAAATCG ACCCCGAGCC GCAAAATAAT 
CCCAGCGAGC TGAACGATCT TAACGGGAAA TCTCTCAGCA TTGCGAGGGC CAAGAACTTC 
GAGTGCGTCG TGAGGAATCT AAAGTCTTTC TTTGAGGAGG AGCTTGGTCA AACCATACTG 
GTCCTACCCG ATGCCTTCAC CCTGGGTCAT CATCCGGAGA GTAAGAACGG GCTCGAACAG 
ATGAAGACCT TGCTGACCCT GTTGCTCGGC GCAGCTGTGC AATGTCCCAA CAAGGAGCTA 
TTTATTGCTC GCATCAAGGA GCTGGACTTA GAAACGCAAC ACGCTATTGT TGGACTGATC 
AAACAAGTGA CCGACAGCCA TAGTTTGGTG CTCACCGAGG ACTCCTTGGA GCGACTTACG 
CCGCAGAGCA TGTACACGCA CATTTTGCGC CTGACCAAGG AGCGGGACGT CATGTATCTC 
AAGTGGATCG ATTTGGCGTG CGTAGAGACC GAAATGACAG CCAGCGATAA TTTGGTGGAA 
TGTGGCCAAG GTGTCAGCGT TACACGTTCC CCTTCGAACG GAACTGCCAC CTCGACACCT 
TCATCTTCTT CCAATTCGGA AAGCAATCAC CTTGCCGTAG AATGTGCCGA TCTGCGTTCG 
AAGAACCGCA AGCTGCGTCA GGAACTCGAG GAAAAGTCCG AGAATCTTTT GGAGCTACGT 
GAGGAGTTGG ATGACAAGAA GGCTCGCTTT GACAAGCTGC GCCAGGAAAG CCAAGAATGG 
TTTACGGAGG CCAAGCGGGC ATCTGCATAT CGTGACGAGG TGGACATCCT CCGGGAACGG 
GCTGAAAGAG CTGATCGCCT GGAGGTGGAA GTTCAGAAGT ACCGCGAGAA ACTCGGAGAC 
TCCGACTTTT ACAAATCCCG CGTTGAGGAA CTGCGGGAGG ATAACCGTGT GCTGCTTGAA 
TCCAAGGAAA TGCTGGAGGA GCAGTTGCAG CGTTACCGAA AAAGGTCTGA GCATGCCATC 
TCTCTGGAAT CCGAGATTAT TAAATACAAA CAGAAGATAA ACGACATGGC TCTCGAACGG 
GACGTGGATC GCTCCAAGCT AGAAGAGTTG CTGGAGGAGA ACTCGCAACT GCAACTGGTA 
GCCAGGAATC TCAACTCCAC GATGGATTTG GATAAATCCT TTTCCGAAAA CGAAGACGAT 
TGCAACTCAG GCGACAATAG TCTTTCGGAA CAGCTAACGA ACAATGCTCA GACCCGTGCA 
CTCAAGCTCG AGCTGGAGAA CCGTCGTTTG ACCGCCGCTC TGGAACAATT AAAAGAGAGT 
AGCTTCCACG AATCCACCAG CAAGATGCTA GAACTGGAAA AGGAGAAGAA AAAGCTGTCT 
CTAAAAATCG AACAGATGCA AGAAAACATC AATCGTCTGA CTCAGCAGAA TGTCGAACTG 
GAAGGGGTCT TTAAAAATGC CCTGGAGGAG AACAAAAAGT TACAGGATGC CGTGGATAAT 
CGCCAAAAAA GCTACGATCG ACAGAGCCTG GAACGCGAAG CTGACCGTCA AAAGCTTTCT 
GATGCCGAGC AGCATGTCGA AACCTTAAAC AAAGAAAAGC AACGTATCCA AACCCTGAAC 
GAAAGCATTC AGCGAAGAGC CGACGATCTT GAACGGTTGG CGGAGAGCAA GACAAAGGAA 
CTAGAGCAAT ACCTGGAAAA ATCAAGGCAA TACGAGCTTA CTAAACAGAA GCTCTATGAG 
ATCGAAGCAA GGGTCTCCAC GTACGAACGC GAAAACGCCA GCCTGCTCAA GGAAGTGTCC 
AAACTCAAGG AGGGCAGCGA GCAAAAGTCT GTGCAGCTGG ATGACAGTAT TAATCGGCTC 
GATGTCCAGA GCAAAGAACT ACAAAAGCTG GGCAAAGCAC TTGAGGATTC GGAGCAAGTG 
CATCAAAAGC TTGTGGAACT GGAGAAGCAA AACCAAGAGC TAGCTTCACA GCGCATCATT 
GATCAAGAGA TGATAAGTAC TCTTCGAAAC GACTTGGTGA CTGGTACTCT CGTAACGAAG 
AAAGTGCGAA ACAACTTGGA GAAACTAGGA TTGGCGGACG AAGAGCCCGG TGAGCTCAAT 
GTGGAGCATG TGGTGGAGAA GTTGGTGCGC AACCCGGAGA CTTTTAAAAC GGTTCGCGAG 
ATAATGCTAA ACGTGACCCG AGAGCAGCTG GAGGAGGAGG AACGAGAAGG TGGCGTCAAG 
TCCGACATGT GTGTGCTATG CCACCGTCAG GAGATCTTTA CGGTGGAAAA GAACATTGAA 
CTCGCGGCTA CTCCAGCTCC TGCACCAGCG CAACCATCTT CGCAGGAACT TCGCTTCGAA 
CACAAGGTAC GTCTAAGTCC AGCACGAGAA TCAGCAGAGC TGACCCGTAT TAAGGATTCC 
AACACTCAGC TTCAGACTGA GAATGCTCGG CTCAGCGTGG ACGTGGCTGC GCTGGGCTCG 
CAAATAACTT CGCTGAACAC CCAGCATGTA GCCCTACAGC TGGCAAACTC CCAGTTGGCT 
GCCGAAAAAG ATTCGCTGCT CAAAGAGATC GATTCACTGC AGCAGGAGCA CAAGCATGCT 
TTGCAGGATC AGGTTACGTT GCAATGCCTG CATGATCAAC TCTCCGCGGA GTACGAGTCT 
CTGAACAAGG ACAAGGAGCA GCTAAAGGCT GCAGTGCGGG ACTTGCGTCA GGAGCTGCGA 
GATACCCGTG AACAGCAGTC GGCATTGGAG CAACGCATTG AGGAGTTGAC TATTCAGAAC 
AGCAACATGA AAACATGCAG CGAGGATCTG TCAATCCTGC GTACGGAGCA CTCCAAACTG 
ACAGACGACT TCCGCAATCT GTTCGCCACC AGCGATCGCT TCAAGAACGA ATACAAAAAC 
ATCCAGGAGC AGTACAAGAT GGTACGCATG GAGCACTCCA GCCTTAAGCT ACAAAACACA 
GAACTCTCCG GCGAGCTAAA TGCTAAGAGC GATCAGGTCC GTTGCCTACA GATGGAGTAT 
AGCAAGGTTC AGCAGCGTTG TGAGATGTTG ATACAAAACA ATGCTGAACT GGACTCGGAG 
CGCAAGGCTC TGATGGACAA CGTGTCCCAA TTGCTCTCTC AATATCAGGA ACTATTGGCC 
ATATCCCTGG AGGACAAAAA GCACTTCCAT GAGGAGGAGA AGAACTACAC CGAACGGGTG 
CACAGCCTCA AGCGGCAGAA GGAAAAACTG GAGGAGAAAA TTATGGAGCA TTACAAGAAG 
TCAGAAACCA CAGTGCACAA GAAAAAACCA TTTGCCAGCA TGCTGGTTAG AAGAGTGAAG 
AAGGCTAGCT CGGATCTAAT GAACAAGGTG CCCAGTCGAA ACCGTCGATC TTGGGTAGAT 
GACGCCCGTA CCAATTCCCA GTTCGTGATC GGTTCCGAGT CAGGCGGCAA TGAGTCGGAT 
AATAGCAATG AGGAGCCACT GTCCATTGCC TCCGACACGC ACCTGCTCCA GAGGAATGTC 
CCGCTCCGCC AGAGTCTGCA GCGCAGTCTT GAAGCGCCAG ACGTGACGGG CAGCAGTCTG 
ACTCTCGGAA CGGCCGGTTC CAGACGCACC GTTTATCTTA TCGATGAGCA CCAAAAGCTT 
CCCGATGGCT CTACTCCCGG CGCCACACAG TCACAGTCTG TTGGCGGCAG TGGCTCCGTC 
AACGCCGCAC CTCCTACACC CGCAGAACCT CAGACGCCTC AGAAGAGTAC CGAAAACAAT 
GCCCCGGTGG GCCCAGCTAC GTTTCTCATG TACAACCGAA TAAACACCAC GATCGGAGGA 
GCCTCGAACA GCGGGGACCA GAGCCCTCTG CTGCAGGCCA GCGGCAGCGT CTCGGGAACG 
ACCATGCAGG ATGACAAGTC GGCAAGGAAG CGGACCGAAG ACAAGAGCAA TAGCATCTGG 
TATGAGTACG GCTGCGTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261360.1
CDS	424..4464
Translation	MAGTATATPMEIDEFVNSSIISWLESCLPRAELLAGYTSLLDGHIIHSVWLQIDPEPQNNPSELNDLNGKSLSIARAKNFECVVRNLKSFFEEELGQTILVLPDAFTLGHHPESKNGLEQMKTLLTLLLGAAVQCPNKELFIARIKELDLETQHAIVGLIKQVTDSHSLVLTEDSLERLTPQSMYTHILRLTKERDVMYLKWIDLACVETEMTASDNLVECGQGVSVTRSPSNGTATSTPSSSSNSESNHLAVECADLRSKNRKLRQELEEKSENLLELREELDDKKARFDKLRQESQEWFTEAKRASAYRDEVDILRERAERADRLEVEVQKYREKLGDSDFYKSRVEELREDNRVLLESKEMLEEQLQRYRKRSEHAISLESEIIKYKQKINDMALERDVDRSKLEELLEENSQLQLVARNLNSTMDLDKSFSENEDDCNSGDNSLSEQLTNNAQTRALKLELENRRLTAALEQLKESSFHESTSKMLELEKEKKKLSLKIEQMQENINRLTQQNVELEGVFKNALEENKKLQDAVDNRQKSYDRQSLEREADRQKLSDAEQHVETLNKEKQRIQTLNESIQRRADDLERLAESKTKELEQYLEKSRQYELTKQKLYEIEARVSTYERENASLLKEVSKLKEGSEQKSVQLDDSINRLDVQSKELQKLGKALEDSEQVHQKLVELEKQNQELASQRIIDQEMISTLRNDLVTGTLVTKKVRNNLEKLGLADEEPGELNVEHVVEKLVRNPETFKTVREIMLNVTREQLEEEEREGGVKSDMCVLCHRQEIFTVEKNIELAATPAPAPAQPSSQELRFEHKVRLSPARESAELTRIKDSNTQLQTENARLSVDVAALGSQITSLNTQHVALQLANSQLAAEKDSLLKEIDSLQQEHKHALQDQVTLQCLHDQLSAEYESLNKDKEQLKAAVRDLRQELRDTREQQSALEQRIEELTIQNSNMKTCSEDLSILRTEHSKLTDDFRNLFATSDRFKNEYKNIQEQYKMVRMEHSSLKLQNTELSGELNAKSDQVRCLQMEYSKVQQRCEMLIQNNAELDSERKALMDNVSQLLSQYQELLAISLEDKKHFHEEEKNYTERVHSLKRQKEKLEEKIMEHYKKSETTVHKKKPFASMLVRRVKKASSDLMNKVPSRNRRSWVDDARTNSQFVIGSESGGNESDNSNEEPLSIASDTHLLQRNVPLRQSLQRSLEAPDVTGSSLTLGTAGSRRTVYLIDEHQKLPDGSTPGATQSQSVGGSGSVNAAPPTPAEPQTPQKSTENNAPVGPATFLMYNRINTTIGGASNSGDQSPLLQASGSVSGTTMQDDKSARKRTEDKSNSIWYEYGCV

Target ORF information:

RefSeq Version	NM_001274431.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster girdin, transcript variant E (Girdin), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274431.1

ORF Insert Sequence:

ATGGCTGGCA CTGCGACCGC GACGCCAATG GAAATTGATG AGTTTGTTAA TTCGTCCATA 
ATTTCTTGGC TGGAATCATG TCTGCCACGT GCCGAGCTTC TCGCCGGATA CACCTCCCTG 
CTGGATGGCC ACATAATTCA CAGCGTCTGG CTGCAAATCG ACCCCGAGCC GCAAAATAAT 
CCCAGCGAGC TGAACGATCT TAACGGGAAA TCTCTCAGCA TTGCGAGGGC CAAGAACTTC 
GAGTGCGTCG TGAGGAATCT AAAGTCTTTC TTTGAGGAGG AGCTTGGTCA AACCATACTG 
GTCCTACCCG ATGCCTTCAC CCTGGGTCAT CATCCGGAGA GTAAGAACGG GCTCGAACAG 
ATGAAGACCT TGCTGACCCT GTTGCTCGGC GCAGCTGTGC AATGTCCCAA CAAGGAGCTA 
TTTATTGCTC GCATCAAGGA GCTGGACTTA GAAACGCAAC ACGCTATTGT TGGACTGATC 
AAACAAGTGA CCGACAGCCA TAGTTTGGTG CTCACCGAGG ACTCCTTGGA GCGACTTACG 
CCGCAGAGCA TGTACACGCA CATTTTGCGC CTGACCAAGG AGCGGGACGT CATGTATCTC 
AAGTGGATCG ATTTGGCGTG CGTAGAGACC GAAATGACAG CCAGCGATAA TTTGGTGGAA 
TGTGGCCAAG GTGTCAGCGT TACACGTTCC CCTTCGAACG GAACTGCCAC CTCGACACCT 
TCATCTTCTT CCAATTCGGA AAGCAATCAC CTTGCCGTAG AATGTGCCGA TCTGCGTTCG 
AAGAACCGCA AGCTGCGTCA GGAACTCGAG GAAAAGTCCG AGAATCTTTT GGAGCTACGT 
GAGGAGTTGG ATGACAAGAA GGCTCGCTTT GACAAGCTGC GCCAGGAAAG CCAAGAATGG 
TTTACGGAGG CCAAGCGGGC ATCTGCATAT CGTGACGAGG TGGACATCCT CCGGGAACGG 
GCTGAAAGAG CTGATCGCCT GGAGGTGGAA GTTCAGAAGT ACCGCGAGAA ACTCGGAGAC 
TCCGACTTTT ACAAATCCCG CGTTGAGGAA CTGCGGGAGG ATAACCGTGT GCTGCTTGAA 
TCCAAGGAAA TGCTGGAGGA GCAGTTGCAG CGTTACCGAA AAAGGTCTGA GCATGCCATC 
TCTCTGGAAT CCGAGATTAT TAAATACAAA CAGAAGATAA ACGACATGGC TCTCGAACGG 
GACGTGGATC GCTCCAAGCT AGAAGAGTTG CTGGAGGAGA ACTCGCAACT GCAACTGGTA 
GCCAGGAATC TCAACTCCAC GATGGATTTG GATAAATCCT TTTCCGAAAA CGAAGACGAT 
TGCAACTCAG GCGACAATAG TCTTTCGGAA CAGCTAACGA ACAATGCTCA GACCCGTGCA 
CTCAAGCTCG AGCTGGAGAA CCGTCGTTTG ACCGCCGCTC TGGAACAATT AAAAGAGAGT 
AGCTTCCACG AATCCACCAG CAAGATGCTA GAACTGGAAA AGGAGAAGAA AAAGCTGTCT 
CTAAAAATCG AACAGATGCA AGAAAACATC AATCGTCTGA CTCAGCAGAA TGTCGAACTG 
GAAGGGGTCT TTAAAAATGC CCTGGAGGAG AACAAAAAGT TACAGGATGC CGTGGATAAT 
CGCCAAAAAA GCTACGATCG ACAGAGCCTG GAACGCGAAG CTGACCGTCA AAAGCTTTCT 
GATGCCGAGC AGCATGTCGA AACCTTAAAC AAAGAAAAGC AACGTATCCA AACCCTGAAC 
GAAAGCATTC AGCGAAGAGC CGACGATCTT GAACGGTTGG CGGAGAGCAA GACAAAGGAA 
CTAGAGCAAT ACCTGGAAAA ATCAAGGCAA TACGAGCTTA CTAAACAGAA GCTCTATGAG 
ATCGAAGCAA GGGTCTCCAC GTACGAACGC GAAAACGCCA GCCTGCTCAA GGAAGTGTCC 
AAACTCAAGG AGGGCAGCGA GCAAAAGTCT GTGCAGCTGG ATGACAGTAT TAATCGGCTC 
GATGTCCAGA GCAAAGAACT ACAAAAGCTG GGCAAAGCAC TTGAGGATTC GGAGCAAGTG 
CATCAAAAGC TTGTGGAACT GGAGAAGCAA AACCAAGAGC TAGCTTCACA GCGCATCATT 
GATCAAGAGA TGATAAGTAC TCTTCGAAAC GACTTGGTGA CTGGTACTCT CGTAACGAAG 
AAAGTGCGAA ACAACTTGGA GAAACTAGGA TTGGCGGACG AAGAGCCCGG TGAGCTCAAT 
GTGGAGCATG TGGTGGAGAA GTTGGTGCGC AACCCGGAGA CTTTTAAAAC GGTTCGCGAG 
ATAATGCTAA ACGTGACCCG AGAGCAGCTG GAGGAGGAGG AACGAGAAGG TGGCGTCAAG 
TCCGACATGT GTGTGCTATG CCACCGTCAG GAGATCTTTA CGGTGGAAAA GAACATTGAA 
CTCGCGGCTA CTCCAGCTCC TGCACCAGCG CAACCATCTT CGCAGGAACT TCGCTTCGAA 
CACAAGGTAC GTCTAAGTCC AGCACGAGAA TCAGCAGAGC TGACCCGTAT TAAGGATTCC 
AACACTCAGC TTCAGACTGA GAATGCTCGG CTCAGCGTGG ACGTGGCTGC GCTGGGCTCG 
CAAATAACTT CGCTGAACAC CCAGCATGTA GCCCTACAGC TGGCAAACTC CCAGTTGGCT 
GCCGAAAAAG ATTCGCTGCT CAAAGAGATC GATTCACTGC AGCAGGAGCA CAAGCATGCT 
TTGCAGGATC AGGTTACGTT GCAATGCCTG CATGATCAAC TCTCCGCGGA GTACGAGTCT 
CTGAACAAGG ACAAGGAGCA GCTAAAGGCT GCAGTGCGGG ACTTGCGTCA GGAGCTGCGA 
GATACCCGTG AACAGCAGTC GGCATTGGAG CAACGCATTG AGGAGTTGAC TATTCAGAAC 
AGCAACATGA AAACATGCAG CGAGGATCTG TCAATCCTGC GTACGGAGCA CTCCAAACTG 
ACAGACGACT TCCGCAATCT GTTCGCCACC AGCGATCGCT TCAAGAACGA ATACAAAAAC 
ATCCAGGAGC AGTACAAGAT GGTACGCATG GAGCACTCCA GCCTTAAGCT ACAAAACACA 
GAACTCTCCG GCGAGCTAAA TGCTAAGAGC GATCAGGTCC GTTGCCTACA GATGGAGTAT 
AGCAAGGTTC AGCAGCGTTG TGAGATGTTG ATACAAAACA ATGCTGAACT GGACTCGGAG 
CGCAAGGCTC TGATGGACAA CGTGTCCCAA TTGCTCTCTC AATATCAGGA ACTATTGGCC 
ATATCCCTGG AGGACAAAAA GCACTTCCAT GAGGAGGAGA AGAACTACAC CGAACGGGTG 
CACAGCCTCA AGCGGCAGAA GGAAAAACTG GAGGAGAAAA TTATGGAGCA TTACAAGAAG 
TCAGAAACCA CAGTGCACAA GAAAAAACCA TTTGCCAGCA TGCTGGTTAG AAGAGTGAAG 
AAGGCTAGCT CGGATCTAAT GAACAAGGTG CCCAGTCGAA ACCGTCGATC TTGGGTAGAT 
GACGCCCGTA CCAATTCCCA GTTCGTGATC GGTTCCGAGT CAGGCGGCAA TGAGTCGGAT 
AATAGCAATG AGGAGCCACT GTCCATTGCC TCCGACACGC ACCTGCTCCA GAGGAATGTC 
CCGCTCCGCC AGAGTCTGCA GCGCAGTCTT GAAGCGCCAG ACGTGACGGG CAGCAGTCTG 
ACTCTCGGAA CGGCCGGTTC CAGACGCACC GTTTATCTTA TCGATGAGCA CCAAAAGCTT 
CCCGATGGCT CTACTCCCGG CGCCACACAG TCACAGTCTG TTGGCGGCAG TGGCTCCGTC 
AACGCCGCAC CTCCTACACC CGCAGAACCT CAGACGCCTC AGAAGAGTAC CGAAAACAAT 
GCCCCGGTGG GCCCAGCTAC GTTTCTCATG TACAACCGAA TAAACACCAC GATCGGAGGA 
GCCTCGAACA GCGGGGACCA GAGCCCTCTG CTGCAGGCCA GCGGCAGCGT CTCGGGAACG 
ACCATGCAGG ATGACAAGTC GGCAAGGAAG CGGACCGAAG ACAAGAGCAA TAGCATCTGG 
TATGAGTACG GCTGCGTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Girdin ( NM_139523.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_647780.1 Drosophila melanogaster girdin, transcript variant A (Girdin), mRNA.

Price & Availability↑

CloneID	OFa20528
Clone ID Related Accession (Same CDS sequence)	NM_139523.3
Accession Version	NM_139523.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	girdin, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_139523.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTGGCA CTGCGACCGC GACGCCAATG GAAATTGATG AGTTTGTTAA TTCGTCCATA 
ATTTCTTGGC TGGAATCATG TCTGCCACGT GCCGAGCTTC TCGCCGGATA CACCTCCCTG 
CTGGATGGCC ACATAATTCA CAGCGTCTGG CTGCAAATCG ACCCCGAGCC GCAAAATAAT 
CCCAGCGAGC TGAACGATCT TAACGGGAAA TCTCTCAGCA TTGCGAGGGC CAAGAACTTC 
GAGTGCGTCG TGAGGAATCT AAAGTCTTTC TTTGAGGAGG AGCTTGGTCA AACCATACTG 
GTCCTACCCG ATGCCTTCAC CCTGGGTCAT CATCCGGAGA GTAAGAACGG GCTCGAACAG 
ATGAAGACCT TGCTGACCCT GTTGCTCGGC GCAGCTGTGC AATGTCCCAA CAAGGAGCTA 
TTTATTGCTC GCATCAAGGA GCTGGACTTA GAAACGCAAC ACGCTATTGT TGGACTGATC 
AAACAAGTGA CCGACAGCCA TAGTTTGGTG CTCACCGAGG ACTCCTTGGA GCGACTTACG 
CCGCAGAGCA TGTACACGCA CATTTTGCGC CTGACCAAGG AGCGGGACGT CATGTATCTC 
AAGTGGATCG ATTTGGCGTG CGTAGAGACC GAAATGACAG CCAGCGATAA TTTGGTGGAA 
TGTGGCCAAG GTGTCAGCGT TACACGTTCC CCTTCGAACG GAACTGCCAC CTCGACACCT 
TCATCTTCTT CCAATTCGGA AAGCAATCAC CTTGCCGTAG AATGTGCCGA TCTGCGTTCG 
AAGAACCGCA AGCTGCGTCA GGAACTCGAG GAAAAGTCCG AGAATCTTTT GGAGCTACGT 
GAGGAGTTGG ATGACAAGAA GGCTCGCTTT GACAAGCTGC GCCAGGAAAG CCAAGAATGG 
TTTACGGAGG CCAAGCGGGC ATCTGCATAT CGTGACGAGG TGGACATCCT CCGGGAACGG 
GCTGAAAGAG CTGATCGCCT GGAGGTGGAA GTTCAGAAGT ACCGCGAGAA ACTCGGAGAC 
TCCGACTTTT ACAAATCCCG CGTTGAGGAA CTGCGGGAGG ATAACCGTGT GCTGCTTGAA 
TCCAAGGAAA TGCTGGAGGA GCAGTTGCAG CGTTACCGAA AAAGGTCTGA GCATGCCATC 
TCTCTGGAAT CCGAGATTAT TAAATACAAA CAGAAGATAA ACGACATGGC TCTCGAACGG 
GACGTGGATC GCTCCAAGCT AGAAGAGTTG CTGGAGGAGA ACTCGCAACT GCAACTGGTA 
GCCAGGAATC TCAACTCCAC GATGGATTTG GATAAATCCT TTTCCGAAAA CGAAGACGAT 
TGCAACTCAG GCGACAATAG TCTTTCGGAA CAGCTAACGA ACAATGCTCA GACCCGTGCA 
CTCAAGCTCG AGCTGGAGAA CCGTCGTTTG ACCGCCGCTC TGGAACAATT AAAAGAGAGT 
AGCTTCCACG AATCCACCAG CAAGATGCTA GAACTGGAAA AGGAGAAGAA AAAGCTGTCT 
CTAAAAATCG AACAGATGCA AGAAAACATC AATCGTCTGA CTCAGCAGAA TGTCGAACTG 
GAAGGGGTCT TTAAAAATGC CCTGGAGGAG AACAAAAAGT TACAGGATGC CGTGGATAAT 
CGCCAAAAAA GCTACGATCG ACAGAGCCTG GAACGCGAAG CTGACCGTCA AAAGCTTTCT 
GATGCCGAGC AGCATGTCGA AACCTTAAAC AAAGAAAAGC AACGTATCCA AACCCTGAAC 
GAAAGCATTC AGCGAAGAGC CGACGATCTT GAACGGTTGG CGGAGAGCAA GACAAAGGAA 
CTAGAGCAAT ACCTGGAAAA ATCAAGGCAA TACGAGCTTA CTAAACAGAA GCTCTATGAG 
ATCGAAGCAA GGGTCTCCAC GTACGAACGC GAAAACGCCA GCCTGCTCAA GGAAGTGTCC 
AAACTCAAGG AGGGCAGCGA GCAAAAGTCT GTGCAGCTGG ATGACAGTAT TAATCGGCTC 
GATGTCCAGA GCAAAGAACT ACAAAAGCTG GGCAAAGCAC TTGAGGATTC GGAGCAAGTG 
CATCAAAAGC TTGTGGAACT GGAGAAGCAA AACCAAGAGC TAGCTTCACA GCGCATCATT 
GATCAAGAGA TGATAAGTAC TCTTCGAAAC GACTTGGTGA CTGGTACTCT CGTAACGAAG 
AAAGTGCGAA ACAACTTGGA GAAACTAGGA TTGGCGGACG AAGAGCCCGG TGAGCTCAAT 
GTGGAGCATG TGGTGGAGAA GTTGGTGCGC AACCCGGAGA CTTTTAAAAC GGTTCGCGAG 
ATAATGCTAA ACGTGACCCG AGAGCAGCTG GAGGAGGAGG AACGAGAAGG TGGCGTCAAG 
TCCGACATGT GTGTGCTATG CCACCGTCAG GAGATCTTTA CGGTGGAAAA GAACATTGAA 
CTCGCGGCTA CTCCAGCTCC TGCACCAGCG CAACCATCTT CGCAGGAACT TCGCTTCGAA 
CACAAGGTAC GTCTAAGTCC AGCACGAGAA TCAGCAGAGC TGACCCGTAT TAAGGATTCC 
AACACTCAGC TTCAGACTGA GAATGCTCGG CTCAGCGTGG ACGTGGCTGC GCTGGGCTCG 
CAAATAACTT CGCTGAACAC CCAGCATGTA GCCCTACAGC TGGCAAACTC CCAGTTGGCT 
GCCGAAAAAG ATTCGCTGCT CAAAGAGATC GATTCACTGC AGCAGGAGCA CAAGCATGCT 
TTGCAGGATC AGGTTACGTT GCAATGCCTG CATGATCAAC TCTCCGCGGA GTACGAGTCT 
CTGAACAAGG ACAAGGAGCA GCTAAAGGCT GCAGTGCGGG ACTTGCGTCA GGAGCTGCGA 
GATACCCGTG AACAGCAGTC GGCATTGGAG CAACGCATTG AGGAGTTGAC TATTCAGAAC 
AGCAACATGA AAACATGCAG CGAGGATCTG TCAATCCTGC GTACGGAGCA CTCCAAACTG 
ACAGACGACT TCCGCAATCT GTTCGCCACC AGCGATCGCT TCAAGAACGA ATACAAAAAC 
ATCCAGGAGC AGTACAAGAT GGTACGCATG GAGCACTCCA GCCTTAAGCT ACAAAACACA 
GAACTCTCCG GCGAGCTAAA TGCTAAGAGC GATCAGGTCC GTTGCCTACA GATGGAGTAT 
AGCAAGGTTC AGCAGCGTTG TGAGATGTTG ATACAAAACA ATGCTGAACT GGACTCGGAG 
CGCAAGGCTC TGATGGACAA CGTGTCCCAA TTGCTCTCTC AATATCAGGA ACTATTGGCC 
ATATCCCTGG AGGACAAAAA GCACTTCCAT GAGGAGGAGA AGAACTACAC CGAACGGGTG 
CACAGCCTCA AGCGGCAGAA GGAAAAACTG GAGGAGAAAA TTATGGAGCA TTACAAGAAG 
TCAGAAACCA CAGTGCACAA GAAAAAACCA TTTGCCAGCA TGCTGGTTAG AAGAGTGAAG 
AAGGCTAGCT CGGATCTAAT GAACAAGGTG CCCAGTCGAA ACCGTCGATC TTGGGTAGAT 
GACGCCCGTA CCAATTCCCA GTTCGTGATC GGTTCCGAGT CAGGCGGCAA TGAGTCGGAT 
AATAGCAATG AGGAGCCACT GTCCATTGCC TCCGACACGC ACCTGCTCCA GAGGAATGTC 
CCGCTCCGCC AGAGTCTGCA GCGGGATTTG CTGGATAACT CGATCCAACG CGGCGGTGCC 
GTGCGCAGTA GCCTGCAGGC TCAGAAACGT ACGGATTTGA ATAACTCACG TAGAAATAGC 
GTGCACGGCA GTCTTGAAGC GCCAGACGTG ACGGGCAGCA GTCTGACTCT CGGAACGGCC 
GGTTCCAGAC GCACCGTTTA TCTTATCGAT GAGCACCAAA AGCTTCCCGA TGGCTCTACT 
CCCGGCGCCA CACAGTCACA GTCTGTTGGC GGCAGTGGCT CCGTCAACGC CGCACCTCCT 
ACACCCGCAG AACCTCAGAC GCCTCAGAAG AGTACCGAAA ACAATGCCCC GGTGGGCCCA 
GCTACGTTTC TCATGTACAA CCGAATAAAC ACCACGATCG GAGGAGCCTC GAACAGCGGG 
GACCAGAGCC CTCTGCTGCA GGCCAGCGGC AGCGTCTCGG GAACGACCAT GCAGGATGAC 
AAGTCGGCAA GGAAGCGGAC CGAAGACAAG AGCAATAGCA TCTGGTATGA GTACGGCTGC 
GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_647780.1
CDS	424..4569
Translation	MAGTATATPMEIDEFVNSSIISWLESCLPRAELLAGYTSLLDGHIIHSVWLQIDPEPQNNPSELNDLNGKSLSIARAKNFECVVRNLKSFFEEELGQTILVLPDAFTLGHHPESKNGLEQMKTLLTLLLGAAVQCPNKELFIARIKELDLETQHAIVGLIKQVTDSHSLVLTEDSLERLTPQSMYTHILRLTKERDVMYLKWIDLACVETEMTASDNLVECGQGVSVTRSPSNGTATSTPSSSSNSESNHLAVECADLRSKNRKLRQELEEKSENLLELREELDDKKARFDKLRQESQEWFTEAKRASAYRDEVDILRERAERADRLEVEVQKYREKLGDSDFYKSRVEELREDNRVLLESKEMLEEQLQRYRKRSEHAISLESEIIKYKQKINDMALERDVDRSKLEELLEENSQLQLVARNLNSTMDLDKSFSENEDDCNSGDNSLSEQLTNNAQTRALKLELENRRLTAALEQLKESSFHESTSKMLELEKEKKKLSLKIEQMQENINRLTQQNVELEGVFKNALEENKKLQDAVDNRQKSYDRQSLEREADRQKLSDAEQHVETLNKEKQRIQTLNESIQRRADDLERLAESKTKELEQYLEKSRQYELTKQKLYEIEARVSTYERENASLLKEVSKLKEGSEQKSVQLDDSINRLDVQSKELQKLGKALEDSEQVHQKLVELEKQNQELASQRIIDQEMISTLRNDLVTGTLVTKKVRNNLEKLGLADEEPGELNVEHVVEKLVRNPETFKTVREIMLNVTREQLEEEEREGGVKSDMCVLCHRQEIFTVEKNIELAATPAPAPAQPSSQELRFEHKVRLSPARESAELTRIKDSNTQLQTENARLSVDVAALGSQITSLNTQHVALQLANSQLAAEKDSLLKEIDSLQQEHKHALQDQVTLQCLHDQLSAEYESLNKDKEQLKAAVRDLRQELRDTREQQSALEQRIEELTIQNSNMKTCSEDLSILRTEHSKLTDDFRNLFATSDRFKNEYKNIQEQYKMVRMEHSSLKLQNTELSGELNAKSDQVRCLQMEYSKVQQRCEMLIQNNAELDSERKALMDNVSQLLSQYQELLAISLEDKKHFHEEEKNYTERVHSLKRQKEKLEEKIMEHYKKSETTVHKKKPFASMLVRRVKKASSDLMNKVPSRNRRSWVDDARTNSQFVIGSESGGNESDNSNEEPLSIASDTHLLQRNVPLRQSLQRDLLDNSIQRGGAVRSSLQAQKRTDLNNSRRNSVHGSLEAPDVTGSSLTLGTAGSRRTVYLIDEHQKLPDGSTPGATQSQSVGGSGSVNAAPPTPAEPQTPQKSTENNAPVGPATFLMYNRINTTIGGASNSGDQSPLLQASGSVSGTTMQDDKSARKRTEDKSNSIWYEYGCV

Target ORF information:

RefSeq Version	NM_139523.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster girdin, transcript variant A (Girdin), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139523.3

ORF Insert Sequence:

ATGGCTGGCA CTGCGACCGC GACGCCAATG GAAATTGATG AGTTTGTTAA TTCGTCCATA 
ATTTCTTGGC TGGAATCATG TCTGCCACGT GCCGAGCTTC TCGCCGGATA CACCTCCCTG 
CTGGATGGCC ACATAATTCA CAGCGTCTGG CTGCAAATCG ACCCCGAGCC GCAAAATAAT 
CCCAGCGAGC TGAACGATCT TAACGGGAAA TCTCTCAGCA TTGCGAGGGC CAAGAACTTC 
GAGTGCGTCG TGAGGAATCT AAAGTCTTTC TTTGAGGAGG AGCTTGGTCA AACCATACTG 
GTCCTACCCG ATGCCTTCAC CCTGGGTCAT CATCCGGAGA GTAAGAACGG GCTCGAACAG 
ATGAAGACCT TGCTGACCCT GTTGCTCGGC GCAGCTGTGC AATGTCCCAA CAAGGAGCTA 
TTTATTGCTC GCATCAAGGA GCTGGACTTA GAAACGCAAC ACGCTATTGT TGGACTGATC 
AAACAAGTGA CCGACAGCCA TAGTTTGGTG CTCACCGAGG ACTCCTTGGA GCGACTTACG 
CCGCAGAGCA TGTACACGCA CATTTTGCGC CTGACCAAGG AGCGGGACGT CATGTATCTC 
AAGTGGATCG ATTTGGCGTG CGTAGAGACC GAAATGACAG CCAGCGATAA TTTGGTGGAA 
TGTGGCCAAG GTGTCAGCGT TACACGTTCC CCTTCGAACG GAACTGCCAC CTCGACACCT 
TCATCTTCTT CCAATTCGGA AAGCAATCAC CTTGCCGTAG AATGTGCCGA TCTGCGTTCG 
AAGAACCGCA AGCTGCGTCA GGAACTCGAG GAAAAGTCCG AGAATCTTTT GGAGCTACGT 
GAGGAGTTGG ATGACAAGAA GGCTCGCTTT GACAAGCTGC GCCAGGAAAG CCAAGAATGG 
TTTACGGAGG CCAAGCGGGC ATCTGCATAT CGTGACGAGG TGGACATCCT CCGGGAACGG 
GCTGAAAGAG CTGATCGCCT GGAGGTGGAA GTTCAGAAGT ACCGCGAGAA ACTCGGAGAC 
TCCGACTTTT ACAAATCCCG CGTTGAGGAA CTGCGGGAGG ATAACCGTGT GCTGCTTGAA 
TCCAAGGAAA TGCTGGAGGA GCAGTTGCAG CGTTACCGAA AAAGGTCTGA GCATGCCATC 
TCTCTGGAAT CCGAGATTAT TAAATACAAA CAGAAGATAA ACGACATGGC TCTCGAACGG 
GACGTGGATC GCTCCAAGCT AGAAGAGTTG CTGGAGGAGA ACTCGCAACT GCAACTGGTA 
GCCAGGAATC TCAACTCCAC GATGGATTTG GATAAATCCT TTTCCGAAAA CGAAGACGAT 
TGCAACTCAG GCGACAATAG TCTTTCGGAA CAGCTAACGA ACAATGCTCA GACCCGTGCA 
CTCAAGCTCG AGCTGGAGAA CCGTCGTTTG ACCGCCGCTC TGGAACAATT AAAAGAGAGT 
AGCTTCCACG AATCCACCAG CAAGATGCTA GAACTGGAAA AGGAGAAGAA AAAGCTGTCT 
CTAAAAATCG AACAGATGCA AGAAAACATC AATCGTCTGA CTCAGCAGAA TGTCGAACTG 
GAAGGGGTCT TTAAAAATGC CCTGGAGGAG AACAAAAAGT TACAGGATGC CGTGGATAAT 
CGCCAAAAAA GCTACGATCG ACAGAGCCTG GAACGCGAAG CTGACCGTCA AAAGCTTTCT 
GATGCCGAGC AGCATGTCGA AACCTTAAAC AAAGAAAAGC AACGTATCCA AACCCTGAAC 
GAAAGCATTC AGCGAAGAGC CGACGATCTT GAACGGTTGG CGGAGAGCAA GACAAAGGAA 
CTAGAGCAAT ACCTGGAAAA ATCAAGGCAA TACGAGCTTA CTAAACAGAA GCTCTATGAG 
ATCGAAGCAA GGGTCTCCAC GTACGAACGC GAAAACGCCA GCCTGCTCAA GGAAGTGTCC 
AAACTCAAGG AGGGCAGCGA GCAAAAGTCT GTGCAGCTGG ATGACAGTAT TAATCGGCTC 
GATGTCCAGA GCAAAGAACT ACAAAAGCTG GGCAAAGCAC TTGAGGATTC GGAGCAAGTG 
CATCAAAAGC TTGTGGAACT GGAGAAGCAA AACCAAGAGC TAGCTTCACA GCGCATCATT 
GATCAAGAGA TGATAAGTAC TCTTCGAAAC GACTTGGTGA CTGGTACTCT CGTAACGAAG 
AAAGTGCGAA ACAACTTGGA GAAACTAGGA TTGGCGGACG AAGAGCCCGG TGAGCTCAAT 
GTGGAGCATG TGGTGGAGAA GTTGGTGCGC AACCCGGAGA CTTTTAAAAC GGTTCGCGAG 
ATAATGCTAA ACGTGACCCG AGAGCAGCTG GAGGAGGAGG AACGAGAAGG TGGCGTCAAG 
TCCGACATGT GTGTGCTATG CCACCGTCAG GAGATCTTTA CGGTGGAAAA GAACATTGAA 
CTCGCGGCTA CTCCAGCTCC TGCACCAGCG CAACCATCTT CGCAGGAACT TCGCTTCGAA 
CACAAGGTAC GTCTAAGTCC AGCACGAGAA TCAGCAGAGC TGACCCGTAT TAAGGATTCC 
AACACTCAGC TTCAGACTGA GAATGCTCGG CTCAGCGTGG ACGTGGCTGC GCTGGGCTCG 
CAAATAACTT CGCTGAACAC CCAGCATGTA GCCCTACAGC TGGCAAACTC CCAGTTGGCT 
GCCGAAAAAG ATTCGCTGCT CAAAGAGATC GATTCACTGC AGCAGGAGCA CAAGCATGCT 
TTGCAGGATC AGGTTACGTT GCAATGCCTG CATGATCAAC TCTCCGCGGA GTACGAGTCT 
CTGAACAAGG ACAAGGAGCA GCTAAAGGCT GCAGTGCGGG ACTTGCGTCA GGAGCTGCGA 
GATACCCGTG AACAGCAGTC GGCATTGGAG CAACGCATTG AGGAGTTGAC TATTCAGAAC 
AGCAACATGA AAACATGCAG CGAGGATCTG TCAATCCTGC GTACGGAGCA CTCCAAACTG 
ACAGACGACT TCCGCAATCT GTTCGCCACC AGCGATCGCT TCAAGAACGA ATACAAAAAC 
ATCCAGGAGC AGTACAAGAT GGTACGCATG GAGCACTCCA GCCTTAAGCT ACAAAACACA 
GAACTCTCCG GCGAGCTAAA TGCTAAGAGC GATCAGGTCC GTTGCCTACA GATGGAGTAT 
AGCAAGGTTC AGCAGCGTTG TGAGATGTTG ATACAAAACA ATGCTGAACT GGACTCGGAG 
CGCAAGGCTC TGATGGACAA CGTGTCCCAA TTGCTCTCTC AATATCAGGA ACTATTGGCC 
ATATCCCTGG AGGACAAAAA GCACTTCCAT GAGGAGGAGA AGAACTACAC CGAACGGGTG 
CACAGCCTCA AGCGGCAGAA GGAAAAACTG GAGGAGAAAA TTATGGAGCA TTACAAGAAG 
TCAGAAACCA CAGTGCACAA GAAAAAACCA TTTGCCAGCA TGCTGGTTAG AAGAGTGAAG 
AAGGCTAGCT CGGATCTAAT GAACAAGGTG CCCAGTCGAA ACCGTCGATC TTGGGTAGAT 
GACGCCCGTA CCAATTCCCA GTTCGTGATC GGTTCCGAGT CAGGCGGCAA TGAGTCGGAT 
AATAGCAATG AGGAGCCACT GTCCATTGCC TCCGACACGC ACCTGCTCCA GAGGAATGTC 
CCGCTCCGCC AGAGTCTGCA GCGGGATTTG CTGGATAACT CGATCCAACG CGGCGGTGCC 
GTGCGCAGTA GCCTGCAGGC TCAGAAACGT ACGGATTTGA ATAACTCACG TAGAAATAGC 
GTGCACGGCA GTCTTGAAGC GCCAGACGTG ACGGGCAGCA GTCTGACTCT CGGAACGGCC 
GGTTCCAGAC GCACCGTTTA TCTTATCGAT GAGCACCAAA AGCTTCCCGA TGGCTCTACT 
CCCGGCGCCA CACAGTCACA GTCTGTTGGC GGCAGTGGCT CCGTCAACGC CGCACCTCCT 
ACACCCGCAG AACCTCAGAC GCCTCAGAAG AGTACCGAAA ACAATGCCCC GGTGGGCCCA 
GCTACGTTTC TCATGTACAA CCGAATAAAC ACCACGATCG GAGGAGCCTC GAACAGCGGG 
GACCAGAGCC CTCTGCTGCA GGCCAGCGGC AGCGTCTCGG GAACGACCAT GCAGGATGAC 
AAGTCGGCAA GGAAGCGGAC CGAAGACAAG AGCAATAGCA TCTGGTATGA GTACGGCTGC 
GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Girdin cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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