Svil gene cDNA ORF clone, Drosophila melanogaster(fruit fly)

mRNA	Protein	Name
NM_001202101.1	NP_001189030.1	supervillin, isoform M
NM_001202102.2	NP_001189031.1	supervillin, isoform G
NM_001202103.2	NP_001189032.1	supervillin, isoform H
NM_001169859.2	NP_001163330.1	supervillin, isoform F
NM_167948.1	NP_728744.1	supervillin, isoform A
NM_001259639.1	NP_001246568.1	supervillin, isoform S
NM_206246.3	NP_995968.2	supervillin, isoform X
NM_001299989.1	NP_001286918.1	supervillin, isoform AD
NM_167949.1	NP_728745.1	supervillin, isoform C
NM_001202104.2	NP_001189033.2	supervillin, isoform P
NM_001259637.1	NP_001246566.1	supervillin, isoform Q
NM_001299986.1	NP_001286915.1	supervillin, isoform W
NM_001299987.1	NP_001286916.1	supervillin, isoform AB
NM_001299990.1	NP_001286919.1	supervillin, isoform AE
NM_001202097.2	NP_001189026.1	supervillin, isoform AH
NM_001202100.2	NP_001189029.1	supervillin, isoform N
NM_001259638.1	NP_001246567.1	supervillin, isoform R
NM_001031933.3	NP_001027104.1	supervillin, isoform Y
NM_001274400.1	NP_001261329.1	supervillin, isoform AA
NM_206242.3	NP_995964.2	supervillin, isoform L
NM_139464.4	NP_647721.3	supervillin, isoform K
NM_001169858.2	NP_001163329.1	supervillin, isoform E
NM_001259636.1	NP_001246565.1	supervillin, isoform O
NM_206244.3	NP_995966.1	supervillin, isoform Z
NM_001299988.1	NP_001286917.1	supervillin, isoform AC
NM_139462.4	NP_647719.2	supervillin, isoform AF
NM_001202098.2	NP_001189027.1	supervillin, isoform AG
NM_001299985.1	NP_001286914.1	supervillin, isoform V
NM_001202105.2	NP_001189034.1	supervillin, isoform J
NM_001274398.2	NP_001261327.1	supervillin, isoform T
NM_001274399.1	NP_001261328.1	supervillin, isoform U

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa17297	NM_167948.1 Latest version!	Drosophila melanogaster supervillin, transcript variant A (Svil), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa17298	NM_167949.1 Latest version!	Drosophila melanogaster supervillin, transcript variant C (Svil), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa20002	NM_001259636.1 Latest version!	Drosophila melanogaster supervillin, transcript variant O (Svil), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa20003	NM_001202104.2 Latest version!	Drosophila melanogaster supervillin, transcript variant P (Svil), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa20004	NM_001202105.2 Latest version!	Drosophila melanogaster supervillin, transcript variant J (Svil), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa19636	NM_001274400.1 Latest version!	Drosophila melanogaster supervillin, transcript variant AA (Svil), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa20086	NM_001031933.3 Latest version!	Drosophila melanogaster supervillin, transcript variant Y (Svil), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa20086	NM_206244.3 Latest version!	Drosophila melanogaster supervillin, transcript variant Z (Svil), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa17294	NM_139462.4 Latest version!	Drosophila melanogaster supervillin, transcript variant AF (Svil), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa19994	NM_001202097.2 Latest version!	Drosophila melanogaster supervillin, transcript variant AH (Svil), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa19994	NM_001202098.2 Latest version!	Drosophila melanogaster supervillin, transcript variant AG (Svil), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa19996	NM_001169859.2 Latest version!	Drosophila melanogaster supervillin, transcript variant F (Svil), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa21979	NM_206246.3 Latest version!	Drosophila melanogaster supervillin, transcript variant X (Svil), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa17295	NM_001274398.2 Latest version!	Drosophila melanogaster supervillin, transcript variant T (Svil), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OFa17295	NM_001259637.1 Latest version!	Drosophila melanogaster supervillin, transcript variant Q (Svil), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OFa17295	NM_001202101.1 Latest version!	Drosophila melanogaster supervillin, transcript variant M (Svil), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OFa17295	NM_206242.3 Latest version!	Drosophila melanogaster supervillin, transcript variant L (Svil), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OFa17296	NM_139464.4 Latest version!	Drosophila melanogaster supervillin, transcript variant K (Svil), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OFa19995	NM_001202100.2 Latest version!	Drosophila melanogaster supervillin, transcript variant N (Svil), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OFa19997	NM_001259638.1 Latest version!	Drosophila melanogaster supervillin, transcript variant R (Svil), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OFa19997	NM_001169858.2 Latest version!	Drosophila melanogaster supervillin, transcript variant E (Svil), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OFa19998	NM_001259639.1 Latest version!	Drosophila melanogaster supervillin, transcript variant S (Svil), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OFa19999	NM_001274399.1 Latest version!	Drosophila melanogaster supervillin, transcript variant U (Svil), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OFa20000	NM_001202102.2 Latest version!	Drosophila melanogaster supervillin, transcript variant G (Svil), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OFa20001	NM_001202103.2 Latest version!	Drosophila melanogaster supervillin, transcript variant H (Svil), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OFa21973	NM_001299988.1 Latest version!	Drosophila melanogaster supervillin, transcript variant AC (Svil), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa21974	NM_001299990.1 Latest version!	Drosophila melanogaster supervillin, transcript variant AE (Svil), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa21975	NM_001299987.1 Latest version!	Drosophila melanogaster supervillin, transcript variant AB (Svil), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa21976	NM_001299989.1 Latest version!	Drosophila melanogaster supervillin, transcript variant AD (Svil), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa21977	NM_001299986.1 Latest version!	Drosophila melanogaster supervillin, transcript variant W (Svil), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa21978	NM_001299985.1 Latest version!	Drosophila melanogaster supervillin, transcript variant V (Svil), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00

Svil ( NM_167948.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728744.1 Drosophila melanogaster supervillin, transcript variant A (Svil), mRNA.

Price & Availability↑

CloneID	OFa17297
Clone ID Related Accession (Same CDS sequence)	NM_167948.1
Accession Version	NM_167948.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1428bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	supervillin, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCTACCA CAATCAAGCG GCACAGTACA ACCATTGTAA TCAAATCATC CATTTCAGGA 
ACCACCAACT CCCGTCAATC TCCACCAGCG TCGTCGGCGG AGGTGTCTTC AAGTGCAGCC 
GACAATTCCA TGCACAGTGA CGAGCGGTCC ACGCCAAATG CAACGATAGA TGCGGAATTC 
GAACCCTCAT CCCTGTCGCT GGCTGAGCGA CTGGCCTTCT TCAGCAATCT GTGCGAGAGC 
GGCGGAGGAG GTGGGAGTAC CAGTGCCGGT GGAAGGTACC GCAGTCGCAC CAGCAGCTAT 
TCAAGGAGTC CGCCCGGCGA TCGCACCACT CCCAGGAGCA GCACCACCGC CAGCAGTGTC 
AGTATGACGC CCACGCCCAT GGATTACTCT CCGATTCCCC ACGACAAGGA GCCATCTAGT 
CTCACGCTGG AGAGCATCAT CGAGCCCGAG CAGGAGGAGG TCAAGGAGCA GGTGGACCAG 
GATGAGGTCA AGTTGAGGAA GAAGGAGCCA GTCCAGAGGG AATCTTCCCA GCACAATGGC 
TTCCACGTGC TGACCAGGTC CGCCACCGAA CCGCCGTCGT CCACCTCGCC CCTGCGCATC 
AGCATACGGA CTGTGGGAAA GCTAGTCCTG CCCGATACCT TCCGCGGCGA TCGCAACAAT 
AACAGCAGTA GTAGAAGTGG GAGCAACAGC TGGAGCAACA CTTGCATGGT CAAGCTGCAG 
AGCTTGGAGC CCGTTAGCCT GTCCGTTCAT TCGCGCACCA TTGGTAAGGT TAAGTCGCCA 
TTCATTGAAA AGCAGCAGGA GAAACCACAC ACCGAGAAGC TAGCGAACGG AACCAGCGAC 
AGTGGCTCCG ATTCGGGCAA GGAGAACTGC GCCTCCAACG AGCAGGAACA TCTGCAGCAC 
CAGCAGTCGC AGGACGAGGA TCGAGCTCCG CCCAGGGTGT CCGAGATGCG GAGGAAAATC 
AGCCGACTGG TGCAACAACA GCAGCCGCAA CCACAGCCCG TCAATCGGCG GCACACCACC 
GAGATCACCT CCGTGACCGT TGGAACGCGT TCGCCCAGTG TGGACGATCG CTTTGCCAAG 
TACTTTGGTG TTAAGGAGAG CTGCAACAGC ACGACCACCG TGCATAAAAC GCAATCGCTG 
CCACCCAGCC AAGTTGAGGC CACCAATGCG CACGTGAAAC TACCGCAGAT CACTACCGTG 
GTTTCCAAAC GGCGCGAGCT GCTCAAGAGG ACGCGACCCC TGTCCATGGA TCACTACTCC 
AGCGGCTGCA CCAGTCCCAC AGCCATGCCA AGTCCCAAGA GAGCACATTC GCCCGTCGGC 
CGGCGGAAGG CCGCCATTTC CAGCATCGAG GACATTGAGG TCACGCCGGA TGAACTCCGG 
GCCGCTGGCA GGAACTTCAA GCTGTTGTAC GGCGAGTTAT TGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728744.1
CDS	111..1538
Translation	MPTTIKRHSTTIVIKSSISGTTNSRQSPPASSAEVSSSAADNSMHSDERSTPNATIDAEFEPSSLSLAERLAFFSNLCESGGGGGSTSAGGRYRSRTSSYSRSPPGDRTTPRSSTTASSVSMTPTPMDYSPIPHDKEPSSLTLESIIEPEQEEVKEQVDQDEVKLRKKEPVQRESSQHNGFHVLTRSATEPPSSTSPLRISIRTVGKLVLPDTFRGDRNNNSSSRSGSNSWSNTCMVKLQSLEPVSLSVHSRTIGKVKSPFIEKQQEKPHTEKLANGTSDSGSDSGKENCASNEQEHLQHQQSQDEDRAPPRVSEMRRKISRLVQQQQPQPQPVNRRHTTEITSVTVGTRSPSVDDRFAKYFGVKESCNSTTTVHKTQSLPPSQVEATNAHVKLPQITTVVSKRRELLKRTRPLSMDHYSSGCTSPTAMPSPKRAHSPVGRRKAAISSIEDIEVTPDELRAAGRNFKLLYGELLG

Target ORF information:

RefSeq Version	NM_167948.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster supervillin, transcript variant A (Svil), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167948.1

ORF Insert Sequence:

ATGCCTACCA CAATCAAGCG GCACAGTACA ACCATTGTAA TCAAATCATC CATTTCAGGA 
ACCACCAACT CCCGTCAATC TCCACCAGCG TCGTCGGCGG AGGTGTCTTC AAGTGCAGCC 
GACAATTCCA TGCACAGTGA CGAGCGGTCC ACGCCAAATG CAACGATAGA TGCGGAATTC 
GAACCCTCAT CCCTGTCGCT GGCTGAGCGA CTGGCCTTCT TCAGCAATCT GTGCGAGAGC 
GGCGGAGGAG GTGGGAGTAC CAGTGCCGGT GGAAGGTACC GCAGTCGCAC CAGCAGCTAT 
TCAAGGAGTC CGCCCGGCGA TCGCACCACT CCCAGGAGCA GCACCACCGC CAGCAGTGTC 
AGTATGACGC CCACGCCCAT GGATTACTCT CCGATTCCCC ACGACAAGGA GCCATCTAGT 
CTCACGCTGG AGAGCATCAT CGAGCCCGAG CAGGAGGAGG TCAAGGAGCA GGTGGACCAG 
GATGAGGTCA AGTTGAGGAA GAAGGAGCCA GTCCAGAGGG AATCTTCCCA GCACAATGGC 
TTCCACGTGC TGACCAGGTC CGCCACCGAA CCGCCGTCGT CCACCTCGCC CCTGCGCATC 
AGCATACGGA CTGTGGGAAA GCTAGTCCTG CCCGATACCT TCCGCGGCGA TCGCAACAAT 
AACAGCAGTA GTAGAAGTGG GAGCAACAGC TGGAGCAACA CTTGCATGGT CAAGCTGCAG 
AGCTTGGAGC CCGTTAGCCT GTCCGTTCAT TCGCGCACCA TTGGTAAGGT TAAGTCGCCA 
TTCATTGAAA AGCAGCAGGA GAAACCACAC ACCGAGAAGC TAGCGAACGG AACCAGCGAC 
AGTGGCTCCG ATTCGGGCAA GGAGAACTGC GCCTCCAACG AGCAGGAACA TCTGCAGCAC 
CAGCAGTCGC AGGACGAGGA TCGAGCTCCG CCCAGGGTGT CCGAGATGCG GAGGAAAATC 
AGCCGACTGG TGCAACAACA GCAGCCGCAA CCACAGCCCG TCAATCGGCG GCACACCACC 
GAGATCACCT CCGTGACCGT TGGAACGCGT TCGCCCAGTG TGGACGATCG CTTTGCCAAG 
TACTTTGGTG TTAAGGAGAG CTGCAACAGC ACGACCACCG TGCATAAAAC GCAATCGCTG 
CCACCCAGCC AAGTTGAGGC CACCAATGCG CACGTGAAAC TACCGCAGAT CACTACCGTG 
GTTTCCAAAC GGCGCGAGCT GCTCAAGAGG ACGCGACCCC TGTCCATGGA TCACTACTCC 
AGCGGCTGCA CCAGTCCCAC AGCCATGCCA AGTCCCAAGA GAGCACATTC GCCCGTCGGC 
CGGCGGAAGG CCGCCATTTC CAGCATCGAG GACATTGAGG TCACGCCGGA TGAACTCCGG 
GCCGCTGGCA GGAACTTCAA GCTGTTGTAC GGCGAGTTAT TGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Svil ( NM_167949.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728745.1 Drosophila melanogaster supervillin, transcript variant C (Svil), mRNA.

Price & Availability↑

CloneID	OFa17298
Clone ID Related Accession (Same CDS sequence)	NM_167949.1
Accession Version	NM_167949.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	supervillin, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAACCCT CATCCCTGTC GCTGGCTGAG CGACTGGCCT TCTTCAGCAA TCTGTGCGAG 
AGCGGCGGAG GAGGTGGGAG TACCAGTGCC GGTGGAAGGT ACCGCAGTCG CACCAGCAGC 
TATTCAAGGA GTCCGCCCGG CGATCGCACC ACTCCCAGGA GCAGCACCAC CGCCAGCAGT 
GTCAGTATGA CGCCCACGCC CATGGATTAC TCTCCGATTC CCCACGACAA GGAGCCATCT 
AGTCTCACGC TGGAGAGCAT CATCGAGCCC GAGCAGGAGG AGGTCAAGGA GCAGGTGGAC 
CAGGATGAGG TCAAGTTGAG GAAGAAGGAG CCAGTCCAGA GGGAATCTTC CCAGCACAAT 
GGCTTCCACG TGCTGACCAG GTCCGCCACC GAACCGCCGT CGTCCACCTC GCCCCTGCGC 
ATCAGCATAC GGACTGTGGG AAAGCTAGTC CTGCCCGATA CCTTCCGCGG CGATCGCAAC 
AATAACAGCA GTAGTAGAAG TGGGAGCAAC AGCTGGAGCA ACACTTGCAT GGTCAAGCTG 
CAGAGCTTGG AGCCCGTTAG CCTGTCCGTT CATTCGCGCA CCATTGGTAA GGTTAAGTCG 
CCATTCATTG AAAAGCAGCA GGAGAAACCA CACACCGAGA AGCTAGCGAA CGGAACCAGC 
GACAGTGGCT CCGATTCGGG CAAGGAGAAC TGCGCCTCCA ACGAGCAGGA ACATCTGCAG 
CACCAGCAGT CGCAGGACGA GGATCGAGCT CCGCCCAGGG TGTCCGAGAT GCGGAGGAAA 
ATCAGCCGAC TGGTGCAACA ACAGCAGCCG CAACCACAGC CCGTCAATCG GCGGCACACC 
ACCGAGATCA CCTCCGTGAC CGTTGGAACG CGTTCGCCCA GTGTGGACGA TCGCTTTGCC 
AAGTACTTTG GTGTTAAGGA GAGCTGCAAC AGCACGACCA CCGTGCATAA AACGCAATCG 
CTGCCACCCA GCCAAGTTGA GGCCACCAAT GCGCACGTGA AACTACCGCA GATCACTACC 
GTGGTTTCCA AACGGCGCGA GCTGCTCAAG AGGACGCGAC CCCTGTCCAT GGATCACTAC 
TCCAGCGGCT GCACCAGTCC CACAGCCATG CCAAGTCCCA AGAGAGCACA TTCGCCCGTC 
GGCCGGCGGA AGGCCGCCAT TTCCAGCATC GAGGACATTG AGGTCACGCC GGATGAACTC 
CGGGCCGCTG GCAGGAACTT CAAGCTGTTG TACGGCGAGT TATTGGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728745.1
CDS	121..1371
Translation	MQPSSLSLAERLAFFSNLCESGGGGGSTSAGGRYRSRTSSYSRSPPGDRTTPRSSTTASSVSMTPTPMDYSPIPHDKEPSSLTLESIIEPEQEEVKEQVDQDEVKLRKKEPVQRESSQHNGFHVLTRSATEPPSSTSPLRISIRTVGKLVLPDTFRGDRNNNSSSRSGSNSWSNTCMVKLQSLEPVSLSVHSRTIGKVKSPFIEKQQEKPHTEKLANGTSDSGSDSGKENCASNEQEHLQHQQSQDEDRAPPRVSEMRRKISRLVQQQQPQPQPVNRRHTTEITSVTVGTRSPSVDDRFAKYFGVKESCNSTTTVHKTQSLPPSQVEATNAHVKLPQITTVVSKRRELLKRTRPLSMDHYSSGCTSPTAMPSPKRAHSPVGRRKAAISSIEDIEVTPDELRAAGRNFKLLYGELLG

Target ORF information:

RefSeq Version	NM_167949.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster supervillin, transcript variant C (Svil), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167949.1

ORF Insert Sequence:

ATGCAACCCT CATCCCTGTC GCTGGCTGAG CGACTGGCCT TCTTCAGCAA TCTGTGCGAG 
AGCGGCGGAG GAGGTGGGAG TACCAGTGCC GGTGGAAGGT ACCGCAGTCG CACCAGCAGC 
TATTCAAGGA GTCCGCCCGG CGATCGCACC ACTCCCAGGA GCAGCACCAC CGCCAGCAGT 
GTCAGTATGA CGCCCACGCC CATGGATTAC TCTCCGATTC CCCACGACAA GGAGCCATCT 
AGTCTCACGC TGGAGAGCAT CATCGAGCCC GAGCAGGAGG AGGTCAAGGA GCAGGTGGAC 
CAGGATGAGG TCAAGTTGAG GAAGAAGGAG CCAGTCCAGA GGGAATCTTC CCAGCACAAT 
GGCTTCCACG TGCTGACCAG GTCCGCCACC GAACCGCCGT CGTCCACCTC GCCCCTGCGC 
ATCAGCATAC GGACTGTGGG AAAGCTAGTC CTGCCCGATA CCTTCCGCGG CGATCGCAAC 
AATAACAGCA GTAGTAGAAG TGGGAGCAAC AGCTGGAGCA ACACTTGCAT GGTCAAGCTG 
CAGAGCTTGG AGCCCGTTAG CCTGTCCGTT CATTCGCGCA CCATTGGTAA GGTTAAGTCG 
CCATTCATTG AAAAGCAGCA GGAGAAACCA CACACCGAGA AGCTAGCGAA CGGAACCAGC 
GACAGTGGCT CCGATTCGGG CAAGGAGAAC TGCGCCTCCA ACGAGCAGGA ACATCTGCAG 
CACCAGCAGT CGCAGGACGA GGATCGAGCT CCGCCCAGGG TGTCCGAGAT GCGGAGGAAA 
ATCAGCCGAC TGGTGCAACA ACAGCAGCCG CAACCACAGC CCGTCAATCG GCGGCACACC 
ACCGAGATCA CCTCCGTGAC CGTTGGAACG CGTTCGCCCA GTGTGGACGA TCGCTTTGCC 
AAGTACTTTG GTGTTAAGGA GAGCTGCAAC AGCACGACCA CCGTGCATAA AACGCAATCG 
CTGCCACCCA GCCAAGTTGA GGCCACCAAT GCGCACGTGA AACTACCGCA GATCACTACC 
GTGGTTTCCA AACGGCGCGA GCTGCTCAAG AGGACGCGAC CCCTGTCCAT GGATCACTAC 
TCCAGCGGCT GCACCAGTCC CACAGCCATG CCAAGTCCCA AGAGAGCACA TTCGCCCGTC 
GGCCGGCGGA AGGCCGCCAT TTCCAGCATC GAGGACATTG AGGTCACGCC GGATGAACTC 
CGGGCCGCTG GCAGGAACTT CAAGCTGTTG TACGGCGAGT TATTGGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Svil ( NM_001259636.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246565.1 Drosophila melanogaster supervillin, transcript variant O (Svil), mRNA.

Price & Availability↑

CloneID	OFa20002
Clone ID Related Accession (Same CDS sequence)	NM_001259636.1
Accession Version	NM_001259636.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1224bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	supervillin, isoform O
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTATTGC TGCAGCGACG TCAGAGCGAA AAAATGTTTG AGGCGTATAT CAGAAAACGC 
TTCAAAGCCA ACTCCGCGCC CTTCGACATC TCGGCTACAT CGGCCTTCAA GCCCACCAAG 
CCGCTGGACA TCCGCTTGAC CCACGCACCG CATGCACCGC TGATTTCGCC GATCACCGGA 
CAGCCACTCA GCCACGTGCC CGCGCCACTG CTGCTATCCT CGTCGACGAA CTCGGGAGCG 
GGATCCTCGT TCCGTCTGGC CCGCAGCTCC ACCCAACCGC TGCAGTCGCC GCGAGTTGTC 
CACCTGGCCG GTCAGGCTAC TGGTGGCTCC GCTTTGGCAC GCCAGAACTC GGTGAACGAC 
AGCATCAATC TCAGCGAGCA GCTAACCCTA TCCATCGGCA CGGACAGCGA GCACATCTTT 
GAGATGTCCG CTTTGGAGGA GGACTCGGCT ATTCAGTCGC TGAGCGACGA GACAGCCGCT 
TCCGCATCTG CCAACTCAAC ATCCACGACC ACTGCAACCA CCATGACACC GCCCAGTGGC 
CTGACGCGGA GCAACAGCGT TCGGGCCAGG GCCAATATGT TCCAGCAATT GCAGGAGCAG 
TCGCGCAACC AACGGGCAAC GGGAACCACC AACTCCCGTC AATCTCCACC AGCGTCGTCG 
GCGGAGGTGT CTTCAAGTGC AGCCGACAAT TCCATGCACA GTGACGAGCG GTCCACGCCA 
AATGCAACGA TAGATGCGGA ATTCGGAAAA CCCAGCACCG AACCACTTAA GAGCATTCTT 
AAGACGGGAA TGCCAGTGAT GGGTATGGGA CGCGGTTTAA TGCCTGTGGA TCTCAATGCC 
GAGCTGAAGA ACCGGCTCAA ACGCTCCACA CATGCCACTG TTTCCAATCT CCGCAAATCT 
GCCACCTCGG CAGATGCCAC TAGAGCAGCT AGTGATGAGG TGGACAATCC GCAGAGGAAT 
TTAGCCCAGA TCCTGAGGAA TGTTTCCAAG GAGAACTCCA CGCCGAAGCA CGATGATGCC 
GTGAGCCTAG TGCGGAATCT CTCTACTTTG GGACAACCTC GATCGCTGGC CCGTGACGAT 
GCAGCCGGGA ATTGTGCCTC GGCATCCGAA GGCGAGAGTT CGGGGGGCCG CGAGATCGAG 
GCCATAATCA AGAACAGCGC TGTGGCCAGA CGTCGCCGGC AGCAACAGCA GCAGTCGCAG 
CAGCAGAATC AGCCAGATGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246565.1
CDS	252..1475
Translation	MLLLQRRQSEKMFEAYIRKRFKANSAPFDISATSAFKPTKPLDIRLTHAPHAPLISPITGQPLSHVPAPLLLSSSTNSGAGSSFRLARSSTQPLQSPRVVHLAGQATGGSALARQNSVNDSINLSEQLTLSIGTDSEHIFEMSALEEDSAIQSLSDETAASASANSTSTTTATTMTPPSGLTRSNSVRARANMFQQLQEQSRNQRATGTTNSRQSPPASSAEVSSSAADNSMHSDERSTPNATIDAEFGKPSTEPLKSILKTGMPVMGMGRGLMPVDLNAELKNRLKRSTHATVSNLRKSATSADATRAASDEVDNPQRNLAQILRNVSKENSTPKHDDAVSLVRNLSTLGQPRSLARDDAAGNCASASEGESSGGREIEAIIKNSAVARRRRQQQQQSQQQNQPDG

Target ORF information:

RefSeq Version	NM_001259636.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster supervillin, transcript variant O (Svil), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259636.1

ORF Insert Sequence:

ATGTTATTGC TGCAGCGACG TCAGAGCGAA AAAATGTTTG AGGCGTATAT CAGAAAACGC 
TTCAAAGCCA ACTCCGCGCC CTTCGACATC TCGGCTACAT CGGCCTTCAA GCCCACCAAG 
CCGCTGGACA TCCGCTTGAC CCACGCACCG CATGCACCGC TGATTTCGCC GATCACCGGA 
CAGCCACTCA GCCACGTGCC CGCGCCACTG CTGCTATCCT CGTCGACGAA CTCGGGAGCG 
GGATCCTCGT TCCGTCTGGC CCGCAGCTCC ACCCAACCGC TGCAGTCGCC GCGAGTTGTC 
CACCTGGCCG GTCAGGCTAC TGGTGGCTCC GCTTTGGCAC GCCAGAACTC GGTGAACGAC 
AGCATCAATC TCAGCGAGCA GCTAACCCTA TCCATCGGCA CGGACAGCGA GCACATCTTT 
GAGATGTCCG CTTTGGAGGA GGACTCGGCT ATTCAGTCGC TGAGCGACGA GACAGCCGCT 
TCCGCATCTG CCAACTCAAC ATCCACGACC ACTGCAACCA CCATGACACC GCCCAGTGGC 
CTGACGCGGA GCAACAGCGT TCGGGCCAGG GCCAATATGT TCCAGCAATT GCAGGAGCAG 
TCGCGCAACC AACGGGCAAC GGGAACCACC AACTCCCGTC AATCTCCACC AGCGTCGTCG 
GCGGAGGTGT CTTCAAGTGC AGCCGACAAT TCCATGCACA GTGACGAGCG GTCCACGCCA 
AATGCAACGA TAGATGCGGA ATTCGGAAAA CCCAGCACCG AACCACTTAA GAGCATTCTT 
AAGACGGGAA TGCCAGTGAT GGGTATGGGA CGCGGTTTAA TGCCTGTGGA TCTCAATGCC 
GAGCTGAAGA ACCGGCTCAA ACGCTCCACA CATGCCACTG TTTCCAATCT CCGCAAATCT 
GCCACCTCGG CAGATGCCAC TAGAGCAGCT AGTGATGAGG TGGACAATCC GCAGAGGAAT 
TTAGCCCAGA TCCTGAGGAA TGTTTCCAAG GAGAACTCCA CGCCGAAGCA CGATGATGCC 
GTGAGCCTAG TGCGGAATCT CTCTACTTTG GGACAACCTC GATCGCTGGC CCGTGACGAT 
GCAGCCGGGA ATTGTGCCTC GGCATCCGAA GGCGAGAGTT CGGGGGGCCG CGAGATCGAG 
GCCATAATCA AGAACAGCGC TGTGGCCAGA CGTCGCCGGC AGCAACAGCA GCAGTCGCAG 
CAGCAGAATC AGCCAGATGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Svil ( NM_001202104.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001189033.2 Drosophila melanogaster supervillin, transcript variant P (Svil), mRNA.

Price & Availability↑

CloneID	OFa20003
Clone ID Related Accession (Same CDS sequence)	NM_001202104.2
Accession Version	NM_001202104.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	supervillin, isoform P
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001202104.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTATTGC TGCAGCGACG TCAGAGCGAA AAAATGTTTG AGGCGTATAT CAGAAAACGC 
TTCAAAGCCA ACTCCGCGCC CTTCGACATC TCGGCTACAT CGGCCTTCAA GCCCACCAAG 
CCGCTGGACA TCCGCTTGAC CCACGCACCG CATGCACCGC TGATTTCGCC GATCACCGGA 
CAGCCACTCA GCCACGTGCC CGCGCCACTG CTGCTATCCT CGTCGACGAA CTCGGGAGCG 
GGATCCTCGT TCCGTCTGGC CCGCAGCTCC ACCCAACCGC TGCAGTCGCC GCGAGTTGTC 
CACCTGGCCG GTCAGGCTAC TGGTGGCTCC GCTTTGGCAC GCCAGAACTC GGTGAACGAC 
AGCATCAATC TCAGCGAGCA GCTAACCCTA TCCATCGGCA CGGACAGCGA GCACATCTTT 
GAGATGTCCG CTTTGGAGGA GGACTCGGCT ATTCAGTCGC TGAGCGACGA GACAGCCGCT 
TCCGCATCTG CCAACTCAAC ATCCACGACC ACTGCAACCA CCATGACACC GCCCAGTGGC 
CTGACGCGGA GCAACAGCGT TCGGGCCAGG GCCAATATGT TCCAGCAATT GCAGGAGCAG 
TCGCGCAACC AACGGGCAAC GGGAACCACC AACTCCCGTC AATCTCCACC AGCGTCGTCG 
GCGGAGGTGT CTTCAAGTGC AGCCGACAAT TCCATGCACA GTGACGAGCG GTCCACGCCA 
AATGCAACGA TAGATGCGGA ATTCGAACCC TCATCCCTGT CGCTGGCTGA GCGACTGGCC 
TTCTTCAGCA ATCTGTGCGA GAGCGGCGGA GGAGGTGGGA GTACCAGTGC CGGTGGAAGG 
TACCGCAGTC GCACCAGCAG CTATTCAAGG AGTCCGCCCG GCGATCGCAC CACTCCCAGG 
AGCAGCACCA CCGCCAGCAG TGTCAGTATG ACGCCCACGC CCATGGATTA CTCTCCGATT 
CCCCACGACA AGGAGCCATC TAGTCTCACG CTGGAGAGCA TCATCGAGCC CGAGCAGGAG 
GAGGTCAAGG AGCAGGTGGA CCAGGATGAG GTCAAGTTGA GGAAGAAGGA GCCAGTCCAG 
AGGGAATCTT CCCAGCACAA TGGCTTCCAC GTGCTGACCA GGTCCGCCAC CGAACCGCCG 
TCGTCCACCT CGCCCCTGCG CATCAGCATA CGGACTGTGG GAAAGCTAGT CCTGCCCGAT 
ACCTTCCGCG GCGATCGCAA CAATAACAGC AGTAGTAGAA GTGGGAGCAA CAGCTGGAGC 
AACACTTGCA TGGTCAAGCT GCAGAGCTTG GAGCCCGTTA GCCTGTCCGT TCATTCGCGC 
ACCATTGGTA AGGTTAAGTC GCCATTCATT GAAAAGCAGC AGGAGAAACC ACACACCGAG 
AAGCTAGCGA ACGGAACCAG CGACAGTGGC TCCGATTCGG GCAAGGAGAA CTGCGCCTCC 
AACGAGCAGG AACATCTGCA GCACCAGCAG TCGCAGGACG AGGATCGAGC TCCGCCCAGG 
GTGTCCGAGA TGCGGAGGAA AATCAGCCGA CTGGTGCAAC AACAGCAGCC GCAACCACAG 
CCCGTCAATC GGCGGCACAC CACCGAGATC ACCTCCGTGA CCGTTGGAAC GCGTTCGCCC 
AGTGTGGACG ATCGCTTTGC CAAGTACTTT GGTGTTAAGG AGAGCTGCAA CAGCACGACC 
ACCGTGCATA AAACGCAATC GCTGCCACCC AGCCAAGTTG AGGCCACCAA TGCGCACGTG 
AAACTACCGC AGATCACTAC CGTGGTTTCC AAACGGCGCG AGCTGCTCAA GAGGACGCGA 
CCCCTGTCCA TGGATCACTA CTCCAGCGGC TGCACCAGTC CCACAGCCAT GCCAAGTCCC 
AAGAGAGCAC ATTCGCCCGT CGGCCGGCGG AAGGCCGCCA TTTCCAGCAT CGAGGACATT 
GAGGTCACGC CGGATGAACT CCGGGCCGCT GGCAGGAACT TCAAGCTGTT GTACGGCGAG 
TTATTGGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189033.2
CDS	252..2243
Translation	MLLLQRRQSEKMFEAYIRKRFKANSAPFDISATSAFKPTKPLDIRLTHAPHAPLISPITGQPLSHVPAPLLLSSSTNSGAGSSFRLARSSTQPLQSPRVVHLAGQATGGSALARQNSVNDSINLSEQLTLSIGTDSEHIFEMSALEEDSAIQSLSDETAASASANSTSTTTATTMTPPSGLTRSNSVRARANMFQQLQEQSRNQRATGTTNSRQSPPASSAEVSSSAADNSMHSDERSTPNATIDAEFEPSSLSLAERLAFFSNLCESGGGGGSTSAGGRYRSRTSSYSRSPPGDRTTPRSSTTASSVSMTPTPMDYSPIPHDKEPSSLTLESIIEPEQEEVKEQVDQDEVKLRKKEPVQRESSQHNGFHVLTRSATEPPSSTSPLRISIRTVGKLVLPDTFRGDRNNNSSSRSGSNSWSNTCMVKLQSLEPVSLSVHSRTIGKVKSPFIEKQQEKPHTEKLANGTSDSGSDSGKENCASNEQEHLQHQQSQDEDRAPPRVSEMRRKISRLVQQQQPQPQPVNRRHTTEITSVTVGTRSPSVDDRFAKYFGVKESCNSTTTVHKTQSLPPSQVEATNAHVKLPQITTVVSKRRELLKRTRPLSMDHYSSGCTSPTAMPSPKRAHSPVGRRKAAISSIEDIEVTPDELRAAGRNFKLLYGELLG

Target ORF information:

RefSeq Version	NM_001202104.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster supervillin, transcript variant P (Svil), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202104.2

ORF Insert Sequence:

ATGTTATTGC TGCAGCGACG TCAGAGCGAA AAAATGTTTG AGGCGTATAT CAGAAAACGC 
TTCAAAGCCA ACTCCGCGCC CTTCGACATC TCGGCTACAT CGGCCTTCAA GCCCACCAAG 
CCGCTGGACA TCCGCTTGAC CCACGCACCG CATGCACCGC TGATTTCGCC GATCACCGGA 
CAGCCACTCA GCCACGTGCC CGCGCCACTG CTGCTATCCT CGTCGACGAA CTCGGGAGCG 
GGATCCTCGT TCCGTCTGGC CCGCAGCTCC ACCCAACCGC TGCAGTCGCC GCGAGTTGTC 
CACCTGGCCG GTCAGGCTAC TGGTGGCTCC GCTTTGGCAC GCCAGAACTC GGTGAACGAC 
AGCATCAATC TCAGCGAGCA GCTAACCCTA TCCATCGGCA CGGACAGCGA GCACATCTTT 
GAGATGTCCG CTTTGGAGGA GGACTCGGCT ATTCAGTCGC TGAGCGACGA GACAGCCGCT 
TCCGCATCTG CCAACTCAAC ATCCACGACC ACTGCAACCA CCATGACACC GCCCAGTGGC 
CTGACGCGGA GCAACAGCGT TCGGGCCAGG GCCAATATGT TCCAGCAATT GCAGGAGCAG 
TCGCGCAACC AACGGGCAAC GGGAACCACC AACTCCCGTC AATCTCCACC AGCGTCGTCG 
GCGGAGGTGT CTTCAAGTGC AGCCGACAAT TCCATGCACA GTGACGAGCG GTCCACGCCA 
AATGCAACGA TAGATGCGGA ATTCGAACCC TCATCCCTGT CGCTGGCTGA GCGACTGGCC 
TTCTTCAGCA ATCTGTGCGA GAGCGGCGGA GGAGGTGGGA GTACCAGTGC CGGTGGAAGG 
TACCGCAGTC GCACCAGCAG CTATTCAAGG AGTCCGCCCG GCGATCGCAC CACTCCCAGG 
AGCAGCACCA CCGCCAGCAG TGTCAGTATG ACGCCCACGC CCATGGATTA CTCTCCGATT 
CCCCACGACA AGGAGCCATC TAGTCTCACG CTGGAGAGCA TCATCGAGCC CGAGCAGGAG 
GAGGTCAAGG AGCAGGTGGA CCAGGATGAG GTCAAGTTGA GGAAGAAGGA GCCAGTCCAG 
AGGGAATCTT CCCAGCACAA TGGCTTCCAC GTGCTGACCA GGTCCGCCAC CGAACCGCCG 
TCGTCCACCT CGCCCCTGCG CATCAGCATA CGGACTGTGG GAAAGCTAGT CCTGCCCGAT 
ACCTTCCGCG GCGATCGCAA CAATAACAGC AGTAGTAGAA GTGGGAGCAA CAGCTGGAGC 
AACACTTGCA TGGTCAAGCT GCAGAGCTTG GAGCCCGTTA GCCTGTCCGT TCATTCGCGC 
ACCATTGGTA AGGTTAAGTC GCCATTCATT GAAAAGCAGC AGGAGAAACC ACACACCGAG 
AAGCTAGCGA ACGGAACCAG CGACAGTGGC TCCGATTCGG GCAAGGAGAA CTGCGCCTCC 
AACGAGCAGG AACATCTGCA GCACCAGCAG TCGCAGGACG AGGATCGAGC TCCGCCCAGG 
GTGTCCGAGA TGCGGAGGAA AATCAGCCGA CTGGTGCAAC AACAGCAGCC GCAACCACAG 
CCCGTCAATC GGCGGCACAC CACCGAGATC ACCTCCGTGA CCGTTGGAAC GCGTTCGCCC 
AGTGTGGACG ATCGCTTTGC CAAGTACTTT GGTGTTAAGG AGAGCTGCAA CAGCACGACC 
ACCGTGCATA AAACGCAATC GCTGCCACCC AGCCAAGTTG AGGCCACCAA TGCGCACGTG 
AAACTACCGC AGATCACTAC CGTGGTTTCC AAACGGCGCG AGCTGCTCAA GAGGACGCGA 
CCCCTGTCCA TGGATCACTA CTCCAGCGGC TGCACCAGTC CCACAGCCAT GCCAAGTCCC 
AAGAGAGCAC ATTCGCCCGT CGGCCGGCGG AAGGCCGCCA TTTCCAGCAT CGAGGACATT 
GAGGTCACGC CGGATGAACT CCGGGCCGCT GGCAGGAACT TCAAGCTGTT GTACGGCGAG 
TTATTGGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Svil ( NM_001202105.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001189034.1 Drosophila melanogaster supervillin, transcript variant J (Svil), mRNA.

Price & Availability↑

CloneID	OFa20004
Clone ID Related Accession (Same CDS sequence)	NM_001202105.2
Accession Version	NM_001202105.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	supervillin, isoform J
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001202105.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCGCTT TGGAGGAGGA CTCGGCTATT CAGTCGCTGA GCGACGAGAC AGCCGCTTCC 
GCATCTGCCA ACTCAACATC CACGACCACT GCAACCACCA TGACACCGCC CAGTGGCCTG 
ACGCGGAGCA ACAGCGTTCG GGCCAGGGCC AATATGTTCC AGCAATTGCA GGAGCAGTCG 
CGCAACCAAC GGGCAACGGG AACCACCAAC TCCCGTCAAT CTCCACCAGC GTCGTCGGCG 
GAGGTGTCTT CAAGTGCAGC CGACAATTCC ATGCACAGTG ACGAGCGGTC CACGCCAAAT 
GCAACGATAG ATGCGGAATT CGGAAAACCC AGCACCGAAC CACTTAAGAG CATTCTTAAG 
ACGGGAATGC CAGTGATGGG TATGGGACGC GGTTTAATGC CTGTGGATCT CAATGCCGAG 
CTGAAGAACC GGCTCAAACG CTCCACACAT GCCACTGTTT CCAATCTCCG CAAATCTGCC 
ACCTCGGCAG ATGCCACTAG AGCAGCTAGT GATGAGGTGG ACAATCCGCA GAGGAATTTA 
GCCCAGATCC TGAGGAATGT TTCCAAGGAG AACTCCACGC CGAAGCACGA TGATGCCGTG 
AGCCTAGTGC GGAATCTCTC TACTTTGGGA CAACCTCGAT CGCTGGCCCG TGACGATGCA 
GCCGGGAATT GTGCCTCGGC ATCCGAAGGC GAGAGTTCGG GGGGCCGCGA GATCGAGGCC 
ATAATCAAGA ACAGCGCTGT GGCCAGACGT CGCCGGCAGC AACAGCAGCA GTCGCAGCAG 
CAGAATCAGC CAGATGGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189034.1
CDS	738..1538
Translation	MSALEEDSAIQSLSDETAASASANSTSTTTATTMTPPSGLTRSNSVRARANMFQQLQEQSRNQRATGTTNSRQSPPASSAEVSSSAADNSMHSDERSTPNATIDAEFGKPSTEPLKSILKTGMPVMGMGRGLMPVDLNAELKNRLKRSTHATVSNLRKSATSADATRAASDEVDNPQRNLAQILRNVSKENSTPKHDDAVSLVRNLSTLGQPRSLARDDAAGNCASASEGESSGGREIEAIIKNSAVARRRRQQQQQSQQQNQPDG

Target ORF information:

RefSeq Version	NM_001202105.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster supervillin, transcript variant J (Svil), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202105.2

ORF Insert Sequence:

ATGTCCGCTT TGGAGGAGGA CTCGGCTATT CAGTCGCTGA GCGACGAGAC AGCCGCTTCC 
GCATCTGCCA ACTCAACATC CACGACCACT GCAACCACCA TGACACCGCC CAGTGGCCTG 
ACGCGGAGCA ACAGCGTTCG GGCCAGGGCC AATATGTTCC AGCAATTGCA GGAGCAGTCG 
CGCAACCAAC GGGCAACGGG AACCACCAAC TCCCGTCAAT CTCCACCAGC GTCGTCGGCG 
GAGGTGTCTT CAAGTGCAGC CGACAATTCC ATGCACAGTG ACGAGCGGTC CACGCCAAAT 
GCAACGATAG ATGCGGAATT CGGAAAACCC AGCACCGAAC CACTTAAGAG CATTCTTAAG 
ACGGGAATGC CAGTGATGGG TATGGGACGC GGTTTAATGC CTGTGGATCT CAATGCCGAG 
CTGAAGAACC GGCTCAAACG CTCCACACAT GCCACTGTTT CCAATCTCCG CAAATCTGCC 
ACCTCGGCAG ATGCCACTAG AGCAGCTAGT GATGAGGTGG ACAATCCGCA GAGGAATTTA 
GCCCAGATCC TGAGGAATGT TTCCAAGGAG AACTCCACGC CGAAGCACGA TGATGCCGTG 
AGCCTAGTGC GGAATCTCTC TACTTTGGGA CAACCTCGAT CGCTGGCCCG TGACGATGCA 
GCCGGGAATT GTGCCTCGGC ATCCGAAGGC GAGAGTTCGG GGGGCCGCGA GATCGAGGCC 
ATAATCAAGA ACAGCGCTGT GGCCAGACGT CGCCGGCAGC AACAGCAGCA GTCGCAGCAG 
CAGAATCAGC CAGATGGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Svil ( NM_001274400.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261329.1 Drosophila melanogaster supervillin, transcript variant AA (Svil), mRNA.

Price & Availability↑

CloneID	OFa19636
Clone ID Related Accession (Same CDS sequence)	NM_001274400.1
Accession Version	NM_001274400.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	supervillin, isoform AA
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAAAAG TAAAACTGGA ATCGCTATCT TTGGCCCACT CGCTGTCGGA CAAACCACTG 
CCCCCATCAC CTTTGATACC CACCAGCTTG GAGGGCGGCA AGGTCTCCGA TCGTTTGAGC 
AAACTCAAGT GCAACTCGGA GAACTGGAAG CAGCGCATAG AGCAAACCGA TGCCAAGAAA 
TTCACAGTCG CCGGTCGACT GCAAAAGAAG GCCCAATCCC CCGTGGAGCT TCAATTCGAG 
CGTTCTGCCA ATGATGCGGC CAAAAAGTGT CCCATGCTGG AGGTGCGCAG CGCCAATCAG 
CCGCAGCTTG GGCTGGCCAA GAGCCCCTCC ATGATGGTCA CCTCTAGCAC CAGCACCAAT 
AGCACCAGCA AGTCCGCACT CCGCAGCCTG AGCGTCAATC CCGAGGAGGA GGGACCCACC 
AGCCTGAGTC GCTCCAACAG CAGCAGTTCG GAGTCCGATG GAGAGCGCAA ATCCCCCCAG 
AATGCCAAGC AGAACAAGGA GCTGGCCAAC AACAAGAGTG CTGCAGCACC CACCAATGTG 
GGAGCCAGGA TTCAGGTGCC CCGGCTGGAC GACAAGGAAA CTTTCGAGAA CTTCTTCTCC 
TCCAAGCCGA AGAAGGAGGA GAACGTTCAG CTGGAGATCA GCGCTTTCGA TGACATCAAG 
CCCACTGAGC GCTTGGTAAG CAAGCGACAC GTACAGGGTC CCAAAGGTCG ACGGGCTGCA 
CGCAATCCTC TCAAGAGTTT AGCTGCTCGT GAGGACATTA GCTCCGAGTA CACCGAAATG 
AGGTCGGGCA TAGCCGAGCG AGAATTACGG CGCCTAAAGT TGGAGTCATA TGGTCATAAT 
GCCAACTTGG CTGCAGAGGC CATAGCCGGA TTAGCCTCCG TCGAGGACTT CAAGTCGGTG 
GCACTGCGCT CCTCGTCGAT TCCACTGCAG CAAATGTGGC TACCCCACAA GCCAGTGATG 
TTACTCCATG TTAAGGGGCG CACACATGTC CAAACCCGAC TGGTGGAACC CGTGCATACC 
TCCCTGAACC GTGGTGATTG CTTCATCCTG GTGGCCGGAT CGCAGCTTTT CCGCTACGTT 
GGATCCTTTG CAAACGTAAT CGAGATCTCC CGGAGCAAGA AGATCTGCGC TGCCATAGTG 
GAAAACAAGG ATCTCGGTTG CACCGCCACC CAGGAGGTGA TCCTCACGGA TGGCAAGTAT 
TTGAAGGAGC GCCAGTGGCG TCAGTTCTGG CAGCTTTTGG GCAAAAAAGA GGATGATCAA 
TCGGATATAG CTGAATGCGG TCATGCCGAT GAAGACGACG TGTTCGAGAG CAGCCTGATC 
GAGACTAACA AGATCTACGA GTACCAGGAT GAGGCGCTCA TTCCGTTGGA GAAATTCTGG 
GGCTGCATCC CCAAGGTGGA CATGTTGGAC ACGCGTAAAG TTTTGGTCTT TGACTTTGGC 
AGCGAGCTGT ATGTGTGGAA TGGCAAGAAT GCCCCTTCCG ATGCCAAGCG AGCTGCCATG 
AGATTGGCCC AGGAACACTT TGGTGCTGAG AATTCGGCGG ACTATGCCGA CTGTTACTTG 
AATCCCCTGA ACTATGTCGC CATAGTGGGT CGTCGTGAGA ACACCAAGTA TGCCAAGAGG 
TGCGCACAGC GTCCAGAATG GTGTGTTCTC GGCAAGATCA CCCAGAACAT GGAGACGGTG 
CTGTTCAAGG AGAAGTTCAG TGATTGGCCA GAGATAGAAC GCGAGGATCT GGAGAAGGAT 
TACCTATCAA ACGGAGTGCA TATGGTGCGT GCTTTAAACG GCACCGCTTT GCATAAGGGA 
GAACCCTACC AGGAACCAAA TCTGGTGCTC GAGCAGGCCA ACTTGGGGCG TGGCAATTTC 
TACTACGACA CGGACACCAT GCGGCACTTC GATGTGATTA CCAAGTCAAC GGACAAATGG 
CAGATCCACG AGTTCAACTT TGATGCGGCG AATAATCGCG ATGACTACGG TCACTTCTAC 
TCGGCGGAGA GCTATATAGT GCGCTGGATC TACCAGATTT CGGTCACAGT TCGCGAACTC 
AGTGGCAAGG TGTCGAACAG AAGCACAGTG GGTCGAGACA GGTGTGTCTA TTTCACCTGG 
CAAGGACAGG ACTCAAGTGC CAACGAGAAG GGTGCAGCTG CTTTGCTGAC CGTAGAGTTG 
GACAAGGAGA AAGGCGCACA GATGCGGGTG TCGCAAGGAG ATGAGTGCAC AGCCTTCGTC 
CGCCTGTTCC GGCAAATGTG GCAACATCGT GGACGCAAGG AGCAATGTCT GGAGAGGCAC 
AGCGAGTGGA GGCTCTACCA ACTGCAGGGC AACGTCAGCG AGGAGACCAT CATCAAGGAA 
GTCGTCTGCC AGTCCAGCCA TTTGCGATCC CGCAGCTCCA TGCTGTTAAT TCACGGCAGT 
CAGGGAAATG TGATTGTCTG GCATGGATCG AAGAGTGCTC CTCATACGCG TAATGTGGCT 
GTAGGAGTTG CTCAGGATCT GATCAAGACG GTGCCTAAAG ATCTCTTCAG CGCCGAAACT 
GCCAACCTAA CTGAGGTGGA GGAGGGTTCC GACGACGATC AGTGCAAGAA GGCCCTGGGA 
TTGAAGGATG AAAGAAATGA ATATGGCAGC CTGCTTAAGT CCACCAAGTC GTTCGATTAC 
CAAATAAGGA TCTTCAACTT CTCCAGCACT CAGGGTGTTT TCAAAGCCCT CGAACTGAGC 
GATCCGCTGC GCTGCCAGGA TCTGCATAGT CCGTATCCCT TCAGCCAGTC GCAGCTGTAC 
AATGCAAGGC AGCCGACTAT CTTTTTGCTG GACGACGGCG ATGAGTTGTG GCTCTGGATG 
GGATGGTGGC CTCTGGAAGA TGTGAAGATA AACACCGATG AGCGAAGCAG TCCCACCAAC 
GAAAATCGCG CTGGAGTAAA TCGCTGGATC TCAGAGAGAC GTGCTGCCCT CGAGACAGCC 
GTTGACTATT GGCGTGCCAA GCACGGCGAG AACGAGAATG AACCCTTCCA TGGCATCAAA 
GGCCAGGTTG TGTGGGCAGG CCTGGAGCCA CTGGCCTTTA AGGCGCTGTT TCCCGACTGG 
ACAGAACGTC CGGATGTAAG AGAAATCAAC GAAGAAGATG GGCGAACTGA TGCTTCCATA 
TCGATCGCCA CAATGCTGGC ACAAATGACG CAGACCGAGT ATCCCCTGGA AGTCCTGAAA 
GCCCGACCGC TGCCCGAAGG TGTGGAGCCC ACCCGCCTGG AGGTCTACCT TAATGCGGAG 
GACTTCCAGG CGGCACTGGG ATGCAGCCGG GCGGAATTCG AGCAGCTGCC CATCTGGAAA 
CAGACCAAGC TGAAGAAGGA GCGAGGGCTG TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261329.1
CDS	211..3546
Translation	MEKVKLESLSLAHSLSDKPLPPSPLIPTSLEGGKVSDRLSKLKCNSENWKQRIEQTDAKKFTVAGRLQKKAQSPVELQFERSANDAAKKCPMLEVRSANQPQLGLAKSPSMMVTSSTSTNSTSKSALRSLSVNPEEEGPTSLSRSNSSSSESDGERKSPQNAKQNKELANNKSAAAPTNVGARIQVPRLDDKETFENFFSSKPKKEENVQLEISAFDDIKPTERLVSKRHVQGPKGRRAARNPLKSLAAREDISSEYTEMRSGIAERELRRLKLESYGHNANLAAEAIAGLASVEDFKSVALRSSSIPLQQMWLPHKPVMLLHVKGRTHVQTRLVEPVHTSLNRGDCFILVAGSQLFRYVGSFANVIEISRSKKICAAIVENKDLGCTATQEVILTDGKYLKERQWRQFWQLLGKKEDDQSDIAECGHADEDDVFESSLIETNKIYEYQDEALIPLEKFWGCIPKVDMLDTRKVLVFDFGSELYVWNGKNAPSDAKRAAMRLAQEHFGAENSADYADCYLNPLNYVAIVGRRENTKYAKRCAQRPEWCVLGKITQNMETVLFKEKFSDWPEIEREDLEKDYLSNGVHMVRALNGTALHKGEPYQEPNLVLEQANLGRGNFYYDTDTMRHFDVITKSTDKWQIHEFNFDAANNRDDYGHFYSAESYIVRWIYQISVTVRELSGKVSNRSTVGRDRCVYFTWQGQDSSANEKGAAALLTVELDKEKGAQMRVSQGDECTAFVRLFRQMWQHRGRKEQCLERHSEWRLYQLQGNVSEETIIKEVVCQSSHLRSRSSMLLIHGSQGNVIVWHGSKSAPHTRNVAVGVAQDLIKTVPKDLFSAETANLTEVEEGSDDDQCKKALGLKDERNEYGSLLKSTKSFDYQIRIFNFSSTQGVFKALELSDPLRCQDLHSPYPFSQSQLYNARQPTIFLLDDGDELWLWMGWWPLEDVKINTDERSSPTNENRAGVNRWISERRAALETAVDYWRAKHGENENEPFHGIKGQVVWAGLEPLAFKALFPDWTERPDVREINEEDGRTDASISIATMLAQMTQTEYPLEVLKARPLPEGVEPTRLEVYLNAEDFQAALGCSRAEFEQLPIWKQTKLKKERGLF

Target ORF information:

RefSeq Version	NM_001274400.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster supervillin, transcript variant AA (Svil), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274400.1

ORF Insert Sequence:

ATGGAAAAAG TAAAACTGGA ATCGCTATCT TTGGCCCACT CGCTGTCGGA CAAACCACTG 
CCCCCATCAC CTTTGATACC CACCAGCTTG GAGGGCGGCA AGGTCTCCGA TCGTTTGAGC 
AAACTCAAGT GCAACTCGGA GAACTGGAAG CAGCGCATAG AGCAAACCGA TGCCAAGAAA 
TTCACAGTCG CCGGTCGACT GCAAAAGAAG GCCCAATCCC CCGTGGAGCT TCAATTCGAG 
CGTTCTGCCA ATGATGCGGC CAAAAAGTGT CCCATGCTGG AGGTGCGCAG CGCCAATCAG 
CCGCAGCTTG GGCTGGCCAA GAGCCCCTCC ATGATGGTCA CCTCTAGCAC CAGCACCAAT 
AGCACCAGCA AGTCCGCACT CCGCAGCCTG AGCGTCAATC CCGAGGAGGA GGGACCCACC 
AGCCTGAGTC GCTCCAACAG CAGCAGTTCG GAGTCCGATG GAGAGCGCAA ATCCCCCCAG 
AATGCCAAGC AGAACAAGGA GCTGGCCAAC AACAAGAGTG CTGCAGCACC CACCAATGTG 
GGAGCCAGGA TTCAGGTGCC CCGGCTGGAC GACAAGGAAA CTTTCGAGAA CTTCTTCTCC 
TCCAAGCCGA AGAAGGAGGA GAACGTTCAG CTGGAGATCA GCGCTTTCGA TGACATCAAG 
CCCACTGAGC GCTTGGTAAG CAAGCGACAC GTACAGGGTC CCAAAGGTCG ACGGGCTGCA 
CGCAATCCTC TCAAGAGTTT AGCTGCTCGT GAGGACATTA GCTCCGAGTA CACCGAAATG 
AGGTCGGGCA TAGCCGAGCG AGAATTACGG CGCCTAAAGT TGGAGTCATA TGGTCATAAT 
GCCAACTTGG CTGCAGAGGC CATAGCCGGA TTAGCCTCCG TCGAGGACTT CAAGTCGGTG 
GCACTGCGCT CCTCGTCGAT TCCACTGCAG CAAATGTGGC TACCCCACAA GCCAGTGATG 
TTACTCCATG TTAAGGGGCG CACACATGTC CAAACCCGAC TGGTGGAACC CGTGCATACC 
TCCCTGAACC GTGGTGATTG CTTCATCCTG GTGGCCGGAT CGCAGCTTTT CCGCTACGTT 
GGATCCTTTG CAAACGTAAT CGAGATCTCC CGGAGCAAGA AGATCTGCGC TGCCATAGTG 
GAAAACAAGG ATCTCGGTTG CACCGCCACC CAGGAGGTGA TCCTCACGGA TGGCAAGTAT 
TTGAAGGAGC GCCAGTGGCG TCAGTTCTGG CAGCTTTTGG GCAAAAAAGA GGATGATCAA 
TCGGATATAG CTGAATGCGG TCATGCCGAT GAAGACGACG TGTTCGAGAG CAGCCTGATC 
GAGACTAACA AGATCTACGA GTACCAGGAT GAGGCGCTCA TTCCGTTGGA GAAATTCTGG 
GGCTGCATCC CCAAGGTGGA CATGTTGGAC ACGCGTAAAG TTTTGGTCTT TGACTTTGGC 
AGCGAGCTGT ATGTGTGGAA TGGCAAGAAT GCCCCTTCCG ATGCCAAGCG AGCTGCCATG 
AGATTGGCCC AGGAACACTT TGGTGCTGAG AATTCGGCGG ACTATGCCGA CTGTTACTTG 
AATCCCCTGA ACTATGTCGC CATAGTGGGT CGTCGTGAGA ACACCAAGTA TGCCAAGAGG 
TGCGCACAGC GTCCAGAATG GTGTGTTCTC GGCAAGATCA CCCAGAACAT GGAGACGGTG 
CTGTTCAAGG AGAAGTTCAG TGATTGGCCA GAGATAGAAC GCGAGGATCT GGAGAAGGAT 
TACCTATCAA ACGGAGTGCA TATGGTGCGT GCTTTAAACG GCACCGCTTT GCATAAGGGA 
GAACCCTACC AGGAACCAAA TCTGGTGCTC GAGCAGGCCA ACTTGGGGCG TGGCAATTTC 
TACTACGACA CGGACACCAT GCGGCACTTC GATGTGATTA CCAAGTCAAC GGACAAATGG 
CAGATCCACG AGTTCAACTT TGATGCGGCG AATAATCGCG ATGACTACGG TCACTTCTAC 
TCGGCGGAGA GCTATATAGT GCGCTGGATC TACCAGATTT CGGTCACAGT TCGCGAACTC 
AGTGGCAAGG TGTCGAACAG AAGCACAGTG GGTCGAGACA GGTGTGTCTA TTTCACCTGG 
CAAGGACAGG ACTCAAGTGC CAACGAGAAG GGTGCAGCTG CTTTGCTGAC CGTAGAGTTG 
GACAAGGAGA AAGGCGCACA GATGCGGGTG TCGCAAGGAG ATGAGTGCAC AGCCTTCGTC 
CGCCTGTTCC GGCAAATGTG GCAACATCGT GGACGCAAGG AGCAATGTCT GGAGAGGCAC 
AGCGAGTGGA GGCTCTACCA ACTGCAGGGC AACGTCAGCG AGGAGACCAT CATCAAGGAA 
GTCGTCTGCC AGTCCAGCCA TTTGCGATCC CGCAGCTCCA TGCTGTTAAT TCACGGCAGT 
CAGGGAAATG TGATTGTCTG GCATGGATCG AAGAGTGCTC CTCATACGCG TAATGTGGCT 
GTAGGAGTTG CTCAGGATCT GATCAAGACG GTGCCTAAAG ATCTCTTCAG CGCCGAAACT 
GCCAACCTAA CTGAGGTGGA GGAGGGTTCC GACGACGATC AGTGCAAGAA GGCCCTGGGA 
TTGAAGGATG AAAGAAATGA ATATGGCAGC CTGCTTAAGT CCACCAAGTC GTTCGATTAC 
CAAATAAGGA TCTTCAACTT CTCCAGCACT CAGGGTGTTT TCAAAGCCCT CGAACTGAGC 
GATCCGCTGC GCTGCCAGGA TCTGCATAGT CCGTATCCCT TCAGCCAGTC GCAGCTGTAC 
AATGCAAGGC AGCCGACTAT CTTTTTGCTG GACGACGGCG ATGAGTTGTG GCTCTGGATG 
GGATGGTGGC CTCTGGAAGA TGTGAAGATA AACACCGATG AGCGAAGCAG TCCCACCAAC 
GAAAATCGCG CTGGAGTAAA TCGCTGGATC TCAGAGAGAC GTGCTGCCCT CGAGACAGCC 
GTTGACTATT GGCGTGCCAA GCACGGCGAG AACGAGAATG AACCCTTCCA TGGCATCAAA 
GGCCAGGTTG TGTGGGCAGG CCTGGAGCCA CTGGCCTTTA AGGCGCTGTT TCCCGACTGG 
ACAGAACGTC CGGATGTAAG AGAAATCAAC GAAGAAGATG GGCGAACTGA TGCTTCCATA 
TCGATCGCCA CAATGCTGGC ACAAATGACG CAGACCGAGT ATCCCCTGGA AGTCCTGAAA 
GCCCGACCGC TGCCCGAAGG TGTGGAGCCC ACCCGCCTGG AGGTCTACCT TAATGCGGAG 
GACTTCCAGG CGGCACTGGG ATGCAGCCGG GCGGAATTCG AGCAGCTGCC CATCTGGAAA 
CAGACCAAGC TGAAGAAGGA GCGAGGGCTG TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Svil ( NM_001031933.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001027104.1 Drosophila melanogaster supervillin, transcript variant Y (Svil), mRNA.

Price & Availability↑

CloneID	OFa20086
Clone ID Related Accession (Same CDS sequence)	NM_001031933.3 , NM_206244.3
Accession Version	NM_001031933.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	supervillin, isoform Y
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001031933.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCCCCG ATCAGCGGTC TTTGGTGATT TCGAAGACCG GCTCCATTGC GGAGCGTTTG 
GCGGCACTCC ACAAAAGTGG CGAGGACGAC TGGAAGAAGC GCATCTCGAA GCGCGACGAA 
GTCGACGATG TGCACCGTGA AAACTTCGTA AACGAATCGC TATCTTTGGC CCACTCGCTG 
TCGGACAAAC CACTGCCCCC ATCACCTTTG ATACCCACCA GCTTGGAGGG CGGCAAGGTC 
TCCGATCGTT TGAGCAAACT CAAGTGCAAC TCGGAGAACT GGAAGCAGCG CATAGAGCAA 
ACCGATGCCA AGAAATTCAC AGTCGCCGGT CGACTGCAAA AGAAGGCCCA ATCCCCCGTG 
GAGCTTCAAT TCGAGCGTTC TGCCAATGAT GCGGCCAAAA AGTGTCCCAT GCTGGAGGTG 
CGCAGCGCCA ATCAGCCGCA GCTTGGGCTG GCCAAGAGCC CCTCCATGAT GGTCACCTCT 
AGCACCAGCA CCAATAGCAC CAGCAAGTCC GCACTCCGCA GCCTGAGCGT CAATCCCGAG 
GAGGAGGGAC CCACCAGCCT GAGTCGCTCC AACAGCAGCA GTTCGGAGTC CGATGGAGAG 
CGCAAATCCC CCCAGAATGC CAAGCAGAAC AAGGAGCTGG CCAACAACAA GAGTGCTGCA 
GCACCCACCA ATGTGGGAGC CAGGATTCAG GTGCCCCGGC TGGACGACAA GGAAACTTTC 
GAGAACTTCT TCTCCTCCAA GCCGAAGAAG GAGGAGAACG TTCAGCTGGA GATCAGCGCT 
TTCGATGACA TCAAGCCCAC TGAGCGCTTG GTAAGCAAGC GACACGTACA GGGTCCCAAA 
GGTCGACGGG CTGCACGCAA TCCTCTCAAG AGTTTAGCTG CTCGTGAGGA CATTAGCTCC 
GAGTACACCG AAATGAGGTC GGGCATAGCC GAGCGAGAAT TACGGCGCCT AAAGTTGGAG 
TCATATGGTC ATAATGCCAA CTTGGCTGCA GAGGCCATAG CCGGATTAGC CTCCGTCGAG 
GACTTCAAGT CGGTGGCACT GCGCTCCTCG TCGATTCCAC TGCAGCAAAT GTGGCTACCC 
CACAAGCCAG TGATGTTACT CCATGTTAAG GGGCGCACAC ATGTCCAAAC CCGACTGGTG 
GAACCCGTGC ATACCTCCCT GAACCGTGGT GATTGCTTCA TCCTGGTGGC CGGATCGCAG 
CTTTTCCGCT ACGTTGGATC CTTTGCAAAC GTAATCGAGA TCTCCCGGAG CAAGAAGATC 
TGCGCTGCCA TAGTGGAAAA CAAGGATCTC GGTTGCACCG CCACCCAGGA GGTGATCCTC 
ACGGATGGCA AGTATTTGAA GGAGCGCCAG TGGCGTCAGT TCTGGCAGCT TTTGGGCAAA 
AAAGAGGATG ATCAATCGGA TATAGCTGAA TGCGGTCATG CCGATGAAGA CGACGTGTTC 
GAGAGCAGCC TGATCGAGAC TAACAAGATC TACGAGTACC AGGATGAGGC GCTCATTCCG 
TTGGAGAAAT TCTGGGGCTG CATCCCCAAG GTGGACATGT TGGACACGCG TAAAGTTTTG 
GTCTTTGACT TTGGCAGCGA GCTGTATGTG TGGAATGGCA AGAATGCCCC TTCCGATGCC 
AAGCGAGCTG CCATGAGATT GGCCCAGGAA CACTTTGGTG CTGAGAATTC GGCGGACTAT 
GCCGACTGTT ACTTGAATCC CCTGAACTAT GTCGCCATAG TGGGTCGTCG TGAGAACACC 
AAGTATGCCA AGAGGTGCGC ACAGCGTCCA GAATGGTGTG TTCTCGGCAA GATCACCCAG 
AACATGGAGA CGGTGCTGTT CAAGGAGAAG TTCAGTGATT GGCCAGAGAT AGAACGCGAG 
GATCTGGAGA AGGATTACCT ATCAAACGGA GTGCATATGG TGCGTGCTTT AAACGGCACC 
GCTTTGCATA AGGGAGAACC CTACCAGGAA CCAAATCTGG TGCTCGAGCA GGCCAACTTG 
GGGCGTGGCA ATTTCTACTA CGACACGGAC ACCATGCGGC ACTTCGATGT GATTACCAAG 
TCAACGGACA AATGGCAGAT CCACGAGTTC AACTTTGATG CGGCGAATAA TCGCGATGAC 
TACGGTCACT TCTACTCGGC GGAGAGCTAT ATAGTGCGCT GGATCTACCA GATTTCGGTC 
ACAGTTCGCG AACTCAGTGG CAAGGTGTCG AACAGAAGCA CAGTGGGTCG AGACAGGTGT 
GTCTATTTCA CCTGGCAAGG ACAGGACTCA AGTGCCAACG AGAAGGGTGC AGCTGCTTTG 
CTGACCGTAG AGTTGGACAA GGAGAAAGGC GCACAGATGC GGGTGTCGCA AGGAGATGAG 
TGCACAGCCT TCGTCCGCCT GTTCCGGCAA ATGTGGCAAC ATCGTGGACG CAAGGAGCAA 
TGTCTGGAGA GGCACAGCGA GTGGAGGCTC TACCAACTGC AGGGCAACGT CAGCGAGGAG 
ACCATCATCA AGGAAGTCGT CTGCCAGTCC AGCCATTTGC GATCCCGCAG CTCCATGCTG 
TTAATTCACG GCAGTCAGGG AAATGTGATT GTCTGGCATG GATCGAAGAG TGCTCCTCAT 
ACGCGTAATG TGGCTGTAGG AGTTGCTCAG GATCTGATCA AGACGGTGCC TAAAGATCTC 
TTCAGCGCCG AAACTGCCAA CCTAACTGAG GTGGAGGAGG GTTCCGACGA CGATCAGTGC 
AAGAAGGCCC TGGGATTGAA GGATGAAAGA AATGAATATG GCAGCCTGCT TAAGTCCACC 
AAGTCGTTCG ATTACCAAAT AAGGATCTTC AACTTCTCCA GCACTCAGGG TGTTTTCAAA 
GCCCTCGAAC TGAGCGATCC GCTGCGCTGC CAGGATCTGC ATAGTCCGTA TCCCTTCAGC 
CAGTCGCAGC TGTACAATGC AAGGCAGCCG ACTATCTTTT TGCTGGACGA CGGCGATGAG 
TTGTGGCTCT GGATGGGATG GTGGCCTCTG GAAGATGTGA AGATAAACAC CGATGAGCGA 
AGCAGTCCCA CCAACGAAAA TCGCGCTGGA GTAAATCGCT GGATCTCAGA GAGACGTGCT 
GCCCTCGAGA CAGCCGTTGA CTATTGGCGT GCCAAGCACG GCGAGAACGA GAATGAACCC 
TTCCATGGCA TCAAAGGCCA GGTTGTGTGG GCAGGCCTGG AGCCACTGGC CTTTAAGGCG 
CTGTTTCCCG ACTGGACAGA ACGTCCGGAT GTAAGAGAAA TCAACGAAGA AGATGGGCGA 
ACTGATGCTT CCATATCGAT CGCCACAATG CTGGCACAAA TGACGCAGAC CGAGTATCCC 
CTGGAAGTCC TGAAAGCCCG ACCGCTGCCC GAAGGTGTGG AGCCCACCCG CCTGGAGGTC 
TACCTTAATG CGGAGGACTT CCAGGCGGCA CTGGGATGCA GCCGGGCGGA ATTCGAGCAG 
CTGCCCATCT GGAAACAGAC CAAGCTGAAG AAGGAGCGAG GGCTGTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001027104.1
CDS	687..4157
Translation	MGPDQRSLVISKTGSIAERLAALHKSGEDDWKKRISKRDEVDDVHRENFVNESLSLAHSLSDKPLPPSPLIPTSLEGGKVSDRLSKLKCNSENWKQRIEQTDAKKFTVAGRLQKKAQSPVELQFERSANDAAKKCPMLEVRSANQPQLGLAKSPSMMVTSSTSTNSTSKSALRSLSVNPEEEGPTSLSRSNSSSSESDGERKSPQNAKQNKELANNKSAAAPTNVGARIQVPRLDDKETFENFFSSKPKKEENVQLEISAFDDIKPTERLVSKRHVQGPKGRRAARNPLKSLAAREDISSEYTEMRSGIAERELRRLKLESYGHNANLAAEAIAGLASVEDFKSVALRSSSIPLQQMWLPHKPVMLLHVKGRTHVQTRLVEPVHTSLNRGDCFILVAGSQLFRYVGSFANVIEISRSKKICAAIVENKDLGCTATQEVILTDGKYLKERQWRQFWQLLGKKEDDQSDIAECGHADEDDVFESSLIETNKIYEYQDEALIPLEKFWGCIPKVDMLDTRKVLVFDFGSELYVWNGKNAPSDAKRAAMRLAQEHFGAENSADYADCYLNPLNYVAIVGRRENTKYAKRCAQRPEWCVLGKITQNMETVLFKEKFSDWPEIEREDLEKDYLSNGVHMVRALNGTALHKGEPYQEPNLVLEQANLGRGNFYYDTDTMRHFDVITKSTDKWQIHEFNFDAANNRDDYGHFYSAESYIVRWIYQISVTVRELSGKVSNRSTVGRDRCVYFTWQGQDSSANEKGAAALLTVELDKEKGAQMRVSQGDECTAFVRLFRQMWQHRGRKEQCLERHSEWRLYQLQGNVSEETIIKEVVCQSSHLRSRSSMLLIHGSQGNVIVWHGSKSAPHTRNVAVGVAQDLIKTVPKDLFSAETANLTEVEEGSDDDQCKKALGLKDERNEYGSLLKSTKSFDYQIRIFNFSSTQGVFKALELSDPLRCQDLHSPYPFSQSQLYNARQPTIFLLDDGDELWLWMGWWPLEDVKINTDERSSPTNENRAGVNRWISERRAALETAVDYWRAKHGENENEPFHGIKGQVVWAGLEPLAFKALFPDWTERPDVREINEEDGRTDASISIATMLAQMTQTEYPLEVLKARPLPEGVEPTRLEVYLNAEDFQAALGCSRAEFEQLPIWKQTKLKKERGLF

Target ORF information:

RefSeq Version	NM_001031933.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster supervillin, transcript variant Y (Svil), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001031933.3

ORF Insert Sequence:

ATGGGCCCCG ATCAGCGGTC TTTGGTGATT TCGAAGACCG GCTCCATTGC GGAGCGTTTG 
GCGGCACTCC ACAAAAGTGG CGAGGACGAC TGGAAGAAGC GCATCTCGAA GCGCGACGAA 
GTCGACGATG TGCACCGTGA AAACTTCGTA AACGAATCGC TATCTTTGGC CCACTCGCTG 
TCGGACAAAC CACTGCCCCC ATCACCTTTG ATACCCACCA GCTTGGAGGG CGGCAAGGTC 
TCCGATCGTT TGAGCAAACT CAAGTGCAAC TCGGAGAACT GGAAGCAGCG CATAGAGCAA 
ACCGATGCCA AGAAATTCAC AGTCGCCGGT CGACTGCAAA AGAAGGCCCA ATCCCCCGTG 
GAGCTTCAAT TCGAGCGTTC TGCCAATGAT GCGGCCAAAA AGTGTCCCAT GCTGGAGGTG 
CGCAGCGCCA ATCAGCCGCA GCTTGGGCTG GCCAAGAGCC CCTCCATGAT GGTCACCTCT 
AGCACCAGCA CCAATAGCAC CAGCAAGTCC GCACTCCGCA GCCTGAGCGT CAATCCCGAG 
GAGGAGGGAC CCACCAGCCT GAGTCGCTCC AACAGCAGCA GTTCGGAGTC CGATGGAGAG 
CGCAAATCCC CCCAGAATGC CAAGCAGAAC AAGGAGCTGG CCAACAACAA GAGTGCTGCA 
GCACCCACCA ATGTGGGAGC CAGGATTCAG GTGCCCCGGC TGGACGACAA GGAAACTTTC 
GAGAACTTCT TCTCCTCCAA GCCGAAGAAG GAGGAGAACG TTCAGCTGGA GATCAGCGCT 
TTCGATGACA TCAAGCCCAC TGAGCGCTTG GTAAGCAAGC GACACGTACA GGGTCCCAAA 
GGTCGACGGG CTGCACGCAA TCCTCTCAAG AGTTTAGCTG CTCGTGAGGA CATTAGCTCC 
GAGTACACCG AAATGAGGTC GGGCATAGCC GAGCGAGAAT TACGGCGCCT AAAGTTGGAG 
TCATATGGTC ATAATGCCAA CTTGGCTGCA GAGGCCATAG CCGGATTAGC CTCCGTCGAG 
GACTTCAAGT CGGTGGCACT GCGCTCCTCG TCGATTCCAC TGCAGCAAAT GTGGCTACCC 
CACAAGCCAG TGATGTTACT CCATGTTAAG GGGCGCACAC ATGTCCAAAC CCGACTGGTG 
GAACCCGTGC ATACCTCCCT GAACCGTGGT GATTGCTTCA TCCTGGTGGC CGGATCGCAG 
CTTTTCCGCT ACGTTGGATC CTTTGCAAAC GTAATCGAGA TCTCCCGGAG CAAGAAGATC 
TGCGCTGCCA TAGTGGAAAA CAAGGATCTC GGTTGCACCG CCACCCAGGA GGTGATCCTC 
ACGGATGGCA AGTATTTGAA GGAGCGCCAG TGGCGTCAGT TCTGGCAGCT TTTGGGCAAA 
AAAGAGGATG ATCAATCGGA TATAGCTGAA TGCGGTCATG CCGATGAAGA CGACGTGTTC 
GAGAGCAGCC TGATCGAGAC TAACAAGATC TACGAGTACC AGGATGAGGC GCTCATTCCG 
TTGGAGAAAT TCTGGGGCTG CATCCCCAAG GTGGACATGT TGGACACGCG TAAAGTTTTG 
GTCTTTGACT TTGGCAGCGA GCTGTATGTG TGGAATGGCA AGAATGCCCC TTCCGATGCC 
AAGCGAGCTG CCATGAGATT GGCCCAGGAA CACTTTGGTG CTGAGAATTC GGCGGACTAT 
GCCGACTGTT ACTTGAATCC CCTGAACTAT GTCGCCATAG TGGGTCGTCG TGAGAACACC 
AAGTATGCCA AGAGGTGCGC ACAGCGTCCA GAATGGTGTG TTCTCGGCAA GATCACCCAG 
AACATGGAGA CGGTGCTGTT CAAGGAGAAG TTCAGTGATT GGCCAGAGAT AGAACGCGAG 
GATCTGGAGA AGGATTACCT ATCAAACGGA GTGCATATGG TGCGTGCTTT AAACGGCACC 
GCTTTGCATA AGGGAGAACC CTACCAGGAA CCAAATCTGG TGCTCGAGCA GGCCAACTTG 
GGGCGTGGCA ATTTCTACTA CGACACGGAC ACCATGCGGC ACTTCGATGT GATTACCAAG 
TCAACGGACA AATGGCAGAT CCACGAGTTC AACTTTGATG CGGCGAATAA TCGCGATGAC 
TACGGTCACT TCTACTCGGC GGAGAGCTAT ATAGTGCGCT GGATCTACCA GATTTCGGTC 
ACAGTTCGCG AACTCAGTGG CAAGGTGTCG AACAGAAGCA CAGTGGGTCG AGACAGGTGT 
GTCTATTTCA CCTGGCAAGG ACAGGACTCA AGTGCCAACG AGAAGGGTGC AGCTGCTTTG 
CTGACCGTAG AGTTGGACAA GGAGAAAGGC GCACAGATGC GGGTGTCGCA AGGAGATGAG 
TGCACAGCCT TCGTCCGCCT GTTCCGGCAA ATGTGGCAAC ATCGTGGACG CAAGGAGCAA 
TGTCTGGAGA GGCACAGCGA GTGGAGGCTC TACCAACTGC AGGGCAACGT CAGCGAGGAG 
ACCATCATCA AGGAAGTCGT CTGCCAGTCC AGCCATTTGC GATCCCGCAG CTCCATGCTG 
TTAATTCACG GCAGTCAGGG AAATGTGATT GTCTGGCATG GATCGAAGAG TGCTCCTCAT 
ACGCGTAATG TGGCTGTAGG AGTTGCTCAG GATCTGATCA AGACGGTGCC TAAAGATCTC 
TTCAGCGCCG AAACTGCCAA CCTAACTGAG GTGGAGGAGG GTTCCGACGA CGATCAGTGC 
AAGAAGGCCC TGGGATTGAA GGATGAAAGA AATGAATATG GCAGCCTGCT TAAGTCCACC 
AAGTCGTTCG ATTACCAAAT AAGGATCTTC AACTTCTCCA GCACTCAGGG TGTTTTCAAA 
GCCCTCGAAC TGAGCGATCC GCTGCGCTGC CAGGATCTGC ATAGTCCGTA TCCCTTCAGC 
CAGTCGCAGC TGTACAATGC AAGGCAGCCG ACTATCTTTT TGCTGGACGA CGGCGATGAG 
TTGTGGCTCT GGATGGGATG GTGGCCTCTG GAAGATGTGA AGATAAACAC CGATGAGCGA 
AGCAGTCCCA CCAACGAAAA TCGCGCTGGA GTAAATCGCT GGATCTCAGA GAGACGTGCT 
GCCCTCGAGA CAGCCGTTGA CTATTGGCGT GCCAAGCACG GCGAGAACGA GAATGAACCC 
TTCCATGGCA TCAAAGGCCA GGTTGTGTGG GCAGGCCTGG AGCCACTGGC CTTTAAGGCG 
CTGTTTCCCG ACTGGACAGA ACGTCCGGAT GTAAGAGAAA TCAACGAAGA AGATGGGCGA 
ACTGATGCTT CCATATCGAT CGCCACAATG CTGGCACAAA TGACGCAGAC CGAGTATCCC 
CTGGAAGTCC TGAAAGCCCG ACCGCTGCCC GAAGGTGTGG AGCCCACCCG CCTGGAGGTC 
TACCTTAATG CGGAGGACTT CCAGGCGGCA CTGGGATGCA GCCGGGCGGA ATTCGAGCAG 
CTGCCCATCT GGAAACAGAC CAAGCTGAAG AAGGAGCGAG GGCTGTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Svil ( NM_206244.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995966.1 Drosophila melanogaster supervillin, transcript variant Z (Svil), mRNA.

Price & Availability↑

CloneID	OFa20086
Clone ID Related Accession (Same CDS sequence)	NM_001031933.3 , NM_206244.3
Accession Version	NM_206244.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	supervillin, isoform Z
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_206244.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCCCCG ATCAGCGGTC TTTGGTGATT TCGAAGACCG GCTCCATTGC GGAGCGTTTG 
GCGGCACTCC ACAAAAGTGG CGAGGACGAC TGGAAGAAGC GCATCTCGAA GCGCGACGAA 
GTCGACGATG TGCACCGTGA AAACTTCGTA AACGAATCGC TATCTTTGGC CCACTCGCTG 
TCGGACAAAC CACTGCCCCC ATCACCTTTG ATACCCACCA GCTTGGAGGG CGGCAAGGTC 
TCCGATCGTT TGAGCAAACT CAAGTGCAAC TCGGAGAACT GGAAGCAGCG CATAGAGCAA 
ACCGATGCCA AGAAATTCAC AGTCGCCGGT CGACTGCAAA AGAAGGCCCA ATCCCCCGTG 
GAGCTTCAAT TCGAGCGTTC TGCCAATGAT GCGGCCAAAA AGTGTCCCAT GCTGGAGGTG 
CGCAGCGCCA ATCAGCCGCA GCTTGGGCTG GCCAAGAGCC CCTCCATGAT GGTCACCTCT 
AGCACCAGCA CCAATAGCAC CAGCAAGTCC GCACTCCGCA GCCTGAGCGT CAATCCCGAG 
GAGGAGGGAC CCACCAGCCT GAGTCGCTCC AACAGCAGCA GTTCGGAGTC CGATGGAGAG 
CGCAAATCCC CCCAGAATGC CAAGCAGAAC AAGGAGCTGG CCAACAACAA GAGTGCTGCA 
GCACCCACCA ATGTGGGAGC CAGGATTCAG GTGCCCCGGC TGGACGACAA GGAAACTTTC 
GAGAACTTCT TCTCCTCCAA GCCGAAGAAG GAGGAGAACG TTCAGCTGGA GATCAGCGCT 
TTCGATGACA TCAAGCCCAC TGAGCGCTTG GTAAGCAAGC GACACGTACA GGGTCCCAAA 
GGTCGACGGG CTGCACGCAA TCCTCTCAAG AGTTTAGCTG CTCGTGAGGA CATTAGCTCC 
GAGTACACCG AAATGAGGTC GGGCATAGCC GAGCGAGAAT TACGGCGCCT AAAGTTGGAG 
TCATATGGTC ATAATGCCAA CTTGGCTGCA GAGGCCATAG CCGGATTAGC CTCCGTCGAG 
GACTTCAAGT CGGTGGCACT GCGCTCCTCG TCGATTCCAC TGCAGCAAAT GTGGCTACCC 
CACAAGCCAG TGATGTTACT CCATGTTAAG GGGCGCACAC ATGTCCAAAC CCGACTGGTG 
GAACCCGTGC ATACCTCCCT GAACCGTGGT GATTGCTTCA TCCTGGTGGC CGGATCGCAG 
CTTTTCCGCT ACGTTGGATC CTTTGCAAAC GTAATCGAGA TCTCCCGGAG CAAGAAGATC 
TGCGCTGCCA TAGTGGAAAA CAAGGATCTC GGTTGCACCG CCACCCAGGA GGTGATCCTC 
ACGGATGGCA AGTATTTGAA GGAGCGCCAG TGGCGTCAGT TCTGGCAGCT TTTGGGCAAA 
AAAGAGGATG ATCAATCGGA TATAGCTGAA TGCGGTCATG CCGATGAAGA CGACGTGTTC 
GAGAGCAGCC TGATCGAGAC TAACAAGATC TACGAGTACC AGGATGAGGC GCTCATTCCG 
TTGGAGAAAT TCTGGGGCTG CATCCCCAAG GTGGACATGT TGGACACGCG TAAAGTTTTG 
GTCTTTGACT TTGGCAGCGA GCTGTATGTG TGGAATGGCA AGAATGCCCC TTCCGATGCC 
AAGCGAGCTG CCATGAGATT GGCCCAGGAA CACTTTGGTG CTGAGAATTC GGCGGACTAT 
GCCGACTGTT ACTTGAATCC CCTGAACTAT GTCGCCATAG TGGGTCGTCG TGAGAACACC 
AAGTATGCCA AGAGGTGCGC ACAGCGTCCA GAATGGTGTG TTCTCGGCAA GATCACCCAG 
AACATGGAGA CGGTGCTGTT CAAGGAGAAG TTCAGTGATT GGCCAGAGAT AGAACGCGAG 
GATCTGGAGA AGGATTACCT ATCAAACGGA GTGCATATGG TGCGTGCTTT AAACGGCACC 
GCTTTGCATA AGGGAGAACC CTACCAGGAA CCAAATCTGG TGCTCGAGCA GGCCAACTTG 
GGGCGTGGCA ATTTCTACTA CGACACGGAC ACCATGCGGC ACTTCGATGT GATTACCAAG 
TCAACGGACA AATGGCAGAT CCACGAGTTC AACTTTGATG CGGCGAATAA TCGCGATGAC 
TACGGTCACT TCTACTCGGC GGAGAGCTAT ATAGTGCGCT GGATCTACCA GATTTCGGTC 
ACAGTTCGCG AACTCAGTGG CAAGGTGTCG AACAGAAGCA CAGTGGGTCG AGACAGGTGT 
GTCTATTTCA CCTGGCAAGG ACAGGACTCA AGTGCCAACG AGAAGGGTGC AGCTGCTTTG 
CTGACCGTAG AGTTGGACAA GGAGAAAGGC GCACAGATGC GGGTGTCGCA AGGAGATGAG 
TGCACAGCCT TCGTCCGCCT GTTCCGGCAA ATGTGGCAAC ATCGTGGACG CAAGGAGCAA 
TGTCTGGAGA GGCACAGCGA GTGGAGGCTC TACCAACTGC AGGGCAACGT CAGCGAGGAG 
ACCATCATCA AGGAAGTCGT CTGCCAGTCC AGCCATTTGC GATCCCGCAG CTCCATGCTG 
TTAATTCACG GCAGTCAGGG AAATGTGATT GTCTGGCATG GATCGAAGAG TGCTCCTCAT 
ACGCGTAATG TGGCTGTAGG AGTTGCTCAG GATCTGATCA AGACGGTGCC TAAAGATCTC 
TTCAGCGCCG AAACTGCCAA CCTAACTGAG GTGGAGGAGG GTTCCGACGA CGATCAGTGC 
AAGAAGGCCC TGGGATTGAA GGATGAAAGA AATGAATATG GCAGCCTGCT TAAGTCCACC 
AAGTCGTTCG ATTACCAAAT AAGGATCTTC AACTTCTCCA GCACTCAGGG TGTTTTCAAA 
GCCCTCGAAC TGAGCGATCC GCTGCGCTGC CAGGATCTGC ATAGTCCGTA TCCCTTCAGC 
CAGTCGCAGC TGTACAATGC AAGGCAGCCG ACTATCTTTT TGCTGGACGA CGGCGATGAG 
TTGTGGCTCT GGATGGGATG GTGGCCTCTG GAAGATGTGA AGATAAACAC CGATGAGCGA 
AGCAGTCCCA CCAACGAAAA TCGCGCTGGA GTAAATCGCT GGATCTCAGA GAGACGTGCT 
GCCCTCGAGA CAGCCGTTGA CTATTGGCGT GCCAAGCACG GCGAGAACGA GAATGAACCC 
TTCCATGGCA TCAAAGGCCA GGTTGTGTGG GCAGGCCTGG AGCCACTGGC CTTTAAGGCG 
CTGTTTCCCG ACTGGACAGA ACGTCCGGAT GTAAGAGAAA TCAACGAAGA AGATGGGCGA 
ACTGATGCTT CCATATCGAT CGCCACAATG CTGGCACAAA TGACGCAGAC CGAGTATCCC 
CTGGAAGTCC TGAAAGCCCG ACCGCTGCCC GAAGGTGTGG AGCCCACCCG CCTGGAGGTC 
TACCTTAATG CGGAGGACTT CCAGGCGGCA CTGGGATGCA GCCGGGCGGA ATTCGAGCAG 
CTGCCCATCT GGAAACAGAC CAAGCTGAAG AAGGAGCGAG GGCTGTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995966.1
CDS	396..3866
Translation	MGPDQRSLVISKTGSIAERLAALHKSGEDDWKKRISKRDEVDDVHRENFVNESLSLAHSLSDKPLPPSPLIPTSLEGGKVSDRLSKLKCNSENWKQRIEQTDAKKFTVAGRLQKKAQSPVELQFERSANDAAKKCPMLEVRSANQPQLGLAKSPSMMVTSSTSTNSTSKSALRSLSVNPEEEGPTSLSRSNSSSSESDGERKSPQNAKQNKELANNKSAAAPTNVGARIQVPRLDDKETFENFFSSKPKKEENVQLEISAFDDIKPTERLVSKRHVQGPKGRRAARNPLKSLAAREDISSEYTEMRSGIAERELRRLKLESYGHNANLAAEAIAGLASVEDFKSVALRSSSIPLQQMWLPHKPVMLLHVKGRTHVQTRLVEPVHTSLNRGDCFILVAGSQLFRYVGSFANVIEISRSKKICAAIVENKDLGCTATQEVILTDGKYLKERQWRQFWQLLGKKEDDQSDIAECGHADEDDVFESSLIETNKIYEYQDEALIPLEKFWGCIPKVDMLDTRKVLVFDFGSELYVWNGKNAPSDAKRAAMRLAQEHFGAENSADYADCYLNPLNYVAIVGRRENTKYAKRCAQRPEWCVLGKITQNMETVLFKEKFSDWPEIEREDLEKDYLSNGVHMVRALNGTALHKGEPYQEPNLVLEQANLGRGNFYYDTDTMRHFDVITKSTDKWQIHEFNFDAANNRDDYGHFYSAESYIVRWIYQISVTVRELSGKVSNRSTVGRDRCVYFTWQGQDSSANEKGAAALLTVELDKEKGAQMRVSQGDECTAFVRLFRQMWQHRGRKEQCLERHSEWRLYQLQGNVSEETIIKEVVCQSSHLRSRSSMLLIHGSQGNVIVWHGSKSAPHTRNVAVGVAQDLIKTVPKDLFSAETANLTEVEEGSDDDQCKKALGLKDERNEYGSLLKSTKSFDYQIRIFNFSSTQGVFKALELSDPLRCQDLHSPYPFSQSQLYNARQPTIFLLDDGDELWLWMGWWPLEDVKINTDERSSPTNENRAGVNRWISERRAALETAVDYWRAKHGENENEPFHGIKGQVVWAGLEPLAFKALFPDWTERPDVREINEEDGRTDASISIATMLAQMTQTEYPLEVLKARPLPEGVEPTRLEVYLNAEDFQAALGCSRAEFEQLPIWKQTKLKKERGLF

Target ORF information:

RefSeq Version	NM_206244.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster supervillin, transcript variant Z (Svil), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206244.3

ORF Insert Sequence:

ATGGGCCCCG ATCAGCGGTC TTTGGTGATT TCGAAGACCG GCTCCATTGC GGAGCGTTTG 
GCGGCACTCC ACAAAAGTGG CGAGGACGAC TGGAAGAAGC GCATCTCGAA GCGCGACGAA 
GTCGACGATG TGCACCGTGA AAACTTCGTA AACGAATCGC TATCTTTGGC CCACTCGCTG 
TCGGACAAAC CACTGCCCCC ATCACCTTTG ATACCCACCA GCTTGGAGGG CGGCAAGGTC 
TCCGATCGTT TGAGCAAACT CAAGTGCAAC TCGGAGAACT GGAAGCAGCG CATAGAGCAA 
ACCGATGCCA AGAAATTCAC AGTCGCCGGT CGACTGCAAA AGAAGGCCCA ATCCCCCGTG 
GAGCTTCAAT TCGAGCGTTC TGCCAATGAT GCGGCCAAAA AGTGTCCCAT GCTGGAGGTG 
CGCAGCGCCA ATCAGCCGCA GCTTGGGCTG GCCAAGAGCC CCTCCATGAT GGTCACCTCT 
AGCACCAGCA CCAATAGCAC CAGCAAGTCC GCACTCCGCA GCCTGAGCGT CAATCCCGAG 
GAGGAGGGAC CCACCAGCCT GAGTCGCTCC AACAGCAGCA GTTCGGAGTC CGATGGAGAG 
CGCAAATCCC CCCAGAATGC CAAGCAGAAC AAGGAGCTGG CCAACAACAA GAGTGCTGCA 
GCACCCACCA ATGTGGGAGC CAGGATTCAG GTGCCCCGGC TGGACGACAA GGAAACTTTC 
GAGAACTTCT TCTCCTCCAA GCCGAAGAAG GAGGAGAACG TTCAGCTGGA GATCAGCGCT 
TTCGATGACA TCAAGCCCAC TGAGCGCTTG GTAAGCAAGC GACACGTACA GGGTCCCAAA 
GGTCGACGGG CTGCACGCAA TCCTCTCAAG AGTTTAGCTG CTCGTGAGGA CATTAGCTCC 
GAGTACACCG AAATGAGGTC GGGCATAGCC GAGCGAGAAT TACGGCGCCT AAAGTTGGAG 
TCATATGGTC ATAATGCCAA CTTGGCTGCA GAGGCCATAG CCGGATTAGC CTCCGTCGAG 
GACTTCAAGT CGGTGGCACT GCGCTCCTCG TCGATTCCAC TGCAGCAAAT GTGGCTACCC 
CACAAGCCAG TGATGTTACT CCATGTTAAG GGGCGCACAC ATGTCCAAAC CCGACTGGTG 
GAACCCGTGC ATACCTCCCT GAACCGTGGT GATTGCTTCA TCCTGGTGGC CGGATCGCAG 
CTTTTCCGCT ACGTTGGATC CTTTGCAAAC GTAATCGAGA TCTCCCGGAG CAAGAAGATC 
TGCGCTGCCA TAGTGGAAAA CAAGGATCTC GGTTGCACCG CCACCCAGGA GGTGATCCTC 
ACGGATGGCA AGTATTTGAA GGAGCGCCAG TGGCGTCAGT TCTGGCAGCT TTTGGGCAAA 
AAAGAGGATG ATCAATCGGA TATAGCTGAA TGCGGTCATG CCGATGAAGA CGACGTGTTC 
GAGAGCAGCC TGATCGAGAC TAACAAGATC TACGAGTACC AGGATGAGGC GCTCATTCCG 
TTGGAGAAAT TCTGGGGCTG CATCCCCAAG GTGGACATGT TGGACACGCG TAAAGTTTTG 
GTCTTTGACT TTGGCAGCGA GCTGTATGTG TGGAATGGCA AGAATGCCCC TTCCGATGCC 
AAGCGAGCTG CCATGAGATT GGCCCAGGAA CACTTTGGTG CTGAGAATTC GGCGGACTAT 
GCCGACTGTT ACTTGAATCC CCTGAACTAT GTCGCCATAG TGGGTCGTCG TGAGAACACC 
AAGTATGCCA AGAGGTGCGC ACAGCGTCCA GAATGGTGTG TTCTCGGCAA GATCACCCAG 
AACATGGAGA CGGTGCTGTT CAAGGAGAAG TTCAGTGATT GGCCAGAGAT AGAACGCGAG 
GATCTGGAGA AGGATTACCT ATCAAACGGA GTGCATATGG TGCGTGCTTT AAACGGCACC 
GCTTTGCATA AGGGAGAACC CTACCAGGAA CCAAATCTGG TGCTCGAGCA GGCCAACTTG 
GGGCGTGGCA ATTTCTACTA CGACACGGAC ACCATGCGGC ACTTCGATGT GATTACCAAG 
TCAACGGACA AATGGCAGAT CCACGAGTTC AACTTTGATG CGGCGAATAA TCGCGATGAC 
TACGGTCACT TCTACTCGGC GGAGAGCTAT ATAGTGCGCT GGATCTACCA GATTTCGGTC 
ACAGTTCGCG AACTCAGTGG CAAGGTGTCG AACAGAAGCA CAGTGGGTCG AGACAGGTGT 
GTCTATTTCA CCTGGCAAGG ACAGGACTCA AGTGCCAACG AGAAGGGTGC AGCTGCTTTG 
CTGACCGTAG AGTTGGACAA GGAGAAAGGC GCACAGATGC GGGTGTCGCA AGGAGATGAG 
TGCACAGCCT TCGTCCGCCT GTTCCGGCAA ATGTGGCAAC ATCGTGGACG CAAGGAGCAA 
TGTCTGGAGA GGCACAGCGA GTGGAGGCTC TACCAACTGC AGGGCAACGT CAGCGAGGAG 
ACCATCATCA AGGAAGTCGT CTGCCAGTCC AGCCATTTGC GATCCCGCAG CTCCATGCTG 
TTAATTCACG GCAGTCAGGG AAATGTGATT GTCTGGCATG GATCGAAGAG TGCTCCTCAT 
ACGCGTAATG TGGCTGTAGG AGTTGCTCAG GATCTGATCA AGACGGTGCC TAAAGATCTC 
TTCAGCGCCG AAACTGCCAA CCTAACTGAG GTGGAGGAGG GTTCCGACGA CGATCAGTGC 
AAGAAGGCCC TGGGATTGAA GGATGAAAGA AATGAATATG GCAGCCTGCT TAAGTCCACC 
AAGTCGTTCG ATTACCAAAT AAGGATCTTC AACTTCTCCA GCACTCAGGG TGTTTTCAAA 
GCCCTCGAAC TGAGCGATCC GCTGCGCTGC CAGGATCTGC ATAGTCCGTA TCCCTTCAGC 
CAGTCGCAGC TGTACAATGC AAGGCAGCCG ACTATCTTTT TGCTGGACGA CGGCGATGAG 
TTGTGGCTCT GGATGGGATG GTGGCCTCTG GAAGATGTGA AGATAAACAC CGATGAGCGA 
AGCAGTCCCA CCAACGAAAA TCGCGCTGGA GTAAATCGCT GGATCTCAGA GAGACGTGCT 
GCCCTCGAGA CAGCCGTTGA CTATTGGCGT GCCAAGCACG GCGAGAACGA GAATGAACCC 
TTCCATGGCA TCAAAGGCCA GGTTGTGTGG GCAGGCCTGG AGCCACTGGC CTTTAAGGCG 
CTGTTTCCCG ACTGGACAGA ACGTCCGGAT GTAAGAGAAA TCAACGAAGA AGATGGGCGA 
ACTGATGCTT CCATATCGAT CGCCACAATG CTGGCACAAA TGACGCAGAC CGAGTATCCC 
CTGGAAGTCC TGAAAGCCCG ACCGCTGCCC GAAGGTGTGG AGCCCACCCG CCTGGAGGTC 
TACCTTAATG CGGAGGACTT CCAGGCGGCA CTGGGATGCA GCCGGGCGGA ATTCGAGCAG 
CTGCCCATCT GGAAACAGAC CAAGCTGAAG AAGGAGCGAG GGCTGTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Svil ( NM_139462.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_647719.2 Drosophila melanogaster supervillin, transcript variant AF (Svil), mRNA.

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CloneID	OFa17294
Clone ID Related Accession (Same CDS sequence)	NM_139462.4
Accession Version	NM_139462.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5802bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	supervillin, isoform AF
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_139462.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTATCTCA TACAGACTGT TCAAACCACT TGGCGGCCAC AATTGCCGCT GCCCAGGGAG 
CTTCAGCTGA CCAATGACCG GAATAGCGCC GACGACCACC ACGATTCAAA GGTGATAACG 
ACCACCACAA CCACAGCGGA CATCCATCAC CACAACCAGC AGGAGGAGAA CGAGGAAGAG 
GATATGGATC GGCTAAGGGG CAATCACAGC ATTGGAGGAT CCTCGCAGCA GCTTTCTGGC 
AAACAGAAAT GGTCCTTCGG GCGACTTTTT CGCAAGAAAA AGGAGGCGGA GAGTGCATCC 
TCCAGCGAAG AAGATCGCAA GGCGGGATTT GTGCCTGCCC AGCGCCAGTC AGCCAGCAAG 
CCGAAGGGAA AACATGGCAA AGGATCGCGA GGTTGCAGCA AATTCGATCA CATTGTGGTG 
AGTCAGCGGC AAGAGCAGGC TCCACCACAT CCACCCCAAC CGCAAATCGA CAATGAGTTC 
TTCTTGCCCT TGGAGGCCAT ACAACCGGAG CCCTACTATC AAAATCAACC GGGCTACTAT 
CAACGATCGA GTAACTCGCT GGACAGGAGA TTACTAATGC AGCCTGGAGT TCAGGGAAAT 
GGTTACCAGC AGCCCAAATC TCGATCGGCG CAGCGAGGTC CCAAGCCACC AGGAGCTCAC 
TCCAGCGAGG AGGAACTGAT CTCGCTGAAC TCCTCCACGT TCTCCAAATA CCGCAGCGAT 
GAGAGTATTC ATTTGGGTTC CGCTGCTGGA CAAAGTCGAC GCAGTCGAGC GGCCCGCAAT 
GAGCGTTACT ACAAGAGATT GTCCCGGGAT GGCGAACCGA GTCATGGCGG AGCACCCGTT 
GTAGCCCAGC AGCGATACAA AACCCAGCCA CTGCCTTTAT CCATTTACCA GCCAACGAAC 
GCGTCCACTG GCTATGTCCA GTGGCAACCG CAGCCCCAGA AGAACCTGCA GAATGCGATG 
CAGAACGATG GAAAGCGAAG CATCAGCTAC GACAGTCAAA TACACCTGCA GAACATGAAT 
GGGAGGATGC AGAGTAAGCC ATTGCCACCG CCACCGCCTC CAAGGGATCC ACTGAGGAGA 
GTGCATGTTG GAGGTTCCGG GAAGATTGGA TCCAGCTCTG GAGACTTGCG TCCCGTGTCC 
TATGCCTTCG ATCACAATAG TCGCTGTGCG TCGGATGATC GCATTTGGCA GCCACCCCAC 
TACCAGTCGG TGCATTCGCT CAACTCCCAG CCCAGTTCGT CAATAGTCAG TGCACCAGTG 
AGTCAACAGC AACCGCACCA CAGGAGATTC ATAACTCGAT CCGAGAGGAA CGCAAATCCT 
GGCAAGCAAT CCCTGCCGAA TGGTATTGAT TTCCATTATG TGGCAGATGC AACGCCACGC 
TCCAGGAAGC CCATACACAT GATGGATGCA GGCAGGATGG ACAATACAGG AGTGCCAGCA 
TCCAGTGGTA TATTGCGAAC CTCTAAGAGT GGGCTGCCCA CACCAAGTCC TGCTCCCCAG 
CAGCCCTTGG CCAAACCGCG GTCGTTCTCC AGCTCGCGAC TTAGTGAGCT GCGAACCTAT 
CCCATGCCCA TGTACTCCGA GGTGCAGAAA CCTAAGAGGA ATGCGCCACC AGTCAATCCC 
TCGCCGAATC AGGATAACAT AGTGTGTGGG AGTCTGCACA TAAAACCCAC ACAAGAGCAT 
GGAAATCAAA ACTATGTGGA GATTCGTCAT CGGGATGTGC ACAAGACCAA CTCAATGCCG 
CACCATTATC AGAATAGGCG ATCTGGCGAG GATTTACCCA ACAAATATGA GGAGTACGTG 
GCAGAAAAGA GGGAGCAGCA TCAACAGGCA CCACCCCCAG TTTATCCGGA GCGCAAAATG 
AGCTTGACAC CAGCAGTGGC GCCACCGCCT CCTGTTTACT TCCCTCGCAA GAAGCCTGCC 
AATTTGGAGG AGGCCATCAA CGAACTGGAG GCCATTTACA AGTCTCTAGG ACTCACTGAA 
CCAGCCGAGC CCAAACAGGA ACCAAAGCTG GATATTTCCT CCAAGATGCG TGTGCCAACT 
CCCAGTGATT TTGAAAAGTT CGCCTTGGCG CATGCTGATG ACTACGAGGA TGAAGATTCG 
CCAACCGGAG AACCAGATCC AATCAGAGAT GATGTGGCCT TCAGGAACCT ACAGTTGGCC 
AATCTGCAAC ATCGGCCATC GGAGAAGCAG CCTCCATTCG GCATCCCAGT GGGACCCATT 
GTGCCAGCTC CACAGTCGGA TTATCTTCAC GTAGAGCCAG TGAGGGTGAA GAAGAAGAGT 
GGCTCTCCTG ACATCATTAA AGATGATCTT GCTGTGCGTT CATTGAGAAA GGATCCTCCT 
GGTCCCAAAT CCAGCTTTGT CTACCCAATG CAAAAGAAGC AACGGGCTAT TCGAACCCAA 
TCGGCCAACA TATACAATCT GATCCAAAGA GACGCTGCCA AGCCATCTGG TGGAGATCTT 
AAGAGCTACA TGGAGCTGAC TCGCAACCTG GAAAGAGCTG GCAGCATGTC CAATCTGCAG 
GGTGAGGATG GCAGTGATAC CAAAGATGTA CCCGCCACAC TGGATCTTCT CCGAAAGTTG 
AAAGCTCAGG ATGAGGAACT GGATTTGGCT AGAAAGCATC ACCCCATACC GTTTAGACAT 
CCCAGTCAGG GAGGAGCTAT TGCCCAACTG CCAGAACGGT TGTCCAAAGA GGAAATACCC 
AAGGCAGCGC CTGTGCCAGC TCCCCGAAAG AGTCTGACTC CTGAACCCAT GCTAGATGAT 
GCTCTCAACA AGATTGCCCA GGATGCGCAG GCCAGCAGCA TTAAGCTGAG TCAGGAACTC 
CATGAACTTC GCAAGGAGGC ATTGATTACA GCTGCGAGAC CAAAGTTGAA TGCCGAACAA 
CAGAGATTAG AGGATGAACT GCAGCAGATA GAAGCTGTCT CCGAGGCAGC CAAACGTTGT 
GGCCAAATGC TGCTCGAAAC TCTGCCAGAT GCGGGACAGG AGGGTCAGGA ACCTCAGGAT 
AAGCCCCTCA GGAAGCTACA TAAGGAGGGC AAACTCATAC GAGCTATCGA TGAGGTGTCC 
GAAGCTGCTA ATGCGGTCTG TGAGAAGATC CTCAAGGACA TTGTGACCAC AGAACCACCG 
GTGGTAGTTC AGGAGGCCCT CCAAGTGAGG CAGACCACAA AGACTGGACC AGGCGGAGAA 
CAGCTGGTGA TGCCGCATCT TATCAAGAAA CTAGATCCCA TACAATCCGA CAAGATCGAG 
CACATTGCCA GACGATGCAT GCGGCAGTTG AGCGAACTGG CCAATAATCC GGACTACGAC 
AACCTGGCCA CCTGTAATAT GAGCAACAGC ACCACCAACG CAACGGGTGG AGCAGCCGCT 
GATGCCACTC TCGTTTGTCC CGTTCCAGAG ACCCAAGCCA AGAGCCAGAC GCTCGAGGAG 
ATTGACAAGA TCATGCAGGA GTGCGAGCGT CAGGCGAAGA GCAGCAGCTC AACCACGACC 
ACCGATGATC AGCGCACCAC TGCCCCCAGT CTGACGAGTG GAAGTGGCAG CGGGAGCTTC 
CCACCCAATG TGAGCACCAC CGCCAGTTCG TTCAGCTCAA GCAGCGACTG TTTGGCCAAG 
TCGTCGAGTC CTTCGCGCGC CAGCCGTCCG CTCTCCTCGA GCATCACCTC TTTCAATCCA 
TATTCCTCCA GCGACTACAT CAAGTCGCAG AGCAGTGATT GCCATGCACC AAGCACCGAT 
CCGATCAAGA CCTTTAGCAC CACCTCGTAC GAGGTGCAGT CCACACCGCA GAGCACCACC 
ATTAGCACCA ATACGATGAG CACGAGCCAG AGCAACACGA TCAGTAGCAC TACTAATCCG 
AGTGTGGAGT CCAAGCCATC CGCCCCGTCG GTATCGCCAC CACCCCTCCA GTTGACGCCA 
GTGGGTGATC GGAGGGGCAG GAACCGGGAG CGGGGAGCTT CCTGCTCCTC CACATCCTCA 
CCTTCACAGT ACAACTCGAG CGAGGAGCTG GCCGCCATTT TTGGCATCGA GGAGCAACCG 
AAACAGCAGC GTCGCAGTCG GCAGACGCAT AATGCCACAG GTAACGGAGA TGATCCCGCC 
ACCACTGAGG CCCCGAGTTC GCCTGATTCC GATTCCGCCA ACGCCAATGA TACTGCCAAT 
GCCAATGCCA ATACTACTAA TCCCGCCCAG CTCGATTCAG CTAATTGCGC TACTAATCCC 
GTTATGAATG TTCAACTTGA TGTTACTACT AGCACCTCCA CCTCAGCCTC AACCACCCAT 
CCAACCTCTA ACGATAATGC TAGCACTTGT AGTAGCCATA GTTATAAGCT AGAGACGATT 
CCGGAGCAGG ATCACGAGGA GGTGGAGTCC ACGCTAAAGT TGTCGCCGAA CTTCAATAGG 
ATTTATGCCA GCAAGATTCG CATAGTAATG GATCGCAATG AAGAGGCAAG TCTCCTGCCG 
GAAGGAGCAG CTAATGTTGC TTTAACTCAA TTCGATTCTT TGAGTTCTCT GGAAGAGACC 
TCGTCCAATC CGGACTCTGG GAATGTTAGT CTCACGGATT ATCCCCTGGC TAACCATGCG 
AATTCCTCCC TGGAACTGCA GACCGCTTAC AATAGTCGAT TTCAAGTGAG GGAGCATTAC 
GTATCCGAGG TTTCCATTAG TGCTTCGGAA TCAACGGATG AGGAGCAGAC GGACACGGAG 
TGCTTAAGAG ATGCTGATCG TTTGAACACA GAAGACTTTC CACCTTTACC CACAGAAGAA 
GAGCTAGAGG AGAGTCTACG GGAAGAAATA GAACTTAACG ATAGTCTTAG TGGGGGCACT 
TTAAATATTG CTAGAGAGAG TAGCGGAGAA TTGGTATCTG TAGTTCAAGA ATTGAACCTG 
AATGAAACTG TTAGTCTGAG TCAAGAGGTT TACAATGTAA CCACTGAGGC GCTGCAACGA 
ATAGCGACAG ATGAAATAGA TGGCTTAAGT TCCGCTATAA TTGAGGTTAA CGAAAGCTTA 
CTTAACGAAT CTGCATTTGA TGAGATGCAC TTTAAACTAG ACGAAACGGA ACTGGACGAA 
TCGGAAATCA TAGACAGAAT GCTTGAGGCT TCATTTGACG AGTTAGACGA ATTAGAACTT 
GAGAACACAG AACTTGACGA AAGTCGACTT GACGACGAAC AAAAGAGCGA ATGCCAATTT 
AACGAAACCT ATAACGAGTT GGACAGCATA GATCTTGATG CCACAGAACT TAACGCCACT 
GGACTTAACG AAATTTCATA TGACCAGCTG GAGGAACTAG AACTTGACGA TAGCGACTCC 
GTAATGCCGT TCACACGGCG CAAGAGCATC GTTTACGAAA CGCCACAACT TAACCTGAGC 
ATTTCCGAAC TAAAAGTGAA TTACGAAACG CAAGTGGAGC CAGTAATTAA ATCAGAGTCA 
CTCAACGAAG CCATTAAATC GATGAACAAC GACGATGAAG GCGACGAGGA GATGCAGGCA 
GGCAGTGAAG AGAAGGTAGA TGAAGAGGAA AACAGTGTAA CTGAGACCCA GACACATCGT 
CGTCGGTGCA AAGGACTTCC GGCGAATCCC GCTAATGATG ATGAACGGCC GTCCACCAGC 
TGCAAGGCTC TTTCCGTTTT TGGCGAGGTG CGAACTGTGG CACCACAGGC GGCGGCGGCA 
GAGGCAGCTG CAGCAGTTGG TGGTGCAACG CGGGGCGGTT GGACTACACT CGAGCACGTA 
TTTGTCGCCT GCACTGTGGG CCTGATTACA CCGAACGATT TGCTCACTTT GTGCCTGATC 
GTAATTGGTG TGATTATCAT TATTGCAATT GCTCTCACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_647719.2
CDS	776..6577
Translation	MYLIQTVQTTWRPQLPLPRELQLTNDRNSADDHHDSKVITTTTTTADIHHHNQQEENEEEDMDRLRGNHSIGGSSQQLSGKQKWSFGRLFRKKKEAESASSSEEDRKAGFVPAQRQSASKPKGKHGKGSRGCSKFDHIVVSQRQEQAPPHPPQPQIDNEFFLPLEAIQPEPYYQNQPGYYQRSSNSLDRRLLMQPGVQGNGYQQPKSRSAQRGPKPPGAHSSEEELISLNSSTFSKYRSDESIHLGSAAGQSRRSRAARNERYYKRLSRDGEPSHGGAPVVAQQRYKTQPLPLSIYQPTNASTGYVQWQPQPQKNLQNAMQNDGKRSISYDSQIHLQNMNGRMQSKPLPPPPPPRDPLRRVHVGGSGKIGSSSGDLRPVSYAFDHNSRCASDDRIWQPPHYQSVHSLNSQPSSSIVSAPVSQQQPHHRRFITRSERNANPGKQSLPNGIDFHYVADATPRSRKPIHMMDAGRMDNTGVPASSGILRTSKSGLPTPSPAPQQPLAKPRSFSSSRLSELRTYPMPMYSEVQKPKRNAPPVNPSPNQDNIVCGSLHIKPTQEHGNQNYVEIRHRDVHKTNSMPHHYQNRRSGEDLPNKYEEYVAEKREQHQQAPPPVYPERKMSLTPAVAPPPPVYFPRKKPANLEEAINELEAIYKSLGLTEPAEPKQEPKLDISSKMRVPTPSDFEKFALAHADDYEDEDSPTGEPDPIRDDVAFRNLQLANLQHRPSEKQPPFGIPVGPIVPAPQSDYLHVEPVRVKKKSGSPDIIKDDLAVRSLRKDPPGPKSSFVYPMQKKQRAIRTQSANIYNLIQRDAAKPSGGDLKSYMELTRNLERAGSMSNLQGEDGSDTKDVPATLDLLRKLKAQDEELDLARKHHPIPFRHPSQGGAIAQLPERLSKEEIPKAAPVPAPRKSLTPEPMLDDALNKIAQDAQASSIKLSQELHELRKEALITAARPKLNAEQQRLEDELQQIEAVSEAAKRCGQMLLETLPDAGQEGQEPQDKPLRKLHKEGKLIRAIDEVSEAANAVCEKILKDIVTTEPPVVVQEALQVRQTTKTGPGGEQLVMPHLIKKLDPIQSDKIEHIARRCMRQLSELANNPDYDNLATCNMSNSTTNATGGAAADATLVCPVPETQAKSQTLEEIDKIMQECERQAKSSSSTTTTDDQRTTAPSLTSGSGSGSFPPNVSTTASSFSSSSDCLAKSSSPSRASRPLSSSITSFNPYSSSDYIKSQSSDCHAPSTDPIKTFSTTSYEVQSTPQSTTISTNTMSTSQSNTISSTTNPSVESKPSAPSVSPPPLQLTPVGDRRGRNRERGASCSSTSSPSQYNSSEELAAIFGIEEQPKQQRRSRQTHNATGNGDDPATTEAPSSPDSDSANANDTANANANTTNPAQLDSANCATNPVMNVQLDVTTSTSTSASTTHPTSNDNASTCSSHSYKLETIPEQDHEEVESTLKLSPNFNRIYASKIRIVMDRNEEASLLPEGAANVALTQFDSLSSLEETSSNPDSGNVSLTDYPLANHANSSLELQTAYNSRFQVREHYVSEVSISASESTDEEQTDTECLRDADRLNTEDFPPLPTEEELEESLREEIELNDSLSGGTLNIARESSGELVSVVQELNLNETVSLSQEVYNVTTEALQRIATDEIDGLSSAIIEVNESLLNESAFDEMHFKLDETELDESEIIDRMLEASFDELDELELENTELDESRLDDEQKSECQFNETYNELDSIDLDATELNATGLNEISYDQLEELELDDSDSVMPFTRRKSIVYETPQLNLSISELKVNYETQVEPVIKSESLNEAIKSMNNDDEGDEEMQAGSEEKVDEEENSVTETQTHRRRCKGLPANPANDDERPSTSCKALSVFGEVRTVAPQAAAAEAAAAVGGATRGGWTTLEHVFVACTVGLITPNDLLTLCLIVIGVIIIIAIALT

Target ORF information:

RefSeq Version	NM_139462.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster supervillin, transcript variant AF (Svil), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139462.4

ORF Insert Sequence:

ATGTATCTCA TACAGACTGT TCAAACCACT TGGCGGCCAC AATTGCCGCT GCCCAGGGAG 
CTTCAGCTGA CCAATGACCG GAATAGCGCC GACGACCACC ACGATTCAAA GGTGATAACG 
ACCACCACAA CCACAGCGGA CATCCATCAC CACAACCAGC AGGAGGAGAA CGAGGAAGAG 
GATATGGATC GGCTAAGGGG CAATCACAGC ATTGGAGGAT CCTCGCAGCA GCTTTCTGGC 
AAACAGAAAT GGTCCTTCGG GCGACTTTTT CGCAAGAAAA AGGAGGCGGA GAGTGCATCC 
TCCAGCGAAG AAGATCGCAA GGCGGGATTT GTGCCTGCCC AGCGCCAGTC AGCCAGCAAG 
CCGAAGGGAA AACATGGCAA AGGATCGCGA GGTTGCAGCA AATTCGATCA CATTGTGGTG 
AGTCAGCGGC AAGAGCAGGC TCCACCACAT CCACCCCAAC CGCAAATCGA CAATGAGTTC 
TTCTTGCCCT TGGAGGCCAT ACAACCGGAG CCCTACTATC AAAATCAACC GGGCTACTAT 
CAACGATCGA GTAACTCGCT GGACAGGAGA TTACTAATGC AGCCTGGAGT TCAGGGAAAT 
GGTTACCAGC AGCCCAAATC TCGATCGGCG CAGCGAGGTC CCAAGCCACC AGGAGCTCAC 
TCCAGCGAGG AGGAACTGAT CTCGCTGAAC TCCTCCACGT TCTCCAAATA CCGCAGCGAT 
GAGAGTATTC ATTTGGGTTC CGCTGCTGGA CAAAGTCGAC GCAGTCGAGC GGCCCGCAAT 
GAGCGTTACT ACAAGAGATT GTCCCGGGAT GGCGAACCGA GTCATGGCGG AGCACCCGTT 
GTAGCCCAGC AGCGATACAA AACCCAGCCA CTGCCTTTAT CCATTTACCA GCCAACGAAC 
GCGTCCACTG GCTATGTCCA GTGGCAACCG CAGCCCCAGA AGAACCTGCA GAATGCGATG 
CAGAACGATG GAAAGCGAAG CATCAGCTAC GACAGTCAAA TACACCTGCA GAACATGAAT 
GGGAGGATGC AGAGTAAGCC ATTGCCACCG CCACCGCCTC CAAGGGATCC ACTGAGGAGA 
GTGCATGTTG GAGGTTCCGG GAAGATTGGA TCCAGCTCTG GAGACTTGCG TCCCGTGTCC 
TATGCCTTCG ATCACAATAG TCGCTGTGCG TCGGATGATC GCATTTGGCA GCCACCCCAC 
TACCAGTCGG TGCATTCGCT CAACTCCCAG CCCAGTTCGT CAATAGTCAG TGCACCAGTG 
AGTCAACAGC AACCGCACCA CAGGAGATTC ATAACTCGAT CCGAGAGGAA CGCAAATCCT 
GGCAAGCAAT CCCTGCCGAA TGGTATTGAT TTCCATTATG TGGCAGATGC AACGCCACGC 
TCCAGGAAGC CCATACACAT GATGGATGCA GGCAGGATGG ACAATACAGG AGTGCCAGCA 
TCCAGTGGTA TATTGCGAAC CTCTAAGAGT GGGCTGCCCA CACCAAGTCC TGCTCCCCAG 
CAGCCCTTGG CCAAACCGCG GTCGTTCTCC AGCTCGCGAC TTAGTGAGCT GCGAACCTAT 
CCCATGCCCA TGTACTCCGA GGTGCAGAAA CCTAAGAGGA ATGCGCCACC AGTCAATCCC 
TCGCCGAATC AGGATAACAT AGTGTGTGGG AGTCTGCACA TAAAACCCAC ACAAGAGCAT 
GGAAATCAAA ACTATGTGGA GATTCGTCAT CGGGATGTGC ACAAGACCAA CTCAATGCCG 
CACCATTATC AGAATAGGCG ATCTGGCGAG GATTTACCCA ACAAATATGA GGAGTACGTG 
GCAGAAAAGA GGGAGCAGCA TCAACAGGCA CCACCCCCAG TTTATCCGGA GCGCAAAATG 
AGCTTGACAC CAGCAGTGGC GCCACCGCCT CCTGTTTACT TCCCTCGCAA GAAGCCTGCC 
AATTTGGAGG AGGCCATCAA CGAACTGGAG GCCATTTACA AGTCTCTAGG ACTCACTGAA 
CCAGCCGAGC CCAAACAGGA ACCAAAGCTG GATATTTCCT CCAAGATGCG TGTGCCAACT 
CCCAGTGATT TTGAAAAGTT CGCCTTGGCG CATGCTGATG ACTACGAGGA TGAAGATTCG 
CCAACCGGAG AACCAGATCC AATCAGAGAT GATGTGGCCT TCAGGAACCT ACAGTTGGCC 
AATCTGCAAC ATCGGCCATC GGAGAAGCAG CCTCCATTCG GCATCCCAGT GGGACCCATT 
GTGCCAGCTC CACAGTCGGA TTATCTTCAC GTAGAGCCAG TGAGGGTGAA GAAGAAGAGT 
GGCTCTCCTG ACATCATTAA AGATGATCTT GCTGTGCGTT CATTGAGAAA GGATCCTCCT 
GGTCCCAAAT CCAGCTTTGT CTACCCAATG CAAAAGAAGC AACGGGCTAT TCGAACCCAA 
TCGGCCAACA TATACAATCT GATCCAAAGA GACGCTGCCA AGCCATCTGG TGGAGATCTT 
AAGAGCTACA TGGAGCTGAC TCGCAACCTG GAAAGAGCTG GCAGCATGTC CAATCTGCAG 
GGTGAGGATG GCAGTGATAC CAAAGATGTA CCCGCCACAC TGGATCTTCT CCGAAAGTTG 
AAAGCTCAGG ATGAGGAACT GGATTTGGCT AGAAAGCATC ACCCCATACC GTTTAGACAT 
CCCAGTCAGG GAGGAGCTAT TGCCCAACTG CCAGAACGGT TGTCCAAAGA GGAAATACCC 
AAGGCAGCGC CTGTGCCAGC TCCCCGAAAG AGTCTGACTC CTGAACCCAT GCTAGATGAT 
GCTCTCAACA AGATTGCCCA GGATGCGCAG GCCAGCAGCA TTAAGCTGAG TCAGGAACTC 
CATGAACTTC GCAAGGAGGC ATTGATTACA GCTGCGAGAC CAAAGTTGAA TGCCGAACAA 
CAGAGATTAG AGGATGAACT GCAGCAGATA GAAGCTGTCT CCGAGGCAGC CAAACGTTGT 
GGCCAAATGC TGCTCGAAAC TCTGCCAGAT GCGGGACAGG AGGGTCAGGA ACCTCAGGAT 
AAGCCCCTCA GGAAGCTACA TAAGGAGGGC AAACTCATAC GAGCTATCGA TGAGGTGTCC 
GAAGCTGCTA ATGCGGTCTG TGAGAAGATC CTCAAGGACA TTGTGACCAC AGAACCACCG 
GTGGTAGTTC AGGAGGCCCT CCAAGTGAGG CAGACCACAA AGACTGGACC AGGCGGAGAA 
CAGCTGGTGA TGCCGCATCT TATCAAGAAA CTAGATCCCA TACAATCCGA CAAGATCGAG 
CACATTGCCA GACGATGCAT GCGGCAGTTG AGCGAACTGG CCAATAATCC GGACTACGAC 
AACCTGGCCA CCTGTAATAT GAGCAACAGC ACCACCAACG CAACGGGTGG AGCAGCCGCT 
GATGCCACTC TCGTTTGTCC CGTTCCAGAG ACCCAAGCCA AGAGCCAGAC GCTCGAGGAG 
ATTGACAAGA TCATGCAGGA GTGCGAGCGT CAGGCGAAGA GCAGCAGCTC AACCACGACC 
ACCGATGATC AGCGCACCAC TGCCCCCAGT CTGACGAGTG GAAGTGGCAG CGGGAGCTTC 
CCACCCAATG TGAGCACCAC CGCCAGTTCG TTCAGCTCAA GCAGCGACTG TTTGGCCAAG 
TCGTCGAGTC CTTCGCGCGC CAGCCGTCCG CTCTCCTCGA GCATCACCTC TTTCAATCCA 
TATTCCTCCA GCGACTACAT CAAGTCGCAG AGCAGTGATT GCCATGCACC AAGCACCGAT 
CCGATCAAGA CCTTTAGCAC CACCTCGTAC GAGGTGCAGT CCACACCGCA GAGCACCACC 
ATTAGCACCA ATACGATGAG CACGAGCCAG AGCAACACGA TCAGTAGCAC TACTAATCCG 
AGTGTGGAGT CCAAGCCATC CGCCCCGTCG GTATCGCCAC CACCCCTCCA GTTGACGCCA 
GTGGGTGATC GGAGGGGCAG GAACCGGGAG CGGGGAGCTT CCTGCTCCTC CACATCCTCA 
CCTTCACAGT ACAACTCGAG CGAGGAGCTG GCCGCCATTT TTGGCATCGA GGAGCAACCG 
AAACAGCAGC GTCGCAGTCG GCAGACGCAT AATGCCACAG GTAACGGAGA TGATCCCGCC 
ACCACTGAGG CCCCGAGTTC GCCTGATTCC GATTCCGCCA ACGCCAATGA TACTGCCAAT 
GCCAATGCCA ATACTACTAA TCCCGCCCAG CTCGATTCAG CTAATTGCGC TACTAATCCC 
GTTATGAATG TTCAACTTGA TGTTACTACT AGCACCTCCA CCTCAGCCTC AACCACCCAT 
CCAACCTCTA ACGATAATGC TAGCACTTGT AGTAGCCATA GTTATAAGCT AGAGACGATT 
CCGGAGCAGG ATCACGAGGA GGTGGAGTCC ACGCTAAAGT TGTCGCCGAA CTTCAATAGG 
ATTTATGCCA GCAAGATTCG CATAGTAATG GATCGCAATG AAGAGGCAAG TCTCCTGCCG 
GAAGGAGCAG CTAATGTTGC TTTAACTCAA TTCGATTCTT TGAGTTCTCT GGAAGAGACC 
TCGTCCAATC CGGACTCTGG GAATGTTAGT CTCACGGATT ATCCCCTGGC TAACCATGCG 
AATTCCTCCC TGGAACTGCA GACCGCTTAC AATAGTCGAT TTCAAGTGAG GGAGCATTAC 
GTATCCGAGG TTTCCATTAG TGCTTCGGAA TCAACGGATG AGGAGCAGAC GGACACGGAG 
TGCTTAAGAG ATGCTGATCG TTTGAACACA GAAGACTTTC CACCTTTACC CACAGAAGAA 
GAGCTAGAGG AGAGTCTACG GGAAGAAATA GAACTTAACG ATAGTCTTAG TGGGGGCACT 
TTAAATATTG CTAGAGAGAG TAGCGGAGAA TTGGTATCTG TAGTTCAAGA ATTGAACCTG 
AATGAAACTG TTAGTCTGAG TCAAGAGGTT TACAATGTAA CCACTGAGGC GCTGCAACGA 
ATAGCGACAG ATGAAATAGA TGGCTTAAGT TCCGCTATAA TTGAGGTTAA CGAAAGCTTA 
CTTAACGAAT CTGCATTTGA TGAGATGCAC TTTAAACTAG ACGAAACGGA ACTGGACGAA 
TCGGAAATCA TAGACAGAAT GCTTGAGGCT TCATTTGACG AGTTAGACGA ATTAGAACTT 
GAGAACACAG AACTTGACGA AAGTCGACTT GACGACGAAC AAAAGAGCGA ATGCCAATTT 
AACGAAACCT ATAACGAGTT GGACAGCATA GATCTTGATG CCACAGAACT TAACGCCACT 
GGACTTAACG AAATTTCATA TGACCAGCTG GAGGAACTAG AACTTGACGA TAGCGACTCC 
GTAATGCCGT TCACACGGCG CAAGAGCATC GTTTACGAAA CGCCACAACT TAACCTGAGC 
ATTTCCGAAC TAAAAGTGAA TTACGAAACG CAAGTGGAGC CAGTAATTAA ATCAGAGTCA 
CTCAACGAAG CCATTAAATC GATGAACAAC GACGATGAAG GCGACGAGGA GATGCAGGCA 
GGCAGTGAAG AGAAGGTAGA TGAAGAGGAA AACAGTGTAA CTGAGACCCA GACACATCGT 
CGTCGGTGCA AAGGACTTCC GGCGAATCCC GCTAATGATG ATGAACGGCC GTCCACCAGC 
TGCAAGGCTC TTTCCGTTTT TGGCGAGGTG CGAACTGTGG CACCACAGGC GGCGGCGGCA 
GAGGCAGCTG CAGCAGTTGG TGGTGCAACG CGGGGCGGTT GGACTACACT CGAGCACGTA 
TTTGTCGCCT GCACTGTGGG CCTGATTACA CCGAACGATT TGCTCACTTT GTGCCTGATC 
GTAATTGGTG TGATTATCAT TATTGCAATT GCTCTCACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Svil ( NM_001202097.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001189026.1 Drosophila melanogaster supervillin, transcript variant AH (Svil), mRNA.

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CloneID	OFa19994
Clone ID Related Accession (Same CDS sequence)	NM_001202097.2 , NM_001202098.2
Accession Version	NM_001202097.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	supervillin, isoform AH
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001202097.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCGGC TAAGGGGCAA TCACAGCATT GGAGGATCCT CGCAGCAGCT TTCTGGCAAA 
CAGAAATGGT CCTTCGGGCG ACTTTTTCGC AAGAAAAAGG AGGCGGAGAG TGCATCCTCC 
AGCGAAGAAG ATCGCAAGGC GGGATTTGTG CCTGCCCAGC GCCAGTCAGC CAGCAAGCCG 
AAGGGAAAAC ATGGCAAAGG ATCGCGAGGT TGCAGCAAAT TCGATCACAT TGTGGTGAGT 
CAGCGGCAAG AGCAGGCTCC ACCACATCCA CCCCAACCGC AAATCGACAA TGAGTTCTTC 
TTGCCCTTGG AGGCCATACA ACCGGAGCCC TACTATCAAA ATCAACCGGG CTACTATCAA 
CGATCGAGTA ACTCGCTGGA CAGGAGATTA CTAATGCAGC CTGGAGTTCA GGGAAATGGT 
TACCAGCAGC CCAAATCTCG ATCGGCGCAG CGAGGTCCCA AGCCACCAGG AGCTCACTCC 
AGCGAGGAGG AACTGATCTC GCTGAACTCC TCCACGTTCT CCAAATACCG CAGCGATGAG 
AGTATTCATT TGGGTTCCGC TGCTGGACAA AGTCGACGCA GTCGAGCGGC CCGCAATGAG 
CGTTACTACA AGAGATTGTC CCGGGATGGC GAACCGAGTC ATGGCGGAGC ACCCGTTGTA 
GCCCAGCAGC GATACAAAAC CCAGCCACTG CCTTTATCCA TTTACCAGCC AACGAACGCG 
TCCACTGGCT ATGTCCAGTG GCAACCGCAG CCCCAGAAGA ACCTGCAGAA TGCGATGCAG 
AACGATGGAA AGCGAAGCAT CAGCTACGAC AGTCAAATAC ACCTGCAGAA CATGAATGGG 
AGGATGCAGA GTAAGCCATT GCCACCGCCA CCGCCTCCAA GGGATCCACT GAGGAGAGTG 
CATGTTGGAG GTTCCGGGAA GATTGGATCC AGCTCTGGAG ACTTGCGTCC CGTGTCCTAT 
GCCTTCGATC ACAATAGTCG CTGTGCGTCG GATGATCGCA TTTGGCAGCC ACCCCACTAC 
CAGTCGGTGC ATTCGCTCAA CTCCCAGCCC AGTTCGTCAA TAGTCAGTGC ACCAGTGAGT 
CAACAGCAAC CGCACCACAG GAGATTCATA ACTCGATCCG AGAGGAACGC AAATCCTGGC 
AAGCAATCCC TGCCGAATGG TATTGATTTC CATTATGTGG CAGATGCAAC GCCACGCTCC 
AGGAAGCCCA TACACATGAT GGATGCAGGC AGGATGGACA ATACAGGAGT GCCAGCATCC 
AGTGGTATAT TGCGAACCTC TAAGAGTGGG CTGCCCACAC CAAGTCCTGC TCCCCAGCAG 
CCCTTGGCCA AACCGCGGTC GTTCTCCAGC TCGCGACTTA GTGAGCTGCG AACCTATCCC 
ATGCCCATGT ACTCCGAGGT GCAGAAACCT AAGAGGAATG CGCCACCAGT CAATCCCTCG 
CCGAATCAGG ATAACATAGT GTGTGGGAGT CTGCACATAA AACCCACACA AGAGCATGGA 
AATCAAAACT ATGTGGAGAT TCGTCATCGG GATGTGCACA AGACCAACTC AATGCCGCAC 
CATTATCAGA ATAGGCGATC TGGCGAGGAT TTACCCAACA AATATGAGGA GTACGTGGCA 
GAAAAGAGGG AGCAGCATCA ACAGGCACCA CCCCCAGTTT ATCCGGAGCG CAAAATGAGC 
TTGACACCAG CAGTGGCGCC ACCGCCTCCT GTTTACTTCC CTCGCAAGAA GCCTGCCAAT 
TTGGAGGAGG CCATCAACGA ACTGGAGGCC ATTTACAAGT CTCTAGGACT CACTGAACCA 
GCCGAGCCCA AACAGGAACC AAAGCTGGAT ATTTCCTCCA AGATGCGTGT GCCAACTCCC 
AGTGATTTTG AAAAGTTCGC CTTGGCGCAT GCTGATGACT ACGAGGATGA AGATTCGCCA 
ACCGGAGAAC CAGATCCAAT CAGAGATGAT GTGGCCTTCA GGAACCTACA GTTGGCCAAT 
CTGCAACATC GGCCATCGGA GAAGCAGCCT CCATTCGGCA TCCCAGTGGG ACCCATTGTG 
CCAGCTCCAC AGTCGGATTA TCTTCACGTA GAGCCAGTGA GGGTGAAGAA GAAGAGTGGC 
TCTCCTGACA TCATTAAAGA TGATCTTGCT GTGCGTTCAT TGAGAAAGGA TCCTCCTGGT 
CCCAAATCCA GCTTTGTCTA CCCAATGCAA AAGAAGCAAC GGGCTATTCG AACCCAATCG 
GCCAACATAT ACAATCTGAT CCAAAGAGAC GCTGCCAAGC CATCTGGTGG AGATCTTAAG 
AGCTACATGG AGCTGACTCG CAACCTGGAA AGAGCTGGCA GCATGTCCAA TCTGCAGGGT 
GAGGATGGCA GTGATACCAA AGATGTACCC GCCACACTGG ATCTTCTCCG AAAGTTGAAA 
GCTCAGGATG AGGAACTGGA TTTGGCTAGA AAGCATCACC CCATACCGTT TAGACATCCC 
AGTCAGGGAG GAGCTATTGC CCAACTGCCA GAACGGTTGT CCAAAGAGGA AATACCCAAG 
GCAGCGCCTG TGCCAGCTCC CCGAAAGAGT CTGACTCCTG AACCCATGCT AGATGATGCT 
CTCAACAAGA TTGCCCAGGA TGCGCAGGCC AGCAGCATTA AGCTGAGTCA GGAACTCCAT 
GAACTTCGCA AGGAGGCATT GATTACAGCT GCGAGACCAA AGTTGAATGC CGAACAACAG 
AGATTAGAGG ATGAACTGCA GCAGATAGAA GCTGTCTCCG AGGCAGCCAA ACGTTGTGGC 
CAAATGCTGC TCGAAACTCT GCCAGATGCG GGACAGGAGG GTCAGGAACC TCAGGATAAG 
CCCCTCAGGA AGCTACATAA GGAGGGCAAA CTCATACGAG CTATCGATGA GGTGTCCGAA 
GCTGCTAATG CGGTCTGTGA GAAGATCCTC AAGGACATTG TGACCACAGA ACCACCGGTG 
GTAGTTCAGG AGGCCCTCCA AGTGAGGCAG ACCACAAAGA CTGGACCAGG CGGAGAACAG 
CTGGTGATGC CGCATCTTAT CAAGAAACTA GATCCCATAC AATCCGACAA GATCGAGCAC 
ATTGCCAGAC GATGCATGCG GCAGTTGAGC GAACTGGCCA ATAATCCGGA CTACGACAAC 
CTGGCCACCT GTAATATGAG CAACAGCACC ACCAACGCAA CGGGTGGAGC AGCCGCTGAT 
GCCACTCTCG TTTGTCCCGT TCCAGAGACC CAAGCCAAGA GCCAGACGCT CGAGGAGATT 
GACAAGATCA TGCAGGAGTG CGAGCGTCAG GCGAAGAGCA GCAGCTCAAC CACGACCACC 
GATGATCAGC GCACCACTGC CCCCAGTCTG ACGAGTGGAA GTGGCAGCGG GAGCTTCCCA 
CCCAATGTGA GCACCACCGC CAGTTCGTTC AGCTCAAGCA GCGACTGTTT GGCCAAGTCG 
TCGAGTCCTT CGCGCGCCAG CCGTCCGCTC TCCTCGAGCA TCACCTCTTT CAATCCATAT 
TCCTCCAGCG ACTACATCAA GTCGCAGAGC AGTGATTGCC ATGCACCAAG CACCGATCCG 
ATCAAGACCT TTAGCACCAC CTCGTACGAG GTGCAGTCCA CACCGCAGAG CACCACCATT 
AGCACCAATA CGATGAGCAC GAGCCAGAGC AACACGATCA GTAGCACTAC TAATCCGAGT 
GTGGAGTCCA AGCCATCCGC CCCGTCGGTA TCGCCACCAC CCCTCCAGTT GACGCCAGTG 
GGTGATCGGA GGGGCAGGAA CCGGGAGCGG GGAGCTTCCT GCTCCTCCAC ATCCTCACCT 
TCACAGTACA ACTCGAGCGA GGAGCTGGCC GCCATTTTTG GCATCGAGGA GCAACCGAAA 
CAGCAGCGTC GCAGTCGGCA GACGCATAAT GCCACAGGTA ACGGAGATGA TCCCGCCACC 
ACTGAGGCCC CGAGTTCGCC TGATTCCGAT TCCGCCAACG CCAATGATAC TGCCAATGCC 
AATGCCAATA CTACTAATCC CGCCCAGCTC GATTCAGCTA ATTGCGCTAC TAATCCCGTT 
ATGAATGTTC AACTTGATGT TACTACTAGC ACCTCCACCT CAGCCTCAAC CACCCATCCA 
ACCTCTAACG ATAATGCTAG CACTTGTAGT AGCCATAGTT ATAAGCTAGA GACGATTCCG 
GAGCAGGATC ACGAGGAGGT GGAGTCCACG CTAAAGTTGT CGCCGAACTT CAATAGGATT 
TATGCCAGCA AGATTCGCAT AGTAATGGAT CGCAATGAAG AGGCAAGTCT CCTGCCGGAA 
GGAGCAGCTA ATGTTGCTTT AACTCAATTC GATTCTTTGA GTTCTCTGGA AGAGACCTCG 
TCCAATCCGG ACTCTGGGAA TGTTAGTCTC ACGGATTATC CCCTGGCTAA CCATGCGAAT 
TCCTCCCTGG AACTGCAGAC CGCTTACAAT AGTCGATTTC AAGTGAGGGA GCATTACGTA 
TCCGAGGTTT CCATTAGTGC TTCGGAATCA ACGGATGAGG AGCAGACGGA CACGGAGTGC 
TTAAGAGATG CTGATCGTTT GAACACAGAA GACTTTCCAC CTTTACCCAC AGAAGAAGAG 
CTAGAGGAGA GTCTACGGGA AGAAATAGAA CTTAACGATA GTCTTAGTGG GGGCACTTTA 
AATATTGCTA GAGAGAGTAG CGGAGAATTG GTATCTGTAG TTCAAGAATT GAACCTGAAT 
GAAACTGTTA GTCTGAGTCA AGAGGTTTAC AATGTAACCA CTGAGGCGCT GCAACGAATA 
GCGACAGATG AAATAGATGG CTTAAGTTCC GCTATAATTG AGGTTAACGA AAGCTTACTT 
AACGAATCTG CATTTGATGA GATGCACTTT AAACTAGACG AAACGGAACT GGACGAATCG 
GAAATCATAG ACAGAATGCT TGAGGCTTCA TTTGACGAGT TAGACGAATT AGAACTTGAG 
AACACAGAAC TTGACGAAAG TCGACTTGAC GACGAACAAA AGAGCGAATG CCAATTTAAC 
GAAACCTATA ACGAGTTGGA CAGCATAGAT CTTGATGCCA CAGAACTTAA CGCCACTGGA 
CTTAACGAAA TTTCATATGA CCAGCTGGAG GAACTAGAAC TTGACGATAG CGACTCCGTA 
ATGCCGTTCA CACGGCGCAA GAGCATCGTT TACGAAACGC CACAACTTAA CCTGAGCATT 
TCCGAACTAA AAGTGAATTA CGAAACGCAA GTGGAGCCAG TAATTAAATC AGAGTCACTC 
AACGAAGCCA TTAAATCGAT GAACAACGAC GATGAAGGCG ACGAGGAGAT GCAGGCAGGC 
AGTGAAGAGA AGGTAGATGA AGAGGAAAAC AGTGTAACTG AGACCCAGAC ACATCGTCGT 
CGGTGCAAAG GACTTCCGGC GAATCCCGCT AATGATGATG AACGGCCGTC CACCAGCTGC 
AAGGCTCTTT CCGTTTTTGG CGAGGTGCGA ACTGTGGCAC CACAGGCGGC GGCGGCAGAG 
GCAGCTGCAG CAGTTGGTGG TGCAACGCGG GGCGGTTGGA CTACACTCGA GCACGTATTT 
GTCGCCTGCA CTGTGGGCCT GATTACACCG AACGATTTGC TCACTTTGTG CCTGATCGTA 
ATTGGTGTGA TTATCATTAT TGCAATTGCT CTCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189026.1
CDS	786..6404
Translation	MDRLRGNHSIGGSSQQLSGKQKWSFGRLFRKKKEAESASSSEEDRKAGFVPAQRQSASKPKGKHGKGSRGCSKFDHIVVSQRQEQAPPHPPQPQIDNEFFLPLEAIQPEPYYQNQPGYYQRSSNSLDRRLLMQPGVQGNGYQQPKSRSAQRGPKPPGAHSSEEELISLNSSTFSKYRSDESIHLGSAAGQSRRSRAARNERYYKRLSRDGEPSHGGAPVVAQQRYKTQPLPLSIYQPTNASTGYVQWQPQPQKNLQNAMQNDGKRSISYDSQIHLQNMNGRMQSKPLPPPPPPRDPLRRVHVGGSGKIGSSSGDLRPVSYAFDHNSRCASDDRIWQPPHYQSVHSLNSQPSSSIVSAPVSQQQPHHRRFITRSERNANPGKQSLPNGIDFHYVADATPRSRKPIHMMDAGRMDNTGVPASSGILRTSKSGLPTPSPAPQQPLAKPRSFSSSRLSELRTYPMPMYSEVQKPKRNAPPVNPSPNQDNIVCGSLHIKPTQEHGNQNYVEIRHRDVHKTNSMPHHYQNRRSGEDLPNKYEEYVAEKREQHQQAPPPVYPERKMSLTPAVAPPPPVYFPRKKPANLEEAINELEAIYKSLGLTEPAEPKQEPKLDISSKMRVPTPSDFEKFALAHADDYEDEDSPTGEPDPIRDDVAFRNLQLANLQHRPSEKQPPFGIPVGPIVPAPQSDYLHVEPVRVKKKSGSPDIIKDDLAVRSLRKDPPGPKSSFVYPMQKKQRAIRTQSANIYNLIQRDAAKPSGGDLKSYMELTRNLERAGSMSNLQGEDGSDTKDVPATLDLLRKLKAQDEELDLARKHHPIPFRHPSQGGAIAQLPERLSKEEIPKAAPVPAPRKSLTPEPMLDDALNKIAQDAQASSIKLSQELHELRKEALITAARPKLNAEQQRLEDELQQIEAVSEAAKRCGQMLLETLPDAGQEGQEPQDKPLRKLHKEGKLIRAIDEVSEAANAVCEKILKDIVTTEPPVVVQEALQVRQTTKTGPGGEQLVMPHLIKKLDPIQSDKIEHIARRCMRQLSELANNPDYDNLATCNMSNSTTNATGGAAADATLVCPVPETQAKSQTLEEIDKIMQECERQAKSSSSTTTTDDQRTTAPSLTSGSGSGSFPPNVSTTASSFSSSSDCLAKSSSPSRASRPLSSSITSFNPYSSSDYIKSQSSDCHAPSTDPIKTFSTTSYEVQSTPQSTTISTNTMSTSQSNTISSTTNPSVESKPSAPSVSPPPLQLTPVGDRRGRNRERGASCSSTSSPSQYNSSEELAAIFGIEEQPKQQRRSRQTHNATGNGDDPATTEAPSSPDSDSANANDTANANANTTNPAQLDSANCATNPVMNVQLDVTTSTSTSASTTHPTSNDNASTCSSHSYKLETIPEQDHEEVESTLKLSPNFNRIYASKIRIVMDRNEEASLLPEGAANVALTQFDSLSSLEETSSNPDSGNVSLTDYPLANHANSSLELQTAYNSRFQVREHYVSEVSISASESTDEEQTDTECLRDADRLNTEDFPPLPTEEELEESLREEIELNDSLSGGTLNIARESSGELVSVVQELNLNETVSLSQEVYNVTTEALQRIATDEIDGLSSAIIEVNESLLNESAFDEMHFKLDETELDESEIIDRMLEASFDELDELELENTELDESRLDDEQKSECQFNETYNELDSIDLDATELNATGLNEISYDQLEELELDDSDSVMPFTRRKSIVYETPQLNLSISELKVNYETQVEPVIKSESLNEAIKSMNNDDEGDEEMQAGSEEKVDEEENSVTETQTHRRRCKGLPANPANDDERPSTSCKALSVFGEVRTVAPQAAAAEAAAAVGGATRGGWTTLEHVFVACTVGLITPNDLLTLCLIVIGVIIIIAIALT

Target ORF information:

RefSeq Version	NM_001202097.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster supervillin, transcript variant AH (Svil), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202097.2

ORF Insert Sequence:

ATGGATCGGC TAAGGGGCAA TCACAGCATT GGAGGATCCT CGCAGCAGCT TTCTGGCAAA 
CAGAAATGGT CCTTCGGGCG ACTTTTTCGC AAGAAAAAGG AGGCGGAGAG TGCATCCTCC 
AGCGAAGAAG ATCGCAAGGC GGGATTTGTG CCTGCCCAGC GCCAGTCAGC CAGCAAGCCG 
AAGGGAAAAC ATGGCAAAGG ATCGCGAGGT TGCAGCAAAT TCGATCACAT TGTGGTGAGT 
CAGCGGCAAG AGCAGGCTCC ACCACATCCA CCCCAACCGC AAATCGACAA TGAGTTCTTC 
TTGCCCTTGG AGGCCATACA ACCGGAGCCC TACTATCAAA ATCAACCGGG CTACTATCAA 
CGATCGAGTA ACTCGCTGGA CAGGAGATTA CTAATGCAGC CTGGAGTTCA GGGAAATGGT 
TACCAGCAGC CCAAATCTCG ATCGGCGCAG CGAGGTCCCA AGCCACCAGG AGCTCACTCC 
AGCGAGGAGG AACTGATCTC GCTGAACTCC TCCACGTTCT CCAAATACCG CAGCGATGAG 
AGTATTCATT TGGGTTCCGC TGCTGGACAA AGTCGACGCA GTCGAGCGGC CCGCAATGAG 
CGTTACTACA AGAGATTGTC CCGGGATGGC GAACCGAGTC ATGGCGGAGC ACCCGTTGTA 
GCCCAGCAGC GATACAAAAC CCAGCCACTG CCTTTATCCA TTTACCAGCC AACGAACGCG 
TCCACTGGCT ATGTCCAGTG GCAACCGCAG CCCCAGAAGA ACCTGCAGAA TGCGATGCAG 
AACGATGGAA AGCGAAGCAT CAGCTACGAC AGTCAAATAC ACCTGCAGAA CATGAATGGG 
AGGATGCAGA GTAAGCCATT GCCACCGCCA CCGCCTCCAA GGGATCCACT GAGGAGAGTG 
CATGTTGGAG GTTCCGGGAA GATTGGATCC AGCTCTGGAG ACTTGCGTCC CGTGTCCTAT 
GCCTTCGATC ACAATAGTCG CTGTGCGTCG GATGATCGCA TTTGGCAGCC ACCCCACTAC 
CAGTCGGTGC ATTCGCTCAA CTCCCAGCCC AGTTCGTCAA TAGTCAGTGC ACCAGTGAGT 
CAACAGCAAC CGCACCACAG GAGATTCATA ACTCGATCCG AGAGGAACGC AAATCCTGGC 
AAGCAATCCC TGCCGAATGG TATTGATTTC CATTATGTGG CAGATGCAAC GCCACGCTCC 
AGGAAGCCCA TACACATGAT GGATGCAGGC AGGATGGACA ATACAGGAGT GCCAGCATCC 
AGTGGTATAT TGCGAACCTC TAAGAGTGGG CTGCCCACAC CAAGTCCTGC TCCCCAGCAG 
CCCTTGGCCA AACCGCGGTC GTTCTCCAGC TCGCGACTTA GTGAGCTGCG AACCTATCCC 
ATGCCCATGT ACTCCGAGGT GCAGAAACCT AAGAGGAATG CGCCACCAGT CAATCCCTCG 
CCGAATCAGG ATAACATAGT GTGTGGGAGT CTGCACATAA AACCCACACA AGAGCATGGA 
AATCAAAACT ATGTGGAGAT TCGTCATCGG GATGTGCACA AGACCAACTC AATGCCGCAC 
CATTATCAGA ATAGGCGATC TGGCGAGGAT TTACCCAACA AATATGAGGA GTACGTGGCA 
GAAAAGAGGG AGCAGCATCA ACAGGCACCA CCCCCAGTTT ATCCGGAGCG CAAAATGAGC 
TTGACACCAG CAGTGGCGCC ACCGCCTCCT GTTTACTTCC CTCGCAAGAA GCCTGCCAAT 
TTGGAGGAGG CCATCAACGA ACTGGAGGCC ATTTACAAGT CTCTAGGACT CACTGAACCA 
GCCGAGCCCA AACAGGAACC AAAGCTGGAT ATTTCCTCCA AGATGCGTGT GCCAACTCCC 
AGTGATTTTG AAAAGTTCGC CTTGGCGCAT GCTGATGACT ACGAGGATGA AGATTCGCCA 
ACCGGAGAAC CAGATCCAAT CAGAGATGAT GTGGCCTTCA GGAACCTACA GTTGGCCAAT 
CTGCAACATC GGCCATCGGA GAAGCAGCCT CCATTCGGCA TCCCAGTGGG ACCCATTGTG 
CCAGCTCCAC AGTCGGATTA TCTTCACGTA GAGCCAGTGA GGGTGAAGAA GAAGAGTGGC 
TCTCCTGACA TCATTAAAGA TGATCTTGCT GTGCGTTCAT TGAGAAAGGA TCCTCCTGGT 
CCCAAATCCA GCTTTGTCTA CCCAATGCAA AAGAAGCAAC GGGCTATTCG AACCCAATCG 
GCCAACATAT ACAATCTGAT CCAAAGAGAC GCTGCCAAGC CATCTGGTGG AGATCTTAAG 
AGCTACATGG AGCTGACTCG CAACCTGGAA AGAGCTGGCA GCATGTCCAA TCTGCAGGGT 
GAGGATGGCA GTGATACCAA AGATGTACCC GCCACACTGG ATCTTCTCCG AAAGTTGAAA 
GCTCAGGATG AGGAACTGGA TTTGGCTAGA AAGCATCACC CCATACCGTT TAGACATCCC 
AGTCAGGGAG GAGCTATTGC CCAACTGCCA GAACGGTTGT CCAAAGAGGA AATACCCAAG 
GCAGCGCCTG TGCCAGCTCC CCGAAAGAGT CTGACTCCTG AACCCATGCT AGATGATGCT 
CTCAACAAGA TTGCCCAGGA TGCGCAGGCC AGCAGCATTA AGCTGAGTCA GGAACTCCAT 
GAACTTCGCA AGGAGGCATT GATTACAGCT GCGAGACCAA AGTTGAATGC CGAACAACAG 
AGATTAGAGG ATGAACTGCA GCAGATAGAA GCTGTCTCCG AGGCAGCCAA ACGTTGTGGC 
CAAATGCTGC TCGAAACTCT GCCAGATGCG GGACAGGAGG GTCAGGAACC TCAGGATAAG 
CCCCTCAGGA AGCTACATAA GGAGGGCAAA CTCATACGAG CTATCGATGA GGTGTCCGAA 
GCTGCTAATG CGGTCTGTGA GAAGATCCTC AAGGACATTG TGACCACAGA ACCACCGGTG 
GTAGTTCAGG AGGCCCTCCA AGTGAGGCAG ACCACAAAGA CTGGACCAGG CGGAGAACAG 
CTGGTGATGC CGCATCTTAT CAAGAAACTA GATCCCATAC AATCCGACAA GATCGAGCAC 
ATTGCCAGAC GATGCATGCG GCAGTTGAGC GAACTGGCCA ATAATCCGGA CTACGACAAC 
CTGGCCACCT GTAATATGAG CAACAGCACC ACCAACGCAA CGGGTGGAGC AGCCGCTGAT 
GCCACTCTCG TTTGTCCCGT TCCAGAGACC CAAGCCAAGA GCCAGACGCT CGAGGAGATT 
GACAAGATCA TGCAGGAGTG CGAGCGTCAG GCGAAGAGCA GCAGCTCAAC CACGACCACC 
GATGATCAGC GCACCACTGC CCCCAGTCTG ACGAGTGGAA GTGGCAGCGG GAGCTTCCCA 
CCCAATGTGA GCACCACCGC CAGTTCGTTC AGCTCAAGCA GCGACTGTTT GGCCAAGTCG 
TCGAGTCCTT CGCGCGCCAG CCGTCCGCTC TCCTCGAGCA TCACCTCTTT CAATCCATAT 
TCCTCCAGCG ACTACATCAA GTCGCAGAGC AGTGATTGCC ATGCACCAAG CACCGATCCG 
ATCAAGACCT TTAGCACCAC CTCGTACGAG GTGCAGTCCA CACCGCAGAG CACCACCATT 
AGCACCAATA CGATGAGCAC GAGCCAGAGC AACACGATCA GTAGCACTAC TAATCCGAGT 
GTGGAGTCCA AGCCATCCGC CCCGTCGGTA TCGCCACCAC CCCTCCAGTT GACGCCAGTG 
GGTGATCGGA GGGGCAGGAA CCGGGAGCGG GGAGCTTCCT GCTCCTCCAC ATCCTCACCT 
TCACAGTACA ACTCGAGCGA GGAGCTGGCC GCCATTTTTG GCATCGAGGA GCAACCGAAA 
CAGCAGCGTC GCAGTCGGCA GACGCATAAT GCCACAGGTA ACGGAGATGA TCCCGCCACC 
ACTGAGGCCC CGAGTTCGCC TGATTCCGAT TCCGCCAACG CCAATGATAC TGCCAATGCC 
AATGCCAATA CTACTAATCC CGCCCAGCTC GATTCAGCTA ATTGCGCTAC TAATCCCGTT 
ATGAATGTTC AACTTGATGT TACTACTAGC ACCTCCACCT CAGCCTCAAC CACCCATCCA 
ACCTCTAACG ATAATGCTAG CACTTGTAGT AGCCATAGTT ATAAGCTAGA GACGATTCCG 
GAGCAGGATC ACGAGGAGGT GGAGTCCACG CTAAAGTTGT CGCCGAACTT CAATAGGATT 
TATGCCAGCA AGATTCGCAT AGTAATGGAT CGCAATGAAG AGGCAAGTCT CCTGCCGGAA 
GGAGCAGCTA ATGTTGCTTT AACTCAATTC GATTCTTTGA GTTCTCTGGA AGAGACCTCG 
TCCAATCCGG ACTCTGGGAA TGTTAGTCTC ACGGATTATC CCCTGGCTAA CCATGCGAAT 
TCCTCCCTGG AACTGCAGAC CGCTTACAAT AGTCGATTTC AAGTGAGGGA GCATTACGTA 
TCCGAGGTTT CCATTAGTGC TTCGGAATCA ACGGATGAGG AGCAGACGGA CACGGAGTGC 
TTAAGAGATG CTGATCGTTT GAACACAGAA GACTTTCCAC CTTTACCCAC AGAAGAAGAG 
CTAGAGGAGA GTCTACGGGA AGAAATAGAA CTTAACGATA GTCTTAGTGG GGGCACTTTA 
AATATTGCTA GAGAGAGTAG CGGAGAATTG GTATCTGTAG TTCAAGAATT GAACCTGAAT 
GAAACTGTTA GTCTGAGTCA AGAGGTTTAC AATGTAACCA CTGAGGCGCT GCAACGAATA 
GCGACAGATG AAATAGATGG CTTAAGTTCC GCTATAATTG AGGTTAACGA AAGCTTACTT 
AACGAATCTG CATTTGATGA GATGCACTTT AAACTAGACG AAACGGAACT GGACGAATCG 
GAAATCATAG ACAGAATGCT TGAGGCTTCA TTTGACGAGT TAGACGAATT AGAACTTGAG 
AACACAGAAC TTGACGAAAG TCGACTTGAC GACGAACAAA AGAGCGAATG CCAATTTAAC 
GAAACCTATA ACGAGTTGGA CAGCATAGAT CTTGATGCCA CAGAACTTAA CGCCACTGGA 
CTTAACGAAA TTTCATATGA CCAGCTGGAG GAACTAGAAC TTGACGATAG CGACTCCGTA 
ATGCCGTTCA CACGGCGCAA GAGCATCGTT TACGAAACGC CACAACTTAA CCTGAGCATT 
TCCGAACTAA AAGTGAATTA CGAAACGCAA GTGGAGCCAG TAATTAAATC AGAGTCACTC 
AACGAAGCCA TTAAATCGAT GAACAACGAC GATGAAGGCG ACGAGGAGAT GCAGGCAGGC 
AGTGAAGAGA AGGTAGATGA AGAGGAAAAC AGTGTAACTG AGACCCAGAC ACATCGTCGT 
CGGTGCAAAG GACTTCCGGC GAATCCCGCT AATGATGATG AACGGCCGTC CACCAGCTGC 
AAGGCTCTTT CCGTTTTTGG CGAGGTGCGA ACTGTGGCAC CACAGGCGGC GGCGGCAGAG 
GCAGCTGCAG CAGTTGGTGG TGCAACGCGG GGCGGTTGGA CTACACTCGA GCACGTATTT 
GTCGCCTGCA CTGTGGGCCT GATTACACCG AACGATTTGC TCACTTTGTG CCTGATCGTA 
ATTGGTGTGA TTATCATTAT TGCAATTGCT CTCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Svil ( NM_001202098.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001189027.1 Drosophila melanogaster supervillin, transcript variant AG (Svil), mRNA.

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CloneID	OFa19994
Clone ID Related Accession (Same CDS sequence)	NM_001202097.2 , NM_001202098.2
Accession Version	NM_001202098.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	supervillin, isoform AG
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001202098.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCGGC TAAGGGGCAA TCACAGCATT GGAGGATCCT CGCAGCAGCT TTCTGGCAAA 
CAGAAATGGT CCTTCGGGCG ACTTTTTCGC AAGAAAAAGG AGGCGGAGAG TGCATCCTCC 
AGCGAAGAAG ATCGCAAGGC GGGATTTGTG CCTGCCCAGC GCCAGTCAGC CAGCAAGCCG 
AAGGGAAAAC ATGGCAAAGG ATCGCGAGGT TGCAGCAAAT TCGATCACAT TGTGGTGAGT 
CAGCGGCAAG AGCAGGCTCC ACCACATCCA CCCCAACCGC AAATCGACAA TGAGTTCTTC 
TTGCCCTTGG AGGCCATACA ACCGGAGCCC TACTATCAAA ATCAACCGGG CTACTATCAA 
CGATCGAGTA ACTCGCTGGA CAGGAGATTA CTAATGCAGC CTGGAGTTCA GGGAAATGGT 
TACCAGCAGC CCAAATCTCG ATCGGCGCAG CGAGGTCCCA AGCCACCAGG AGCTCACTCC 
AGCGAGGAGG AACTGATCTC GCTGAACTCC TCCACGTTCT CCAAATACCG CAGCGATGAG 
AGTATTCATT TGGGTTCCGC TGCTGGACAA AGTCGACGCA GTCGAGCGGC CCGCAATGAG 
CGTTACTACA AGAGATTGTC CCGGGATGGC GAACCGAGTC ATGGCGGAGC ACCCGTTGTA 
GCCCAGCAGC GATACAAAAC CCAGCCACTG CCTTTATCCA TTTACCAGCC AACGAACGCG 
TCCACTGGCT ATGTCCAGTG GCAACCGCAG CCCCAGAAGA ACCTGCAGAA TGCGATGCAG 
AACGATGGAA AGCGAAGCAT CAGCTACGAC AGTCAAATAC ACCTGCAGAA CATGAATGGG 
AGGATGCAGA GTAAGCCATT GCCACCGCCA CCGCCTCCAA GGGATCCACT GAGGAGAGTG 
CATGTTGGAG GTTCCGGGAA GATTGGATCC AGCTCTGGAG ACTTGCGTCC CGTGTCCTAT 
GCCTTCGATC ACAATAGTCG CTGTGCGTCG GATGATCGCA TTTGGCAGCC ACCCCACTAC 
CAGTCGGTGC ATTCGCTCAA CTCCCAGCCC AGTTCGTCAA TAGTCAGTGC ACCAGTGAGT 
CAACAGCAAC CGCACCACAG GAGATTCATA ACTCGATCCG AGAGGAACGC AAATCCTGGC 
AAGCAATCCC TGCCGAATGG TATTGATTTC CATTATGTGG CAGATGCAAC GCCACGCTCC 
AGGAAGCCCA TACACATGAT GGATGCAGGC AGGATGGACA ATACAGGAGT GCCAGCATCC 
AGTGGTATAT TGCGAACCTC TAAGAGTGGG CTGCCCACAC CAAGTCCTGC TCCCCAGCAG 
CCCTTGGCCA AACCGCGGTC GTTCTCCAGC TCGCGACTTA GTGAGCTGCG AACCTATCCC 
ATGCCCATGT ACTCCGAGGT GCAGAAACCT AAGAGGAATG CGCCACCAGT CAATCCCTCG 
CCGAATCAGG ATAACATAGT GTGTGGGAGT CTGCACATAA AACCCACACA AGAGCATGGA 
AATCAAAACT ATGTGGAGAT TCGTCATCGG GATGTGCACA AGACCAACTC AATGCCGCAC 
CATTATCAGA ATAGGCGATC TGGCGAGGAT TTACCCAACA AATATGAGGA GTACGTGGCA 
GAAAAGAGGG AGCAGCATCA ACAGGCACCA CCCCCAGTTT ATCCGGAGCG CAAAATGAGC 
TTGACACCAG CAGTGGCGCC ACCGCCTCCT GTTTACTTCC CTCGCAAGAA GCCTGCCAAT 
TTGGAGGAGG CCATCAACGA ACTGGAGGCC ATTTACAAGT CTCTAGGACT CACTGAACCA 
GCCGAGCCCA AACAGGAACC AAAGCTGGAT ATTTCCTCCA AGATGCGTGT GCCAACTCCC 
AGTGATTTTG AAAAGTTCGC CTTGGCGCAT GCTGATGACT ACGAGGATGA AGATTCGCCA 
ACCGGAGAAC CAGATCCAAT CAGAGATGAT GTGGCCTTCA GGAACCTACA GTTGGCCAAT 
CTGCAACATC GGCCATCGGA GAAGCAGCCT CCATTCGGCA TCCCAGTGGG ACCCATTGTG 
CCAGCTCCAC AGTCGGATTA TCTTCACGTA GAGCCAGTGA GGGTGAAGAA GAAGAGTGGC 
TCTCCTGACA TCATTAAAGA TGATCTTGCT GTGCGTTCAT TGAGAAAGGA TCCTCCTGGT 
CCCAAATCCA GCTTTGTCTA CCCAATGCAA AAGAAGCAAC GGGCTATTCG AACCCAATCG 
GCCAACATAT ACAATCTGAT CCAAAGAGAC GCTGCCAAGC CATCTGGTGG AGATCTTAAG 
AGCTACATGG AGCTGACTCG CAACCTGGAA AGAGCTGGCA GCATGTCCAA TCTGCAGGGT 
GAGGATGGCA GTGATACCAA AGATGTACCC GCCACACTGG ATCTTCTCCG AAAGTTGAAA 
GCTCAGGATG AGGAACTGGA TTTGGCTAGA AAGCATCACC CCATACCGTT TAGACATCCC 
AGTCAGGGAG GAGCTATTGC CCAACTGCCA GAACGGTTGT CCAAAGAGGA AATACCCAAG 
GCAGCGCCTG TGCCAGCTCC CCGAAAGAGT CTGACTCCTG AACCCATGCT AGATGATGCT 
CTCAACAAGA TTGCCCAGGA TGCGCAGGCC AGCAGCATTA AGCTGAGTCA GGAACTCCAT 
GAACTTCGCA AGGAGGCATT GATTACAGCT GCGAGACCAA AGTTGAATGC CGAACAACAG 
AGATTAGAGG ATGAACTGCA GCAGATAGAA GCTGTCTCCG AGGCAGCCAA ACGTTGTGGC 
CAAATGCTGC TCGAAACTCT GCCAGATGCG GGACAGGAGG GTCAGGAACC TCAGGATAAG 
CCCCTCAGGA AGCTACATAA GGAGGGCAAA CTCATACGAG CTATCGATGA GGTGTCCGAA 
GCTGCTAATG CGGTCTGTGA GAAGATCCTC AAGGACATTG TGACCACAGA ACCACCGGTG 
GTAGTTCAGG AGGCCCTCCA AGTGAGGCAG ACCACAAAGA CTGGACCAGG CGGAGAACAG 
CTGGTGATGC CGCATCTTAT CAAGAAACTA GATCCCATAC AATCCGACAA GATCGAGCAC 
ATTGCCAGAC GATGCATGCG GCAGTTGAGC GAACTGGCCA ATAATCCGGA CTACGACAAC 
CTGGCCACCT GTAATATGAG CAACAGCACC ACCAACGCAA CGGGTGGAGC AGCCGCTGAT 
GCCACTCTCG TTTGTCCCGT TCCAGAGACC CAAGCCAAGA GCCAGACGCT CGAGGAGATT 
GACAAGATCA TGCAGGAGTG CGAGCGTCAG GCGAAGAGCA GCAGCTCAAC CACGACCACC 
GATGATCAGC GCACCACTGC CCCCAGTCTG ACGAGTGGAA GTGGCAGCGG GAGCTTCCCA 
CCCAATGTGA GCACCACCGC CAGTTCGTTC AGCTCAAGCA GCGACTGTTT GGCCAAGTCG 
TCGAGTCCTT CGCGCGCCAG CCGTCCGCTC TCCTCGAGCA TCACCTCTTT CAATCCATAT 
TCCTCCAGCG ACTACATCAA GTCGCAGAGC AGTGATTGCC ATGCACCAAG CACCGATCCG 
ATCAAGACCT TTAGCACCAC CTCGTACGAG GTGCAGTCCA CACCGCAGAG CACCACCATT 
AGCACCAATA CGATGAGCAC GAGCCAGAGC AACACGATCA GTAGCACTAC TAATCCGAGT 
GTGGAGTCCA AGCCATCCGC CCCGTCGGTA TCGCCACCAC CCCTCCAGTT GACGCCAGTG 
GGTGATCGGA GGGGCAGGAA CCGGGAGCGG GGAGCTTCCT GCTCCTCCAC ATCCTCACCT 
TCACAGTACA ACTCGAGCGA GGAGCTGGCC GCCATTTTTG GCATCGAGGA GCAACCGAAA 
CAGCAGCGTC GCAGTCGGCA GACGCATAAT GCCACAGGTA ACGGAGATGA TCCCGCCACC 
ACTGAGGCCC CGAGTTCGCC TGATTCCGAT TCCGCCAACG CCAATGATAC TGCCAATGCC 
AATGCCAATA CTACTAATCC CGCCCAGCTC GATTCAGCTA ATTGCGCTAC TAATCCCGTT 
ATGAATGTTC AACTTGATGT TACTACTAGC ACCTCCACCT CAGCCTCAAC CACCCATCCA 
ACCTCTAACG ATAATGCTAG CACTTGTAGT AGCCATAGTT ATAAGCTAGA GACGATTCCG 
GAGCAGGATC ACGAGGAGGT GGAGTCCACG CTAAAGTTGT CGCCGAACTT CAATAGGATT 
TATGCCAGCA AGATTCGCAT AGTAATGGAT CGCAATGAAG AGGCAAGTCT CCTGCCGGAA 
GGAGCAGCTA ATGTTGCTTT AACTCAATTC GATTCTTTGA GTTCTCTGGA AGAGACCTCG 
TCCAATCCGG ACTCTGGGAA TGTTAGTCTC ACGGATTATC CCCTGGCTAA CCATGCGAAT 
TCCTCCCTGG AACTGCAGAC CGCTTACAAT AGTCGATTTC AAGTGAGGGA GCATTACGTA 
TCCGAGGTTT CCATTAGTGC TTCGGAATCA ACGGATGAGG AGCAGACGGA CACGGAGTGC 
TTAAGAGATG CTGATCGTTT GAACACAGAA GACTTTCCAC CTTTACCCAC AGAAGAAGAG 
CTAGAGGAGA GTCTACGGGA AGAAATAGAA CTTAACGATA GTCTTAGTGG GGGCACTTTA 
AATATTGCTA GAGAGAGTAG CGGAGAATTG GTATCTGTAG TTCAAGAATT GAACCTGAAT 
GAAACTGTTA GTCTGAGTCA AGAGGTTTAC AATGTAACCA CTGAGGCGCT GCAACGAATA 
GCGACAGATG AAATAGATGG CTTAAGTTCC GCTATAATTG AGGTTAACGA AAGCTTACTT 
AACGAATCTG CATTTGATGA GATGCACTTT AAACTAGACG AAACGGAACT GGACGAATCG 
GAAATCATAG ACAGAATGCT TGAGGCTTCA TTTGACGAGT TAGACGAATT AGAACTTGAG 
AACACAGAAC TTGACGAAAG TCGACTTGAC GACGAACAAA AGAGCGAATG CCAATTTAAC 
GAAACCTATA ACGAGTTGGA CAGCATAGAT CTTGATGCCA CAGAACTTAA CGCCACTGGA 
CTTAACGAAA TTTCATATGA CCAGCTGGAG GAACTAGAAC TTGACGATAG CGACTCCGTA 
ATGCCGTTCA CACGGCGCAA GAGCATCGTT TACGAAACGC CACAACTTAA CCTGAGCATT 
TCCGAACTAA AAGTGAATTA CGAAACGCAA GTGGAGCCAG TAATTAAATC AGAGTCACTC 
AACGAAGCCA TTAAATCGAT GAACAACGAC GATGAAGGCG ACGAGGAGAT GCAGGCAGGC 
AGTGAAGAGA AGGTAGATGA AGAGGAAAAC AGTGTAACTG AGACCCAGAC ACATCGTCGT 
CGGTGCAAAG GACTTCCGGC GAATCCCGCT AATGATGATG AACGGCCGTC CACCAGCTGC 
AAGGCTCTTT CCGTTTTTGG CGAGGTGCGA ACTGTGGCAC CACAGGCGGC GGCGGCAGAG 
GCAGCTGCAG CAGTTGGTGG TGCAACGCGG GGCGGTTGGA CTACACTCGA GCACGTATTT 
GTCGCCTGCA CTGTGGGCCT GATTACACCG AACGATTTGC TCACTTTGTG CCTGATCGTA 
ATTGGTGTGA TTATCATTAT TGCAATTGCT CTCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189027.1
CDS	277..5895
Translation	MDRLRGNHSIGGSSQQLSGKQKWSFGRLFRKKKEAESASSSEEDRKAGFVPAQRQSASKPKGKHGKGSRGCSKFDHIVVSQRQEQAPPHPPQPQIDNEFFLPLEAIQPEPYYQNQPGYYQRSSNSLDRRLLMQPGVQGNGYQQPKSRSAQRGPKPPGAHSSEEELISLNSSTFSKYRSDESIHLGSAAGQSRRSRAARNERYYKRLSRDGEPSHGGAPVVAQQRYKTQPLPLSIYQPTNASTGYVQWQPQPQKNLQNAMQNDGKRSISYDSQIHLQNMNGRMQSKPLPPPPPPRDPLRRVHVGGSGKIGSSSGDLRPVSYAFDHNSRCASDDRIWQPPHYQSVHSLNSQPSSSIVSAPVSQQQPHHRRFITRSERNANPGKQSLPNGIDFHYVADATPRSRKPIHMMDAGRMDNTGVPASSGILRTSKSGLPTPSPAPQQPLAKPRSFSSSRLSELRTYPMPMYSEVQKPKRNAPPVNPSPNQDNIVCGSLHIKPTQEHGNQNYVEIRHRDVHKTNSMPHHYQNRRSGEDLPNKYEEYVAEKREQHQQAPPPVYPERKMSLTPAVAPPPPVYFPRKKPANLEEAINELEAIYKSLGLTEPAEPKQEPKLDISSKMRVPTPSDFEKFALAHADDYEDEDSPTGEPDPIRDDVAFRNLQLANLQHRPSEKQPPFGIPVGPIVPAPQSDYLHVEPVRVKKKSGSPDIIKDDLAVRSLRKDPPGPKSSFVYPMQKKQRAIRTQSANIYNLIQRDAAKPSGGDLKSYMELTRNLERAGSMSNLQGEDGSDTKDVPATLDLLRKLKAQDEELDLARKHHPIPFRHPSQGGAIAQLPERLSKEEIPKAAPVPAPRKSLTPEPMLDDALNKIAQDAQASSIKLSQELHELRKEALITAARPKLNAEQQRLEDELQQIEAVSEAAKRCGQMLLETLPDAGQEGQEPQDKPLRKLHKEGKLIRAIDEVSEAANAVCEKILKDIVTTEPPVVVQEALQVRQTTKTGPGGEQLVMPHLIKKLDPIQSDKIEHIARRCMRQLSELANNPDYDNLATCNMSNSTTNATGGAAADATLVCPVPETQAKSQTLEEIDKIMQECERQAKSSSSTTTTDDQRTTAPSLTSGSGSGSFPPNVSTTASSFSSSSDCLAKSSSPSRASRPLSSSITSFNPYSSSDYIKSQSSDCHAPSTDPIKTFSTTSYEVQSTPQSTTISTNTMSTSQSNTISSTTNPSVESKPSAPSVSPPPLQLTPVGDRRGRNRERGASCSSTSSPSQYNSSEELAAIFGIEEQPKQQRRSRQTHNATGNGDDPATTEAPSSPDSDSANANDTANANANTTNPAQLDSANCATNPVMNVQLDVTTSTSTSASTTHPTSNDNASTCSSHSYKLETIPEQDHEEVESTLKLSPNFNRIYASKIRIVMDRNEEASLLPEGAANVALTQFDSLSSLEETSSNPDSGNVSLTDYPLANHANSSLELQTAYNSRFQVREHYVSEVSISASESTDEEQTDTECLRDADRLNTEDFPPLPTEEELEESLREEIELNDSLSGGTLNIARESSGELVSVVQELNLNETVSLSQEVYNVTTEALQRIATDEIDGLSSAIIEVNESLLNESAFDEMHFKLDETELDESEIIDRMLEASFDELDELELENTELDESRLDDEQKSECQFNETYNELDSIDLDATELNATGLNEISYDQLEELELDDSDSVMPFTRRKSIVYETPQLNLSISELKVNYETQVEPVIKSESLNEAIKSMNNDDEGDEEMQAGSEEKVDEEENSVTETQTHRRRCKGLPANPANDDERPSTSCKALSVFGEVRTVAPQAAAAEAAAAVGGATRGGWTTLEHVFVACTVGLITPNDLLTLCLIVIGVIIIIAIALT

Target ORF information:

RefSeq Version	NM_001202098.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster supervillin, transcript variant AG (Svil), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202098.2

ORF Insert Sequence:

ATGGATCGGC TAAGGGGCAA TCACAGCATT GGAGGATCCT CGCAGCAGCT TTCTGGCAAA 
CAGAAATGGT CCTTCGGGCG ACTTTTTCGC AAGAAAAAGG AGGCGGAGAG TGCATCCTCC 
AGCGAAGAAG ATCGCAAGGC GGGATTTGTG CCTGCCCAGC GCCAGTCAGC CAGCAAGCCG 
AAGGGAAAAC ATGGCAAAGG ATCGCGAGGT TGCAGCAAAT TCGATCACAT TGTGGTGAGT 
CAGCGGCAAG AGCAGGCTCC ACCACATCCA CCCCAACCGC AAATCGACAA TGAGTTCTTC 
TTGCCCTTGG AGGCCATACA ACCGGAGCCC TACTATCAAA ATCAACCGGG CTACTATCAA 
CGATCGAGTA ACTCGCTGGA CAGGAGATTA CTAATGCAGC CTGGAGTTCA GGGAAATGGT 
TACCAGCAGC CCAAATCTCG ATCGGCGCAG CGAGGTCCCA AGCCACCAGG AGCTCACTCC 
AGCGAGGAGG AACTGATCTC GCTGAACTCC TCCACGTTCT CCAAATACCG CAGCGATGAG 
AGTATTCATT TGGGTTCCGC TGCTGGACAA AGTCGACGCA GTCGAGCGGC CCGCAATGAG 
CGTTACTACA AGAGATTGTC CCGGGATGGC GAACCGAGTC ATGGCGGAGC ACCCGTTGTA 
GCCCAGCAGC GATACAAAAC CCAGCCACTG CCTTTATCCA TTTACCAGCC AACGAACGCG 
TCCACTGGCT ATGTCCAGTG GCAACCGCAG CCCCAGAAGA ACCTGCAGAA TGCGATGCAG 
AACGATGGAA AGCGAAGCAT CAGCTACGAC AGTCAAATAC ACCTGCAGAA CATGAATGGG 
AGGATGCAGA GTAAGCCATT GCCACCGCCA CCGCCTCCAA GGGATCCACT GAGGAGAGTG 
CATGTTGGAG GTTCCGGGAA GATTGGATCC AGCTCTGGAG ACTTGCGTCC CGTGTCCTAT 
GCCTTCGATC ACAATAGTCG CTGTGCGTCG GATGATCGCA TTTGGCAGCC ACCCCACTAC 
CAGTCGGTGC ATTCGCTCAA CTCCCAGCCC AGTTCGTCAA TAGTCAGTGC ACCAGTGAGT 
CAACAGCAAC CGCACCACAG GAGATTCATA ACTCGATCCG AGAGGAACGC AAATCCTGGC 
AAGCAATCCC TGCCGAATGG TATTGATTTC CATTATGTGG CAGATGCAAC GCCACGCTCC 
AGGAAGCCCA TACACATGAT GGATGCAGGC AGGATGGACA ATACAGGAGT GCCAGCATCC 
AGTGGTATAT TGCGAACCTC TAAGAGTGGG CTGCCCACAC CAAGTCCTGC TCCCCAGCAG 
CCCTTGGCCA AACCGCGGTC GTTCTCCAGC TCGCGACTTA GTGAGCTGCG AACCTATCCC 
ATGCCCATGT ACTCCGAGGT GCAGAAACCT AAGAGGAATG CGCCACCAGT CAATCCCTCG 
CCGAATCAGG ATAACATAGT GTGTGGGAGT CTGCACATAA AACCCACACA AGAGCATGGA 
AATCAAAACT ATGTGGAGAT TCGTCATCGG GATGTGCACA AGACCAACTC AATGCCGCAC 
CATTATCAGA ATAGGCGATC TGGCGAGGAT TTACCCAACA AATATGAGGA GTACGTGGCA 
GAAAAGAGGG AGCAGCATCA ACAGGCACCA CCCCCAGTTT ATCCGGAGCG CAAAATGAGC 
TTGACACCAG CAGTGGCGCC ACCGCCTCCT GTTTACTTCC CTCGCAAGAA GCCTGCCAAT 
TTGGAGGAGG CCATCAACGA ACTGGAGGCC ATTTACAAGT CTCTAGGACT CACTGAACCA 
GCCGAGCCCA AACAGGAACC AAAGCTGGAT ATTTCCTCCA AGATGCGTGT GCCAACTCCC 
AGTGATTTTG AAAAGTTCGC CTTGGCGCAT GCTGATGACT ACGAGGATGA AGATTCGCCA 
ACCGGAGAAC CAGATCCAAT CAGAGATGAT GTGGCCTTCA GGAACCTACA GTTGGCCAAT 
CTGCAACATC GGCCATCGGA GAAGCAGCCT CCATTCGGCA TCCCAGTGGG ACCCATTGTG 
CCAGCTCCAC AGTCGGATTA TCTTCACGTA GAGCCAGTGA GGGTGAAGAA GAAGAGTGGC 
TCTCCTGACA TCATTAAAGA TGATCTTGCT GTGCGTTCAT TGAGAAAGGA TCCTCCTGGT 
CCCAAATCCA GCTTTGTCTA CCCAATGCAA AAGAAGCAAC GGGCTATTCG AACCCAATCG 
GCCAACATAT ACAATCTGAT CCAAAGAGAC GCTGCCAAGC CATCTGGTGG AGATCTTAAG 
AGCTACATGG AGCTGACTCG CAACCTGGAA AGAGCTGGCA GCATGTCCAA TCTGCAGGGT 
GAGGATGGCA GTGATACCAA AGATGTACCC GCCACACTGG ATCTTCTCCG AAAGTTGAAA 
GCTCAGGATG AGGAACTGGA TTTGGCTAGA AAGCATCACC CCATACCGTT TAGACATCCC 
AGTCAGGGAG GAGCTATTGC CCAACTGCCA GAACGGTTGT CCAAAGAGGA AATACCCAAG 
GCAGCGCCTG TGCCAGCTCC CCGAAAGAGT CTGACTCCTG AACCCATGCT AGATGATGCT 
CTCAACAAGA TTGCCCAGGA TGCGCAGGCC AGCAGCATTA AGCTGAGTCA GGAACTCCAT 
GAACTTCGCA AGGAGGCATT GATTACAGCT GCGAGACCAA AGTTGAATGC CGAACAACAG 
AGATTAGAGG ATGAACTGCA GCAGATAGAA GCTGTCTCCG AGGCAGCCAA ACGTTGTGGC 
CAAATGCTGC TCGAAACTCT GCCAGATGCG GGACAGGAGG GTCAGGAACC TCAGGATAAG 
CCCCTCAGGA AGCTACATAA GGAGGGCAAA CTCATACGAG CTATCGATGA GGTGTCCGAA 
GCTGCTAATG CGGTCTGTGA GAAGATCCTC AAGGACATTG TGACCACAGA ACCACCGGTG 
GTAGTTCAGG AGGCCCTCCA AGTGAGGCAG ACCACAAAGA CTGGACCAGG CGGAGAACAG 
CTGGTGATGC CGCATCTTAT CAAGAAACTA GATCCCATAC AATCCGACAA GATCGAGCAC 
ATTGCCAGAC GATGCATGCG GCAGTTGAGC GAACTGGCCA ATAATCCGGA CTACGACAAC 
CTGGCCACCT GTAATATGAG CAACAGCACC ACCAACGCAA CGGGTGGAGC AGCCGCTGAT 
GCCACTCTCG TTTGTCCCGT TCCAGAGACC CAAGCCAAGA GCCAGACGCT CGAGGAGATT 
GACAAGATCA TGCAGGAGTG CGAGCGTCAG GCGAAGAGCA GCAGCTCAAC CACGACCACC 
GATGATCAGC GCACCACTGC CCCCAGTCTG ACGAGTGGAA GTGGCAGCGG GAGCTTCCCA 
CCCAATGTGA GCACCACCGC CAGTTCGTTC AGCTCAAGCA GCGACTGTTT GGCCAAGTCG 
TCGAGTCCTT CGCGCGCCAG CCGTCCGCTC TCCTCGAGCA TCACCTCTTT CAATCCATAT 
TCCTCCAGCG ACTACATCAA GTCGCAGAGC AGTGATTGCC ATGCACCAAG CACCGATCCG 
ATCAAGACCT TTAGCACCAC CTCGTACGAG GTGCAGTCCA CACCGCAGAG CACCACCATT 
AGCACCAATA CGATGAGCAC GAGCCAGAGC AACACGATCA GTAGCACTAC TAATCCGAGT 
GTGGAGTCCA AGCCATCCGC CCCGTCGGTA TCGCCACCAC CCCTCCAGTT GACGCCAGTG 
GGTGATCGGA GGGGCAGGAA CCGGGAGCGG GGAGCTTCCT GCTCCTCCAC ATCCTCACCT 
TCACAGTACA ACTCGAGCGA GGAGCTGGCC GCCATTTTTG GCATCGAGGA GCAACCGAAA 
CAGCAGCGTC GCAGTCGGCA GACGCATAAT GCCACAGGTA ACGGAGATGA TCCCGCCACC 
ACTGAGGCCC CGAGTTCGCC TGATTCCGAT TCCGCCAACG CCAATGATAC TGCCAATGCC 
AATGCCAATA CTACTAATCC CGCCCAGCTC GATTCAGCTA ATTGCGCTAC TAATCCCGTT 
ATGAATGTTC AACTTGATGT TACTACTAGC ACCTCCACCT CAGCCTCAAC CACCCATCCA 
ACCTCTAACG ATAATGCTAG CACTTGTAGT AGCCATAGTT ATAAGCTAGA GACGATTCCG 
GAGCAGGATC ACGAGGAGGT GGAGTCCACG CTAAAGTTGT CGCCGAACTT CAATAGGATT 
TATGCCAGCA AGATTCGCAT AGTAATGGAT CGCAATGAAG AGGCAAGTCT CCTGCCGGAA 
GGAGCAGCTA ATGTTGCTTT AACTCAATTC GATTCTTTGA GTTCTCTGGA AGAGACCTCG 
TCCAATCCGG ACTCTGGGAA TGTTAGTCTC ACGGATTATC CCCTGGCTAA CCATGCGAAT 
TCCTCCCTGG AACTGCAGAC CGCTTACAAT AGTCGATTTC AAGTGAGGGA GCATTACGTA 
TCCGAGGTTT CCATTAGTGC TTCGGAATCA ACGGATGAGG AGCAGACGGA CACGGAGTGC 
TTAAGAGATG CTGATCGTTT GAACACAGAA GACTTTCCAC CTTTACCCAC AGAAGAAGAG 
CTAGAGGAGA GTCTACGGGA AGAAATAGAA CTTAACGATA GTCTTAGTGG GGGCACTTTA 
AATATTGCTA GAGAGAGTAG CGGAGAATTG GTATCTGTAG TTCAAGAATT GAACCTGAAT 
GAAACTGTTA GTCTGAGTCA AGAGGTTTAC AATGTAACCA CTGAGGCGCT GCAACGAATA 
GCGACAGATG AAATAGATGG CTTAAGTTCC GCTATAATTG AGGTTAACGA AAGCTTACTT 
AACGAATCTG CATTTGATGA GATGCACTTT AAACTAGACG AAACGGAACT GGACGAATCG 
GAAATCATAG ACAGAATGCT TGAGGCTTCA TTTGACGAGT TAGACGAATT AGAACTTGAG 
AACACAGAAC TTGACGAAAG TCGACTTGAC GACGAACAAA AGAGCGAATG CCAATTTAAC 
GAAACCTATA ACGAGTTGGA CAGCATAGAT CTTGATGCCA CAGAACTTAA CGCCACTGGA 
CTTAACGAAA TTTCATATGA CCAGCTGGAG GAACTAGAAC TTGACGATAG CGACTCCGTA 
ATGCCGTTCA CACGGCGCAA GAGCATCGTT TACGAAACGC CACAACTTAA CCTGAGCATT 
TCCGAACTAA AAGTGAATTA CGAAACGCAA GTGGAGCCAG TAATTAAATC AGAGTCACTC 
AACGAAGCCA TTAAATCGAT GAACAACGAC GATGAAGGCG ACGAGGAGAT GCAGGCAGGC 
AGTGAAGAGA AGGTAGATGA AGAGGAAAAC AGTGTAACTG AGACCCAGAC ACATCGTCGT 
CGGTGCAAAG GACTTCCGGC GAATCCCGCT AATGATGATG AACGGCCGTC CACCAGCTGC 
AAGGCTCTTT CCGTTTTTGG CGAGGTGCGA ACTGTGGCAC CACAGGCGGC GGCGGCAGAG 
GCAGCTGCAG CAGTTGGTGG TGCAACGCGG GGCGGTTGGA CTACACTCGA GCACGTATTT 
GTCGCCTGCA CTGTGGGCCT GATTACACCG AACGATTTGC TCACTTTGTG CCTGATCGTA 
ATTGGTGTGA TTATCATTAT TGCAATTGCT CTCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Svil ( NM_001169859.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163330.1 Drosophila melanogaster supervillin, transcript variant F (Svil), mRNA.

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CloneID	OFa19996
Clone ID Related Accession (Same CDS sequence)	NM_001169859.2
Accession Version	NM_001169859.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	supervillin, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001169859.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCCATG ACCAGCAGCG AGATCCTCCA GTTGAAGTTC CTAAGGCTCT AAACGTCGAG 
CCACCGCAGC CAGAAGACAT CGTCCTGGGC TCCTGCCCCA CTGCATCTGC CCACTCAGAT 
AAAAAGAAGG AAGAGGAGCA ACGCAATCGC TACAGTTCGC CCGTTTCACC AAAAGCCTCC 
AGCAACGGCG GAGTCCAAGC ACAACGTCGT CTTAGCAACT CGGAGCTATC GCCCAAGTCG 
CCAGGTGCCA GTGGATTCAC TTCGGAGCCA GTCGCGTCAG CCACAGCGAA CAGAGTCCCG 
ACGAGATCTG CCTCCCATCC TGTGGACACA CCGCCCACAC AAAGGCGACA GCCTCTGGTG 
GCCGTGAGTT CGGGCATCTC CATGAAGCAG AGTCTCAGCG AGCTGCACTT CACGGGTGGA 
GCAGTCAGTC CGCCCAAGGC TACGGCGGAA TGCACTCTAA GGATGCGACC ACGTAGCTCC 
ACGATGAGCA CCGAGAACAC GGAGAATGGC CGACGTGACC ACTTGGCTAA TCTGCAACTA 
AGTCCTGTGC AAAAGCAAAA GCGAACGCCC GAAGTATCAC CACGCCGTTT CGGAGATCGC 
GACAAGTCGG CCAAACGGAA ATCGAATTTG AATCGTTCGT TGACCGAGGA AGCCACCAAG 
GATGGCGATG AGGTTGCCTC TTCGACCCGC CGGCGCCGAC GTCGTGAACT GGGAATGGCT 
CGTCCCCTGG TTCCGGGCGA TGATGACGCC CTAAGAGATT TGCTAACGCC GCAGAAGAGT 
TCCTGCAGCG AGTCAACCAA CTCCATATCC GAACCCCATC TCCAGTTCAT ACCAGCCGTT 
CGTCATCCGG TGGTTCCAGT ACGCACCATG ACTGCTGTGC TGCCTGCACC ACGACCGCTG 
CATACTCCTG CGGCCCGCAA CGTCAGCCAG AAGTTCAATG AAAGGACCAA AACAGATCTG 
GCCGAGATCA AGAAGATTGC TGAGCAATCG GAGGCGCAGG TGCAACAGCC TTCGGTGGAT 
GATAACCAGA AAATGTCAGC CATAATGCGC AGGAAGAGCA CGGATGAGGG TGGATCAGCT 
TTGGGGGCAA CAAACCAGAA TTCACCACCT GCCCCAAACC AAATTGTATC CATACTGAAG 
AAGAAGGAAC ATGGACTTGG CGAGTGCAAC TCCAGTGCTT CAAGCAATCC CTCGCCGGTG 
ACCTTCTCTT CTAGCGTGGT GGATCATCCT TTGGCTGGAG CTGGCCGGTG CAAACGGCAG 
GGAATACTCA AGAAGAGATC GAGTCTAGAT GAATCGCGCT ACTACTCCCG ATCGCATTCA 
CCCGACGAAC GTAGTATTCT CATTAAGTCG GCCAGGAGGA ATTCCCTGGA GGAAGCTGGC 
ACTGGATTGA GTCCGGCTCA GGCCCATGGA ATCCTCAAGC AGAGCAGCTA CGACAGCAGC 
AAGAGCGACG GCTGCCCTTC GGCCACGGAG AGCCAGCCAC ACAGTATCCT GAAAAAGAAG 
GATTCGCTAT CGACACCTTC GGACGGAGGA TGCCACAAGC ATGTCTCTAT ATCGCAAGCT 
GTAACTTTAG CTGCTGCTGA ACTGGCTGCC CATGATGGAA TCACCTTCGA TGATGGTGAG 
GAGCATGAGA TACGACCGAT CCTAAAGCAG GAGAGCACCT CCAGCGAGGA GGCTGTGCGT 
CCGCCAAAGC CCATACTCAA AAAGAAATCC TTTGGCGAGG CGGATGAACA CGAAATACGT 
CCTATATTGA AGAGCTCTCG TAAAAGCAGT CGAGAGGAGT TCGATTTGAG TGGCTTGGAA 
AACGAGGTCA ACGATTCCCT ATCGTCAATC CTAAAGACAG ACTCGCCGTC CAAGCGTCGG 
TCGCTCGGTT CAGCTTTGCA TGATTTGGAG GAGTCCACCA CTTCCCCGAG CATGCTCAAG 
AGGCGCACCC GTTCGCTGGA ACGTCAAGAT GTCCAGCCTG GCATGGATTT GGCAGCTGCA 
CTGGATGCCA TCGCCACATC ACAGGCTACT CCCAGCACCC CGGTGGACTT CGTGACCACA 
CCAACAAGTA TATCGGTGGC GGAGCGGATC AAGCGCATGG AGATGCTGTC GACGCCCACT 
TCCCGTGCGG CGGGTGGGGC CAACAGCAGC TGGGAATCGC CACTGCAGGC ATCGTCAGCA 
GAGCATGGAG CACCCACCAC CCCAAGGGTT CTCAAGCCAA GTGTGCTGCG CAGAGATTTG 
CAGAGGGAGC GGTACAAAAC GCAACCCGTG ACCAACGAGG AGATGAGCTT CTTCAAAGCC 
AACTCCGCGC CCTTCGACAT CTCGGCTACA TCGGCCTTCA AGCCCACCAA GCCGCTGGAC 
ATCCGCTTGA CCCACGCACC GCATGCACCG CTGATTTCGC CGATCACCGG ACAGCCACTC 
AGCCACGTGC CCGCGCCACT GCTGCTATCC TCGTCGACGA ACTCGGGAGC GGGATCCTCG 
TTCCGTCTGG CCCGCAGCTC CACCCAACCG CTGCAGTCGC CGCGAGTTGT CCACCTGGCC 
GGTCAGGCTA CTGGTGGCTC CGCTTTGGCA CGCCAGAACT CGGTGAACGA CAGCATCAAT 
CTCAGCGAGC AGCTAACCCT ATCCATCGGC ACGGACAGCG AGCACATCTT TGAGATGTCC 
GCTTTGGAGG AGGACTCGGC TATTCAGTCG CTGAGCGACG AGACAGCCGC TTCCGCATCT 
GCCAACTCAA CATCCACGAC CACTGCAACC ACCATGACAC CGCCCAGTGG CCTGACGCGG 
AGCAACAGCG TTCGGGCCAG GGCCAATATG TTCCAGCAAT TGCAGGAGCA GTCGCGCAAC 
CAACGGGCAA CGGGAACCAC CAACTCCCGT CAATCTCCAC CAGCGTCGTC GGCGGAGGTG 
TCTTCAAGTG CAGCCGACAA TTCCATGCAC AGTGACGAGC GGTCCACGCC AAATGCAACG 
ATAGATGCGG AATTCGAACC CTCATCCCTG TCGCTGGCTG AGCGACTGGC CTTCTTCAGC 
AATCTGTGCG AGAGCGGCGG AGGAGGTGGG AGTACCAGTG CCGGTGGAAG GTACCGCAGT 
CGCACCAGCA GCTATTCAAG GAGTCCGCCC GGCGATCGCA CCACTCCCAG GAGCAGCACC 
ACCGCCAGCA GTGTCAGTAT GACGCCCACG CCCATGGATT ACTCTCCGAT TCCCCACGAC 
AAGGAGCCAT CTAGTCTCAC GCTGGAGAGC ATCATCGAGC CCGAGCAGGA GGAGGTCAAG 
GAGCAGGTGG ACCAGGATGA GGTCAAGTTG AGGAAGAAGG AGCCAGTCCA GAGGGAATCT 
TCCCAGCACA ATGGCTTCCA CGTGCTGACC AGGTCCGCCA CCGAACCGCC GTCGTCCACC 
TCGCCCCTGC GCATCAGCAT ACGGACTGTG GGAAAGCTAG TCCTGCCCGA TACCTTCCGC 
GGCGATCGCA ACAATAACAG CAGTAGTAGA AGTGGGAGCA ACAGCTGGAG CAACACTTGC 
ATGGTCAAGC TGCAGAGCTT GGAGCCCGTT AGCCTGTCCG TTCATTCGCG CACCATTGGT 
AAGGTTAAGT CGCCATTCAT TGAAAAGCAG CAGGAGAAAC CACACACCGA GAAGCTAGCG 
AACGGAACCA GCGACAGTGG CTCCGATTCG GGCAAGGAGA ACTGCGCCTC CAACGAGCAG 
GAACATCTGC AGCACCAGCA GTCGCAGGAC GAGGATCGAG CTCCGCCCAG GGTGTCCGAG 
ATGCGGAGGA AAATCAGCCG ACTGGTGCAA CAACAGCAGC CGCAACCACA GCCCGTCAAT 
CGGCGGCACA CCACCGAGAT CACCTCCGTG ACCGTTGGAA CGCGTTCGCC CAGTGTGGAC 
GATCGCTTTG CCAAGTACTT TGGTGTTAAG GAGAGCTGCA ACAGCACGAC CACCGTGCAT 
AAAACGCAAT CGCTGCCACC CAGCCAAGTT GAGGCCACCA ATGCGCACGT GAAACTACCG 
CAGATCACTA CCGTGGTTTC CAAACGGCGC GAGCTGCTCA AGAGGACGCG ACCCCTGTCC 
ATGGATCACT ACTCCAGCGG CTGCACCAGT CCCACAGCCA TGCCAAGTCC CAAGAGAGCA 
CATTCGCCCG TCGGCCGGCG GAAGGCCGCC ATTTCCAGCA TCGAGGACAT TGAGGTCACG 
CCGGATGAAC TCCGGGCCGC TGGCAGGAAC TTCAAGCTGT TGTACGGCGA GTTATTGGGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163330.1
CDS	1026..5228
Translation	MTHDQQRDPPVEVPKALNVEPPQPEDIVLGSCPTASAHSDKKKEEEQRNRYSSPVSPKASSNGGVQAQRRLSNSELSPKSPGASGFTSEPVASATANRVPTRSASHPVDTPPTQRRQPLVAVSSGISMKQSLSELHFTGGAVSPPKATAECTLRMRPRSSTMSTENTENGRRDHLANLQLSPVQKQKRTPEVSPRRFGDRDKSAKRKSNLNRSLTEEATKDGDEVASSTRRRRRRELGMARPLVPGDDDALRDLLTPQKSSCSESTNSISEPHLQFIPAVRHPVVPVRTMTAVLPAPRPLHTPAARNVSQKFNERTKTDLAEIKKIAEQSEAQVQQPSVDDNQKMSAIMRRKSTDEGGSALGATNQNSPPAPNQIVSILKKKEHGLGECNSSASSNPSPVTFSSSVVDHPLAGAGRCKRQGILKKRSSLDESRYYSRSHSPDERSILIKSARRNSLEEAGTGLSPAQAHGILKQSSYDSSKSDGCPSATESQPHSILKKKDSLSTPSDGGCHKHVSISQAVTLAAAELAAHDGITFDDGEEHEIRPILKQESTSSEEAVRPPKPILKKKSFGEADEHEIRPILKSSRKSSREEFDLSGLENEVNDSLSSILKTDSPSKRRSLGSALHDLEESTTSPSMLKRRTRSLERQDVQPGMDLAAALDAIATSQATPSTPVDFVTTPTSISVAERIKRMEMLSTPTSRAAGGANSSWESPLQASSAEHGAPTTPRVLKPSVLRRDLQRERYKTQPVTNEEMSFFKANSAPFDISATSAFKPTKPLDIRLTHAPHAPLISPITGQPLSHVPAPLLLSSSTNSGAGSSFRLARSSTQPLQSPRVVHLAGQATGGSALARQNSVNDSINLSEQLTLSIGTDSEHIFEMSALEEDSAIQSLSDETAASASANSTSTTTATTMTPPSGLTRSNSVRARANMFQQLQEQSRNQRATGTTNSRQSPPASSAEVSSSAADNSMHSDERSTPNATIDAEFEPSSLSLAERLAFFSNLCESGGGGGSTSAGGRYRSRTSSYSRSPPGDRTTPRSSTTASSVSMTPTPMDYSPIPHDKEPSSLTLESIIEPEQEEVKEQVDQDEVKLRKKEPVQRESSQHNGFHVLTRSATEPPSSTSPLRISIRTVGKLVLPDTFRGDRNNNSSSRSGSNSWSNTCMVKLQSLEPVSLSVHSRTIGKVKSPFIEKQQEKPHTEKLANGTSDSGSDSGKENCASNEQEHLQHQQSQDEDRAPPRVSEMRRKISRLVQQQQPQPQPVNRRHTTEITSVTVGTRSPSVDDRFAKYFGVKESCNSTTTVHKTQSLPPSQVEATNAHVKLPQITTVVSKRRELLKRTRPLSMDHYSSGCTSPTAMPSPKRAHSPVGRRKAAISSIEDIEVTPDELRAAGRNFKLLYGELLG

Target ORF information:

RefSeq Version	NM_001169859.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster supervillin, transcript variant F (Svil), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169859.2

ORF Insert Sequence:

ATGACCCATG ACCAGCAGCG AGATCCTCCA GTTGAAGTTC CTAAGGCTCT AAACGTCGAG 
CCACCGCAGC CAGAAGACAT CGTCCTGGGC TCCTGCCCCA CTGCATCTGC CCACTCAGAT 
AAAAAGAAGG AAGAGGAGCA ACGCAATCGC TACAGTTCGC CCGTTTCACC AAAAGCCTCC 
AGCAACGGCG GAGTCCAAGC ACAACGTCGT CTTAGCAACT CGGAGCTATC GCCCAAGTCG 
CCAGGTGCCA GTGGATTCAC TTCGGAGCCA GTCGCGTCAG CCACAGCGAA CAGAGTCCCG 
ACGAGATCTG CCTCCCATCC TGTGGACACA CCGCCCACAC AAAGGCGACA GCCTCTGGTG 
GCCGTGAGTT CGGGCATCTC CATGAAGCAG AGTCTCAGCG AGCTGCACTT CACGGGTGGA 
GCAGTCAGTC CGCCCAAGGC TACGGCGGAA TGCACTCTAA GGATGCGACC ACGTAGCTCC 
ACGATGAGCA CCGAGAACAC GGAGAATGGC CGACGTGACC ACTTGGCTAA TCTGCAACTA 
AGTCCTGTGC AAAAGCAAAA GCGAACGCCC GAAGTATCAC CACGCCGTTT CGGAGATCGC 
GACAAGTCGG CCAAACGGAA ATCGAATTTG AATCGTTCGT TGACCGAGGA AGCCACCAAG 
GATGGCGATG AGGTTGCCTC TTCGACCCGC CGGCGCCGAC GTCGTGAACT GGGAATGGCT 
CGTCCCCTGG TTCCGGGCGA TGATGACGCC CTAAGAGATT TGCTAACGCC GCAGAAGAGT 
TCCTGCAGCG AGTCAACCAA CTCCATATCC GAACCCCATC TCCAGTTCAT ACCAGCCGTT 
CGTCATCCGG TGGTTCCAGT ACGCACCATG ACTGCTGTGC TGCCTGCACC ACGACCGCTG 
CATACTCCTG CGGCCCGCAA CGTCAGCCAG AAGTTCAATG AAAGGACCAA AACAGATCTG 
GCCGAGATCA AGAAGATTGC TGAGCAATCG GAGGCGCAGG TGCAACAGCC TTCGGTGGAT 
GATAACCAGA AAATGTCAGC CATAATGCGC AGGAAGAGCA CGGATGAGGG TGGATCAGCT 
TTGGGGGCAA CAAACCAGAA TTCACCACCT GCCCCAAACC AAATTGTATC CATACTGAAG 
AAGAAGGAAC ATGGACTTGG CGAGTGCAAC TCCAGTGCTT CAAGCAATCC CTCGCCGGTG 
ACCTTCTCTT CTAGCGTGGT GGATCATCCT TTGGCTGGAG CTGGCCGGTG CAAACGGCAG 
GGAATACTCA AGAAGAGATC GAGTCTAGAT GAATCGCGCT ACTACTCCCG ATCGCATTCA 
CCCGACGAAC GTAGTATTCT CATTAAGTCG GCCAGGAGGA ATTCCCTGGA GGAAGCTGGC 
ACTGGATTGA GTCCGGCTCA GGCCCATGGA ATCCTCAAGC AGAGCAGCTA CGACAGCAGC 
AAGAGCGACG GCTGCCCTTC GGCCACGGAG AGCCAGCCAC ACAGTATCCT GAAAAAGAAG 
GATTCGCTAT CGACACCTTC GGACGGAGGA TGCCACAAGC ATGTCTCTAT ATCGCAAGCT 
GTAACTTTAG CTGCTGCTGA ACTGGCTGCC CATGATGGAA TCACCTTCGA TGATGGTGAG 
GAGCATGAGA TACGACCGAT CCTAAAGCAG GAGAGCACCT CCAGCGAGGA GGCTGTGCGT 
CCGCCAAAGC CCATACTCAA AAAGAAATCC TTTGGCGAGG CGGATGAACA CGAAATACGT 
CCTATATTGA AGAGCTCTCG TAAAAGCAGT CGAGAGGAGT TCGATTTGAG TGGCTTGGAA 
AACGAGGTCA ACGATTCCCT ATCGTCAATC CTAAAGACAG ACTCGCCGTC CAAGCGTCGG 
TCGCTCGGTT CAGCTTTGCA TGATTTGGAG GAGTCCACCA CTTCCCCGAG CATGCTCAAG 
AGGCGCACCC GTTCGCTGGA ACGTCAAGAT GTCCAGCCTG GCATGGATTT GGCAGCTGCA 
CTGGATGCCA TCGCCACATC ACAGGCTACT CCCAGCACCC CGGTGGACTT CGTGACCACA 
CCAACAAGTA TATCGGTGGC GGAGCGGATC AAGCGCATGG AGATGCTGTC GACGCCCACT 
TCCCGTGCGG CGGGTGGGGC CAACAGCAGC TGGGAATCGC CACTGCAGGC ATCGTCAGCA 
GAGCATGGAG CACCCACCAC CCCAAGGGTT CTCAAGCCAA GTGTGCTGCG CAGAGATTTG 
CAGAGGGAGC GGTACAAAAC GCAACCCGTG ACCAACGAGG AGATGAGCTT CTTCAAAGCC 
AACTCCGCGC CCTTCGACAT CTCGGCTACA TCGGCCTTCA AGCCCACCAA GCCGCTGGAC 
ATCCGCTTGA CCCACGCACC GCATGCACCG CTGATTTCGC CGATCACCGG ACAGCCACTC 
AGCCACGTGC CCGCGCCACT GCTGCTATCC TCGTCGACGA ACTCGGGAGC GGGATCCTCG 
TTCCGTCTGG CCCGCAGCTC CACCCAACCG CTGCAGTCGC CGCGAGTTGT CCACCTGGCC 
GGTCAGGCTA CTGGTGGCTC CGCTTTGGCA CGCCAGAACT CGGTGAACGA CAGCATCAAT 
CTCAGCGAGC AGCTAACCCT ATCCATCGGC ACGGACAGCG AGCACATCTT TGAGATGTCC 
GCTTTGGAGG AGGACTCGGC TATTCAGTCG CTGAGCGACG AGACAGCCGC TTCCGCATCT 
GCCAACTCAA CATCCACGAC CACTGCAACC ACCATGACAC CGCCCAGTGG CCTGACGCGG 
AGCAACAGCG TTCGGGCCAG GGCCAATATG TTCCAGCAAT TGCAGGAGCA GTCGCGCAAC 
CAACGGGCAA CGGGAACCAC CAACTCCCGT CAATCTCCAC CAGCGTCGTC GGCGGAGGTG 
TCTTCAAGTG CAGCCGACAA TTCCATGCAC AGTGACGAGC GGTCCACGCC AAATGCAACG 
ATAGATGCGG AATTCGAACC CTCATCCCTG TCGCTGGCTG AGCGACTGGC CTTCTTCAGC 
AATCTGTGCG AGAGCGGCGG AGGAGGTGGG AGTACCAGTG CCGGTGGAAG GTACCGCAGT 
CGCACCAGCA GCTATTCAAG GAGTCCGCCC GGCGATCGCA CCACTCCCAG GAGCAGCACC 
ACCGCCAGCA GTGTCAGTAT GACGCCCACG CCCATGGATT ACTCTCCGAT TCCCCACGAC 
AAGGAGCCAT CTAGTCTCAC GCTGGAGAGC ATCATCGAGC CCGAGCAGGA GGAGGTCAAG 
GAGCAGGTGG ACCAGGATGA GGTCAAGTTG AGGAAGAAGG AGCCAGTCCA GAGGGAATCT 
TCCCAGCACA ATGGCTTCCA CGTGCTGACC AGGTCCGCCA CCGAACCGCC GTCGTCCACC 
TCGCCCCTGC GCATCAGCAT ACGGACTGTG GGAAAGCTAG TCCTGCCCGA TACCTTCCGC 
GGCGATCGCA ACAATAACAG CAGTAGTAGA AGTGGGAGCA ACAGCTGGAG CAACACTTGC 
ATGGTCAAGC TGCAGAGCTT GGAGCCCGTT AGCCTGTCCG TTCATTCGCG CACCATTGGT 
AAGGTTAAGT CGCCATTCAT TGAAAAGCAG CAGGAGAAAC CACACACCGA GAAGCTAGCG 
AACGGAACCA GCGACAGTGG CTCCGATTCG GGCAAGGAGA ACTGCGCCTC CAACGAGCAG 
GAACATCTGC AGCACCAGCA GTCGCAGGAC GAGGATCGAG CTCCGCCCAG GGTGTCCGAG 
ATGCGGAGGA AAATCAGCCG ACTGGTGCAA CAACAGCAGC CGCAACCACA GCCCGTCAAT 
CGGCGGCACA CCACCGAGAT CACCTCCGTG ACCGTTGGAA CGCGTTCGCC CAGTGTGGAC 
GATCGCTTTG CCAAGTACTT TGGTGTTAAG GAGAGCTGCA ACAGCACGAC CACCGTGCAT 
AAAACGCAAT CGCTGCCACC CAGCCAAGTT GAGGCCACCA ATGCGCACGT GAAACTACCG 
CAGATCACTA CCGTGGTTTC CAAACGGCGC GAGCTGCTCA AGAGGACGCG ACCCCTGTCC 
ATGGATCACT ACTCCAGCGG CTGCACCAGT CCCACAGCCA TGCCAAGTCC CAAGAGAGCA 
CATTCGCCCG TCGGCCGGCG GAAGGCCGCC ATTTCCAGCA TCGAGGACAT TGAGGTCACG 
CCGGATGAAC TCCGGGCCGC TGGCAGGAAC TTCAAGCTGT TGTACGGCGA GTTATTGGGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Svil ( NM_206246.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995968.2 Drosophila melanogaster supervillin, transcript variant X (Svil), mRNA.

Price & Availability↑

CloneID	OFa21979
Clone ID Related Accession (Same CDS sequence)	NM_206246.3
Accession Version	NM_206246.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4545bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	supervillin, isoform X
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_206246.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCGCTT TGGAGGAGGA CTCGGCTATT CAGTCGCTGA GCGACGAGAC AGCCGCTTCC 
GCATCTGCCA ACTCAACATC CACGACCACT GCAACCACCA TGACACCGCC CAGTGGCCTG 
ACGCGGAGCA ACAGCGTTCG GGCCAGGGCC AATATGTTCC AGCAATTGCA GGAGCAGTCG 
CGCAACCAAC GGGCAACGGG AACCACCAAC TCCCGTCAAT CTCCACCAGC GTCGTCGGCG 
GAGGTGTCTT CAAGTGCAGC CGACAATTCC ATGCACAGTG ACGAGCGGTC CACGCCAAAT 
GCAACGATAG ATGCGGAATT CGGAAAACCC AGCACCGAAC CACTTAAGAG CATTCTTAAG 
ACGGGAATGC CAGTGATGGG TATGGGACGC GGTTTAATGC CTGTGGATCT CAATGCCGAG 
CTGAAGAACC GGCTCAAACG CTCCACACAT GCCACTGTTT CCAATCTCCG CAAATCTGCC 
ACCTCGGCAG ATGCCACTAG AGCAGCTAGT GATGAGGTGG ACAATCCGCA GAGGAATTTA 
GCCCAGATCC TGAGGAATGT TTCCAAGGAG AACTCCACGC CGAAGCACGA TGATGCCGTG 
AGCCTAGTGC GGAATCTCTC TACTTTGGGA CAACCTCGAT CGCTGGCCCG TGACGATGCA 
GCCGGGAATT GTGCCTCGGC ATCCGAAGGC GAGAGTTCGG GGGGCCGCGA GATCGAGGCC 
ATAATCAAGA ACAGCGCTGT GGCCAGACGT CGCCGGCAGC AACAGCAGCA GTCGCAGCAG 
CAGAATCAGC CAGATGGCAA TCTCGTTGCG AAAAGCAAAA GTCACTCGGG CATATCAGCA 
CCCATCGGCC TTGGCCCCCA GCTACCACCC CTTCCCCTCG GCGGCGGTTT TGTTAAGTTG 
CGTCATGTAG CCAAGGAGGA GGATACCAGT AGCAGCAGCA ACACATCCTC CGCCAAGGAA 
TCGCCAGATA AGAAGGATCT TCGCCAGGAG CAGGCAGAAG CTTCGGGAGA CTCATCGCAC 
CCAACGGCTC TGCCACAGTT TCTTCAAGGC GTCCAACGAT CATCAACCGA GGTCATGGGC 
CCCGATCAGC GGTCTTTGGT GATTTCGAAG ACCGGCTCCA TTGCGGAGCG TTTGGCGGCA 
CTCCACAAAA GTGGCGAGGA CGACTGGAAG AAGCGCATCT CGAAGCGCGA CGAAGTCGAC 
GATGTGCACC GTGAAAACTT CGTAAACGAA TCGCTATCTT TGGCCCACTC GCTGTCGGAC 
AAACCACTGC CCCCATCACC TTTGATACCC ACCAGCTTGG AGGGCGGCAA GGTCTCCGAT 
CGTTTGAGCA AACTCAAGTG CAACTCGGAG AACTGGAAGC AGCGCATAGA GCAAACCGAT 
GCCAAGAAAT TCACAGTCGC CGGTCGACTG CAAAAGAAGG CCCAATCCCC CGTGGAGCTT 
CAATTCGAGC GTTCTGCCAA TGATGCGGCC AAAAAGTGTC CCATGCTGGA GGTGCGCAGC 
GCCAATCAGC CGCAGCTTGG GCTGGCCAAG AGCCCCTCCA TGATGGTCAC CTCTAGCACC 
AGCACCAATA GCACCAGCAA GTCCGCACTC CGCAGCCTGA GCGTCAATCC CGAGGAGGAG 
GGACCCACCA GCCTGAGTCG CTCCAACAGC AGCAGTTCGG AGTCCGATGG AGAGCGCAAA 
TCCCCCCAGA ATGCCAAGCA GAACAAGGAG CTGGCCAACA ACAAGAGTGC TGCAGCACCC 
ACCAATGTGG GAGCCAGGAT TCAGGTGCCC CGGCTGGACG ACAAGGAAAC TTTCGAGAAC 
TTCTTCTCCT CCAAGCCGAA GAAGGAGGAG AACGTTCAGC TGGAGATCAG CGCTTTCGAT 
GACATCAAGC CCACTGAGCG CTTGGTAAGC AAGCGACACG TACAGGGTCC CAAAGGTCGA 
CGGGCTGCAC GCAATCCTCT CAAGAGTTTA GCTGCTCGTG AGGACATTAG CTCCGAGTAC 
ACCGAAATGA GGTCGGGCAT AGCCGAGCGA GAATTACGGC GCCTAAAGTT GGAGTCATAT 
GGTCATAATG CCAACTTGGC TGCAGAGGCC ATAGCCGGAT TAGCCTCCGT CGAGGACTTC 
AAGTCGGTGG CACTGCGCTC CTCGTCGATT CCACTGCAGC AAATGTGGCT ACCCCACAAG 
CCAGTGATGT TACTCCATGT TAAGGGGCGC ACACATGTCC AAACCCGACT GGTGGAACCC 
GTGCATACCT CCCTGAACCG TGGTGATTGC TTCATCCTGG TGGCCGGATC GCAGCTTTTC 
CGCTACGTTG GATCCTTTGC AAACGTAATC GAGATCTCCC GGAGCAAGAA GATCTGCGCT 
GCCATAGTGG AAAACAAGGA TCTCGGTTGC ACCGCCACCC AGGAGGTGAT CCTCACGGAT 
GGCAAGTATT TGAAGGAGCG CCAGTGGCGT CAGTTCTGGC AGCTTTTGGG CAAAAAAGAG 
GATGATCAAT CGGATATAGC TGAATGCGGT CATGCCGATG AAGACGACGT GTTCGAGAGC 
AGCCTGATCG AGACTAACAA GATCTACGAG TACCAGGATG AGGCGCTCAT TCCGTTGGAG 
AAATTCTGGG GCTGCATCCC CAAGGTGGAC ATGTTGGACA CGCGTAAAGT TTTGGTCTTT 
GACTTTGGCA GCGAGCTGTA TGTGTGGAAT GGCAAGAATG CCCCTTCCGA TGCCAAGCGA 
GCTGCCATGA GATTGGCCCA GGAACACTTT GGTGCTGAGA ATTCGGCGGA CTATGCCGAC 
TGTTACTTGA ATCCCCTGAA CTATGTCGCC ATAGTGGGTC GTCGTGAGAA CACCAAGTAT 
GCCAAGAGGT GCGCACAGCG TCCAGAATGG TGTGTTCTCG GCAAGATCAC CCAGAACATG 
GAGACGGTGC TGTTCAAGGA GAAGTTCAGT GATTGGCCAG AGATAGAACG CGAGGATCTG 
GAGAAGGATT ACCTATCAAA CGGAGTGCAT ATGGTGCGTG CTTTAAACGG CACCGCTTTG 
CATAAGGGAG AACCCTACCA GGAACCAAAT CTGGTGCTCG AGCAGGCCAA CTTGGGGCGT 
GGCAATTTCT ACTACGACAC GGACACCATG CGGCACTTCG ATGTGATTAC CAAGTCAACG 
GACAAATGGC AGATCCACGA GTTCAACTTT GATGCGGCGA ATAATCGCGA TGACTACGGT 
CACTTCTACT CGGCGGAGAG CTATATAGTG CGCTGGATCT ACCAGATTTC GGTCACAGTT 
CGCGAACTCA GTGGCAAGGT GTCGAACAGA AGCACAGTGG GTCGAGACAG GTGTGTCTAT 
TTCACCTGGC AAGGACAGGA CTCAAGTGCC AACGAGAAGG GTGCAGCTGC TTTGCTGACC 
GTAGAGTTGG ACAAGGAGAA AGGCGCACAG ATGCGGGTGT CGCAAGGAGA TGAGTGCACA 
GCCTTCGTCC GCCTGTTCCG GCAAATGTGG CAACATCGTG GACGCAAGGA GCAATGTCTG 
GAGAGGCACA GCGAGTGGAG GCTCTACCAA CTGCAGGGCA ACGTCAGCGA GGAGACCATC 
ATCAAGGAAG TCGTCTGCCA GTCCAGCCAT TTGCGATCCC GCAGCTCCAT GCTGTTAATT 
CACGGCAGTC AGGGAAATGT GATTGTCTGG CATGGATCGA AGAGTGCTCC TCATACGCGT 
AATGTGGCTG TAGGAGTTGC TCAGGATCTG ATCAAGACGG TGCCTAAAGA TCTCTTCAGC 
GCCGAAACTG CCAACCTAAC TGAGGTGGAG GAGGGTTCCG ACGACGATCA GTGCAAGAAG 
GCCCTGGGAT TGAAGGATGA AAGAAATGAA TATGGCAGCC TGCTTAAGTC CACCAAGTCG 
TTCGATTACC AAATAAGGAT CTTCAACTTC TCCAGCACTC AGGGTGTTTT CAAAGCCCTC 
GAACTGAGCG ATCCGCTGCG CTGCCAGGAT CTGCATAGTC CGTATCCCTT CAGCCAGTCG 
CAGCTGTACA ATGCAAGGCA GCCGACTATC TTTTTGCTGG ACGACGGCGA TGAGTTGTGG 
CTCTGGATGG GATGGTGGCC TCTGGAAGAT GTGAAGATAA ACACCGATGA GCGAAGCAGT 
CCCACCAACG AAAATCGCGC TGGAGTAAAT CGCTGGATCT CAGAGAGACG TGCTGCCCTC 
GAGACAGCCG TTGACTATTG GCGTGCCAAG CACGGCGAGA ACGAGAATGA ACCCTTCCAT 
GGCATCAAAG GCCAGGTTGT GTGGGCAGGC CTGGAGCCAC TGGCCTTTAA GGCGCTGTTT 
CCCGACTGGA CAGAACGTCC GGATGTAAGA GAAATCAACG AAGAAGATGG GCGAACTGAT 
GCTTCCATAT CGATCGCCAC AATGCTGGCA CAAATGACGC AGACCGAGTA TCCCCTGGAA 
GTCCTGAAAG CCCGACCGCT GCCCGAAGGT GTGGAGCCCA CCCGCCTGGA GGTCTACCTT 
AATGCGGAGG ACTTCCAGGC GGCACTGGGA TGCAGCCGGG CGGAATTCGA GCAGCTGCCC 
ATCTGGAAAC AGACCAAGCT GAAGAAGGAG CGAGGGCTGT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995968.2
CDS	738..5282
Translation	MSALEEDSAIQSLSDETAASASANSTSTTTATTMTPPSGLTRSNSVRARANMFQQLQEQSRNQRATGTTNSRQSPPASSAEVSSSAADNSMHSDERSTPNATIDAEFGKPSTEPLKSILKTGMPVMGMGRGLMPVDLNAELKNRLKRSTHATVSNLRKSATSADATRAASDEVDNPQRNLAQILRNVSKENSTPKHDDAVSLVRNLSTLGQPRSLARDDAAGNCASASEGESSGGREIEAIIKNSAVARRRRQQQQQSQQQNQPDGNLVAKSKSHSGISAPIGLGPQLPPLPLGGGFVKLRHVAKEEDTSSSSNTSSAKESPDKKDLRQEQAEASGDSSHPTALPQFLQGVQRSSTEVMGPDQRSLVISKTGSIAERLAALHKSGEDDWKKRISKRDEVDDVHRENFVNESLSLAHSLSDKPLPPSPLIPTSLEGGKVSDRLSKLKCNSENWKQRIEQTDAKKFTVAGRLQKKAQSPVELQFERSANDAAKKCPMLEVRSANQPQLGLAKSPSMMVTSSTSTNSTSKSALRSLSVNPEEEGPTSLSRSNSSSSESDGERKSPQNAKQNKELANNKSAAAPTNVGARIQVPRLDDKETFENFFSSKPKKEENVQLEISAFDDIKPTERLVSKRHVQGPKGRRAARNPLKSLAAREDISSEYTEMRSGIAERELRRLKLESYGHNANLAAEAIAGLASVEDFKSVALRSSSIPLQQMWLPHKPVMLLHVKGRTHVQTRLVEPVHTSLNRGDCFILVAGSQLFRYVGSFANVIEISRSKKICAAIVENKDLGCTATQEVILTDGKYLKERQWRQFWQLLGKKEDDQSDIAECGHADEDDVFESSLIETNKIYEYQDEALIPLEKFWGCIPKVDMLDTRKVLVFDFGSELYVWNGKNAPSDAKRAAMRLAQEHFGAENSADYADCYLNPLNYVAIVGRRENTKYAKRCAQRPEWCVLGKITQNMETVLFKEKFSDWPEIEREDLEKDYLSNGVHMVRALNGTALHKGEPYQEPNLVLEQANLGRGNFYYDTDTMRHFDVITKSTDKWQIHEFNFDAANNRDDYGHFYSAESYIVRWIYQISVTVRELSGKVSNRSTVGRDRCVYFTWQGQDSSANEKGAAALLTVELDKEKGAQMRVSQGDECTAFVRLFRQMWQHRGRKEQCLERHSEWRLYQLQGNVSEETIIKEVVCQSSHLRSRSSMLLIHGSQGNVIVWHGSKSAPHTRNVAVGVAQDLIKTVPKDLFSAETANLTEVEEGSDDDQCKKALGLKDERNEYGSLLKSTKSFDYQIRIFNFSSTQGVFKALELSDPLRCQDLHSPYPFSQSQLYNARQPTIFLLDDGDELWLWMGWWPLEDVKINTDERSSPTNENRAGVNRWISERRAALETAVDYWRAKHGENENEPFHGIKGQVVWAGLEPLAFKALFPDWTERPDVREINEEDGRTDASISIATMLAQMTQTEYPLEVLKARPLPEGVEPTRLEVYLNAEDFQAALGCSRAEFEQLPIWKQTKLKKERGLF

Target ORF information:

RefSeq Version	NM_206246.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster supervillin, transcript variant X (Svil), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206246.3

ORF Insert Sequence:

ATGTCCGCTT TGGAGGAGGA CTCGGCTATT CAGTCGCTGA GCGACGAGAC AGCCGCTTCC 
GCATCTGCCA ACTCAACATC CACGACCACT GCAACCACCA TGACACCGCC CAGTGGCCTG 
ACGCGGAGCA ACAGCGTTCG GGCCAGGGCC AATATGTTCC AGCAATTGCA GGAGCAGTCG 
CGCAACCAAC GGGCAACGGG AACCACCAAC TCCCGTCAAT CTCCACCAGC GTCGTCGGCG 
GAGGTGTCTT CAAGTGCAGC CGACAATTCC ATGCACAGTG ACGAGCGGTC CACGCCAAAT 
GCAACGATAG ATGCGGAATT CGGAAAACCC AGCACCGAAC CACTTAAGAG CATTCTTAAG 
ACGGGAATGC CAGTGATGGG TATGGGACGC GGTTTAATGC CTGTGGATCT CAATGCCGAG 
CTGAAGAACC GGCTCAAACG CTCCACACAT GCCACTGTTT CCAATCTCCG CAAATCTGCC 
ACCTCGGCAG ATGCCACTAG AGCAGCTAGT GATGAGGTGG ACAATCCGCA GAGGAATTTA 
GCCCAGATCC TGAGGAATGT TTCCAAGGAG AACTCCACGC CGAAGCACGA TGATGCCGTG 
AGCCTAGTGC GGAATCTCTC TACTTTGGGA CAACCTCGAT CGCTGGCCCG TGACGATGCA 
GCCGGGAATT GTGCCTCGGC ATCCGAAGGC GAGAGTTCGG GGGGCCGCGA GATCGAGGCC 
ATAATCAAGA ACAGCGCTGT GGCCAGACGT CGCCGGCAGC AACAGCAGCA GTCGCAGCAG 
CAGAATCAGC CAGATGGCAA TCTCGTTGCG AAAAGCAAAA GTCACTCGGG CATATCAGCA 
CCCATCGGCC TTGGCCCCCA GCTACCACCC CTTCCCCTCG GCGGCGGTTT TGTTAAGTTG 
CGTCATGTAG CCAAGGAGGA GGATACCAGT AGCAGCAGCA ACACATCCTC CGCCAAGGAA 
TCGCCAGATA AGAAGGATCT TCGCCAGGAG CAGGCAGAAG CTTCGGGAGA CTCATCGCAC 
CCAACGGCTC TGCCACAGTT TCTTCAAGGC GTCCAACGAT CATCAACCGA GGTCATGGGC 
CCCGATCAGC GGTCTTTGGT GATTTCGAAG ACCGGCTCCA TTGCGGAGCG TTTGGCGGCA 
CTCCACAAAA GTGGCGAGGA CGACTGGAAG AAGCGCATCT CGAAGCGCGA CGAAGTCGAC 
GATGTGCACC GTGAAAACTT CGTAAACGAA TCGCTATCTT TGGCCCACTC GCTGTCGGAC 
AAACCACTGC CCCCATCACC TTTGATACCC ACCAGCTTGG AGGGCGGCAA GGTCTCCGAT 
CGTTTGAGCA AACTCAAGTG CAACTCGGAG AACTGGAAGC AGCGCATAGA GCAAACCGAT 
GCCAAGAAAT TCACAGTCGC CGGTCGACTG CAAAAGAAGG CCCAATCCCC CGTGGAGCTT 
CAATTCGAGC GTTCTGCCAA TGATGCGGCC AAAAAGTGTC CCATGCTGGA GGTGCGCAGC 
GCCAATCAGC CGCAGCTTGG GCTGGCCAAG AGCCCCTCCA TGATGGTCAC CTCTAGCACC 
AGCACCAATA GCACCAGCAA GTCCGCACTC CGCAGCCTGA GCGTCAATCC CGAGGAGGAG 
GGACCCACCA GCCTGAGTCG CTCCAACAGC AGCAGTTCGG AGTCCGATGG AGAGCGCAAA 
TCCCCCCAGA ATGCCAAGCA GAACAAGGAG CTGGCCAACA ACAAGAGTGC TGCAGCACCC 
ACCAATGTGG GAGCCAGGAT TCAGGTGCCC CGGCTGGACG ACAAGGAAAC TTTCGAGAAC 
TTCTTCTCCT CCAAGCCGAA GAAGGAGGAG AACGTTCAGC TGGAGATCAG CGCTTTCGAT 
GACATCAAGC CCACTGAGCG CTTGGTAAGC AAGCGACACG TACAGGGTCC CAAAGGTCGA 
CGGGCTGCAC GCAATCCTCT CAAGAGTTTA GCTGCTCGTG AGGACATTAG CTCCGAGTAC 
ACCGAAATGA GGTCGGGCAT AGCCGAGCGA GAATTACGGC GCCTAAAGTT GGAGTCATAT 
GGTCATAATG CCAACTTGGC TGCAGAGGCC ATAGCCGGAT TAGCCTCCGT CGAGGACTTC 
AAGTCGGTGG CACTGCGCTC CTCGTCGATT CCACTGCAGC AAATGTGGCT ACCCCACAAG 
CCAGTGATGT TACTCCATGT TAAGGGGCGC ACACATGTCC AAACCCGACT GGTGGAACCC 
GTGCATACCT CCCTGAACCG TGGTGATTGC TTCATCCTGG TGGCCGGATC GCAGCTTTTC 
CGCTACGTTG GATCCTTTGC AAACGTAATC GAGATCTCCC GGAGCAAGAA GATCTGCGCT 
GCCATAGTGG AAAACAAGGA TCTCGGTTGC ACCGCCACCC AGGAGGTGAT CCTCACGGAT 
GGCAAGTATT TGAAGGAGCG CCAGTGGCGT CAGTTCTGGC AGCTTTTGGG CAAAAAAGAG 
GATGATCAAT CGGATATAGC TGAATGCGGT CATGCCGATG AAGACGACGT GTTCGAGAGC 
AGCCTGATCG AGACTAACAA GATCTACGAG TACCAGGATG AGGCGCTCAT TCCGTTGGAG 
AAATTCTGGG GCTGCATCCC CAAGGTGGAC ATGTTGGACA CGCGTAAAGT TTTGGTCTTT 
GACTTTGGCA GCGAGCTGTA TGTGTGGAAT GGCAAGAATG CCCCTTCCGA TGCCAAGCGA 
GCTGCCATGA GATTGGCCCA GGAACACTTT GGTGCTGAGA ATTCGGCGGA CTATGCCGAC 
TGTTACTTGA ATCCCCTGAA CTATGTCGCC ATAGTGGGTC GTCGTGAGAA CACCAAGTAT 
GCCAAGAGGT GCGCACAGCG TCCAGAATGG TGTGTTCTCG GCAAGATCAC CCAGAACATG 
GAGACGGTGC TGTTCAAGGA GAAGTTCAGT GATTGGCCAG AGATAGAACG CGAGGATCTG 
GAGAAGGATT ACCTATCAAA CGGAGTGCAT ATGGTGCGTG CTTTAAACGG CACCGCTTTG 
CATAAGGGAG AACCCTACCA GGAACCAAAT CTGGTGCTCG AGCAGGCCAA CTTGGGGCGT 
GGCAATTTCT ACTACGACAC GGACACCATG CGGCACTTCG ATGTGATTAC CAAGTCAACG 
GACAAATGGC AGATCCACGA GTTCAACTTT GATGCGGCGA ATAATCGCGA TGACTACGGT 
CACTTCTACT CGGCGGAGAG CTATATAGTG CGCTGGATCT ACCAGATTTC GGTCACAGTT 
CGCGAACTCA GTGGCAAGGT GTCGAACAGA AGCACAGTGG GTCGAGACAG GTGTGTCTAT 
TTCACCTGGC AAGGACAGGA CTCAAGTGCC AACGAGAAGG GTGCAGCTGC TTTGCTGACC 
GTAGAGTTGG ACAAGGAGAA AGGCGCACAG ATGCGGGTGT CGCAAGGAGA TGAGTGCACA 
GCCTTCGTCC GCCTGTTCCG GCAAATGTGG CAACATCGTG GACGCAAGGA GCAATGTCTG 
GAGAGGCACA GCGAGTGGAG GCTCTACCAA CTGCAGGGCA ACGTCAGCGA GGAGACCATC 
ATCAAGGAAG TCGTCTGCCA GTCCAGCCAT TTGCGATCCC GCAGCTCCAT GCTGTTAATT 
CACGGCAGTC AGGGAAATGT GATTGTCTGG CATGGATCGA AGAGTGCTCC TCATACGCGT 
AATGTGGCTG TAGGAGTTGC TCAGGATCTG ATCAAGACGG TGCCTAAAGA TCTCTTCAGC 
GCCGAAACTG CCAACCTAAC TGAGGTGGAG GAGGGTTCCG ACGACGATCA GTGCAAGAAG 
GCCCTGGGAT TGAAGGATGA AAGAAATGAA TATGGCAGCC TGCTTAAGTC CACCAAGTCG 
TTCGATTACC AAATAAGGAT CTTCAACTTC TCCAGCACTC AGGGTGTTTT CAAAGCCCTC 
GAACTGAGCG ATCCGCTGCG CTGCCAGGAT CTGCATAGTC CGTATCCCTT CAGCCAGTCG 
CAGCTGTACA ATGCAAGGCA GCCGACTATC TTTTTGCTGG ACGACGGCGA TGAGTTGTGG 
CTCTGGATGG GATGGTGGCC TCTGGAAGAT GTGAAGATAA ACACCGATGA GCGAAGCAGT 
CCCACCAACG AAAATCGCGC TGGAGTAAAT CGCTGGATCT CAGAGAGACG TGCTGCCCTC 
GAGACAGCCG TTGACTATTG GCGTGCCAAG CACGGCGAGA ACGAGAATGA ACCCTTCCAT 
GGCATCAAAG GCCAGGTTGT GTGGGCAGGC CTGGAGCCAC TGGCCTTTAA GGCGCTGTTT 
CCCGACTGGA CAGAACGTCC GGATGTAAGA GAAATCAACG AAGAAGATGG GCGAACTGAT 
GCTTCCATAT CGATCGCCAC AATGCTGGCA CAAATGACGC AGACCGAGTA TCCCCTGGAA 
GTCCTGAAAG CCCGACCGCT GCCCGAAGGT GTGGAGCCCA CCCGCCTGGA GGTCTACCTT 
AATGCGGAGG ACTTCCAGGC GGCACTGGGA TGCAGCCGGG CGGAATTCGA GCAGCTGCCC 
ATCTGGAAAC AGACCAAGCT GAAGAAGGAG CGAGGGCTGT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Svil ( NM_001202101.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001189030.1 Drosophila melanogaster supervillin, transcript variant M (Svil), mRNA.

Price & Availability↑

CloneID	OFa17295
Clone ID Related Accession (Same CDS sequence)	NM_001202101.1 , NM_001259637.1 , NM_206242.3 , NM_001274398.2
Accession Version	NM_001202101.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	supervillin, isoform M
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCAGCG GCAACTCGGG CGGCAGCAGT GGCAATCCCA AGCAGCAGCA GCAGCAGCAG 
CAGCAACAAC AGCTAGAGCA GCTGGGACAC CAGACACATC TTGTGCTAGG AGCATCAAAG 
GAGTTGCCCG CGGTCAGCAG CAGCAGCGAT GAAGAGGGAT CCCTGCGGAC TCTCCACTCC 
AGTCGCAAGG TGACCAATCT GAATGCCAAG TCCACCTCTC TGCCACCCGA GGAAGGACTC 
TCGGGTGATG TCTCCCTGGA GTCGTCCTCC AACCGACTGA ATCTGCGATT GGCCAGGAGC 
CTGGAGCCCT CAGTCCGCAA TATCACTGCA TCCGCCTCCA AGGAAGTACA GAGCTATGAT 
GCTGGGGAGC CTGTGAATAC CAATCGATTG TTTGTGGAAC CGCCCAAGAT ACCACTGTCT 
GCCATCCGAA ACTCTCTTGA CGATCACGAC CTGGATATTG AGGAGTGCGA CAGCACCACC 
CAGTTCGATC AGGGGGATAT CTACGATACC CTGGAACGCT GGAGCCATGG TGTCAGCTTG 
GGGGCACATC TATTGAACTG CGCGAATGGC CACGATCAAT CTGATCCCGA GGACGACCAT 
CAAACCGATC ATCATCAGCA TGCACATCAA TCATTGCCAG TAGAACCCAA GTCTCATCAT 
CATCAGCACC ACCAGGCCAC CAATGCGCGC AGATTCATAA CGCGCACCAC GCGTTCCGCC 
AGTCGCCAGA GTTCCGCCGG ACACTCCCCA CCTCCGCCGC TGCATCGCAG TGACGACTCC 
TCCAGCAGTT CACCCTCACC GACACGCAGA CGGAATAAGC CAGGTGGTGC TCCTGCCGCC 
ACAGATCCAC CATCATCGTC GGCGAACGGG AATAGTTCCG CTTATGAGAC GGCAGCCACC 
ACTGTGATGA CCCATGACCA GCAGCGAGAT CCTCCAGTTG AAGTTCCTAA GGCTCTAAAC 
GTCGAGCCAC CGCAGCCAGA AGACATCGTC CTGGGCTCCT GCCCCACTGC ATCTGCCCAC 
TCAGCAGAGA GCCAAAGTCG AGGAAAAGCT GGCAAATGCA GTATTAATTG CACCTACAGC 
CACAGCCACA GCACCAACAA CAACTGCAAC GCGCGACAAC GCAACAGCAG CAACATCAGC 
AGCAACAACA CCATCAGCAG CAACAGCAGC AGCAGCAAAA GCACTTGCAA CAACAATAGC 
AAACAGCAAC ACGCCACGCC CATGCCGCCC CTAGCTAATG CCAACAAAGC CCCGTTAATT 
GTGCTTAAGA CAGCCGCATC TGCAGCAGCA TTGCCGTCCA TTGCCGCAGC AGCAACATCC 
AGCGGCACGG CAGCAACATC TGGTGGTGTT GGTTCGCCCA TCAAGCGTCG CAAGAATCCG 
CTGTCGCCGC TGCTCCTCGG CAGCCAGTGC CAGTCCACCA TTGCGCCTCC GCAGGCCATT 
GCCAGTCCGC TCCTGAGCGG CGGCAGCTGC TCCTCCTCGA CCACCTCGCC CCAATCCTCG 
CCAGCGGTTC GCACCACCAA GGCGAGTCGT CTGCGCGCCG CCGCGCTTGA TAAAAAGAAG 
GAAGAGGAGC AACGCAATCG CTACAGTTCG CCCGTTTCAC CAAAAGCCTC CAGCAACGGC 
GGAGTCCAAG CACAACGTCG TCTTAGCAAC TCGGAGCTAT CGCCCAAGTC GCCAGGTGCC 
AGTGGATTCA CTTCGGAGCC AGTCGCGTCA GCCACAGCGA ACAGAGTCCC GACGAGATCT 
GCCTCCCATC CTGTGGACAC ACCGCCCACA CAAAGGCGAC AGCCTCTGGT GGCCGTGAGT 
TCGGGCATCT CCATGAAGCA GAGTCTCAGC GAGCTGCACT TCACGGGTGG AGCAGTCAGT 
CCGCCCAAGG CTACGGCGGA ATGCACTCTA AGGATGCGAC CACGTAGCTC CACGATGAGC 
ACCGAGAACA CGGAGAATGG CCGACGTGAC CACTTGGCTA ATCTGCAACT AAGTCCTGTG 
CAAAAGCAAA AGCGAACGCC CGAAGTATCA CCACGCCGTT TCGGAGATCG CGACAAGTCG 
GCCAAACGGA AATCGAATTT GAATCGTTCG TTGACCGAGG AAGCCACCAA GGATGGCGAT 
GAGGTTGCCT CTTCGACCCG CCGGCGCCGA CGTCGTGAAC TGGGAATGGC TCGTCCCCTG 
GTTCCGGGCG ATGATGACGC CCTAAGAGAT TTGCTAACGC CGCAGAAGAG TTCCTGCAGC 
GAGTCAACCA ACTCCATATC CGAACCCCAT CTCCAGTTCA TACCAGCCGT TCGTCATCCG 
GTGGTTCCAG TACGCACCAT GACTGCTGTG CTGCCTGCAC CACGACCGCT GCATACTCCT 
GCGGCCCGCA ACGTCAGCCA GAAGTTCAAT GAAAGGACCA AAACAGATCT GGCCGAGATC 
AAGAAGATTG CTGAGCAATC GGAGGCGCAG GTGCAACAGC CTTCGGTGGA TGATAACCAG 
AAAATGTCAG CCATAATGCG CAGGAAGAGC ACGGATGAGG GTGGATCAGC TTTGGGGGCA 
ACAAACCAGA ATTCACCACC TGCCCCAAAC CAAATTGTAT CCATACTGAA GAAGAAGGAA 
CATGGACTTG GCGAGTGCAA CTCCAGTGCT TCAAGCAATC CCTCGCCGGT GACCTTCTCT 
TCTAGCGTGG TGGATCATCC TTTGGCTGGA GCTGGCCGGT GCAAACGGCA GGGAATACTC 
AAGAAGAGAT CGAGTCTAGA TGAATCGCGC TACTACTCCC GATCGCATTC ACCCGACGAA 
CGTAGTATTC TCATTAAGTC GGCCAGGAGG AATTCCCTGG AGGAAGCTGG CACTGGATTG 
AGTCCGGCTC AGGCCCATGG AATCCTCAAG CAGAGCAGCT ACGACAGCAG CAAGAGCGAC 
GGCTGCCCTT CGGCCACGGA GAGCCAGCCA CACAGTATCC TGAAAAAGAA GGATTCGCTA 
TCGACACCTT CGGACGGAGG ATGCCACAAG CATGTCTCTA TATCGCAAGC TGTAACTTTA 
GCTGCTGCTG AACTGGCTGC CCATGATGGA ATCACCTTCG ATGATGGTGA GGAGCATGAG 
ATACGACCGA TCCTAAAGCA GGAGAGCACC TCCAGCGAGG AGGCTGTGCG TCCGCCAAAG 
CCCATACTCA AAAAGAAATC CTTTGGCGAG GCGGATGAAC ACGAAATACG TCCTATATTG 
AAGAGCTCTC GTAAAAGCAG TCGAGAGGAG TTCGATTTGA GTGGCTTGGA AAACGAGGTC 
AACGATTCCC TATCGTCAAT CCTAAAGACA GACTCGCCGT CCAAGCGTCG GTCGCTCGGT 
TCAGCTTTGC ATGATTTGGA GGAGTCCACC ACTTCCCCGA GCATGCTCAA GAGGCGCACC 
CGTTCGCTGG AACGTCAAGA TGTCCAGCCT GGCATGGATT TGGCAGCTGC ACTGGATGCC 
ATCGCCACAT CACAGGCTAC TCCCAGCACC CCGGTGGACT TCGTGACCAC ACCAACAAGT 
ATATCGGTGG CGGAGCGGAT CAAGCGCATG GAGATGCTGT CGACGCCCAC TTCCCGTGCG 
GCGGGTGGGG CCAACAGCAG CTGGGAATCG CCACTGCAGG CATCGTCAGC AGAGCATGGA 
GCACCCACCA CCCCAAGGGT TCTCAAGCCA AGTGTGCTGC GCAGAGATTT GCAGAGGGAG 
CGGTACAAAA CGCAACCCGT GACCAACGAG GAGATGAGCT TCTTCAAAGC CAACTCCGCG 
CCCTTCGACA TCTCGGCTAC ATCGGCCTTC AAGCCCACCA AGCCGCTGGA CATCCGCTTG 
ACCCACGCAC CGCATGCACC GCTGATTTCG CCGATCACCG GACAGCCACT CAGCCACGTG 
CCCGCGCCAC TGCTGCTATC CTCGTCGACG AACTCGGGAG CGGGATCCTC GTTCCGTCTG 
GCCCGCAGCT CCACCCAACC GCTGCAGTCG CCGCGAGTTG TCCACCTGGC CGGTCAGGCT 
ACTGGTGGCT CCGCTTTGGC ACGCCAGAAC TCGGTGAACG ACAGCATCAA TCTCAGCGAG 
CAGCTAACCC TATCCATCGG CACGGACAGC GAGCACATCT TTGAGATGTC CGCTTTGGAG 
GAGGACTCGG CTATTCAGTC GCTGAGCGAC GAGACAGCCG CTTCCGCATC TGCCAACTCA 
ACATCCACGA CCACTGCAAC CACCATGACA CCGCCCAGTG GCCTGACGCG GAGCAACAGC 
GTTCGGGCCA GGGCCAATAT GTTCCAGCAA TTGCAGGAGC AGTCGCGCAA CCAACGGGCA 
ACGGGAACCA CCAACTCCCG TCAATCTCCA CCAGCGTCGT CGGCGGAGGT GTCTTCAAGT 
GCAGCCGACA ATTCCATGCA CAGTGACGAG CGGTCCACGC CAAATGCAAC GATAGATGCG 
GAATTCGAAC CCTCATCCCT GTCGCTGGCT GAGCGACTGG CCTTCTTCAG CAATCTGTGC 
GAGAGCGGCG GAGGAGGTGG GAGTACCAGT GCCGGTGGAA GGTACCGCAG TCGCACCAGC 
AGCTATTCAA GGAGTCCGCC CGGCGATCGC ACCACTCCCA GGAGCAGCAC CACCGCCAGC 
AGTGTCAGTA TGACGCCCAC GCCCATGGAT TACTCTCCGA TTCCCCACGA CAAGGAGCCA 
TCTAGTCTCA CGCTGGAGAG CATCATCGAG CCCGAGCAGG AGGAGGTCAA GGAGCAGGTG 
GACCAGGATG AGGTCAAGTT GAGGAAGAAG GAGCCAGTCC AGAGGGAATC TTCCCAGCAC 
AATGGCTTCC ACGTGCTGAC CAGGTCCGCC ACCGAACCGC CGTCGTCCAC CTCGCCCCTG 
CGCATCAGCA TACGGACTGT GGGAAAGCTA GTCCTGCCCG ATACCTTCCG CGGCGATCGC 
AACAATAACA GCAGTAGTAG AAGTGGGAGC AACAGCTGGA GCAACACTTG CATGGTCAAG 
CTGCAGAGCT TGGAGCCCGT TAGCCTGTCC GTTCATTCGC GCACCATTGG TAAGGTTAAG 
TCGCCATTCA TTGAAAAGCA GCAGGAGAAA CCACACACCG AGAAGCTAGC GAACGGAACC 
AGCGACAGTG GCTCCGATTC GGGCAAGGAG AACTGCGCCT CCAACGAGCA GGAACATCTG 
CAGCACCAGC AGTCGCAGGA CGAGGATCGA GCTCCGCCCA GGGTGTCCGA GATGCGGAGG 
AAAATCAGCC GACTGGTGCA ACAACAGCAG CCGCAACCAC AGCCCGTCAA TCGGCGGCAC 
ACCACCGAGA TCACCTCCGT GACCGTTGGA ACGCGTTCGC CCAGTGTGGA CGATCGCTTT 
GCCAAGTACT TTGGTGTTAA GGAGAGCTGC AACAGCACGA CCACCGTGCA TAAAACGCAA 
TCGCTGCCAC CCAGCCAAGT TGAGGCCACC AATGCGCACG TGAAACTACC GCAGATCACT 
ACCGTGGTTT CCAAACGGCG CGAGCTGCTC AAGAGGACGC GACCCCTGTC CATGGATCAC 
TACTCCAGCG GCTGCACCAG TCCCACAGCC ATGCCAAGTC CCAAGAGAGC ACATTCGCCC 
GTCGGCCGGC GGAAGGCCGC CATTTCCAGC ATCGAGGACA TTGAGGTCAC GCCGGATGAA 
CTCCGGGCCG CTGGCAGGAA CTTCAAGCTG TTGTACGGCG AGTTATTGGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189030.1
CDS	1128..6761
Translation	MSSGNSGGSSGNPKQQQQQQQQQQLEQLGHQTHLVLGASKELPAVSSSSDEEGSLRTLHSSRKVTNLNAKSTSLPPEEGLSGDVSLESSSNRLNLRLARSLEPSVRNITASASKEVQSYDAGEPVNTNRLFVEPPKIPLSAIRNSLDDHDLDIEECDSTTQFDQGDIYDTLERWSHGVSLGAHLLNCANGHDQSDPEDDHQTDHHQHAHQSLPVEPKSHHHQHHQATNARRFITRTTRSASRQSSAGHSPPPPLHRSDDSSSSSPSPTRRRNKPGGAPAATDPPSSSANGNSSAYETAATTVMTHDQQRDPPVEVPKALNVEPPQPEDIVLGSCPTASAHSAESQSRGKAGKCSINCTYSHSHSTNNNCNARQRNSSNISSNNTISSNSSSSKSTCNNNSKQQHATPMPPLANANKAPLIVLKTAASAAALPSIAAAATSSGTAATSGGVGSPIKRRKNPLSPLLLGSQCQSTIAPPQAIASPLLSGGSCSSSTTSPQSSPAVRTTKASRLRAAALDKKKEEEQRNRYSSPVSPKASSNGGVQAQRRLSNSELSPKSPGASGFTSEPVASATANRVPTRSASHPVDTPPTQRRQPLVAVSSGISMKQSLSELHFTGGAVSPPKATAECTLRMRPRSSTMSTENTENGRRDHLANLQLSPVQKQKRTPEVSPRRFGDRDKSAKRKSNLNRSLTEEATKDGDEVASSTRRRRRRELGMARPLVPGDDDALRDLLTPQKSSCSESTNSISEPHLQFIPAVRHPVVPVRTMTAVLPAPRPLHTPAARNVSQKFNERTKTDLAEIKKIAEQSEAQVQQPSVDDNQKMSAIMRRKSTDEGGSALGATNQNSPPAPNQIVSILKKKEHGLGECNSSASSNPSPVTFSSSVVDHPLAGAGRCKRQGILKKRSSLDESRYYSRSHSPDERSILIKSARRNSLEEAGTGLSPAQAHGILKQSSYDSSKSDGCPSATESQPHSILKKKDSLSTPSDGGCHKHVSISQAVTLAAAELAAHDGITFDDGEEHEIRPILKQESTSSEEAVRPPKPILKKKSFGEADEHEIRPILKSSRKSSREEFDLSGLENEVNDSLSSILKTDSPSKRRSLGSALHDLEESTTSPSMLKRRTRSLERQDVQPGMDLAAALDAIATSQATPSTPVDFVTTPTSISVAERIKRMEMLSTPTSRAAGGANSSWESPLQASSAEHGAPTTPRVLKPSVLRRDLQRERYKTQPVTNEEMSFFKANSAPFDISATSAFKPTKPLDIRLTHAPHAPLISPITGQPLSHVPAPLLLSSSTNSGAGSSFRLARSSTQPLQSPRVVHLAGQATGGSALARQNSVNDSINLSEQLTLSIGTDSEHIFEMSALEEDSAIQSLSDETAASASANSTSTTTATTMTPPSGLTRSNSVRARANMFQQLQEQSRNQRATGTTNSRQSPPASSAEVSSSAADNSMHSDERSTPNATIDAEFEPSSLSLAERLAFFSNLCESGGGGGSTSAGGRYRSRTSSYSRSPPGDRTTPRSSTTASSVSMTPTPMDYSPIPHDKEPSSLTLESIIEPEQEEVKEQVDQDEVKLRKKEPVQRESSQHNGFHVLTRSATEPPSSTSPLRISIRTVGKLVLPDTFRGDRNNNSSSRSGSNSWSNTCMVKLQSLEPVSLSVHSRTIGKVKSPFIEKQQEKPHTEKLANGTSDSGSDSGKENCASNEQEHLQHQQSQDEDRAPPRVSEMRRKISRLVQQQQPQPQPVNRRHTTEITSVTVGTRSPSVDDRFAKYFGVKESCNSTTTVHKTQSLPPSQVEATNAHVKLPQITTVVSKRRELLKRTRPLSMDHYSSGCTSPTAMPSPKRAHSPVGRRKAAISSIEDIEVTPDELRAAGRNFKLLYGELLG

Target ORF information:

RefSeq Version	NM_001202101.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster supervillin, transcript variant M (Svil), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202101.1

ORF Insert Sequence:

ATGAGCAGCG GCAACTCGGG CGGCAGCAGT GGCAATCCCA AGCAGCAGCA GCAGCAGCAG 
CAGCAACAAC AGCTAGAGCA GCTGGGACAC CAGACACATC TTGTGCTAGG AGCATCAAAG 
GAGTTGCCCG CGGTCAGCAG CAGCAGCGAT GAAGAGGGAT CCCTGCGGAC TCTCCACTCC 
AGTCGCAAGG TGACCAATCT GAATGCCAAG TCCACCTCTC TGCCACCCGA GGAAGGACTC 
TCGGGTGATG TCTCCCTGGA GTCGTCCTCC AACCGACTGA ATCTGCGATT GGCCAGGAGC 
CTGGAGCCCT CAGTCCGCAA TATCACTGCA TCCGCCTCCA AGGAAGTACA GAGCTATGAT 
GCTGGGGAGC CTGTGAATAC CAATCGATTG TTTGTGGAAC CGCCCAAGAT ACCACTGTCT 
GCCATCCGAA ACTCTCTTGA CGATCACGAC CTGGATATTG AGGAGTGCGA CAGCACCACC 
CAGTTCGATC AGGGGGATAT CTACGATACC CTGGAACGCT GGAGCCATGG TGTCAGCTTG 
GGGGCACATC TATTGAACTG CGCGAATGGC CACGATCAAT CTGATCCCGA GGACGACCAT 
CAAACCGATC ATCATCAGCA TGCACATCAA TCATTGCCAG TAGAACCCAA GTCTCATCAT 
CATCAGCACC ACCAGGCCAC CAATGCGCGC AGATTCATAA CGCGCACCAC GCGTTCCGCC 
AGTCGCCAGA GTTCCGCCGG ACACTCCCCA CCTCCGCCGC TGCATCGCAG TGACGACTCC 
TCCAGCAGTT CACCCTCACC GACACGCAGA CGGAATAAGC CAGGTGGTGC TCCTGCCGCC 
ACAGATCCAC CATCATCGTC GGCGAACGGG AATAGTTCCG CTTATGAGAC GGCAGCCACC 
ACTGTGATGA CCCATGACCA GCAGCGAGAT CCTCCAGTTG AAGTTCCTAA GGCTCTAAAC 
GTCGAGCCAC CGCAGCCAGA AGACATCGTC CTGGGCTCCT GCCCCACTGC ATCTGCCCAC 
TCAGCAGAGA GCCAAAGTCG AGGAAAAGCT GGCAAATGCA GTATTAATTG CACCTACAGC 
CACAGCCACA GCACCAACAA CAACTGCAAC GCGCGACAAC GCAACAGCAG CAACATCAGC 
AGCAACAACA CCATCAGCAG CAACAGCAGC AGCAGCAAAA GCACTTGCAA CAACAATAGC 
AAACAGCAAC ACGCCACGCC CATGCCGCCC CTAGCTAATG CCAACAAAGC CCCGTTAATT 
GTGCTTAAGA CAGCCGCATC TGCAGCAGCA TTGCCGTCCA TTGCCGCAGC AGCAACATCC 
AGCGGCACGG CAGCAACATC TGGTGGTGTT GGTTCGCCCA TCAAGCGTCG CAAGAATCCG 
CTGTCGCCGC TGCTCCTCGG CAGCCAGTGC CAGTCCACCA TTGCGCCTCC GCAGGCCATT 
GCCAGTCCGC TCCTGAGCGG CGGCAGCTGC TCCTCCTCGA CCACCTCGCC CCAATCCTCG 
CCAGCGGTTC GCACCACCAA GGCGAGTCGT CTGCGCGCCG CCGCGCTTGA TAAAAAGAAG 
GAAGAGGAGC AACGCAATCG CTACAGTTCG CCCGTTTCAC CAAAAGCCTC CAGCAACGGC 
GGAGTCCAAG CACAACGTCG TCTTAGCAAC TCGGAGCTAT CGCCCAAGTC GCCAGGTGCC 
AGTGGATTCA CTTCGGAGCC AGTCGCGTCA GCCACAGCGA ACAGAGTCCC GACGAGATCT 
GCCTCCCATC CTGTGGACAC ACCGCCCACA CAAAGGCGAC AGCCTCTGGT GGCCGTGAGT 
TCGGGCATCT CCATGAAGCA GAGTCTCAGC GAGCTGCACT TCACGGGTGG AGCAGTCAGT 
CCGCCCAAGG CTACGGCGGA ATGCACTCTA AGGATGCGAC CACGTAGCTC CACGATGAGC 
ACCGAGAACA CGGAGAATGG CCGACGTGAC CACTTGGCTA ATCTGCAACT AAGTCCTGTG 
CAAAAGCAAA AGCGAACGCC CGAAGTATCA CCACGCCGTT TCGGAGATCG CGACAAGTCG 
GCCAAACGGA AATCGAATTT GAATCGTTCG TTGACCGAGG AAGCCACCAA GGATGGCGAT 
GAGGTTGCCT CTTCGACCCG CCGGCGCCGA CGTCGTGAAC TGGGAATGGC TCGTCCCCTG 
GTTCCGGGCG ATGATGACGC CCTAAGAGAT TTGCTAACGC CGCAGAAGAG TTCCTGCAGC 
GAGTCAACCA ACTCCATATC CGAACCCCAT CTCCAGTTCA TACCAGCCGT TCGTCATCCG 
GTGGTTCCAG TACGCACCAT GACTGCTGTG CTGCCTGCAC CACGACCGCT GCATACTCCT 
GCGGCCCGCA ACGTCAGCCA GAAGTTCAAT GAAAGGACCA AAACAGATCT GGCCGAGATC 
AAGAAGATTG CTGAGCAATC GGAGGCGCAG GTGCAACAGC CTTCGGTGGA TGATAACCAG 
AAAATGTCAG CCATAATGCG CAGGAAGAGC ACGGATGAGG GTGGATCAGC TTTGGGGGCA 
ACAAACCAGA ATTCACCACC TGCCCCAAAC CAAATTGTAT CCATACTGAA GAAGAAGGAA 
CATGGACTTG GCGAGTGCAA CTCCAGTGCT TCAAGCAATC CCTCGCCGGT GACCTTCTCT 
TCTAGCGTGG TGGATCATCC TTTGGCTGGA GCTGGCCGGT GCAAACGGCA GGGAATACTC 
AAGAAGAGAT CGAGTCTAGA TGAATCGCGC TACTACTCCC GATCGCATTC ACCCGACGAA 
CGTAGTATTC TCATTAAGTC GGCCAGGAGG AATTCCCTGG AGGAAGCTGG CACTGGATTG 
AGTCCGGCTC AGGCCCATGG AATCCTCAAG CAGAGCAGCT ACGACAGCAG CAAGAGCGAC 
GGCTGCCCTT CGGCCACGGA GAGCCAGCCA CACAGTATCC TGAAAAAGAA GGATTCGCTA 
TCGACACCTT CGGACGGAGG ATGCCACAAG CATGTCTCTA TATCGCAAGC TGTAACTTTA 
GCTGCTGCTG AACTGGCTGC CCATGATGGA ATCACCTTCG ATGATGGTGA GGAGCATGAG 
ATACGACCGA TCCTAAAGCA GGAGAGCACC TCCAGCGAGG AGGCTGTGCG TCCGCCAAAG 
CCCATACTCA AAAAGAAATC CTTTGGCGAG GCGGATGAAC ACGAAATACG TCCTATATTG 
AAGAGCTCTC GTAAAAGCAG TCGAGAGGAG TTCGATTTGA GTGGCTTGGA AAACGAGGTC 
AACGATTCCC TATCGTCAAT CCTAAAGACA GACTCGCCGT CCAAGCGTCG GTCGCTCGGT 
TCAGCTTTGC ATGATTTGGA GGAGTCCACC ACTTCCCCGA GCATGCTCAA GAGGCGCACC 
CGTTCGCTGG AACGTCAAGA TGTCCAGCCT GGCATGGATT TGGCAGCTGC ACTGGATGCC 
ATCGCCACAT CACAGGCTAC TCCCAGCACC CCGGTGGACT TCGTGACCAC ACCAACAAGT 
ATATCGGTGG CGGAGCGGAT CAAGCGCATG GAGATGCTGT CGACGCCCAC TTCCCGTGCG 
GCGGGTGGGG CCAACAGCAG CTGGGAATCG CCACTGCAGG CATCGTCAGC AGAGCATGGA 
GCACCCACCA CCCCAAGGGT TCTCAAGCCA AGTGTGCTGC GCAGAGATTT GCAGAGGGAG 
CGGTACAAAA CGCAACCCGT GACCAACGAG GAGATGAGCT TCTTCAAAGC CAACTCCGCG 
CCCTTCGACA TCTCGGCTAC ATCGGCCTTC AAGCCCACCA AGCCGCTGGA CATCCGCTTG 
ACCCACGCAC CGCATGCACC GCTGATTTCG CCGATCACCG GACAGCCACT CAGCCACGTG 
CCCGCGCCAC TGCTGCTATC CTCGTCGACG AACTCGGGAG CGGGATCCTC GTTCCGTCTG 
GCCCGCAGCT CCACCCAACC GCTGCAGTCG CCGCGAGTTG TCCACCTGGC CGGTCAGGCT 
ACTGGTGGCT CCGCTTTGGC ACGCCAGAAC TCGGTGAACG ACAGCATCAA TCTCAGCGAG 
CAGCTAACCC TATCCATCGG CACGGACAGC GAGCACATCT TTGAGATGTC CGCTTTGGAG 
GAGGACTCGG CTATTCAGTC GCTGAGCGAC GAGACAGCCG CTTCCGCATC TGCCAACTCA 
ACATCCACGA CCACTGCAAC CACCATGACA CCGCCCAGTG GCCTGACGCG GAGCAACAGC 
GTTCGGGCCA GGGCCAATAT GTTCCAGCAA TTGCAGGAGC AGTCGCGCAA CCAACGGGCA 
ACGGGAACCA CCAACTCCCG TCAATCTCCA CCAGCGTCGT CGGCGGAGGT GTCTTCAAGT 
GCAGCCGACA ATTCCATGCA CAGTGACGAG CGGTCCACGC CAAATGCAAC GATAGATGCG 
GAATTCGAAC CCTCATCCCT GTCGCTGGCT GAGCGACTGG CCTTCTTCAG CAATCTGTGC 
GAGAGCGGCG GAGGAGGTGG GAGTACCAGT GCCGGTGGAA GGTACCGCAG TCGCACCAGC 
AGCTATTCAA GGAGTCCGCC CGGCGATCGC ACCACTCCCA GGAGCAGCAC CACCGCCAGC 
AGTGTCAGTA TGACGCCCAC GCCCATGGAT TACTCTCCGA TTCCCCACGA CAAGGAGCCA 
TCTAGTCTCA CGCTGGAGAG CATCATCGAG CCCGAGCAGG AGGAGGTCAA GGAGCAGGTG 
GACCAGGATG AGGTCAAGTT GAGGAAGAAG GAGCCAGTCC AGAGGGAATC TTCCCAGCAC 
AATGGCTTCC ACGTGCTGAC CAGGTCCGCC ACCGAACCGC CGTCGTCCAC CTCGCCCCTG 
CGCATCAGCA TACGGACTGT GGGAAAGCTA GTCCTGCCCG ATACCTTCCG CGGCGATCGC 
AACAATAACA GCAGTAGTAG AAGTGGGAGC AACAGCTGGA GCAACACTTG CATGGTCAAG 
CTGCAGAGCT TGGAGCCCGT TAGCCTGTCC GTTCATTCGC GCACCATTGG TAAGGTTAAG 
TCGCCATTCA TTGAAAAGCA GCAGGAGAAA CCACACACCG AGAAGCTAGC GAACGGAACC 
AGCGACAGTG GCTCCGATTC GGGCAAGGAG AACTGCGCCT CCAACGAGCA GGAACATCTG 
CAGCACCAGC AGTCGCAGGA CGAGGATCGA GCTCCGCCCA GGGTGTCCGA GATGCGGAGG 
AAAATCAGCC GACTGGTGCA ACAACAGCAG CCGCAACCAC AGCCCGTCAA TCGGCGGCAC 
ACCACCGAGA TCACCTCCGT GACCGTTGGA ACGCGTTCGC CCAGTGTGGA CGATCGCTTT 
GCCAAGTACT TTGGTGTTAA GGAGAGCTGC AACAGCACGA CCACCGTGCA TAAAACGCAA 
TCGCTGCCAC CCAGCCAAGT TGAGGCCACC AATGCGCACG TGAAACTACC GCAGATCACT 
ACCGTGGTTT CCAAACGGCG CGAGCTGCTC AAGAGGACGC GACCCCTGTC CATGGATCAC 
TACTCCAGCG GCTGCACCAG TCCCACAGCC ATGCCAAGTC CCAAGAGAGC ACATTCGCCC 
GTCGGCCGGC GGAAGGCCGC CATTTCCAGC ATCGAGGACA TTGAGGTCAC GCCGGATGAA 
CTCCGGGCCG CTGGCAGGAA CTTCAAGCTG TTGTACGGCG AGTTATTGGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Svil ( NM_001259637.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246566.1 Drosophila melanogaster supervillin, transcript variant Q (Svil), mRNA.

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CloneID	OFa17295
Clone ID Related Accession (Same CDS sequence)	NM_001202101.1 , NM_001259637.1 , NM_206242.3 , NM_001274398.2
Accession Version	NM_001259637.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	supervillin, isoform Q
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCAGCG GCAACTCGGG CGGCAGCAGT GGCAATCCCA AGCAGCAGCA GCAGCAGCAG 
CAGCAACAAC AGCTAGAGCA GCTGGGACAC CAGACACATC TTGTGCTAGG AGCATCAAAG 
GAGTTGCCCG CGGTCAGCAG CAGCAGCGAT GAAGAGGGAT CCCTGCGGAC TCTCCACTCC 
AGTCGCAAGG TGACCAATCT GAATGCCAAG TCCACCTCTC TGCCACCCGA GGAAGGACTC 
TCGGGTGATG TCTCCCTGGA GTCGTCCTCC AACCGACTGA ATCTGCGATT GGCCAGGAGC 
CTGGAGCCCT CAGTCCGCAA TATCACTGCA TCCGCCTCCA AGGAAGTACA GAGCTATGAT 
GCTGGGGAGC CTGTGAATAC CAATCGATTG TTTGTGGAAC CGCCCAAGAT ACCACTGTCT 
GCCATCCGAA ACTCTCTTGA CGATCACGAC CTGGATATTG AGGAGTGCGA CAGCACCACC 
CAGTTCGATC AGGGGGATAT CTACGATACC CTGGAACGCT GGAGCCATGG TGTCAGCTTG 
GGGGCACATC TATTGAACTG CGCGAATGGC CACGATCAAT CTGATCCCGA GGACGACCAT 
CAAACCGATC ATCATCAGCA TGCACATCAA TCATTGCCAG TAGAACCCAA GTCTCATCAT 
CATCAGCACC ACCAGGCCAC CAATGCGCGC AGATTCATAA CGCGCACCAC GCGTTCCGCC 
AGTCGCCAGA GTTCCGCCGG ACACTCCCCA CCTCCGCCGC TGCATCGCAG TGACGACTCC 
TCCAGCAGTT CACCCTCACC GACACGCAGA CGGAATAAGC CAGGTGGTGC TCCTGCCGCC 
ACAGATCCAC CATCATCGTC GGCGAACGGG AATAGTTCCG CTTATGAGAC GGCAGCCACC 
ACTGTGATGA CCCATGACCA GCAGCGAGAT CCTCCAGTTG AAGTTCCTAA GGCTCTAAAC 
GTCGAGCCAC CGCAGCCAGA AGACATCGTC CTGGGCTCCT GCCCCACTGC ATCTGCCCAC 
TCAGCAGAGA GCCAAAGTCG AGGAAAAGCT GGCAAATGCA GTATTAATTG CACCTACAGC 
CACAGCCACA GCACCAACAA CAACTGCAAC GCGCGACAAC GCAACAGCAG CAACATCAGC 
AGCAACAACA CCATCAGCAG CAACAGCAGC AGCAGCAAAA GCACTTGCAA CAACAATAGC 
AAACAGCAAC ACGCCACGCC CATGCCGCCC CTAGCTAATG CCAACAAAGC CCCGTTAATT 
GTGCTTAAGA CAGCCGCATC TGCAGCAGCA TTGCCGTCCA TTGCCGCAGC AGCAACATCC 
AGCGGCACGG CAGCAACATC TGGTGGTGTT GGTTCGCCCA TCAAGCGTCG CAAGAATCCG 
CTGTCGCCGC TGCTCCTCGG CAGCCAGTGC CAGTCCACCA TTGCGCCTCC GCAGGCCATT 
GCCAGTCCGC TCCTGAGCGG CGGCAGCTGC TCCTCCTCGA CCACCTCGCC CCAATCCTCG 
CCAGCGGTTC GCACCACCAA GGCGAGTCGT CTGCGCGCCG CCGCGCTTGA TAAAAAGAAG 
GAAGAGGAGC AACGCAATCG CTACAGTTCG CCCGTTTCAC CAAAAGCCTC CAGCAACGGC 
GGAGTCCAAG CACAACGTCG TCTTAGCAAC TCGGAGCTAT CGCCCAAGTC GCCAGGTGCC 
AGTGGATTCA CTTCGGAGCC AGTCGCGTCA GCCACAGCGA ACAGAGTCCC GACGAGATCT 
GCCTCCCATC CTGTGGACAC ACCGCCCACA CAAAGGCGAC AGCCTCTGGT GGCCGTGAGT 
TCGGGCATCT CCATGAAGCA GAGTCTCAGC GAGCTGCACT TCACGGGTGG AGCAGTCAGT 
CCGCCCAAGG CTACGGCGGA ATGCACTCTA AGGATGCGAC CACGTAGCTC CACGATGAGC 
ACCGAGAACA CGGAGAATGG CCGACGTGAC CACTTGGCTA ATCTGCAACT AAGTCCTGTG 
CAAAAGCAAA AGCGAACGCC CGAAGTATCA CCACGCCGTT TCGGAGATCG CGACAAGTCG 
GCCAAACGGA AATCGAATTT GAATCGTTCG TTGACCGAGG AAGCCACCAA GGATGGCGAT 
GAGGTTGCCT CTTCGACCCG CCGGCGCCGA CGTCGTGAAC TGGGAATGGC TCGTCCCCTG 
GTTCCGGGCG ATGATGACGC CCTAAGAGAT TTGCTAACGC CGCAGAAGAG TTCCTGCAGC 
GAGTCAACCA ACTCCATATC CGAACCCCAT CTCCAGTTCA TACCAGCCGT TCGTCATCCG 
GTGGTTCCAG TACGCACCAT GACTGCTGTG CTGCCTGCAC CACGACCGCT GCATACTCCT 
GCGGCCCGCA ACGTCAGCCA GAAGTTCAAT GAAAGGACCA AAACAGATCT GGCCGAGATC 
AAGAAGATTG CTGAGCAATC GGAGGCGCAG GTGCAACAGC CTTCGGTGGA TGATAACCAG 
AAAATGTCAG CCATAATGCG CAGGAAGAGC ACGGATGAGG GTGGATCAGC TTTGGGGGCA 
ACAAACCAGA ATTCACCACC TGCCCCAAAC CAAATTGTAT CCATACTGAA GAAGAAGGAA 
CATGGACTTG GCGAGTGCAA CTCCAGTGCT TCAAGCAATC CCTCGCCGGT GACCTTCTCT 
TCTAGCGTGG TGGATCATCC TTTGGCTGGA GCTGGCCGGT GCAAACGGCA GGGAATACTC 
AAGAAGAGAT CGAGTCTAGA TGAATCGCGC TACTACTCCC GATCGCATTC ACCCGACGAA 
CGTAGTATTC TCATTAAGTC GGCCAGGAGG AATTCCCTGG AGGAAGCTGG CACTGGATTG 
AGTCCGGCTC AGGCCCATGG AATCCTCAAG CAGAGCAGCT ACGACAGCAG CAAGAGCGAC 
GGCTGCCCTT CGGCCACGGA GAGCCAGCCA CACAGTATCC TGAAAAAGAA GGATTCGCTA 
TCGACACCTT CGGACGGAGG ATGCCACAAG CATGTCTCTA TATCGCAAGC TGTAACTTTA 
GCTGCTGCTG AACTGGCTGC CCATGATGGA ATCACCTTCG ATGATGGTGA GGAGCATGAG 
ATACGACCGA TCCTAAAGCA GGAGAGCACC TCCAGCGAGG AGGCTGTGCG TCCGCCAAAG 
CCCATACTCA AAAAGAAATC CTTTGGCGAG GCGGATGAAC ACGAAATACG TCCTATATTG 
AAGAGCTCTC GTAAAAGCAG TCGAGAGGAG TTCGATTTGA GTGGCTTGGA AAACGAGGTC 
AACGATTCCC TATCGTCAAT CCTAAAGACA GACTCGCCGT CCAAGCGTCG GTCGCTCGGT 
TCAGCTTTGC ATGATTTGGA GGAGTCCACC ACTTCCCCGA GCATGCTCAA GAGGCGCACC 
CGTTCGCTGG AACGTCAAGA TGTCCAGCCT GGCATGGATT TGGCAGCTGC ACTGGATGCC 
ATCGCCACAT CACAGGCTAC TCCCAGCACC CCGGTGGACT TCGTGACCAC ACCAACAAGT 
ATATCGGTGG CGGAGCGGAT CAAGCGCATG GAGATGCTGT CGACGCCCAC TTCCCGTGCG 
GCGGGTGGGG CCAACAGCAG CTGGGAATCG CCACTGCAGG CATCGTCAGC AGAGCATGGA 
GCACCCACCA CCCCAAGGGT TCTCAAGCCA AGTGTGCTGC GCAGAGATTT GCAGAGGGAG 
CGGTACAAAA CGCAACCCGT GACCAACGAG GAGATGAGCT TCTTCAAAGC CAACTCCGCG 
CCCTTCGACA TCTCGGCTAC ATCGGCCTTC AAGCCCACCA AGCCGCTGGA CATCCGCTTG 
ACCCACGCAC CGCATGCACC GCTGATTTCG CCGATCACCG GACAGCCACT CAGCCACGTG 
CCCGCGCCAC TGCTGCTATC CTCGTCGACG AACTCGGGAG CGGGATCCTC GTTCCGTCTG 
GCCCGCAGCT CCACCCAACC GCTGCAGTCG CCGCGAGTTG TCCACCTGGC CGGTCAGGCT 
ACTGGTGGCT CCGCTTTGGC ACGCCAGAAC TCGGTGAACG ACAGCATCAA TCTCAGCGAG 
CAGCTAACCC TATCCATCGG CACGGACAGC GAGCACATCT TTGAGATGTC CGCTTTGGAG 
GAGGACTCGG CTATTCAGTC GCTGAGCGAC GAGACAGCCG CTTCCGCATC TGCCAACTCA 
ACATCCACGA CCACTGCAAC CACCATGACA CCGCCCAGTG GCCTGACGCG GAGCAACAGC 
GTTCGGGCCA GGGCCAATAT GTTCCAGCAA TTGCAGGAGC AGTCGCGCAA CCAACGGGCA 
ACGGGAACCA CCAACTCCCG TCAATCTCCA CCAGCGTCGT CGGCGGAGGT GTCTTCAAGT 
GCAGCCGACA ATTCCATGCA CAGTGACGAG CGGTCCACGC CAAATGCAAC GATAGATGCG 
GAATTCGAAC CCTCATCCCT GTCGCTGGCT GAGCGACTGG CCTTCTTCAG CAATCTGTGC 
GAGAGCGGCG GAGGAGGTGG GAGTACCAGT GCCGGTGGAA GGTACCGCAG TCGCACCAGC 
AGCTATTCAA GGAGTCCGCC CGGCGATCGC ACCACTCCCA GGAGCAGCAC CACCGCCAGC 
AGTGTCAGTA TGACGCCCAC GCCCATGGAT TACTCTCCGA TTCCCCACGA CAAGGAGCCA 
TCTAGTCTCA CGCTGGAGAG CATCATCGAG CCCGAGCAGG AGGAGGTCAA GGAGCAGGTG 
GACCAGGATG AGGTCAAGTT GAGGAAGAAG GAGCCAGTCC AGAGGGAATC TTCCCAGCAC 
AATGGCTTCC ACGTGCTGAC CAGGTCCGCC ACCGAACCGC CGTCGTCCAC CTCGCCCCTG 
CGCATCAGCA TACGGACTGT GGGAAAGCTA GTCCTGCCCG ATACCTTCCG CGGCGATCGC 
AACAATAACA GCAGTAGTAG AAGTGGGAGC AACAGCTGGA GCAACACTTG CATGGTCAAG 
CTGCAGAGCT TGGAGCCCGT TAGCCTGTCC GTTCATTCGC GCACCATTGG TAAGGTTAAG 
TCGCCATTCA TTGAAAAGCA GCAGGAGAAA CCACACACCG AGAAGCTAGC GAACGGAACC 
AGCGACAGTG GCTCCGATTC GGGCAAGGAG AACTGCGCCT CCAACGAGCA GGAACATCTG 
CAGCACCAGC AGTCGCAGGA CGAGGATCGA GCTCCGCCCA GGGTGTCCGA GATGCGGAGG 
AAAATCAGCC GACTGGTGCA ACAACAGCAG CCGCAACCAC AGCCCGTCAA TCGGCGGCAC 
ACCACCGAGA TCACCTCCGT GACCGTTGGA ACGCGTTCGC CCAGTGTGGA CGATCGCTTT 
GCCAAGTACT TTGGTGTTAA GGAGAGCTGC AACAGCACGA CCACCGTGCA TAAAACGCAA 
TCGCTGCCAC CCAGCCAAGT TGAGGCCACC AATGCGCACG TGAAACTACC GCAGATCACT 
ACCGTGGTTT CCAAACGGCG CGAGCTGCTC AAGAGGACGC GACCCCTGTC CATGGATCAC 
TACTCCAGCG GCTGCACCAG TCCCACAGCC ATGCCAAGTC CCAAGAGAGC ACATTCGCCC 
GTCGGCCGGC GGAAGGCCGC CATTTCCAGC ATCGAGGACA TTGAGGTCAC GCCGGATGAA 
CTCCGGGCCG CTGGCAGGAA CTTCAAGCTG TTGTACGGCG AGTTATTGGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246566.1
CDS	1293..6926
Translation	MSSGNSGGSSGNPKQQQQQQQQQQLEQLGHQTHLVLGASKELPAVSSSSDEEGSLRTLHSSRKVTNLNAKSTSLPPEEGLSGDVSLESSSNRLNLRLARSLEPSVRNITASASKEVQSYDAGEPVNTNRLFVEPPKIPLSAIRNSLDDHDLDIEECDSTTQFDQGDIYDTLERWSHGVSLGAHLLNCANGHDQSDPEDDHQTDHHQHAHQSLPVEPKSHHHQHHQATNARRFITRTTRSASRQSSAGHSPPPPLHRSDDSSSSSPSPTRRRNKPGGAPAATDPPSSSANGNSSAYETAATTVMTHDQQRDPPVEVPKALNVEPPQPEDIVLGSCPTASAHSAESQSRGKAGKCSINCTYSHSHSTNNNCNARQRNSSNISSNNTISSNSSSSKSTCNNNSKQQHATPMPPLANANKAPLIVLKTAASAAALPSIAAAATSSGTAATSGGVGSPIKRRKNPLSPLLLGSQCQSTIAPPQAIASPLLSGGSCSSSTTSPQSSPAVRTTKASRLRAAALDKKKEEEQRNRYSSPVSPKASSNGGVQAQRRLSNSELSPKSPGASGFTSEPVASATANRVPTRSASHPVDTPPTQRRQPLVAVSSGISMKQSLSELHFTGGAVSPPKATAECTLRMRPRSSTMSTENTENGRRDHLANLQLSPVQKQKRTPEVSPRRFGDRDKSAKRKSNLNRSLTEEATKDGDEVASSTRRRRRRELGMARPLVPGDDDALRDLLTPQKSSCSESTNSISEPHLQFIPAVRHPVVPVRTMTAVLPAPRPLHTPAARNVSQKFNERTKTDLAEIKKIAEQSEAQVQQPSVDDNQKMSAIMRRKSTDEGGSALGATNQNSPPAPNQIVSILKKKEHGLGECNSSASSNPSPVTFSSSVVDHPLAGAGRCKRQGILKKRSSLDESRYYSRSHSPDERSILIKSARRNSLEEAGTGLSPAQAHGILKQSSYDSSKSDGCPSATESQPHSILKKKDSLSTPSDGGCHKHVSISQAVTLAAAELAAHDGITFDDGEEHEIRPILKQESTSSEEAVRPPKPILKKKSFGEADEHEIRPILKSSRKSSREEFDLSGLENEVNDSLSSILKTDSPSKRRSLGSALHDLEESTTSPSMLKRRTRSLERQDVQPGMDLAAALDAIATSQATPSTPVDFVTTPTSISVAERIKRMEMLSTPTSRAAGGANSSWESPLQASSAEHGAPTTPRVLKPSVLRRDLQRERYKTQPVTNEEMSFFKANSAPFDISATSAFKPTKPLDIRLTHAPHAPLISPITGQPLSHVPAPLLLSSSTNSGAGSSFRLARSSTQPLQSPRVVHLAGQATGGSALARQNSVNDSINLSEQLTLSIGTDSEHIFEMSALEEDSAIQSLSDETAASASANSTSTTTATTMTPPSGLTRSNSVRARANMFQQLQEQSRNQRATGTTNSRQSPPASSAEVSSSAADNSMHSDERSTPNATIDAEFEPSSLSLAERLAFFSNLCESGGGGGSTSAGGRYRSRTSSYSRSPPGDRTTPRSSTTASSVSMTPTPMDYSPIPHDKEPSSLTLESIIEPEQEEVKEQVDQDEVKLRKKEPVQRESSQHNGFHVLTRSATEPPSSTSPLRISIRTVGKLVLPDTFRGDRNNNSSSRSGSNSWSNTCMVKLQSLEPVSLSVHSRTIGKVKSPFIEKQQEKPHTEKLANGTSDSGSDSGKENCASNEQEHLQHQQSQDEDRAPPRVSEMRRKISRLVQQQQPQPQPVNRRHTTEITSVTVGTRSPSVDDRFAKYFGVKESCNSTTTVHKTQSLPPSQVEATNAHVKLPQITTVVSKRRELLKRTRPLSMDHYSSGCTSPTAMPSPKRAHSPVGRRKAAISSIEDIEVTPDELRAAGRNFKLLYGELLG

Target ORF information:

RefSeq Version	NM_001259637.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster supervillin, transcript variant Q (Svil), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259637.1

ORF Insert Sequence:

ATGAGCAGCG GCAACTCGGG CGGCAGCAGT GGCAATCCCA AGCAGCAGCA GCAGCAGCAG 
CAGCAACAAC AGCTAGAGCA GCTGGGACAC CAGACACATC TTGTGCTAGG AGCATCAAAG 
GAGTTGCCCG CGGTCAGCAG CAGCAGCGAT GAAGAGGGAT CCCTGCGGAC TCTCCACTCC 
AGTCGCAAGG TGACCAATCT GAATGCCAAG TCCACCTCTC TGCCACCCGA GGAAGGACTC 
TCGGGTGATG TCTCCCTGGA GTCGTCCTCC AACCGACTGA ATCTGCGATT GGCCAGGAGC 
CTGGAGCCCT CAGTCCGCAA TATCACTGCA TCCGCCTCCA AGGAAGTACA GAGCTATGAT 
GCTGGGGAGC CTGTGAATAC CAATCGATTG TTTGTGGAAC CGCCCAAGAT ACCACTGTCT 
GCCATCCGAA ACTCTCTTGA CGATCACGAC CTGGATATTG AGGAGTGCGA CAGCACCACC 
CAGTTCGATC AGGGGGATAT CTACGATACC CTGGAACGCT GGAGCCATGG TGTCAGCTTG 
GGGGCACATC TATTGAACTG CGCGAATGGC CACGATCAAT CTGATCCCGA GGACGACCAT 
CAAACCGATC ATCATCAGCA TGCACATCAA TCATTGCCAG TAGAACCCAA GTCTCATCAT 
CATCAGCACC ACCAGGCCAC CAATGCGCGC AGATTCATAA CGCGCACCAC GCGTTCCGCC 
AGTCGCCAGA GTTCCGCCGG ACACTCCCCA CCTCCGCCGC TGCATCGCAG TGACGACTCC 
TCCAGCAGTT CACCCTCACC GACACGCAGA CGGAATAAGC CAGGTGGTGC TCCTGCCGCC 
ACAGATCCAC CATCATCGTC GGCGAACGGG AATAGTTCCG CTTATGAGAC GGCAGCCACC 
ACTGTGATGA CCCATGACCA GCAGCGAGAT CCTCCAGTTG AAGTTCCTAA GGCTCTAAAC 
GTCGAGCCAC CGCAGCCAGA AGACATCGTC CTGGGCTCCT GCCCCACTGC ATCTGCCCAC 
TCAGCAGAGA GCCAAAGTCG AGGAAAAGCT GGCAAATGCA GTATTAATTG CACCTACAGC 
CACAGCCACA GCACCAACAA CAACTGCAAC GCGCGACAAC GCAACAGCAG CAACATCAGC 
AGCAACAACA CCATCAGCAG CAACAGCAGC AGCAGCAAAA GCACTTGCAA CAACAATAGC 
AAACAGCAAC ACGCCACGCC CATGCCGCCC CTAGCTAATG CCAACAAAGC CCCGTTAATT 
GTGCTTAAGA CAGCCGCATC TGCAGCAGCA TTGCCGTCCA TTGCCGCAGC AGCAACATCC 
AGCGGCACGG CAGCAACATC TGGTGGTGTT GGTTCGCCCA TCAAGCGTCG CAAGAATCCG 
CTGTCGCCGC TGCTCCTCGG CAGCCAGTGC CAGTCCACCA TTGCGCCTCC GCAGGCCATT 
GCCAGTCCGC TCCTGAGCGG CGGCAGCTGC TCCTCCTCGA CCACCTCGCC CCAATCCTCG 
CCAGCGGTTC GCACCACCAA GGCGAGTCGT CTGCGCGCCG CCGCGCTTGA TAAAAAGAAG 
GAAGAGGAGC AACGCAATCG CTACAGTTCG CCCGTTTCAC CAAAAGCCTC CAGCAACGGC 
GGAGTCCAAG CACAACGTCG TCTTAGCAAC TCGGAGCTAT CGCCCAAGTC GCCAGGTGCC 
AGTGGATTCA CTTCGGAGCC AGTCGCGTCA GCCACAGCGA ACAGAGTCCC GACGAGATCT 
GCCTCCCATC CTGTGGACAC ACCGCCCACA CAAAGGCGAC AGCCTCTGGT GGCCGTGAGT 
TCGGGCATCT CCATGAAGCA GAGTCTCAGC GAGCTGCACT TCACGGGTGG AGCAGTCAGT 
CCGCCCAAGG CTACGGCGGA ATGCACTCTA AGGATGCGAC CACGTAGCTC CACGATGAGC 
ACCGAGAACA CGGAGAATGG CCGACGTGAC CACTTGGCTA ATCTGCAACT AAGTCCTGTG 
CAAAAGCAAA AGCGAACGCC CGAAGTATCA CCACGCCGTT TCGGAGATCG CGACAAGTCG 
GCCAAACGGA AATCGAATTT GAATCGTTCG TTGACCGAGG AAGCCACCAA GGATGGCGAT 
GAGGTTGCCT CTTCGACCCG CCGGCGCCGA CGTCGTGAAC TGGGAATGGC TCGTCCCCTG 
GTTCCGGGCG ATGATGACGC CCTAAGAGAT TTGCTAACGC CGCAGAAGAG TTCCTGCAGC 
GAGTCAACCA ACTCCATATC CGAACCCCAT CTCCAGTTCA TACCAGCCGT TCGTCATCCG 
GTGGTTCCAG TACGCACCAT GACTGCTGTG CTGCCTGCAC CACGACCGCT GCATACTCCT 
GCGGCCCGCA ACGTCAGCCA GAAGTTCAAT GAAAGGACCA AAACAGATCT GGCCGAGATC 
AAGAAGATTG CTGAGCAATC GGAGGCGCAG GTGCAACAGC CTTCGGTGGA TGATAACCAG 
AAAATGTCAG CCATAATGCG CAGGAAGAGC ACGGATGAGG GTGGATCAGC TTTGGGGGCA 
ACAAACCAGA ATTCACCACC TGCCCCAAAC CAAATTGTAT CCATACTGAA GAAGAAGGAA 
CATGGACTTG GCGAGTGCAA CTCCAGTGCT TCAAGCAATC CCTCGCCGGT GACCTTCTCT 
TCTAGCGTGG TGGATCATCC TTTGGCTGGA GCTGGCCGGT GCAAACGGCA GGGAATACTC 
AAGAAGAGAT CGAGTCTAGA TGAATCGCGC TACTACTCCC GATCGCATTC ACCCGACGAA 
CGTAGTATTC TCATTAAGTC GGCCAGGAGG AATTCCCTGG AGGAAGCTGG CACTGGATTG 
AGTCCGGCTC AGGCCCATGG AATCCTCAAG CAGAGCAGCT ACGACAGCAG CAAGAGCGAC 
GGCTGCCCTT CGGCCACGGA GAGCCAGCCA CACAGTATCC TGAAAAAGAA GGATTCGCTA 
TCGACACCTT CGGACGGAGG ATGCCACAAG CATGTCTCTA TATCGCAAGC TGTAACTTTA 
GCTGCTGCTG AACTGGCTGC CCATGATGGA ATCACCTTCG ATGATGGTGA GGAGCATGAG 
ATACGACCGA TCCTAAAGCA GGAGAGCACC TCCAGCGAGG AGGCTGTGCG TCCGCCAAAG 
CCCATACTCA AAAAGAAATC CTTTGGCGAG GCGGATGAAC ACGAAATACG TCCTATATTG 
AAGAGCTCTC GTAAAAGCAG TCGAGAGGAG TTCGATTTGA GTGGCTTGGA AAACGAGGTC 
AACGATTCCC TATCGTCAAT CCTAAAGACA GACTCGCCGT CCAAGCGTCG GTCGCTCGGT 
TCAGCTTTGC ATGATTTGGA GGAGTCCACC ACTTCCCCGA GCATGCTCAA GAGGCGCACC 
CGTTCGCTGG AACGTCAAGA TGTCCAGCCT GGCATGGATT TGGCAGCTGC ACTGGATGCC 
ATCGCCACAT CACAGGCTAC TCCCAGCACC CCGGTGGACT TCGTGACCAC ACCAACAAGT 
ATATCGGTGG CGGAGCGGAT CAAGCGCATG GAGATGCTGT CGACGCCCAC TTCCCGTGCG 
GCGGGTGGGG CCAACAGCAG CTGGGAATCG CCACTGCAGG CATCGTCAGC AGAGCATGGA 
GCACCCACCA CCCCAAGGGT TCTCAAGCCA AGTGTGCTGC GCAGAGATTT GCAGAGGGAG 
CGGTACAAAA CGCAACCCGT GACCAACGAG GAGATGAGCT TCTTCAAAGC CAACTCCGCG 
CCCTTCGACA TCTCGGCTAC ATCGGCCTTC AAGCCCACCA AGCCGCTGGA CATCCGCTTG 
ACCCACGCAC CGCATGCACC GCTGATTTCG CCGATCACCG GACAGCCACT CAGCCACGTG 
CCCGCGCCAC TGCTGCTATC CTCGTCGACG AACTCGGGAG CGGGATCCTC GTTCCGTCTG 
GCCCGCAGCT CCACCCAACC GCTGCAGTCG CCGCGAGTTG TCCACCTGGC CGGTCAGGCT 
ACTGGTGGCT CCGCTTTGGC ACGCCAGAAC TCGGTGAACG ACAGCATCAA TCTCAGCGAG 
CAGCTAACCC TATCCATCGG CACGGACAGC GAGCACATCT TTGAGATGTC CGCTTTGGAG 
GAGGACTCGG CTATTCAGTC GCTGAGCGAC GAGACAGCCG CTTCCGCATC TGCCAACTCA 
ACATCCACGA CCACTGCAAC CACCATGACA CCGCCCAGTG GCCTGACGCG GAGCAACAGC 
GTTCGGGCCA GGGCCAATAT GTTCCAGCAA TTGCAGGAGC AGTCGCGCAA CCAACGGGCA 
ACGGGAACCA CCAACTCCCG TCAATCTCCA CCAGCGTCGT CGGCGGAGGT GTCTTCAAGT 
GCAGCCGACA ATTCCATGCA CAGTGACGAG CGGTCCACGC CAAATGCAAC GATAGATGCG 
GAATTCGAAC CCTCATCCCT GTCGCTGGCT GAGCGACTGG CCTTCTTCAG CAATCTGTGC 
GAGAGCGGCG GAGGAGGTGG GAGTACCAGT GCCGGTGGAA GGTACCGCAG TCGCACCAGC 
AGCTATTCAA GGAGTCCGCC CGGCGATCGC ACCACTCCCA GGAGCAGCAC CACCGCCAGC 
AGTGTCAGTA TGACGCCCAC GCCCATGGAT TACTCTCCGA TTCCCCACGA CAAGGAGCCA 
TCTAGTCTCA CGCTGGAGAG CATCATCGAG CCCGAGCAGG AGGAGGTCAA GGAGCAGGTG 
GACCAGGATG AGGTCAAGTT GAGGAAGAAG GAGCCAGTCC AGAGGGAATC TTCCCAGCAC 
AATGGCTTCC ACGTGCTGAC CAGGTCCGCC ACCGAACCGC CGTCGTCCAC CTCGCCCCTG 
CGCATCAGCA TACGGACTGT GGGAAAGCTA GTCCTGCCCG ATACCTTCCG CGGCGATCGC 
AACAATAACA GCAGTAGTAG AAGTGGGAGC AACAGCTGGA GCAACACTTG CATGGTCAAG 
CTGCAGAGCT TGGAGCCCGT TAGCCTGTCC GTTCATTCGC GCACCATTGG TAAGGTTAAG 
TCGCCATTCA TTGAAAAGCA GCAGGAGAAA CCACACACCG AGAAGCTAGC GAACGGAACC 
AGCGACAGTG GCTCCGATTC GGGCAAGGAG AACTGCGCCT CCAACGAGCA GGAACATCTG 
CAGCACCAGC AGTCGCAGGA CGAGGATCGA GCTCCGCCCA GGGTGTCCGA GATGCGGAGG 
AAAATCAGCC GACTGGTGCA ACAACAGCAG CCGCAACCAC AGCCCGTCAA TCGGCGGCAC 
ACCACCGAGA TCACCTCCGT GACCGTTGGA ACGCGTTCGC CCAGTGTGGA CGATCGCTTT 
GCCAAGTACT TTGGTGTTAA GGAGAGCTGC AACAGCACGA CCACCGTGCA TAAAACGCAA 
TCGCTGCCAC CCAGCCAAGT TGAGGCCACC AATGCGCACG TGAAACTACC GCAGATCACT 
ACCGTGGTTT CCAAACGGCG CGAGCTGCTC AAGAGGACGC GACCCCTGTC CATGGATCAC 
TACTCCAGCG GCTGCACCAG TCCCACAGCC ATGCCAAGTC CCAAGAGAGC ACATTCGCCC 
GTCGGCCGGC GGAAGGCCGC CATTTCCAGC ATCGAGGACA TTGAGGTCAC GCCGGATGAA 
CTCCGGGCCG CTGGCAGGAA CTTCAAGCTG TTGTACGGCG AGTTATTGGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Svil ( NM_206242.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995964.2 Drosophila melanogaster supervillin, transcript variant L (Svil), mRNA.

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CloneID	OFa17295
Clone ID Related Accession (Same CDS sequence)	NM_001202101.1 , NM_001259637.1 , NM_206242.3 , NM_001274398.2
Accession Version	NM_206242.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	supervillin, isoform L
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_206242.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCAGCG GCAACTCGGG CGGCAGCAGT GGCAATCCCA AGCAGCAGCA GCAGCAGCAG 
CAGCAACAAC AGCTAGAGCA GCTGGGACAC CAGACACATC TTGTGCTAGG AGCATCAAAG 
GAGTTGCCCG CGGTCAGCAG CAGCAGCGAT GAAGAGGGAT CCCTGCGGAC TCTCCACTCC 
AGTCGCAAGG TGACCAATCT GAATGCCAAG TCCACCTCTC TGCCACCCGA GGAAGGACTC 
TCGGGTGATG TCTCCCTGGA GTCGTCCTCC AACCGACTGA ATCTGCGATT GGCCAGGAGC 
CTGGAGCCCT CAGTCCGCAA TATCACTGCA TCCGCCTCCA AGGAAGTACA GAGCTATGAT 
GCTGGGGAGC CTGTGAATAC CAATCGATTG TTTGTGGAAC CGCCCAAGAT ACCACTGTCT 
GCCATCCGAA ACTCTCTTGA CGATCACGAC CTGGATATTG AGGAGTGCGA CAGCACCACC 
CAGTTCGATC AGGGGGATAT CTACGATACC CTGGAACGCT GGAGCCATGG TGTCAGCTTG 
GGGGCACATC TATTGAACTG CGCGAATGGC CACGATCAAT CTGATCCCGA GGACGACCAT 
CAAACCGATC ATCATCAGCA TGCACATCAA TCATTGCCAG TAGAACCCAA GTCTCATCAT 
CATCAGCACC ACCAGGCCAC CAATGCGCGC AGATTCATAA CGCGCACCAC GCGTTCCGCC 
AGTCGCCAGA GTTCCGCCGG ACACTCCCCA CCTCCGCCGC TGCATCGCAG TGACGACTCC 
TCCAGCAGTT CACCCTCACC GACACGCAGA CGGAATAAGC CAGGTGGTGC TCCTGCCGCC 
ACAGATCCAC CATCATCGTC GGCGAACGGG AATAGTTCCG CTTATGAGAC GGCAGCCACC 
ACTGTGATGA CCCATGACCA GCAGCGAGAT CCTCCAGTTG AAGTTCCTAA GGCTCTAAAC 
GTCGAGCCAC CGCAGCCAGA AGACATCGTC CTGGGCTCCT GCCCCACTGC ATCTGCCCAC 
TCAGCAGAGA GCCAAAGTCG AGGAAAAGCT GGCAAATGCA GTATTAATTG CACCTACAGC 
CACAGCCACA GCACCAACAA CAACTGCAAC GCGCGACAAC GCAACAGCAG CAACATCAGC 
AGCAACAACA CCATCAGCAG CAACAGCAGC AGCAGCAAAA GCACTTGCAA CAACAATAGC 
AAACAGCAAC ACGCCACGCC CATGCCGCCC CTAGCTAATG CCAACAAAGC CCCGTTAATT 
GTGCTTAAGA CAGCCGCATC TGCAGCAGCA TTGCCGTCCA TTGCCGCAGC AGCAACATCC 
AGCGGCACGG CAGCAACATC TGGTGGTGTT GGTTCGCCCA TCAAGCGTCG CAAGAATCCG 
CTGTCGCCGC TGCTCCTCGG CAGCCAGTGC CAGTCCACCA TTGCGCCTCC GCAGGCCATT 
GCCAGTCCGC TCCTGAGCGG CGGCAGCTGC TCCTCCTCGA CCACCTCGCC CCAATCCTCG 
CCAGCGGTTC GCACCACCAA GGCGAGTCGT CTGCGCGCCG CCGCGCTTGA TAAAAAGAAG 
GAAGAGGAGC AACGCAATCG CTACAGTTCG CCCGTTTCAC CAAAAGCCTC CAGCAACGGC 
GGAGTCCAAG CACAACGTCG TCTTAGCAAC TCGGAGCTAT CGCCCAAGTC GCCAGGTGCC 
AGTGGATTCA CTTCGGAGCC AGTCGCGTCA GCCACAGCGA ACAGAGTCCC GACGAGATCT 
GCCTCCCATC CTGTGGACAC ACCGCCCACA CAAAGGCGAC AGCCTCTGGT GGCCGTGAGT 
TCGGGCATCT CCATGAAGCA GAGTCTCAGC GAGCTGCACT TCACGGGTGG AGCAGTCAGT 
CCGCCCAAGG CTACGGCGGA ATGCACTCTA AGGATGCGAC CACGTAGCTC CACGATGAGC 
ACCGAGAACA CGGAGAATGG CCGACGTGAC CACTTGGCTA ATCTGCAACT AAGTCCTGTG 
CAAAAGCAAA AGCGAACGCC CGAAGTATCA CCACGCCGTT TCGGAGATCG CGACAAGTCG 
GCCAAACGGA AATCGAATTT GAATCGTTCG TTGACCGAGG AAGCCACCAA GGATGGCGAT 
GAGGTTGCCT CTTCGACCCG CCGGCGCCGA CGTCGTGAAC TGGGAATGGC TCGTCCCCTG 
GTTCCGGGCG ATGATGACGC CCTAAGAGAT TTGCTAACGC CGCAGAAGAG TTCCTGCAGC 
GAGTCAACCA ACTCCATATC CGAACCCCAT CTCCAGTTCA TACCAGCCGT TCGTCATCCG 
GTGGTTCCAG TACGCACCAT GACTGCTGTG CTGCCTGCAC CACGACCGCT GCATACTCCT 
GCGGCCCGCA ACGTCAGCCA GAAGTTCAAT GAAAGGACCA AAACAGATCT GGCCGAGATC 
AAGAAGATTG CTGAGCAATC GGAGGCGCAG GTGCAACAGC CTTCGGTGGA TGATAACCAG 
AAAATGTCAG CCATAATGCG CAGGAAGAGC ACGGATGAGG GTGGATCAGC TTTGGGGGCA 
ACAAACCAGA ATTCACCACC TGCCCCAAAC CAAATTGTAT CCATACTGAA GAAGAAGGAA 
CATGGACTTG GCGAGTGCAA CTCCAGTGCT TCAAGCAATC CCTCGCCGGT GACCTTCTCT 
TCTAGCGTGG TGGATCATCC TTTGGCTGGA GCTGGCCGGT GCAAACGGCA GGGAATACTC 
AAGAAGAGAT CGAGTCTAGA TGAATCGCGC TACTACTCCC GATCGCATTC ACCCGACGAA 
CGTAGTATTC TCATTAAGTC GGCCAGGAGG AATTCCCTGG AGGAAGCTGG CACTGGATTG 
AGTCCGGCTC AGGCCCATGG AATCCTCAAG CAGAGCAGCT ACGACAGCAG CAAGAGCGAC 
GGCTGCCCTT CGGCCACGGA GAGCCAGCCA CACAGTATCC TGAAAAAGAA GGATTCGCTA 
TCGACACCTT CGGACGGAGG ATGCCACAAG CATGTCTCTA TATCGCAAGC TGTAACTTTA 
GCTGCTGCTG AACTGGCTGC CCATGATGGA ATCACCTTCG ATGATGGTGA GGAGCATGAG 
ATACGACCGA TCCTAAAGCA GGAGAGCACC TCCAGCGAGG AGGCTGTGCG TCCGCCAAAG 
CCCATACTCA AAAAGAAATC CTTTGGCGAG GCGGATGAAC ACGAAATACG TCCTATATTG 
AAGAGCTCTC GTAAAAGCAG TCGAGAGGAG TTCGATTTGA GTGGCTTGGA AAACGAGGTC 
AACGATTCCC TATCGTCAAT CCTAAAGACA GACTCGCCGT CCAAGCGTCG GTCGCTCGGT 
TCAGCTTTGC ATGATTTGGA GGAGTCCACC ACTTCCCCGA GCATGCTCAA GAGGCGCACC 
CGTTCGCTGG AACGTCAAGA TGTCCAGCCT GGCATGGATT TGGCAGCTGC ACTGGATGCC 
ATCGCCACAT CACAGGCTAC TCCCAGCACC CCGGTGGACT TCGTGACCAC ACCAACAAGT 
ATATCGGTGG CGGAGCGGAT CAAGCGCATG GAGATGCTGT CGACGCCCAC TTCCCGTGCG 
GCGGGTGGGG CCAACAGCAG CTGGGAATCG CCACTGCAGG CATCGTCAGC AGAGCATGGA 
GCACCCACCA CCCCAAGGGT TCTCAAGCCA AGTGTGCTGC GCAGAGATTT GCAGAGGGAG 
CGGTACAAAA CGCAACCCGT GACCAACGAG GAGATGAGCT TCTTCAAAGC CAACTCCGCG 
CCCTTCGACA TCTCGGCTAC ATCGGCCTTC AAGCCCACCA AGCCGCTGGA CATCCGCTTG 
ACCCACGCAC CGCATGCACC GCTGATTTCG CCGATCACCG GACAGCCACT CAGCCACGTG 
CCCGCGCCAC TGCTGCTATC CTCGTCGACG AACTCGGGAG CGGGATCCTC GTTCCGTCTG 
GCCCGCAGCT CCACCCAACC GCTGCAGTCG CCGCGAGTTG TCCACCTGGC CGGTCAGGCT 
ACTGGTGGCT CCGCTTTGGC ACGCCAGAAC TCGGTGAACG ACAGCATCAA TCTCAGCGAG 
CAGCTAACCC TATCCATCGG CACGGACAGC GAGCACATCT TTGAGATGTC CGCTTTGGAG 
GAGGACTCGG CTATTCAGTC GCTGAGCGAC GAGACAGCCG CTTCCGCATC TGCCAACTCA 
ACATCCACGA CCACTGCAAC CACCATGACA CCGCCCAGTG GCCTGACGCG GAGCAACAGC 
GTTCGGGCCA GGGCCAATAT GTTCCAGCAA TTGCAGGAGC AGTCGCGCAA CCAACGGGCA 
ACGGGAACCA CCAACTCCCG TCAATCTCCA CCAGCGTCGT CGGCGGAGGT GTCTTCAAGT 
GCAGCCGACA ATTCCATGCA CAGTGACGAG CGGTCCACGC CAAATGCAAC GATAGATGCG 
GAATTCGAAC CCTCATCCCT GTCGCTGGCT GAGCGACTGG CCTTCTTCAG CAATCTGTGC 
GAGAGCGGCG GAGGAGGTGG GAGTACCAGT GCCGGTGGAA GGTACCGCAG TCGCACCAGC 
AGCTATTCAA GGAGTCCGCC CGGCGATCGC ACCACTCCCA GGAGCAGCAC CACCGCCAGC 
AGTGTCAGTA TGACGCCCAC GCCCATGGAT TACTCTCCGA TTCCCCACGA CAAGGAGCCA 
TCTAGTCTCA CGCTGGAGAG CATCATCGAG CCCGAGCAGG AGGAGGTCAA GGAGCAGGTG 
GACCAGGATG AGGTCAAGTT GAGGAAGAAG GAGCCAGTCC AGAGGGAATC TTCCCAGCAC 
AATGGCTTCC ACGTGCTGAC CAGGTCCGCC ACCGAACCGC CGTCGTCCAC CTCGCCCCTG 
CGCATCAGCA TACGGACTGT GGGAAAGCTA GTCCTGCCCG ATACCTTCCG CGGCGATCGC 
AACAATAACA GCAGTAGTAG AAGTGGGAGC AACAGCTGGA GCAACACTTG CATGGTCAAG 
CTGCAGAGCT TGGAGCCCGT TAGCCTGTCC GTTCATTCGC GCACCATTGG TAAGGTTAAG 
TCGCCATTCA TTGAAAAGCA GCAGGAGAAA CCACACACCG AGAAGCTAGC GAACGGAACC 
AGCGACAGTG GCTCCGATTC GGGCAAGGAG AACTGCGCCT CCAACGAGCA GGAACATCTG 
CAGCACCAGC AGTCGCAGGA CGAGGATCGA GCTCCGCCCA GGGTGTCCGA GATGCGGAGG 
AAAATCAGCC GACTGGTGCA ACAACAGCAG CCGCAACCAC AGCCCGTCAA TCGGCGGCAC 
ACCACCGAGA TCACCTCCGT GACCGTTGGA ACGCGTTCGC CCAGTGTGGA CGATCGCTTT 
GCCAAGTACT TTGGTGTTAA GGAGAGCTGC AACAGCACGA CCACCGTGCA TAAAACGCAA 
TCGCTGCCAC CCAGCCAAGT TGAGGCCACC AATGCGCACG TGAAACTACC GCAGATCACT 
ACCGTGGTTT CCAAACGGCG CGAGCTGCTC AAGAGGACGC GACCCCTGTC CATGGATCAC 
TACTCCAGCG GCTGCACCAG TCCCACAGCC ATGCCAAGTC CCAAGAGAGC ACATTCGCCC 
GTCGGCCGGC GGAAGGCCGC CATTTCCAGC ATCGAGGACA TTGAGGTCAC GCCGGATGAA 
CTCCGGGCCG CTGGCAGGAA CTTCAAGCTG TTGTACGGCG AGTTATTGGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995964.2
CDS	1142..6775
Translation	MSSGNSGGSSGNPKQQQQQQQQQQLEQLGHQTHLVLGASKELPAVSSSSDEEGSLRTLHSSRKVTNLNAKSTSLPPEEGLSGDVSLESSSNRLNLRLARSLEPSVRNITASASKEVQSYDAGEPVNTNRLFVEPPKIPLSAIRNSLDDHDLDIEECDSTTQFDQGDIYDTLERWSHGVSLGAHLLNCANGHDQSDPEDDHQTDHHQHAHQSLPVEPKSHHHQHHQATNARRFITRTTRSASRQSSAGHSPPPPLHRSDDSSSSSPSPTRRRNKPGGAPAATDPPSSSANGNSSAYETAATTVMTHDQQRDPPVEVPKALNVEPPQPEDIVLGSCPTASAHSAESQSRGKAGKCSINCTYSHSHSTNNNCNARQRNSSNISSNNTISSNSSSSKSTCNNNSKQQHATPMPPLANANKAPLIVLKTAASAAALPSIAAAATSSGTAATSGGVGSPIKRRKNPLSPLLLGSQCQSTIAPPQAIASPLLSGGSCSSSTTSPQSSPAVRTTKASRLRAAALDKKKEEEQRNRYSSPVSPKASSNGGVQAQRRLSNSELSPKSPGASGFTSEPVASATANRVPTRSASHPVDTPPTQRRQPLVAVSSGISMKQSLSELHFTGGAVSPPKATAECTLRMRPRSSTMSTENTENGRRDHLANLQLSPVQKQKRTPEVSPRRFGDRDKSAKRKSNLNRSLTEEATKDGDEVASSTRRRRRRELGMARPLVPGDDDALRDLLTPQKSSCSESTNSISEPHLQFIPAVRHPVVPVRTMTAVLPAPRPLHTPAARNVSQKFNERTKTDLAEIKKIAEQSEAQVQQPSVDDNQKMSAIMRRKSTDEGGSALGATNQNSPPAPNQIVSILKKKEHGLGECNSSASSNPSPVTFSSSVVDHPLAGAGRCKRQGILKKRSSLDESRYYSRSHSPDERSILIKSARRNSLEEAGTGLSPAQAHGILKQSSYDSSKSDGCPSATESQPHSILKKKDSLSTPSDGGCHKHVSISQAVTLAAAELAAHDGITFDDGEEHEIRPILKQESTSSEEAVRPPKPILKKKSFGEADEHEIRPILKSSRKSSREEFDLSGLENEVNDSLSSILKTDSPSKRRSLGSALHDLEESTTSPSMLKRRTRSLERQDVQPGMDLAAALDAIATSQATPSTPVDFVTTPTSISVAERIKRMEMLSTPTSRAAGGANSSWESPLQASSAEHGAPTTPRVLKPSVLRRDLQRERYKTQPVTNEEMSFFKANSAPFDISATSAFKPTKPLDIRLTHAPHAPLISPITGQPLSHVPAPLLLSSSTNSGAGSSFRLARSSTQPLQSPRVVHLAGQATGGSALARQNSVNDSINLSEQLTLSIGTDSEHIFEMSALEEDSAIQSLSDETAASASANSTSTTTATTMTPPSGLTRSNSVRARANMFQQLQEQSRNQRATGTTNSRQSPPASSAEVSSSAADNSMHSDERSTPNATIDAEFEPSSLSLAERLAFFSNLCESGGGGGSTSAGGRYRSRTSSYSRSPPGDRTTPRSSTTASSVSMTPTPMDYSPIPHDKEPSSLTLESIIEPEQEEVKEQVDQDEVKLRKKEPVQRESSQHNGFHVLTRSATEPPSSTSPLRISIRTVGKLVLPDTFRGDRNNNSSSRSGSNSWSNTCMVKLQSLEPVSLSVHSRTIGKVKSPFIEKQQEKPHTEKLANGTSDSGSDSGKENCASNEQEHLQHQQSQDEDRAPPRVSEMRRKISRLVQQQQPQPQPVNRRHTTEITSVTVGTRSPSVDDRFAKYFGVKESCNSTTTVHKTQSLPPSQVEATNAHVKLPQITTVVSKRRELLKRTRPLSMDHYSSGCTSPTAMPSPKRAHSPVGRRKAAISSIEDIEVTPDELRAAGRNFKLLYGELLG

Target ORF information:

RefSeq Version	NM_206242.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster supervillin, transcript variant L (Svil), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206242.3

ORF Insert Sequence:

ATGAGCAGCG GCAACTCGGG CGGCAGCAGT GGCAATCCCA AGCAGCAGCA GCAGCAGCAG 
CAGCAACAAC AGCTAGAGCA GCTGGGACAC CAGACACATC TTGTGCTAGG AGCATCAAAG 
GAGTTGCCCG CGGTCAGCAG CAGCAGCGAT GAAGAGGGAT CCCTGCGGAC TCTCCACTCC 
AGTCGCAAGG TGACCAATCT GAATGCCAAG TCCACCTCTC TGCCACCCGA GGAAGGACTC 
TCGGGTGATG TCTCCCTGGA GTCGTCCTCC AACCGACTGA ATCTGCGATT GGCCAGGAGC 
CTGGAGCCCT CAGTCCGCAA TATCACTGCA TCCGCCTCCA AGGAAGTACA GAGCTATGAT 
GCTGGGGAGC CTGTGAATAC CAATCGATTG TTTGTGGAAC CGCCCAAGAT ACCACTGTCT 
GCCATCCGAA ACTCTCTTGA CGATCACGAC CTGGATATTG AGGAGTGCGA CAGCACCACC 
CAGTTCGATC AGGGGGATAT CTACGATACC CTGGAACGCT GGAGCCATGG TGTCAGCTTG 
GGGGCACATC TATTGAACTG CGCGAATGGC CACGATCAAT CTGATCCCGA GGACGACCAT 
CAAACCGATC ATCATCAGCA TGCACATCAA TCATTGCCAG TAGAACCCAA GTCTCATCAT 
CATCAGCACC ACCAGGCCAC CAATGCGCGC AGATTCATAA CGCGCACCAC GCGTTCCGCC 
AGTCGCCAGA GTTCCGCCGG ACACTCCCCA CCTCCGCCGC TGCATCGCAG TGACGACTCC 
TCCAGCAGTT CACCCTCACC GACACGCAGA CGGAATAAGC CAGGTGGTGC TCCTGCCGCC 
ACAGATCCAC CATCATCGTC GGCGAACGGG AATAGTTCCG CTTATGAGAC GGCAGCCACC 
ACTGTGATGA CCCATGACCA GCAGCGAGAT CCTCCAGTTG AAGTTCCTAA GGCTCTAAAC 
GTCGAGCCAC CGCAGCCAGA AGACATCGTC CTGGGCTCCT GCCCCACTGC ATCTGCCCAC 
TCAGCAGAGA GCCAAAGTCG AGGAAAAGCT GGCAAATGCA GTATTAATTG CACCTACAGC 
CACAGCCACA GCACCAACAA CAACTGCAAC GCGCGACAAC GCAACAGCAG CAACATCAGC 
AGCAACAACA CCATCAGCAG CAACAGCAGC AGCAGCAAAA GCACTTGCAA CAACAATAGC 
AAACAGCAAC ACGCCACGCC CATGCCGCCC CTAGCTAATG CCAACAAAGC CCCGTTAATT 
GTGCTTAAGA CAGCCGCATC TGCAGCAGCA TTGCCGTCCA TTGCCGCAGC AGCAACATCC 
AGCGGCACGG CAGCAACATC TGGTGGTGTT GGTTCGCCCA TCAAGCGTCG CAAGAATCCG 
CTGTCGCCGC TGCTCCTCGG CAGCCAGTGC CAGTCCACCA TTGCGCCTCC GCAGGCCATT 
GCCAGTCCGC TCCTGAGCGG CGGCAGCTGC TCCTCCTCGA CCACCTCGCC CCAATCCTCG 
CCAGCGGTTC GCACCACCAA GGCGAGTCGT CTGCGCGCCG CCGCGCTTGA TAAAAAGAAG 
GAAGAGGAGC AACGCAATCG CTACAGTTCG CCCGTTTCAC CAAAAGCCTC CAGCAACGGC 
GGAGTCCAAG CACAACGTCG TCTTAGCAAC TCGGAGCTAT CGCCCAAGTC GCCAGGTGCC 
AGTGGATTCA CTTCGGAGCC AGTCGCGTCA GCCACAGCGA ACAGAGTCCC GACGAGATCT 
GCCTCCCATC CTGTGGACAC ACCGCCCACA CAAAGGCGAC AGCCTCTGGT GGCCGTGAGT 
TCGGGCATCT CCATGAAGCA GAGTCTCAGC GAGCTGCACT TCACGGGTGG AGCAGTCAGT 
CCGCCCAAGG CTACGGCGGA ATGCACTCTA AGGATGCGAC CACGTAGCTC CACGATGAGC 
ACCGAGAACA CGGAGAATGG CCGACGTGAC CACTTGGCTA ATCTGCAACT AAGTCCTGTG 
CAAAAGCAAA AGCGAACGCC CGAAGTATCA CCACGCCGTT TCGGAGATCG CGACAAGTCG 
GCCAAACGGA AATCGAATTT GAATCGTTCG TTGACCGAGG AAGCCACCAA GGATGGCGAT 
GAGGTTGCCT CTTCGACCCG CCGGCGCCGA CGTCGTGAAC TGGGAATGGC TCGTCCCCTG 
GTTCCGGGCG ATGATGACGC CCTAAGAGAT TTGCTAACGC CGCAGAAGAG TTCCTGCAGC 
GAGTCAACCA ACTCCATATC CGAACCCCAT CTCCAGTTCA TACCAGCCGT TCGTCATCCG 
GTGGTTCCAG TACGCACCAT GACTGCTGTG CTGCCTGCAC CACGACCGCT GCATACTCCT 
GCGGCCCGCA ACGTCAGCCA GAAGTTCAAT GAAAGGACCA AAACAGATCT GGCCGAGATC 
AAGAAGATTG CTGAGCAATC GGAGGCGCAG GTGCAACAGC CTTCGGTGGA TGATAACCAG 
AAAATGTCAG CCATAATGCG CAGGAAGAGC ACGGATGAGG GTGGATCAGC TTTGGGGGCA 
ACAAACCAGA ATTCACCACC TGCCCCAAAC CAAATTGTAT CCATACTGAA GAAGAAGGAA 
CATGGACTTG GCGAGTGCAA CTCCAGTGCT TCAAGCAATC CCTCGCCGGT GACCTTCTCT 
TCTAGCGTGG TGGATCATCC TTTGGCTGGA GCTGGCCGGT GCAAACGGCA GGGAATACTC 
AAGAAGAGAT CGAGTCTAGA TGAATCGCGC TACTACTCCC GATCGCATTC ACCCGACGAA 
CGTAGTATTC TCATTAAGTC GGCCAGGAGG AATTCCCTGG AGGAAGCTGG CACTGGATTG 
AGTCCGGCTC AGGCCCATGG AATCCTCAAG CAGAGCAGCT ACGACAGCAG CAAGAGCGAC 
GGCTGCCCTT CGGCCACGGA GAGCCAGCCA CACAGTATCC TGAAAAAGAA GGATTCGCTA 
TCGACACCTT CGGACGGAGG ATGCCACAAG CATGTCTCTA TATCGCAAGC TGTAACTTTA 
GCTGCTGCTG AACTGGCTGC CCATGATGGA ATCACCTTCG ATGATGGTGA GGAGCATGAG 
ATACGACCGA TCCTAAAGCA GGAGAGCACC TCCAGCGAGG AGGCTGTGCG TCCGCCAAAG 
CCCATACTCA AAAAGAAATC CTTTGGCGAG GCGGATGAAC ACGAAATACG TCCTATATTG 
AAGAGCTCTC GTAAAAGCAG TCGAGAGGAG TTCGATTTGA GTGGCTTGGA AAACGAGGTC 
AACGATTCCC TATCGTCAAT CCTAAAGACA GACTCGCCGT CCAAGCGTCG GTCGCTCGGT 
TCAGCTTTGC ATGATTTGGA GGAGTCCACC ACTTCCCCGA GCATGCTCAA GAGGCGCACC 
CGTTCGCTGG AACGTCAAGA TGTCCAGCCT GGCATGGATT TGGCAGCTGC ACTGGATGCC 
ATCGCCACAT CACAGGCTAC TCCCAGCACC CCGGTGGACT TCGTGACCAC ACCAACAAGT 
ATATCGGTGG CGGAGCGGAT CAAGCGCATG GAGATGCTGT CGACGCCCAC TTCCCGTGCG 
GCGGGTGGGG CCAACAGCAG CTGGGAATCG CCACTGCAGG CATCGTCAGC AGAGCATGGA 
GCACCCACCA CCCCAAGGGT TCTCAAGCCA AGTGTGCTGC GCAGAGATTT GCAGAGGGAG 
CGGTACAAAA CGCAACCCGT GACCAACGAG GAGATGAGCT TCTTCAAAGC CAACTCCGCG 
CCCTTCGACA TCTCGGCTAC ATCGGCCTTC AAGCCCACCA AGCCGCTGGA CATCCGCTTG 
ACCCACGCAC CGCATGCACC GCTGATTTCG CCGATCACCG GACAGCCACT CAGCCACGTG 
CCCGCGCCAC TGCTGCTATC CTCGTCGACG AACTCGGGAG CGGGATCCTC GTTCCGTCTG 
GCCCGCAGCT CCACCCAACC GCTGCAGTCG CCGCGAGTTG TCCACCTGGC CGGTCAGGCT 
ACTGGTGGCT CCGCTTTGGC ACGCCAGAAC TCGGTGAACG ACAGCATCAA TCTCAGCGAG 
CAGCTAACCC TATCCATCGG CACGGACAGC GAGCACATCT TTGAGATGTC CGCTTTGGAG 
GAGGACTCGG CTATTCAGTC GCTGAGCGAC GAGACAGCCG CTTCCGCATC TGCCAACTCA 
ACATCCACGA CCACTGCAAC CACCATGACA CCGCCCAGTG GCCTGACGCG GAGCAACAGC 
GTTCGGGCCA GGGCCAATAT GTTCCAGCAA TTGCAGGAGC AGTCGCGCAA CCAACGGGCA 
ACGGGAACCA CCAACTCCCG TCAATCTCCA CCAGCGTCGT CGGCGGAGGT GTCTTCAAGT 
GCAGCCGACA ATTCCATGCA CAGTGACGAG CGGTCCACGC CAAATGCAAC GATAGATGCG 
GAATTCGAAC CCTCATCCCT GTCGCTGGCT GAGCGACTGG CCTTCTTCAG CAATCTGTGC 
GAGAGCGGCG GAGGAGGTGG GAGTACCAGT GCCGGTGGAA GGTACCGCAG TCGCACCAGC 
AGCTATTCAA GGAGTCCGCC CGGCGATCGC ACCACTCCCA GGAGCAGCAC CACCGCCAGC 
AGTGTCAGTA TGACGCCCAC GCCCATGGAT TACTCTCCGA TTCCCCACGA CAAGGAGCCA 
TCTAGTCTCA CGCTGGAGAG CATCATCGAG CCCGAGCAGG AGGAGGTCAA GGAGCAGGTG 
GACCAGGATG AGGTCAAGTT GAGGAAGAAG GAGCCAGTCC AGAGGGAATC TTCCCAGCAC 
AATGGCTTCC ACGTGCTGAC CAGGTCCGCC ACCGAACCGC CGTCGTCCAC CTCGCCCCTG 
CGCATCAGCA TACGGACTGT GGGAAAGCTA GTCCTGCCCG ATACCTTCCG CGGCGATCGC 
AACAATAACA GCAGTAGTAG AAGTGGGAGC AACAGCTGGA GCAACACTTG CATGGTCAAG 
CTGCAGAGCT TGGAGCCCGT TAGCCTGTCC GTTCATTCGC GCACCATTGG TAAGGTTAAG 
TCGCCATTCA TTGAAAAGCA GCAGGAGAAA CCACACACCG AGAAGCTAGC GAACGGAACC 
AGCGACAGTG GCTCCGATTC GGGCAAGGAG AACTGCGCCT CCAACGAGCA GGAACATCTG 
CAGCACCAGC AGTCGCAGGA CGAGGATCGA GCTCCGCCCA GGGTGTCCGA GATGCGGAGG 
AAAATCAGCC GACTGGTGCA ACAACAGCAG CCGCAACCAC AGCCCGTCAA TCGGCGGCAC 
ACCACCGAGA TCACCTCCGT GACCGTTGGA ACGCGTTCGC CCAGTGTGGA CGATCGCTTT 
GCCAAGTACT TTGGTGTTAA GGAGAGCTGC AACAGCACGA CCACCGTGCA TAAAACGCAA 
TCGCTGCCAC CCAGCCAAGT TGAGGCCACC AATGCGCACG TGAAACTACC GCAGATCACT 
ACCGTGGTTT CCAAACGGCG CGAGCTGCTC AAGAGGACGC GACCCCTGTC CATGGATCAC 
TACTCCAGCG GCTGCACCAG TCCCACAGCC ATGCCAAGTC CCAAGAGAGC ACATTCGCCC 
GTCGGCCGGC GGAAGGCCGC CATTTCCAGC ATCGAGGACA TTGAGGTCAC GCCGGATGAA 
CTCCGGGCCG CTGGCAGGAA CTTCAAGCTG TTGTACGGCG AGTTATTGGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Svil ( NM_001274398.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261327.1 Drosophila melanogaster supervillin, transcript variant T (Svil), mRNA.

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CloneID	OFa17295
Clone ID Related Accession (Same CDS sequence)	NM_001202101.1 , NM_001259637.1 , NM_206242.3 , NM_001274398.2
Accession Version	NM_001274398.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	supervillin, isoform T
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001274398.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCAGCG GCAACTCGGG CGGCAGCAGT GGCAATCCCA AGCAGCAGCA GCAGCAGCAG 
CAGCAACAAC AGCTAGAGCA GCTGGGACAC CAGACACATC TTGTGCTAGG AGCATCAAAG 
GAGTTGCCCG CGGTCAGCAG CAGCAGCGAT GAAGAGGGAT CCCTGCGGAC TCTCCACTCC 
AGTCGCAAGG TGACCAATCT GAATGCCAAG TCCACCTCTC TGCCACCCGA GGAAGGACTC 
TCGGGTGATG TCTCCCTGGA GTCGTCCTCC AACCGACTGA ATCTGCGATT GGCCAGGAGC 
CTGGAGCCCT CAGTCCGCAA TATCACTGCA TCCGCCTCCA AGGAAGTACA GAGCTATGAT 
GCTGGGGAGC CTGTGAATAC CAATCGATTG TTTGTGGAAC CGCCCAAGAT ACCACTGTCT 
GCCATCCGAA ACTCTCTTGA CGATCACGAC CTGGATATTG AGGAGTGCGA CAGCACCACC 
CAGTTCGATC AGGGGGATAT CTACGATACC CTGGAACGCT GGAGCCATGG TGTCAGCTTG 
GGGGCACATC TATTGAACTG CGCGAATGGC CACGATCAAT CTGATCCCGA GGACGACCAT 
CAAACCGATC ATCATCAGCA TGCACATCAA TCATTGCCAG TAGAACCCAA GTCTCATCAT 
CATCAGCACC ACCAGGCCAC CAATGCGCGC AGATTCATAA CGCGCACCAC GCGTTCCGCC 
AGTCGCCAGA GTTCCGCCGG ACACTCCCCA CCTCCGCCGC TGCATCGCAG TGACGACTCC 
TCCAGCAGTT CACCCTCACC GACACGCAGA CGGAATAAGC CAGGTGGTGC TCCTGCCGCC 
ACAGATCCAC CATCATCGTC GGCGAACGGG AATAGTTCCG CTTATGAGAC GGCAGCCACC 
ACTGTGATGA CCCATGACCA GCAGCGAGAT CCTCCAGTTG AAGTTCCTAA GGCTCTAAAC 
GTCGAGCCAC CGCAGCCAGA AGACATCGTC CTGGGCTCCT GCCCCACTGC ATCTGCCCAC 
TCAGCAGAGA GCCAAAGTCG AGGAAAAGCT GGCAAATGCA GTATTAATTG CACCTACAGC 
CACAGCCACA GCACCAACAA CAACTGCAAC GCGCGACAAC GCAACAGCAG CAACATCAGC 
AGCAACAACA CCATCAGCAG CAACAGCAGC AGCAGCAAAA GCACTTGCAA CAACAATAGC 
AAACAGCAAC ACGCCACGCC CATGCCGCCC CTAGCTAATG CCAACAAAGC CCCGTTAATT 
GTGCTTAAGA CAGCCGCATC TGCAGCAGCA TTGCCGTCCA TTGCCGCAGC AGCAACATCC 
AGCGGCACGG CAGCAACATC TGGTGGTGTT GGTTCGCCCA TCAAGCGTCG CAAGAATCCG 
CTGTCGCCGC TGCTCCTCGG CAGCCAGTGC CAGTCCACCA TTGCGCCTCC GCAGGCCATT 
GCCAGTCCGC TCCTGAGCGG CGGCAGCTGC TCCTCCTCGA CCACCTCGCC CCAATCCTCG 
CCAGCGGTTC GCACCACCAA GGCGAGTCGT CTGCGCGCCG CCGCGCTTGA TAAAAAGAAG 
GAAGAGGAGC AACGCAATCG CTACAGTTCG CCCGTTTCAC CAAAAGCCTC CAGCAACGGC 
GGAGTCCAAG CACAACGTCG TCTTAGCAAC TCGGAGCTAT CGCCCAAGTC GCCAGGTGCC 
AGTGGATTCA CTTCGGAGCC AGTCGCGTCA GCCACAGCGA ACAGAGTCCC GACGAGATCT 
GCCTCCCATC CTGTGGACAC ACCGCCCACA CAAAGGCGAC AGCCTCTGGT GGCCGTGAGT 
TCGGGCATCT CCATGAAGCA GAGTCTCAGC GAGCTGCACT TCACGGGTGG AGCAGTCAGT 
CCGCCCAAGG CTACGGCGGA ATGCACTCTA AGGATGCGAC CACGTAGCTC CACGATGAGC 
ACCGAGAACA CGGAGAATGG CCGACGTGAC CACTTGGCTA ATCTGCAACT AAGTCCTGTG 
CAAAAGCAAA AGCGAACGCC CGAAGTATCA CCACGCCGTT TCGGAGATCG CGACAAGTCG 
GCCAAACGGA AATCGAATTT GAATCGTTCG TTGACCGAGG AAGCCACCAA GGATGGCGAT 
GAGGTTGCCT CTTCGACCCG CCGGCGCCGA CGTCGTGAAC TGGGAATGGC TCGTCCCCTG 
GTTCCGGGCG ATGATGACGC CCTAAGAGAT TTGCTAACGC CGCAGAAGAG TTCCTGCAGC 
GAGTCAACCA ACTCCATATC CGAACCCCAT CTCCAGTTCA TACCAGCCGT TCGTCATCCG 
GTGGTTCCAG TACGCACCAT GACTGCTGTG CTGCCTGCAC CACGACCGCT GCATACTCCT 
GCGGCCCGCA ACGTCAGCCA GAAGTTCAAT GAAAGGACCA AAACAGATCT GGCCGAGATC 
AAGAAGATTG CTGAGCAATC GGAGGCGCAG GTGCAACAGC CTTCGGTGGA TGATAACCAG 
AAAATGTCAG CCATAATGCG CAGGAAGAGC ACGGATGAGG GTGGATCAGC TTTGGGGGCA 
ACAAACCAGA ATTCACCACC TGCCCCAAAC CAAATTGTAT CCATACTGAA GAAGAAGGAA 
CATGGACTTG GCGAGTGCAA CTCCAGTGCT TCAAGCAATC CCTCGCCGGT GACCTTCTCT 
TCTAGCGTGG TGGATCATCC TTTGGCTGGA GCTGGCCGGT GCAAACGGCA GGGAATACTC 
AAGAAGAGAT CGAGTCTAGA TGAATCGCGC TACTACTCCC GATCGCATTC ACCCGACGAA 
CGTAGTATTC TCATTAAGTC GGCCAGGAGG AATTCCCTGG AGGAAGCTGG CACTGGATTG 
AGTCCGGCTC AGGCCCATGG AATCCTCAAG CAGAGCAGCT ACGACAGCAG CAAGAGCGAC 
GGCTGCCCTT CGGCCACGGA GAGCCAGCCA CACAGTATCC TGAAAAAGAA GGATTCGCTA 
TCGACACCTT CGGACGGAGG ATGCCACAAG CATGTCTCTA TATCGCAAGC TGTAACTTTA 
GCTGCTGCTG AACTGGCTGC CCATGATGGA ATCACCTTCG ATGATGGTGA GGAGCATGAG 
ATACGACCGA TCCTAAAGCA GGAGAGCACC TCCAGCGAGG AGGCTGTGCG TCCGCCAAAG 
CCCATACTCA AAAAGAAATC CTTTGGCGAG GCGGATGAAC ACGAAATACG TCCTATATTG 
AAGAGCTCTC GTAAAAGCAG TCGAGAGGAG TTCGATTTGA GTGGCTTGGA AAACGAGGTC 
AACGATTCCC TATCGTCAAT CCTAAAGACA GACTCGCCGT CCAAGCGTCG GTCGCTCGGT 
TCAGCTTTGC ATGATTTGGA GGAGTCCACC ACTTCCCCGA GCATGCTCAA GAGGCGCACC 
CGTTCGCTGG AACGTCAAGA TGTCCAGCCT GGCATGGATT TGGCAGCTGC ACTGGATGCC 
ATCGCCACAT CACAGGCTAC TCCCAGCACC CCGGTGGACT TCGTGACCAC ACCAACAAGT 
ATATCGGTGG CGGAGCGGAT CAAGCGCATG GAGATGCTGT CGACGCCCAC TTCCCGTGCG 
GCGGGTGGGG CCAACAGCAG CTGGGAATCG CCACTGCAGG CATCGTCAGC AGAGCATGGA 
GCACCCACCA CCCCAAGGGT TCTCAAGCCA AGTGTGCTGC GCAGAGATTT GCAGAGGGAG 
CGGTACAAAA CGCAACCCGT GACCAACGAG GAGATGAGCT TCTTCAAAGC CAACTCCGCG 
CCCTTCGACA TCTCGGCTAC ATCGGCCTTC AAGCCCACCA AGCCGCTGGA CATCCGCTTG 
ACCCACGCAC CGCATGCACC GCTGATTTCG CCGATCACCG GACAGCCACT CAGCCACGTG 
CCCGCGCCAC TGCTGCTATC CTCGTCGACG AACTCGGGAG CGGGATCCTC GTTCCGTCTG 
GCCCGCAGCT CCACCCAACC GCTGCAGTCG CCGCGAGTTG TCCACCTGGC CGGTCAGGCT 
ACTGGTGGCT CCGCTTTGGC ACGCCAGAAC TCGGTGAACG ACAGCATCAA TCTCAGCGAG 
CAGCTAACCC TATCCATCGG CACGGACAGC GAGCACATCT TTGAGATGTC CGCTTTGGAG 
GAGGACTCGG CTATTCAGTC GCTGAGCGAC GAGACAGCCG CTTCCGCATC TGCCAACTCA 
ACATCCACGA CCACTGCAAC CACCATGACA CCGCCCAGTG GCCTGACGCG GAGCAACAGC 
GTTCGGGCCA GGGCCAATAT GTTCCAGCAA TTGCAGGAGC AGTCGCGCAA CCAACGGGCA 
ACGGGAACCA CCAACTCCCG TCAATCTCCA CCAGCGTCGT CGGCGGAGGT GTCTTCAAGT 
GCAGCCGACA ATTCCATGCA CAGTGACGAG CGGTCCACGC CAAATGCAAC GATAGATGCG 
GAATTCGAAC CCTCATCCCT GTCGCTGGCT GAGCGACTGG CCTTCTTCAG CAATCTGTGC 
GAGAGCGGCG GAGGAGGTGG GAGTACCAGT GCCGGTGGAA GGTACCGCAG TCGCACCAGC 
AGCTATTCAA GGAGTCCGCC CGGCGATCGC ACCACTCCCA GGAGCAGCAC CACCGCCAGC 
AGTGTCAGTA TGACGCCCAC GCCCATGGAT TACTCTCCGA TTCCCCACGA CAAGGAGCCA 
TCTAGTCTCA CGCTGGAGAG CATCATCGAG CCCGAGCAGG AGGAGGTCAA GGAGCAGGTG 
GACCAGGATG AGGTCAAGTT GAGGAAGAAG GAGCCAGTCC AGAGGGAATC TTCCCAGCAC 
AATGGCTTCC ACGTGCTGAC CAGGTCCGCC ACCGAACCGC CGTCGTCCAC CTCGCCCCTG 
CGCATCAGCA TACGGACTGT GGGAAAGCTA GTCCTGCCCG ATACCTTCCG CGGCGATCGC 
AACAATAACA GCAGTAGTAG AAGTGGGAGC AACAGCTGGA GCAACACTTG CATGGTCAAG 
CTGCAGAGCT TGGAGCCCGT TAGCCTGTCC GTTCATTCGC GCACCATTGG TAAGGTTAAG 
TCGCCATTCA TTGAAAAGCA GCAGGAGAAA CCACACACCG AGAAGCTAGC GAACGGAACC 
AGCGACAGTG GCTCCGATTC GGGCAAGGAG AACTGCGCCT CCAACGAGCA GGAACATCTG 
CAGCACCAGC AGTCGCAGGA CGAGGATCGA GCTCCGCCCA GGGTGTCCGA GATGCGGAGG 
AAAATCAGCC GACTGGTGCA ACAACAGCAG CCGCAACCAC AGCCCGTCAA TCGGCGGCAC 
ACCACCGAGA TCACCTCCGT GACCGTTGGA ACGCGTTCGC CCAGTGTGGA CGATCGCTTT 
GCCAAGTACT TTGGTGTTAA GGAGAGCTGC AACAGCACGA CCACCGTGCA TAAAACGCAA 
TCGCTGCCAC CCAGCCAAGT TGAGGCCACC AATGCGCACG TGAAACTACC GCAGATCACT 
ACCGTGGTTT CCAAACGGCG CGAGCTGCTC AAGAGGACGC GACCCCTGTC CATGGATCAC 
TACTCCAGCG GCTGCACCAG TCCCACAGCC ATGCCAAGTC CCAAGAGAGC ACATTCGCCC 
GTCGGCCGGC GGAAGGCCGC CATTTCCAGC ATCGAGGACA TTGAGGTCAC GCCGGATGAA 
CTCCGGGCCG CTGGCAGGAA CTTCAAGCTG TTGTACGGCG AGTTATTGGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261327.1
CDS	1189..6822
Translation	MSSGNSGGSSGNPKQQQQQQQQQQLEQLGHQTHLVLGASKELPAVSSSSDEEGSLRTLHSSRKVTNLNAKSTSLPPEEGLSGDVSLESSSNRLNLRLARSLEPSVRNITASASKEVQSYDAGEPVNTNRLFVEPPKIPLSAIRNSLDDHDLDIEECDSTTQFDQGDIYDTLERWSHGVSLGAHLLNCANGHDQSDPEDDHQTDHHQHAHQSLPVEPKSHHHQHHQATNARRFITRTTRSASRQSSAGHSPPPPLHRSDDSSSSSPSPTRRRNKPGGAPAATDPPSSSANGNSSAYETAATTVMTHDQQRDPPVEVPKALNVEPPQPEDIVLGSCPTASAHSAESQSRGKAGKCSINCTYSHSHSTNNNCNARQRNSSNISSNNTISSNSSSSKSTCNNNSKQQHATPMPPLANANKAPLIVLKTAASAAALPSIAAAATSSGTAATSGGVGSPIKRRKNPLSPLLLGSQCQSTIAPPQAIASPLLSGGSCSSSTTSPQSSPAVRTTKASRLRAAALDKKKEEEQRNRYSSPVSPKASSNGGVQAQRRLSNSELSPKSPGASGFTSEPVASATANRVPTRSASHPVDTPPTQRRQPLVAVSSGISMKQSLSELHFTGGAVSPPKATAECTLRMRPRSSTMSTENTENGRRDHLANLQLSPVQKQKRTPEVSPRRFGDRDKSAKRKSNLNRSLTEEATKDGDEVASSTRRRRRRELGMARPLVPGDDDALRDLLTPQKSSCSESTNSISEPHLQFIPAVRHPVVPVRTMTAVLPAPRPLHTPAARNVSQKFNERTKTDLAEIKKIAEQSEAQVQQPSVDDNQKMSAIMRRKSTDEGGSALGATNQNSPPAPNQIVSILKKKEHGLGECNSSASSNPSPVTFSSSVVDHPLAGAGRCKRQGILKKRSSLDESRYYSRSHSPDERSILIKSARRNSLEEAGTGLSPAQAHGILKQSSYDSSKSDGCPSATESQPHSILKKKDSLSTPSDGGCHKHVSISQAVTLAAAELAAHDGITFDDGEEHEIRPILKQESTSSEEAVRPPKPILKKKSFGEADEHEIRPILKSSRKSSREEFDLSGLENEVNDSLSSILKTDSPSKRRSLGSALHDLEESTTSPSMLKRRTRSLERQDVQPGMDLAAALDAIATSQATPSTPVDFVTTPTSISVAERIKRMEMLSTPTSRAAGGANSSWESPLQASSAEHGAPTTPRVLKPSVLRRDLQRERYKTQPVTNEEMSFFKANSAPFDISATSAFKPTKPLDIRLTHAPHAPLISPITGQPLSHVPAPLLLSSSTNSGAGSSFRLARSSTQPLQSPRVVHLAGQATGGSALARQNSVNDSINLSEQLTLSIGTDSEHIFEMSALEEDSAIQSLSDETAASASANSTSTTTATTMTPPSGLTRSNSVRARANMFQQLQEQSRNQRATGTTNSRQSPPASSAEVSSSAADNSMHSDERSTPNATIDAEFEPSSLSLAERLAFFSNLCESGGGGGSTSAGGRYRSRTSSYSRSPPGDRTTPRSSTTASSVSMTPTPMDYSPIPHDKEPSSLTLESIIEPEQEEVKEQVDQDEVKLRKKEPVQRESSQHNGFHVLTRSATEPPSSTSPLRISIRTVGKLVLPDTFRGDRNNNSSSRSGSNSWSNTCMVKLQSLEPVSLSVHSRTIGKVKSPFIEKQQEKPHTEKLANGTSDSGSDSGKENCASNEQEHLQHQQSQDEDRAPPRVSEMRRKISRLVQQQQPQPQPVNRRHTTEITSVTVGTRSPSVDDRFAKYFGVKESCNSTTTVHKTQSLPPSQVEATNAHVKLPQITTVVSKRRELLKRTRPLSMDHYSSGCTSPTAMPSPKRAHSPVGRRKAAISSIEDIEVTPDELRAAGRNFKLLYGELLG

Target ORF information:

RefSeq Version	NM_001274398.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster supervillin, transcript variant T (Svil), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274398.2

ORF Insert Sequence:

ATGAGCAGCG GCAACTCGGG CGGCAGCAGT GGCAATCCCA AGCAGCAGCA GCAGCAGCAG 
CAGCAACAAC AGCTAGAGCA GCTGGGACAC CAGACACATC TTGTGCTAGG AGCATCAAAG 
GAGTTGCCCG CGGTCAGCAG CAGCAGCGAT GAAGAGGGAT CCCTGCGGAC TCTCCACTCC 
AGTCGCAAGG TGACCAATCT GAATGCCAAG TCCACCTCTC TGCCACCCGA GGAAGGACTC 
TCGGGTGATG TCTCCCTGGA GTCGTCCTCC AACCGACTGA ATCTGCGATT GGCCAGGAGC 
CTGGAGCCCT CAGTCCGCAA TATCACTGCA TCCGCCTCCA AGGAAGTACA GAGCTATGAT 
GCTGGGGAGC CTGTGAATAC CAATCGATTG TTTGTGGAAC CGCCCAAGAT ACCACTGTCT 
GCCATCCGAA ACTCTCTTGA CGATCACGAC CTGGATATTG AGGAGTGCGA CAGCACCACC 
CAGTTCGATC AGGGGGATAT CTACGATACC CTGGAACGCT GGAGCCATGG TGTCAGCTTG 
GGGGCACATC TATTGAACTG CGCGAATGGC CACGATCAAT CTGATCCCGA GGACGACCAT 
CAAACCGATC ATCATCAGCA TGCACATCAA TCATTGCCAG TAGAACCCAA GTCTCATCAT 
CATCAGCACC ACCAGGCCAC CAATGCGCGC AGATTCATAA CGCGCACCAC GCGTTCCGCC 
AGTCGCCAGA GTTCCGCCGG ACACTCCCCA CCTCCGCCGC TGCATCGCAG TGACGACTCC 
TCCAGCAGTT CACCCTCACC GACACGCAGA CGGAATAAGC CAGGTGGTGC TCCTGCCGCC 
ACAGATCCAC CATCATCGTC GGCGAACGGG AATAGTTCCG CTTATGAGAC GGCAGCCACC 
ACTGTGATGA CCCATGACCA GCAGCGAGAT CCTCCAGTTG AAGTTCCTAA GGCTCTAAAC 
GTCGAGCCAC CGCAGCCAGA AGACATCGTC CTGGGCTCCT GCCCCACTGC ATCTGCCCAC 
TCAGCAGAGA GCCAAAGTCG AGGAAAAGCT GGCAAATGCA GTATTAATTG CACCTACAGC 
CACAGCCACA GCACCAACAA CAACTGCAAC GCGCGACAAC GCAACAGCAG CAACATCAGC 
AGCAACAACA CCATCAGCAG CAACAGCAGC AGCAGCAAAA GCACTTGCAA CAACAATAGC 
AAACAGCAAC ACGCCACGCC CATGCCGCCC CTAGCTAATG CCAACAAAGC CCCGTTAATT 
GTGCTTAAGA CAGCCGCATC TGCAGCAGCA TTGCCGTCCA TTGCCGCAGC AGCAACATCC 
AGCGGCACGG CAGCAACATC TGGTGGTGTT GGTTCGCCCA TCAAGCGTCG CAAGAATCCG 
CTGTCGCCGC TGCTCCTCGG CAGCCAGTGC CAGTCCACCA TTGCGCCTCC GCAGGCCATT 
GCCAGTCCGC TCCTGAGCGG CGGCAGCTGC TCCTCCTCGA CCACCTCGCC CCAATCCTCG 
CCAGCGGTTC GCACCACCAA GGCGAGTCGT CTGCGCGCCG CCGCGCTTGA TAAAAAGAAG 
GAAGAGGAGC AACGCAATCG CTACAGTTCG CCCGTTTCAC CAAAAGCCTC CAGCAACGGC 
GGAGTCCAAG CACAACGTCG TCTTAGCAAC TCGGAGCTAT CGCCCAAGTC GCCAGGTGCC 
AGTGGATTCA CTTCGGAGCC AGTCGCGTCA GCCACAGCGA ACAGAGTCCC GACGAGATCT 
GCCTCCCATC CTGTGGACAC ACCGCCCACA CAAAGGCGAC AGCCTCTGGT GGCCGTGAGT 
TCGGGCATCT CCATGAAGCA GAGTCTCAGC GAGCTGCACT TCACGGGTGG AGCAGTCAGT 
CCGCCCAAGG CTACGGCGGA ATGCACTCTA AGGATGCGAC CACGTAGCTC CACGATGAGC 
ACCGAGAACA CGGAGAATGG CCGACGTGAC CACTTGGCTA ATCTGCAACT AAGTCCTGTG 
CAAAAGCAAA AGCGAACGCC CGAAGTATCA CCACGCCGTT TCGGAGATCG CGACAAGTCG 
GCCAAACGGA AATCGAATTT GAATCGTTCG TTGACCGAGG AAGCCACCAA GGATGGCGAT 
GAGGTTGCCT CTTCGACCCG CCGGCGCCGA CGTCGTGAAC TGGGAATGGC TCGTCCCCTG 
GTTCCGGGCG ATGATGACGC CCTAAGAGAT TTGCTAACGC CGCAGAAGAG TTCCTGCAGC 
GAGTCAACCA ACTCCATATC CGAACCCCAT CTCCAGTTCA TACCAGCCGT TCGTCATCCG 
GTGGTTCCAG TACGCACCAT GACTGCTGTG CTGCCTGCAC CACGACCGCT GCATACTCCT 
GCGGCCCGCA ACGTCAGCCA GAAGTTCAAT GAAAGGACCA AAACAGATCT GGCCGAGATC 
AAGAAGATTG CTGAGCAATC GGAGGCGCAG GTGCAACAGC CTTCGGTGGA TGATAACCAG 
AAAATGTCAG CCATAATGCG CAGGAAGAGC ACGGATGAGG GTGGATCAGC TTTGGGGGCA 
ACAAACCAGA ATTCACCACC TGCCCCAAAC CAAATTGTAT CCATACTGAA GAAGAAGGAA 
CATGGACTTG GCGAGTGCAA CTCCAGTGCT TCAAGCAATC CCTCGCCGGT GACCTTCTCT 
TCTAGCGTGG TGGATCATCC TTTGGCTGGA GCTGGCCGGT GCAAACGGCA GGGAATACTC 
AAGAAGAGAT CGAGTCTAGA TGAATCGCGC TACTACTCCC GATCGCATTC ACCCGACGAA 
CGTAGTATTC TCATTAAGTC GGCCAGGAGG AATTCCCTGG AGGAAGCTGG CACTGGATTG 
AGTCCGGCTC AGGCCCATGG AATCCTCAAG CAGAGCAGCT ACGACAGCAG CAAGAGCGAC 
GGCTGCCCTT CGGCCACGGA GAGCCAGCCA CACAGTATCC TGAAAAAGAA GGATTCGCTA 
TCGACACCTT CGGACGGAGG ATGCCACAAG CATGTCTCTA TATCGCAAGC TGTAACTTTA 
GCTGCTGCTG AACTGGCTGC CCATGATGGA ATCACCTTCG ATGATGGTGA GGAGCATGAG 
ATACGACCGA TCCTAAAGCA GGAGAGCACC TCCAGCGAGG AGGCTGTGCG TCCGCCAAAG 
CCCATACTCA AAAAGAAATC CTTTGGCGAG GCGGATGAAC ACGAAATACG TCCTATATTG 
AAGAGCTCTC GTAAAAGCAG TCGAGAGGAG TTCGATTTGA GTGGCTTGGA AAACGAGGTC 
AACGATTCCC TATCGTCAAT CCTAAAGACA GACTCGCCGT CCAAGCGTCG GTCGCTCGGT 
TCAGCTTTGC ATGATTTGGA GGAGTCCACC ACTTCCCCGA GCATGCTCAA GAGGCGCACC 
CGTTCGCTGG AACGTCAAGA TGTCCAGCCT GGCATGGATT TGGCAGCTGC ACTGGATGCC 
ATCGCCACAT CACAGGCTAC TCCCAGCACC CCGGTGGACT TCGTGACCAC ACCAACAAGT 
ATATCGGTGG CGGAGCGGAT CAAGCGCATG GAGATGCTGT CGACGCCCAC TTCCCGTGCG 
GCGGGTGGGG CCAACAGCAG CTGGGAATCG CCACTGCAGG CATCGTCAGC AGAGCATGGA 
GCACCCACCA CCCCAAGGGT TCTCAAGCCA AGTGTGCTGC GCAGAGATTT GCAGAGGGAG 
CGGTACAAAA CGCAACCCGT GACCAACGAG GAGATGAGCT TCTTCAAAGC CAACTCCGCG 
CCCTTCGACA TCTCGGCTAC ATCGGCCTTC AAGCCCACCA AGCCGCTGGA CATCCGCTTG 
ACCCACGCAC CGCATGCACC GCTGATTTCG CCGATCACCG GACAGCCACT CAGCCACGTG 
CCCGCGCCAC TGCTGCTATC CTCGTCGACG AACTCGGGAG CGGGATCCTC GTTCCGTCTG 
GCCCGCAGCT CCACCCAACC GCTGCAGTCG CCGCGAGTTG TCCACCTGGC CGGTCAGGCT 
ACTGGTGGCT CCGCTTTGGC ACGCCAGAAC TCGGTGAACG ACAGCATCAA TCTCAGCGAG 
CAGCTAACCC TATCCATCGG CACGGACAGC GAGCACATCT TTGAGATGTC CGCTTTGGAG 
GAGGACTCGG CTATTCAGTC GCTGAGCGAC GAGACAGCCG CTTCCGCATC TGCCAACTCA 
ACATCCACGA CCACTGCAAC CACCATGACA CCGCCCAGTG GCCTGACGCG GAGCAACAGC 
GTTCGGGCCA GGGCCAATAT GTTCCAGCAA TTGCAGGAGC AGTCGCGCAA CCAACGGGCA 
ACGGGAACCA CCAACTCCCG TCAATCTCCA CCAGCGTCGT CGGCGGAGGT GTCTTCAAGT 
GCAGCCGACA ATTCCATGCA CAGTGACGAG CGGTCCACGC CAAATGCAAC GATAGATGCG 
GAATTCGAAC CCTCATCCCT GTCGCTGGCT GAGCGACTGG CCTTCTTCAG CAATCTGTGC 
GAGAGCGGCG GAGGAGGTGG GAGTACCAGT GCCGGTGGAA GGTACCGCAG TCGCACCAGC 
AGCTATTCAA GGAGTCCGCC CGGCGATCGC ACCACTCCCA GGAGCAGCAC CACCGCCAGC 
AGTGTCAGTA TGACGCCCAC GCCCATGGAT TACTCTCCGA TTCCCCACGA CAAGGAGCCA 
TCTAGTCTCA CGCTGGAGAG CATCATCGAG CCCGAGCAGG AGGAGGTCAA GGAGCAGGTG 
GACCAGGATG AGGTCAAGTT GAGGAAGAAG GAGCCAGTCC AGAGGGAATC TTCCCAGCAC 
AATGGCTTCC ACGTGCTGAC CAGGTCCGCC ACCGAACCGC CGTCGTCCAC CTCGCCCCTG 
CGCATCAGCA TACGGACTGT GGGAAAGCTA GTCCTGCCCG ATACCTTCCG CGGCGATCGC 
AACAATAACA GCAGTAGTAG AAGTGGGAGC AACAGCTGGA GCAACACTTG CATGGTCAAG 
CTGCAGAGCT TGGAGCCCGT TAGCCTGTCC GTTCATTCGC GCACCATTGG TAAGGTTAAG 
TCGCCATTCA TTGAAAAGCA GCAGGAGAAA CCACACACCG AGAAGCTAGC GAACGGAACC 
AGCGACAGTG GCTCCGATTC GGGCAAGGAG AACTGCGCCT CCAACGAGCA GGAACATCTG 
CAGCACCAGC AGTCGCAGGA CGAGGATCGA GCTCCGCCCA GGGTGTCCGA GATGCGGAGG 
AAAATCAGCC GACTGGTGCA ACAACAGCAG CCGCAACCAC AGCCCGTCAA TCGGCGGCAC 
ACCACCGAGA TCACCTCCGT GACCGTTGGA ACGCGTTCGC CCAGTGTGGA CGATCGCTTT 
GCCAAGTACT TTGGTGTTAA GGAGAGCTGC AACAGCACGA CCACCGTGCA TAAAACGCAA 
TCGCTGCCAC CCAGCCAAGT TGAGGCCACC AATGCGCACG TGAAACTACC GCAGATCACT 
ACCGTGGTTT CCAAACGGCG CGAGCTGCTC AAGAGGACGC GACCCCTGTC CATGGATCAC 
TACTCCAGCG GCTGCACCAG TCCCACAGCC ATGCCAAGTC CCAAGAGAGC ACATTCGCCC 
GTCGGCCGGC GGAAGGCCGC CATTTCCAGC ATCGAGGACA TTGAGGTCAC GCCGGATGAA 
CTCCGGGCCG CTGGCAGGAA CTTCAAGCTG TTGTACGGCG AGTTATTGGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Svil ( NM_139464.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_647721.3 Drosophila melanogaster supervillin, transcript variant K (Svil), mRNA.

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CloneID	OFa17296
Clone ID Related Accession (Same CDS sequence)	NM_139464.4
Accession Version	NM_139464.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	supervillin, isoform K
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_139464.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGGCCC TCAAGGAGTG CCTCATACGG GAGGGTGTTC TCAATCTGGA CAGCCCCGAG 
AAGTACCGAC CGTACCATCT AAACGGCAGC AAAAGCAGCG GCGCCATGGA TTTTCCCCGA 
TATCGCAAAA CCATTGCGGC CTCAAGTGAC TTATCCACAG CACCTGCCAC TGCCAAGACC 
AGCTGCCAGC CACCTGAGCC ACTGAAACAG AAGCCACCGC AGCAGCAGAC CACCAAGGAC 
TCGGAGGATT CCAGTTCACA GAAGAGTGCT CCAGTCCAGT CGTATCGTGG TCAGCATCCG 
CACTTCCACC ATCACACCAC GCCCGCTTAC TACACGGATC TTCAGCTGAG AATTCACACG 
CCGAACGCAT CGGATGTGGG CGCCTTGAAC GAGAACTATC GAAGTGTATA CAGTACTCCG 
AGCACCCAGC TGGTGGAACA GCGATCGTTT CCAAAGAGCT CCACGGCCAC GAAGGATCCA 
CCGCTGCCCA TAAGCAATCC GGCTAGGGGA ACATTTTTTC GTGGCGACTC TGCGTCCAGC 
GACAGTCACT CAAGACTCCG TCGGAGCCAG CAGAGAACCG GCAGCGAGGG ACGCTTCTTC 
AGATACTCTC AGGGATCTGT GAGCCCTACC AAAGGTGAAC CTCCAATCGG CCACGAAGGT 
GGTGAGCAGC CACCCGTTGT CGCCAGTCAC TCCCCCATCA CATCCACCAG CACCACCACC 
ATCAGTAGTT GTAACCCCGT AGAGCAGGCT AGTCCACCAC CAACAAACGT AGTAAATCCC 
AGCGGATCCG GTCGCAACAG CCGGCACAAG CAGCGTTTCC ACAGTCGATC CCGTCGAGCG 
GTGCGCGTGC GCAGTGAATC GCGTCCGATT AGCGCCCTCT ATGACATAAT ATGCAAGGAG 
AAGAACCTGG AAAACAGCAG CAGCAGCAGC AGCAGCAGCA ACGAGCAGGA GAAGAAAAAG 
GATCAGGAGC AGCCTAAGAC CACAACCGAC AAGCTGGCGA CCTTTTGGCC CCTGCACCAT 
CACTACCTCA ATCCCCCGAT GAGCAGCGGC AACTCGGGCG GCAGCAGTGG CAATCCCAAG 
CAGCAGCAGC AGCAGCAGCA GCAACAACAG CTAGAGCAGC TGGGACACCA GACACATCTT 
GTGCTAGGAG CATCAAAGGA GTTGCCCGCG GTCAGCAGCA GCAGCGATGA AGAGGGATCC 
CTGCGGACTC TCCACTCCAG TCGCAAGGTG ACCAATCTGA ATGCCAAGTC CACCTCTCTG 
CCACCCGAGG AAGGACTCTC GGGTGATGTC TCCCTGGAGT CGTCCTCCAA CCGACTGAAT 
CTGCGATTGG CCAGGAGCCT GGAGCCCTCA GTCCGCAATA TCACTGCATC CGCCTCCAAG 
GAAGTACAGA GCTATGATGC TGGGGAGCCT GTGAATACCA ATCGATTGTT TGTGGAACCG 
CCCAAGATAC CACTGTCTGC CATCCGAAAC TCTCTTGACG ATCACGACCT GGATATTGAG 
GAGTGCGACA GCACCACCCA GTTCGATCAG GGGGATATCT ACGATACCCT GGAACGCTGG 
AGCCATGGTG TCAGCTTGGG GGCACATCTA TTGAACTGCG CGAATGGCCA CGATCAATCT 
GATCCCGAGG ACGACCATCA AACCGATCAT CATCAGCATG CACATCAATC ATTGCCAGTA 
GAACCCAAGT CTCATCATCA TCAGCACCAC CAGGCCACCA ATGCGCGCAG ATTCATAACG 
CGCACCACGC GTTCCGCCAG TCGCCAGAGT TCCGCCGGAC ACTCCCCACC TCCGCCGCTG 
CATCGCAGTG ACGACTCCTC CAGCAGTTCA CCCTCACCGA CACGCAGACG GAATAAGCCA 
GGTGGTGCTC CTGCCGCCAC AGATCCACCA TCATCGTCGG CGAACGGGAA TAGTTCCGCT 
TATGAGACGG CAGCCACCAC TGTGATGACC CATGACCAGC AGCGAGATCC TCCAGTTGAA 
GTTCCTAAGG CTCTAAACGT CGAGCCACCG CAGCCAGAAG ACATCGTCCT GGGCTCCTGC 
CCCACTGCAT CTGCCCACTC AGCAGAGAGC CAAAGTCGAG GAAAAGCTGG CAAATGCAGT 
ATTAATTGCA CCTACAGCCA CAGCCACAGC ACCAACAACA ACTGCAACGC GCGACAACGC 
AACAGCAGCA ACATCAGCAG CAACAACACC ATCAGCAGCA ACAGCAGCAG CAGCAAAAGC 
ACTTGCAACA ACAATAGCAA ACAGCAACAC GCCACGCCCA TGCCGCCCCT AGCTAATGCC 
AACAAAGCCC CGTTAATTGT GCTTAAGACA GCCGCATCTG CAGCAGCATT GCCGTCCATT 
GCCGCAGCAG CAACATCCAG CGGCACGGCA GCAACATCTG GTGGTGTTGG TTCGCCCATC 
AAGCGTCGCA AGAATCCGCT GTCGCCGCTG CTCCTCGGCA GCCAGTGCCA GTCCACCATT 
GCGCCTCCGC AGGCCATTGC CAGTCCGCTC CTGAGCGGCG GCAGCTGCTC CTCCTCGACC 
ACCTCGCCCC AATCCTCGCC AGCGGTTCGC ACCACCAAGG CGAGTCGTCT GCGCGCCGCC 
GCGCTTGATA AAAAGAAGGA AGAGGAGCAA CGCAATCGCT ACAGTTCGCC CGTTTCACCA 
AAAGCCTCCA GCAACGGCGG AGTCCAAGCA CAACGTCGTC TTAGCAACTC GGAGCTATCG 
CCCAAGTCGC CAGGTGCCAG TGGATTCACT TCGGAGCCAG TCGCGTCAGC CACAGCGAAC 
AGAGTCCCGA CGAGATCTGC CTCCCATCCT GTGGACACAC CGCCCACACA AAGGCGACAG 
CCTCTGGTGG CCGTGAGTTC GGGCATCTCC ATGAAGCAGA GTCTCAGCGA GCTGCACTTC 
ACGGGTGGAG CAGTCAGTCC GCCCAAGGCT ACGGCGGAAT GCACTCTAAG GATGCGACCA 
CGTAGCTCCA CGATGAGCAC CGAGAACACG GAGAATGGCC GACGTGACCA CTTGGCTAAT 
CTGCAACTAA GTCCTGTGCA AAAGCAAAAG CGAACGCCCG AAGTATCACC ACGCCGTTTC 
GGAGATCGCG ACAAGTCGGC CAAACGGAAA TCGAATTTGA ATCGTTCGTT GACCGAGGAA 
GCCACCAAGG ATGGCGATGA GGTTGCCTCT TCGACCCGCC GGCGCCGACG TCGTGAACTG 
GGAATGGCTC GTCCCCTGGT TCCGGGCGAT GATGACGCCC TAAGAGATTT GCTAACGCCG 
CAGAAGAGTT CCTGCAGCGA GTCAACCAAC TCCATATCCG AACCCCATCT CCAGTTCATA 
CCAGCCGTTC GTCATCCGGT GGTTCCAGTA CGCACCATGA CTGCTGTGCT GCCTGCACCA 
CGACCGCTGC ATACTCCTGC GGCCCGCAAC GTCAGCCAGA AGTTCAATGA AAGGACCAAA 
ACAGATCTGG CCGAGATCAA GAAGATTGCT GAGCAATCGG AGGCGCAGGT GCAACAGCCT 
TCGGTGGATG ATAACCAGAA AATGTCAGCC ATAATGCGCA GGAAGAGCAC GGATGAGGGT 
GGATCAGCTT TGGGGGCAAC AAACCAGAAT TCACCACCTG CCCCAAACCA AATTGTATCC 
ATACTGAAGA AGAAGGAACA TGGACTTGGC GAGTGCAACT CCAGTGCTTC AAGCAATCCC 
TCGCCGGTGA CCTTCTCTTC TAGCGTGGTG GATCATCCTT TGGCTGGAGC TGGCCGGTGC 
AAACGGCAGG GAATACTCAA GAAGAGATCG AGTCTAGATG AATCGCGCTA CTACTCCCGA 
TCGCATTCAC CCGACGAACG TAGTATTCTC ATTAAGTCGG CCAGGAGGAA TTCCCTGGAG 
GAAGCTGGCA CTGGATTGAG TCCGGCTCAG GCCCATGGAA TCCTCAAGCA GAGCAGCTAC 
GACAGCAGCA AGAGCGACGG CTGCCCTTCG GCCACGGAGA GCCAGCCACA CAGTATCCTG 
AAAAAGAAGG ATTCGCTATC GACACCTTCG GACGGAGGAT GCCACAAGCA TGTCTCTATA 
TCGCAAGCTG TAACTTTAGC TGCTGCTGAA CTGGCTGCCC ATGATGGAAT CACCTTCGAT 
GATGGTGAGG AGCATGAGAT ACGACCGATC CTAAAGCAGG AGAGCACCTC CAGCGAGGAG 
GCTGTGCGTC CGCCAAAGCC CATACTCAAA AAGAAATCCT TTGGCGAGGC GGATGAACAC 
GAAATACGTC CTATATTGAA GAGCTCTCGT AAAAGCAGTC GAGAGGAGTT CGATTTGAGT 
GGCTTGGAAA ACGAGGTCAA CGATTCCCTA TCGTCAATCC TAAAGACAGA CTCGCCGTCC 
AAGCGTCGGT CGCTCGGTTC AGCTTTGCAT GATTTGGAGG AGTCCACCAC TTCCCCGAGC 
ATGCTCAAGA GGCGCACCCG TTCGCTGGAA CGTCAAGATG TCCAGCCTGG CATGGATTTG 
GCAGCTGCAC TGGATGCCAT CGCCACATCA CAGGCTACTC CCAGCACCCC GGTGGACTTC 
GTGACCACAC CAACAAGTAT ATCGGTGGCG GAGCGGATCA AGCGCATGGA GATGCTGTCG 
ACGCCCACTT CCCGTGCGGC GGGTGGGGCC AACAGCAGCT GGGAATCGCC ACTGCAGGCA 
TCGTCAGCAG AGCATGGAGC ACCCACCACC CCAAGGGTTC TCAAGCCAAG TGTGCTGCGC 
AGAGATTTGC AGAGGGAGCG GTACAAAACG CAACCCGTGA CCAACGAGGA GATGAGCTTC 
TTCAAAGCCA ACTCCGCGCC CTTCGACATC TCGGCTACAT CGGCCTTCAA GCCCACCAAG 
CCGCTGGACA TCCGCTTGAC CCACGCACCG CATGCACCGC TGATTTCGCC GATCACCGGA 
CAGCCACTCA GCCACGTGCC CGCGCCACTG CTGCTATCCT CGTCGACGAA CTCGGGAGCG 
GGATCCTCGT TCCGTCTGGC CCGCAGCTCC ACCCAACCGC TGCAGTCGCC GCGAGTTGTC 
CACCTGGCCG GTCAGGCTAC TGGTGGCTCC GCTTTGGCAC GCCAGAACTC GGTGAACGAC 
AGCATCAATC TCAGCGAGCA GCTAACCCTA TCCATCGGCA CGGACAGCGA GCACATCTTT 
GAGATGTCCG CTTTGGAGGA GGACTCGGCT ATTCAGTCGC TGAGCGACGA GACAGCCGCT 
TCCGCATCTG CCAACTCAAC ATCCACGACC ACTGCAACCA CCATGACACC GCCCAGTGGC 
CTGACGCGGA GCAACAGCGT TCGGGCCAGG GCCAATATGT TCCAGCAATT GCAGGAGCAG 
TCGCGCAACC AACGGGCAAC GGGAACCACC AACTCCCGTC AATCTCCACC AGCGTCGTCG 
GCGGAGGTGT CTTCAAGTGC AGCCGACAAT TCCATGCACA GTGACGAGCG GTCCACGCCA 
AATGCAACGA TAGATGCGGA ATTCGAACCC TCATCCCTGT CGCTGGCTGA GCGACTGGCC 
TTCTTCAGCA ATCTGTGCGA GAGCGGCGGA GGAGGTGGGA GTACCAGTGC CGGTGGAAGG 
TACCGCAGTC GCACCAGCAG CTATTCAAGG AGTCCGCCCG GCGATCGCAC CACTCCCAGG 
AGCAGCACCA CCGCCAGCAG TGTCAGTATG ACGCCCACGC CCATGGATTA CTCTCCGATT 
CCCCACGACA AGGAGCCATC TAGTCTCACG CTGGAGAGCA TCATCGAGCC CGAGCAGGAG 
GAGGTCAAGG AGCAGGTGGA CCAGGATGAG GTCAAGTTGA GGAAGAAGGA GCCAGTCCAG 
AGGGAATCTT CCCAGCACAA TGGCTTCCAC GTGCTGACCA GGTCCGCCAC CGAACCGCCG 
TCGTCCACCT CGCCCCTGCG CATCAGCATA CGGACTGTGG GAAAGCTAGT CCTGCCCGAT 
ACCTTCCGCG GCGATCGCAA CAATAACAGC AGTAGTAGAA GTGGGAGCAA CAGCTGGAGC 
AACACTTGCA TGGTCAAGCT GCAGAGCTTG GAGCCCGTTA GCCTGTCCGT TCATTCGCGC 
ACCATTGGTA AGGTTAAGTC GCCATTCATT GAAAAGCAGC AGGAGAAACC ACACACCGAG 
AAGCTAGCGA ACGGAACCAG CGACAGTGGC TCCGATTCGG GCAAGGAGAA CTGCGCCTCC 
AACGAGCAGG AACATCTGCA GCACCAGCAG TCGCAGGACG AGGATCGAGC TCCGCCCAGG 
GTGTCCGAGA TGCGGAGGAA AATCAGCCGA CTGGTGCAAC AACAGCAGCC GCAACCACAG 
CCCGTCAATC GGCGGCACAC CACCGAGATC ACCTCCGTGA CCGTTGGAAC GCGTTCGCCC 
AGTGTGGACG ATCGCTTTGC CAAGTACTTT GGTGTTAAGG AGAGCTGCAA CAGCACGACC 
ACCGTGCATA AAACGCAATC GCTGCCACCC AGCCAAGTTG AGGCCACCAA TGCGCACGTG 
AAACTACCGC AGATCACTAC CGTGGTTTCC AAACGGCGCG AGCTGCTCAA GAGGACGCGA 
CCCCTGTCCA TGGATCACTA CTCCAGCGGC TGCACCAGTC CCACAGCCAT GCCAAGTCCC 
AAGAGAGCAC ATTCGCCCGT CGGCCGGCGG AAGGCCGCCA TTTCCAGCAT CGAGGACATT 
GAGGTCACGC CGGATGAACT CCGGGCCGCT GGCAGGAACT TCAAGCTGTT GTACGGCGAG 
TTATTGGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_647721.3
CDS	394..7065
Translation	MLALKECLIREGVLNLDSPEKYRPYHLNGSKSSGAMDFPRYRKTIAASSDLSTAPATAKTSCQPPEPLKQKPPQQQTTKDSEDSSSQKSAPVQSYRGQHPHFHHHTTPAYYTDLQLRIHTPNASDVGALNENYRSVYSTPSTQLVEQRSFPKSSTATKDPPLPISNPARGTFFRGDSASSDSHSRLRRSQQRTGSEGRFFRYSQGSVSPTKGEPPIGHEGGEQPPVVASHSPITSTSTTTISSCNPVEQASPPPTNVVNPSGSGRNSRHKQRFHSRSRRAVRVRSESRPISALYDIICKEKNLENSSSSSSSSNEQEKKKDQEQPKTTTDKLATFWPLHHHYLNPPMSSGNSGGSSGNPKQQQQQQQQQQLEQLGHQTHLVLGASKELPAVSSSSDEEGSLRTLHSSRKVTNLNAKSTSLPPEEGLSGDVSLESSSNRLNLRLARSLEPSVRNITASASKEVQSYDAGEPVNTNRLFVEPPKIPLSAIRNSLDDHDLDIEECDSTTQFDQGDIYDTLERWSHGVSLGAHLLNCANGHDQSDPEDDHQTDHHQHAHQSLPVEPKSHHHQHHQATNARRFITRTTRSASRQSSAGHSPPPPLHRSDDSSSSSPSPTRRRNKPGGAPAATDPPSSSANGNSSAYETAATTVMTHDQQRDPPVEVPKALNVEPPQPEDIVLGSCPTASAHSAESQSRGKAGKCSINCTYSHSHSTNNNCNARQRNSSNISSNNTISSNSSSSKSTCNNNSKQQHATPMPPLANANKAPLIVLKTAASAAALPSIAAAATSSGTAATSGGVGSPIKRRKNPLSPLLLGSQCQSTIAPPQAIASPLLSGGSCSSSTTSPQSSPAVRTTKASRLRAAALDKKKEEEQRNRYSSPVSPKASSNGGVQAQRRLSNSELSPKSPGASGFTSEPVASATANRVPTRSASHPVDTPPTQRRQPLVAVSSGISMKQSLSELHFTGGAVSPPKATAECTLRMRPRSSTMSTENTENGRRDHLANLQLSPVQKQKRTPEVSPRRFGDRDKSAKRKSNLNRSLTEEATKDGDEVASSTRRRRRRELGMARPLVPGDDDALRDLLTPQKSSCSESTNSISEPHLQFIPAVRHPVVPVRTMTAVLPAPRPLHTPAARNVSQKFNERTKTDLAEIKKIAEQSEAQVQQPSVDDNQKMSAIMRRKSTDEGGSALGATNQNSPPAPNQIVSILKKKEHGLGECNSSASSNPSPVTFSSSVVDHPLAGAGRCKRQGILKKRSSLDESRYYSRSHSPDERSILIKSARRNSLEEAGTGLSPAQAHGILKQSSYDSSKSDGCPSATESQPHSILKKKDSLSTPSDGGCHKHVSISQAVTLAAAELAAHDGITFDDGEEHEIRPILKQESTSSEEAVRPPKPILKKKSFGEADEHEIRPILKSSRKSSREEFDLSGLENEVNDSLSSILKTDSPSKRRSLGSALHDLEESTTSPSMLKRRTRSLERQDVQPGMDLAAALDAIATSQATPSTPVDFVTTPTSISVAERIKRMEMLSTPTSRAAGGANSSWESPLQASSAEHGAPTTPRVLKPSVLRRDLQRERYKTQPVTNEEMSFFKANSAPFDISATSAFKPTKPLDIRLTHAPHAPLISPITGQPLSHVPAPLLLSSSTNSGAGSSFRLARSSTQPLQSPRVVHLAGQATGGSALARQNSVNDSINLSEQLTLSIGTDSEHIFEMSALEEDSAIQSLSDETAASASANSTSTTTATTMTPPSGLTRSNSVRARANMFQQLQEQSRNQRATGTTNSRQSPPASSAEVSSSAADNSMHSDERSTPNATIDAEFEPSSLSLAERLAFFSNLCESGGGGGSTSAGGRYRSRTSSYSRSPPGDRTTPRSSTTASSVSMTPTPMDYSPIPHDKEPSSLTLESIIEPEQEEVKEQVDQDEVKLRKKEPVQRESSQHNGFHVLTRSATEPPSSTSPLRISIRTVGKLVLPDTFRGDRNNNSSSRSGSNSWSNTCMVKLQSLEPVSLSVHSRTIGKVKSPFIEKQQEKPHTEKLANGTSDSGSDSGKENCASNEQEHLQHQQSQDEDRAPPRVSEMRRKISRLVQQQQPQPQPVNRRHTTEITSVTVGTRSPSVDDRFAKYFGVKESCNSTTTVHKTQSLPPSQVEATNAHVKLPQITTVVSKRRELLKRTRPLSMDHYSSGCTSPTAMPSPKRAHSPVGRRKAAISSIEDIEVTPDELRAAGRNFKLLYGELLG

Target ORF information:

RefSeq Version	NM_139464.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster supervillin, transcript variant K (Svil), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139464.4

ORF Insert Sequence:

ATGCTGGCCC TCAAGGAGTG CCTCATACGG GAGGGTGTTC TCAATCTGGA CAGCCCCGAG 
AAGTACCGAC CGTACCATCT AAACGGCAGC AAAAGCAGCG GCGCCATGGA TTTTCCCCGA 
TATCGCAAAA CCATTGCGGC CTCAAGTGAC TTATCCACAG CACCTGCCAC TGCCAAGACC 
AGCTGCCAGC CACCTGAGCC ACTGAAACAG AAGCCACCGC AGCAGCAGAC CACCAAGGAC 
TCGGAGGATT CCAGTTCACA GAAGAGTGCT CCAGTCCAGT CGTATCGTGG TCAGCATCCG 
CACTTCCACC ATCACACCAC GCCCGCTTAC TACACGGATC TTCAGCTGAG AATTCACACG 
CCGAACGCAT CGGATGTGGG CGCCTTGAAC GAGAACTATC GAAGTGTATA CAGTACTCCG 
AGCACCCAGC TGGTGGAACA GCGATCGTTT CCAAAGAGCT CCACGGCCAC GAAGGATCCA 
CCGCTGCCCA TAAGCAATCC GGCTAGGGGA ACATTTTTTC GTGGCGACTC TGCGTCCAGC 
GACAGTCACT CAAGACTCCG TCGGAGCCAG CAGAGAACCG GCAGCGAGGG ACGCTTCTTC 
AGATACTCTC AGGGATCTGT GAGCCCTACC AAAGGTGAAC CTCCAATCGG CCACGAAGGT 
GGTGAGCAGC CACCCGTTGT CGCCAGTCAC TCCCCCATCA CATCCACCAG CACCACCACC 
ATCAGTAGTT GTAACCCCGT AGAGCAGGCT AGTCCACCAC CAACAAACGT AGTAAATCCC 
AGCGGATCCG GTCGCAACAG CCGGCACAAG CAGCGTTTCC ACAGTCGATC CCGTCGAGCG 
GTGCGCGTGC GCAGTGAATC GCGTCCGATT AGCGCCCTCT ATGACATAAT ATGCAAGGAG 
AAGAACCTGG AAAACAGCAG CAGCAGCAGC AGCAGCAGCA ACGAGCAGGA GAAGAAAAAG 
GATCAGGAGC AGCCTAAGAC CACAACCGAC AAGCTGGCGA CCTTTTGGCC CCTGCACCAT 
CACTACCTCA ATCCCCCGAT GAGCAGCGGC AACTCGGGCG GCAGCAGTGG CAATCCCAAG 
CAGCAGCAGC AGCAGCAGCA GCAACAACAG CTAGAGCAGC TGGGACACCA GACACATCTT 
GTGCTAGGAG CATCAAAGGA GTTGCCCGCG GTCAGCAGCA GCAGCGATGA AGAGGGATCC 
CTGCGGACTC TCCACTCCAG TCGCAAGGTG ACCAATCTGA ATGCCAAGTC CACCTCTCTG 
CCACCCGAGG AAGGACTCTC GGGTGATGTC TCCCTGGAGT CGTCCTCCAA CCGACTGAAT 
CTGCGATTGG CCAGGAGCCT GGAGCCCTCA GTCCGCAATA TCACTGCATC CGCCTCCAAG 
GAAGTACAGA GCTATGATGC TGGGGAGCCT GTGAATACCA ATCGATTGTT TGTGGAACCG 
CCCAAGATAC CACTGTCTGC CATCCGAAAC TCTCTTGACG ATCACGACCT GGATATTGAG 
GAGTGCGACA GCACCACCCA GTTCGATCAG GGGGATATCT ACGATACCCT GGAACGCTGG 
AGCCATGGTG TCAGCTTGGG GGCACATCTA TTGAACTGCG CGAATGGCCA CGATCAATCT 
GATCCCGAGG ACGACCATCA AACCGATCAT CATCAGCATG CACATCAATC ATTGCCAGTA 
GAACCCAAGT CTCATCATCA TCAGCACCAC CAGGCCACCA ATGCGCGCAG ATTCATAACG 
CGCACCACGC GTTCCGCCAG TCGCCAGAGT TCCGCCGGAC ACTCCCCACC TCCGCCGCTG 
CATCGCAGTG ACGACTCCTC CAGCAGTTCA CCCTCACCGA CACGCAGACG GAATAAGCCA 
GGTGGTGCTC CTGCCGCCAC AGATCCACCA TCATCGTCGG CGAACGGGAA TAGTTCCGCT 
TATGAGACGG CAGCCACCAC TGTGATGACC CATGACCAGC AGCGAGATCC TCCAGTTGAA 
GTTCCTAAGG CTCTAAACGT CGAGCCACCG CAGCCAGAAG ACATCGTCCT GGGCTCCTGC 
CCCACTGCAT CTGCCCACTC AGCAGAGAGC CAAAGTCGAG GAAAAGCTGG CAAATGCAGT 
ATTAATTGCA CCTACAGCCA CAGCCACAGC ACCAACAACA ACTGCAACGC GCGACAACGC 
AACAGCAGCA ACATCAGCAG CAACAACACC ATCAGCAGCA ACAGCAGCAG CAGCAAAAGC 
ACTTGCAACA ACAATAGCAA ACAGCAACAC GCCACGCCCA TGCCGCCCCT AGCTAATGCC 
AACAAAGCCC CGTTAATTGT GCTTAAGACA GCCGCATCTG CAGCAGCATT GCCGTCCATT 
GCCGCAGCAG CAACATCCAG CGGCACGGCA GCAACATCTG GTGGTGTTGG TTCGCCCATC 
AAGCGTCGCA AGAATCCGCT GTCGCCGCTG CTCCTCGGCA GCCAGTGCCA GTCCACCATT 
GCGCCTCCGC AGGCCATTGC CAGTCCGCTC CTGAGCGGCG GCAGCTGCTC CTCCTCGACC 
ACCTCGCCCC AATCCTCGCC AGCGGTTCGC ACCACCAAGG CGAGTCGTCT GCGCGCCGCC 
GCGCTTGATA AAAAGAAGGA AGAGGAGCAA CGCAATCGCT ACAGTTCGCC CGTTTCACCA 
AAAGCCTCCA GCAACGGCGG AGTCCAAGCA CAACGTCGTC TTAGCAACTC GGAGCTATCG 
CCCAAGTCGC CAGGTGCCAG TGGATTCACT TCGGAGCCAG TCGCGTCAGC CACAGCGAAC 
AGAGTCCCGA CGAGATCTGC CTCCCATCCT GTGGACACAC CGCCCACACA AAGGCGACAG 
CCTCTGGTGG CCGTGAGTTC GGGCATCTCC ATGAAGCAGA GTCTCAGCGA GCTGCACTTC 
ACGGGTGGAG CAGTCAGTCC GCCCAAGGCT ACGGCGGAAT GCACTCTAAG GATGCGACCA 
CGTAGCTCCA CGATGAGCAC CGAGAACACG GAGAATGGCC GACGTGACCA CTTGGCTAAT 
CTGCAACTAA GTCCTGTGCA AAAGCAAAAG CGAACGCCCG AAGTATCACC ACGCCGTTTC 
GGAGATCGCG ACAAGTCGGC CAAACGGAAA TCGAATTTGA ATCGTTCGTT GACCGAGGAA 
GCCACCAAGG ATGGCGATGA GGTTGCCTCT TCGACCCGCC GGCGCCGACG TCGTGAACTG 
GGAATGGCTC GTCCCCTGGT TCCGGGCGAT GATGACGCCC TAAGAGATTT GCTAACGCCG 
CAGAAGAGTT CCTGCAGCGA GTCAACCAAC TCCATATCCG AACCCCATCT CCAGTTCATA 
CCAGCCGTTC GTCATCCGGT GGTTCCAGTA CGCACCATGA CTGCTGTGCT GCCTGCACCA 
CGACCGCTGC ATACTCCTGC GGCCCGCAAC GTCAGCCAGA AGTTCAATGA AAGGACCAAA 
ACAGATCTGG CCGAGATCAA GAAGATTGCT GAGCAATCGG AGGCGCAGGT GCAACAGCCT 
TCGGTGGATG ATAACCAGAA AATGTCAGCC ATAATGCGCA GGAAGAGCAC GGATGAGGGT 
GGATCAGCTT TGGGGGCAAC AAACCAGAAT TCACCACCTG CCCCAAACCA AATTGTATCC 
ATACTGAAGA AGAAGGAACA TGGACTTGGC GAGTGCAACT CCAGTGCTTC AAGCAATCCC 
TCGCCGGTGA CCTTCTCTTC TAGCGTGGTG GATCATCCTT TGGCTGGAGC TGGCCGGTGC 
AAACGGCAGG GAATACTCAA GAAGAGATCG AGTCTAGATG AATCGCGCTA CTACTCCCGA 
TCGCATTCAC CCGACGAACG TAGTATTCTC ATTAAGTCGG CCAGGAGGAA TTCCCTGGAG 
GAAGCTGGCA CTGGATTGAG TCCGGCTCAG GCCCATGGAA TCCTCAAGCA GAGCAGCTAC 
GACAGCAGCA AGAGCGACGG CTGCCCTTCG GCCACGGAGA GCCAGCCACA CAGTATCCTG 
AAAAAGAAGG ATTCGCTATC GACACCTTCG GACGGAGGAT GCCACAAGCA TGTCTCTATA 
TCGCAAGCTG TAACTTTAGC TGCTGCTGAA CTGGCTGCCC ATGATGGAAT CACCTTCGAT 
GATGGTGAGG AGCATGAGAT ACGACCGATC CTAAAGCAGG AGAGCACCTC CAGCGAGGAG 
GCTGTGCGTC CGCCAAAGCC CATACTCAAA AAGAAATCCT TTGGCGAGGC GGATGAACAC 
GAAATACGTC CTATATTGAA GAGCTCTCGT AAAAGCAGTC GAGAGGAGTT CGATTTGAGT 
GGCTTGGAAA ACGAGGTCAA CGATTCCCTA TCGTCAATCC TAAAGACAGA CTCGCCGTCC 
AAGCGTCGGT CGCTCGGTTC AGCTTTGCAT GATTTGGAGG AGTCCACCAC TTCCCCGAGC 
ATGCTCAAGA GGCGCACCCG TTCGCTGGAA CGTCAAGATG TCCAGCCTGG CATGGATTTG 
GCAGCTGCAC TGGATGCCAT CGCCACATCA CAGGCTACTC CCAGCACCCC GGTGGACTTC 
GTGACCACAC CAACAAGTAT ATCGGTGGCG GAGCGGATCA AGCGCATGGA GATGCTGTCG 
ACGCCCACTT CCCGTGCGGC GGGTGGGGCC AACAGCAGCT GGGAATCGCC ACTGCAGGCA 
TCGTCAGCAG AGCATGGAGC ACCCACCACC CCAAGGGTTC TCAAGCCAAG TGTGCTGCGC 
AGAGATTTGC AGAGGGAGCG GTACAAAACG CAACCCGTGA CCAACGAGGA GATGAGCTTC 
TTCAAAGCCA ACTCCGCGCC CTTCGACATC TCGGCTACAT CGGCCTTCAA GCCCACCAAG 
CCGCTGGACA TCCGCTTGAC CCACGCACCG CATGCACCGC TGATTTCGCC GATCACCGGA 
CAGCCACTCA GCCACGTGCC CGCGCCACTG CTGCTATCCT CGTCGACGAA CTCGGGAGCG 
GGATCCTCGT TCCGTCTGGC CCGCAGCTCC ACCCAACCGC TGCAGTCGCC GCGAGTTGTC 
CACCTGGCCG GTCAGGCTAC TGGTGGCTCC GCTTTGGCAC GCCAGAACTC GGTGAACGAC 
AGCATCAATC TCAGCGAGCA GCTAACCCTA TCCATCGGCA CGGACAGCGA GCACATCTTT 
GAGATGTCCG CTTTGGAGGA GGACTCGGCT ATTCAGTCGC TGAGCGACGA GACAGCCGCT 
TCCGCATCTG CCAACTCAAC ATCCACGACC ACTGCAACCA CCATGACACC GCCCAGTGGC 
CTGACGCGGA GCAACAGCGT TCGGGCCAGG GCCAATATGT TCCAGCAATT GCAGGAGCAG 
TCGCGCAACC AACGGGCAAC GGGAACCACC AACTCCCGTC AATCTCCACC AGCGTCGTCG 
GCGGAGGTGT CTTCAAGTGC AGCCGACAAT TCCATGCACA GTGACGAGCG GTCCACGCCA 
AATGCAACGA TAGATGCGGA ATTCGAACCC TCATCCCTGT CGCTGGCTGA GCGACTGGCC 
TTCTTCAGCA ATCTGTGCGA GAGCGGCGGA GGAGGTGGGA GTACCAGTGC CGGTGGAAGG 
TACCGCAGTC GCACCAGCAG CTATTCAAGG AGTCCGCCCG GCGATCGCAC CACTCCCAGG 
AGCAGCACCA CCGCCAGCAG TGTCAGTATG ACGCCCACGC CCATGGATTA CTCTCCGATT 
CCCCACGACA AGGAGCCATC TAGTCTCACG CTGGAGAGCA TCATCGAGCC CGAGCAGGAG 
GAGGTCAAGG AGCAGGTGGA CCAGGATGAG GTCAAGTTGA GGAAGAAGGA GCCAGTCCAG 
AGGGAATCTT CCCAGCACAA TGGCTTCCAC GTGCTGACCA GGTCCGCCAC CGAACCGCCG 
TCGTCCACCT CGCCCCTGCG CATCAGCATA CGGACTGTGG GAAAGCTAGT CCTGCCCGAT 
ACCTTCCGCG GCGATCGCAA CAATAACAGC AGTAGTAGAA GTGGGAGCAA CAGCTGGAGC 
AACACTTGCA TGGTCAAGCT GCAGAGCTTG GAGCCCGTTA GCCTGTCCGT TCATTCGCGC 
ACCATTGGTA AGGTTAAGTC GCCATTCATT GAAAAGCAGC AGGAGAAACC ACACACCGAG 
AAGCTAGCGA ACGGAACCAG CGACAGTGGC TCCGATTCGG GCAAGGAGAA CTGCGCCTCC 
AACGAGCAGG AACATCTGCA GCACCAGCAG TCGCAGGACG AGGATCGAGC TCCGCCCAGG 
GTGTCCGAGA TGCGGAGGAA AATCAGCCGA CTGGTGCAAC AACAGCAGCC GCAACCACAG 
CCCGTCAATC GGCGGCACAC CACCGAGATC ACCTCCGTGA CCGTTGGAAC GCGTTCGCCC 
AGTGTGGACG ATCGCTTTGC CAAGTACTTT GGTGTTAAGG AGAGCTGCAA CAGCACGACC 
ACCGTGCATA AAACGCAATC GCTGCCACCC AGCCAAGTTG AGGCCACCAA TGCGCACGTG 
AAACTACCGC AGATCACTAC CGTGGTTTCC AAACGGCGCG AGCTGCTCAA GAGGACGCGA 
CCCCTGTCCA TGGATCACTA CTCCAGCGGC TGCACCAGTC CCACAGCCAT GCCAAGTCCC 
AAGAGAGCAC ATTCGCCCGT CGGCCGGCGG AAGGCCGCCA TTTCCAGCAT CGAGGACATT 
GAGGTCACGC CGGATGAACT CCGGGCCGCT GGCAGGAACT TCAAGCTGTT GTACGGCGAG 
TTATTGGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Svil ( NM_001202100.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001189029.1 Drosophila melanogaster supervillin, transcript variant N (Svil), mRNA.

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CloneID	OFa19995
Clone ID Related Accession (Same CDS sequence)	NM_001202100.2
Accession Version	NM_001202100.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4341bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	supervillin, isoform N
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001202100.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCAGCG GCAACTCGGG CGGCAGCAGT GGCAATCCCA AGCAGCAGCA GCAGCAGCAG 
CAGCAACAAC AGCTAGAGCA GCTGGGACAC CAGACACATC TTGTGCTAGG AGCATCAAAG 
GAGTTGCCCG CGGTCAGCAG CAGCAGCGAT GAAGAGGGAT CCCTGCGGAC TCTCCACTCC 
AGTCGCAAGG TGACCAATCT GAATGCCAAG TCCACCTCTC TGCCACCCGA GGAAGGACTC 
TCGGGTGATG TCTCCCTGGA GTCGTCCTCC AACCGACTGA ATCTGCGATT GGCCAGGAGC 
CTGGAGCCCT CAGTCCGCAA TATCACTGCA TCCGCCTCCA AGGAAGTACA GAGCTATGAT 
GCTGGGGAGC CTGTGAATAC CAATCGATTG TTTGTGGAAC CGCCCAAGAT ACCACTGTCT 
GCCATCCGAA ACTCTCTTGA CGATCACGAC CTGGATATTG AGGAGTGCGA CAGCACCACC 
CAGTTCGATC AGGGGGATAT CTACGATACC CTGGAACGCT GGAGCCATGG TGTCAGCTTG 
GGGGCACATC TATTGAACTG CGCGAATGGC CACGATCAAT CTGATCCCGA GGACGACCAT 
CAAACCGATC ATCATCAGCA TGCACATCAA TCATTGCCAG TAGAACCCAA GTCTCATCAT 
CATCAGCACC ACCAGGCCAC CAATGCGCGC AGATTCATAA CGCGCACCAC GCGTTCCGCC 
AGTCGCCAGA GTTCCGCCGG ACACTCCCCA CCTCCGCCGC TGCATCGCAG TGACGACTCC 
TCCAGCAGTT CACCCTCACC GACACGCAGA CGGAATAAGC CAGGTGGTGC TCCTGCCGCC 
ACAGATCCAC CATCATCGTC GGCGAACGGG AATAGTTCCG CTTATGAGAC GGCAGCCACC 
ACTGTGATGA CCCATGACCA GCAGCGAGAT CCTCCAGTTG AAGTTCCTAA GGCTCTAAAC 
GTCGAGCCAC CGCAGCCAGA AGACATCGTC CTGGGCTCCT GCCCCACTGC ATCTGCCCAC 
TCAGATAAAA AGAAGGAAGA GGAGCAACGC AATCGCTACA GTTCGCCCGT TTCACCAAAA 
GCCTCCAGCA ACGGCGGAGT CCAAGCACAA CGTCGTCTTA GCAACTCGGA GCTATCGCCC 
AAGTCGCCAG GTGCCAGTGG ATTCACTTCG GAGCCAGTCG CGTCAGCCAC AGCGAACAGA 
GTCCCGACGA GATCTGCCTC CCATCCTGTG GACACACCGC CCACACAAAG GCGACAGCCT 
CTGGTGGCCG TGAGTTCGGG CATCTCCATG AAGCAGAGTC TCAGCGAGCT GCACTTCACG 
GGTGGAGCAG TCAGTCCGCC CAAGGCTACG GCGGAATGCA CTCTAAGGAT GCGACCACGT 
AGCTCCACGA TGAGCACCGA GAACACGGAG AATGGCCGAC GTGACCACTT GGCTAATCTG 
CAACTAAGTC CTGTGCAAAA GCAAAAGCGA ACGCCCGAAG TATCACCACG CCGTTTCGGA 
GATCGCGACA AGTCGGCCAA ACGGAAATCG AATTTGAATC GTTCGTTGAC CGAGGAAGCC 
ACCAAGGATG GCGATGAGGT TGCCTCTTCG ACCCGCCGGC GCCGACGTCG TGAACTGGGA 
ATGGCTCGTC CCCTGGTTCC GGGCGATGAT GACGCCCTAA GAGATTTGCT AACGCCGCAG 
AAGAGTTCCT GCAGCGAGTC AACCAACTCC ATATCCGAAC CCCATCTCCA GTTCATACCA 
GCCGTTCGTC ATCCGGTGGT TCCAGTACGC ACCATGACTG CTGTGCTGCC TGCACCACGA 
CCGCTGCATA CTCCTGCGGC CCGCAACGTC AGCCAGAAGT TCAATGAAAG GACCAAAACA 
GATCTGGCCG AGATCAAGAA GATTGCTGAG CAATCGGAGG CGCAGGTGCA ACAGCCTTCG 
GTGGATGATA ACCAGAAAAT GTCAGCCATA ATGCGCAGGA AGAGCACGGA TGAGGGTGGA 
TCAGCTTTGG GGGCAACAAA CCAGAATTCA CCACCTGCCC CAAACCAAAT TGTATCCATA 
CTGAAGAAGA AGGAACATGG ACTTGGCGAG TGCAACTCCA GTGCTTCAAG CAATCCCTCG 
CCGGTGACCT TCTCTTCTAG CGTGGTGGAT CATCCTTTGG CTGGAGCTGG CCGGTGCAAA 
CGGCAGGGAA TACTCAAGAA GAGATCGAGT CTAGATGAAT CGCGCTACTA CTCCCGATCG 
CATTCACCCG ACGAACGTAG TATTCTCATT AAGTCGGCCA GGAGGAATTC CCTGGAGGAA 
GCTGGCACTG GATTGAGTCC GGCTCAGGCC CATGGAATCC TCAAGCAGAG CAGCTACGAC 
AGCAGCAAGA GCGACGGCTG CCCTTCGGCC ACGGAGAGCC AGCCACACAG TATCCTGAAA 
AAGAAGGATT CGCTATCGAC ACCTTCGGAC GGAGGATGCC ACAAGCATGT CTCTATATCG 
CAAGCTGTAA CTTTAGCTGC TGCTGAACTG GCTGCCCATG ATGGAATCAC CTTCGATGAT 
GGTGAGGAGC ATGAGATACG ACCGATCCTA AAGCAGGAGA GCACCTCCAG CGAGGAGGCT 
GTGCGTCCGC CAAAGCCCAT ACTCAAAAAG AAATCCTTTG GCGAGGCGGA TGAACACGAA 
ATACGTCCTA TATTGAAGAG CTCTCGTAAA AGCAGTCGAG AGGAGTTCGA TTTGAGTGGC 
TTGGAAAACG AGGTCAACGA TTCCCTATCG TCAATCCTAA AGACAGACTC GCCGTCCAAG 
CGTCGGTCGC TCGGTTCAGC TTTGCATGAT TTGGAGGAGT CCACCACTTC CCCGAGCATG 
CTCAAGAGGC GCACCCGTTC GCTGGAACGT CAAGATGTCC AGCCTGGCAT GGATTTGGCA 
GCTGCACTGG ATGCCATCGC CACATCACAG GCTACTCCCA GCACCCCGGT GGACTTCGTG 
ACCACACCAA CAAGTATATC GGTGGCGGAG CGGATCAAGC GCATGGAGAT GCTGTCGACG 
CCCACTTCCC GTGCGGCGGG TGGGGCCAAC AGCAGCTGGG AATCGCCACT GCAGGCATCG 
TCAGCAGAGC ATGGAGCACC CACCACCCCA AGGGTTCTCA AGCCAAGTGT GCTGCGCAGA 
GATTTGCAGA GGGAGCGGTA CAAAACGCAA CCCGTGACCA ACGAGGAGAT GAGCTTCTTC 
AAAGCCAACT CCGCGCCCTT CGACATCTCG GCTACATCGG CCTTCAAGCC CACCAAGCCG 
CTGGACATCC GCTTGACCCA CGCACCGCAT GCACCGCTGA TTTCGCCGAT CACCGGACAG 
CCACTCAGCC ACGTGCCCGC GCCACTGCTG CTATCCTCGT CGACGAACTC GGGAGCGGGA 
TCCTCGTTCC GTCTGGCCCG CAGCTCCACC CAACCGCTGC AGTCGCCGCG AGTTGTCCAC 
CTGGCCGGTC AGGCTACTGG TGGCTCCGCT TTGGCACGCC AGAACTCGGT GAACGACAGC 
ATCAATCTCA GCGAGCAGCT AACCCTATCC ATCGGCACGG ACAGCGAGCA CATCTTTGAG 
ATGTCCGCTT TGGAGGAGGA CTCGGCTATT CAGTCGCTGA GCGACGAGAC AGCCGCTTCC 
GCATCTGCCA ACTCAACATC CACGACCACT GCAACCACCA TGACACCGCC CAGTGGCCTG 
ACGCGGAGCA ACAGCGTTCG GGCCAGGGCC AATATGTTCC AGCAATTGCA GGAGCAGTCG 
CGCAACCAAC GGGCAACGGG AACCACCAAC TCCCGTCAAT CTCCACCAGC GTCGTCGGCG 
GAGGTGTCTT CAAGTGCAGC CGACAATTCC ATGCACAGTG ACGAGCGGTC CACGCCAAAT 
GCAACGATAG ATGCGGAATT CGGAAAACCC AGCACCGAAC CACTTAAGAG CATTCTTAAG 
ACGGGAATGC CAGTGATGGG TATGGGACGC GGTTTAATGC CTGTGGATCT CAATGCCGAG 
CTGAAGAACC GGCTCAAACG CTCCACACAT GCCACTGTTT CCAATCTCCG CAAATCTGCC 
ACCTCGGCAG ATGCCACTAG AGCAGCTAGT GATGAGGTGG ACAATCCGCA GAGGAATTTA 
GCCCAGATCC TGAGGAATGT TTCCAAGGAG AACTCCACGC CGAAGCACGA TGATGCCGTG 
AGCCTAGTGC GGAATCTCTC TACTTTGGGA CAACCTCGAT CGCTGGCCCG TGACGATGCA 
GCCGGGAATT GTGCCTCGGC ATCCGAAGGC GAGAGTTCGG GGGGCCGCGA GATCGAGGCC 
ATAATCAAGA ACAGCGCTGT GGCCAGACGT CGCCGGCAGC AACAGCAGCA GTCGCAGCAG 
CAGAATCAGC CAGATGGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189029.1
CDS	1216..5556
Translation	MSSGNSGGSSGNPKQQQQQQQQQQLEQLGHQTHLVLGASKELPAVSSSSDEEGSLRTLHSSRKVTNLNAKSTSLPPEEGLSGDVSLESSSNRLNLRLARSLEPSVRNITASASKEVQSYDAGEPVNTNRLFVEPPKIPLSAIRNSLDDHDLDIEECDSTTQFDQGDIYDTLERWSHGVSLGAHLLNCANGHDQSDPEDDHQTDHHQHAHQSLPVEPKSHHHQHHQATNARRFITRTTRSASRQSSAGHSPPPPLHRSDDSSSSSPSPTRRRNKPGGAPAATDPPSSSANGNSSAYETAATTVMTHDQQRDPPVEVPKALNVEPPQPEDIVLGSCPTASAHSDKKKEEEQRNRYSSPVSPKASSNGGVQAQRRLSNSELSPKSPGASGFTSEPVASATANRVPTRSASHPVDTPPTQRRQPLVAVSSGISMKQSLSELHFTGGAVSPPKATAECTLRMRPRSSTMSTENTENGRRDHLANLQLSPVQKQKRTPEVSPRRFGDRDKSAKRKSNLNRSLTEEATKDGDEVASSTRRRRRRELGMARPLVPGDDDALRDLLTPQKSSCSESTNSISEPHLQFIPAVRHPVVPVRTMTAVLPAPRPLHTPAARNVSQKFNERTKTDLAEIKKIAEQSEAQVQQPSVDDNQKMSAIMRRKSTDEGGSALGATNQNSPPAPNQIVSILKKKEHGLGECNSSASSNPSPVTFSSSVVDHPLAGAGRCKRQGILKKRSSLDESRYYSRSHSPDERSILIKSARRNSLEEAGTGLSPAQAHGILKQSSYDSSKSDGCPSATESQPHSILKKKDSLSTPSDGGCHKHVSISQAVTLAAAELAAHDGITFDDGEEHEIRPILKQESTSSEEAVRPPKPILKKKSFGEADEHEIRPILKSSRKSSREEFDLSGLENEVNDSLSSILKTDSPSKRRSLGSALHDLEESTTSPSMLKRRTRSLERQDVQPGMDLAAALDAIATSQATPSTPVDFVTTPTSISVAERIKRMEMLSTPTSRAAGGANSSWESPLQASSAEHGAPTTPRVLKPSVLRRDLQRERYKTQPVTNEEMSFFKANSAPFDISATSAFKPTKPLDIRLTHAPHAPLISPITGQPLSHVPAPLLLSSSTNSGAGSSFRLARSSTQPLQSPRVVHLAGQATGGSALARQNSVNDSINLSEQLTLSIGTDSEHIFEMSALEEDSAIQSLSDETAASASANSTSTTTATTMTPPSGLTRSNSVRARANMFQQLQEQSRNQRATGTTNSRQSPPASSAEVSSSAADNSMHSDERSTPNATIDAEFGKPSTEPLKSILKTGMPVMGMGRGLMPVDLNAELKNRLKRSTHATVSNLRKSATSADATRAASDEVDNPQRNLAQILRNVSKENSTPKHDDAVSLVRNLSTLGQPRSLARDDAAGNCASASEGESSGGREIEAIIKNSAVARRRRQQQQQSQQQNQPDG

Target ORF information:

RefSeq Version	NM_001202100.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster supervillin, transcript variant N (Svil), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202100.2

ORF Insert Sequence:

ATGAGCAGCG GCAACTCGGG CGGCAGCAGT GGCAATCCCA AGCAGCAGCA GCAGCAGCAG 
CAGCAACAAC AGCTAGAGCA GCTGGGACAC CAGACACATC TTGTGCTAGG AGCATCAAAG 
GAGTTGCCCG CGGTCAGCAG CAGCAGCGAT GAAGAGGGAT CCCTGCGGAC TCTCCACTCC 
AGTCGCAAGG TGACCAATCT GAATGCCAAG TCCACCTCTC TGCCACCCGA GGAAGGACTC 
TCGGGTGATG TCTCCCTGGA GTCGTCCTCC AACCGACTGA ATCTGCGATT GGCCAGGAGC 
CTGGAGCCCT CAGTCCGCAA TATCACTGCA TCCGCCTCCA AGGAAGTACA GAGCTATGAT 
GCTGGGGAGC CTGTGAATAC CAATCGATTG TTTGTGGAAC CGCCCAAGAT ACCACTGTCT 
GCCATCCGAA ACTCTCTTGA CGATCACGAC CTGGATATTG AGGAGTGCGA CAGCACCACC 
CAGTTCGATC AGGGGGATAT CTACGATACC CTGGAACGCT GGAGCCATGG TGTCAGCTTG 
GGGGCACATC TATTGAACTG CGCGAATGGC CACGATCAAT CTGATCCCGA GGACGACCAT 
CAAACCGATC ATCATCAGCA TGCACATCAA TCATTGCCAG TAGAACCCAA GTCTCATCAT 
CATCAGCACC ACCAGGCCAC CAATGCGCGC AGATTCATAA CGCGCACCAC GCGTTCCGCC 
AGTCGCCAGA GTTCCGCCGG ACACTCCCCA CCTCCGCCGC TGCATCGCAG TGACGACTCC 
TCCAGCAGTT CACCCTCACC GACACGCAGA CGGAATAAGC CAGGTGGTGC TCCTGCCGCC 
ACAGATCCAC CATCATCGTC GGCGAACGGG AATAGTTCCG CTTATGAGAC GGCAGCCACC 
ACTGTGATGA CCCATGACCA GCAGCGAGAT CCTCCAGTTG AAGTTCCTAA GGCTCTAAAC 
GTCGAGCCAC CGCAGCCAGA AGACATCGTC CTGGGCTCCT GCCCCACTGC ATCTGCCCAC 
TCAGATAAAA AGAAGGAAGA GGAGCAACGC AATCGCTACA GTTCGCCCGT TTCACCAAAA 
GCCTCCAGCA ACGGCGGAGT CCAAGCACAA CGTCGTCTTA GCAACTCGGA GCTATCGCCC 
AAGTCGCCAG GTGCCAGTGG ATTCACTTCG GAGCCAGTCG CGTCAGCCAC AGCGAACAGA 
GTCCCGACGA GATCTGCCTC CCATCCTGTG GACACACCGC CCACACAAAG GCGACAGCCT 
CTGGTGGCCG TGAGTTCGGG CATCTCCATG AAGCAGAGTC TCAGCGAGCT GCACTTCACG 
GGTGGAGCAG TCAGTCCGCC CAAGGCTACG GCGGAATGCA CTCTAAGGAT GCGACCACGT 
AGCTCCACGA TGAGCACCGA GAACACGGAG AATGGCCGAC GTGACCACTT GGCTAATCTG 
CAACTAAGTC CTGTGCAAAA GCAAAAGCGA ACGCCCGAAG TATCACCACG CCGTTTCGGA 
GATCGCGACA AGTCGGCCAA ACGGAAATCG AATTTGAATC GTTCGTTGAC CGAGGAAGCC 
ACCAAGGATG GCGATGAGGT TGCCTCTTCG ACCCGCCGGC GCCGACGTCG TGAACTGGGA 
ATGGCTCGTC CCCTGGTTCC GGGCGATGAT GACGCCCTAA GAGATTTGCT AACGCCGCAG 
AAGAGTTCCT GCAGCGAGTC AACCAACTCC ATATCCGAAC CCCATCTCCA GTTCATACCA 
GCCGTTCGTC ATCCGGTGGT TCCAGTACGC ACCATGACTG CTGTGCTGCC TGCACCACGA 
CCGCTGCATA CTCCTGCGGC CCGCAACGTC AGCCAGAAGT TCAATGAAAG GACCAAAACA 
GATCTGGCCG AGATCAAGAA GATTGCTGAG CAATCGGAGG CGCAGGTGCA ACAGCCTTCG 
GTGGATGATA ACCAGAAAAT GTCAGCCATA ATGCGCAGGA AGAGCACGGA TGAGGGTGGA 
TCAGCTTTGG GGGCAACAAA CCAGAATTCA CCACCTGCCC CAAACCAAAT TGTATCCATA 
CTGAAGAAGA AGGAACATGG ACTTGGCGAG TGCAACTCCA GTGCTTCAAG CAATCCCTCG 
CCGGTGACCT TCTCTTCTAG CGTGGTGGAT CATCCTTTGG CTGGAGCTGG CCGGTGCAAA 
CGGCAGGGAA TACTCAAGAA GAGATCGAGT CTAGATGAAT CGCGCTACTA CTCCCGATCG 
CATTCACCCG ACGAACGTAG TATTCTCATT AAGTCGGCCA GGAGGAATTC CCTGGAGGAA 
GCTGGCACTG GATTGAGTCC GGCTCAGGCC CATGGAATCC TCAAGCAGAG CAGCTACGAC 
AGCAGCAAGA GCGACGGCTG CCCTTCGGCC ACGGAGAGCC AGCCACACAG TATCCTGAAA 
AAGAAGGATT CGCTATCGAC ACCTTCGGAC GGAGGATGCC ACAAGCATGT CTCTATATCG 
CAAGCTGTAA CTTTAGCTGC TGCTGAACTG GCTGCCCATG ATGGAATCAC CTTCGATGAT 
GGTGAGGAGC ATGAGATACG ACCGATCCTA AAGCAGGAGA GCACCTCCAG CGAGGAGGCT 
GTGCGTCCGC CAAAGCCCAT ACTCAAAAAG AAATCCTTTG GCGAGGCGGA TGAACACGAA 
ATACGTCCTA TATTGAAGAG CTCTCGTAAA AGCAGTCGAG AGGAGTTCGA TTTGAGTGGC 
TTGGAAAACG AGGTCAACGA TTCCCTATCG TCAATCCTAA AGACAGACTC GCCGTCCAAG 
CGTCGGTCGC TCGGTTCAGC TTTGCATGAT TTGGAGGAGT CCACCACTTC CCCGAGCATG 
CTCAAGAGGC GCACCCGTTC GCTGGAACGT CAAGATGTCC AGCCTGGCAT GGATTTGGCA 
GCTGCACTGG ATGCCATCGC CACATCACAG GCTACTCCCA GCACCCCGGT GGACTTCGTG 
ACCACACCAA CAAGTATATC GGTGGCGGAG CGGATCAAGC GCATGGAGAT GCTGTCGACG 
CCCACTTCCC GTGCGGCGGG TGGGGCCAAC AGCAGCTGGG AATCGCCACT GCAGGCATCG 
TCAGCAGAGC ATGGAGCACC CACCACCCCA AGGGTTCTCA AGCCAAGTGT GCTGCGCAGA 
GATTTGCAGA GGGAGCGGTA CAAAACGCAA CCCGTGACCA ACGAGGAGAT GAGCTTCTTC 
AAAGCCAACT CCGCGCCCTT CGACATCTCG GCTACATCGG CCTTCAAGCC CACCAAGCCG 
CTGGACATCC GCTTGACCCA CGCACCGCAT GCACCGCTGA TTTCGCCGAT CACCGGACAG 
CCACTCAGCC ACGTGCCCGC GCCACTGCTG CTATCCTCGT CGACGAACTC GGGAGCGGGA 
TCCTCGTTCC GTCTGGCCCG CAGCTCCACC CAACCGCTGC AGTCGCCGCG AGTTGTCCAC 
CTGGCCGGTC AGGCTACTGG TGGCTCCGCT TTGGCACGCC AGAACTCGGT GAACGACAGC 
ATCAATCTCA GCGAGCAGCT AACCCTATCC ATCGGCACGG ACAGCGAGCA CATCTTTGAG 
ATGTCCGCTT TGGAGGAGGA CTCGGCTATT CAGTCGCTGA GCGACGAGAC AGCCGCTTCC 
GCATCTGCCA ACTCAACATC CACGACCACT GCAACCACCA TGACACCGCC CAGTGGCCTG 
ACGCGGAGCA ACAGCGTTCG GGCCAGGGCC AATATGTTCC AGCAATTGCA GGAGCAGTCG 
CGCAACCAAC GGGCAACGGG AACCACCAAC TCCCGTCAAT CTCCACCAGC GTCGTCGGCG 
GAGGTGTCTT CAAGTGCAGC CGACAATTCC ATGCACAGTG ACGAGCGGTC CACGCCAAAT 
GCAACGATAG ATGCGGAATT CGGAAAACCC AGCACCGAAC CACTTAAGAG CATTCTTAAG 
ACGGGAATGC CAGTGATGGG TATGGGACGC GGTTTAATGC CTGTGGATCT CAATGCCGAG 
CTGAAGAACC GGCTCAAACG CTCCACACAT GCCACTGTTT CCAATCTCCG CAAATCTGCC 
ACCTCGGCAG ATGCCACTAG AGCAGCTAGT GATGAGGTGG ACAATCCGCA GAGGAATTTA 
GCCCAGATCC TGAGGAATGT TTCCAAGGAG AACTCCACGC CGAAGCACGA TGATGCCGTG 
AGCCTAGTGC GGAATCTCTC TACTTTGGGA CAACCTCGAT CGCTGGCCCG TGACGATGCA 
GCCGGGAATT GTGCCTCGGC ATCCGAAGGC GAGAGTTCGG GGGGCCGCGA GATCGAGGCC 
ATAATCAAGA ACAGCGCTGT GGCCAGACGT CGCCGGCAGC AACAGCAGCA GTCGCAGCAG 
CAGAATCAGC CAGATGGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Svil ( NM_001259638.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246567.1 Drosophila melanogaster supervillin, transcript variant R (Svil), mRNA.

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CloneID	OFa19997
Clone ID Related Accession (Same CDS sequence)	NM_001259638.1 , NM_001169858.2
Accession Version	NM_001259638.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	supervillin, isoform R
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCAGCG GCAACTCGGG CGGCAGCAGT GGCAATCCCA AGCAGCAGCA GCAGCAGCAG 
CAGCAACAAC AGCTAGAGCA GCTGGGACAC CAGACACATC TTGTGCTAGG AGCATCAAAG 
GAGTTGCCCG CGGTCAGCAG CAGCAGCGAT GAAGAGGGAT CCCTGCGGAC TCTCCACTCC 
AGTCGCAAGG TGACCAATCT GAATGCCAAG TCCACCTCTC TGCCACCCGA GGAAGGACTC 
TCGGGTGATG TCTCCCTGGA GTCGTCCTCC AACCGACTGA ATCTGCGATT GGCCAGGAGC 
CTGGAGCCCT CAGTCCGCAA TATCACTGCA TCCGCCTCCA AGGAAGTACA GAGCTATGAT 
GCTGGGGAGC CTGTGAATAC CAATCGATTG TTTGTGGAAC CGCCCAAGAT ACCACTGTCT 
GCCATCCGAA ACTCTCTTGA CGATCACGAC CTGGATATTG AGGAGTGCGA CAGCACCACC 
CAGTTCGATC AGGGGGATAT CTACGATACC CTGGAACGCT GGAGCCATGG TGTCAGCTTG 
GGGGCACATC TATTGAACTG CGCGAATGGC CACGATCAAT CTGATCCCGA GGACGACCAT 
CAAACCGATC ATCATCAGCA TGCACATCAA TCATTGCCAG TAGAACCCAA GTCTCATCAT 
CATCAGCACC ACCAGGCCAC CAATGCGCGC AGATTCATAA CGCGCACCAC GCGTTCCGCC 
AGTCGCCAGA GTTCCGCCGG ACACTCCCCA CCTCCGCCGC TGCATCGCAG TGACGACTCC 
TCCAGCAGTT CACCCTCACC GACACGCAGA CGGAATAAGC CAGGTGGTGC TCCTGCCGCC 
ACAGATCCAC CATCATCGTC GGCGAACGGG AATAGTTCCG CTTATGAGAC GGCAGCCACC 
ACTGTGATGA CCCATGACCA GCAGCGAGAT CCTCCAGTTG AAGTTCCTAA GGCTCTAAAC 
GTCGAGCCAC CGCAGCCAGA AGACATCGTC CTGGGCTCCT GCCCCACTGC ATCTGCCCAC 
TCAGATAAAA AGAAGGAAGA GGAGCAACGC AATCGCTACA GTTCGCCCGT TTCACCAAAA 
GCCTCCAGCA ACGGCGGAGT CCAAGCACAA CGTCGTCTTA GCAACTCGGA GCTATCGCCC 
AAGTCGCCAG GTGCCAGTGG ATTCACTTCG GAGCCAGTCG CGTCAGCCAC AGCGAACAGA 
GTCCCGACGA GATCTGCCTC CCATCCTGTG GACACACCGC CCACACAAAG GCGACAGCCT 
CTGGTGGCCG TGAGTTCGGG CATCTCCATG AAGCAGAGTC TCAGCGAGCT GCACTTCACG 
GGTGGAGCAG TCAGTCCGCC CAAGGCTACG GCGGAATGCA CTCTAAGGAT GCGACCACGT 
AGCTCCACGA TGAGCACCGA GAACACGGAG AATGGCCGAC GTGACCACTT GGCTAATCTG 
CAACTAAGTC CTGTGCAAAA GCAAAAGCGA ACGCCCGAAG TATCACCACG CCGTTTCGGA 
GATCGCGACA AGTCGGCCAA ACGGAAATCG AATTTGAATC GTTCGTTGAC CGAGGAAGCC 
ACCAAGGATG GCGATGAGGT TGCCTCTTCG ACCCGCCGGC GCCGACGTCG TGAACTGGGA 
ATGGCTCGTC CCCTGGTTCC GGGCGATGAT GACGCCCTAA GAGATTTGCT AACGCCGCAG 
AAGAGTTCCT GCAGCGAGTC AACCAACTCC ATATCCGAAC CCCATCTCCA GTTCATACCA 
GCCGTTCGTC ATCCGGTGGT TCCAGTACGC ACCATGACTG CTGTGCTGCC TGCACCACGA 
CCGCTGCATA CTCCTGCGGC CCGCAACGTC AGCCAGAAGT TCAATGAAAG GACCAAAACA 
GATCTGGCCG AGATCAAGAA GATTGCTGAG CAATCGGAGG CGCAGGTGCA ACAGCCTTCG 
GTGGATGATA ACCAGAAAAT GTCAGCCATA ATGCGCAGGA AGAGCACGGA TGAGGGTGGA 
TCAGCTTTGG GGGCAACAAA CCAGAATTCA CCACCTGCCC CAAACCAAAT TGTATCCATA 
CTGAAGAAGA AGGAACATGG ACTTGGCGAG TGCAACTCCA GTGCTTCAAG CAATCCCTCG 
CCGGTGACCT TCTCTTCTAG CGTGGTGGAT CATCCTTTGG CTGGAGCTGG CCGGTGCAAA 
CGGCAGGGAA TACTCAAGAA GAGATCGAGT CTAGATGAAT CGCGCTACTA CTCCCGATCG 
CATTCACCCG ACGAACGTAG TATTCTCATT AAGTCGGCCA GGAGGAATTC CCTGGAGGAA 
GCTGGCACTG GATTGAGTCC GGCTCAGGCC CATGGAATCC TCAAGCAGAG CAGCTACGAC 
AGCAGCAAGA GCGACGGCTG CCCTTCGGCC ACGGAGAGCC AGCCACACAG TATCCTGAAA 
AAGAAGGATT CGCTATCGAC ACCTTCGGAC GGAGGATGCC ACAAGCATGT CTCTATATCG 
CAAGCTGTAA CTTTAGCTGC TGCTGAACTG GCTGCCCATG ATGGAATCAC CTTCGATGAT 
GGTGAGGAGC ATGAGATACG ACCGATCCTA AAGCAGGAGA GCACCTCCAG CGAGGAGGCT 
GTGCGTCCGC CAAAGCCCAT ACTCAAAAAG AAATCCTTTG GCGAGGCGGA TGAACACGAA 
ATACGTCCTA TATTGAAGAG CTCTCGTAAA AGCAGTCGAG AGGAGTTCGA TTTGAGTGGC 
TTGGAAAACG AGGTCAACGA TTCCCTATCG TCAATCCTAA AGACAGACTC GCCGTCCAAG 
CGTCGGTCGC TCGGTTCAGC TTTGCATGAT TTGGAGGAGT CCACCACTTC CCCGAGCATG 
CTCAAGAGGC GCACCCGTTC GCTGGAACGT CAAGATGTCC AGCCTGGCAT GGATTTGGCA 
GCTGCACTGG ATGCCATCGC CACATCACAG GCTACTCCCA GCACCCCGGT GGACTTCGTG 
ACCACACCAA CAAGTATATC GGTGGCGGAG CGGATCAAGC GCATGGAGAT GCTGTCGACG 
CCCACTTCCC GTGCGGCGGG TGGGGCCAAC AGCAGCTGGG AATCGCCACT GCAGGCATCG 
TCAGCAGAGC ATGGAGCACC CACCACCCCA AGGGTTCTCA AGCCAAGTGT GCTGCGCAGA 
GATTTGCAGA GGGAGCGGTA CAAAACGCAA CCCGTGACCA ACGAGGAGAT GAGCTTCTTC 
AAAGCCAACT CCGCGCCCTT CGACATCTCG GCTACATCGG CCTTCAAGCC CACCAAGCCG 
CTGGACATCC GCTTGACCCA CGCACCGCAT GCACCGCTGA TTTCGCCGAT CACCGGACAG 
CCACTCAGCC ACGTGCCCGC GCCACTGCTG CTATCCTCGT CGACGAACTC GGGAGCGGGA 
TCCTCGTTCC GTCTGGCCCG CAGCTCCACC CAACCGCTGC AGTCGCCGCG AGTTGTCCAC 
CTGGCCGGTC AGGCTACTGG TGGCTCCGCT TTGGCACGCC AGAACTCGGT GAACGACAGC 
ATCAATCTCA GCGAGCAGCT AACCCTATCC ATCGGCACGG ACAGCGAGCA CATCTTTGAG 
ATGTCCGCTT TGGAGGAGGA CTCGGCTATT CAGTCGCTGA GCGACGAGAC AGCCGCTTCC 
GCATCTGCCA ACTCAACATC CACGACCACT GCAACCACCA TGACACCGCC CAGTGGCCTG 
ACGCGGAGCA ACAGCGTTCG GGCCAGGGCC AATATGTTCC AGCAATTGCA GGAGCAGTCG 
CGCAACCAAC GGGCAACGGG AACCACCAAC TCCCGTCAAT CTCCACCAGC GTCGTCGGCG 
GAGGTGTCTT CAAGTGCAGC CGACAATTCC ATGCACAGTG ACGAGCGGTC CACGCCAAAT 
GCAACGATAG ATGCGGAATT CGAACCCTCA TCCCTGTCGC TGGCTGAGCG ACTGGCCTTC 
TTCAGCAATC TGTGCGAGAG CGGCGGAGGA GGTGGGAGTA CCAGTGCCGG TGGAAGGTAC 
CGCAGTCGCA CCAGCAGCTA TTCAAGGAGT CCGCCCGGCG ATCGCACCAC TCCCAGGAGC 
AGCACCACCG CCAGCAGTGT CAGTATGACG CCCACGCCCA TGGATTACTC TCCGATTCCC 
CACGACAAGG AGCCATCTAG TCTCACGCTG GAGAGCATCA TCGAGCCCGA GCAGGAGGAG 
GTCAAGGAGC AGGTGGACCA GGATGAGGTC AAGTTGAGGA AGAAGGAGCC AGTCCAGAGG 
GAATCTTCCC AGCACAATGG CTTCCACGTG CTGACCAGGT CCGCCACCGA ACCGCCGTCG 
TCCACCTCGC CCCTGCGCAT CAGCATACGG ACTGTGGGAA AGCTAGTCCT GCCCGATACC 
TTCCGCGGCG ATCGCAACAA TAACAGCAGT AGTAGAAGTG GGAGCAACAG CTGGAGCAAC 
ACTTGCATGG TCAAGCTGCA GAGCTTGGAG CCCGTTAGCC TGTCCGTTCA TTCGCGCACC 
ATTGGTAAGG TTAAGTCGCC ATTCATTGAA AAGCAGCAGG AGAAACCACA CACCGAGAAG 
CTAGCGAACG GAACCAGCGA CAGTGGCTCC GATTCGGGCA AGGAGAACTG CGCCTCCAAC 
GAGCAGGAAC ATCTGCAGCA CCAGCAGTCG CAGGACGAGG ATCGAGCTCC GCCCAGGGTG 
TCCGAGATGC GGAGGAAAAT CAGCCGACTG GTGCAACAAC AGCAGCCGCA ACCACAGCCC 
GTCAATCGGC GGCACACCAC CGAGATCACC TCCGTGACCG TTGGAACGCG TTCGCCCAGT 
GTGGACGATC GCTTTGCCAA GTACTTTGGT GTTAAGGAGA GCTGCAACAG CACGACCACC 
GTGCATAAAA CGCAATCGCT GCCACCCAGC CAAGTTGAGG CCACCAATGC GCACGTGAAA 
CTACCGCAGA TCACTACCGT GGTTTCCAAA CGGCGCGAGC TGCTCAAGAG GACGCGACCC 
CTGTCCATGG ATCACTACTC CAGCGGCTGC ACCAGTCCCA CAGCCATGCC AAGTCCCAAG 
AGAGCACATT CGCCCGTCGG CCGGCGGAAG GCCGCCATTT CCAGCATCGA GGACATTGAG 
GTCACGCCGG ATGAACTCCG GGCCGCTGGC AGGAACTTCA AGCTGTTGTA CGGCGAGTTA 
TTGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246567.1
CDS	1293..6401
Translation	MSSGNSGGSSGNPKQQQQQQQQQQLEQLGHQTHLVLGASKELPAVSSSSDEEGSLRTLHSSRKVTNLNAKSTSLPPEEGLSGDVSLESSSNRLNLRLARSLEPSVRNITASASKEVQSYDAGEPVNTNRLFVEPPKIPLSAIRNSLDDHDLDIEECDSTTQFDQGDIYDTLERWSHGVSLGAHLLNCANGHDQSDPEDDHQTDHHQHAHQSLPVEPKSHHHQHHQATNARRFITRTTRSASRQSSAGHSPPPPLHRSDDSSSSSPSPTRRRNKPGGAPAATDPPSSSANGNSSAYETAATTVMTHDQQRDPPVEVPKALNVEPPQPEDIVLGSCPTASAHSDKKKEEEQRNRYSSPVSPKASSNGGVQAQRRLSNSELSPKSPGASGFTSEPVASATANRVPTRSASHPVDTPPTQRRQPLVAVSSGISMKQSLSELHFTGGAVSPPKATAECTLRMRPRSSTMSTENTENGRRDHLANLQLSPVQKQKRTPEVSPRRFGDRDKSAKRKSNLNRSLTEEATKDGDEVASSTRRRRRRELGMARPLVPGDDDALRDLLTPQKSSCSESTNSISEPHLQFIPAVRHPVVPVRTMTAVLPAPRPLHTPAARNVSQKFNERTKTDLAEIKKIAEQSEAQVQQPSVDDNQKMSAIMRRKSTDEGGSALGATNQNSPPAPNQIVSILKKKEHGLGECNSSASSNPSPVTFSSSVVDHPLAGAGRCKRQGILKKRSSLDESRYYSRSHSPDERSILIKSARRNSLEEAGTGLSPAQAHGILKQSSYDSSKSDGCPSATESQPHSILKKKDSLSTPSDGGCHKHVSISQAVTLAAAELAAHDGITFDDGEEHEIRPILKQESTSSEEAVRPPKPILKKKSFGEADEHEIRPILKSSRKSSREEFDLSGLENEVNDSLSSILKTDSPSKRRSLGSALHDLEESTTSPSMLKRRTRSLERQDVQPGMDLAAALDAIATSQATPSTPVDFVTTPTSISVAERIKRMEMLSTPTSRAAGGANSSWESPLQASSAEHGAPTTPRVLKPSVLRRDLQRERYKTQPVTNEEMSFFKANSAPFDISATSAFKPTKPLDIRLTHAPHAPLISPITGQPLSHVPAPLLLSSSTNSGAGSSFRLARSSTQPLQSPRVVHLAGQATGGSALARQNSVNDSINLSEQLTLSIGTDSEHIFEMSALEEDSAIQSLSDETAASASANSTSTTTATTMTPPSGLTRSNSVRARANMFQQLQEQSRNQRATGTTNSRQSPPASSAEVSSSAADNSMHSDERSTPNATIDAEFEPSSLSLAERLAFFSNLCESGGGGGSTSAGGRYRSRTSSYSRSPPGDRTTPRSSTTASSVSMTPTPMDYSPIPHDKEPSSLTLESIIEPEQEEVKEQVDQDEVKLRKKEPVQRESSQHNGFHVLTRSATEPPSSTSPLRISIRTVGKLVLPDTFRGDRNNNSSSRSGSNSWSNTCMVKLQSLEPVSLSVHSRTIGKVKSPFIEKQQEKPHTEKLANGTSDSGSDSGKENCASNEQEHLQHQQSQDEDRAPPRVSEMRRKISRLVQQQQPQPQPVNRRHTTEITSVTVGTRSPSVDDRFAKYFGVKESCNSTTTVHKTQSLPPSQVEATNAHVKLPQITTVVSKRRELLKRTRPLSMDHYSSGCTSPTAMPSPKRAHSPVGRRKAAISSIEDIEVTPDELRAAGRNFKLLYGELLG

Target ORF information:

RefSeq Version	NM_001259638.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster supervillin, transcript variant R (Svil), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259638.1

ORF Insert Sequence:

ATGAGCAGCG GCAACTCGGG CGGCAGCAGT GGCAATCCCA AGCAGCAGCA GCAGCAGCAG 
CAGCAACAAC AGCTAGAGCA GCTGGGACAC CAGACACATC TTGTGCTAGG AGCATCAAAG 
GAGTTGCCCG CGGTCAGCAG CAGCAGCGAT GAAGAGGGAT CCCTGCGGAC TCTCCACTCC 
AGTCGCAAGG TGACCAATCT GAATGCCAAG TCCACCTCTC TGCCACCCGA GGAAGGACTC 
TCGGGTGATG TCTCCCTGGA GTCGTCCTCC AACCGACTGA ATCTGCGATT GGCCAGGAGC 
CTGGAGCCCT CAGTCCGCAA TATCACTGCA TCCGCCTCCA AGGAAGTACA GAGCTATGAT 
GCTGGGGAGC CTGTGAATAC CAATCGATTG TTTGTGGAAC CGCCCAAGAT ACCACTGTCT 
GCCATCCGAA ACTCTCTTGA CGATCACGAC CTGGATATTG AGGAGTGCGA CAGCACCACC 
CAGTTCGATC AGGGGGATAT CTACGATACC CTGGAACGCT GGAGCCATGG TGTCAGCTTG 
GGGGCACATC TATTGAACTG CGCGAATGGC CACGATCAAT CTGATCCCGA GGACGACCAT 
CAAACCGATC ATCATCAGCA TGCACATCAA TCATTGCCAG TAGAACCCAA GTCTCATCAT 
CATCAGCACC ACCAGGCCAC CAATGCGCGC AGATTCATAA CGCGCACCAC GCGTTCCGCC 
AGTCGCCAGA GTTCCGCCGG ACACTCCCCA CCTCCGCCGC TGCATCGCAG TGACGACTCC 
TCCAGCAGTT CACCCTCACC GACACGCAGA CGGAATAAGC CAGGTGGTGC TCCTGCCGCC 
ACAGATCCAC CATCATCGTC GGCGAACGGG AATAGTTCCG CTTATGAGAC GGCAGCCACC 
ACTGTGATGA CCCATGACCA GCAGCGAGAT CCTCCAGTTG AAGTTCCTAA GGCTCTAAAC 
GTCGAGCCAC CGCAGCCAGA AGACATCGTC CTGGGCTCCT GCCCCACTGC ATCTGCCCAC 
TCAGATAAAA AGAAGGAAGA GGAGCAACGC AATCGCTACA GTTCGCCCGT TTCACCAAAA 
GCCTCCAGCA ACGGCGGAGT CCAAGCACAA CGTCGTCTTA GCAACTCGGA GCTATCGCCC 
AAGTCGCCAG GTGCCAGTGG ATTCACTTCG GAGCCAGTCG CGTCAGCCAC AGCGAACAGA 
GTCCCGACGA GATCTGCCTC CCATCCTGTG GACACACCGC CCACACAAAG GCGACAGCCT 
CTGGTGGCCG TGAGTTCGGG CATCTCCATG AAGCAGAGTC TCAGCGAGCT GCACTTCACG 
GGTGGAGCAG TCAGTCCGCC CAAGGCTACG GCGGAATGCA CTCTAAGGAT GCGACCACGT 
AGCTCCACGA TGAGCACCGA GAACACGGAG AATGGCCGAC GTGACCACTT GGCTAATCTG 
CAACTAAGTC CTGTGCAAAA GCAAAAGCGA ACGCCCGAAG TATCACCACG CCGTTTCGGA 
GATCGCGACA AGTCGGCCAA ACGGAAATCG AATTTGAATC GTTCGTTGAC CGAGGAAGCC 
ACCAAGGATG GCGATGAGGT TGCCTCTTCG ACCCGCCGGC GCCGACGTCG TGAACTGGGA 
ATGGCTCGTC CCCTGGTTCC GGGCGATGAT GACGCCCTAA GAGATTTGCT AACGCCGCAG 
AAGAGTTCCT GCAGCGAGTC AACCAACTCC ATATCCGAAC CCCATCTCCA GTTCATACCA 
GCCGTTCGTC ATCCGGTGGT TCCAGTACGC ACCATGACTG CTGTGCTGCC TGCACCACGA 
CCGCTGCATA CTCCTGCGGC CCGCAACGTC AGCCAGAAGT TCAATGAAAG GACCAAAACA 
GATCTGGCCG AGATCAAGAA GATTGCTGAG CAATCGGAGG CGCAGGTGCA ACAGCCTTCG 
GTGGATGATA ACCAGAAAAT GTCAGCCATA ATGCGCAGGA AGAGCACGGA TGAGGGTGGA 
TCAGCTTTGG GGGCAACAAA CCAGAATTCA CCACCTGCCC CAAACCAAAT TGTATCCATA 
CTGAAGAAGA AGGAACATGG ACTTGGCGAG TGCAACTCCA GTGCTTCAAG CAATCCCTCG 
CCGGTGACCT TCTCTTCTAG CGTGGTGGAT CATCCTTTGG CTGGAGCTGG CCGGTGCAAA 
CGGCAGGGAA TACTCAAGAA GAGATCGAGT CTAGATGAAT CGCGCTACTA CTCCCGATCG 
CATTCACCCG ACGAACGTAG TATTCTCATT AAGTCGGCCA GGAGGAATTC CCTGGAGGAA 
GCTGGCACTG GATTGAGTCC GGCTCAGGCC CATGGAATCC TCAAGCAGAG CAGCTACGAC 
AGCAGCAAGA GCGACGGCTG CCCTTCGGCC ACGGAGAGCC AGCCACACAG TATCCTGAAA 
AAGAAGGATT CGCTATCGAC ACCTTCGGAC GGAGGATGCC ACAAGCATGT CTCTATATCG 
CAAGCTGTAA CTTTAGCTGC TGCTGAACTG GCTGCCCATG ATGGAATCAC CTTCGATGAT 
GGTGAGGAGC ATGAGATACG ACCGATCCTA AAGCAGGAGA GCACCTCCAG CGAGGAGGCT 
GTGCGTCCGC CAAAGCCCAT ACTCAAAAAG AAATCCTTTG GCGAGGCGGA TGAACACGAA 
ATACGTCCTA TATTGAAGAG CTCTCGTAAA AGCAGTCGAG AGGAGTTCGA TTTGAGTGGC 
TTGGAAAACG AGGTCAACGA TTCCCTATCG TCAATCCTAA AGACAGACTC GCCGTCCAAG 
CGTCGGTCGC TCGGTTCAGC TTTGCATGAT TTGGAGGAGT CCACCACTTC CCCGAGCATG 
CTCAAGAGGC GCACCCGTTC GCTGGAACGT CAAGATGTCC AGCCTGGCAT GGATTTGGCA 
GCTGCACTGG ATGCCATCGC CACATCACAG GCTACTCCCA GCACCCCGGT GGACTTCGTG 
ACCACACCAA CAAGTATATC GGTGGCGGAG CGGATCAAGC GCATGGAGAT GCTGTCGACG 
CCCACTTCCC GTGCGGCGGG TGGGGCCAAC AGCAGCTGGG AATCGCCACT GCAGGCATCG 
TCAGCAGAGC ATGGAGCACC CACCACCCCA AGGGTTCTCA AGCCAAGTGT GCTGCGCAGA 
GATTTGCAGA GGGAGCGGTA CAAAACGCAA CCCGTGACCA ACGAGGAGAT GAGCTTCTTC 
AAAGCCAACT CCGCGCCCTT CGACATCTCG GCTACATCGG CCTTCAAGCC CACCAAGCCG 
CTGGACATCC GCTTGACCCA CGCACCGCAT GCACCGCTGA TTTCGCCGAT CACCGGACAG 
CCACTCAGCC ACGTGCCCGC GCCACTGCTG CTATCCTCGT CGACGAACTC GGGAGCGGGA 
TCCTCGTTCC GTCTGGCCCG CAGCTCCACC CAACCGCTGC AGTCGCCGCG AGTTGTCCAC 
CTGGCCGGTC AGGCTACTGG TGGCTCCGCT TTGGCACGCC AGAACTCGGT GAACGACAGC 
ATCAATCTCA GCGAGCAGCT AACCCTATCC ATCGGCACGG ACAGCGAGCA CATCTTTGAG 
ATGTCCGCTT TGGAGGAGGA CTCGGCTATT CAGTCGCTGA GCGACGAGAC AGCCGCTTCC 
GCATCTGCCA ACTCAACATC CACGACCACT GCAACCACCA TGACACCGCC CAGTGGCCTG 
ACGCGGAGCA ACAGCGTTCG GGCCAGGGCC AATATGTTCC AGCAATTGCA GGAGCAGTCG 
CGCAACCAAC GGGCAACGGG AACCACCAAC TCCCGTCAAT CTCCACCAGC GTCGTCGGCG 
GAGGTGTCTT CAAGTGCAGC CGACAATTCC ATGCACAGTG ACGAGCGGTC CACGCCAAAT 
GCAACGATAG ATGCGGAATT CGAACCCTCA TCCCTGTCGC TGGCTGAGCG ACTGGCCTTC 
TTCAGCAATC TGTGCGAGAG CGGCGGAGGA GGTGGGAGTA CCAGTGCCGG TGGAAGGTAC 
CGCAGTCGCA CCAGCAGCTA TTCAAGGAGT CCGCCCGGCG ATCGCACCAC TCCCAGGAGC 
AGCACCACCG CCAGCAGTGT CAGTATGACG CCCACGCCCA TGGATTACTC TCCGATTCCC 
CACGACAAGG AGCCATCTAG TCTCACGCTG GAGAGCATCA TCGAGCCCGA GCAGGAGGAG 
GTCAAGGAGC AGGTGGACCA GGATGAGGTC AAGTTGAGGA AGAAGGAGCC AGTCCAGAGG 
GAATCTTCCC AGCACAATGG CTTCCACGTG CTGACCAGGT CCGCCACCGA ACCGCCGTCG 
TCCACCTCGC CCCTGCGCAT CAGCATACGG ACTGTGGGAA AGCTAGTCCT GCCCGATACC 
TTCCGCGGCG ATCGCAACAA TAACAGCAGT AGTAGAAGTG GGAGCAACAG CTGGAGCAAC 
ACTTGCATGG TCAAGCTGCA GAGCTTGGAG CCCGTTAGCC TGTCCGTTCA TTCGCGCACC 
ATTGGTAAGG TTAAGTCGCC ATTCATTGAA AAGCAGCAGG AGAAACCACA CACCGAGAAG 
CTAGCGAACG GAACCAGCGA CAGTGGCTCC GATTCGGGCA AGGAGAACTG CGCCTCCAAC 
GAGCAGGAAC ATCTGCAGCA CCAGCAGTCG CAGGACGAGG ATCGAGCTCC GCCCAGGGTG 
TCCGAGATGC GGAGGAAAAT CAGCCGACTG GTGCAACAAC AGCAGCCGCA ACCACAGCCC 
GTCAATCGGC GGCACACCAC CGAGATCACC TCCGTGACCG TTGGAACGCG TTCGCCCAGT 
GTGGACGATC GCTTTGCCAA GTACTTTGGT GTTAAGGAGA GCTGCAACAG CACGACCACC 
GTGCATAAAA CGCAATCGCT GCCACCCAGC CAAGTTGAGG CCACCAATGC GCACGTGAAA 
CTACCGCAGA TCACTACCGT GGTTTCCAAA CGGCGCGAGC TGCTCAAGAG GACGCGACCC 
CTGTCCATGG ATCACTACTC CAGCGGCTGC ACCAGTCCCA CAGCCATGCC AAGTCCCAAG 
AGAGCACATT CGCCCGTCGG CCGGCGGAAG GCCGCCATTT CCAGCATCGA GGACATTGAG 
GTCACGCCGG ATGAACTCCG GGCCGCTGGC AGGAACTTCA AGCTGTTGTA CGGCGAGTTA 
TTGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Svil ( NM_001169858.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163329.1 Drosophila melanogaster supervillin, transcript variant E (Svil), mRNA.

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CloneID	OFa19997
Clone ID Related Accession (Same CDS sequence)	NM_001259638.1 , NM_001169858.2
Accession Version	NM_001169858.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	supervillin, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001169858.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCAGCG GCAACTCGGG CGGCAGCAGT GGCAATCCCA AGCAGCAGCA GCAGCAGCAG 
CAGCAACAAC AGCTAGAGCA GCTGGGACAC CAGACACATC TTGTGCTAGG AGCATCAAAG 
GAGTTGCCCG CGGTCAGCAG CAGCAGCGAT GAAGAGGGAT CCCTGCGGAC TCTCCACTCC 
AGTCGCAAGG TGACCAATCT GAATGCCAAG TCCACCTCTC TGCCACCCGA GGAAGGACTC 
TCGGGTGATG TCTCCCTGGA GTCGTCCTCC AACCGACTGA ATCTGCGATT GGCCAGGAGC 
CTGGAGCCCT CAGTCCGCAA TATCACTGCA TCCGCCTCCA AGGAAGTACA GAGCTATGAT 
GCTGGGGAGC CTGTGAATAC CAATCGATTG TTTGTGGAAC CGCCCAAGAT ACCACTGTCT 
GCCATCCGAA ACTCTCTTGA CGATCACGAC CTGGATATTG AGGAGTGCGA CAGCACCACC 
CAGTTCGATC AGGGGGATAT CTACGATACC CTGGAACGCT GGAGCCATGG TGTCAGCTTG 
GGGGCACATC TATTGAACTG CGCGAATGGC CACGATCAAT CTGATCCCGA GGACGACCAT 
CAAACCGATC ATCATCAGCA TGCACATCAA TCATTGCCAG TAGAACCCAA GTCTCATCAT 
CATCAGCACC ACCAGGCCAC CAATGCGCGC AGATTCATAA CGCGCACCAC GCGTTCCGCC 
AGTCGCCAGA GTTCCGCCGG ACACTCCCCA CCTCCGCCGC TGCATCGCAG TGACGACTCC 
TCCAGCAGTT CACCCTCACC GACACGCAGA CGGAATAAGC CAGGTGGTGC TCCTGCCGCC 
ACAGATCCAC CATCATCGTC GGCGAACGGG AATAGTTCCG CTTATGAGAC GGCAGCCACC 
ACTGTGATGA CCCATGACCA GCAGCGAGAT CCTCCAGTTG AAGTTCCTAA GGCTCTAAAC 
GTCGAGCCAC CGCAGCCAGA AGACATCGTC CTGGGCTCCT GCCCCACTGC ATCTGCCCAC 
TCAGATAAAA AGAAGGAAGA GGAGCAACGC AATCGCTACA GTTCGCCCGT TTCACCAAAA 
GCCTCCAGCA ACGGCGGAGT CCAAGCACAA CGTCGTCTTA GCAACTCGGA GCTATCGCCC 
AAGTCGCCAG GTGCCAGTGG ATTCACTTCG GAGCCAGTCG CGTCAGCCAC AGCGAACAGA 
GTCCCGACGA GATCTGCCTC CCATCCTGTG GACACACCGC CCACACAAAG GCGACAGCCT 
CTGGTGGCCG TGAGTTCGGG CATCTCCATG AAGCAGAGTC TCAGCGAGCT GCACTTCACG 
GGTGGAGCAG TCAGTCCGCC CAAGGCTACG GCGGAATGCA CTCTAAGGAT GCGACCACGT 
AGCTCCACGA TGAGCACCGA GAACACGGAG AATGGCCGAC GTGACCACTT GGCTAATCTG 
CAACTAAGTC CTGTGCAAAA GCAAAAGCGA ACGCCCGAAG TATCACCACG CCGTTTCGGA 
GATCGCGACA AGTCGGCCAA ACGGAAATCG AATTTGAATC GTTCGTTGAC CGAGGAAGCC 
ACCAAGGATG GCGATGAGGT TGCCTCTTCG ACCCGCCGGC GCCGACGTCG TGAACTGGGA 
ATGGCTCGTC CCCTGGTTCC GGGCGATGAT GACGCCCTAA GAGATTTGCT AACGCCGCAG 
AAGAGTTCCT GCAGCGAGTC AACCAACTCC ATATCCGAAC CCCATCTCCA GTTCATACCA 
GCCGTTCGTC ATCCGGTGGT TCCAGTACGC ACCATGACTG CTGTGCTGCC TGCACCACGA 
CCGCTGCATA CTCCTGCGGC CCGCAACGTC AGCCAGAAGT TCAATGAAAG GACCAAAACA 
GATCTGGCCG AGATCAAGAA GATTGCTGAG CAATCGGAGG CGCAGGTGCA ACAGCCTTCG 
GTGGATGATA ACCAGAAAAT GTCAGCCATA ATGCGCAGGA AGAGCACGGA TGAGGGTGGA 
TCAGCTTTGG GGGCAACAAA CCAGAATTCA CCACCTGCCC CAAACCAAAT TGTATCCATA 
CTGAAGAAGA AGGAACATGG ACTTGGCGAG TGCAACTCCA GTGCTTCAAG CAATCCCTCG 
CCGGTGACCT TCTCTTCTAG CGTGGTGGAT CATCCTTTGG CTGGAGCTGG CCGGTGCAAA 
CGGCAGGGAA TACTCAAGAA GAGATCGAGT CTAGATGAAT CGCGCTACTA CTCCCGATCG 
CATTCACCCG ACGAACGTAG TATTCTCATT AAGTCGGCCA GGAGGAATTC CCTGGAGGAA 
GCTGGCACTG GATTGAGTCC GGCTCAGGCC CATGGAATCC TCAAGCAGAG CAGCTACGAC 
AGCAGCAAGA GCGACGGCTG CCCTTCGGCC ACGGAGAGCC AGCCACACAG TATCCTGAAA 
AAGAAGGATT CGCTATCGAC ACCTTCGGAC GGAGGATGCC ACAAGCATGT CTCTATATCG 
CAAGCTGTAA CTTTAGCTGC TGCTGAACTG GCTGCCCATG ATGGAATCAC CTTCGATGAT 
GGTGAGGAGC ATGAGATACG ACCGATCCTA AAGCAGGAGA GCACCTCCAG CGAGGAGGCT 
GTGCGTCCGC CAAAGCCCAT ACTCAAAAAG AAATCCTTTG GCGAGGCGGA TGAACACGAA 
ATACGTCCTA TATTGAAGAG CTCTCGTAAA AGCAGTCGAG AGGAGTTCGA TTTGAGTGGC 
TTGGAAAACG AGGTCAACGA TTCCCTATCG TCAATCCTAA AGACAGACTC GCCGTCCAAG 
CGTCGGTCGC TCGGTTCAGC TTTGCATGAT TTGGAGGAGT CCACCACTTC CCCGAGCATG 
CTCAAGAGGC GCACCCGTTC GCTGGAACGT CAAGATGTCC AGCCTGGCAT GGATTTGGCA 
GCTGCACTGG ATGCCATCGC CACATCACAG GCTACTCCCA GCACCCCGGT GGACTTCGTG 
ACCACACCAA CAAGTATATC GGTGGCGGAG CGGATCAAGC GCATGGAGAT GCTGTCGACG 
CCCACTTCCC GTGCGGCGGG TGGGGCCAAC AGCAGCTGGG AATCGCCACT GCAGGCATCG 
TCAGCAGAGC ATGGAGCACC CACCACCCCA AGGGTTCTCA AGCCAAGTGT GCTGCGCAGA 
GATTTGCAGA GGGAGCGGTA CAAAACGCAA CCCGTGACCA ACGAGGAGAT GAGCTTCTTC 
AAAGCCAACT CCGCGCCCTT CGACATCTCG GCTACATCGG CCTTCAAGCC CACCAAGCCG 
CTGGACATCC GCTTGACCCA CGCACCGCAT GCACCGCTGA TTTCGCCGAT CACCGGACAG 
CCACTCAGCC ACGTGCCCGC GCCACTGCTG CTATCCTCGT CGACGAACTC GGGAGCGGGA 
TCCTCGTTCC GTCTGGCCCG CAGCTCCACC CAACCGCTGC AGTCGCCGCG AGTTGTCCAC 
CTGGCCGGTC AGGCTACTGG TGGCTCCGCT TTGGCACGCC AGAACTCGGT GAACGACAGC 
ATCAATCTCA GCGAGCAGCT AACCCTATCC ATCGGCACGG ACAGCGAGCA CATCTTTGAG 
ATGTCCGCTT TGGAGGAGGA CTCGGCTATT CAGTCGCTGA GCGACGAGAC AGCCGCTTCC 
GCATCTGCCA ACTCAACATC CACGACCACT GCAACCACCA TGACACCGCC CAGTGGCCTG 
ACGCGGAGCA ACAGCGTTCG GGCCAGGGCC AATATGTTCC AGCAATTGCA GGAGCAGTCG 
CGCAACCAAC GGGCAACGGG AACCACCAAC TCCCGTCAAT CTCCACCAGC GTCGTCGGCG 
GAGGTGTCTT CAAGTGCAGC CGACAATTCC ATGCACAGTG ACGAGCGGTC CACGCCAAAT 
GCAACGATAG ATGCGGAATT CGAACCCTCA TCCCTGTCGC TGGCTGAGCG ACTGGCCTTC 
TTCAGCAATC TGTGCGAGAG CGGCGGAGGA GGTGGGAGTA CCAGTGCCGG TGGAAGGTAC 
CGCAGTCGCA CCAGCAGCTA TTCAAGGAGT CCGCCCGGCG ATCGCACCAC TCCCAGGAGC 
AGCACCACCG CCAGCAGTGT CAGTATGACG CCCACGCCCA TGGATTACTC TCCGATTCCC 
CACGACAAGG AGCCATCTAG TCTCACGCTG GAGAGCATCA TCGAGCCCGA GCAGGAGGAG 
GTCAAGGAGC AGGTGGACCA GGATGAGGTC AAGTTGAGGA AGAAGGAGCC AGTCCAGAGG 
GAATCTTCCC AGCACAATGG CTTCCACGTG CTGACCAGGT CCGCCACCGA ACCGCCGTCG 
TCCACCTCGC CCCTGCGCAT CAGCATACGG ACTGTGGGAA AGCTAGTCCT GCCCGATACC 
TTCCGCGGCG ATCGCAACAA TAACAGCAGT AGTAGAAGTG GGAGCAACAG CTGGAGCAAC 
ACTTGCATGG TCAAGCTGCA GAGCTTGGAG CCCGTTAGCC TGTCCGTTCA TTCGCGCACC 
ATTGGTAAGG TTAAGTCGCC ATTCATTGAA AAGCAGCAGG AGAAACCACA CACCGAGAAG 
CTAGCGAACG GAACCAGCGA CAGTGGCTCC GATTCGGGCA AGGAGAACTG CGCCTCCAAC 
GAGCAGGAAC ATCTGCAGCA CCAGCAGTCG CAGGACGAGG ATCGAGCTCC GCCCAGGGTG 
TCCGAGATGC GGAGGAAAAT CAGCCGACTG GTGCAACAAC AGCAGCCGCA ACCACAGCCC 
GTCAATCGGC GGCACACCAC CGAGATCACC TCCGTGACCG TTGGAACGCG TTCGCCCAGT 
GTGGACGATC GCTTTGCCAA GTACTTTGGT GTTAAGGAGA GCTGCAACAG CACGACCACC 
GTGCATAAAA CGCAATCGCT GCCACCCAGC CAAGTTGAGG CCACCAATGC GCACGTGAAA 
CTACCGCAGA TCACTACCGT GGTTTCCAAA CGGCGCGAGC TGCTCAAGAG GACGCGACCC 
CTGTCCATGG ATCACTACTC CAGCGGCTGC ACCAGTCCCA CAGCCATGCC AAGTCCCAAG 
AGAGCACATT CGCCCGTCGG CCGGCGGAAG GCCGCCATTT CCAGCATCGA GGACATTGAG 
GTCACGCCGG ATGAACTCCG GGCCGCTGGC AGGAACTTCA AGCTGTTGTA CGGCGAGTTA 
TTGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163329.1
CDS	1216..6324
Translation	MSSGNSGGSSGNPKQQQQQQQQQQLEQLGHQTHLVLGASKELPAVSSSSDEEGSLRTLHSSRKVTNLNAKSTSLPPEEGLSGDVSLESSSNRLNLRLARSLEPSVRNITASASKEVQSYDAGEPVNTNRLFVEPPKIPLSAIRNSLDDHDLDIEECDSTTQFDQGDIYDTLERWSHGVSLGAHLLNCANGHDQSDPEDDHQTDHHQHAHQSLPVEPKSHHHQHHQATNARRFITRTTRSASRQSSAGHSPPPPLHRSDDSSSSSPSPTRRRNKPGGAPAATDPPSSSANGNSSAYETAATTVMTHDQQRDPPVEVPKALNVEPPQPEDIVLGSCPTASAHSDKKKEEEQRNRYSSPVSPKASSNGGVQAQRRLSNSELSPKSPGASGFTSEPVASATANRVPTRSASHPVDTPPTQRRQPLVAVSSGISMKQSLSELHFTGGAVSPPKATAECTLRMRPRSSTMSTENTENGRRDHLANLQLSPVQKQKRTPEVSPRRFGDRDKSAKRKSNLNRSLTEEATKDGDEVASSTRRRRRRELGMARPLVPGDDDALRDLLTPQKSSCSESTNSISEPHLQFIPAVRHPVVPVRTMTAVLPAPRPLHTPAARNVSQKFNERTKTDLAEIKKIAEQSEAQVQQPSVDDNQKMSAIMRRKSTDEGGSALGATNQNSPPAPNQIVSILKKKEHGLGECNSSASSNPSPVTFSSSVVDHPLAGAGRCKRQGILKKRSSLDESRYYSRSHSPDERSILIKSARRNSLEEAGTGLSPAQAHGILKQSSYDSSKSDGCPSATESQPHSILKKKDSLSTPSDGGCHKHVSISQAVTLAAAELAAHDGITFDDGEEHEIRPILKQESTSSEEAVRPPKPILKKKSFGEADEHEIRPILKSSRKSSREEFDLSGLENEVNDSLSSILKTDSPSKRRSLGSALHDLEESTTSPSMLKRRTRSLERQDVQPGMDLAAALDAIATSQATPSTPVDFVTTPTSISVAERIKRMEMLSTPTSRAAGGANSSWESPLQASSAEHGAPTTPRVLKPSVLRRDLQRERYKTQPVTNEEMSFFKANSAPFDISATSAFKPTKPLDIRLTHAPHAPLISPITGQPLSHVPAPLLLSSSTNSGAGSSFRLARSSTQPLQSPRVVHLAGQATGGSALARQNSVNDSINLSEQLTLSIGTDSEHIFEMSALEEDSAIQSLSDETAASASANSTSTTTATTMTPPSGLTRSNSVRARANMFQQLQEQSRNQRATGTTNSRQSPPASSAEVSSSAADNSMHSDERSTPNATIDAEFEPSSLSLAERLAFFSNLCESGGGGGSTSAGGRYRSRTSSYSRSPPGDRTTPRSSTTASSVSMTPTPMDYSPIPHDKEPSSLTLESIIEPEQEEVKEQVDQDEVKLRKKEPVQRESSQHNGFHVLTRSATEPPSSTSPLRISIRTVGKLVLPDTFRGDRNNNSSSRSGSNSWSNTCMVKLQSLEPVSLSVHSRTIGKVKSPFIEKQQEKPHTEKLANGTSDSGSDSGKENCASNEQEHLQHQQSQDEDRAPPRVSEMRRKISRLVQQQQPQPQPVNRRHTTEITSVTVGTRSPSVDDRFAKYFGVKESCNSTTTVHKTQSLPPSQVEATNAHVKLPQITTVVSKRRELLKRTRPLSMDHYSSGCTSPTAMPSPKRAHSPVGRRKAAISSIEDIEVTPDELRAAGRNFKLLYGELLG

Target ORF information:

RefSeq Version	NM_001169858.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster supervillin, transcript variant E (Svil), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169858.2

ORF Insert Sequence:

ATGAGCAGCG GCAACTCGGG CGGCAGCAGT GGCAATCCCA AGCAGCAGCA GCAGCAGCAG 
CAGCAACAAC AGCTAGAGCA GCTGGGACAC CAGACACATC TTGTGCTAGG AGCATCAAAG 
GAGTTGCCCG CGGTCAGCAG CAGCAGCGAT GAAGAGGGAT CCCTGCGGAC TCTCCACTCC 
AGTCGCAAGG TGACCAATCT GAATGCCAAG TCCACCTCTC TGCCACCCGA GGAAGGACTC 
TCGGGTGATG TCTCCCTGGA GTCGTCCTCC AACCGACTGA ATCTGCGATT GGCCAGGAGC 
CTGGAGCCCT CAGTCCGCAA TATCACTGCA TCCGCCTCCA AGGAAGTACA GAGCTATGAT 
GCTGGGGAGC CTGTGAATAC CAATCGATTG TTTGTGGAAC CGCCCAAGAT ACCACTGTCT 
GCCATCCGAA ACTCTCTTGA CGATCACGAC CTGGATATTG AGGAGTGCGA CAGCACCACC 
CAGTTCGATC AGGGGGATAT CTACGATACC CTGGAACGCT GGAGCCATGG TGTCAGCTTG 
GGGGCACATC TATTGAACTG CGCGAATGGC CACGATCAAT CTGATCCCGA GGACGACCAT 
CAAACCGATC ATCATCAGCA TGCACATCAA TCATTGCCAG TAGAACCCAA GTCTCATCAT 
CATCAGCACC ACCAGGCCAC CAATGCGCGC AGATTCATAA CGCGCACCAC GCGTTCCGCC 
AGTCGCCAGA GTTCCGCCGG ACACTCCCCA CCTCCGCCGC TGCATCGCAG TGACGACTCC 
TCCAGCAGTT CACCCTCACC GACACGCAGA CGGAATAAGC CAGGTGGTGC TCCTGCCGCC 
ACAGATCCAC CATCATCGTC GGCGAACGGG AATAGTTCCG CTTATGAGAC GGCAGCCACC 
ACTGTGATGA CCCATGACCA GCAGCGAGAT CCTCCAGTTG AAGTTCCTAA GGCTCTAAAC 
GTCGAGCCAC CGCAGCCAGA AGACATCGTC CTGGGCTCCT GCCCCACTGC ATCTGCCCAC 
TCAGATAAAA AGAAGGAAGA GGAGCAACGC AATCGCTACA GTTCGCCCGT TTCACCAAAA 
GCCTCCAGCA ACGGCGGAGT CCAAGCACAA CGTCGTCTTA GCAACTCGGA GCTATCGCCC 
AAGTCGCCAG GTGCCAGTGG ATTCACTTCG GAGCCAGTCG CGTCAGCCAC AGCGAACAGA 
GTCCCGACGA GATCTGCCTC CCATCCTGTG GACACACCGC CCACACAAAG GCGACAGCCT 
CTGGTGGCCG TGAGTTCGGG CATCTCCATG AAGCAGAGTC TCAGCGAGCT GCACTTCACG 
GGTGGAGCAG TCAGTCCGCC CAAGGCTACG GCGGAATGCA CTCTAAGGAT GCGACCACGT 
AGCTCCACGA TGAGCACCGA GAACACGGAG AATGGCCGAC GTGACCACTT GGCTAATCTG 
CAACTAAGTC CTGTGCAAAA GCAAAAGCGA ACGCCCGAAG TATCACCACG CCGTTTCGGA 
GATCGCGACA AGTCGGCCAA ACGGAAATCG AATTTGAATC GTTCGTTGAC CGAGGAAGCC 
ACCAAGGATG GCGATGAGGT TGCCTCTTCG ACCCGCCGGC GCCGACGTCG TGAACTGGGA 
ATGGCTCGTC CCCTGGTTCC GGGCGATGAT GACGCCCTAA GAGATTTGCT AACGCCGCAG 
AAGAGTTCCT GCAGCGAGTC AACCAACTCC ATATCCGAAC CCCATCTCCA GTTCATACCA 
GCCGTTCGTC ATCCGGTGGT TCCAGTACGC ACCATGACTG CTGTGCTGCC TGCACCACGA 
CCGCTGCATA CTCCTGCGGC CCGCAACGTC AGCCAGAAGT TCAATGAAAG GACCAAAACA 
GATCTGGCCG AGATCAAGAA GATTGCTGAG CAATCGGAGG CGCAGGTGCA ACAGCCTTCG 
GTGGATGATA ACCAGAAAAT GTCAGCCATA ATGCGCAGGA AGAGCACGGA TGAGGGTGGA 
TCAGCTTTGG GGGCAACAAA CCAGAATTCA CCACCTGCCC CAAACCAAAT TGTATCCATA 
CTGAAGAAGA AGGAACATGG ACTTGGCGAG TGCAACTCCA GTGCTTCAAG CAATCCCTCG 
CCGGTGACCT TCTCTTCTAG CGTGGTGGAT CATCCTTTGG CTGGAGCTGG CCGGTGCAAA 
CGGCAGGGAA TACTCAAGAA GAGATCGAGT CTAGATGAAT CGCGCTACTA CTCCCGATCG 
CATTCACCCG ACGAACGTAG TATTCTCATT AAGTCGGCCA GGAGGAATTC CCTGGAGGAA 
GCTGGCACTG GATTGAGTCC GGCTCAGGCC CATGGAATCC TCAAGCAGAG CAGCTACGAC 
AGCAGCAAGA GCGACGGCTG CCCTTCGGCC ACGGAGAGCC AGCCACACAG TATCCTGAAA 
AAGAAGGATT CGCTATCGAC ACCTTCGGAC GGAGGATGCC ACAAGCATGT CTCTATATCG 
CAAGCTGTAA CTTTAGCTGC TGCTGAACTG GCTGCCCATG ATGGAATCAC CTTCGATGAT 
GGTGAGGAGC ATGAGATACG ACCGATCCTA AAGCAGGAGA GCACCTCCAG CGAGGAGGCT 
GTGCGTCCGC CAAAGCCCAT ACTCAAAAAG AAATCCTTTG GCGAGGCGGA TGAACACGAA 
ATACGTCCTA TATTGAAGAG CTCTCGTAAA AGCAGTCGAG AGGAGTTCGA TTTGAGTGGC 
TTGGAAAACG AGGTCAACGA TTCCCTATCG TCAATCCTAA AGACAGACTC GCCGTCCAAG 
CGTCGGTCGC TCGGTTCAGC TTTGCATGAT TTGGAGGAGT CCACCACTTC CCCGAGCATG 
CTCAAGAGGC GCACCCGTTC GCTGGAACGT CAAGATGTCC AGCCTGGCAT GGATTTGGCA 
GCTGCACTGG ATGCCATCGC CACATCACAG GCTACTCCCA GCACCCCGGT GGACTTCGTG 
ACCACACCAA CAAGTATATC GGTGGCGGAG CGGATCAAGC GCATGGAGAT GCTGTCGACG 
CCCACTTCCC GTGCGGCGGG TGGGGCCAAC AGCAGCTGGG AATCGCCACT GCAGGCATCG 
TCAGCAGAGC ATGGAGCACC CACCACCCCA AGGGTTCTCA AGCCAAGTGT GCTGCGCAGA 
GATTTGCAGA GGGAGCGGTA CAAAACGCAA CCCGTGACCA ACGAGGAGAT GAGCTTCTTC 
AAAGCCAACT CCGCGCCCTT CGACATCTCG GCTACATCGG CCTTCAAGCC CACCAAGCCG 
CTGGACATCC GCTTGACCCA CGCACCGCAT GCACCGCTGA TTTCGCCGAT CACCGGACAG 
CCACTCAGCC ACGTGCCCGC GCCACTGCTG CTATCCTCGT CGACGAACTC GGGAGCGGGA 
TCCTCGTTCC GTCTGGCCCG CAGCTCCACC CAACCGCTGC AGTCGCCGCG AGTTGTCCAC 
CTGGCCGGTC AGGCTACTGG TGGCTCCGCT TTGGCACGCC AGAACTCGGT GAACGACAGC 
ATCAATCTCA GCGAGCAGCT AACCCTATCC ATCGGCACGG ACAGCGAGCA CATCTTTGAG 
ATGTCCGCTT TGGAGGAGGA CTCGGCTATT CAGTCGCTGA GCGACGAGAC AGCCGCTTCC 
GCATCTGCCA ACTCAACATC CACGACCACT GCAACCACCA TGACACCGCC CAGTGGCCTG 
ACGCGGAGCA ACAGCGTTCG GGCCAGGGCC AATATGTTCC AGCAATTGCA GGAGCAGTCG 
CGCAACCAAC GGGCAACGGG AACCACCAAC TCCCGTCAAT CTCCACCAGC GTCGTCGGCG 
GAGGTGTCTT CAAGTGCAGC CGACAATTCC ATGCACAGTG ACGAGCGGTC CACGCCAAAT 
GCAACGATAG ATGCGGAATT CGAACCCTCA TCCCTGTCGC TGGCTGAGCG ACTGGCCTTC 
TTCAGCAATC TGTGCGAGAG CGGCGGAGGA GGTGGGAGTA CCAGTGCCGG TGGAAGGTAC 
CGCAGTCGCA CCAGCAGCTA TTCAAGGAGT CCGCCCGGCG ATCGCACCAC TCCCAGGAGC 
AGCACCACCG CCAGCAGTGT CAGTATGACG CCCACGCCCA TGGATTACTC TCCGATTCCC 
CACGACAAGG AGCCATCTAG TCTCACGCTG GAGAGCATCA TCGAGCCCGA GCAGGAGGAG 
GTCAAGGAGC AGGTGGACCA GGATGAGGTC AAGTTGAGGA AGAAGGAGCC AGTCCAGAGG 
GAATCTTCCC AGCACAATGG CTTCCACGTG CTGACCAGGT CCGCCACCGA ACCGCCGTCG 
TCCACCTCGC CCCTGCGCAT CAGCATACGG ACTGTGGGAA AGCTAGTCCT GCCCGATACC 
TTCCGCGGCG ATCGCAACAA TAACAGCAGT AGTAGAAGTG GGAGCAACAG CTGGAGCAAC 
ACTTGCATGG TCAAGCTGCA GAGCTTGGAG CCCGTTAGCC TGTCCGTTCA TTCGCGCACC 
ATTGGTAAGG TTAAGTCGCC ATTCATTGAA AAGCAGCAGG AGAAACCACA CACCGAGAAG 
CTAGCGAACG GAACCAGCGA CAGTGGCTCC GATTCGGGCA AGGAGAACTG CGCCTCCAAC 
GAGCAGGAAC ATCTGCAGCA CCAGCAGTCG CAGGACGAGG ATCGAGCTCC GCCCAGGGTG 
TCCGAGATGC GGAGGAAAAT CAGCCGACTG GTGCAACAAC AGCAGCCGCA ACCACAGCCC 
GTCAATCGGC GGCACACCAC CGAGATCACC TCCGTGACCG TTGGAACGCG TTCGCCCAGT 
GTGGACGATC GCTTTGCCAA GTACTTTGGT GTTAAGGAGA GCTGCAACAG CACGACCACC 
GTGCATAAAA CGCAATCGCT GCCACCCAGC CAAGTTGAGG CCACCAATGC GCACGTGAAA 
CTACCGCAGA TCACTACCGT GGTTTCCAAA CGGCGCGAGC TGCTCAAGAG GACGCGACCC 
CTGTCCATGG ATCACTACTC CAGCGGCTGC ACCAGTCCCA CAGCCATGCC AAGTCCCAAG 
AGAGCACATT CGCCCGTCGG CCGGCGGAAG GCCGCCATTT CCAGCATCGA GGACATTGAG 
GTCACGCCGG ATGAACTCCG GGCCGCTGGC AGGAACTTCA AGCTGTTGTA CGGCGAGTTA 
TTGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Svil ( NM_001259639.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246568.1 Drosophila melanogaster supervillin, transcript variant S (Svil), mRNA.

Price & Availability↑

CloneID	OFa19998
Clone ID Related Accession (Same CDS sequence)	NM_001259639.1
Accession Version	NM_001259639.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	supervillin, isoform S
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCAGCG GCAACTCGGG CGGCAGCAGT GGCAATCCCA AGCAGCAGCA GCAGCAGCAG 
CAGCAACAAC AGCTAGAGCA GCTGGGACAC CAGACACATC TTGTGCTAGG AGCATCAAAG 
GAGTTGCCCG CGGTCAGCAG CAGCAGCGAT GAAGAGGGAT CCCTGCGGAC TCTCCACTCC 
AGTCGCAAGG TGACCAATCT GAATGCCAAG TCCACCTCTC TGCCACCCGA GGAAGGACTC 
TCGGGTGATG TCTCCCTGGA GTCGTCCTCC AACCGACTGA ATCTGCGATT GGCCAGGAGC 
CTGGAGCCCT CAGTCCGCAA TATCACTGCA TCCGCCTCCA AGGAAGTACA GAGCTATGAT 
GCTGGGGAGC CTGTGAATAC CAATCGATTG TTTGTGGAAC CGCCCAAGAT ACCACTGTCT 
GCCATCCGAA ACTCTCTTGA CGATCACGAC CTGGATATTG AGGAGTGCGA CAGCACCACC 
CAGTTCGATC AGGGGGATAT CTACGATACC CTGGAACGCT GGAGCCATGG TGTCAGCTTG 
GGGGCACATC TATTGAACTG CGCGAATGGC CACGATCAAT CTGATCCCGA GGACGACCAT 
CAAACCGATC ATCATCAGCA TGCACATCAA TCATTGCCAG TAGAACCCAA GTCTCATCAT 
CATCAGCACC ACCAGGCCAC CAATGCGCGC AGATTCATAA CGCGCACCAC GCGTTCCGCC 
AGTCGCCAGA GTTCCGCCGG ACACTCCCCA CCTCCGCCGC TGCATCGCAG TGACGACTCC 
TCCAGCAGTT CACCCTCACC GACACGCAGA CGGAATAAGC CAGGTGGTGC TCCTGCCGCC 
ACAGATCCAC CATCATCGTC GGCGAACGGG AATAGTTCCG CTTATGAGAC GGCAGCCACC 
ACTGTGATGA CCCATGACCA GCAGCGAGAT CCTCCAGTTG AAGTTCCTAA GGCTCTAAAC 
GTCGAGCCAC CGCAGCCAGA AGACATCGTC CTGGGCTCCT GCCCCACTGC ATCTGCCCAC 
TCAGCAGAGA GCCAAAGTCG AGGAAAAGCT GGCAAATGCA GTATTAATTG CACCTACAGC 
CACAGCCACA GCACCAACAA CAACTGCAAC GCGCGACAAC GCAACAGCAG CAACATCAGC 
AGCAACAACA CCATCAGCAG CAACAGCAGC AGCAGCAAAA GCACTTGCAA CAACAATAGC 
AAACAGCAAC ACGCCACGCC CATGCCGCCC CTAGCTAATG CCAACAAAGC CCCGTTAATT 
GTGCTTAAGA CAGCCGCATC TGCAGCAGCA TTGCCGTCCA TTGCCGCAGC AGCAACATCC 
AGCGGCACGG CAGCAACATC TGGTGGTGTT GGTTCGCCCA TCAAGCGTCG CAAGAATCCG 
CTGTCGCCGC TGCTCCTCGG CAGCCAGTGC CAGTCCACCA TTGCGCCTCC GCAGGCCATT 
GCCAGTCCGC TCCTGAGCGG CGGCAGCTGC TCCTCCTCGA CCACCTCGCC CCAATCCTCG 
CCAGCGGTTC GCACCACCAA GGCGAGTCGT CTGCGCGCCG CCGCGCTTGA TAAAAAGAAG 
GAAGAGGAGC AACGCAATCG CTACAGTTCG CCCGTTTCAC CAAAAGCCTC CAGCAACGGC 
GGAGTCCAAG CACAACGTCG TCTTAGCAAC TCGGAGCTAT CGCCCAAGTC GCCAGGTGCC 
AGTGGATTCA CTTCGGAGCC AGTCGCGTCA GCCACAGCGA ACAGAGTCCC GACGAGATCT 
GCCTCCCATC CTGTGGACAC ACCGCCCACA CAAAGGCGAC AGCCTCTGGT GGCCGTGAGT 
TCGGGCATCT CCATGAAGCA GAGTCTCAGC GAGCTGCACT TCACGGGTGG AGCAGTCAGT 
CCGCCCAAGG CTACGGCGGA ATGCACTCTA AGGATGCGAC CACGTAGCTC CACGATGAGC 
ACCGAGAACA CGGAGAATGG CCGACGTGAC CACTTGGCTA ATCTGCAACT AAGTCCTGTG 
CAAAAGCAAA AGCGAACGCC CGAAGTATCA CCACGCCGTT TCGGAGATCG CGACAAGTCG 
GCCAAACGGA AATCGAATTT GAATCGTTCG TTGACCGAGG AAGCCACCAA GGATGGCGAT 
GAGGTTGCCT CTTCGACCCG CCGGCGCCGA CGTCGTGAAC TGGGAATGGC TCGTCCCCTG 
GTTCCGGGCG ATGATGACGC CCTAAGAGAT TTGCTAACGC CGCAGAAGAG TTCCTGCAGC 
GAGTCAACCA ACTCCATATC CGAACCCCAT CTCCAGTTCA TACCAGCCGT TCGTCATCCG 
GTGGTTCCAG TACGCACCAT GACTGCTGTG CTGCCTGCAC CACGACCGCT GCATACTCCT 
GCGGCCCGCA ACGTCAGCCA GAAGTTCAAT GAAAGGACCA AAACAGATCT GGCCGAGATC 
AAGAAGATTG CTGAGCAATC GGAGGCGCAG GTGCAACAGC CTTCGGTGGA TGATAACCAG 
AAAATGTCAG CCATAATGCG CAGGAAGAGC ACGGATGAGG GTGGATCAGC TTTGGGGGCA 
ACAAACCAGA ATTCACCACC TGCCCCAAAC CAAATTGTAT CCATACTGAA GAAGAAGGAA 
CATGGACTTG GCGAGTGCAA CTCCAGTGCT TCAAGCAATC CCTCGCCGGT GACCTTCTCT 
TCTAGCGTGG TGGATCATCC TTTGGCTGGA GCTGGCCGGT GCAAACGGCA GGGAATACTC 
AAGAAGAGAT CGAGTCTAGA TGAATCGCGC TACTACTCCC GATCGCATTC ACCCGACGAA 
CGTAGTATTC TCATTAAGTC GGCCAGGAGG AATTCCCTGG AGGAAGCTGG CACTGGATTG 
AGTCCGGCTC AGGCCCATGG AATCCTCAAG CAGAGCAGCT ACGACAGCAG CAAGAGCGAC 
GGCTGCCCTT CGGCCACGGA GAGCCAGCCA CACAGTATCC TGAAAAAGAA GGATTCGCTA 
TCGACACCTT CGGACGGAGG ATGCCACAAG CATGTCTCTA TATCGCAAGC TGTAACTTTA 
GCTGCTGCTG AACTGGCTGC CCATGATGGA ATCACCTTCG ATGATGGTGA GGAGCATGAG 
ATACGACCGA TCCTAAAGCA GGAGAGCACC TCCAGCGAGG AGGCTGTGCG TCCGCCAAAG 
CCCATACTCA AAAAGAAATC CTTTGGCGAG GCGGATGAAC ACGAAATACG TCCTATATTG 
AAGAGCTCTC GTAAAAGCAG TCGAGAGGAG TTCGATTTGA GTGGCTTGGA AAACGAGGTC 
AACGATTCCC TATCGTCAAT CCTAAAGACA GACTCGCCGT CCAAGCGTCG GTCGCTCGGT 
TCAGCTTTGC ATGATTTGGA GGAGTCCACC ACTTCCCCGA GCATGCTCAA GAGGCGCACC 
CGTTCGCTGG AACGTCAAGA TGTCCAGCCT GGCATGGATT TGGCAGCTGC ACTGGATGCC 
ATCGCCACAT CACAGGCTAC TCCCAGCACC CCGGTGGACT TCGTGACCAC ACCAACAAGT 
ATATCGGTGG CGGAGCGGAT CAAGCGCATG GAGATGCTGT CGACGCCCAC TTCCCGTGCG 
GCGGGTGGGG CCAACAGCAG CTGGGAATCG CCACTGCAGG CATCGTCAGC AGAGCATGGA 
GCACCCACCA CCCCAAGGGT TCTCAAGCCA AGTGTGCTGC GCAGAGATTT GCAGAGGGAG 
CGGTACAAAA CGCAACCCGT GACCAACGAG GAGATGAGCT TCTTCAAAGC CAACTCCGCG 
CCCTTCGACA TCTCGGCTAC ATCGGCCTTC AAGCCCACCA AGCCGCTGGA CATCCGCTTG 
ACCCACGCAC CGCATGCACC GCTGATTTCG CCGATCACCG GACAGCCACT CAGCCACGTG 
CCCGCGCCAC TGCTGCTATC CTCGTCGACG AACTCGGGAG CGGGATCCTC GTTCCGTCTG 
GCCCGCAGCT CCACCCAACC GCTGCAGTCG CCGCGAGTTG TCCACCTGGC CGGTCAGGCT 
ACTGGTGGCT CCGCTTTGGC ACGCCAGAAC TCGGTGAACG ACAGCATCAA TCTCAGCGAG 
CAGCTAACCC TATCCATCGG CACGGACAGC GAGCACATCT TTGAGATGTC CGCTTTGGAG 
GAGGACTCGG CTATTCAGTC GCTGAGCGAC GAGACAGCCG CTTCCGCATC TGCCAACTCA 
ACATCCACGA CCACTGCAAC CACCATGACA CCGCCCAGTG GCCTGACGCG GAGCAACAGC 
GTTCGGGCCA GGGCCAATAT GTTCCAGCAA TTGCAGGAGC AGTCGCGCAA CCAACGGGCA 
ACGGGAACCA CCAACTCCCG TCAATCTCCA CCAGCGTCGT CGGCGGAGGT GTCTTCAAGT 
GCAGCCGACA ATTCCATGCA CAGTGACGAG CGGTCCACGC CAAATGCAAC GATAGATGCG 
GAATTCGGAA AACCCAGCAC CGAACCACTT AAGAGCATTC TTAAGACGGG AATGCCAGTG 
ATGGGTATGG GACGCGGTTT AATGCCTGTG GATCTCAATG CCGAGCTGAA GAACCGGCTC 
AAACGCTCCA CACATGCCAC TGTTTCCAAT CTCCGCAAAT CTGCCACCTC GGCAGATGCC 
ACTAGAGCAG CTAGTGATGA GGTGGACAAT CCGCAGAGGA ATTTAGCCCA GATCCTGAGG 
AATGTTTCCA AGGAGAACTC CACGCCGAAG CACGATGATG CCGTGAGCCT AGTGCGGAAT 
CTCTCTACTT TGGGACAACC TCGATCGCTG GCCCGTGACG ATGCAGCCGG GAATTGTGCC 
TCGGCATCCG AAGGCGAGAG TTCGGGGGGC CGCGAGATCG AGGCCATAAT CAAGAACAGC 
GCTGTGGCCA GACGTCGCCG GCAGCAACAG CAGCAGTCGC AGCAGCAGAA TCAGCCAGAT 
GGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246568.1
CDS	1293..6158
Translation	MSSGNSGGSSGNPKQQQQQQQQQQLEQLGHQTHLVLGASKELPAVSSSSDEEGSLRTLHSSRKVTNLNAKSTSLPPEEGLSGDVSLESSSNRLNLRLARSLEPSVRNITASASKEVQSYDAGEPVNTNRLFVEPPKIPLSAIRNSLDDHDLDIEECDSTTQFDQGDIYDTLERWSHGVSLGAHLLNCANGHDQSDPEDDHQTDHHQHAHQSLPVEPKSHHHQHHQATNARRFITRTTRSASRQSSAGHSPPPPLHRSDDSSSSSPSPTRRRNKPGGAPAATDPPSSSANGNSSAYETAATTVMTHDQQRDPPVEVPKALNVEPPQPEDIVLGSCPTASAHSAESQSRGKAGKCSINCTYSHSHSTNNNCNARQRNSSNISSNNTISSNSSSSKSTCNNNSKQQHATPMPPLANANKAPLIVLKTAASAAALPSIAAAATSSGTAATSGGVGSPIKRRKNPLSPLLLGSQCQSTIAPPQAIASPLLSGGSCSSSTTSPQSSPAVRTTKASRLRAAALDKKKEEEQRNRYSSPVSPKASSNGGVQAQRRLSNSELSPKSPGASGFTSEPVASATANRVPTRSASHPVDTPPTQRRQPLVAVSSGISMKQSLSELHFTGGAVSPPKATAECTLRMRPRSSTMSTENTENGRRDHLANLQLSPVQKQKRTPEVSPRRFGDRDKSAKRKSNLNRSLTEEATKDGDEVASSTRRRRRRELGMARPLVPGDDDALRDLLTPQKSSCSESTNSISEPHLQFIPAVRHPVVPVRTMTAVLPAPRPLHTPAARNVSQKFNERTKTDLAEIKKIAEQSEAQVQQPSVDDNQKMSAIMRRKSTDEGGSALGATNQNSPPAPNQIVSILKKKEHGLGECNSSASSNPSPVTFSSSVVDHPLAGAGRCKRQGILKKRSSLDESRYYSRSHSPDERSILIKSARRNSLEEAGTGLSPAQAHGILKQSSYDSSKSDGCPSATESQPHSILKKKDSLSTPSDGGCHKHVSISQAVTLAAAELAAHDGITFDDGEEHEIRPILKQESTSSEEAVRPPKPILKKKSFGEADEHEIRPILKSSRKSSREEFDLSGLENEVNDSLSSILKTDSPSKRRSLGSALHDLEESTTSPSMLKRRTRSLERQDVQPGMDLAAALDAIATSQATPSTPVDFVTTPTSISVAERIKRMEMLSTPTSRAAGGANSSWESPLQASSAEHGAPTTPRVLKPSVLRRDLQRERYKTQPVTNEEMSFFKANSAPFDISATSAFKPTKPLDIRLTHAPHAPLISPITGQPLSHVPAPLLLSSSTNSGAGSSFRLARSSTQPLQSPRVVHLAGQATGGSALARQNSVNDSINLSEQLTLSIGTDSEHIFEMSALEEDSAIQSLSDETAASASANSTSTTTATTMTPPSGLTRSNSVRARANMFQQLQEQSRNQRATGTTNSRQSPPASSAEVSSSAADNSMHSDERSTPNATIDAEFGKPSTEPLKSILKTGMPVMGMGRGLMPVDLNAELKNRLKRSTHATVSNLRKSATSADATRAASDEVDNPQRNLAQILRNVSKENSTPKHDDAVSLVRNLSTLGQPRSLARDDAAGNCASASEGESSGGREIEAIIKNSAVARRRRQQQQQSQQQNQPDG

Target ORF information:

RefSeq Version	NM_001259639.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster supervillin, transcript variant S (Svil), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259639.1

ORF Insert Sequence:

ATGAGCAGCG GCAACTCGGG CGGCAGCAGT GGCAATCCCA AGCAGCAGCA GCAGCAGCAG 
CAGCAACAAC AGCTAGAGCA GCTGGGACAC CAGACACATC TTGTGCTAGG AGCATCAAAG 
GAGTTGCCCG CGGTCAGCAG CAGCAGCGAT GAAGAGGGAT CCCTGCGGAC TCTCCACTCC 
AGTCGCAAGG TGACCAATCT GAATGCCAAG TCCACCTCTC TGCCACCCGA GGAAGGACTC 
TCGGGTGATG TCTCCCTGGA GTCGTCCTCC AACCGACTGA ATCTGCGATT GGCCAGGAGC 
CTGGAGCCCT CAGTCCGCAA TATCACTGCA TCCGCCTCCA AGGAAGTACA GAGCTATGAT 
GCTGGGGAGC CTGTGAATAC CAATCGATTG TTTGTGGAAC CGCCCAAGAT ACCACTGTCT 
GCCATCCGAA ACTCTCTTGA CGATCACGAC CTGGATATTG AGGAGTGCGA CAGCACCACC 
CAGTTCGATC AGGGGGATAT CTACGATACC CTGGAACGCT GGAGCCATGG TGTCAGCTTG 
GGGGCACATC TATTGAACTG CGCGAATGGC CACGATCAAT CTGATCCCGA GGACGACCAT 
CAAACCGATC ATCATCAGCA TGCACATCAA TCATTGCCAG TAGAACCCAA GTCTCATCAT 
CATCAGCACC ACCAGGCCAC CAATGCGCGC AGATTCATAA CGCGCACCAC GCGTTCCGCC 
AGTCGCCAGA GTTCCGCCGG ACACTCCCCA CCTCCGCCGC TGCATCGCAG TGACGACTCC 
TCCAGCAGTT CACCCTCACC GACACGCAGA CGGAATAAGC CAGGTGGTGC TCCTGCCGCC 
ACAGATCCAC CATCATCGTC GGCGAACGGG AATAGTTCCG CTTATGAGAC GGCAGCCACC 
ACTGTGATGA CCCATGACCA GCAGCGAGAT CCTCCAGTTG AAGTTCCTAA GGCTCTAAAC 
GTCGAGCCAC CGCAGCCAGA AGACATCGTC CTGGGCTCCT GCCCCACTGC ATCTGCCCAC 
TCAGCAGAGA GCCAAAGTCG AGGAAAAGCT GGCAAATGCA GTATTAATTG CACCTACAGC 
CACAGCCACA GCACCAACAA CAACTGCAAC GCGCGACAAC GCAACAGCAG CAACATCAGC 
AGCAACAACA CCATCAGCAG CAACAGCAGC AGCAGCAAAA GCACTTGCAA CAACAATAGC 
AAACAGCAAC ACGCCACGCC CATGCCGCCC CTAGCTAATG CCAACAAAGC CCCGTTAATT 
GTGCTTAAGA CAGCCGCATC TGCAGCAGCA TTGCCGTCCA TTGCCGCAGC AGCAACATCC 
AGCGGCACGG CAGCAACATC TGGTGGTGTT GGTTCGCCCA TCAAGCGTCG CAAGAATCCG 
CTGTCGCCGC TGCTCCTCGG CAGCCAGTGC CAGTCCACCA TTGCGCCTCC GCAGGCCATT 
GCCAGTCCGC TCCTGAGCGG CGGCAGCTGC TCCTCCTCGA CCACCTCGCC CCAATCCTCG 
CCAGCGGTTC GCACCACCAA GGCGAGTCGT CTGCGCGCCG CCGCGCTTGA TAAAAAGAAG 
GAAGAGGAGC AACGCAATCG CTACAGTTCG CCCGTTTCAC CAAAAGCCTC CAGCAACGGC 
GGAGTCCAAG CACAACGTCG TCTTAGCAAC TCGGAGCTAT CGCCCAAGTC GCCAGGTGCC 
AGTGGATTCA CTTCGGAGCC AGTCGCGTCA GCCACAGCGA ACAGAGTCCC GACGAGATCT 
GCCTCCCATC CTGTGGACAC ACCGCCCACA CAAAGGCGAC AGCCTCTGGT GGCCGTGAGT 
TCGGGCATCT CCATGAAGCA GAGTCTCAGC GAGCTGCACT TCACGGGTGG AGCAGTCAGT 
CCGCCCAAGG CTACGGCGGA ATGCACTCTA AGGATGCGAC CACGTAGCTC CACGATGAGC 
ACCGAGAACA CGGAGAATGG CCGACGTGAC CACTTGGCTA ATCTGCAACT AAGTCCTGTG 
CAAAAGCAAA AGCGAACGCC CGAAGTATCA CCACGCCGTT TCGGAGATCG CGACAAGTCG 
GCCAAACGGA AATCGAATTT GAATCGTTCG TTGACCGAGG AAGCCACCAA GGATGGCGAT 
GAGGTTGCCT CTTCGACCCG CCGGCGCCGA CGTCGTGAAC TGGGAATGGC TCGTCCCCTG 
GTTCCGGGCG ATGATGACGC CCTAAGAGAT TTGCTAACGC CGCAGAAGAG TTCCTGCAGC 
GAGTCAACCA ACTCCATATC CGAACCCCAT CTCCAGTTCA TACCAGCCGT TCGTCATCCG 
GTGGTTCCAG TACGCACCAT GACTGCTGTG CTGCCTGCAC CACGACCGCT GCATACTCCT 
GCGGCCCGCA ACGTCAGCCA GAAGTTCAAT GAAAGGACCA AAACAGATCT GGCCGAGATC 
AAGAAGATTG CTGAGCAATC GGAGGCGCAG GTGCAACAGC CTTCGGTGGA TGATAACCAG 
AAAATGTCAG CCATAATGCG CAGGAAGAGC ACGGATGAGG GTGGATCAGC TTTGGGGGCA 
ACAAACCAGA ATTCACCACC TGCCCCAAAC CAAATTGTAT CCATACTGAA GAAGAAGGAA 
CATGGACTTG GCGAGTGCAA CTCCAGTGCT TCAAGCAATC CCTCGCCGGT GACCTTCTCT 
TCTAGCGTGG TGGATCATCC TTTGGCTGGA GCTGGCCGGT GCAAACGGCA GGGAATACTC 
AAGAAGAGAT CGAGTCTAGA TGAATCGCGC TACTACTCCC GATCGCATTC ACCCGACGAA 
CGTAGTATTC TCATTAAGTC GGCCAGGAGG AATTCCCTGG AGGAAGCTGG CACTGGATTG 
AGTCCGGCTC AGGCCCATGG AATCCTCAAG CAGAGCAGCT ACGACAGCAG CAAGAGCGAC 
GGCTGCCCTT CGGCCACGGA GAGCCAGCCA CACAGTATCC TGAAAAAGAA GGATTCGCTA 
TCGACACCTT CGGACGGAGG ATGCCACAAG CATGTCTCTA TATCGCAAGC TGTAACTTTA 
GCTGCTGCTG AACTGGCTGC CCATGATGGA ATCACCTTCG ATGATGGTGA GGAGCATGAG 
ATACGACCGA TCCTAAAGCA GGAGAGCACC TCCAGCGAGG AGGCTGTGCG TCCGCCAAAG 
CCCATACTCA AAAAGAAATC CTTTGGCGAG GCGGATGAAC ACGAAATACG TCCTATATTG 
AAGAGCTCTC GTAAAAGCAG TCGAGAGGAG TTCGATTTGA GTGGCTTGGA AAACGAGGTC 
AACGATTCCC TATCGTCAAT CCTAAAGACA GACTCGCCGT CCAAGCGTCG GTCGCTCGGT 
TCAGCTTTGC ATGATTTGGA GGAGTCCACC ACTTCCCCGA GCATGCTCAA GAGGCGCACC 
CGTTCGCTGG AACGTCAAGA TGTCCAGCCT GGCATGGATT TGGCAGCTGC ACTGGATGCC 
ATCGCCACAT CACAGGCTAC TCCCAGCACC CCGGTGGACT TCGTGACCAC ACCAACAAGT 
ATATCGGTGG CGGAGCGGAT CAAGCGCATG GAGATGCTGT CGACGCCCAC TTCCCGTGCG 
GCGGGTGGGG CCAACAGCAG CTGGGAATCG CCACTGCAGG CATCGTCAGC AGAGCATGGA 
GCACCCACCA CCCCAAGGGT TCTCAAGCCA AGTGTGCTGC GCAGAGATTT GCAGAGGGAG 
CGGTACAAAA CGCAACCCGT GACCAACGAG GAGATGAGCT TCTTCAAAGC CAACTCCGCG 
CCCTTCGACA TCTCGGCTAC ATCGGCCTTC AAGCCCACCA AGCCGCTGGA CATCCGCTTG 
ACCCACGCAC CGCATGCACC GCTGATTTCG CCGATCACCG GACAGCCACT CAGCCACGTG 
CCCGCGCCAC TGCTGCTATC CTCGTCGACG AACTCGGGAG CGGGATCCTC GTTCCGTCTG 
GCCCGCAGCT CCACCCAACC GCTGCAGTCG CCGCGAGTTG TCCACCTGGC CGGTCAGGCT 
ACTGGTGGCT CCGCTTTGGC ACGCCAGAAC TCGGTGAACG ACAGCATCAA TCTCAGCGAG 
CAGCTAACCC TATCCATCGG CACGGACAGC GAGCACATCT TTGAGATGTC CGCTTTGGAG 
GAGGACTCGG CTATTCAGTC GCTGAGCGAC GAGACAGCCG CTTCCGCATC TGCCAACTCA 
ACATCCACGA CCACTGCAAC CACCATGACA CCGCCCAGTG GCCTGACGCG GAGCAACAGC 
GTTCGGGCCA GGGCCAATAT GTTCCAGCAA TTGCAGGAGC AGTCGCGCAA CCAACGGGCA 
ACGGGAACCA CCAACTCCCG TCAATCTCCA CCAGCGTCGT CGGCGGAGGT GTCTTCAAGT 
GCAGCCGACA ATTCCATGCA CAGTGACGAG CGGTCCACGC CAAATGCAAC GATAGATGCG 
GAATTCGGAA AACCCAGCAC CGAACCACTT AAGAGCATTC TTAAGACGGG AATGCCAGTG 
ATGGGTATGG GACGCGGTTT AATGCCTGTG GATCTCAATG CCGAGCTGAA GAACCGGCTC 
AAACGCTCCA CACATGCCAC TGTTTCCAAT CTCCGCAAAT CTGCCACCTC GGCAGATGCC 
ACTAGAGCAG CTAGTGATGA GGTGGACAAT CCGCAGAGGA ATTTAGCCCA GATCCTGAGG 
AATGTTTCCA AGGAGAACTC CACGCCGAAG CACGATGATG CCGTGAGCCT AGTGCGGAAT 
CTCTCTACTT TGGGACAACC TCGATCGCTG GCCCGTGACG ATGCAGCCGG GAATTGTGCC 
TCGGCATCCG AAGGCGAGAG TTCGGGGGGC CGCGAGATCG AGGCCATAAT CAAGAACAGC 
GCTGTGGCCA GACGTCGCCG GCAGCAACAG CAGCAGTCGC AGCAGCAGAA TCAGCCAGAT 
GGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Svil ( NM_001274399.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261328.1 Drosophila melanogaster supervillin, transcript variant U (Svil), mRNA.

Price & Availability↑

CloneID	OFa19999
Clone ID Related Accession (Same CDS sequence)	NM_001274399.1
Accession Version	NM_001274399.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	supervillin, isoform U
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCAGCG GCAACTCGGG CGGCAGCAGT GGCAATCCCA AGCAGCAGCA GCAGCAGCAG 
CAGCAACAAC AGCTAGAGCA GCTGGGACAC CAGACACATC TTGTGCTAGG AGCATCAAAG 
GAGTTGCCCG CGGTCAGCAG CAGCAGCGAT GAAGAGGGAT CCCTGCGGAC TCTCCACTCC 
AGTCGCAAGG TGACCAATCT GAATGCCAAG TCCACCTCTC TGCCACCCGA GGAAGGACTC 
TCGGGTGATG TCTCCCTGGA GTCGTCCTCC AACCGACTGA ATCTGCGATT GGCCAGGAGC 
CTGGAGCCCT CAGTCCGCAA TATCACTGCA TCCGCCTCCA AGGAAGTACA GAGCTATGAT 
GCTGGGGAGC CTGTGAATAC CAATCGATTG TTTGTGGAAC CGCCCAAGAT ACCACTGTCT 
GCCATCCGAA ACTCTCTTGA CGATCACGAC CTGGATATTG AGGAGTGCGA CAGCACCACC 
CAGTTCGATC AGGGGGATAT CTACGATACC CTGGAACGCT GGAGCCATGG TGTCAGCTTG 
GGGGCACATC TATTGAACTG CGCGAATGGC CACGATCAAT CTGATCCCGA GGACGACCAT 
CAAACCGATC ATCATCAGCA TGCACATCAA TCATTGCCAG TAGAACCCAA GTCTCATCAT 
CATCAGCACC ACCAGGCCAC CAATGCGCGC AGATTCATAA CGCGCACCAC GCGTTCCGCC 
AGTCGCCAGA GTTCCGCCGG ACACTCCCCA CCTCCGCCGC TGCATCGCAG TGACGACTCC 
TCCAGCAGTT CACCCTCACC GACACGCAGA CGGAATAAGC CAGGTGGTGC TCCTGCCGCC 
ACAGATCCAC CATCATCGTC GGCGAACGGG AATAGTTCCG CTTATGAGAC GGCAGCCACC 
ACTGTGATGA CCCATGACCA GCAGCGAGAT CCTCCAGTTG AAGTTCCTAA GGCTCTAAAC 
GTCGAGCCAC CGCAGCCAGA AGACATCGTC CTGGGCTCCT GCCCCACTGC ATCTGCCCAC 
TCAGATGAGG TTGCCTCTTC GACCCGCCGG CGCCGACGTC GTGAACTGGG AATGGCTCGT 
CCCCTGGTTC CGGGCGATGA TGACGCCCTA AGAGATTTGC TAACGCCGCA GAAGAGTTCC 
TGCAGCGAGT CAACCAACTC CATATCCGAA CCCCATCTCC AGTTCATACC AGCCGTTCGT 
CATCCGGTGG TTCCAGTACG CACCATGACT GCTGTGCTGC CTGCACCACG ACCGCTGCAT 
ACTCCTGCGG CCCGCAACGT CAGCCAGAAG TTCAATGAAA GGACCAAAAC AGATCTGGCC 
GAGATCAAGA AGATTGCTGA GCAATCGGAG GCGCAGGTGC AACAGCCTTC GGTGGATGAT 
AACCAGAAAA TGTCAGCCAT AATGCGCAGG AAGAGCACGG ATGAGGGTGG ATCAGCTTTG 
GGGGCAACAA ACCAGAATTC ACCACCTGCC CCAAACCAAA TTGTATCCAT ACTGAAGAAG 
AAGGAACATG GACTTGGCGA GTGCAACTCC AGTGCTTCAA GCAATCCCTC GCCGGTGACC 
TTCTCTTCTA GCGTGGTGGA TCATCCTTTG GCTGGAGCTG GCCGGTGCAA ACGGCAGGGA 
ATACTCAAGA AGAGATCGAG TCTAGATGAA TCGCGCTACT ACTCCCGATC GCATTCACCC 
GACGAACGTA GTATTCTCAT TAAGTCGGCC AGGAGGAATT CCCTGGAGGA AGCTGGCACT 
GGATTGAGTC CGGCTCAGGC CCATGGAATC CTCAAGCAGA GCAGCTACGA CAGCAGCAAG 
AGCGACGGCT GCCCTTCGGC CACGGAGAGC CAGCCACACA GTATCCTGAA AAAGAAGGAT 
TCGCTATCGA CACCTTCGGA CGGAGGATGC CACAAGCATG TCTCTATATC GCAAGCTGTA 
ACTTTAGCTG CTGCTGAACT GGCTGCCCAT GATGGAATCA CCTTCGATGA TGGTGAGGAG 
CATGAGATAC GACCGATCCT AAAGCAGGAG AGCACCTCCA GCGAGGAGGC TGTGCGTCCG 
CCAAAGCCCA TACTCAAAAA GAAATCCTTT GGCGAGGCGG ATGAACACGA AATACGTCCT 
ATATTGAAGA GCTCTCGTAA AAGCAGTCGA GAGGAGTTCG ATTTGAGTGG CTTGGAAAAC 
GAGGTCAACG ATTCCCTATC GTCAATCCTA AAGACAGACT CGCCGTCCAA GCGTCGGTCG 
CTCGGTTCAG CTTTGCATGA TTTGGAGGAG TCCACCACTT CCCCGAGCAT GCTCAAGAGG 
CGCACCCGTT CGCTGGAACG TCAAGATGTC CAGCCTGGCA TGGATTTGGC AGCTGCACTG 
GATGCCATCG CCACATCACA GGCTACTCCC AGCACCCCGG TGGACTTCGT GACCACACCA 
ACAAGTATAT CGGTGGCGGA GCGGATCAAG CGCATGGAGA TGCTGTCGAC GCCCACTTCC 
CGTGCGGCGG GTGGGGCCAA CAGCAGCTGG GAATCGCCAC TGCAGGCATC GTCAGCAGAG 
CATGGAGCAC CCACCACCCC AAGGGTTCTC AAGCCAAGTG TGCTGCGCAG AGATTTGCAG 
AGGGAGCGGT ACAAAACGCA ACCCGTGACC AACGAGGAGA TGAGCTTCTT CAAAGCCAAC 
TCCGCGCCCT TCGACATCTC GGCTACATCG GCCTTCAAGC CCACCAAGCC GCTGGACATC 
CGCTTGACCC ACGCACCGCA TGCACCGCTG ATTTCGCCGA TCACCGGACA GCCACTCAGC 
CACGTGCCCG CGCCACTGCT GCTATCCTCG TCGACGAACT CGGGAGCGGG ATCCTCGTTC 
CGTCTGGCCC GCAGCTCCAC CCAACCGCTG CAGTCGCCGC GAGTTGTCCA CCTGGCCGGT 
CAGGCTACTG GTGGCTCCGC TTTGGCACGC CAGAACTCGG TGAACGACAG CATCAATCTC 
AGCGAGCAGC TAACCCTATC CATCGGCACG GACAGCGAGC ACATCTTTGA GATGTCCGCT 
TTGGAGGAGG ACTCGGCTAT TCAGTCGCTG AGCGACGAGA CAGCCGCTTC CGCATCTGCC 
AACTCAACAT CCACGACCAC TGCAACCACC ATGACACCGC CCAGTGGCCT GACGCGGAGC 
AACAGCGTTC GGGCCAGGGC CAATATGTTC CAGCAATTGC AGGAGCAGTC GCGCAACCAA 
CGGGCAACGG GAACCACCAA CTCCCGTCAA TCTCCACCAG CGTCGTCGGC GGAGGTGTCT 
TCAAGTGCAG CCGACAATTC CATGCACAGT GACGAGCGGT CCACGCCAAA TGCAACGATA 
GATGCGGAAT TCGAACCCTC ATCCCTGTCG CTGGCTGAGC GACTGGCCTT CTTCAGCAAT 
CTGTGCGAGA GCGGCGGAGG AGGTGGGAGT ACCAGTGCCG GTGGAAGGTA CCGCAGTCGC 
ACCAGCAGCT ATTCAAGGAG TCCGCCCGGC GATCGCACCA CTCCCAGGAG CAGCACCACC 
GCCAGCAGTG TCAGTATGAC GCCCACGCCC ATGGATTACT CTCCGATTCC CCACGACAAG 
GAGCCATCTA GTCTCACGCT GGAGAGCATC ATCGAGCCCG AGCAGGAGGA GGTCAAGGAG 
CAGGTGGACC AGGATGAGGT CAAGTTGAGG AAGAAGGAGC CAGTCCAGAG GGAATCTTCC 
CAGCACAATG GCTTCCACGT GCTGACCAGG TCCGCCACCG AACCGCCGTC GTCCACCTCG 
CCCCTGCGCA TCAGCATACG GACTGTGGGA AAGCTAGTCC TGCCCGATAC CTTCCGCGGC 
GATCGCAACA ATAACAGCAG TAGTAGAAGT GGGAGCAACA GCTGGAGCAA CACTTGCATG 
GTCAAGCTGC AGAGCTTGGA GCCCGTTAGC CTGTCCGTTC ATTCGCGCAC CATTGGTAAG 
GTTAAGTCGC CATTCATTGA AAAGCAGCAG GAGAAACCAC ACACCGAGAA GCTAGCGAAC 
GGAACCAGCG ACAGTGGCTC CGATTCGGGC AAGGAGAACT GCGCCTCCAA CGAGCAGGAA 
CATCTGCAGC ACCAGCAGTC GCAGGACGAG GATCGAGCTC CGCCCAGGGT GTCCGAGATG 
CGGAGGAAAA TCAGCCGACT GGTGCAACAA CAGCAGCCGC AACCACAGCC CGTCAATCGG 
CGGCACACCA CCGAGATCAC CTCCGTGACC GTTGGAACGC GTTCGCCCAG TGTGGACGAT 
CGCTTTGCCA AGTACTTTGG TGTTAAGGAG AGCTGCAACA GCACGACCAC CGTGCATAAA 
ACGCAATCGC TGCCACCCAG CCAAGTTGAG GCCACCAATG CGCACGTGAA ACTACCGCAG 
ATCACTACCG TGGTTTCCAA ACGGCGCGAG CTGCTCAAGA GGACGCGACC CCTGTCCATG 
GATCACTACT CCAGCGGCTG CACCAGTCCC ACAGCCATGC CAAGTCCCAA GAGAGCACAT 
TCGCCCGTCG GCCGGCGGAA GGCCGCCATT TCCAGCATCG AGGACATTGA GGTCACGCCG 
GATGAACTCC GGGCCGCTGG CAGGAACTTC AAGCTGTTGT ACGGCGAGTT ATTGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261328.1
CDS	1293..5852
Translation	MSSGNSGGSSGNPKQQQQQQQQQQLEQLGHQTHLVLGASKELPAVSSSSDEEGSLRTLHSSRKVTNLNAKSTSLPPEEGLSGDVSLESSSNRLNLRLARSLEPSVRNITASASKEVQSYDAGEPVNTNRLFVEPPKIPLSAIRNSLDDHDLDIEECDSTTQFDQGDIYDTLERWSHGVSLGAHLLNCANGHDQSDPEDDHQTDHHQHAHQSLPVEPKSHHHQHHQATNARRFITRTTRSASRQSSAGHSPPPPLHRSDDSSSSSPSPTRRRNKPGGAPAATDPPSSSANGNSSAYETAATTVMTHDQQRDPPVEVPKALNVEPPQPEDIVLGSCPTASAHSDEVASSTRRRRRRELGMARPLVPGDDDALRDLLTPQKSSCSESTNSISEPHLQFIPAVRHPVVPVRTMTAVLPAPRPLHTPAARNVSQKFNERTKTDLAEIKKIAEQSEAQVQQPSVDDNQKMSAIMRRKSTDEGGSALGATNQNSPPAPNQIVSILKKKEHGLGECNSSASSNPSPVTFSSSVVDHPLAGAGRCKRQGILKKRSSLDESRYYSRSHSPDERSILIKSARRNSLEEAGTGLSPAQAHGILKQSSYDSSKSDGCPSATESQPHSILKKKDSLSTPSDGGCHKHVSISQAVTLAAAELAAHDGITFDDGEEHEIRPILKQESTSSEEAVRPPKPILKKKSFGEADEHEIRPILKSSRKSSREEFDLSGLENEVNDSLSSILKTDSPSKRRSLGSALHDLEESTTSPSMLKRRTRSLERQDVQPGMDLAAALDAIATSQATPSTPVDFVTTPTSISVAERIKRMEMLSTPTSRAAGGANSSWESPLQASSAEHGAPTTPRVLKPSVLRRDLQRERYKTQPVTNEEMSFFKANSAPFDISATSAFKPTKPLDIRLTHAPHAPLISPITGQPLSHVPAPLLLSSSTNSGAGSSFRLARSSTQPLQSPRVVHLAGQATGGSALARQNSVNDSINLSEQLTLSIGTDSEHIFEMSALEEDSAIQSLSDETAASASANSTSTTTATTMTPPSGLTRSNSVRARANMFQQLQEQSRNQRATGTTNSRQSPPASSAEVSSSAADNSMHSDERSTPNATIDAEFEPSSLSLAERLAFFSNLCESGGGGGSTSAGGRYRSRTSSYSRSPPGDRTTPRSSTTASSVSMTPTPMDYSPIPHDKEPSSLTLESIIEPEQEEVKEQVDQDEVKLRKKEPVQRESSQHNGFHVLTRSATEPPSSTSPLRISIRTVGKLVLPDTFRGDRNNNSSSRSGSNSWSNTCMVKLQSLEPVSLSVHSRTIGKVKSPFIEKQQEKPHTEKLANGTSDSGSDSGKENCASNEQEHLQHQQSQDEDRAPPRVSEMRRKISRLVQQQQPQPQPVNRRHTTEITSVTVGTRSPSVDDRFAKYFGVKESCNSTTTVHKTQSLPPSQVEATNAHVKLPQITTVVSKRRELLKRTRPLSMDHYSSGCTSPTAMPSPKRAHSPVGRRKAAISSIEDIEVTPDELRAAGRNFKLLYGELLG

Target ORF information:

RefSeq Version	NM_001274399.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster supervillin, transcript variant U (Svil), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274399.1

ORF Insert Sequence:

ATGAGCAGCG GCAACTCGGG CGGCAGCAGT GGCAATCCCA AGCAGCAGCA GCAGCAGCAG 
CAGCAACAAC AGCTAGAGCA GCTGGGACAC CAGACACATC TTGTGCTAGG AGCATCAAAG 
GAGTTGCCCG CGGTCAGCAG CAGCAGCGAT GAAGAGGGAT CCCTGCGGAC TCTCCACTCC 
AGTCGCAAGG TGACCAATCT GAATGCCAAG TCCACCTCTC TGCCACCCGA GGAAGGACTC 
TCGGGTGATG TCTCCCTGGA GTCGTCCTCC AACCGACTGA ATCTGCGATT GGCCAGGAGC 
CTGGAGCCCT CAGTCCGCAA TATCACTGCA TCCGCCTCCA AGGAAGTACA GAGCTATGAT 
GCTGGGGAGC CTGTGAATAC CAATCGATTG TTTGTGGAAC CGCCCAAGAT ACCACTGTCT 
GCCATCCGAA ACTCTCTTGA CGATCACGAC CTGGATATTG AGGAGTGCGA CAGCACCACC 
CAGTTCGATC AGGGGGATAT CTACGATACC CTGGAACGCT GGAGCCATGG TGTCAGCTTG 
GGGGCACATC TATTGAACTG CGCGAATGGC CACGATCAAT CTGATCCCGA GGACGACCAT 
CAAACCGATC ATCATCAGCA TGCACATCAA TCATTGCCAG TAGAACCCAA GTCTCATCAT 
CATCAGCACC ACCAGGCCAC CAATGCGCGC AGATTCATAA CGCGCACCAC GCGTTCCGCC 
AGTCGCCAGA GTTCCGCCGG ACACTCCCCA CCTCCGCCGC TGCATCGCAG TGACGACTCC 
TCCAGCAGTT CACCCTCACC GACACGCAGA CGGAATAAGC CAGGTGGTGC TCCTGCCGCC 
ACAGATCCAC CATCATCGTC GGCGAACGGG AATAGTTCCG CTTATGAGAC GGCAGCCACC 
ACTGTGATGA CCCATGACCA GCAGCGAGAT CCTCCAGTTG AAGTTCCTAA GGCTCTAAAC 
GTCGAGCCAC CGCAGCCAGA AGACATCGTC CTGGGCTCCT GCCCCACTGC ATCTGCCCAC 
TCAGATGAGG TTGCCTCTTC GACCCGCCGG CGCCGACGTC GTGAACTGGG AATGGCTCGT 
CCCCTGGTTC CGGGCGATGA TGACGCCCTA AGAGATTTGC TAACGCCGCA GAAGAGTTCC 
TGCAGCGAGT CAACCAACTC CATATCCGAA CCCCATCTCC AGTTCATACC AGCCGTTCGT 
CATCCGGTGG TTCCAGTACG CACCATGACT GCTGTGCTGC CTGCACCACG ACCGCTGCAT 
ACTCCTGCGG CCCGCAACGT CAGCCAGAAG TTCAATGAAA GGACCAAAAC AGATCTGGCC 
GAGATCAAGA AGATTGCTGA GCAATCGGAG GCGCAGGTGC AACAGCCTTC GGTGGATGAT 
AACCAGAAAA TGTCAGCCAT AATGCGCAGG AAGAGCACGG ATGAGGGTGG ATCAGCTTTG 
GGGGCAACAA ACCAGAATTC ACCACCTGCC CCAAACCAAA TTGTATCCAT ACTGAAGAAG 
AAGGAACATG GACTTGGCGA GTGCAACTCC AGTGCTTCAA GCAATCCCTC GCCGGTGACC 
TTCTCTTCTA GCGTGGTGGA TCATCCTTTG GCTGGAGCTG GCCGGTGCAA ACGGCAGGGA 
ATACTCAAGA AGAGATCGAG TCTAGATGAA TCGCGCTACT ACTCCCGATC GCATTCACCC 
GACGAACGTA GTATTCTCAT TAAGTCGGCC AGGAGGAATT CCCTGGAGGA AGCTGGCACT 
GGATTGAGTC CGGCTCAGGC CCATGGAATC CTCAAGCAGA GCAGCTACGA CAGCAGCAAG 
AGCGACGGCT GCCCTTCGGC CACGGAGAGC CAGCCACACA GTATCCTGAA AAAGAAGGAT 
TCGCTATCGA CACCTTCGGA CGGAGGATGC CACAAGCATG TCTCTATATC GCAAGCTGTA 
ACTTTAGCTG CTGCTGAACT GGCTGCCCAT GATGGAATCA CCTTCGATGA TGGTGAGGAG 
CATGAGATAC GACCGATCCT AAAGCAGGAG AGCACCTCCA GCGAGGAGGC TGTGCGTCCG 
CCAAAGCCCA TACTCAAAAA GAAATCCTTT GGCGAGGCGG ATGAACACGA AATACGTCCT 
ATATTGAAGA GCTCTCGTAA AAGCAGTCGA GAGGAGTTCG ATTTGAGTGG CTTGGAAAAC 
GAGGTCAACG ATTCCCTATC GTCAATCCTA AAGACAGACT CGCCGTCCAA GCGTCGGTCG 
CTCGGTTCAG CTTTGCATGA TTTGGAGGAG TCCACCACTT CCCCGAGCAT GCTCAAGAGG 
CGCACCCGTT CGCTGGAACG TCAAGATGTC CAGCCTGGCA TGGATTTGGC AGCTGCACTG 
GATGCCATCG CCACATCACA GGCTACTCCC AGCACCCCGG TGGACTTCGT GACCACACCA 
ACAAGTATAT CGGTGGCGGA GCGGATCAAG CGCATGGAGA TGCTGTCGAC GCCCACTTCC 
CGTGCGGCGG GTGGGGCCAA CAGCAGCTGG GAATCGCCAC TGCAGGCATC GTCAGCAGAG 
CATGGAGCAC CCACCACCCC AAGGGTTCTC AAGCCAAGTG TGCTGCGCAG AGATTTGCAG 
AGGGAGCGGT ACAAAACGCA ACCCGTGACC AACGAGGAGA TGAGCTTCTT CAAAGCCAAC 
TCCGCGCCCT TCGACATCTC GGCTACATCG GCCTTCAAGC CCACCAAGCC GCTGGACATC 
CGCTTGACCC ACGCACCGCA TGCACCGCTG ATTTCGCCGA TCACCGGACA GCCACTCAGC 
CACGTGCCCG CGCCACTGCT GCTATCCTCG TCGACGAACT CGGGAGCGGG ATCCTCGTTC 
CGTCTGGCCC GCAGCTCCAC CCAACCGCTG CAGTCGCCGC GAGTTGTCCA CCTGGCCGGT 
CAGGCTACTG GTGGCTCCGC TTTGGCACGC CAGAACTCGG TGAACGACAG CATCAATCTC 
AGCGAGCAGC TAACCCTATC CATCGGCACG GACAGCGAGC ACATCTTTGA GATGTCCGCT 
TTGGAGGAGG ACTCGGCTAT TCAGTCGCTG AGCGACGAGA CAGCCGCTTC CGCATCTGCC 
AACTCAACAT CCACGACCAC TGCAACCACC ATGACACCGC CCAGTGGCCT GACGCGGAGC 
AACAGCGTTC GGGCCAGGGC CAATATGTTC CAGCAATTGC AGGAGCAGTC GCGCAACCAA 
CGGGCAACGG GAACCACCAA CTCCCGTCAA TCTCCACCAG CGTCGTCGGC GGAGGTGTCT 
TCAAGTGCAG CCGACAATTC CATGCACAGT GACGAGCGGT CCACGCCAAA TGCAACGATA 
GATGCGGAAT TCGAACCCTC ATCCCTGTCG CTGGCTGAGC GACTGGCCTT CTTCAGCAAT 
CTGTGCGAGA GCGGCGGAGG AGGTGGGAGT ACCAGTGCCG GTGGAAGGTA CCGCAGTCGC 
ACCAGCAGCT ATTCAAGGAG TCCGCCCGGC GATCGCACCA CTCCCAGGAG CAGCACCACC 
GCCAGCAGTG TCAGTATGAC GCCCACGCCC ATGGATTACT CTCCGATTCC CCACGACAAG 
GAGCCATCTA GTCTCACGCT GGAGAGCATC ATCGAGCCCG AGCAGGAGGA GGTCAAGGAG 
CAGGTGGACC AGGATGAGGT CAAGTTGAGG AAGAAGGAGC CAGTCCAGAG GGAATCTTCC 
CAGCACAATG GCTTCCACGT GCTGACCAGG TCCGCCACCG AACCGCCGTC GTCCACCTCG 
CCCCTGCGCA TCAGCATACG GACTGTGGGA AAGCTAGTCC TGCCCGATAC CTTCCGCGGC 
GATCGCAACA ATAACAGCAG TAGTAGAAGT GGGAGCAACA GCTGGAGCAA CACTTGCATG 
GTCAAGCTGC AGAGCTTGGA GCCCGTTAGC CTGTCCGTTC ATTCGCGCAC CATTGGTAAG 
GTTAAGTCGC CATTCATTGA AAAGCAGCAG GAGAAACCAC ACACCGAGAA GCTAGCGAAC 
GGAACCAGCG ACAGTGGCTC CGATTCGGGC AAGGAGAACT GCGCCTCCAA CGAGCAGGAA 
CATCTGCAGC ACCAGCAGTC GCAGGACGAG GATCGAGCTC CGCCCAGGGT GTCCGAGATG 
CGGAGGAAAA TCAGCCGACT GGTGCAACAA CAGCAGCCGC AACCACAGCC CGTCAATCGG 
CGGCACACCA CCGAGATCAC CTCCGTGACC GTTGGAACGC GTTCGCCCAG TGTGGACGAT 
CGCTTTGCCA AGTACTTTGG TGTTAAGGAG AGCTGCAACA GCACGACCAC CGTGCATAAA 
ACGCAATCGC TGCCACCCAG CCAAGTTGAG GCCACCAATG CGCACGTGAA ACTACCGCAG 
ATCACTACCG TGGTTTCCAA ACGGCGCGAG CTGCTCAAGA GGACGCGACC CCTGTCCATG 
GATCACTACT CCAGCGGCTG CACCAGTCCC ACAGCCATGC CAAGTCCCAA GAGAGCACAT 
TCGCCCGTCG GCCGGCGGAA GGCCGCCATT TCCAGCATCG AGGACATTGA GGTCACGCCG 
GATGAACTCC GGGCCGCTGG CAGGAACTTC AAGCTGTTGT ACGGCGAGTT ATTGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Svil ( NM_001202102.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001189031.1 Drosophila melanogaster supervillin, transcript variant G (Svil), mRNA.

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CloneID	OFa20000
Clone ID Related Accession (Same CDS sequence)	NM_001202102.2
Accession Version	NM_001202102.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5904bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	supervillin, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001202102.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGGCCC TCAAGGAGTG CCTCATACGG GAGGGTGTTC TCAATCTGGA CAGCCCCGAG 
AAGTACCGAC CGTACCATCT AAACGGCAGC AAAAGCAGCG GCGCCATGGA TTTTCCCCGA 
TATCGCAAAA CCATTGCGGC CTCAAGTGAC TTATCCACAG CACCTGCCAC TGCCAAGACC 
AGCTGCCAGC CACCTGAGCC ACTGAAACAG AAGCCACCGC AGCAGCAGAC CACCAAGGAC 
TCGGAGGATT CCAGTTCACA GAAGAGTGCT CCAGTCCAGT CGTATCGTGG TCAGCATCCG 
CACTTCCACC ATCACACCAC GCCCGCTTAC TACACGGATC TTCAGCTGAG AATTCACACG 
CCGAACGCAT CGGATGTGGG CGCCTTGAAC GAGAACTATC GAAGTGTATA CAGTACTCCG 
AGCACCCAGC TGGTGGAACA GCGATCGTTT CCAAAGAGCT CCACGGCCAC GAAGGATCCA 
CCGCTGCCCA TAAGCAATCC GGCTAGGGGA ACATTTTTTC GTGGCGACTC TGCGTCCAGC 
GACAGTCACT CAAGACTCCG TCGGAGCCAG CAGAGAACCG GCAGCGAGGG ACGCTTCTTC 
AGATACTCTC AGGGATCTGT GAGCCCTACC AAAGGTGAAC CTCCAATCGG CCACGAAGGT 
GGTGAGCAGC CACCCGTTGT CGCCAGTCAC TCCCCCATCA CATCCACCAG CACCACCACC 
ATCAGTAGTT GTAACCCCGT AGAGCAGGCT AGTCCACCAC CAACAAACGT AGTAAATCCC 
AGCGGATCCG GTCGCAACAG CCGGCACAAG CAGCGTTTCC ACAGTCGATC CCGTCGAGCG 
GTGCGCGTGC GCAGTGAATC GCGTCCGATT AGCGCCCTCT ATGACATAAT ATGCAAGGAG 
AAGAACCTGG AAAACAGCAG CAGCAGCAGC AGCAGCAGCA ACGAGCAGGA GAAGAAAAAG 
GATCAGGAGC AGCCTAAGAC CACAACCGAC AAGCTGGCGA CCTTTTGGCC CCTGCACCAT 
CACTACCTCA ATCCCCCGAT GAGCAGCGGC AACTCGGGCG GCAGCAGTGG CAATCCCAAG 
CAGCAGCAGC AGCAGCAGCA GCAACAACAG CTAGAGCAGC TGGGACACCA GACACATCTT 
GTGCTAGGAG CATCAAAGGA GTTGCCCGCG GTCAGCAGCA GCAGCGATGA AGAGGGATCC 
CTGCGGACTC TCCACTCCAG TCGCAAGGTG ACCAATCTGA ATGCCAAGTC CACCTCTCTG 
CCACCCGAGG AAGGACTCTC GGGTGATGTC TCCCTGGAGT CGTCCTCCAA CCGACTGAAT 
CTGCGATTGG CCAGGAGCCT GGAGCCCTCA GTCCGCAATA TCACTGCATC CGCCTCCAAG 
GAAGTACAGA GCTATGATGC TGGGGAGCCT GTGAATACCA ATCGATTGTT TGTGGAACCG 
CCCAAGATAC CACTGTCTGC CATCCGAAAC TCTCTTGACG ATCACGACCT GGATATTGAG 
GAGTGCGACA GCACCACCCA GTTCGATCAG GGGGATATCT ACGATACCCT GGAACGCTGG 
AGCCATGGTG TCAGCTTGGG GGCACATCTA TTGAACTGCG CGAATGGCCA CGATCAATCT 
GATCCCGAGG ACGACCATCA AACCGATCAT CATCAGCATG CACATCAATC ATTGCCAGTA 
GAACCCAAGT CTCATCATCA TCAGCACCAC CAGGCCACCA ATGCGCGCAG ATTCATAACG 
CGCACCACGC GTTCCGCCAG TCGCCAGAGT TCCGCCGGAC ACTCCCCACC TCCGCCGCTG 
CATCGCAGTG ACGACTCCTC CAGCAGTTCA CCCTCACCGA CACGCAGACG GAATAAGCCA 
GGTGGTGCTC CTGCCGCCAC AGATCCACCA TCATCGTCGG CGAACGGGAA TAGTTCCGCT 
TATGAGACGG CAGCCACCAC TGTGATGACC CATGACCAGC AGCGAGATCC TCCAGTTGAA 
GTTCCTAAGG CTCTAAACGT CGAGCCACCG CAGCCAGAAG ACATCGTCCT GGGCTCCTGC 
CCCACTGCAT CTGCCCACTC AGCAGAGAGC CAAAGTCGAG GAAAAGCTGG CAAATGCAGT 
ATTAATTGCA CCTACAGCCA CAGCCACAGC ACCAACAACA ACTGCAACGC GCGACAACGC 
AACAGCAGCA ACATCAGCAG CAACAACACC ATCAGCAGCA ACAGCAGCAG CAGCAAAAGC 
ACTTGCAACA ACAATAGCAA ACAGCAACAC GCCACGCCCA TGCCGCCCCT AGCTAATGCC 
AACAAAGCCC CGTTAATTGT GCTTAAGACA GCCGCATCTG CAGCAGCATT GCCGTCCATT 
GCCGCAGCAG CAACATCCAG CGGCACGGCA GCAACATCTG GTGGTGTTGG TTCGCCCATC 
AAGCGTCGCA AGAATCCGCT GTCGCCGCTG CTCCTCGGCA GCCAGTGCCA GTCCACCATT 
GCGCCTCCGC AGGCCATTGC CAGTCCGCTC CTGAGCGGCG GCAGCTGCTC CTCCTCGACC 
ACCTCGCCCC AATCCTCGCC AGCGGTTCGC ACCACCAAGG CGAGTCGTCT GCGCGCCGCC 
GCGCTTGATA AAAAGAAGGA AGAGGAGCAA CGCAATCGCT ACAGTTCGCC CGTTTCACCA 
AAAGCCTCCA GCAACGGCGG AGTCCAAGCA CAACGTCGTC TTAGCAACTC GGAGCTATCG 
CCCAAGTCGC CAGGTGCCAG TGGATTCACT TCGGAGCCAG TCGCGTCAGC CACAGCGAAC 
AGAGTCCCGA CGAGATCTGC CTCCCATCCT GTGGACACAC CGCCCACACA AAGGCGACAG 
CCTCTGGTGG CCGTGAGTTC GGGCATCTCC ATGAAGCAGA GTCTCAGCGA GCTGCACTTC 
ACGGGTGGAG CAGTCAGTCC GCCCAAGGCT ACGGCGGAAT GCACTCTAAG GATGCGACCA 
CGTAGCTCCA CGATGAGCAC CGAGAACACG GAGAATGGCC GACGTGACCA CTTGGCTAAT 
CTGCAACTAA GTCCTGTGCA AAAGCAAAAG CGAACGCCCG AAGTATCACC ACGCCGTTTC 
GGAGATCGCG ACAAGTCGGC CAAACGGAAA TCGAATTTGA ATCGTTCGTT GACCGAGGAA 
GCCACCAAGG ATGGCGATGA GGTTGCCTCT TCGACCCGCC GGCGCCGACG TCGTGAACTG 
GGAATGGCTC GTCCCCTGGT TCCGGGCGAT GATGACGCCC TAAGAGATTT GCTAACGCCG 
CAGAAGAGTT CCTGCAGCGA GTCAACCAAC TCCATATCCG AACCCCATCT CCAGTTCATA 
CCAGCCGTTC GTCATCCGGT GGTTCCAGTA CGCACCATGA CTGCTGTGCT GCCTGCACCA 
CGACCGCTGC ATACTCCTGC GGCCCGCAAC GTCAGCCAGA AGTTCAATGA AAGGACCAAA 
ACAGATCTGG CCGAGATCAA GAAGATTGCT GAGCAATCGG AGGCGCAGGT GCAACAGCCT 
TCGGTGGATG ATAACCAGAA AATGTCAGCC ATAATGCGCA GGAAGAGCAC GGATGAGGGT 
GGATCAGCTT TGGGGGCAAC AAACCAGAAT TCACCACCTG CCCCAAACCA AATTGTATCC 
ATACTGAAGA AGAAGGAACA TGGACTTGGC GAGTGCAACT CCAGTGCTTC AAGCAATCCC 
TCGCCGGTGA CCTTCTCTTC TAGCGTGGTG GATCATCCTT TGGCTGGAGC TGGCCGGTGC 
AAACGGCAGG GAATACTCAA GAAGAGATCG AGTCTAGATG AATCGCGCTA CTACTCCCGA 
TCGCATTCAC CCGACGAACG TAGTATTCTC ATTAAGTCGG CCAGGAGGAA TTCCCTGGAG 
GAAGCTGGCA CTGGATTGAG TCCGGCTCAG GCCCATGGAA TCCTCAAGCA GAGCAGCTAC 
GACAGCAGCA AGAGCGACGG CTGCCCTTCG GCCACGGAGA GCCAGCCACA CAGTATCCTG 
AAAAAGAAGG ATTCGCTATC GACACCTTCG GACGGAGGAT GCCACAAGCA TGTCTCTATA 
TCGCAAGCTG TAACTTTAGC TGCTGCTGAA CTGGCTGCCC ATGATGGAAT CACCTTCGAT 
GATGGTGAGG AGCATGAGAT ACGACCGATC CTAAAGCAGG AGAGCACCTC CAGCGAGGAG 
GCTGTGCGTC CGCCAAAGCC CATACTCAAA AAGAAATCCT TTGGCGAGGC GGATGAACAC 
GAAATACGTC CTATATTGAA GAGCTCTCGT AAAAGCAGTC GAGAGGAGTT CGATTTGAGT 
GGCTTGGAAA ACGAGGTCAA CGATTCCCTA TCGTCAATCC TAAAGACAGA CTCGCCGTCC 
AAGCGTCGGT CGCTCGGTTC AGCTTTGCAT GATTTGGAGG AGTCCACCAC TTCCCCGAGC 
ATGCTCAAGA GGCGCACCCG TTCGCTGGAA CGTCAAGATG TCCAGCCTGG CATGGATTTG 
GCAGCTGCAC TGGATGCCAT CGCCACATCA CAGGCTACTC CCAGCACCCC GGTGGACTTC 
GTGACCACAC CAACAAGTAT ATCGGTGGCG GAGCGGATCA AGCGCATGGA GATGCTGTCG 
ACGCCCACTT CCCGTGCGGC GGGTGGGGCC AACAGCAGCT GGGAATCGCC ACTGCAGGCA 
TCGTCAGCAG AGCATGGAGC ACCCACCACC CCAAGGGTTC TCAAGCCAAG TGTGCTGCGC 
AGAGATTTGC AGAGGGAGCG GTACAAAACG CAACCCGTGA CCAACGAGGA GATGAGCTTC 
TTCAAAGCCA ACTCCGCGCC CTTCGACATC TCGGCTACAT CGGCCTTCAA GCCCACCAAG 
CCGCTGGACA TCCGCTTGAC CCACGCACCG CATGCACCGC TGATTTCGCC GATCACCGGA 
CAGCCACTCA GCCACGTGCC CGCGCCACTG CTGCTATCCT CGTCGACGAA CTCGGGAGCG 
GGATCCTCGT TCCGTCTGGC CCGCAGCTCC ACCCAACCGC TGCAGTCGCC GCGAGTTGTC 
CACCTGGCCG GTCAGGCTAC TGGTGGCTCC GCTTTGGCAC GCCAGAACTC GGTGAACGAC 
AGCATCAATC TCAGCGAGCA GCTAACCCTA TCCATCGGCA CGGACAGCGA GCACATCTTT 
GAGATGTCCG CTTTGGAGGA GGACTCGGCT ATTCAGTCGC TGAGCGACGA GACAGCCGCT 
TCCGCATCTG CCAACTCAAC ATCCACGACC ACTGCAACCA CCATGACACC GCCCAGTGGC 
CTGACGCGGA GCAACAGCGT TCGGGCCAGG GCCAATATGT TCCAGCAATT GCAGGAGCAG 
TCGCGCAACC AACGGGCAAC GGGAACCACC AACTCCCGTC AATCTCCACC AGCGTCGTCG 
GCGGAGGTGT CTTCAAGTGC AGCCGACAAT TCCATGCACA GTGACGAGCG GTCCACGCCA 
AATGCAACGA TAGATGCGGA ATTCGGAAAA CCCAGCACCG AACCACTTAA GAGCATTCTT 
AAGACGGGAA TGCCAGTGAT GGGTATGGGA CGCGGTTTAA TGCCTGTGGA TCTCAATGCC 
GAGCTGAAGA ACCGGCTCAA ACGCTCCACA CATGCCACTG TTTCCAATCT CCGCAAATCT 
GCCACCTCGG CAGATGCCAC TAGAGCAGCT AGTGATGAGG TGGACAATCC GCAGAGGAAT 
TTAGCCCAGA TCCTGAGGAA TGTTTCCAAG GAGAACTCCA CGCCGAAGCA CGATGATGCC 
GTGAGCCTAG TGCGGAATCT CTCTACTTTG GGACAACCTC GATCGCTGGC CCGTGACGAT 
GCAGCCGGGA ATTGTGCCTC GGCATCCGAA GGCGAGAGTT CGGGGGGCCG CGAGATCGAG 
GCCATAATCA AGAACAGCGC TGTGGCCAGA CGTCGCCGGC AGCAACAGCA GCAGTCGCAG 
CAGCAGAATC AGCCAGATGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189031.1
CDS	394..6297
Translation	MLALKECLIREGVLNLDSPEKYRPYHLNGSKSSGAMDFPRYRKTIAASSDLSTAPATAKTSCQPPEPLKQKPPQQQTTKDSEDSSSQKSAPVQSYRGQHPHFHHHTTPAYYTDLQLRIHTPNASDVGALNENYRSVYSTPSTQLVEQRSFPKSSTATKDPPLPISNPARGTFFRGDSASSDSHSRLRRSQQRTGSEGRFFRYSQGSVSPTKGEPPIGHEGGEQPPVVASHSPITSTSTTTISSCNPVEQASPPPTNVVNPSGSGRNSRHKQRFHSRSRRAVRVRSESRPISALYDIICKEKNLENSSSSSSSSNEQEKKKDQEQPKTTTDKLATFWPLHHHYLNPPMSSGNSGGSSGNPKQQQQQQQQQQLEQLGHQTHLVLGASKELPAVSSSSDEEGSLRTLHSSRKVTNLNAKSTSLPPEEGLSGDVSLESSSNRLNLRLARSLEPSVRNITASASKEVQSYDAGEPVNTNRLFVEPPKIPLSAIRNSLDDHDLDIEECDSTTQFDQGDIYDTLERWSHGVSLGAHLLNCANGHDQSDPEDDHQTDHHQHAHQSLPVEPKSHHHQHHQATNARRFITRTTRSASRQSSAGHSPPPPLHRSDDSSSSSPSPTRRRNKPGGAPAATDPPSSSANGNSSAYETAATTVMTHDQQRDPPVEVPKALNVEPPQPEDIVLGSCPTASAHSAESQSRGKAGKCSINCTYSHSHSTNNNCNARQRNSSNISSNNTISSNSSSSKSTCNNNSKQQHATPMPPLANANKAPLIVLKTAASAAALPSIAAAATSSGTAATSGGVGSPIKRRKNPLSPLLLGSQCQSTIAPPQAIASPLLSGGSCSSSTTSPQSSPAVRTTKASRLRAAALDKKKEEEQRNRYSSPVSPKASSNGGVQAQRRLSNSELSPKSPGASGFTSEPVASATANRVPTRSASHPVDTPPTQRRQPLVAVSSGISMKQSLSELHFTGGAVSPPKATAECTLRMRPRSSTMSTENTENGRRDHLANLQLSPVQKQKRTPEVSPRRFGDRDKSAKRKSNLNRSLTEEATKDGDEVASSTRRRRRRELGMARPLVPGDDDALRDLLTPQKSSCSESTNSISEPHLQFIPAVRHPVVPVRTMTAVLPAPRPLHTPAARNVSQKFNERTKTDLAEIKKIAEQSEAQVQQPSVDDNQKMSAIMRRKSTDEGGSALGATNQNSPPAPNQIVSILKKKEHGLGECNSSASSNPSPVTFSSSVVDHPLAGAGRCKRQGILKKRSSLDESRYYSRSHSPDERSILIKSARRNSLEEAGTGLSPAQAHGILKQSSYDSSKSDGCPSATESQPHSILKKKDSLSTPSDGGCHKHVSISQAVTLAAAELAAHDGITFDDGEEHEIRPILKQESTSSEEAVRPPKPILKKKSFGEADEHEIRPILKSSRKSSREEFDLSGLENEVNDSLSSILKTDSPSKRRSLGSALHDLEESTTSPSMLKRRTRSLERQDVQPGMDLAAALDAIATSQATPSTPVDFVTTPTSISVAERIKRMEMLSTPTSRAAGGANSSWESPLQASSAEHGAPTTPRVLKPSVLRRDLQRERYKTQPVTNEEMSFFKANSAPFDISATSAFKPTKPLDIRLTHAPHAPLISPITGQPLSHVPAPLLLSSSTNSGAGSSFRLARSSTQPLQSPRVVHLAGQATGGSALARQNSVNDSINLSEQLTLSIGTDSEHIFEMSALEEDSAIQSLSDETAASASANSTSTTTATTMTPPSGLTRSNSVRARANMFQQLQEQSRNQRATGTTNSRQSPPASSAEVSSSAADNSMHSDERSTPNATIDAEFGKPSTEPLKSILKTGMPVMGMGRGLMPVDLNAELKNRLKRSTHATVSNLRKSATSADATRAASDEVDNPQRNLAQILRNVSKENSTPKHDDAVSLVRNLSTLGQPRSLARDDAAGNCASASEGESSGGREIEAIIKNSAVARRRRQQQQQSQQQNQPDG

Target ORF information:

RefSeq Version	NM_001202102.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster supervillin, transcript variant G (Svil), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202102.2

ORF Insert Sequence:

ATGCTGGCCC TCAAGGAGTG CCTCATACGG GAGGGTGTTC TCAATCTGGA CAGCCCCGAG 
AAGTACCGAC CGTACCATCT AAACGGCAGC AAAAGCAGCG GCGCCATGGA TTTTCCCCGA 
TATCGCAAAA CCATTGCGGC CTCAAGTGAC TTATCCACAG CACCTGCCAC TGCCAAGACC 
AGCTGCCAGC CACCTGAGCC ACTGAAACAG AAGCCACCGC AGCAGCAGAC CACCAAGGAC 
TCGGAGGATT CCAGTTCACA GAAGAGTGCT CCAGTCCAGT CGTATCGTGG TCAGCATCCG 
CACTTCCACC ATCACACCAC GCCCGCTTAC TACACGGATC TTCAGCTGAG AATTCACACG 
CCGAACGCAT CGGATGTGGG CGCCTTGAAC GAGAACTATC GAAGTGTATA CAGTACTCCG 
AGCACCCAGC TGGTGGAACA GCGATCGTTT CCAAAGAGCT CCACGGCCAC GAAGGATCCA 
CCGCTGCCCA TAAGCAATCC GGCTAGGGGA ACATTTTTTC GTGGCGACTC TGCGTCCAGC 
GACAGTCACT CAAGACTCCG TCGGAGCCAG CAGAGAACCG GCAGCGAGGG ACGCTTCTTC 
AGATACTCTC AGGGATCTGT GAGCCCTACC AAAGGTGAAC CTCCAATCGG CCACGAAGGT 
GGTGAGCAGC CACCCGTTGT CGCCAGTCAC TCCCCCATCA CATCCACCAG CACCACCACC 
ATCAGTAGTT GTAACCCCGT AGAGCAGGCT AGTCCACCAC CAACAAACGT AGTAAATCCC 
AGCGGATCCG GTCGCAACAG CCGGCACAAG CAGCGTTTCC ACAGTCGATC CCGTCGAGCG 
GTGCGCGTGC GCAGTGAATC GCGTCCGATT AGCGCCCTCT ATGACATAAT ATGCAAGGAG 
AAGAACCTGG AAAACAGCAG CAGCAGCAGC AGCAGCAGCA ACGAGCAGGA GAAGAAAAAG 
GATCAGGAGC AGCCTAAGAC CACAACCGAC AAGCTGGCGA CCTTTTGGCC CCTGCACCAT 
CACTACCTCA ATCCCCCGAT GAGCAGCGGC AACTCGGGCG GCAGCAGTGG CAATCCCAAG 
CAGCAGCAGC AGCAGCAGCA GCAACAACAG CTAGAGCAGC TGGGACACCA GACACATCTT 
GTGCTAGGAG CATCAAAGGA GTTGCCCGCG GTCAGCAGCA GCAGCGATGA AGAGGGATCC 
CTGCGGACTC TCCACTCCAG TCGCAAGGTG ACCAATCTGA ATGCCAAGTC CACCTCTCTG 
CCACCCGAGG AAGGACTCTC GGGTGATGTC TCCCTGGAGT CGTCCTCCAA CCGACTGAAT 
CTGCGATTGG CCAGGAGCCT GGAGCCCTCA GTCCGCAATA TCACTGCATC CGCCTCCAAG 
GAAGTACAGA GCTATGATGC TGGGGAGCCT GTGAATACCA ATCGATTGTT TGTGGAACCG 
CCCAAGATAC CACTGTCTGC CATCCGAAAC TCTCTTGACG ATCACGACCT GGATATTGAG 
GAGTGCGACA GCACCACCCA GTTCGATCAG GGGGATATCT ACGATACCCT GGAACGCTGG 
AGCCATGGTG TCAGCTTGGG GGCACATCTA TTGAACTGCG CGAATGGCCA CGATCAATCT 
GATCCCGAGG ACGACCATCA AACCGATCAT CATCAGCATG CACATCAATC ATTGCCAGTA 
GAACCCAAGT CTCATCATCA TCAGCACCAC CAGGCCACCA ATGCGCGCAG ATTCATAACG 
CGCACCACGC GTTCCGCCAG TCGCCAGAGT TCCGCCGGAC ACTCCCCACC TCCGCCGCTG 
CATCGCAGTG ACGACTCCTC CAGCAGTTCA CCCTCACCGA CACGCAGACG GAATAAGCCA 
GGTGGTGCTC CTGCCGCCAC AGATCCACCA TCATCGTCGG CGAACGGGAA TAGTTCCGCT 
TATGAGACGG CAGCCACCAC TGTGATGACC CATGACCAGC AGCGAGATCC TCCAGTTGAA 
GTTCCTAAGG CTCTAAACGT CGAGCCACCG CAGCCAGAAG ACATCGTCCT GGGCTCCTGC 
CCCACTGCAT CTGCCCACTC AGCAGAGAGC CAAAGTCGAG GAAAAGCTGG CAAATGCAGT 
ATTAATTGCA CCTACAGCCA CAGCCACAGC ACCAACAACA ACTGCAACGC GCGACAACGC 
AACAGCAGCA ACATCAGCAG CAACAACACC ATCAGCAGCA ACAGCAGCAG CAGCAAAAGC 
ACTTGCAACA ACAATAGCAA ACAGCAACAC GCCACGCCCA TGCCGCCCCT AGCTAATGCC 
AACAAAGCCC CGTTAATTGT GCTTAAGACA GCCGCATCTG CAGCAGCATT GCCGTCCATT 
GCCGCAGCAG CAACATCCAG CGGCACGGCA GCAACATCTG GTGGTGTTGG TTCGCCCATC 
AAGCGTCGCA AGAATCCGCT GTCGCCGCTG CTCCTCGGCA GCCAGTGCCA GTCCACCATT 
GCGCCTCCGC AGGCCATTGC CAGTCCGCTC CTGAGCGGCG GCAGCTGCTC CTCCTCGACC 
ACCTCGCCCC AATCCTCGCC AGCGGTTCGC ACCACCAAGG CGAGTCGTCT GCGCGCCGCC 
GCGCTTGATA AAAAGAAGGA AGAGGAGCAA CGCAATCGCT ACAGTTCGCC CGTTTCACCA 
AAAGCCTCCA GCAACGGCGG AGTCCAAGCA CAACGTCGTC TTAGCAACTC GGAGCTATCG 
CCCAAGTCGC CAGGTGCCAG TGGATTCACT TCGGAGCCAG TCGCGTCAGC CACAGCGAAC 
AGAGTCCCGA CGAGATCTGC CTCCCATCCT GTGGACACAC CGCCCACACA AAGGCGACAG 
CCTCTGGTGG CCGTGAGTTC GGGCATCTCC ATGAAGCAGA GTCTCAGCGA GCTGCACTTC 
ACGGGTGGAG CAGTCAGTCC GCCCAAGGCT ACGGCGGAAT GCACTCTAAG GATGCGACCA 
CGTAGCTCCA CGATGAGCAC CGAGAACACG GAGAATGGCC GACGTGACCA CTTGGCTAAT 
CTGCAACTAA GTCCTGTGCA AAAGCAAAAG CGAACGCCCG AAGTATCACC ACGCCGTTTC 
GGAGATCGCG ACAAGTCGGC CAAACGGAAA TCGAATTTGA ATCGTTCGTT GACCGAGGAA 
GCCACCAAGG ATGGCGATGA GGTTGCCTCT TCGACCCGCC GGCGCCGACG TCGTGAACTG 
GGAATGGCTC GTCCCCTGGT TCCGGGCGAT GATGACGCCC TAAGAGATTT GCTAACGCCG 
CAGAAGAGTT CCTGCAGCGA GTCAACCAAC TCCATATCCG AACCCCATCT CCAGTTCATA 
CCAGCCGTTC GTCATCCGGT GGTTCCAGTA CGCACCATGA CTGCTGTGCT GCCTGCACCA 
CGACCGCTGC ATACTCCTGC GGCCCGCAAC GTCAGCCAGA AGTTCAATGA AAGGACCAAA 
ACAGATCTGG CCGAGATCAA GAAGATTGCT GAGCAATCGG AGGCGCAGGT GCAACAGCCT 
TCGGTGGATG ATAACCAGAA AATGTCAGCC ATAATGCGCA GGAAGAGCAC GGATGAGGGT 
GGATCAGCTT TGGGGGCAAC AAACCAGAAT TCACCACCTG CCCCAAACCA AATTGTATCC 
ATACTGAAGA AGAAGGAACA TGGACTTGGC GAGTGCAACT CCAGTGCTTC AAGCAATCCC 
TCGCCGGTGA CCTTCTCTTC TAGCGTGGTG GATCATCCTT TGGCTGGAGC TGGCCGGTGC 
AAACGGCAGG GAATACTCAA GAAGAGATCG AGTCTAGATG AATCGCGCTA CTACTCCCGA 
TCGCATTCAC CCGACGAACG TAGTATTCTC ATTAAGTCGG CCAGGAGGAA TTCCCTGGAG 
GAAGCTGGCA CTGGATTGAG TCCGGCTCAG GCCCATGGAA TCCTCAAGCA GAGCAGCTAC 
GACAGCAGCA AGAGCGACGG CTGCCCTTCG GCCACGGAGA GCCAGCCACA CAGTATCCTG 
AAAAAGAAGG ATTCGCTATC GACACCTTCG GACGGAGGAT GCCACAAGCA TGTCTCTATA 
TCGCAAGCTG TAACTTTAGC TGCTGCTGAA CTGGCTGCCC ATGATGGAAT CACCTTCGAT 
GATGGTGAGG AGCATGAGAT ACGACCGATC CTAAAGCAGG AGAGCACCTC CAGCGAGGAG 
GCTGTGCGTC CGCCAAAGCC CATACTCAAA AAGAAATCCT TTGGCGAGGC GGATGAACAC 
GAAATACGTC CTATATTGAA GAGCTCTCGT AAAAGCAGTC GAGAGGAGTT CGATTTGAGT 
GGCTTGGAAA ACGAGGTCAA CGATTCCCTA TCGTCAATCC TAAAGACAGA CTCGCCGTCC 
AAGCGTCGGT CGCTCGGTTC AGCTTTGCAT GATTTGGAGG AGTCCACCAC TTCCCCGAGC 
ATGCTCAAGA GGCGCACCCG TTCGCTGGAA CGTCAAGATG TCCAGCCTGG CATGGATTTG 
GCAGCTGCAC TGGATGCCAT CGCCACATCA CAGGCTACTC CCAGCACCCC GGTGGACTTC 
GTGACCACAC CAACAAGTAT ATCGGTGGCG GAGCGGATCA AGCGCATGGA GATGCTGTCG 
ACGCCCACTT CCCGTGCGGC GGGTGGGGCC AACAGCAGCT GGGAATCGCC ACTGCAGGCA 
TCGTCAGCAG AGCATGGAGC ACCCACCACC CCAAGGGTTC TCAAGCCAAG TGTGCTGCGC 
AGAGATTTGC AGAGGGAGCG GTACAAAACG CAACCCGTGA CCAACGAGGA GATGAGCTTC 
TTCAAAGCCA ACTCCGCGCC CTTCGACATC TCGGCTACAT CGGCCTTCAA GCCCACCAAG 
CCGCTGGACA TCCGCTTGAC CCACGCACCG CATGCACCGC TGATTTCGCC GATCACCGGA 
CAGCCACTCA GCCACGTGCC CGCGCCACTG CTGCTATCCT CGTCGACGAA CTCGGGAGCG 
GGATCCTCGT TCCGTCTGGC CCGCAGCTCC ACCCAACCGC TGCAGTCGCC GCGAGTTGTC 
CACCTGGCCG GTCAGGCTAC TGGTGGCTCC GCTTTGGCAC GCCAGAACTC GGTGAACGAC 
AGCATCAATC TCAGCGAGCA GCTAACCCTA TCCATCGGCA CGGACAGCGA GCACATCTTT 
GAGATGTCCG CTTTGGAGGA GGACTCGGCT ATTCAGTCGC TGAGCGACGA GACAGCCGCT 
TCCGCATCTG CCAACTCAAC ATCCACGACC ACTGCAACCA CCATGACACC GCCCAGTGGC 
CTGACGCGGA GCAACAGCGT TCGGGCCAGG GCCAATATGT TCCAGCAATT GCAGGAGCAG 
TCGCGCAACC AACGGGCAAC GGGAACCACC AACTCCCGTC AATCTCCACC AGCGTCGTCG 
GCGGAGGTGT CTTCAAGTGC AGCCGACAAT TCCATGCACA GTGACGAGCG GTCCACGCCA 
AATGCAACGA TAGATGCGGA ATTCGGAAAA CCCAGCACCG AACCACTTAA GAGCATTCTT 
AAGACGGGAA TGCCAGTGAT GGGTATGGGA CGCGGTTTAA TGCCTGTGGA TCTCAATGCC 
GAGCTGAAGA ACCGGCTCAA ACGCTCCACA CATGCCACTG TTTCCAATCT CCGCAAATCT 
GCCACCTCGG CAGATGCCAC TAGAGCAGCT AGTGATGAGG TGGACAATCC GCAGAGGAAT 
TTAGCCCAGA TCCTGAGGAA TGTTTCCAAG GAGAACTCCA CGCCGAAGCA CGATGATGCC 
GTGAGCCTAG TGCGGAATCT CTCTACTTTG GGACAACCTC GATCGCTGGC CCGTGACGAT 
GCAGCCGGGA ATTGTGCCTC GGCATCCGAA GGCGAGAGTT CGGGGGGCCG CGAGATCGAG 
GCCATAATCA AGAACAGCGC TGTGGCCAGA CGTCGCCGGC AGCAACAGCA GCAGTCGCAG 
CAGCAGAATC AGCCAGATGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Svil ( NM_001202103.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001189032.1 Drosophila melanogaster supervillin, transcript variant H (Svil), mRNA.

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CloneID	OFa20001
Clone ID Related Accession (Same CDS sequence)	NM_001202103.2
Accession Version	NM_001202103.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6087bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	supervillin, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001202103.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGGCCC TCAAGGAGTG CCTCATACGG GAGGGTGTTC TCAATCTGGA CAGCCCCGAG 
AAGTACCGAC CGTACCATCT AAACGGCAGC AAAAGCAGCG GCGCCATGGA TTTTCCCCGA 
TATCGCAAAA CCATTGCGGC CTCAAGTGAC TTATCCACAG CACCTGCCAC TGCCAAGACC 
AGCTGCCAGC CACCTGAGCC ACTGAAACAG AAGCCACCGC AGCAGCAGAC CACCAAGGAC 
TCGGAGGATT CCAGTTCACA GAAGAGTGCT CCAGTCCAGT CGTATCGTGG TCAGCATCCG 
CACTTCCACC ATCACACCAC GCCCGCTTAC TACACGGATC TTCAGCTGAG AATTCACACG 
CCGAACGCAT CGGATGTGGG CGCCTTGAAC GAGAACTATC GAAGTGTATA CAGTACTCCG 
AGCACCCAGC TGGTGGAACA GCGATCGTTT CCAAAGAGCT CCACGGCCAC GAAGGATCCA 
CCGCTGCCCA TAAGCAATCC GGCTAGGGGA ACATTTTTTC GTGGCGACTC TGCGTCCAGC 
GACAGTCACT CAAGACTCCG TCGGAGCCAG CAGAGAACCG GCAGCGAGGG ACGCTTCTTC 
AGATACTCTC AGGGATCTGT GAGCCCTACC AAAGGTGAAC CTCCAATCGG CCACGAAGGT 
GGTGAGCAGC CACCCGTTGT CGCCAGTCAC TCCCCCATCA CATCCACCAG CACCACCACC 
ATCAGTAGTT GTAACCCCGT AGAGCAGGCT AGTCCACCAC CAACAAACGT AGTAAATCCC 
AGCGGATCCG GTCGCAACAG CCGGCACAAG CAGCGTTTCC ACAGTCGATC CCGTCGAGCG 
GTGCGCGTGC GCAGTGAATC GCGTCCGATT AGCGCCCTCT ATGACATAAT ATGCAAGGAG 
AAGAACCTGG AAAACAGCAG CAGCAGCAGC AGCAGCAGCA ACGAGCAGGA GAAGAAAAAG 
GATCAGGAGC AGCCTAAGAC CACAACCGAC AAGCTGGCGA CCTTTTGGCC CCTGCACCAT 
CACTACCTCA ATCCCCCGAT GAGCAGCGGC AACTCGGGCG GCAGCAGTGG CAATCCCAAG 
CAGCAGCAGC AGCAGCAGCA GCAACAACAG CTAGAGCAGC TGGGACACCA GACACATCTT 
GTGCTAGGAG CATCAAAGGA GTTGCCCGCG GTCAGCAGCA GCAGCGATGA AGAGGGATCC 
CTGCGGACTC TCCACTCCAG TCGCAAGGTG ACCAATCTGA ATGCCAAGTC CACCTCTCTG 
CCACCCGAGG AAGGACTCTC GGGTGATGTC TCCCTGGAGT CGTCCTCCAA CCGACTGAAT 
CTGCGATTGG CCAGGAGCCT GGAGCCCTCA GTCCGCAATA TCACTGCATC CGCCTCCAAG 
GAAGTACAGA GCTATGATGC TGGGGAGCCT GTGAATACCA ATCGATTGTT TGTGGAACCG 
CCCAAGATAC CACTGTCTGC CATCCGAAAC TCTCTTGACG ATCACGACCT GGATATTGAG 
GAGTGCGACA GCACCACCCA GTTCGATCAG GGGGATATCT ACGATACCCT GGAACGCTGG 
AGCCATGGTG TCAGCTTGGG GGCACATCTA TTGAACTGCG CGAATGGCCA CGATCAATCT 
GATCCCGAGG ACGACCATCA AACCGATCAT CATCAGCATG CACATCAATC ATTGCCAGTA 
GAACCCAAGT CTCATCATCA TCAGCACCAC CAGGCCACCA ATGCGCGCAG ATTCATAACG 
CGCACCACGC GTTCCGCCAG TCGCCAGAGT TCCGCCGGAC ACTCCCCACC TCCGCCGCTG 
CATCGCAGTG ACGACTCCTC CAGCAGTTCA CCCTCACCGA CACGCAGACG GAATAAGCCA 
GGTGGTGCTC CTGCCGCCAC AGATCCACCA TCATCGTCGG CGAACGGGAA TAGTTCCGCT 
TATGAGACGG CAGCCACCAC TGTGATGACC CATGACCAGC AGCGAGATCC TCCAGTTGAA 
GTTCCTAAGG CTCTAAACGT CGAGCCACCG CAGCCAGAAG ACATCGTCCT GGGCTCCTGC 
CCCACTGCAT CTGCCCACTC AGAGAGCCAA AGTCGAGGAA AAGCTGGCAA ATGCAGTATT 
AATTGCACCT ACAGCCACAG CCACAGCACC AACAACAACT GCAACGCGCG ACAACGCAAC 
AGCAGCAACA TCAGCAGCAA CAACACCATC AGCAGCAACA GCAGCAGCAG CAAAAGCACT 
TGCAACAACA ATAGCAAACA GCAACACGCC ACGCCCATGC CGCCCCTAGC TAATGCCAAC 
AAAGCCCCGT TAATTGTGCT TAAGACAGCC GCATCTGCAG CAGCATTGCC GTCCATTGCC 
GCAGCAGCAA CATCCAGCGG CACGGCAGCA ACATCTGGTG GTGTTGGTTC GCCCATCAAG 
CGTCGCAAGA ATCCGCTGTC GCCGCTGCTC CTCGGCAGCC AGTGCCAGTC CACCATTGCG 
CCTCCGCAGG CCATTGCCAG TCCGCTCCTG AGCGGCGGCA GCTGCTCCTC CTCGACCACC 
TCGCCCCAAT CCTCGCCAGC GGTTCGCACC ACCAAGGCGA GTCGTCTGCG CGCCGCCGCG 
CTTGATAAAA AGAAGGAAGA GGAGCAACGC AATCGCTACA GTTCGCCCGT TTCACCAAAA 
GCCTCCAGCA ACGGCGGAGT CCAAGCACAA CGTCGTCTTA GCAACTCGGA GCTATCGCCC 
AAGTCGCCAG GTGCCAGTGG ATTCACTTCG GAGCCAGTCG CGTCAGCCAC AGCGAACAGA 
GTCCCGACGA GATCTGCCTC CCATCCTGTG GACACACCGC CCACACAAAG GCGACAGCCT 
CTGGTGGCCG TGAGTTCGGG CATCTCCATG AAGCAGAGTC TCAGCGAGCT GCACTTCACG 
GGTGGAGCAG TCAGTCCGCC CAAGGCTACG GCGGAATGCA CTCTAAGGAT GCGACCACGT 
AGCTCCACGA TGAGCACCGA GAACACGGAG AATGGCCGAC GTGACCACTT GGCTAATCTG 
CAACTAAGTC CTGTGCAAAA GCAAAAGCGA ACGCCCGAAG TATCACCACG CCGTTTCGGA 
GATCGCGACA AGTCGGCCAA ACGGAAATCG AATTTGAATC GTTCGTTGAC CGAGGAAGCC 
ACCAAGGATG GCGATGAGGT TGCCTCTTCG ACCCGCCGGC GCCGACGTCG TGAACTGGGA 
ATGGCTCGTC CCCTGGTTCC GGGCGATGAT GACGCCCTAA GAGATTTGCT AACGCCGCAG 
AAGAGTTCCT GCAGCGAGTC AACCAACTCC ATATCCGAAC CCCATCTCCA GTTCATACCA 
GCCGTTCGTC ATCCGGTGGT TCCAGTACGC ACCATGACTG CTGTGCTGCC TGCACCACGA 
CCGCTGCATA CTCCTGCGGC CCGCAACGTC AGCCAGAAGT TCAATGAAAG GACCAAAACA 
GATCTGGCCG AGATCAAGAA GATTGCTGAG CAATCGGAGG CGCAGGTGCA ACAGCCTTCG 
GTGGATGATA ACCAGAAAAT GTCAGCCATA ATGCGCAGGA AGAGCACGGA TGAGGGTGGA 
TCAGCTTTGG GGGCAACAAA CCAGAATTCA CCACCTGCCC CAAACCAAAT TGTATCCATA 
CTGAAGAAGA AGGAACATGG ACTTGGCGAG TGCAACTCCA GTGCTTCAAG CAATCCCTCG 
CCGGTGACCT TCTCTTCTAG CGTGGTGGAT CATCCTTTGG CTGGAGCTGG CCGGTGCAAA 
CGGCAGGGAA TACTCAAGAA GAGATCGAGT CTAGATGAAT CGCGCTACTA CTCCCGATCG 
CATTCACCCG ACGAACGTAG TATTCTCATT AAGTCGGCCA GGAGGAATTC CCTGGAGGAA 
GCTGGCACTG GATTGAGTCC GGCTCAGGCC CATGGAATCC TCAAGCAGAG CAGCTACGAC 
AGCAGCAAGA GCGACGGCTG CCCTTCGGCC ACGGAGAGCC AGCCACACAG TATCCTGAAA 
AAGAAGGATT CGCTATCGAC ACCTTCGGAC GGAGGATGCC ACAAGCATGT CTCTATATCG 
CAAGCTGTAA CTTTAGCTGC TGCTGAACTG GCTGCCCATG ATGGAATCAC CTTCGATGAT 
GGTGAGGAGC ATGAGATACG ACCGATCCTA AAGCAGGAGA GCACCTCCAG CGAGGAGGCT 
GTGCGTCCGC CAAAGCCCAT ACTCAAAAAG AAATCCTTTG GCGAGGCGGA TGAACACGAA 
ATACGTCCTA TATTGAAGAG CTCTCGTAAA AGCAGTCGAG AGGAGTTCGA TTTGAGTGGC 
TTGGAAAACG AGGTCAACGA TTCCCTATCG TCAATCCTAA AGACAGACTC GCCGTCCAAG 
CGTCGGTCGC TCGGTTCAGC TTTGCATGAT TTGGAGGAGT CCACCACTTC CCCGAGCATG 
CTCAAGAGGC GCACCCGTTC GCTGGAACGT CAAGATGTCC AGCCTGGCAT GGATTTGGCA 
GCTGCACTGG ATGCCATCGC CACATCACAG GCTACTCCCA GCACCCCGGT GGACTTCGTG 
ACCACACCAA CAAGTATATC GGTGGCGGAG CGGATCAAGC GCATGGAGAT GCTGTCGACG 
CCCACTTCCC GTGCGGCGGG TGGGGCCAAC AGCAGCTGGG AATCGCCACT GCAGGCATCG 
TCAGCAGAGC ATGGAGCACC CACCACCCCA AGGGTTCTCA AGCCAAGTGT GCTGCGCAGA 
GATTTGCAGA GGGAGCGGTA CAAAACGCAA CCCGTGACCA ACGAGGAGAT GAGCTTCTTC 
AAAGCCAACT CCGCGCCCTT CGACATCTCG GCTACATCGG CCTTCAAGCC CACCAAGCCG 
CTGGACATCC GCTTGACCCA CGCACCGCAT GCACCGCTGA TTTCGCCGAT CACCGGACAG 
CCACTCAGCC ACGTGCCCGC GCCACTGCTG CTATCCTCGT CGACGAACTC GGGAGCGGGA 
TCCTCGTTCC GTCTGGCCCG CAGCTCCACC CAACCGCTGC AGTCGCCGCG AGTTGTCCAC 
CTGGCCGGTC AGGCTACTGG TGGCTCCGCT TTGGCACGCC AGAACTCGGT GAACGACAGC 
ATCAATCTCA GCGAGCAGCT AACCCTATCC ATCGGCACGG ACAGCGAGCA CATCTTTGAG 
ATGTCCGCTT TGGAGGAGGA CTCGGCTATT CAGTCGCTGA GCGACGAGAC AGCCGCTTCC 
GCATCTGCCA ACTCAACATC CACGACCACT GCAACCACCA TGACACCGCC CAGTGGCCTG 
ACGCGGAGCA ACAGCGTTCG GGCCAGGGCC AATATGTTCC AGCAATTGCA GGAGCAGTCG 
CGCAACCAAC GGGCAACGGG AACCACCAAC TCCCGTCAAT CTCCACCAGC GTCGTCGGCG 
GAGGTGTCTT CAAGTGCAGC CGACAATTCC ATGCACAGTG ACGAGCGGTC CACGCCAAAT 
GCAACGATAG ATGCGGAATT CGGAAAACCC AGCACCGAAC CACTTAAGAG CATTCTTAAG 
ACGGGAATGC CAGTGATGGG TATGGGACGC GGTTTAATGC CTGTGGATCT CAATGCCGAG 
CTGAAGAACC GGCTCAAACG CTCCACACAT GCCACTGTTT CCAATCTCCG CAAATCTGCC 
ACCTCGGCAG ATGCCACTAG AGCAGCTAGT GATGAGGTGG ACAATCCGCA GAGGAATTTA 
GCCCAGATCC TGAGGAATGT TTCCAAGGAG AACTCCACGC CGAAGCACGA TGATGCCGTG 
AGCCTAGTGC GGAATCTCTC TACTTTGGGA CAACCTCGAT CGCTGGCCCG TGACGATGCA 
GCCGGGAATT GTGCCTCGGC ATCCGAAGGC GAGAGTTCGG GGGGCCGCGA GATCGAGGCC 
ATAATCAAGA ACAGCGCTGT GGCCAGACGT CGCCGGCAGC AACAGCAGCA GTCGCAGCAG 
CAGAATCAGC CAGATGGATT CCTAGTCAGA GTGCAGCCCG TGGCAAGGAG CTACTACTAC 
TGGCCACTTG ACCAGCGGCC ACAGCAACAT CAGCCGCATC CGCCCCAGCA ACAAGGCCAA 
CAGCAGCAGC AGCCAGTGTT GCTCATGCAG CAGCAACCGA AACCCCTATA CAAGCTTCTT 
CAGCCCAGAC ATTTTGTAAA AAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189032.1
CDS	394..6480
Translation	MLALKECLIREGVLNLDSPEKYRPYHLNGSKSSGAMDFPRYRKTIAASSDLSTAPATAKTSCQPPEPLKQKPPQQQTTKDSEDSSSQKSAPVQSYRGQHPHFHHHTTPAYYTDLQLRIHTPNASDVGALNENYRSVYSTPSTQLVEQRSFPKSSTATKDPPLPISNPARGTFFRGDSASSDSHSRLRRSQQRTGSEGRFFRYSQGSVSPTKGEPPIGHEGGEQPPVVASHSPITSTSTTTISSCNPVEQASPPPTNVVNPSGSGRNSRHKQRFHSRSRRAVRVRSESRPISALYDIICKEKNLENSSSSSSSSNEQEKKKDQEQPKTTTDKLATFWPLHHHYLNPPMSSGNSGGSSGNPKQQQQQQQQQQLEQLGHQTHLVLGASKELPAVSSSSDEEGSLRTLHSSRKVTNLNAKSTSLPPEEGLSGDVSLESSSNRLNLRLARSLEPSVRNITASASKEVQSYDAGEPVNTNRLFVEPPKIPLSAIRNSLDDHDLDIEECDSTTQFDQGDIYDTLERWSHGVSLGAHLLNCANGHDQSDPEDDHQTDHHQHAHQSLPVEPKSHHHQHHQATNARRFITRTTRSASRQSSAGHSPPPPLHRSDDSSSSSPSPTRRRNKPGGAPAATDPPSSSANGNSSAYETAATTVMTHDQQRDPPVEVPKALNVEPPQPEDIVLGSCPTASAHSESQSRGKAGKCSINCTYSHSHSTNNNCNARQRNSSNISSNNTISSNSSSSKSTCNNNSKQQHATPMPPLANANKAPLIVLKTAASAAALPSIAAAATSSGTAATSGGVGSPIKRRKNPLSPLLLGSQCQSTIAPPQAIASPLLSGGSCSSSTTSPQSSPAVRTTKASRLRAAALDKKKEEEQRNRYSSPVSPKASSNGGVQAQRRLSNSELSPKSPGASGFTSEPVASATANRVPTRSASHPVDTPPTQRRQPLVAVSSGISMKQSLSELHFTGGAVSPPKATAECTLRMRPRSSTMSTENTENGRRDHLANLQLSPVQKQKRTPEVSPRRFGDRDKSAKRKSNLNRSLTEEATKDGDEVASSTRRRRRRELGMARPLVPGDDDALRDLLTPQKSSCSESTNSISEPHLQFIPAVRHPVVPVRTMTAVLPAPRPLHTPAARNVSQKFNERTKTDLAEIKKIAEQSEAQVQQPSVDDNQKMSAIMRRKSTDEGGSALGATNQNSPPAPNQIVSILKKKEHGLGECNSSASSNPSPVTFSSSVVDHPLAGAGRCKRQGILKKRSSLDESRYYSRSHSPDERSILIKSARRNSLEEAGTGLSPAQAHGILKQSSYDSSKSDGCPSATESQPHSILKKKDSLSTPSDGGCHKHVSISQAVTLAAAELAAHDGITFDDGEEHEIRPILKQESTSSEEAVRPPKPILKKKSFGEADEHEIRPILKSSRKSSREEFDLSGLENEVNDSLSSILKTDSPSKRRSLGSALHDLEESTTSPSMLKRRTRSLERQDVQPGMDLAAALDAIATSQATPSTPVDFVTTPTSISVAERIKRMEMLSTPTSRAAGGANSSWESPLQASSAEHGAPTTPRVLKPSVLRRDLQRERYKTQPVTNEEMSFFKANSAPFDISATSAFKPTKPLDIRLTHAPHAPLISPITGQPLSHVPAPLLLSSSTNSGAGSSFRLARSSTQPLQSPRVVHLAGQATGGSALARQNSVNDSINLSEQLTLSIGTDSEHIFEMSALEEDSAIQSLSDETAASASANSTSTTTATTMTPPSGLTRSNSVRARANMFQQLQEQSRNQRATGTTNSRQSPPASSAEVSSSAADNSMHSDERSTPNATIDAEFGKPSTEPLKSILKTGMPVMGMGRGLMPVDLNAELKNRLKRSTHATVSNLRKSATSADATRAASDEVDNPQRNLAQILRNVSKENSTPKHDDAVSLVRNLSTLGQPRSLARDDAAGNCASASEGESSGGREIEAIIKNSAVARRRRQQQQQSQQQNQPDGFLVRVQPVARSYYYWPLDQRPQQHQPHPPQQQGQQQQQPVLLMQQQPKPLYKLLQPRHFVKN

Target ORF information:

RefSeq Version	NM_001202103.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster supervillin, transcript variant H (Svil), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202103.2

ORF Insert Sequence:

ATGCTGGCCC TCAAGGAGTG CCTCATACGG GAGGGTGTTC TCAATCTGGA CAGCCCCGAG 
AAGTACCGAC CGTACCATCT AAACGGCAGC AAAAGCAGCG GCGCCATGGA TTTTCCCCGA 
TATCGCAAAA CCATTGCGGC CTCAAGTGAC TTATCCACAG CACCTGCCAC TGCCAAGACC 
AGCTGCCAGC CACCTGAGCC ACTGAAACAG AAGCCACCGC AGCAGCAGAC CACCAAGGAC 
TCGGAGGATT CCAGTTCACA GAAGAGTGCT CCAGTCCAGT CGTATCGTGG TCAGCATCCG 
CACTTCCACC ATCACACCAC GCCCGCTTAC TACACGGATC TTCAGCTGAG AATTCACACG 
CCGAACGCAT CGGATGTGGG CGCCTTGAAC GAGAACTATC GAAGTGTATA CAGTACTCCG 
AGCACCCAGC TGGTGGAACA GCGATCGTTT CCAAAGAGCT CCACGGCCAC GAAGGATCCA 
CCGCTGCCCA TAAGCAATCC GGCTAGGGGA ACATTTTTTC GTGGCGACTC TGCGTCCAGC 
GACAGTCACT CAAGACTCCG TCGGAGCCAG CAGAGAACCG GCAGCGAGGG ACGCTTCTTC 
AGATACTCTC AGGGATCTGT GAGCCCTACC AAAGGTGAAC CTCCAATCGG CCACGAAGGT 
GGTGAGCAGC CACCCGTTGT CGCCAGTCAC TCCCCCATCA CATCCACCAG CACCACCACC 
ATCAGTAGTT GTAACCCCGT AGAGCAGGCT AGTCCACCAC CAACAAACGT AGTAAATCCC 
AGCGGATCCG GTCGCAACAG CCGGCACAAG CAGCGTTTCC ACAGTCGATC CCGTCGAGCG 
GTGCGCGTGC GCAGTGAATC GCGTCCGATT AGCGCCCTCT ATGACATAAT ATGCAAGGAG 
AAGAACCTGG AAAACAGCAG CAGCAGCAGC AGCAGCAGCA ACGAGCAGGA GAAGAAAAAG 
GATCAGGAGC AGCCTAAGAC CACAACCGAC AAGCTGGCGA CCTTTTGGCC CCTGCACCAT 
CACTACCTCA ATCCCCCGAT GAGCAGCGGC AACTCGGGCG GCAGCAGTGG CAATCCCAAG 
CAGCAGCAGC AGCAGCAGCA GCAACAACAG CTAGAGCAGC TGGGACACCA GACACATCTT 
GTGCTAGGAG CATCAAAGGA GTTGCCCGCG GTCAGCAGCA GCAGCGATGA AGAGGGATCC 
CTGCGGACTC TCCACTCCAG TCGCAAGGTG ACCAATCTGA ATGCCAAGTC CACCTCTCTG 
CCACCCGAGG AAGGACTCTC GGGTGATGTC TCCCTGGAGT CGTCCTCCAA CCGACTGAAT 
CTGCGATTGG CCAGGAGCCT GGAGCCCTCA GTCCGCAATA TCACTGCATC CGCCTCCAAG 
GAAGTACAGA GCTATGATGC TGGGGAGCCT GTGAATACCA ATCGATTGTT TGTGGAACCG 
CCCAAGATAC CACTGTCTGC CATCCGAAAC TCTCTTGACG ATCACGACCT GGATATTGAG 
GAGTGCGACA GCACCACCCA GTTCGATCAG GGGGATATCT ACGATACCCT GGAACGCTGG 
AGCCATGGTG TCAGCTTGGG GGCACATCTA TTGAACTGCG CGAATGGCCA CGATCAATCT 
GATCCCGAGG ACGACCATCA AACCGATCAT CATCAGCATG CACATCAATC ATTGCCAGTA 
GAACCCAAGT CTCATCATCA TCAGCACCAC CAGGCCACCA ATGCGCGCAG ATTCATAACG 
CGCACCACGC GTTCCGCCAG TCGCCAGAGT TCCGCCGGAC ACTCCCCACC TCCGCCGCTG 
CATCGCAGTG ACGACTCCTC CAGCAGTTCA CCCTCACCGA CACGCAGACG GAATAAGCCA 
GGTGGTGCTC CTGCCGCCAC AGATCCACCA TCATCGTCGG CGAACGGGAA TAGTTCCGCT 
TATGAGACGG CAGCCACCAC TGTGATGACC CATGACCAGC AGCGAGATCC TCCAGTTGAA 
GTTCCTAAGG CTCTAAACGT CGAGCCACCG CAGCCAGAAG ACATCGTCCT GGGCTCCTGC 
CCCACTGCAT CTGCCCACTC AGAGAGCCAA AGTCGAGGAA AAGCTGGCAA ATGCAGTATT 
AATTGCACCT ACAGCCACAG CCACAGCACC AACAACAACT GCAACGCGCG ACAACGCAAC 
AGCAGCAACA TCAGCAGCAA CAACACCATC AGCAGCAACA GCAGCAGCAG CAAAAGCACT 
TGCAACAACA ATAGCAAACA GCAACACGCC ACGCCCATGC CGCCCCTAGC TAATGCCAAC 
AAAGCCCCGT TAATTGTGCT TAAGACAGCC GCATCTGCAG CAGCATTGCC GTCCATTGCC 
GCAGCAGCAA CATCCAGCGG CACGGCAGCA ACATCTGGTG GTGTTGGTTC GCCCATCAAG 
CGTCGCAAGA ATCCGCTGTC GCCGCTGCTC CTCGGCAGCC AGTGCCAGTC CACCATTGCG 
CCTCCGCAGG CCATTGCCAG TCCGCTCCTG AGCGGCGGCA GCTGCTCCTC CTCGACCACC 
TCGCCCCAAT CCTCGCCAGC GGTTCGCACC ACCAAGGCGA GTCGTCTGCG CGCCGCCGCG 
CTTGATAAAA AGAAGGAAGA GGAGCAACGC AATCGCTACA GTTCGCCCGT TTCACCAAAA 
GCCTCCAGCA ACGGCGGAGT CCAAGCACAA CGTCGTCTTA GCAACTCGGA GCTATCGCCC 
AAGTCGCCAG GTGCCAGTGG ATTCACTTCG GAGCCAGTCG CGTCAGCCAC AGCGAACAGA 
GTCCCGACGA GATCTGCCTC CCATCCTGTG GACACACCGC CCACACAAAG GCGACAGCCT 
CTGGTGGCCG TGAGTTCGGG CATCTCCATG AAGCAGAGTC TCAGCGAGCT GCACTTCACG 
GGTGGAGCAG TCAGTCCGCC CAAGGCTACG GCGGAATGCA CTCTAAGGAT GCGACCACGT 
AGCTCCACGA TGAGCACCGA GAACACGGAG AATGGCCGAC GTGACCACTT GGCTAATCTG 
CAACTAAGTC CTGTGCAAAA GCAAAAGCGA ACGCCCGAAG TATCACCACG CCGTTTCGGA 
GATCGCGACA AGTCGGCCAA ACGGAAATCG AATTTGAATC GTTCGTTGAC CGAGGAAGCC 
ACCAAGGATG GCGATGAGGT TGCCTCTTCG ACCCGCCGGC GCCGACGTCG TGAACTGGGA 
ATGGCTCGTC CCCTGGTTCC GGGCGATGAT GACGCCCTAA GAGATTTGCT AACGCCGCAG 
AAGAGTTCCT GCAGCGAGTC AACCAACTCC ATATCCGAAC CCCATCTCCA GTTCATACCA 
GCCGTTCGTC ATCCGGTGGT TCCAGTACGC ACCATGACTG CTGTGCTGCC TGCACCACGA 
CCGCTGCATA CTCCTGCGGC CCGCAACGTC AGCCAGAAGT TCAATGAAAG GACCAAAACA 
GATCTGGCCG AGATCAAGAA GATTGCTGAG CAATCGGAGG CGCAGGTGCA ACAGCCTTCG 
GTGGATGATA ACCAGAAAAT GTCAGCCATA ATGCGCAGGA AGAGCACGGA TGAGGGTGGA 
TCAGCTTTGG GGGCAACAAA CCAGAATTCA CCACCTGCCC CAAACCAAAT TGTATCCATA 
CTGAAGAAGA AGGAACATGG ACTTGGCGAG TGCAACTCCA GTGCTTCAAG CAATCCCTCG 
CCGGTGACCT TCTCTTCTAG CGTGGTGGAT CATCCTTTGG CTGGAGCTGG CCGGTGCAAA 
CGGCAGGGAA TACTCAAGAA GAGATCGAGT CTAGATGAAT CGCGCTACTA CTCCCGATCG 
CATTCACCCG ACGAACGTAG TATTCTCATT AAGTCGGCCA GGAGGAATTC CCTGGAGGAA 
GCTGGCACTG GATTGAGTCC GGCTCAGGCC CATGGAATCC TCAAGCAGAG CAGCTACGAC 
AGCAGCAAGA GCGACGGCTG CCCTTCGGCC ACGGAGAGCC AGCCACACAG TATCCTGAAA 
AAGAAGGATT CGCTATCGAC ACCTTCGGAC GGAGGATGCC ACAAGCATGT CTCTATATCG 
CAAGCTGTAA CTTTAGCTGC TGCTGAACTG GCTGCCCATG ATGGAATCAC CTTCGATGAT 
GGTGAGGAGC ATGAGATACG ACCGATCCTA AAGCAGGAGA GCACCTCCAG CGAGGAGGCT 
GTGCGTCCGC CAAAGCCCAT ACTCAAAAAG AAATCCTTTG GCGAGGCGGA TGAACACGAA 
ATACGTCCTA TATTGAAGAG CTCTCGTAAA AGCAGTCGAG AGGAGTTCGA TTTGAGTGGC 
TTGGAAAACG AGGTCAACGA TTCCCTATCG TCAATCCTAA AGACAGACTC GCCGTCCAAG 
CGTCGGTCGC TCGGTTCAGC TTTGCATGAT TTGGAGGAGT CCACCACTTC CCCGAGCATG 
CTCAAGAGGC GCACCCGTTC GCTGGAACGT CAAGATGTCC AGCCTGGCAT GGATTTGGCA 
GCTGCACTGG ATGCCATCGC CACATCACAG GCTACTCCCA GCACCCCGGT GGACTTCGTG 
ACCACACCAA CAAGTATATC GGTGGCGGAG CGGATCAAGC GCATGGAGAT GCTGTCGACG 
CCCACTTCCC GTGCGGCGGG TGGGGCCAAC AGCAGCTGGG AATCGCCACT GCAGGCATCG 
TCAGCAGAGC ATGGAGCACC CACCACCCCA AGGGTTCTCA AGCCAAGTGT GCTGCGCAGA 
GATTTGCAGA GGGAGCGGTA CAAAACGCAA CCCGTGACCA ACGAGGAGAT GAGCTTCTTC 
AAAGCCAACT CCGCGCCCTT CGACATCTCG GCTACATCGG CCTTCAAGCC CACCAAGCCG 
CTGGACATCC GCTTGACCCA CGCACCGCAT GCACCGCTGA TTTCGCCGAT CACCGGACAG 
CCACTCAGCC ACGTGCCCGC GCCACTGCTG CTATCCTCGT CGACGAACTC GGGAGCGGGA 
TCCTCGTTCC GTCTGGCCCG CAGCTCCACC CAACCGCTGC AGTCGCCGCG AGTTGTCCAC 
CTGGCCGGTC AGGCTACTGG TGGCTCCGCT TTGGCACGCC AGAACTCGGT GAACGACAGC 
ATCAATCTCA GCGAGCAGCT AACCCTATCC ATCGGCACGG ACAGCGAGCA CATCTTTGAG 
ATGTCCGCTT TGGAGGAGGA CTCGGCTATT CAGTCGCTGA GCGACGAGAC AGCCGCTTCC 
GCATCTGCCA ACTCAACATC CACGACCACT GCAACCACCA TGACACCGCC CAGTGGCCTG 
ACGCGGAGCA ACAGCGTTCG GGCCAGGGCC AATATGTTCC AGCAATTGCA GGAGCAGTCG 
CGCAACCAAC GGGCAACGGG AACCACCAAC TCCCGTCAAT CTCCACCAGC GTCGTCGGCG 
GAGGTGTCTT CAAGTGCAGC CGACAATTCC ATGCACAGTG ACGAGCGGTC CACGCCAAAT 
GCAACGATAG ATGCGGAATT CGGAAAACCC AGCACCGAAC CACTTAAGAG CATTCTTAAG 
ACGGGAATGC CAGTGATGGG TATGGGACGC GGTTTAATGC CTGTGGATCT CAATGCCGAG 
CTGAAGAACC GGCTCAAACG CTCCACACAT GCCACTGTTT CCAATCTCCG CAAATCTGCC 
ACCTCGGCAG ATGCCACTAG AGCAGCTAGT GATGAGGTGG ACAATCCGCA GAGGAATTTA 
GCCCAGATCC TGAGGAATGT TTCCAAGGAG AACTCCACGC CGAAGCACGA TGATGCCGTG 
AGCCTAGTGC GGAATCTCTC TACTTTGGGA CAACCTCGAT CGCTGGCCCG TGACGATGCA 
GCCGGGAATT GTGCCTCGGC ATCCGAAGGC GAGAGTTCGG GGGGCCGCGA GATCGAGGCC 
ATAATCAAGA ACAGCGCTGT GGCCAGACGT CGCCGGCAGC AACAGCAGCA GTCGCAGCAG 
CAGAATCAGC CAGATGGATT CCTAGTCAGA GTGCAGCCCG TGGCAAGGAG CTACTACTAC 
TGGCCACTTG ACCAGCGGCC ACAGCAACAT CAGCCGCATC CGCCCCAGCA ACAAGGCCAA 
CAGCAGCAGC AGCCAGTGTT GCTCATGCAG CAGCAACCGA AACCCCTATA CAAGCTTCTT 
CAGCCCAGAC ATTTTGTAAA AAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Svil ( NM_001299988.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286917.1 Drosophila melanogaster supervillin, transcript variant AC (Svil), mRNA.

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CloneID	OFa21973
Clone ID Related Accession (Same CDS sequence)	NM_001299988.1
Accession Version	NM_001299988.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	supervillin, isoform AC
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCGGC TAAGGGGCAA TCACAGCATT GGAGGATCCT CGCAGCAGCT TTCTGGCAAA 
CAGAAATGGT CCTTCGGGCG ACTTTTTCGC AAGAAAAAGG AGGCGGAGAG TGCATCCTCC 
AGCGAAGAAG ATCGCAAGGC GGGATTTGTG CCTGCCCAGC GCCAGTCAGC CAGCAAGCCG 
AAGGGAAAAC ATGGCAAAGG ATCGCGAGGT TGCAGCAAAT TCGATCACAT TGTGGTGAGT 
CAGCGGCAAG AGCAGGCTCC ACCACATCCA CCCCAACCGC AAATCGACAA TGAGTTCTTC 
TTGCCCTTGG AGGCCATACA ACCGGAGCCC TACTATCAAA ATCAACCGGG CTACTATCAA 
CGATCGAGTA ACTCGCTGGA CAGGAGATTA CTAATGCAGC CTGGAGTTCA GGGAAATGGT 
TACCAGCAGC CCAAATCTCG ATCGGCGCAG CGAGGTCCCA AGCCACCAGG AGCTCACTCC 
AGCGAGGAGG AACTGATCTC GCTGAACTCC TCCACGTTCT CCAAATACCG CAGCGATGAG 
AGTATTCATT TGGGTTCCGC TGCTGGACAA AGTCGACGCA GTCGAGCGGC CCGCAATGAG 
CGTTACTACA AGAGATTGTC CCGGGATGGC GAACCGAGTC ATGGCGGAGC ACCCGTTGTA 
GCCCAGCAGC GATACAAAAC CCAGCCACTG CCTTTATCCA TTTACCAGCC AACGAACGCG 
TCCACTGGCT ATGTCCAGTG GCAACCGCAG CCCCAGAAGA ACCTGCAGAA TGCGATGCAG 
AACGATGGAA AGCGAAGCAT CAGCTACGAC AGTCAAATAC ACCTGCAGAA CATGAATGGG 
AGGATGCAGA GTAAGCCATT GCCACCGCCA CCGCCTCCAA GGGATCCACT GAGGAGAGTG 
CATGTTGGAG GTTCCGGGAA GATTGGATCC AGCTCTGGAG ACTTGCGTCC CGTGTCCTAT 
GCCTTCGATC ACAATAGTCG CTGTGCGTCG GATGATCGCA TTTGGCAGCC ACCCCACTAC 
CAGTCGGTGC ATTCGCTCAA CTCCCAGCCC AGTTCGTCAA TAGTCAGTGC ACCAGTGAGT 
CAACAGCAAC CGCACCACAG GAGATTCATA ACTCGATCCG AGAGGAACGC AAATCCTGGC 
AAGCAATCCC TGCCGAATGG TATTGATTTC CATTATGTGG CAGATGCAAC GCCACGCTCC 
AGGAAGCCCA TACACATGAT GGATGCAGGC AGGATGGACA ATACAGGAGT GCCAGCATCC 
AGTGGTATAT TGCGAACCTC TAAGAGTGGG CTGCCCACAC CAAGTCCTGC TCCCCAGCAG 
CCCTTGGCCA AACCGCGGTC GTTCTCCAGC TCGCGACTTA GTGAGCTGCG AACCTATCCC 
ATGCCCATGT ACTCCGAGGT GCAGAAACCT AAGAGGAATG CGCCACCAGT CAATCCCTCG 
CCGAATCAGG ATAACATAGT GTGTGGGAGT CTGCACATAA AACCCACACA AGAGCATGGA 
AATCAAAACT ATGTGGAGAT TCGTCATCGG GATGTGCACA AGACCAACTC AATGCCGCAC 
CATTATCAGA ATAGGCGATC TGGCGAGGAT TTACCCAACA AATATGAGGA GTACGTGGCA 
GAAAAGAGGG AGCAGCATCA ACAGGCACCA CCCCCAGTTT ATCCGGAGCG CAAAATGAGC 
TTGACACCAG CAGTGGCGCC ACCGCCTCCT GTTTACTTCC CTCGCAAGAA GCCTGCCAAT 
TTGGAGGAGG CCATCAACGA ACTGGAGGCC ATTTACAAGT CTCTAGGACT CACTGAACCA 
GCCGAGCCCA AACAGGAACC AAAGCTGGAT ATTTCCTCCA AGATGCGTGT GCCAACTCCC 
AGTGATTTTG AAAAGTTCGC CTTGGCGCAT GCTGATGACT ACGAGGATGA AGATTCGCCA 
ACCGGAGAAC CAGATCCAAT CAGAGATGAT GTGGCCTTCA GGAACCTACA GTTGGCCAAT 
CTGCAACATC GGCCATCGGA GAAGCAGCCT CCATTCGGCA TCCCAGTGGG ACCCATTGTG 
CCAGCTCCAC AGTCGGATTA TCTTCACGTA GAGCCAGTGA GGGTGAAGAA GAAGAGTGGC 
TCTCCTGACA TCATTAAAGA TGATCTTGCT GTGCGTTCAT TGAGAAAGGA TCCTCCTGGT 
CCCAAATCCA GCTTTGTCTA CCCAATGCAA AAGAAGCAAC GGGCTATTCG AACCCAATCG 
GCCAACATAT ACAATCTGAT CCAAAGAGAC GCTGCCAAGC CATCTGGTGG AGATCTTAAG 
AGCTACATGG AGCTGACTCG CAACCTGGAA AGAGCTGGCA GCATGTCCAA TCTGCAGGGT 
GAGGATGGCA GTGATACCAA AGATGTACCC GCCACACTGG ATCTTCTCCG AAAGTTGAAA 
GCTCAGGATG AGGAACTGGA TTTGGCTAGA AAGCATCACC CCATACCGTT TAGACATCCC 
AGTCAGGGAG GAGCTATTGC CCAACTGCCA GAACGGTTGT CCAAAGAGGA AATACCCAAG 
GCAGCGCCTG TGCCAGCTCC CCGAAAGAGT CTGACTCCTG AACCCATGCT AGATGATGCT 
CTCAACAAGA TTGCCCAGGA TGCGCAGGCC AGCAGCATTA AGCTGAGTCA GGAACTCCAT 
GAACTTCGCA AGGAGGCATT GATTACAGCT GCGAGACCAA AGTTGAATGC CGAACAACAG 
AGATTAGAGG ATGAACTGCA GCAGATAGAA GCTGTCTCCG AGGCAGCCAA ACGTTGTGGC 
CAAATGCTGC TCGAAACTCT GCCAGATGCG GGACAGGAGG GTCAGGAACC TCAGGATAAG 
CCCCTCAGGA AGCTACATAA GGAGGGCAAA CTCATACGAG CTATCGATGA GGTGTCCGAA 
GCTGCTAATG CGGTCTGTGA GAAGATCCTC AAGGACATTG TGACCACAGA ACCACCGGTG 
GTAGTTCAGG AGGCCCTCCA AGTGAGGCAG ACCACAAAGA CTGGACCAGG CGGAGAACAG 
CTGGTGATGC CGCATCTTAT CAAGAAACTA GATCCCATAC AATCCGACAA GATCGAGCAC 
ATTGCCAGAC GATGCATGCG GCAGTTGAGC GAACTGGCCA ATAATCCGGA CTACGACAAC 
CTGGCCACCT GTAATATGAG CAACAGCACC ACCAACGCAA CGGGTGGAGC AGCCGCTGAT 
GCCACTCTCG TTTGTCCCGT TCCAGAGACC CAAGCCAAGA GCCAGACGCT CGAGGAGATT 
GACAAGATCA TGCAGGAGTG CGAGCGTCAG GCGAAGAGCA GCAGCTCAAC CACGACCACC 
GATGATCAGC GCACCACTGC CCCCAGTCTG ACGAGTGGAA GTGGCAGCGG GAGCTTCCCA 
CCCAATGTGA GCACCACCGC CAGTTCGTTC AGCTCAAGCA GCGACTGTTT GGCCAAGTCG 
TCGAGTCCTT CGCGCGCCAG CCGTCCGCTC TCCTCGAGCA TCACCTCTTT CAATCCATAT 
TCCTCCAGCG ACTACATCAA GTCGCAGAGC AGTGATTGCC ATGCACCAAG CACCGATCCG 
ATCAAGACCT TTAGCACCAC CTCGTACGAG GTGCAGTCCA CACCGCAGAG CACCACCATT 
AGCACCAATA CGATGAGCAC GAGCCAGAGC AACACGATCA GTAGCACTAC TAATCCGAGT 
GTGGAGTCCA AGCCATCCGC CCCGTCGGTA TCGCCACCAC CCCTCCAGTT GACGCCAGTG 
GGTGATCGGA GGGGCAGGAA CCGGGAGCGG GGAGCTTCCT GCTCCTCCAC ATCCTCACCT 
TCACAGTACA ACTCGAGCGA GGAGCTGGCC GCCATTTTTG GCATCGAGGA GCAACCGAAA 
CAGCAGCGTC GCAGTCGGCA GACGCATAAT GCCACAGAAA CCATTGCGGC CTCAAGTGAC 
TTATCCACAG CACCTGCCAC TGCCAAGACC AGCTGCCAGC CACCTGAGCC ACTGAAACAG 
AAGCCACCGC AGCAGCAGAC CACCAAGGAC TCGGAGGATT CCAGTTCACA GAAGAGTGCT 
CCAGTCCAGT CGTATCGTGG TCAGCATCCG CACTTCCACC ATCACACCAC GCCCGCTTAC 
TACACGGATC TTCAGCTGAG AATTCACACG CCGAACGCAT CGGATGTGGG CGCCTTGAAC 
GAGAACTATC GAAGTGTATA CAGTACTCCG AGCACCCAGC TGGTGGAACA GCGATCGTTT 
CCAAAGAGCT CCACGGCCAC GAAGGATCCA CCGCTGCCCA TAAGCAATCC GGCTAGGGGA 
ACATTTTTTC GTGGCGACTC TGCGTCCAGC GACAGTCACT CAAGACTCCG TCGGAGCCAG 
CAGAGAACCG GCAGCGAGGG ACGCTTCTTC AGATACTCTC AGGGATCTGT GAGCCCTACC 
AAAGGTGAAC CTCCAATCGG CCACGAAGGT GGTGAGCAGC CACCCGTTGT CGCCAGTCAC 
TCCCCCATCA CATCCACCAG CACCACCACC ATCAGTAGTT GTAACCCCGT AGAGCAGGCT 
AGTCCACCAC CAACAAACGT AGTAAATCCC AGCGGATCCG GTCGCAACAG CCGGCACAAG 
CAGCGTTTCC ACAGTCGATC CCGTCGAGCG GTGCGCGTGC GCAGTGAATC GCGTCCGATT 
AGCGCCCTCT ATGACATAAT ATGCAAGGAG AAGAACCTGG AAAACAGCAG CAGCAGCAGC 
AGCAGCAGCA ACGAGCAGGA GAAGAAAAAG GATCAGGAGC AGCCTAAGAC CACAACCGAC 
AAGCTGGCGA CCTTTTGGCC CCTGCACCAT CACTACCTCA ATCCCCCGAT GAGCAGCGGC 
AACTCGGGCG GCAGCAGTGG CAATCCCAAG CAGCAGCAGC AGCAGCAGCA GCAACAACAG 
CTAGAGCAGC TGGGACACCA GACACATCTT GTGCTAGGAG CATCAAAGGA GTTGCCCGCG 
GTCAGCAGCA GCAGCGATGA AGAGGGATCC CTGCGGACTC TCCACTCCAG TCGCAAGGTG 
ACCAATCTGA ATGCCAAGTC CACCTCTCTG CCACCCGAGG AAGGACTCTC GGGTGATGTC 
TCCCTGGAGT CGTCCTCCAA CCGACTGAAT CTGCGATTGG CCAGGAGCCT GGAGCCCTCA 
GTCCGCAATA TCACTGCATC CGCCTCCAAG GAAGTACAGA GCTATGATGC TGGGGAGCCT 
GTGAATACCA ATCGATTGTT TGTGGAACCG CCCAAGATAC CACTGTCTGC CATCCGAAAC 
TCTCTTGACG ATCACGACCT GGATATTGAG GAGTGCGACA GCACCACCCA GTTCGATCAG 
GGGGATATCT ACGATACCCT GGAACGCTGG AGCCATGGTG TCAGCTTGGG GGCACATCTA 
TTGAACTGCG CGAATGGCCA CGATCAATCT GATCCCGAGG ACGACCATCA AACCGATCAT 
CATCAGCATG CACATCAATC ATTGCCAGTA GAACCCAAGT CTCATCATCA TCAGCACCAC 
CAGGCCACCA ATGCGCGCAG ATTCATAACG CGCACCACGC GTTCCGCCAG TCGCCAGAGT 
TCCGCCGGAC ACTCCCCACC TCCGCCGCTG CATCGCAGTG ACGACTCCTC CAGCAGTTCA 
CCCTCACCGA CACGCAGACG GAATAAGCCA GGTGGTGCTC CTGCCGCCAC AGATCCACCA 
TCATCGTCGG CGAACGGGAA TAGTTCCGCT TATGAGACGG CAGCCACCAC TGTGATGACC 
CATGACCAGC AGCGAGATCC TCCAGTTGAA GTTCCTAAGG CTCTAAACGT CGAGCCACCG 
CAGCCAGAAG ACATCGTCCT GGGCTCCTGC CCCACTGCAT CTGCCCACTC AGATGAGGTT 
GCCTCTTCGA CCCGCCGGCG CCGACGTCGT GAACTGGGAA TGGCTCGTCC CCTGGTTCCG 
GGCGATGATG ACGCCCTAAG AGATTTGCTA ACGCCGCAGA AGAGTTCCTG CAGCGAGTCA 
ACCAACTCCA TATCCGAACC CCATCTCCAG TTCATACCAG CCGTTCGTCA TCCGGTGGTT 
CCAGTACGCA CCATGACTGC TGTGCTGCCT GCACCACGAC CGCTGCATAC TCCTGCGGCC 
CGCAACGTCA GCCAGAAGTT CAATGAAAGG ACCAAAACAG ATCTGGCCGA GATCAAGAAG 
ATTGCTGAGC AATCGGAGGC GCAGGTGCAA CAGCCTTCGG TGGATGATAA CCAGAAAATG 
TCAGCCATAA TGCGCAGGAA GAGCACGGAT GAGGGTGGAT CAGCTTTGGG GGCAACAAAC 
CAGAATTCAC CACCTGCCCC AAACCAAATT GTATCCATAC TGAAGAAGAA GGAACATGGA 
CTTGGCGAGT GCAACTCCAG TGCTTCAAGC AATCCCTCGC CGGTGACCTT CTCTTCTAGC 
GTGGTGGATC ATCCTTTGGC TGGAGCTGGC CGGTGCAAAC GGCAGGGAAT ACTCAAGAAG 
AGATCGAGTC TAGATGAATC GCGCTACTAC TCCCGATCGC ATTCACCCGA CGAACGTAGT 
ATTCTCATTA AGTCGGCCAG GAGGAATTCC CTGGAGGAAG CTGGCACTGG ATTGAGTCCG 
GCTCAGGCCC ATGGAATCCT CAAGCAGAGC AGCTACGACA GCAGCAAGAG CGACGGCTGC 
CCTTCGGCCA CGGAGAGCCA GCCACACAGT ATCCTGAAAA AGAAGGATTC GCTATCGACA 
CCTTCGGACG GAGGATGCCA CAAGCATGTC TCTATATCGC AAGCTGTAAC TTTAGCTGCT 
GCTGAACTGG CTGCCCATGA TGGAATCACC TTCGATGATG GTGAGGAGCA TGAGATACGA 
CCGATCCTAA AGCAGGAGAG CACCTCCAGC GAGGAGGCTG TGCGTCCGCC AAAGCCCATA 
CTCAAAAAGA AATCCTTTGG CGAGGCGGAT GAACACGAAA TACGTCCTAT ATTGAAGAGC 
TCTCGTAAAA GCAGTCGAGA GGAGTTCGAT TTGAGTGGCT TGGAAAACGA GGTCAACGAT 
TCCCTATCGT CAATCCTAAA GACAGACTCG CCGTCCAAGC GTCGGTCGCT CGGTTCAGCT 
TTGCATGATT TGGAGGAGTC CACCACTTCC CCGAGCATGC TCAAGAGGCG CACCCGTTCG 
CTGGAACGTC AAGATGTCCA GCCTGGCATG GATTTGGCAG CTGCACTGGA TGCCATCGCC 
ACATCACAGG CTACTCCCAG CACCCCGGTG GACTTCGTGA CCACACCAAC AAGTATATCG 
GTGGCGGAGC GGATCAAGCG CATGGAGATG CTGTCGACGC CCACTTCCCG TGCGGCGGGT 
GGGGCCAACA GCAGCTGGGA ATCGCCACTG CAGGCATCGT CAGCAGAGCA TGGAGCACCC 
ACCACCCCAA GGGTTCTCAA GCCAAGTGTG CTGCGCAGAG ATTTGCAGAG GGAGCGGTAC 
AAAACGCAAC CCGTGACCAA CGAGGAGATG AGCTTCTTCA AAGCCAACTC CGCGCCCTTC 
GACATCTCGG CTACATCGGC CTTCAAGCCC ACCAAGCCGC TGGACATCCG CTTGACCCAC 
GCACCGCATG CACCGCTGAT TTCGCCGATC ACCGGACAGC CACTCAGCCA CGTGCCCGCG 
CCACTGCTGC TATCCTCGTC GACGAACTCG GGAGCGGGAT CCTCGTTCCG TCTGGCCCGC 
AGCTCCACCC AACCGCTGCA GTCGCCGCGA GTTGTCCACC TGGCCGGTCA GGCTACTGGT 
GGCTCCGCTT TGGCACGCCA GAACTCGGTG AACGACAGCA TCAATCTCAG CGAGCAGCTA 
ACCCTATCCA TCGGCACGGA CAGCGAGCAC ATCTTTGAGA TGTCCGCTTT GGAGGAGGAC 
TCGGCTATTC AGTCGCTGAG CGACGAGACA GCCGCTTCCG CATCTGCCAA CTCAACATCC 
ACGACCACTG CAACCACCAT GACACCGCCC AGTGGCCTGA CGCGGAGCAA CAGCGTTCGG 
GCCAGGGCCA ATATGTTCCA GCAATTGCAG GAGCAGTCGC GCAACCAACG GGCAACGGGA 
ACCACCAACT CCCGTCAATC TCCACCAGCG TCGTCGGCGG AGGTGTCTTC AAGTGCAGCC 
GACAATTCCA TGCACAGTGA CGAGCGGTCC ACGCCAAATG CAACGATAGA TGCGGAATTC 
GAACCCTCAT CCCTGTCGCT GGCTGAGCGA CTGGCCTTCT TCAGCAATCT GTGCGAGAGC 
GGCGGAGGAG GTGGGAGTAC CAGTGCCGGT GGAAGGTACC GCAGTCGCAC CAGCAGCTAT 
TCAAGGAGTC CGCCCGGCGA TCGCACCACT CCCAGGAGCA GCACCACCGC CAGCAGTGTC 
AGTATGACGC CCACGCCCAT GGATTACTCT CCGATTCCCC ACGACAAGGA GCCATCTAGT 
CTCACGCTGG AGAGCATCAT CGAGCCCGAG CAGGAGGAGG TCAAGGAGCA GGTGGACCAG 
GATGAGGTCA AGTTGAGGAA GAAGGAGCCA GTCCAGAGGG AATCTTCCCA GCACAATGGC 
TTCCACGTGC TGACCAGGTC CGCCACCGAA CCGCCGTCGT CCACCTCGCC CCTGCGCATC 
AGCATACGGA CTGTGGGAAA GCTAGTCCTG CCCGATACCT TCCGCGGCGA TCGCAACAAT 
AACAGCAGTA GTAGAAGTGG GAGCAACAGC TGGAGCAACA CTTGCATGGT CAAGCTGCAG 
AGCTTGGAGC CCGTTAGCCT GTCCGTTCAT TCGCGCACCA TTGGTAAGGT TAAGTCGCCA 
TTCATTGAAA AGCAGCAGGA GAAACCACAC ACCGAGAAGC TAGCGAACGG AACCAGCGAC 
AGTGGCTCCG ATTCGGGCAA GGAGAACTGC GCCTCCAACG AGCAGGAACA TCTGCAGCAC 
CAGCAGTCGC AGGACGAGGA TCGAGCTCCG CCCAGGGTGT CCGAGATGCG GAGGAAAATC 
AGCCGACTGG TGCAACAACA GCAGCCGCAA CCACAGCCCG TCAATCGGCG GCACACCACC 
GAGATCACCT CCGTGACCGT TGGAACGCGT TCGCCCAGTG TGGACGATCG CTTTGCCAAG 
TACTTTGGTG TTAAGGAGAG CTGCAACAGC ACGACCACCG TGCATAAAAC GCAATCGCTG 
CCACCCAGCC AAGTTGAGGC CACCAATGCG CACGTGAAAC TACCGCAGAT CACTACCGTG 
GTTTCCAAAC GGCGCGAGCT GCTCAAGAGG ACGCGACCCC TGTCCATGGA TCACTACTCC 
AGCGGCTGCA CCAGTCCCAC AGCCATGCCA AGTCCCAAGA GAGCACATTC GCCCGTCGGC 
CGGCGGAAGG CCGCCATTTC CAGCATCGAG GACATTGAGG TCACGCCGGA TGAACTCCGG 
GCCGCTGGCA GGAACTTCAA GCTGTTGTAC GGCGAGTTAT TGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286917.1
CDS	786..10133
Translation	MDRLRGNHSIGGSSQQLSGKQKWSFGRLFRKKKEAESASSSEEDRKAGFVPAQRQSASKPKGKHGKGSRGCSKFDHIVVSQRQEQAPPHPPQPQIDNEFFLPLEAIQPEPYYQNQPGYYQRSSNSLDRRLLMQPGVQGNGYQQPKSRSAQRGPKPPGAHSSEEELISLNSSTFSKYRSDESIHLGSAAGQSRRSRAARNERYYKRLSRDGEPSHGGAPVVAQQRYKTQPLPLSIYQPTNASTGYVQWQPQPQKNLQNAMQNDGKRSISYDSQIHLQNMNGRMQSKPLPPPPPPRDPLRRVHVGGSGKIGSSSGDLRPVSYAFDHNSRCASDDRIWQPPHYQSVHSLNSQPSSSIVSAPVSQQQPHHRRFITRSERNANPGKQSLPNGIDFHYVADATPRSRKPIHMMDAGRMDNTGVPASSGILRTSKSGLPTPSPAPQQPLAKPRSFSSSRLSELRTYPMPMYSEVQKPKRNAPPVNPSPNQDNIVCGSLHIKPTQEHGNQNYVEIRHRDVHKTNSMPHHYQNRRSGEDLPNKYEEYVAEKREQHQQAPPPVYPERKMSLTPAVAPPPPVYFPRKKPANLEEAINELEAIYKSLGLTEPAEPKQEPKLDISSKMRVPTPSDFEKFALAHADDYEDEDSPTGEPDPIRDDVAFRNLQLANLQHRPSEKQPPFGIPVGPIVPAPQSDYLHVEPVRVKKKSGSPDIIKDDLAVRSLRKDPPGPKSSFVYPMQKKQRAIRTQSANIYNLIQRDAAKPSGGDLKSYMELTRNLERAGSMSNLQGEDGSDTKDVPATLDLLRKLKAQDEELDLARKHHPIPFRHPSQGGAIAQLPERLSKEEIPKAAPVPAPRKSLTPEPMLDDALNKIAQDAQASSIKLSQELHELRKEALITAARPKLNAEQQRLEDELQQIEAVSEAAKRCGQMLLETLPDAGQEGQEPQDKPLRKLHKEGKLIRAIDEVSEAANAVCEKILKDIVTTEPPVVVQEALQVRQTTKTGPGGEQLVMPHLIKKLDPIQSDKIEHIARRCMRQLSELANNPDYDNLATCNMSNSTTNATGGAAADATLVCPVPETQAKSQTLEEIDKIMQECERQAKSSSSTTTTDDQRTTAPSLTSGSGSGSFPPNVSTTASSFSSSSDCLAKSSSPSRASRPLSSSITSFNPYSSSDYIKSQSSDCHAPSTDPIKTFSTTSYEVQSTPQSTTISTNTMSTSQSNTISSTTNPSVESKPSAPSVSPPPLQLTPVGDRRGRNRERGASCSSTSSPSQYNSSEELAAIFGIEEQPKQQRRSRQTHNATETIAASSDLSTAPATAKTSCQPPEPLKQKPPQQQTTKDSEDSSSQKSAPVQSYRGQHPHFHHHTTPAYYTDLQLRIHTPNASDVGALNENYRSVYSTPSTQLVEQRSFPKSSTATKDPPLPISNPARGTFFRGDSASSDSHSRLRRSQQRTGSEGRFFRYSQGSVSPTKGEPPIGHEGGEQPPVVASHSPITSTSTTTISSCNPVEQASPPPTNVVNPSGSGRNSRHKQRFHSRSRRAVRVRSESRPISALYDIICKEKNLENSSSSSSSSNEQEKKKDQEQPKTTTDKLATFWPLHHHYLNPPMSSGNSGGSSGNPKQQQQQQQQQQLEQLGHQTHLVLGASKELPAVSSSSDEEGSLRTLHSSRKVTNLNAKSTSLPPEEGLSGDVSLESSSNRLNLRLARSLEPSVRNITASASKEVQSYDAGEPVNTNRLFVEPPKIPLSAIRNSLDDHDLDIEECDSTTQFDQGDIYDTLERWSHGVSLGAHLLNCANGHDQSDPEDDHQTDHHQHAHQSLPVEPKSHHHQHHQATNARRFITRTTRSASRQSSAGHSPPPPLHRSDDSSSSSPSPTRRRNKPGGAPAATDPPSSSANGNSSAYETAATTVMTHDQQRDPPVEVPKALNVEPPQPEDIVLGSCPTASAHSDEVASSTRRRRRRELGMARPLVPGDDDALRDLLTPQKSSCSESTNSISEPHLQFIPAVRHPVVPVRTMTAVLPAPRPLHTPAARNVSQKFNERTKTDLAEIKKIAEQSEAQVQQPSVDDNQKMSAIMRRKSTDEGGSALGATNQNSPPAPNQIVSILKKKEHGLGECNSSASSNPSPVTFSSSVVDHPLAGAGRCKRQGILKKRSSLDESRYYSRSHSPDERSILIKSARRNSLEEAGTGLSPAQAHGILKQSSYDSSKSDGCPSATESQPHSILKKKDSLSTPSDGGCHKHVSISQAVTLAAAELAAHDGITFDDGEEHEIRPILKQESTSSEEAVRPPKPILKKKSFGEADEHEIRPILKSSRKSSREEFDLSGLENEVNDSLSSILKTDSPSKRRSLGSALHDLEESTTSPSMLKRRTRSLERQDVQPGMDLAAALDAIATSQATPSTPVDFVTTPTSISVAERIKRMEMLSTPTSRAAGGANSSWESPLQASSAEHGAPTTPRVLKPSVLRRDLQRERYKTQPVTNEEMSFFKANSAPFDISATSAFKPTKPLDIRLTHAPHAPLISPITGQPLSHVPAPLLLSSSTNSGAGSSFRLARSSTQPLQSPRVVHLAGQATGGSALARQNSVNDSINLSEQLTLSIGTDSEHIFEMSALEEDSAIQSLSDETAASASANSTSTTTATTMTPPSGLTRSNSVRARANMFQQLQEQSRNQRATGTTNSRQSPPASSAEVSSSAADNSMHSDERSTPNATIDAEFEPSSLSLAERLAFFSNLCESGGGGGSTSAGGRYRSRTSSYSRSPPGDRTTPRSSTTASSVSMTPTPMDYSPIPHDKEPSSLTLESIIEPEQEEVKEQVDQDEVKLRKKEPVQRESSQHNGFHVLTRSATEPPSSTSPLRISIRTVGKLVLPDTFRGDRNNNSSSRSGSNSWSNTCMVKLQSLEPVSLSVHSRTIGKVKSPFIEKQQEKPHTEKLANGTSDSGSDSGKENCASNEQEHLQHQQSQDEDRAPPRVSEMRRKISRLVQQQQPQPQPVNRRHTTEITSVTVGTRSPSVDDRFAKYFGVKESCNSTTTVHKTQSLPPSQVEATNAHVKLPQITTVVSKRRELLKRTRPLSMDHYSSGCTSPTAMPSPKRAHSPVGRRKAAISSIEDIEVTPDELRAAGRNFKLLYGELLG

Target ORF information:

RefSeq Version	NM_001299988.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster supervillin, transcript variant AC (Svil), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299988.1

ORF Insert Sequence:

ATGGATCGGC TAAGGGGCAA TCACAGCATT GGAGGATCCT CGCAGCAGCT TTCTGGCAAA 
CAGAAATGGT CCTTCGGGCG ACTTTTTCGC AAGAAAAAGG AGGCGGAGAG TGCATCCTCC 
AGCGAAGAAG ATCGCAAGGC GGGATTTGTG CCTGCCCAGC GCCAGTCAGC CAGCAAGCCG 
AAGGGAAAAC ATGGCAAAGG ATCGCGAGGT TGCAGCAAAT TCGATCACAT TGTGGTGAGT 
CAGCGGCAAG AGCAGGCTCC ACCACATCCA CCCCAACCGC AAATCGACAA TGAGTTCTTC 
TTGCCCTTGG AGGCCATACA ACCGGAGCCC TACTATCAAA ATCAACCGGG CTACTATCAA 
CGATCGAGTA ACTCGCTGGA CAGGAGATTA CTAATGCAGC CTGGAGTTCA GGGAAATGGT 
TACCAGCAGC CCAAATCTCG ATCGGCGCAG CGAGGTCCCA AGCCACCAGG AGCTCACTCC 
AGCGAGGAGG AACTGATCTC GCTGAACTCC TCCACGTTCT CCAAATACCG CAGCGATGAG 
AGTATTCATT TGGGTTCCGC TGCTGGACAA AGTCGACGCA GTCGAGCGGC CCGCAATGAG 
CGTTACTACA AGAGATTGTC CCGGGATGGC GAACCGAGTC ATGGCGGAGC ACCCGTTGTA 
GCCCAGCAGC GATACAAAAC CCAGCCACTG CCTTTATCCA TTTACCAGCC AACGAACGCG 
TCCACTGGCT ATGTCCAGTG GCAACCGCAG CCCCAGAAGA ACCTGCAGAA TGCGATGCAG 
AACGATGGAA AGCGAAGCAT CAGCTACGAC AGTCAAATAC ACCTGCAGAA CATGAATGGG 
AGGATGCAGA GTAAGCCATT GCCACCGCCA CCGCCTCCAA GGGATCCACT GAGGAGAGTG 
CATGTTGGAG GTTCCGGGAA GATTGGATCC AGCTCTGGAG ACTTGCGTCC CGTGTCCTAT 
GCCTTCGATC ACAATAGTCG CTGTGCGTCG GATGATCGCA TTTGGCAGCC ACCCCACTAC 
CAGTCGGTGC ATTCGCTCAA CTCCCAGCCC AGTTCGTCAA TAGTCAGTGC ACCAGTGAGT 
CAACAGCAAC CGCACCACAG GAGATTCATA ACTCGATCCG AGAGGAACGC AAATCCTGGC 
AAGCAATCCC TGCCGAATGG TATTGATTTC CATTATGTGG CAGATGCAAC GCCACGCTCC 
AGGAAGCCCA TACACATGAT GGATGCAGGC AGGATGGACA ATACAGGAGT GCCAGCATCC 
AGTGGTATAT TGCGAACCTC TAAGAGTGGG CTGCCCACAC CAAGTCCTGC TCCCCAGCAG 
CCCTTGGCCA AACCGCGGTC GTTCTCCAGC TCGCGACTTA GTGAGCTGCG AACCTATCCC 
ATGCCCATGT ACTCCGAGGT GCAGAAACCT AAGAGGAATG CGCCACCAGT CAATCCCTCG 
CCGAATCAGG ATAACATAGT GTGTGGGAGT CTGCACATAA AACCCACACA AGAGCATGGA 
AATCAAAACT ATGTGGAGAT TCGTCATCGG GATGTGCACA AGACCAACTC AATGCCGCAC 
CATTATCAGA ATAGGCGATC TGGCGAGGAT TTACCCAACA AATATGAGGA GTACGTGGCA 
GAAAAGAGGG AGCAGCATCA ACAGGCACCA CCCCCAGTTT ATCCGGAGCG CAAAATGAGC 
TTGACACCAG CAGTGGCGCC ACCGCCTCCT GTTTACTTCC CTCGCAAGAA GCCTGCCAAT 
TTGGAGGAGG CCATCAACGA ACTGGAGGCC ATTTACAAGT CTCTAGGACT CACTGAACCA 
GCCGAGCCCA AACAGGAACC AAAGCTGGAT ATTTCCTCCA AGATGCGTGT GCCAACTCCC 
AGTGATTTTG AAAAGTTCGC CTTGGCGCAT GCTGATGACT ACGAGGATGA AGATTCGCCA 
ACCGGAGAAC CAGATCCAAT CAGAGATGAT GTGGCCTTCA GGAACCTACA GTTGGCCAAT 
CTGCAACATC GGCCATCGGA GAAGCAGCCT CCATTCGGCA TCCCAGTGGG ACCCATTGTG 
CCAGCTCCAC AGTCGGATTA TCTTCACGTA GAGCCAGTGA GGGTGAAGAA GAAGAGTGGC 
TCTCCTGACA TCATTAAAGA TGATCTTGCT GTGCGTTCAT TGAGAAAGGA TCCTCCTGGT 
CCCAAATCCA GCTTTGTCTA CCCAATGCAA AAGAAGCAAC GGGCTATTCG AACCCAATCG 
GCCAACATAT ACAATCTGAT CCAAAGAGAC GCTGCCAAGC CATCTGGTGG AGATCTTAAG 
AGCTACATGG AGCTGACTCG CAACCTGGAA AGAGCTGGCA GCATGTCCAA TCTGCAGGGT 
GAGGATGGCA GTGATACCAA AGATGTACCC GCCACACTGG ATCTTCTCCG AAAGTTGAAA 
GCTCAGGATG AGGAACTGGA TTTGGCTAGA AAGCATCACC CCATACCGTT TAGACATCCC 
AGTCAGGGAG GAGCTATTGC CCAACTGCCA GAACGGTTGT CCAAAGAGGA AATACCCAAG 
GCAGCGCCTG TGCCAGCTCC CCGAAAGAGT CTGACTCCTG AACCCATGCT AGATGATGCT 
CTCAACAAGA TTGCCCAGGA TGCGCAGGCC AGCAGCATTA AGCTGAGTCA GGAACTCCAT 
GAACTTCGCA AGGAGGCATT GATTACAGCT GCGAGACCAA AGTTGAATGC CGAACAACAG 
AGATTAGAGG ATGAACTGCA GCAGATAGAA GCTGTCTCCG AGGCAGCCAA ACGTTGTGGC 
CAAATGCTGC TCGAAACTCT GCCAGATGCG GGACAGGAGG GTCAGGAACC TCAGGATAAG 
CCCCTCAGGA AGCTACATAA GGAGGGCAAA CTCATACGAG CTATCGATGA GGTGTCCGAA 
GCTGCTAATG CGGTCTGTGA GAAGATCCTC AAGGACATTG TGACCACAGA ACCACCGGTG 
GTAGTTCAGG AGGCCCTCCA AGTGAGGCAG ACCACAAAGA CTGGACCAGG CGGAGAACAG 
CTGGTGATGC CGCATCTTAT CAAGAAACTA GATCCCATAC AATCCGACAA GATCGAGCAC 
ATTGCCAGAC GATGCATGCG GCAGTTGAGC GAACTGGCCA ATAATCCGGA CTACGACAAC 
CTGGCCACCT GTAATATGAG CAACAGCACC ACCAACGCAA CGGGTGGAGC AGCCGCTGAT 
GCCACTCTCG TTTGTCCCGT TCCAGAGACC CAAGCCAAGA GCCAGACGCT CGAGGAGATT 
GACAAGATCA TGCAGGAGTG CGAGCGTCAG GCGAAGAGCA GCAGCTCAAC CACGACCACC 
GATGATCAGC GCACCACTGC CCCCAGTCTG ACGAGTGGAA GTGGCAGCGG GAGCTTCCCA 
CCCAATGTGA GCACCACCGC CAGTTCGTTC AGCTCAAGCA GCGACTGTTT GGCCAAGTCG 
TCGAGTCCTT CGCGCGCCAG CCGTCCGCTC TCCTCGAGCA TCACCTCTTT CAATCCATAT 
TCCTCCAGCG ACTACATCAA GTCGCAGAGC AGTGATTGCC ATGCACCAAG CACCGATCCG 
ATCAAGACCT TTAGCACCAC CTCGTACGAG GTGCAGTCCA CACCGCAGAG CACCACCATT 
AGCACCAATA CGATGAGCAC GAGCCAGAGC AACACGATCA GTAGCACTAC TAATCCGAGT 
GTGGAGTCCA AGCCATCCGC CCCGTCGGTA TCGCCACCAC CCCTCCAGTT GACGCCAGTG 
GGTGATCGGA GGGGCAGGAA CCGGGAGCGG GGAGCTTCCT GCTCCTCCAC ATCCTCACCT 
TCACAGTACA ACTCGAGCGA GGAGCTGGCC GCCATTTTTG GCATCGAGGA GCAACCGAAA 
CAGCAGCGTC GCAGTCGGCA GACGCATAAT GCCACAGAAA CCATTGCGGC CTCAAGTGAC 
TTATCCACAG CACCTGCCAC TGCCAAGACC AGCTGCCAGC CACCTGAGCC ACTGAAACAG 
AAGCCACCGC AGCAGCAGAC CACCAAGGAC TCGGAGGATT CCAGTTCACA GAAGAGTGCT 
CCAGTCCAGT CGTATCGTGG TCAGCATCCG CACTTCCACC ATCACACCAC GCCCGCTTAC 
TACACGGATC TTCAGCTGAG AATTCACACG CCGAACGCAT CGGATGTGGG CGCCTTGAAC 
GAGAACTATC GAAGTGTATA CAGTACTCCG AGCACCCAGC TGGTGGAACA GCGATCGTTT 
CCAAAGAGCT CCACGGCCAC GAAGGATCCA CCGCTGCCCA TAAGCAATCC GGCTAGGGGA 
ACATTTTTTC GTGGCGACTC TGCGTCCAGC GACAGTCACT CAAGACTCCG TCGGAGCCAG 
CAGAGAACCG GCAGCGAGGG ACGCTTCTTC AGATACTCTC AGGGATCTGT GAGCCCTACC 
AAAGGTGAAC CTCCAATCGG CCACGAAGGT GGTGAGCAGC CACCCGTTGT CGCCAGTCAC 
TCCCCCATCA CATCCACCAG CACCACCACC ATCAGTAGTT GTAACCCCGT AGAGCAGGCT 
AGTCCACCAC CAACAAACGT AGTAAATCCC AGCGGATCCG GTCGCAACAG CCGGCACAAG 
CAGCGTTTCC ACAGTCGATC CCGTCGAGCG GTGCGCGTGC GCAGTGAATC GCGTCCGATT 
AGCGCCCTCT ATGACATAAT ATGCAAGGAG AAGAACCTGG AAAACAGCAG CAGCAGCAGC 
AGCAGCAGCA ACGAGCAGGA GAAGAAAAAG GATCAGGAGC AGCCTAAGAC CACAACCGAC 
AAGCTGGCGA CCTTTTGGCC CCTGCACCAT CACTACCTCA ATCCCCCGAT GAGCAGCGGC 
AACTCGGGCG GCAGCAGTGG CAATCCCAAG CAGCAGCAGC AGCAGCAGCA GCAACAACAG 
CTAGAGCAGC TGGGACACCA GACACATCTT GTGCTAGGAG CATCAAAGGA GTTGCCCGCG 
GTCAGCAGCA GCAGCGATGA AGAGGGATCC CTGCGGACTC TCCACTCCAG TCGCAAGGTG 
ACCAATCTGA ATGCCAAGTC CACCTCTCTG CCACCCGAGG AAGGACTCTC GGGTGATGTC 
TCCCTGGAGT CGTCCTCCAA CCGACTGAAT CTGCGATTGG CCAGGAGCCT GGAGCCCTCA 
GTCCGCAATA TCACTGCATC CGCCTCCAAG GAAGTACAGA GCTATGATGC TGGGGAGCCT 
GTGAATACCA ATCGATTGTT TGTGGAACCG CCCAAGATAC CACTGTCTGC CATCCGAAAC 
TCTCTTGACG ATCACGACCT GGATATTGAG GAGTGCGACA GCACCACCCA GTTCGATCAG 
GGGGATATCT ACGATACCCT GGAACGCTGG AGCCATGGTG TCAGCTTGGG GGCACATCTA 
TTGAACTGCG CGAATGGCCA CGATCAATCT GATCCCGAGG ACGACCATCA AACCGATCAT 
CATCAGCATG CACATCAATC ATTGCCAGTA GAACCCAAGT CTCATCATCA TCAGCACCAC 
CAGGCCACCA ATGCGCGCAG ATTCATAACG CGCACCACGC GTTCCGCCAG TCGCCAGAGT 
TCCGCCGGAC ACTCCCCACC TCCGCCGCTG CATCGCAGTG ACGACTCCTC CAGCAGTTCA 
CCCTCACCGA CACGCAGACG GAATAAGCCA GGTGGTGCTC CTGCCGCCAC AGATCCACCA 
TCATCGTCGG CGAACGGGAA TAGTTCCGCT TATGAGACGG CAGCCACCAC TGTGATGACC 
CATGACCAGC AGCGAGATCC TCCAGTTGAA GTTCCTAAGG CTCTAAACGT CGAGCCACCG 
CAGCCAGAAG ACATCGTCCT GGGCTCCTGC CCCACTGCAT CTGCCCACTC AGATGAGGTT 
GCCTCTTCGA CCCGCCGGCG CCGACGTCGT GAACTGGGAA TGGCTCGTCC CCTGGTTCCG 
GGCGATGATG ACGCCCTAAG AGATTTGCTA ACGCCGCAGA AGAGTTCCTG CAGCGAGTCA 
ACCAACTCCA TATCCGAACC CCATCTCCAG TTCATACCAG CCGTTCGTCA TCCGGTGGTT 
CCAGTACGCA CCATGACTGC TGTGCTGCCT GCACCACGAC CGCTGCATAC TCCTGCGGCC 
CGCAACGTCA GCCAGAAGTT CAATGAAAGG ACCAAAACAG ATCTGGCCGA GATCAAGAAG 
ATTGCTGAGC AATCGGAGGC GCAGGTGCAA CAGCCTTCGG TGGATGATAA CCAGAAAATG 
TCAGCCATAA TGCGCAGGAA GAGCACGGAT GAGGGTGGAT CAGCTTTGGG GGCAACAAAC 
CAGAATTCAC CACCTGCCCC AAACCAAATT GTATCCATAC TGAAGAAGAA GGAACATGGA 
CTTGGCGAGT GCAACTCCAG TGCTTCAAGC AATCCCTCGC CGGTGACCTT CTCTTCTAGC 
GTGGTGGATC ATCCTTTGGC TGGAGCTGGC CGGTGCAAAC GGCAGGGAAT ACTCAAGAAG 
AGATCGAGTC TAGATGAATC GCGCTACTAC TCCCGATCGC ATTCACCCGA CGAACGTAGT 
ATTCTCATTA AGTCGGCCAG GAGGAATTCC CTGGAGGAAG CTGGCACTGG ATTGAGTCCG 
GCTCAGGCCC ATGGAATCCT CAAGCAGAGC AGCTACGACA GCAGCAAGAG CGACGGCTGC 
CCTTCGGCCA CGGAGAGCCA GCCACACAGT ATCCTGAAAA AGAAGGATTC GCTATCGACA 
CCTTCGGACG GAGGATGCCA CAAGCATGTC TCTATATCGC AAGCTGTAAC TTTAGCTGCT 
GCTGAACTGG CTGCCCATGA TGGAATCACC TTCGATGATG GTGAGGAGCA TGAGATACGA 
CCGATCCTAA AGCAGGAGAG CACCTCCAGC GAGGAGGCTG TGCGTCCGCC AAAGCCCATA 
CTCAAAAAGA AATCCTTTGG CGAGGCGGAT GAACACGAAA TACGTCCTAT ATTGAAGAGC 
TCTCGTAAAA GCAGTCGAGA GGAGTTCGAT TTGAGTGGCT TGGAAAACGA GGTCAACGAT 
TCCCTATCGT CAATCCTAAA GACAGACTCG CCGTCCAAGC GTCGGTCGCT CGGTTCAGCT 
TTGCATGATT TGGAGGAGTC CACCACTTCC CCGAGCATGC TCAAGAGGCG CACCCGTTCG 
CTGGAACGTC AAGATGTCCA GCCTGGCATG GATTTGGCAG CTGCACTGGA TGCCATCGCC 
ACATCACAGG CTACTCCCAG CACCCCGGTG GACTTCGTGA CCACACCAAC AAGTATATCG 
GTGGCGGAGC GGATCAAGCG CATGGAGATG CTGTCGACGC CCACTTCCCG TGCGGCGGGT 
GGGGCCAACA GCAGCTGGGA ATCGCCACTG CAGGCATCGT CAGCAGAGCA TGGAGCACCC 
ACCACCCCAA GGGTTCTCAA GCCAAGTGTG CTGCGCAGAG ATTTGCAGAG GGAGCGGTAC 
AAAACGCAAC CCGTGACCAA CGAGGAGATG AGCTTCTTCA AAGCCAACTC CGCGCCCTTC 
GACATCTCGG CTACATCGGC CTTCAAGCCC ACCAAGCCGC TGGACATCCG CTTGACCCAC 
GCACCGCATG CACCGCTGAT TTCGCCGATC ACCGGACAGC CACTCAGCCA CGTGCCCGCG 
CCACTGCTGC TATCCTCGTC GACGAACTCG GGAGCGGGAT CCTCGTTCCG TCTGGCCCGC 
AGCTCCACCC AACCGCTGCA GTCGCCGCGA GTTGTCCACC TGGCCGGTCA GGCTACTGGT 
GGCTCCGCTT TGGCACGCCA GAACTCGGTG AACGACAGCA TCAATCTCAG CGAGCAGCTA 
ACCCTATCCA TCGGCACGGA CAGCGAGCAC ATCTTTGAGA TGTCCGCTTT GGAGGAGGAC 
TCGGCTATTC AGTCGCTGAG CGACGAGACA GCCGCTTCCG CATCTGCCAA CTCAACATCC 
ACGACCACTG CAACCACCAT GACACCGCCC AGTGGCCTGA CGCGGAGCAA CAGCGTTCGG 
GCCAGGGCCA ATATGTTCCA GCAATTGCAG GAGCAGTCGC GCAACCAACG GGCAACGGGA 
ACCACCAACT CCCGTCAATC TCCACCAGCG TCGTCGGCGG AGGTGTCTTC AAGTGCAGCC 
GACAATTCCA TGCACAGTGA CGAGCGGTCC ACGCCAAATG CAACGATAGA TGCGGAATTC 
GAACCCTCAT CCCTGTCGCT GGCTGAGCGA CTGGCCTTCT TCAGCAATCT GTGCGAGAGC 
GGCGGAGGAG GTGGGAGTAC CAGTGCCGGT GGAAGGTACC GCAGTCGCAC CAGCAGCTAT 
TCAAGGAGTC CGCCCGGCGA TCGCACCACT CCCAGGAGCA GCACCACCGC CAGCAGTGTC 
AGTATGACGC CCACGCCCAT GGATTACTCT CCGATTCCCC ACGACAAGGA GCCATCTAGT 
CTCACGCTGG AGAGCATCAT CGAGCCCGAG CAGGAGGAGG TCAAGGAGCA GGTGGACCAG 
GATGAGGTCA AGTTGAGGAA GAAGGAGCCA GTCCAGAGGG AATCTTCCCA GCACAATGGC 
TTCCACGTGC TGACCAGGTC CGCCACCGAA CCGCCGTCGT CCACCTCGCC CCTGCGCATC 
AGCATACGGA CTGTGGGAAA GCTAGTCCTG CCCGATACCT TCCGCGGCGA TCGCAACAAT 
AACAGCAGTA GTAGAAGTGG GAGCAACAGC TGGAGCAACA CTTGCATGGT CAAGCTGCAG 
AGCTTGGAGC CCGTTAGCCT GTCCGTTCAT TCGCGCACCA TTGGTAAGGT TAAGTCGCCA 
TTCATTGAAA AGCAGCAGGA GAAACCACAC ACCGAGAAGC TAGCGAACGG AACCAGCGAC 
AGTGGCTCCG ATTCGGGCAA GGAGAACTGC GCCTCCAACG AGCAGGAACA TCTGCAGCAC 
CAGCAGTCGC AGGACGAGGA TCGAGCTCCG CCCAGGGTGT CCGAGATGCG GAGGAAAATC 
AGCCGACTGG TGCAACAACA GCAGCCGCAA CCACAGCCCG TCAATCGGCG GCACACCACC 
GAGATCACCT CCGTGACCGT TGGAACGCGT TCGCCCAGTG TGGACGATCG CTTTGCCAAG 
TACTTTGGTG TTAAGGAGAG CTGCAACAGC ACGACCACCG TGCATAAAAC GCAATCGCTG 
CCACCCAGCC AAGTTGAGGC CACCAATGCG CACGTGAAAC TACCGCAGAT CACTACCGTG 
GTTTCCAAAC GGCGCGAGCT GCTCAAGAGG ACGCGACCCC TGTCCATGGA TCACTACTCC 
AGCGGCTGCA CCAGTCCCAC AGCCATGCCA AGTCCCAAGA GAGCACATTC GCCCGTCGGC 
CGGCGGAAGG CCGCCATTTC CAGCATCGAG GACATTGAGG TCACGCCGGA TGAACTCCGG 
GCCGCTGGCA GGAACTTCAA GCTGTTGTAC GGCGAGTTAT TGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Svil ( NM_001299990.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286919.1 Drosophila melanogaster supervillin, transcript variant AE (Svil), mRNA.

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CloneID	OFa21974
Clone ID Related Accession (Same CDS sequence)	NM_001299990.1
Accession Version	NM_001299990.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13581bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	supervillin, isoform AE
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTATCTCA TACAGACTGT TCAAACCACT TGGCGGCCAC AATTGCCGCT GCCCAGGGAG 
CTTCAGCTGA CCAATGACCG GAATAGCGCC GACGACCACC ACGATTCAAA GGTGATAACG 
ACCACCACAA CCACAGCGGA CATCCATCAC CACAACCAGC AGGAGGAGAA CGAGGAAGAG 
GATATGGATC GGCTAAGGGG CAATCACAGC ATTGGAGGAT CCTCGCAGCA GCTTTCTGGC 
AAACAGAAAT GGTCCTTCGG GCGACTTTTT CGCAAGAAAA AGGAGGCGGA GAGTGCATCC 
TCCAGCGAAG AAGATCGCAA GGCGGGATTT GTGCCTGCCC AGCGCCAGTC AGCCAGCAAG 
CCGAAGGGAA AACATGGCAA AGGATCGCGA GGTTGCAGCA AATTCGATCA CATTGTGGTG 
AGTCAGCGGC AAGAGCAGGC TCCACCACAT CCACCCCAAC CGCAAATCGA CAATGAGTTC 
TTCTTGCCCT TGGAGGCCAT ACAACCGGAG CCCTACTATC AAAATCAACC GGGCTACTAT 
CAACGATCGA GTAACTCGCT GGACAGGAGA TTACTAATGC AGCCTGGAGT TCAGGGAAAT 
GGTTACCAGC AGCCCAAATC TCGATCGGCG CAGCGAGGTC CCAAGCCACC AGGAGCTCAC 
TCCAGCGAGG AGGAACTGAT CTCGCTGAAC TCCTCCACGT TCTCCAAATA CCGCAGCGAT 
GAGAGTATTC ATTTGGGTTC CGCTGCTGGA CAAAGTCGAC GCAGTCGAGC GGCCCGCAAT 
GAGCGTTACT ACAAGAGATT GTCCCGGGAT GGCGAACCGA GTCATGGCGG AGCACCCGTT 
GTAGCCCAGC AGCGATACAA AACCCAGCCA CTGCCTTTAT CCATTTACCA GCCAACGAAC 
GCGTCCACTG GCTATGTCCA GTGGCAACCG CAGCCCCAGA AGAACCTGCA GAATGCGATG 
CAGAACGATG GAAAGCGAAG CATCAGCTAC GACAGTCAAA TACACCTGCA GAACATGAAT 
GGGAGGATGC AGAGTAAGCC ATTGCCACCG CCACCGCCTC CAAGGGATCC ACTGAGGAGA 
GTGCATGTTG GAGGTTCCGG GAAGATTGGA TCCAGCTCTG GAGACTTGCG TCCCGTGTCC 
TATGCCTTCG ATCACAATAG TCGCTGTGCG TCGGATGATC GCATTTGGCA GCCACCCCAC 
TACCAGTCGG TGCATTCGCT CAACTCCCAG CCCAGTTCGT CAATAGTCAG TGCACCAGTG 
AGTCAACAGC AACCGCACCA CAGGAGATTC ATAACTCGAT CCGAGAGGAA CGCAAATCCT 
GGCAAGCAAT CCCTGCCGAA TGGTATTGAT TTCCATTATG TGGCAGATGC AACGCCACGC 
TCCAGGAAGC CCATACACAT GATGGATGCA GGCAGGATGG ACAATACAGG AGTGCCAGCA 
TCCAGTGGTA TATTGCGAAC CTCTAAGAGT GGGCTGCCCA CACCAAGTCC TGCTCCCCAG 
CAGCCCTTGG CCAAACCGCG GTCGTTCTCC AGCTCGCGAC TTAGTGAGCT GCGAACCTAT 
CCCATGCCCA TGTACTCCGA GGTGCAGAAA CCTAAGAGGA ATGCGCCACC AGTCAATCCC 
TCGCCGAATC AGGATAACAT AGTGTGTGGG AGTCTGCACA TAAAACCCAC ACAAGAGCAT 
GGAAATCAAA ACTATGTGGA GATTCGTCAT CGGGATGTGC ACAAGACCAA CTCAATGCCG 
CACCATTATC AGAATAGGCG ATCTGGCGAG GATTTACCCA ACAAATATGA GGAGTACGTG 
GCAGAAAAGA GGGAGCAGCA TCAACAGGCA CCACCCCCAG TTTATCCGGA GCGCAAAATG 
AGCTTGACAC CAGCAGTGGC GCCACCGCCT CCTGTTTACT TCCCTCGCAA GAAGCCTGCC 
AATTTGGAGG AGGCCATCAA CGAACTGGAG GCCATTTACA AGTCTCTAGG ACTCACTGAA 
CCAGCCGAGC CCAAACAGGA ACCAAAGCTG GATATTTCCT CCAAGATGCG TGTGCCAACT 
CCCAGTGATT TTGAAAAGTT CGCCTTGGCG CATGCTGATG ACTACGAGGA TGAAGATTCG 
CCAACCGGAG AACCAGATCC AATCAGAGAT GATGTGGCCT TCAGGAACCT ACAGTTGGCC 
AATCTGCAAC ATCGGCCATC GGAGAAGCAG CCTCCATTCG GCATCCCAGT GGGACCCATT 
GTGCCAGCTC CACAGTCGGA TTATCTTCAC GTAGAGCCAG TGAGGGTGAA GAAGAAGAGT 
GGCTCTCCTG ACATCATTAA AGATGATCTT GCTGTGCGTT CATTGAGAAA GGATCCTCCT 
GGTCCCAAAT CCAGCTTTGT CTACCCAATG CAAAAGAAGC AACGGGCTAT TCGAACCCAA 
TCGGCCAACA TATACAATCT GATCCAAAGA GACGCTGCCA AGCCATCTGG TGGAGATCTT 
AAGAGCTACA TGGAGCTGAC TCGCAACCTG GAAAGAGCTG GCAGCATGTC CAATCTGCAG 
GGTGAGGATG GCAGTGATAC CAAAGATGTA CCCGCCACAC TGGATCTTCT CCGAAAGTTG 
AAAGCTCAGG ATGAGGAACT GGATTTGGCT AGAAAGCATC ACCCCATACC GTTTAGACAT 
CCCAGTCAGG GAGGAGCTAT TGCCCAACTG CCAGAACGGT TGTCCAAAGA GGAAATACCC 
AAGGCAGCGC CTGTGCCAGC TCCCCGAAAG AGTCTGACTC CTGAACCCAT GCTAGATGAT 
GCTCTCAACA AGATTGCCCA GGATGCGCAG GCCAGCAGCA TTAAGCTGAG TCAGGAACTC 
CATGAACTTC GCAAGGAGGC ATTGATTACA GCTGCGAGAC CAAAGTTGAA TGCCGAACAA 
CAGAGATTAG AGGATGAACT GCAGCAGATA GAAGCTGTCT CCGAGGCAGC CAAACGTTGT 
GGCCAAATGC TGCTCGAAAC TCTGCCAGAT GCGGGACAGG AGGGTCAGGA ACCTCAGGAT 
AAGCCCCTCA GGAAGCTACA TAAGGAGGGC AAACTCATAC GAGCTATCGA TGAGGTGTCC 
GAAGCTGCTA ATGCGGTCTG TGAGAAGATC CTCAAGGACA TTGTGACCAC AGAACCACCG 
GTGGTAGTTC AGGAGGCCCT CCAAGTGAGG CAGACCACAA AGACTGGACC AGGCGGAGAA 
CAGCTGGTGA TGCCGCATCT TATCAAGAAA CTAGATCCCA TACAATCCGA CAAGATCGAG 
CACATTGCCA GACGATGCAT GCGGCAGTTG AGCGAACTGG CCAATAATCC GGACTACGAC 
AACCTGGCCA CCTGTAATAT GAGCAACAGC ACCACCAACG CAACGGGTGG AGCAGCCGCT 
GATGCCACTC TCGTTTGTCC CGTTCCAGAG ACCCAAGCCA AGAGCCAGAC GCTCGAGGAG 
ATTGACAAGA TCATGCAGGA GTGCGAGCGT CAGGCGAAGA GCAGCAGCTC AACCACGACC 
ACCGATGATC AGCGCACCAC TGCCCCCAGT CTGACGAGTG GAAGTGGCAG CGGGAGCTTC 
CCACCCAATG TGAGCACCAC CGCCAGTTCG TTCAGCTCAA GCAGCGACTG TTTGGCCAAG 
TCGTCGAGTC CTTCGCGCGC CAGCCGTCCG CTCTCCTCGA GCATCACCTC TTTCAATCCA 
TATTCCTCCA GCGACTACAT CAAGTCGCAG AGCAGTGATT GCCATGCACC AAGCACCGAT 
CCGATCAAGA CCTTTAGCAC CACCTCGTAC GAGGTGCAGT CCACACCGCA GAGCACCACC 
ATTAGCACCA ATACGATGAG CACGAGCCAG AGCAACACGA TCAGTAGCAC TACTAATCCG 
AGTGTGGAGT CCAAGCCATC CGCCCCGTCG GTATCGCCAC CACCCCTCCA GTTGACGCCA 
GTGGGTGATC GGAGGGGCAG GAACCGGGAG CGGGGAGCTT CCTGCTCCTC CACATCCTCA 
CCTTCACAGT ACAACTCGAG CGAGGAGCTG GCCGCCATTT TTGGCATCGA GGAGCAACCG 
AAACAGCAGC GTCGCAGTCG GCAGACGCAT AATGCCACAG AAACCATTGC GGCCTCAAGT 
GACTTATCCA CAGCACCTGC CACTGCCAAG ACCAGCTGCC AGCCACCTGA GCCACTGAAA 
CAGAAGCCAC CGCAGCAGCA GACCACCAAG GACTCGGAGG ATTCCAGTTC ACAGAAGAGT 
GCTCCAGTCC AGTCGTATCG TGGTCAGCAT CCGCACTTCC ACCATCACAC CACGCCCGCT 
TACTACACGG ATCTTCAGCT GAGAATTCAC ACGCCGAACG CATCGGATGT GGGCGCCTTG 
AACGAGAACT ATCGAAGTGT ATACAGTACT CCGAGCACCC AGCTGGTGGA ACAGCGATCG 
TTTCCAAAGA GCTCCACGGC CACGAAGGAT CCACCGCTGC CCATAAGCAA TCCGGCTAGG 
GGAACATTTT TTCGTGGCGA CTCTGCGTCC AGCGACAGTC ACTCAAGACT CCGTCGGAGC 
CAGCAGAGAA CCGGCAGCGA GGGACGCTTC TTCAGATACT CTCAGGGATC TGTGAGCCCT 
ACCAAAGGTG AACCTCCAAT CGGCCACGAA GGTGGTGAGC AGCCACCCGT TGTCGCCAGT 
CACTCCCCCA TCACATCCAC CAGCACCACC ACCATCAGTA GTTGTAACCC CGTAGAGCAG 
GCTAGTCCAC CACCAACAAA CGTAGTAAAT CCCAGCGGAT CCGGTCGCAA CAGCCGGCAC 
AAGCAGCGTT TCCACAGTCG ATCCCGTCGA GCGGTGCGCG TGCGCAGTGA ATCGCGTCCG 
ATTAGCGCCC TCTATGACAT AATATGCAAG GAGAAGAACC TGGAAAACAG CAGCAGCAGC 
AGCAGCAGCA GCAACGAGCA GGAGAAGAAA AAGGATCAGG AGCAGCCTAA GACCACAACC 
GACAAGCTGG CGACCTTTTG GCCCCTGCAC CATCACTACC TCAATCCCCC GATGAGCAGC 
GGCAACTCGG GCGGCAGCAG TGGCAATCCC AAGCAGCAGC AGCAGCAGCA GCAGCAACAA 
CAGCTAGAGC AGCTGGGACA CCAGACACAT CTTGTGCTAG GAGCATCAAA GGAGTTGCCC 
GCGGTCAGCA GCAGCAGCGA TGAAGAGGGA TCCCTGCGGA CTCTCCACTC CAGTCGCAAG 
GTGACCAATC TGAATGCCAA GTCCACCTCT CTGCCACCCG AGGAAGGACT CTCGGGTGAT 
GTCTCCCTGG AGTCGTCCTC CAACCGACTG AATCTGCGAT TGGCCAGGAG CCTGGAGCCC 
TCAGTCCGCA ATATCACTGC ATCCGCCTCC AAGGAAGTAC AGAGCTATGA TGCTGGGGAG 
CCTGTGAATA CCAATCGATT GTTTGTGGAA CCGCCCAAGA TACCACTGTC TGCCATCCGA 
AACTCTCTTG ACGATCACGA CCTGGATATT GAGGAGTGCG ACAGCACCAC CCAGTTCGAT 
CAGGGGGATA TCTACGATAC CCTGGAACGC TGGAGCCATG GTGTCAGCTT GGGGGCACAT 
CTATTGAACT GCGCGAATGG CCACGATCAA TCTGATCCCG AGGACGACCA TCAAACCGAT 
CATCATCAGC ATGCACATCA ATCATTGCCA GTAGAACCCA AGTCTCATCA TCATCAGCAC 
CACCAGGCCA CCAATGCGCG CAGATTCATA ACGCGCACCA CGCGTTCCGC CAGTCGCCAG 
AGTTCCGCCG GACACTCCCC ACCTCCGCCG CTGCATCGCA GTGACGACTC CTCCAGCAGT 
TCACCCTCAC CGACACGCAG ACGGAATAAG CCAGGTGGTG CTCCTGCCGC CACAGATCCA 
CCATCATCGT CGGCGAACGG GAATAGTTCC GCTTATGAGA CGGCAGCCAC CACTGTGATG 
ACCCATGACC AGCAGCGAGA TCCTCCAGTT GAAGTTCCTA AGGCTCTAAA CGTCGAGCCA 
CCGCAGCCAG AAGACATCGT CCTGGGCTCC TGCCCCACTG CATCTGCCCA CTCAGCAGAG 
AGCCAAAGTC GAGGAAAAGC TGGCAAATGC AGTATTAATT GCACCTACAG CCACAGCCAC 
AGCACCAACA ACAACTGCAA CGCGCGACAA CGCAACAGCA GCAACATCAG CAGCAACAAC 
ACCATCAGCA GCAACAGCAG CAGCAGCAAA AGCACTTGCA ACAACAATAG CAAACAGCAA 
CACGCCACGC CCATGCCGCC CCTAGCTAAT GCCAACAAAG CCCCGTTAAT TGTGCTTAAG 
ACAGCCGCAT CTGCAGCAGC ATTGCCGTCC ATTGCCGCAG CAGCAACATC CAGCGGCACG 
GCAGCAACAT CTGGTGGTGT TGGTTCGCCC ATCAAGCGTC GCAAGAATCC GCTGTCGCCG 
CTGCTCCTCG GCAGCCAGTG CCAGTCCACC ATTGCGCCTC CGCAGGCCAT TGCCAGTCCG 
CTCCTGAGCG GCGGCAGCTG CTCCTCCTCG ACCACCTCGC CCCAATCCTC GCCAGCGGTT 
CGCACCACCA AGGCGAGTCG TCTGCGCGCC GCCGCGCTTG ATAAAAAGAA GGAAGAGGAG 
CAACGCAATC GCTACAGTTC GCCCGTTTCA CCAAAAGCCT CCAGCAACGG CGGAGTCCAA 
GCACAACGTC GTCTTAGCAA CTCGGAGCTA TCGCCCAAGT CGCCAGGTGC CAGTGGATTC 
ACTTCGGAGC CAGTCGCGTC AGCCACAGCG AACAGAGTCC CGACGAGATC TGCCTCCCAT 
CCTGTGGACA CACCGCCCAC ACAAAGGCGA CAGCCTCTGG TGGCCGTGAG TTCGGGCATC 
TCCATGAAGC AGAGTCTCAG CGAGCTGCAC TTCACGGGTG GAGCAGTCAG TCCGCCCAAG 
GCTACGGCGG AATGCACTCT AAGGATGCGA CCACGTAGCT CCACGATGAG CACCGAGAAC 
ACGGAGAATG GCCGACGTGA CCACTTGGCT AATCTGCAAC TAAGTCCTGT GCAAAAGCAA 
AAGCGAACGC CCGAAGTATC ACCACGCCGT TTCGGAGATC GCGACAAGTC GGCCAAACGG 
AAATCGAATT TGAATCGTTC GTTGACCGAG GAAGCCACCA AGGATGGCGA TGAGGTTGCC 
TCTTCGACCC GCCGGCGCCG ACGTCGTGAA CTGGGAATGG CTCGTCCCCT GGTTCCGGGC 
GATGATGACG CCCTAAGAGA TTTGCTAACG CCGCAGAAGA GTTCCTGCAG CGAGTCAACC 
AACTCCATAT CCGAACCCCA TCTCCAGTTC ATACCAGCCG TTCGTCATCC GGTGGTTCCA 
GTACGCACCA TGACTGCTGT GCTGCCTGCA CCACGACCGC TGCATACTCC TGCGGCCCGC 
AACGTCAGCC AGAAGTTCAA TGAAAGGACC AAAACAGATC TGGCCGAGAT CAAGAAGATT 
GCTGAGCAAT CGGAGGCGCA GGTGCAACAG CCTTCGGTGG ATGATAACCA GAAAATGTCA 
GCCATAATGC GCAGGAAGAG CACGGATGAG GGTGGATCAG CTTTGGGGGC AACAAACCAG 
AATTCACCAC CTGCCCCAAA CCAAATTGTA TCCATACTGA AGAAGAAGGA ACATGGACTT 
GGCGAGTGCA ACTCCAGTGC TTCAAGCAAT CCCTCGCCGG TGACCTTCTC TTCTAGCGTG 
GTGGATCATC CTTTGGCTGG AGCTGGCCGG TGCAAACGGC AGGGAATACT CAAGAAGAGA 
TCGAGTCTAG ATGAATCGCG CTACTACTCC CGATCGCATT CACCCGACGA ACGTAGTATT 
CTCATTAAGT CGGCCAGGAG GAATTCCCTG GAGGAAGCTG GCACTGGATT GAGTCCGGCT 
CAGGCCCATG GAATCCTCAA GCAGAGCAGC TACGACAGCA GCAAGAGCGA CGGCTGCCCT 
TCGGCCACGG AGAGCCAGCC ACACAGTATC CTGAAAAAGA AGGATTCGCT ATCGACACCT 
TCGGACGGAG GATGCCACAA GCATGTCTCT ATATCGCAAG CTGTAACTTT AGCTGCTGCT 
GAACTGGCTG CCCATGATGG AATCACCTTC GATGATGGTG AGGAGCATGA GATACGACCG 
ATCCTAAAGC AGGAGAGCAC CTCCAGCGAG GAGGCTGTGC GTCCGCCAAA GCCCATACTC 
AAAAAGAAAT CCTTTGGCGA GGCGGATGAA CACGAAATAC GTCCTATATT GAAGAGCTCT 
CGTAAAAGCA GTCGAGAGGA GTTCGATTTG AGTGGCTTGG AAAACGAGGT CAACGATTCC 
CTATCGTCAA TCCTAAAGAC AGACTCGCCG TCCAAGCGTC GGTCGCTCGG TTCAGCTTTG 
CATGATTTGG AGGAGTCCAC CACTTCCCCG AGCATGCTCA AGAGGCGCAC CCGTTCGCTG 
GAACGTCAAG ATGTCCAGCC TGGCATGGAT TTGGCAGCTG CACTGGATGC CATCGCCACA 
TCACAGGCTA CTCCCAGCAC CCCGGTGGAC TTCGTGACCA CACCAACAAG TATATCGGTG 
GCGGAGCGGA TCAAGCGCAT GGAGATGCTG TCGACGCCCA CTTCCCGTGC GGCGGGTGGG 
GCCAACAGCA GCTGGGAATC GCCACTGCAG GCATCGTCAG CAGAGCATGG AGCACCCACC 
ACCCCAAGGG TTCTCAAGCC AAGTGTGCTG CGCAGAGATT TGCAGAGGGA GCGGTACAAA 
ACGCAACCCG TGACCAACGA GGAGATGAGC TTCTTCAAAG CCAACTCCGC GCCCTTCGAC 
ATCTCGGCTA CATCGGCCTT CAAGCCCACC AAGCCGCTGG ACATCCGCTT GACCCACGCA 
CCGCATGCAC CGCTGATTTC GCCGATCACC GGACAGCCAC TCAGCCACGT GCCCGCGCCA 
CTGCTGCTAT CCTCGTCGAC GAACTCGGGA GCGGGATCCT CGTTCCGTCT GGCCCGCAGC 
TCCACCCAAC CGCTGCAGTC GCCGCGAGTT GTCCACCTGG CCGGTCAGGC TACTGGTGGC 
TCCGCTTTGG CACGCCAGAA CTCGGTGAAC GACAGCATCA ATCTCAGCGA GCAGCTAACC 
CTATCCATCG GCACGGACAG CGAGCACATC TTTGAGATGT CCGCTTTGGA GGAGGACTCG 
GCTATTCAGT CGCTGAGCGA CGAGACAGCC GCTTCCGCAT CTGCCAACTC AACATCCACG 
ACCACTGCAA CCACCATGAC ACCGCCCAGT GGCCTGACGC GGAGCAACAG CGTTCGGGCC 
AGGGCCAATA TGTTCCAGCA ATTGCAGGAG CAGTCGCGCA ACCAACGGGC AACGGGAACC 
ACCAACTCCC GTCAATCTCC ACCAGCGTCG TCGGCGGAGG TGTCTTCAAG TGCAGCCGAC 
AATTCCATGC ACAGTGACGA GCGGTCCACG CCAAATGCAA CGATAGATGC GGAATTCGGA 
AAACCCAGCA CCGAACCACT TAAGAGCATT CTTAAGACGG GAATGCCAGT GATGGGTATG 
GGACGCGGTT TAATGCCTGT GGATCTCAAT GCCGAGCTGA AGAACCGGCT CAAACGCTCC 
ACACATGCCA CTGTTTCCAA TCTCCGCAAA TCTGCCACCT CGGCAGATGC CACTAGAGCA 
GCTAGTGATG AGGTGGACAA TCCGCAGAGG AATTTAGCCC AGATCCTGAG GAATGTTTCC 
AAGGAGAACT CCACGCCGAA GCACGATGAT GCCGTGAGCC TAGTGCGGAA TCTCTCTACT 
TTGGGACAAC CTCGATCGCT GGCCCGTGAC GATGCAGCCG GGAATTGTGC CTCGGCATCC 
GAAGGCGAGA GTTCGGGGGG CCGCGAGATC GAGGCCATAA TCAAGAACAG CGCTGTGGCC 
AGACGTCGCC GGCAGCAACA GCAGCAGTCG CAGCAGCAGA ATCAGCCAGA TGGCAATCTC 
GTTGCGAAAA GCAAAAGTCA CTCGGGCATA TCAGCACCCA TCGGCCTTGG CCCCCAGCTA 
CCACCCCTTC CCCTCGGCGG CGGTTTTGTT AAGTTGCGTC ATGTAGCCAA GGAGGAGGAT 
ACCAGTAGCA GCAGCAACAC ATCCTCCGCC AAGGAATCGC CAGATAAGAA GGATCTTCGC 
CAGGAGCAGG CAGAAGCTTC GGGAGACTCA TCGCACCCAA CGGCTCTGCC ACAGTTTCTT 
CAAGGCGTCC AACGATCATC AACCGAGGTC ATGGGCCCCG ATCAGCGGTC TTTGGTGATT 
TCGAAGACCG GCTCCATTGC GGAGCGTTTG GCGGCACTCC ACAAAAGTGG CGAGGACGAC 
TGGAAGAAGC GCATCTCGAA GCGCGACGAA GTCGACGATG TGCACCGTGA AAACTTCGTA 
AACGAATCGC TATCTTTGGC CCACTCGCTG TCGGACAAAC CACTGCCCCC ATCACCTTTG 
ATACCCACCA GCTTGGAGGG CGGCAAGGTC TCCGATCGTT TGAGCAAACT CAAGTGCAAC 
TCGGAGAACT GGAAGCAGCG CATAGAGCAA ACCGATGCCA AGAAATTCAC AGTCGCCGGT 
CGACTGCAAA AGAAGGCCCA ATCCCCCGTG GAGCTTCAAT TCGAGCGTTC TGCCAATGAT 
GCGGCCAAAA AGTGTCCCAT GCTGGAGGTG CGCAGCGCCA ATCAGCCGCA GCTTGGGCTG 
GCCAAGAGCC CCTCCATGAT GGTCACCTCT AGCACCAGCA CCAATAGCAC CAGCAAGTCC 
GCACTCCGCA GCCTGAGCGT CAATCCCGAG GAGGAGGGAC CCACCAGCCT GAGTCGCTCC 
AACAGCAGCA GTTCGGAGTC CGATGGAGAG CGCAAATCCC CCCAGAATGC CAAGCAGAAC 
AAGGAGCTGG CCAACAACAA GAGTGCTGCA GCACCCACCA ATGTGGGAGC CAGGATTCAG 
GTGCCCCGGC TGGACGACAA GGAAACTTTC GAGAACTTCT TCTCCTCCAA GCCGAAGAAG 
GAGGAGAACG TTCAGCTGGA GATCAGCGCT TTCGATGACA TCAAGCCCAC TGAGCGCTTG 
GTAAGCAAGC GACACGTACA GGGTCCCAAA GGTCGACGGG CTGCACGCAA TCCTCTCAAG 
AGTTTAGCTG CTCGTGAGGA CATTAGCTCC GAGTACACCG AAATGAGGTC GGGCATAGCC 
GAGCGAGAAT TACGGCGCCT AAAGTTGGAG TCATATGGTC ATAATGCCAA CTTGGCTGCA 
GAGGCCATAG CCGGATTAGC CTCCGTCGAG GACTTCAAGT CGGTGGCACT GCGCTCCTCG 
TCGATTCCAC TGCAGCAAAT GTGGCTACCC CACAAGCCAG TGATGTTACT CCATGTTAAG 
GGGCGCACAC ATGTCCAAAC CCGACTGGTG GAACCCGTGC ATACCTCCCT GAACCGTGGT 
GATTGCTTCA TCCTGGTGGC CGGATCGCAG CTTTTCCGCT ACGTTGGATC CTTTGCAAAC 
GTAATCGAGA TCTCCCGGAG CAAGAAGATC TGCGCTGCCA TAGTGGAAAA CAAGGATCTC 
GGTTGCACCG CCACCCAGGA GGTGATCCTC ACGGATGGCA AGTATTTGAA GGAGCGCCAG 
TGGCGTCAGT TCTGGCAGCT TTTGGGCAAA AAAGAGGATG ATCAATCGGA TATAGCTGAA 
TGCGGTCATG CCGATGAAGA CGACGTGTTC GAGAGCAGCC TGATCGAGAC TAACAAGATC 
TACGAGTACC AGGATGAGGC GCTCATTCCG TTGGAGAAAT TCTGGGGCTG CATCCCCAAG 
GTGGACATGT TGGACACGCG TAAAGTTTTG GTCTTTGACT TTGGCAGCGA GCTGTATGTG 
TGGAATGGCA AGAATGCCCC TTCCGATGCC AAGCGAGCTG CCATGAGATT GGCCCAGGAA 
CACTTTGGTG CTGAGAATTC GGCGGACTAT GCCGACTGTT ACTTGAATCC CCTGAACTAT 
GTCGCCATAG TGGGTCGTCG TGAGAACACC AAGTATGCCA AGAGGTGCGC ACAGCGTCCA 
GAATGGTGTG TTCTCGGCAA GATCACCCAG AACATGGAGA CGGTGCTGTT CAAGGAGAAG 
TTCAGTGATT GGCCAGAGAT AGAACGCGAG GATCTGGAGA AGGATTACCT ATCAAACGGA 
GTGCATATGG TGCGTGCTTT AAACGGCACC GCTTTGCATA AGGGAGAACC CTACCAGGAA 
CCAAATCTGG TGCTCGAGCA GGCCAACTTG GGGCGTGGCA ATTTCTACTA CGACACGGAC 
ACCATGCGGC ACTTCGATGT GATTACCAAG TCAACGGACA AATGGCAGAT CCACGAGTTC 
AACTTTGATG CGGCGAATAA TCGCGATGAC TACGGTCACT TCTACTCGGC GGAGAGCTAT 
ATAGTGCGCT GGATCTACCA GATTTCGGTC ACAGTTCGCG AACTCAGTGG CAAGGTGTCG 
AACAGAAGCA CAGTGGGTCG AGACAGGTGT GTCTATTTCA CCTGGCAAGG ACAGGACTCA 
AGTGCCAACG AGAAGGGTGC AGCTGCTTTG CTGACCGTAG AGTTGGACAA GGAGAAAGGC 
GCACAGATGC GGGTGTCGCA AGGAGATGAG TGCACAGCCT TCGTCCGCCT GTTCCGGCAA 
ATGTGGCAAC ATCGTGGACG CAAGGAGCAA TGTCTGGAGA GGCACAGCGA GTGGAGGCTC 
TACCAACTGC AGGGCAACGT CAGCGAGGAG ACCATCATCA AGGAAGTCGT CTGCCAGTCC 
AGCCATTTGC GATCCCGCAG CTCCATGCTG TTAATTCACG GCAGTCAGGG AAATGTGATT 
GTCTGGCATG GATCGAAGAG TGCTCCTCAT ACGCGTAATG TGGCTGTAGG AGTTGCTCAG 
GATCTGATCA AGACGGTGCC TAAAGATCTC TTCAGCGCCG AAACTGCCAA CCTAACTGAG 
GTGGAGGAGG GTTCCGACGA CGATCAGTGC AAGAAGGCCC TGGGATTGAA GGATGAAAGA 
AATGAATATG GCAGCCTGCT TAAGTCCACC AAGTCGTTCG ATTACCAAAT AAGGATCTTC 
AACTTCTCCA GCACTCAGGG TGTTTTCAAA GCCCTCGAAC TGAGCGATCC GCTGCGCTGC 
CAGGATCTGC ATAGTCCGTA TCCCTTCAGC CAGTCGCAGC TGTACAATGC AAGGCAGCCG 
ACTATCTTTT TGCTGGACGA CGGCGATGAG TTGTGGCTCT GGATGGGATG GTGGCCTCTG 
GAAGATGTGA AGATAAACAC CGATGAGCGA AGCAGTCCCA CCAACGAAAA TCGCGCTGGA 
GTAAATCGCT GGATCTCAGA GAGACGTGCT GCCCTCGAGA CAGCCGTTGA CTATTGGCGT 
GCCAAGCACG GCGAGAACGA GAATGAACCC TTCCATGGCA TCAAAGGCCA GGTTGTGTGG 
GCAGGCCTGG AGCCACTGGC CTTTAAGGCG CTGTTTCCCG ACTGGACAGA ACGTCCGGAT 
GTAAGAGAAA TCAACGAAGA AGATGGGCGA ACTGATGCTT CCATATCGAT CGCCACAATG 
CTGGCACAAA TGACGCAGAC CGAGTATCCC CTGGAAGTCC TGAAAGCCCG ACCGCTGCCC 
GAAGGTGTGG AGCCCACCCG CCTGGAGGTC TACCTTAATG CGGAGGACTT CCAGGCGGCA 
CTGGGATGCA GCCGGGCGGA ATTCGAGCAG CTGCCCATCT GGAAACAGAC CAAGCTGAAG 
AAGGAGCGAG GGCTGTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286919.1
CDS	776..14356
Translation	MYLIQTVQTTWRPQLPLPRELQLTNDRNSADDHHDSKVITTTTTTADIHHHNQQEENEEEDMDRLRGNHSIGGSSQQLSGKQKWSFGRLFRKKKEAESASSSEEDRKAGFVPAQRQSASKPKGKHGKGSRGCSKFDHIVVSQRQEQAPPHPPQPQIDNEFFLPLEAIQPEPYYQNQPGYYQRSSNSLDRRLLMQPGVQGNGYQQPKSRSAQRGPKPPGAHSSEEELISLNSSTFSKYRSDESIHLGSAAGQSRRSRAARNERYYKRLSRDGEPSHGGAPVVAQQRYKTQPLPLSIYQPTNASTGYVQWQPQPQKNLQNAMQNDGKRSISYDSQIHLQNMNGRMQSKPLPPPPPPRDPLRRVHVGGSGKIGSSSGDLRPVSYAFDHNSRCASDDRIWQPPHYQSVHSLNSQPSSSIVSAPVSQQQPHHRRFITRSERNANPGKQSLPNGIDFHYVADATPRSRKPIHMMDAGRMDNTGVPASSGILRTSKSGLPTPSPAPQQPLAKPRSFSSSRLSELRTYPMPMYSEVQKPKRNAPPVNPSPNQDNIVCGSLHIKPTQEHGNQNYVEIRHRDVHKTNSMPHHYQNRRSGEDLPNKYEEYVAEKREQHQQAPPPVYPERKMSLTPAVAPPPPVYFPRKKPANLEEAINELEAIYKSLGLTEPAEPKQEPKLDISSKMRVPTPSDFEKFALAHADDYEDEDSPTGEPDPIRDDVAFRNLQLANLQHRPSEKQPPFGIPVGPIVPAPQSDYLHVEPVRVKKKSGSPDIIKDDLAVRSLRKDPPGPKSSFVYPMQKKQRAIRTQSANIYNLIQRDAAKPSGGDLKSYMELTRNLERAGSMSNLQGEDGSDTKDVPATLDLLRKLKAQDEELDLARKHHPIPFRHPSQGGAIAQLPERLSKEEIPKAAPVPAPRKSLTPEPMLDDALNKIAQDAQASSIKLSQELHELRKEALITAARPKLNAEQQRLEDELQQIEAVSEAAKRCGQMLLETLPDAGQEGQEPQDKPLRKLHKEGKLIRAIDEVSEAANAVCEKILKDIVTTEPPVVVQEALQVRQTTKTGPGGEQLVMPHLIKKLDPIQSDKIEHIARRCMRQLSELANNPDYDNLATCNMSNSTTNATGGAAADATLVCPVPETQAKSQTLEEIDKIMQECERQAKSSSSTTTTDDQRTTAPSLTSGSGSGSFPPNVSTTASSFSSSSDCLAKSSSPSRASRPLSSSITSFNPYSSSDYIKSQSSDCHAPSTDPIKTFSTTSYEVQSTPQSTTISTNTMSTSQSNTISSTTNPSVESKPSAPSVSPPPLQLTPVGDRRGRNRERGASCSSTSSPSQYNSSEELAAIFGIEEQPKQQRRSRQTHNATETIAASSDLSTAPATAKTSCQPPEPLKQKPPQQQTTKDSEDSSSQKSAPVQSYRGQHPHFHHHTTPAYYTDLQLRIHTPNASDVGALNENYRSVYSTPSTQLVEQRSFPKSSTATKDPPLPISNPARGTFFRGDSASSDSHSRLRRSQQRTGSEGRFFRYSQGSVSPTKGEPPIGHEGGEQPPVVASHSPITSTSTTTISSCNPVEQASPPPTNVVNPSGSGRNSRHKQRFHSRSRRAVRVRSESRPISALYDIICKEKNLENSSSSSSSSNEQEKKKDQEQPKTTTDKLATFWPLHHHYLNPPMSSGNSGGSSGNPKQQQQQQQQQQLEQLGHQTHLVLGASKELPAVSSSSDEEGSLRTLHSSRKVTNLNAKSTSLPPEEGLSGDVSLESSSNRLNLRLARSLEPSVRNITASASKEVQSYDAGEPVNTNRLFVEPPKIPLSAIRNSLDDHDLDIEECDSTTQFDQGDIYDTLERWSHGVSLGAHLLNCANGHDQSDPEDDHQTDHHQHAHQSLPVEPKSHHHQHHQATNARRFITRTTRSASRQSSAGHSPPPPLHRSDDSSSSSPSPTRRRNKPGGAPAATDPPSSSANGNSSAYETAATTVMTHDQQRDPPVEVPKALNVEPPQPEDIVLGSCPTASAHSAESQSRGKAGKCSINCTYSHSHSTNNNCNARQRNSSNISSNNTISSNSSSSKSTCNNNSKQQHATPMPPLANANKAPLIVLKTAASAAALPSIAAAATSSGTAATSGGVGSPIKRRKNPLSPLLLGSQCQSTIAPPQAIASPLLSGGSCSSSTTSPQSSPAVRTTKASRLRAAALDKKKEEEQRNRYSSPVSPKASSNGGVQAQRRLSNSELSPKSPGASGFTSEPVASATANRVPTRSASHPVDTPPTQRRQPLVAVSSGISMKQSLSELHFTGGAVSPPKATAECTLRMRPRSSTMSTENTENGRRDHLANLQLSPVQKQKRTPEVSPRRFGDRDKSAKRKSNLNRSLTEEATKDGDEVASSTRRRRRRELGMARPLVPGDDDALRDLLTPQKSSCSESTNSISEPHLQFIPAVRHPVVPVRTMTAVLPAPRPLHTPAARNVSQKFNERTKTDLAEIKKIAEQSEAQVQQPSVDDNQKMSAIMRRKSTDEGGSALGATNQNSPPAPNQIVSILKKKEHGLGECNSSASSNPSPVTFSSSVVDHPLAGAGRCKRQGILKKRSSLDESRYYSRSHSPDERSILIKSARRNSLEEAGTGLSPAQAHGILKQSSYDSSKSDGCPSATESQPHSILKKKDSLSTPSDGGCHKHVSISQAVTLAAAELAAHDGITFDDGEEHEIRPILKQESTSSEEAVRPPKPILKKKSFGEADEHEIRPILKSSRKSSREEFDLSGLENEVNDSLSSILKTDSPSKRRSLGSALHDLEESTTSPSMLKRRTRSLERQDVQPGMDLAAALDAIATSQATPSTPVDFVTTPTSISVAERIKRMEMLSTPTSRAAGGANSSWESPLQASSAEHGAPTTPRVLKPSVLRRDLQRERYKTQPVTNEEMSFFKANSAPFDISATSAFKPTKPLDIRLTHAPHAPLISPITGQPLSHVPAPLLLSSSTNSGAGSSFRLARSSTQPLQSPRVVHLAGQATGGSALARQNSVNDSINLSEQLTLSIGTDSEHIFEMSALEEDSAIQSLSDETAASASANSTSTTTATTMTPPSGLTRSNSVRARANMFQQLQEQSRNQRATGTTNSRQSPPASSAEVSSSAADNSMHSDERSTPNATIDAEFGKPSTEPLKSILKTGMPVMGMGRGLMPVDLNAELKNRLKRSTHATVSNLRKSATSADATRAASDEVDNPQRNLAQILRNVSKENSTPKHDDAVSLVRNLSTLGQPRSLARDDAAGNCASASEGESSGGREIEAIIKNSAVARRRRQQQQQSQQQNQPDGNLVAKSKSHSGISAPIGLGPQLPPLPLGGGFVKLRHVAKEEDTSSSSNTSSAKESPDKKDLRQEQAEASGDSSHPTALPQFLQGVQRSSTEVMGPDQRSLVISKTGSIAERLAALHKSGEDDWKKRISKRDEVDDVHRENFVNESLSLAHSLSDKPLPPSPLIPTSLEGGKVSDRLSKLKCNSENWKQRIEQTDAKKFTVAGRLQKKAQSPVELQFERSANDAAKKCPMLEVRSANQPQLGLAKSPSMMVTSSTSTNSTSKSALRSLSVNPEEEGPTSLSRSNSSSSESDGERKSPQNAKQNKELANNKSAAAPTNVGARIQVPRLDDKETFENFFSSKPKKEENVQLEISAFDDIKPTERLVSKRHVQGPKGRRAARNPLKSLAAREDISSEYTEMRSGIAERELRRLKLESYGHNANLAAEAIAGLASVEDFKSVALRSSSIPLQQMWLPHKPVMLLHVKGRTHVQTRLVEPVHTSLNRGDCFILVAGSQLFRYVGSFANVIEISRSKKICAAIVENKDLGCTATQEVILTDGKYLKERQWRQFWQLLGKKEDDQSDIAECGHADEDDVFESSLIETNKIYEYQDEALIPLEKFWGCIPKVDMLDTRKVLVFDFGSELYVWNGKNAPSDAKRAAMRLAQEHFGAENSADYADCYLNPLNYVAIVGRRENTKYAKRCAQRPEWCVLGKITQNMETVLFKEKFSDWPEIEREDLEKDYLSNGVHMVRALNGTALHKGEPYQEPNLVLEQANLGRGNFYYDTDTMRHFDVITKSTDKWQIHEFNFDAANNRDDYGHFYSAESYIVRWIYQISVTVRELSGKVSNRSTVGRDRCVYFTWQGQDSSANEKGAAALLTVELDKEKGAQMRVSQGDECTAFVRLFRQMWQHRGRKEQCLERHSEWRLYQLQGNVSEETIIKEVVCQSSHLRSRSSMLLIHGSQGNVIVWHGSKSAPHTRNVAVGVAQDLIKTVPKDLFSAETANLTEVEEGSDDDQCKKALGLKDERNEYGSLLKSTKSFDYQIRIFNFSSTQGVFKALELSDPLRCQDLHSPYPFSQSQLYNARQPTIFLLDDGDELWLWMGWWPLEDVKINTDERSSPTNENRAGVNRWISERRAALETAVDYWRAKHGENENEPFHGIKGQVVWAGLEPLAFKALFPDWTERPDVREINEEDGRTDASISIATMLAQMTQTEYPLEVLKARPLPEGVEPTRLEVYLNAEDFQAALGCSRAEFEQLPIWKQTKLKKERGLF

Target ORF information:

RefSeq Version	NM_001299990.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster supervillin, transcript variant AE (Svil), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299990.1

ORF Insert Sequence:

ATGTATCTCA TACAGACTGT TCAAACCACT TGGCGGCCAC AATTGCCGCT GCCCAGGGAG 
CTTCAGCTGA CCAATGACCG GAATAGCGCC GACGACCACC ACGATTCAAA GGTGATAACG 
ACCACCACAA CCACAGCGGA CATCCATCAC CACAACCAGC AGGAGGAGAA CGAGGAAGAG 
GATATGGATC GGCTAAGGGG CAATCACAGC ATTGGAGGAT CCTCGCAGCA GCTTTCTGGC 
AAACAGAAAT GGTCCTTCGG GCGACTTTTT CGCAAGAAAA AGGAGGCGGA GAGTGCATCC 
TCCAGCGAAG AAGATCGCAA GGCGGGATTT GTGCCTGCCC AGCGCCAGTC AGCCAGCAAG 
CCGAAGGGAA AACATGGCAA AGGATCGCGA GGTTGCAGCA AATTCGATCA CATTGTGGTG 
AGTCAGCGGC AAGAGCAGGC TCCACCACAT CCACCCCAAC CGCAAATCGA CAATGAGTTC 
TTCTTGCCCT TGGAGGCCAT ACAACCGGAG CCCTACTATC AAAATCAACC GGGCTACTAT 
CAACGATCGA GTAACTCGCT GGACAGGAGA TTACTAATGC AGCCTGGAGT TCAGGGAAAT 
GGTTACCAGC AGCCCAAATC TCGATCGGCG CAGCGAGGTC CCAAGCCACC AGGAGCTCAC 
TCCAGCGAGG AGGAACTGAT CTCGCTGAAC TCCTCCACGT TCTCCAAATA CCGCAGCGAT 
GAGAGTATTC ATTTGGGTTC CGCTGCTGGA CAAAGTCGAC GCAGTCGAGC GGCCCGCAAT 
GAGCGTTACT ACAAGAGATT GTCCCGGGAT GGCGAACCGA GTCATGGCGG AGCACCCGTT 
GTAGCCCAGC AGCGATACAA AACCCAGCCA CTGCCTTTAT CCATTTACCA GCCAACGAAC 
GCGTCCACTG GCTATGTCCA GTGGCAACCG CAGCCCCAGA AGAACCTGCA GAATGCGATG 
CAGAACGATG GAAAGCGAAG CATCAGCTAC GACAGTCAAA TACACCTGCA GAACATGAAT 
GGGAGGATGC AGAGTAAGCC ATTGCCACCG CCACCGCCTC CAAGGGATCC ACTGAGGAGA 
GTGCATGTTG GAGGTTCCGG GAAGATTGGA TCCAGCTCTG GAGACTTGCG TCCCGTGTCC 
TATGCCTTCG ATCACAATAG TCGCTGTGCG TCGGATGATC GCATTTGGCA GCCACCCCAC 
TACCAGTCGG TGCATTCGCT CAACTCCCAG CCCAGTTCGT CAATAGTCAG TGCACCAGTG 
AGTCAACAGC AACCGCACCA CAGGAGATTC ATAACTCGAT CCGAGAGGAA CGCAAATCCT 
GGCAAGCAAT CCCTGCCGAA TGGTATTGAT TTCCATTATG TGGCAGATGC AACGCCACGC 
TCCAGGAAGC CCATACACAT GATGGATGCA GGCAGGATGG ACAATACAGG AGTGCCAGCA 
TCCAGTGGTA TATTGCGAAC CTCTAAGAGT GGGCTGCCCA CACCAAGTCC TGCTCCCCAG 
CAGCCCTTGG CCAAACCGCG GTCGTTCTCC AGCTCGCGAC TTAGTGAGCT GCGAACCTAT 
CCCATGCCCA TGTACTCCGA GGTGCAGAAA CCTAAGAGGA ATGCGCCACC AGTCAATCCC 
TCGCCGAATC AGGATAACAT AGTGTGTGGG AGTCTGCACA TAAAACCCAC ACAAGAGCAT 
GGAAATCAAA ACTATGTGGA GATTCGTCAT CGGGATGTGC ACAAGACCAA CTCAATGCCG 
CACCATTATC AGAATAGGCG ATCTGGCGAG GATTTACCCA ACAAATATGA GGAGTACGTG 
GCAGAAAAGA GGGAGCAGCA TCAACAGGCA CCACCCCCAG TTTATCCGGA GCGCAAAATG 
AGCTTGACAC CAGCAGTGGC GCCACCGCCT CCTGTTTACT TCCCTCGCAA GAAGCCTGCC 
AATTTGGAGG AGGCCATCAA CGAACTGGAG GCCATTTACA AGTCTCTAGG ACTCACTGAA 
CCAGCCGAGC CCAAACAGGA ACCAAAGCTG GATATTTCCT CCAAGATGCG TGTGCCAACT 
CCCAGTGATT TTGAAAAGTT CGCCTTGGCG CATGCTGATG ACTACGAGGA TGAAGATTCG 
CCAACCGGAG AACCAGATCC AATCAGAGAT GATGTGGCCT TCAGGAACCT ACAGTTGGCC 
AATCTGCAAC ATCGGCCATC GGAGAAGCAG CCTCCATTCG GCATCCCAGT GGGACCCATT 
GTGCCAGCTC CACAGTCGGA TTATCTTCAC GTAGAGCCAG TGAGGGTGAA GAAGAAGAGT 
GGCTCTCCTG ACATCATTAA AGATGATCTT GCTGTGCGTT CATTGAGAAA GGATCCTCCT 
GGTCCCAAAT CCAGCTTTGT CTACCCAATG CAAAAGAAGC AACGGGCTAT TCGAACCCAA 
TCGGCCAACA TATACAATCT GATCCAAAGA GACGCTGCCA AGCCATCTGG TGGAGATCTT 
AAGAGCTACA TGGAGCTGAC TCGCAACCTG GAAAGAGCTG GCAGCATGTC CAATCTGCAG 
GGTGAGGATG GCAGTGATAC CAAAGATGTA CCCGCCACAC TGGATCTTCT CCGAAAGTTG 
AAAGCTCAGG ATGAGGAACT GGATTTGGCT AGAAAGCATC ACCCCATACC GTTTAGACAT 
CCCAGTCAGG GAGGAGCTAT TGCCCAACTG CCAGAACGGT TGTCCAAAGA GGAAATACCC 
AAGGCAGCGC CTGTGCCAGC TCCCCGAAAG AGTCTGACTC CTGAACCCAT GCTAGATGAT 
GCTCTCAACA AGATTGCCCA GGATGCGCAG GCCAGCAGCA TTAAGCTGAG TCAGGAACTC 
CATGAACTTC GCAAGGAGGC ATTGATTACA GCTGCGAGAC CAAAGTTGAA TGCCGAACAA 
CAGAGATTAG AGGATGAACT GCAGCAGATA GAAGCTGTCT CCGAGGCAGC CAAACGTTGT 
GGCCAAATGC TGCTCGAAAC TCTGCCAGAT GCGGGACAGG AGGGTCAGGA ACCTCAGGAT 
AAGCCCCTCA GGAAGCTACA TAAGGAGGGC AAACTCATAC GAGCTATCGA TGAGGTGTCC 
GAAGCTGCTA ATGCGGTCTG TGAGAAGATC CTCAAGGACA TTGTGACCAC AGAACCACCG 
GTGGTAGTTC AGGAGGCCCT CCAAGTGAGG CAGACCACAA AGACTGGACC AGGCGGAGAA 
CAGCTGGTGA TGCCGCATCT TATCAAGAAA CTAGATCCCA TACAATCCGA CAAGATCGAG 
CACATTGCCA GACGATGCAT GCGGCAGTTG AGCGAACTGG CCAATAATCC GGACTACGAC 
AACCTGGCCA CCTGTAATAT GAGCAACAGC ACCACCAACG CAACGGGTGG AGCAGCCGCT 
GATGCCACTC TCGTTTGTCC CGTTCCAGAG ACCCAAGCCA AGAGCCAGAC GCTCGAGGAG 
ATTGACAAGA TCATGCAGGA GTGCGAGCGT CAGGCGAAGA GCAGCAGCTC AACCACGACC 
ACCGATGATC AGCGCACCAC TGCCCCCAGT CTGACGAGTG GAAGTGGCAG CGGGAGCTTC 
CCACCCAATG TGAGCACCAC CGCCAGTTCG TTCAGCTCAA GCAGCGACTG TTTGGCCAAG 
TCGTCGAGTC CTTCGCGCGC CAGCCGTCCG CTCTCCTCGA GCATCACCTC TTTCAATCCA 
TATTCCTCCA GCGACTACAT CAAGTCGCAG AGCAGTGATT GCCATGCACC AAGCACCGAT 
CCGATCAAGA CCTTTAGCAC CACCTCGTAC GAGGTGCAGT CCACACCGCA GAGCACCACC 
ATTAGCACCA ATACGATGAG CACGAGCCAG AGCAACACGA TCAGTAGCAC TACTAATCCG 
AGTGTGGAGT CCAAGCCATC CGCCCCGTCG GTATCGCCAC CACCCCTCCA GTTGACGCCA 
GTGGGTGATC GGAGGGGCAG GAACCGGGAG CGGGGAGCTT CCTGCTCCTC CACATCCTCA 
CCTTCACAGT ACAACTCGAG CGAGGAGCTG GCCGCCATTT TTGGCATCGA GGAGCAACCG 
AAACAGCAGC GTCGCAGTCG GCAGACGCAT AATGCCACAG AAACCATTGC GGCCTCAAGT 
GACTTATCCA CAGCACCTGC CACTGCCAAG ACCAGCTGCC AGCCACCTGA GCCACTGAAA 
CAGAAGCCAC CGCAGCAGCA GACCACCAAG GACTCGGAGG ATTCCAGTTC ACAGAAGAGT 
GCTCCAGTCC AGTCGTATCG TGGTCAGCAT CCGCACTTCC ACCATCACAC CACGCCCGCT 
TACTACACGG ATCTTCAGCT GAGAATTCAC ACGCCGAACG CATCGGATGT GGGCGCCTTG 
AACGAGAACT ATCGAAGTGT ATACAGTACT CCGAGCACCC AGCTGGTGGA ACAGCGATCG 
TTTCCAAAGA GCTCCACGGC CACGAAGGAT CCACCGCTGC CCATAAGCAA TCCGGCTAGG 
GGAACATTTT TTCGTGGCGA CTCTGCGTCC AGCGACAGTC ACTCAAGACT CCGTCGGAGC 
CAGCAGAGAA CCGGCAGCGA GGGACGCTTC TTCAGATACT CTCAGGGATC TGTGAGCCCT 
ACCAAAGGTG AACCTCCAAT CGGCCACGAA GGTGGTGAGC AGCCACCCGT TGTCGCCAGT 
CACTCCCCCA TCACATCCAC CAGCACCACC ACCATCAGTA GTTGTAACCC CGTAGAGCAG 
GCTAGTCCAC CACCAACAAA CGTAGTAAAT CCCAGCGGAT CCGGTCGCAA CAGCCGGCAC 
AAGCAGCGTT TCCACAGTCG ATCCCGTCGA GCGGTGCGCG TGCGCAGTGA ATCGCGTCCG 
ATTAGCGCCC TCTATGACAT AATATGCAAG GAGAAGAACC TGGAAAACAG CAGCAGCAGC 
AGCAGCAGCA GCAACGAGCA GGAGAAGAAA AAGGATCAGG AGCAGCCTAA GACCACAACC 
GACAAGCTGG CGACCTTTTG GCCCCTGCAC CATCACTACC TCAATCCCCC GATGAGCAGC 
GGCAACTCGG GCGGCAGCAG TGGCAATCCC AAGCAGCAGC AGCAGCAGCA GCAGCAACAA 
CAGCTAGAGC AGCTGGGACA CCAGACACAT CTTGTGCTAG GAGCATCAAA GGAGTTGCCC 
GCGGTCAGCA GCAGCAGCGA TGAAGAGGGA TCCCTGCGGA CTCTCCACTC CAGTCGCAAG 
GTGACCAATC TGAATGCCAA GTCCACCTCT CTGCCACCCG AGGAAGGACT CTCGGGTGAT 
GTCTCCCTGG AGTCGTCCTC CAACCGACTG AATCTGCGAT TGGCCAGGAG CCTGGAGCCC 
TCAGTCCGCA ATATCACTGC ATCCGCCTCC AAGGAAGTAC AGAGCTATGA TGCTGGGGAG 
CCTGTGAATA CCAATCGATT GTTTGTGGAA CCGCCCAAGA TACCACTGTC TGCCATCCGA 
AACTCTCTTG ACGATCACGA CCTGGATATT GAGGAGTGCG ACAGCACCAC CCAGTTCGAT 
CAGGGGGATA TCTACGATAC CCTGGAACGC TGGAGCCATG GTGTCAGCTT GGGGGCACAT 
CTATTGAACT GCGCGAATGG CCACGATCAA TCTGATCCCG AGGACGACCA TCAAACCGAT 
CATCATCAGC ATGCACATCA ATCATTGCCA GTAGAACCCA AGTCTCATCA TCATCAGCAC 
CACCAGGCCA CCAATGCGCG CAGATTCATA ACGCGCACCA CGCGTTCCGC CAGTCGCCAG 
AGTTCCGCCG GACACTCCCC ACCTCCGCCG CTGCATCGCA GTGACGACTC CTCCAGCAGT 
TCACCCTCAC CGACACGCAG ACGGAATAAG CCAGGTGGTG CTCCTGCCGC CACAGATCCA 
CCATCATCGT CGGCGAACGG GAATAGTTCC GCTTATGAGA CGGCAGCCAC CACTGTGATG 
ACCCATGACC AGCAGCGAGA TCCTCCAGTT GAAGTTCCTA AGGCTCTAAA CGTCGAGCCA 
CCGCAGCCAG AAGACATCGT CCTGGGCTCC TGCCCCACTG CATCTGCCCA CTCAGCAGAG 
AGCCAAAGTC GAGGAAAAGC TGGCAAATGC AGTATTAATT GCACCTACAG CCACAGCCAC 
AGCACCAACA ACAACTGCAA CGCGCGACAA CGCAACAGCA GCAACATCAG CAGCAACAAC 
ACCATCAGCA GCAACAGCAG CAGCAGCAAA AGCACTTGCA ACAACAATAG CAAACAGCAA 
CACGCCACGC CCATGCCGCC CCTAGCTAAT GCCAACAAAG CCCCGTTAAT TGTGCTTAAG 
ACAGCCGCAT CTGCAGCAGC ATTGCCGTCC ATTGCCGCAG CAGCAACATC CAGCGGCACG 
GCAGCAACAT CTGGTGGTGT TGGTTCGCCC ATCAAGCGTC GCAAGAATCC GCTGTCGCCG 
CTGCTCCTCG GCAGCCAGTG CCAGTCCACC ATTGCGCCTC CGCAGGCCAT TGCCAGTCCG 
CTCCTGAGCG GCGGCAGCTG CTCCTCCTCG ACCACCTCGC CCCAATCCTC GCCAGCGGTT 
CGCACCACCA AGGCGAGTCG TCTGCGCGCC GCCGCGCTTG ATAAAAAGAA GGAAGAGGAG 
CAACGCAATC GCTACAGTTC GCCCGTTTCA CCAAAAGCCT CCAGCAACGG CGGAGTCCAA 
GCACAACGTC GTCTTAGCAA CTCGGAGCTA TCGCCCAAGT CGCCAGGTGC CAGTGGATTC 
ACTTCGGAGC CAGTCGCGTC AGCCACAGCG AACAGAGTCC CGACGAGATC TGCCTCCCAT 
CCTGTGGACA CACCGCCCAC ACAAAGGCGA CAGCCTCTGG TGGCCGTGAG TTCGGGCATC 
TCCATGAAGC AGAGTCTCAG CGAGCTGCAC TTCACGGGTG GAGCAGTCAG TCCGCCCAAG 
GCTACGGCGG AATGCACTCT AAGGATGCGA CCACGTAGCT CCACGATGAG CACCGAGAAC 
ACGGAGAATG GCCGACGTGA CCACTTGGCT AATCTGCAAC TAAGTCCTGT GCAAAAGCAA 
AAGCGAACGC CCGAAGTATC ACCACGCCGT TTCGGAGATC GCGACAAGTC GGCCAAACGG 
AAATCGAATT TGAATCGTTC GTTGACCGAG GAAGCCACCA AGGATGGCGA TGAGGTTGCC 
TCTTCGACCC GCCGGCGCCG ACGTCGTGAA CTGGGAATGG CTCGTCCCCT GGTTCCGGGC 
GATGATGACG CCCTAAGAGA TTTGCTAACG CCGCAGAAGA GTTCCTGCAG CGAGTCAACC 
AACTCCATAT CCGAACCCCA TCTCCAGTTC ATACCAGCCG TTCGTCATCC GGTGGTTCCA 
GTACGCACCA TGACTGCTGT GCTGCCTGCA CCACGACCGC TGCATACTCC TGCGGCCCGC 
AACGTCAGCC AGAAGTTCAA TGAAAGGACC AAAACAGATC TGGCCGAGAT CAAGAAGATT 
GCTGAGCAAT CGGAGGCGCA GGTGCAACAG CCTTCGGTGG ATGATAACCA GAAAATGTCA 
GCCATAATGC GCAGGAAGAG CACGGATGAG GGTGGATCAG CTTTGGGGGC AACAAACCAG 
AATTCACCAC CTGCCCCAAA CCAAATTGTA TCCATACTGA AGAAGAAGGA ACATGGACTT 
GGCGAGTGCA ACTCCAGTGC TTCAAGCAAT CCCTCGCCGG TGACCTTCTC TTCTAGCGTG 
GTGGATCATC CTTTGGCTGG AGCTGGCCGG TGCAAACGGC AGGGAATACT CAAGAAGAGA 
TCGAGTCTAG ATGAATCGCG CTACTACTCC CGATCGCATT CACCCGACGA ACGTAGTATT 
CTCATTAAGT CGGCCAGGAG GAATTCCCTG GAGGAAGCTG GCACTGGATT GAGTCCGGCT 
CAGGCCCATG GAATCCTCAA GCAGAGCAGC TACGACAGCA GCAAGAGCGA CGGCTGCCCT 
TCGGCCACGG AGAGCCAGCC ACACAGTATC CTGAAAAAGA AGGATTCGCT ATCGACACCT 
TCGGACGGAG GATGCCACAA GCATGTCTCT ATATCGCAAG CTGTAACTTT AGCTGCTGCT 
GAACTGGCTG CCCATGATGG AATCACCTTC GATGATGGTG AGGAGCATGA GATACGACCG 
ATCCTAAAGC AGGAGAGCAC CTCCAGCGAG GAGGCTGTGC GTCCGCCAAA GCCCATACTC 
AAAAAGAAAT CCTTTGGCGA GGCGGATGAA CACGAAATAC GTCCTATATT GAAGAGCTCT 
CGTAAAAGCA GTCGAGAGGA GTTCGATTTG AGTGGCTTGG AAAACGAGGT CAACGATTCC 
CTATCGTCAA TCCTAAAGAC AGACTCGCCG TCCAAGCGTC GGTCGCTCGG TTCAGCTTTG 
CATGATTTGG AGGAGTCCAC CACTTCCCCG AGCATGCTCA AGAGGCGCAC CCGTTCGCTG 
GAACGTCAAG ATGTCCAGCC TGGCATGGAT TTGGCAGCTG CACTGGATGC CATCGCCACA 
TCACAGGCTA CTCCCAGCAC CCCGGTGGAC TTCGTGACCA CACCAACAAG TATATCGGTG 
GCGGAGCGGA TCAAGCGCAT GGAGATGCTG TCGACGCCCA CTTCCCGTGC GGCGGGTGGG 
GCCAACAGCA GCTGGGAATC GCCACTGCAG GCATCGTCAG CAGAGCATGG AGCACCCACC 
ACCCCAAGGG TTCTCAAGCC AAGTGTGCTG CGCAGAGATT TGCAGAGGGA GCGGTACAAA 
ACGCAACCCG TGACCAACGA GGAGATGAGC TTCTTCAAAG CCAACTCCGC GCCCTTCGAC 
ATCTCGGCTA CATCGGCCTT CAAGCCCACC AAGCCGCTGG ACATCCGCTT GACCCACGCA 
CCGCATGCAC CGCTGATTTC GCCGATCACC GGACAGCCAC TCAGCCACGT GCCCGCGCCA 
CTGCTGCTAT CCTCGTCGAC GAACTCGGGA GCGGGATCCT CGTTCCGTCT GGCCCGCAGC 
TCCACCCAAC CGCTGCAGTC GCCGCGAGTT GTCCACCTGG CCGGTCAGGC TACTGGTGGC 
TCCGCTTTGG CACGCCAGAA CTCGGTGAAC GACAGCATCA ATCTCAGCGA GCAGCTAACC 
CTATCCATCG GCACGGACAG CGAGCACATC TTTGAGATGT CCGCTTTGGA GGAGGACTCG 
GCTATTCAGT CGCTGAGCGA CGAGACAGCC GCTTCCGCAT CTGCCAACTC AACATCCACG 
ACCACTGCAA CCACCATGAC ACCGCCCAGT GGCCTGACGC GGAGCAACAG CGTTCGGGCC 
AGGGCCAATA TGTTCCAGCA ATTGCAGGAG CAGTCGCGCA ACCAACGGGC AACGGGAACC 
ACCAACTCCC GTCAATCTCC ACCAGCGTCG TCGGCGGAGG TGTCTTCAAG TGCAGCCGAC 
AATTCCATGC ACAGTGACGA GCGGTCCACG CCAAATGCAA CGATAGATGC GGAATTCGGA 
AAACCCAGCA CCGAACCACT TAAGAGCATT CTTAAGACGG GAATGCCAGT GATGGGTATG 
GGACGCGGTT TAATGCCTGT GGATCTCAAT GCCGAGCTGA AGAACCGGCT CAAACGCTCC 
ACACATGCCA CTGTTTCCAA TCTCCGCAAA TCTGCCACCT CGGCAGATGC CACTAGAGCA 
GCTAGTGATG AGGTGGACAA TCCGCAGAGG AATTTAGCCC AGATCCTGAG GAATGTTTCC 
AAGGAGAACT CCACGCCGAA GCACGATGAT GCCGTGAGCC TAGTGCGGAA TCTCTCTACT 
TTGGGACAAC CTCGATCGCT GGCCCGTGAC GATGCAGCCG GGAATTGTGC CTCGGCATCC 
GAAGGCGAGA GTTCGGGGGG CCGCGAGATC GAGGCCATAA TCAAGAACAG CGCTGTGGCC 
AGACGTCGCC GGCAGCAACA GCAGCAGTCG CAGCAGCAGA ATCAGCCAGA TGGCAATCTC 
GTTGCGAAAA GCAAAAGTCA CTCGGGCATA TCAGCACCCA TCGGCCTTGG CCCCCAGCTA 
CCACCCCTTC CCCTCGGCGG CGGTTTTGTT AAGTTGCGTC ATGTAGCCAA GGAGGAGGAT 
ACCAGTAGCA GCAGCAACAC ATCCTCCGCC AAGGAATCGC CAGATAAGAA GGATCTTCGC 
CAGGAGCAGG CAGAAGCTTC GGGAGACTCA TCGCACCCAA CGGCTCTGCC ACAGTTTCTT 
CAAGGCGTCC AACGATCATC AACCGAGGTC ATGGGCCCCG ATCAGCGGTC TTTGGTGATT 
TCGAAGACCG GCTCCATTGC GGAGCGTTTG GCGGCACTCC ACAAAAGTGG CGAGGACGAC 
TGGAAGAAGC GCATCTCGAA GCGCGACGAA GTCGACGATG TGCACCGTGA AAACTTCGTA 
AACGAATCGC TATCTTTGGC CCACTCGCTG TCGGACAAAC CACTGCCCCC ATCACCTTTG 
ATACCCACCA GCTTGGAGGG CGGCAAGGTC TCCGATCGTT TGAGCAAACT CAAGTGCAAC 
TCGGAGAACT GGAAGCAGCG CATAGAGCAA ACCGATGCCA AGAAATTCAC AGTCGCCGGT 
CGACTGCAAA AGAAGGCCCA ATCCCCCGTG GAGCTTCAAT TCGAGCGTTC TGCCAATGAT 
GCGGCCAAAA AGTGTCCCAT GCTGGAGGTG CGCAGCGCCA ATCAGCCGCA GCTTGGGCTG 
GCCAAGAGCC CCTCCATGAT GGTCACCTCT AGCACCAGCA CCAATAGCAC CAGCAAGTCC 
GCACTCCGCA GCCTGAGCGT CAATCCCGAG GAGGAGGGAC CCACCAGCCT GAGTCGCTCC 
AACAGCAGCA GTTCGGAGTC CGATGGAGAG CGCAAATCCC CCCAGAATGC CAAGCAGAAC 
AAGGAGCTGG CCAACAACAA GAGTGCTGCA GCACCCACCA ATGTGGGAGC CAGGATTCAG 
GTGCCCCGGC TGGACGACAA GGAAACTTTC GAGAACTTCT TCTCCTCCAA GCCGAAGAAG 
GAGGAGAACG TTCAGCTGGA GATCAGCGCT TTCGATGACA TCAAGCCCAC TGAGCGCTTG 
GTAAGCAAGC GACACGTACA GGGTCCCAAA GGTCGACGGG CTGCACGCAA TCCTCTCAAG 
AGTTTAGCTG CTCGTGAGGA CATTAGCTCC GAGTACACCG AAATGAGGTC GGGCATAGCC 
GAGCGAGAAT TACGGCGCCT AAAGTTGGAG TCATATGGTC ATAATGCCAA CTTGGCTGCA 
GAGGCCATAG CCGGATTAGC CTCCGTCGAG GACTTCAAGT CGGTGGCACT GCGCTCCTCG 
TCGATTCCAC TGCAGCAAAT GTGGCTACCC CACAAGCCAG TGATGTTACT CCATGTTAAG 
GGGCGCACAC ATGTCCAAAC CCGACTGGTG GAACCCGTGC ATACCTCCCT GAACCGTGGT 
GATTGCTTCA TCCTGGTGGC CGGATCGCAG CTTTTCCGCT ACGTTGGATC CTTTGCAAAC 
GTAATCGAGA TCTCCCGGAG CAAGAAGATC TGCGCTGCCA TAGTGGAAAA CAAGGATCTC 
GGTTGCACCG CCACCCAGGA GGTGATCCTC ACGGATGGCA AGTATTTGAA GGAGCGCCAG 
TGGCGTCAGT TCTGGCAGCT TTTGGGCAAA AAAGAGGATG ATCAATCGGA TATAGCTGAA 
TGCGGTCATG CCGATGAAGA CGACGTGTTC GAGAGCAGCC TGATCGAGAC TAACAAGATC 
TACGAGTACC AGGATGAGGC GCTCATTCCG TTGGAGAAAT TCTGGGGCTG CATCCCCAAG 
GTGGACATGT TGGACACGCG TAAAGTTTTG GTCTTTGACT TTGGCAGCGA GCTGTATGTG 
TGGAATGGCA AGAATGCCCC TTCCGATGCC AAGCGAGCTG CCATGAGATT GGCCCAGGAA 
CACTTTGGTG CTGAGAATTC GGCGGACTAT GCCGACTGTT ACTTGAATCC CCTGAACTAT 
GTCGCCATAG TGGGTCGTCG TGAGAACACC AAGTATGCCA AGAGGTGCGC ACAGCGTCCA 
GAATGGTGTG TTCTCGGCAA GATCACCCAG AACATGGAGA CGGTGCTGTT CAAGGAGAAG 
TTCAGTGATT GGCCAGAGAT AGAACGCGAG GATCTGGAGA AGGATTACCT ATCAAACGGA 
GTGCATATGG TGCGTGCTTT AAACGGCACC GCTTTGCATA AGGGAGAACC CTACCAGGAA 
CCAAATCTGG TGCTCGAGCA GGCCAACTTG GGGCGTGGCA ATTTCTACTA CGACACGGAC 
ACCATGCGGC ACTTCGATGT GATTACCAAG TCAACGGACA AATGGCAGAT CCACGAGTTC 
AACTTTGATG CGGCGAATAA TCGCGATGAC TACGGTCACT TCTACTCGGC GGAGAGCTAT 
ATAGTGCGCT GGATCTACCA GATTTCGGTC ACAGTTCGCG AACTCAGTGG CAAGGTGTCG 
AACAGAAGCA CAGTGGGTCG AGACAGGTGT GTCTATTTCA CCTGGCAAGG ACAGGACTCA 
AGTGCCAACG AGAAGGGTGC AGCTGCTTTG CTGACCGTAG AGTTGGACAA GGAGAAAGGC 
GCACAGATGC GGGTGTCGCA AGGAGATGAG TGCACAGCCT TCGTCCGCCT GTTCCGGCAA 
ATGTGGCAAC ATCGTGGACG CAAGGAGCAA TGTCTGGAGA GGCACAGCGA GTGGAGGCTC 
TACCAACTGC AGGGCAACGT CAGCGAGGAG ACCATCATCA AGGAAGTCGT CTGCCAGTCC 
AGCCATTTGC GATCCCGCAG CTCCATGCTG TTAATTCACG GCAGTCAGGG AAATGTGATT 
GTCTGGCATG GATCGAAGAG TGCTCCTCAT ACGCGTAATG TGGCTGTAGG AGTTGCTCAG 
GATCTGATCA AGACGGTGCC TAAAGATCTC TTCAGCGCCG AAACTGCCAA CCTAACTGAG 
GTGGAGGAGG GTTCCGACGA CGATCAGTGC AAGAAGGCCC TGGGATTGAA GGATGAAAGA 
AATGAATATG GCAGCCTGCT TAAGTCCACC AAGTCGTTCG ATTACCAAAT AAGGATCTTC 
AACTTCTCCA GCACTCAGGG TGTTTTCAAA GCCCTCGAAC TGAGCGATCC GCTGCGCTGC 
CAGGATCTGC ATAGTCCGTA TCCCTTCAGC CAGTCGCAGC TGTACAATGC AAGGCAGCCG 
ACTATCTTTT TGCTGGACGA CGGCGATGAG TTGTGGCTCT GGATGGGATG GTGGCCTCTG 
GAAGATGTGA AGATAAACAC CGATGAGCGA AGCAGTCCCA CCAACGAAAA TCGCGCTGGA 
GTAAATCGCT GGATCTCAGA GAGACGTGCT GCCCTCGAGA CAGCCGTTGA CTATTGGCGT 
GCCAAGCACG GCGAGAACGA GAATGAACCC TTCCATGGCA TCAAAGGCCA GGTTGTGTGG 
GCAGGCCTGG AGCCACTGGC CTTTAAGGCG CTGTTTCCCG ACTGGACAGA ACGTCCGGAT 
GTAAGAGAAA TCAACGAAGA AGATGGGCGA ACTGATGCTT CCATATCGAT CGCCACAATG 
CTGGCACAAA TGACGCAGAC CGAGTATCCC CTGGAAGTCC TGAAAGCCCG ACCGCTGCCC 
GAAGGTGTGG AGCCCACCCG CCTGGAGGTC TACCTTAATG CGGAGGACTT CCAGGCGGCA 
CTGGGATGCA GCCGGGCGGA ATTCGAGCAG CTGCCCATCT GGAAACAGAC CAAGCTGAAG 
AAGGAGCGAG GGCTGTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Svil ( NM_001299987.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286916.1 Drosophila melanogaster supervillin, transcript variant AB (Svil), mRNA.

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CloneID	OFa21975
Clone ID Related Accession (Same CDS sequence)	NM_001299987.1
Accession Version	NM_001299987.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	supervillin, isoform AB
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTATCTCA TACAGACTGT TCAAACCACT TGGCGGCCAC AATTGCCGCT GCCCAGGGAG 
CTTCAGCTGA CCAATGACCG GAATAGCGCC GACGACCACC ACGATTCAAA GGTGATAACG 
ACCACCACAA CCACAGCGGA CATCCATCAC CACAACCAGC AGGAGGAGAA CGAGGAAGAG 
GATATGGATC GGCTAAGGGG CAATCACAGC ATTGGAGGAT CCTCGCAGCA GCTTTCTGGC 
AAACAGAAAT GGTCCTTCGG GCGACTTTTT CGCAAGAAAA AGGAGGCGGA GAGTGCATCC 
TCCAGCGAAG AAGATCGCAA GGCGGGATTT GTGCCTGCCC AGCGCCAGTC AGCCAGCAAG 
CCGAAGGGAA AACATGGCAA AGGATCGCGA GGTTGCAGCA AATTCGATCA CATTGTGGTG 
AGTCAGCGGC AAGAGCAGGC TCCACCACAT CCACCCCAAC CGCAAATCGA CAATGAGTTC 
TTCTTGCCCT TGGAGGCCAT ACAACCGGAG CCCTACTATC AAAATCAACC GGGCTACTAT 
CAACGATCGA GTAACTCGCT GGACAGGAGA TTACTAATGC AGCCTGGAGT TCAGGGAAAT 
GGTTACCAGC AGCCCAAATC TCGATCGGCG CAGCGAGGTC CCAAGCCACC AGGAGCTCAC 
TCCAGCGAGG AGGAACTGAT CTCGCTGAAC TCCTCCACGT TCTCCAAATA CCGCAGCGAT 
GAGAGTATTC ATTTGGGTTC CGCTGCTGGA CAAAGTCGAC GCAGTCGAGC GGCCCGCAAT 
GAGCGTTACT ACAAGAGATT GTCCCGGGAT GGCGAACCGA GTCATGGCGG AGCACCCGTT 
GTAGCCCAGC AGCGATACAA AACCCAGCCA CTGCCTTTAT CCATTTACCA GCCAACGAAC 
GCGTCCACTG GCTATGTCCA GTGGCAACCG CAGCCCCAGA AGAACCTGCA GAATGCGATG 
CAGAACGATG GAAAGCGAAG CATCAGCTAC GACAGTCAAA TACACCTGCA GAACATGAAT 
GGGAGGATGC AGAGTAAGCC ATTGCCACCG CCACCGCCTC CAAGGGATCC ACTGAGGAGA 
GTGCATGTTG GAGGTTCCGG GAAGATTGGA TCCAGCTCTG GAGACTTGCG TCCCGTGTCC 
TATGCCTTCG ATCACAATAG TCGCTGTGCG TCGGATGATC GCATTTGGCA GCCACCCCAC 
TACCAGTCGG TGCATTCGCT CAACTCCCAG CCCAGTTCGT CAATAGTCAG TGCACCAGTG 
AGTCAACAGC AACCGCACCA CAGGAGATTC ATAACTCGAT CCGAGAGGAA CGCAAATCCT 
GGCAAGCAAT CCCTGCCGAA TGGTATTGAT TTCCATTATG TGGCAGATGC AACGCCACGC 
TCCAGGAAGC CCATACACAT GATGGATGCA GGCAGGATGG ACAATACAGG AGTGCCAGCA 
TCCAGTGGTA TATTGCGAAC CTCTAAGAGT GGGCTGCCCA CACCAAGTCC TGCTCCCCAG 
CAGCCCTTGG CCAAACCGCG GTCGTTCTCC AGCTCGCGAC TTAGTGAGCT GCGAACCTAT 
CCCATGCCCA TGTACTCCGA GGTGCAGAAA CCTAAGAGGA ATGCGCCACC AGTCAATCCC 
TCGCCGAATC AGGATAACAT AGTGTGTGGG AGTCTGCACA TAAAACCCAC ACAAGAGCAT 
GGAAATCAAA ACTATGTGGA GATTCGTCAT CGGGATGTGC ACAAGACCAA CTCAATGCCG 
CACCATTATC AGAATAGGCG ATCTGGCGAG GATTTACCCA ACAAATATGA GGAGTACGTG 
GCAGAAAAGA GGGAGCAGCA TCAACAGGCA CCACCCCCAG TTTATCCGGA GCGCAAAATG 
AGCTTGACAC CAGCAGTGGC GCCACCGCCT CCTGTTTACT TCCCTCGCAA GAAGCCTGCC 
AATTTGGAGG AGGCCATCAA CGAACTGGAG GCCATTTACA AGTCTCTAGG ACTCACTGAA 
CCAGCCGAGC CCAAACAGGA ACCAAAGCTG GATATTTCCT CCAAGATGCG TGTGCCAACT 
CCCAGTGATT TTGAAAAGTT CGCCTTGGCG CATGCTGATG ACTACGAGGA TGAAGATTCG 
CCAACCGGAG AACCAGATCC AATCAGAGAT GATGTGGCCT TCAGGAACCT ACAGTTGGCC 
AATCTGCAAC ATCGGCCATC GGAGAAGCAG CCTCCATTCG GCATCCCAGT GGGACCCATT 
GTGCCAGCTC CACAGTCGGA TTATCTTCAC GTAGAGCCAG TGAGGGTGAA GAAGAAGAGT 
GGCTCTCCTG ACATCATTAA AGATGATCTT GCTGTGCGTT CATTGAGAAA GGATCCTCCT 
GGTCCCAAAT CCAGCTTTGT CTACCCAATG CAAAAGAAGC AACGGGCTAT TCGAACCCAA 
TCGGCCAACA TATACAATCT GATCCAAAGA GACGCTGCCA AGCCATCTGG TGGAGATCTT 
AAGAGCTACA TGGAGCTGAC TCGCAACCTG GAAAGAGCTG GCAGCATGTC CAATCTGCAG 
GGTGAGGATG GCAGTGATAC CAAAGATGTA CCCGCCACAC TGGATCTTCT CCGAAAGTTG 
AAAGCTCAGG ATGAGGAACT GGATTTGGCT AGAAAGCATC ACCCCATACC GTTTAGACAT 
CCCAGTCAGG GAGGAGCTAT TGCCCAACTG CCAGAACGGT TGTCCAAAGA GGAAATACCC 
AAGGCAGCGC CTGTGCCAGC TCCCCGAAAG AGTCTGACTC CTGAACCCAT GCTAGATGAT 
GCTCTCAACA AGATTGCCCA GGATGCGCAG GCCAGCAGCA TTAAGCTGAG TCAGGAACTC 
CATGAACTTC GCAAGGAGGC ATTGATTACA GCTGCGAGAC CAAAGTTGAA TGCCGAACAA 
CAGAGATTAG AGGATGAACT GCAGCAGATA GAAGCTGTCT CCGAGGCAGC CAAACGTTGT 
GGCCAAATGC TGCTCGAAAC TCTGCCAGAT GCGGGACAGG AGGGTCAGGA ACCTCAGGAT 
AAGCCCCTCA GGAAGCTACA TAAGGAGGGC AAACTCATAC GAGCTATCGA TGAGGTGTCC 
GAAGCTGCTA ATGCGGTCTG TGAGAAGATC CTCAAGGACA TTGTGACCAC AGAACCACCG 
GTGGTAGTTC AGGAGGCCCT CCAAGTGAGG CAGACCACAA AGACTGGACC AGGCGGAGAA 
CAGCTGGTGA TGCCGCATCT TATCAAGAAA CTAGATCCCA TACAATCCGA CAAGATCGAG 
CACATTGCCA GACGATGCAT GCGGCAGTTG AGCGAACTGG CCAATAATCC GGACTACGAC 
AACCTGGCCA CCTGTAATAT GAGCAACAGC ACCACCAACG CAACGGGTGG AGCAGCCGCT 
GATGCCACTC TCGTTTGTCC CGTTCCAGAG ACCCAAGCCA AGAGCCAGAC GCTCGAGGAG 
ATTGACAAGA TCATGCAGGA GTGCGAGCGT CAGGCGAAGA GCAGCAGCTC AACCACGACC 
ACCGATGATC AGCGCACCAC TGCCCCCAGT CTGACGAGTG GAAGTGGCAG CGGGAGCTTC 
CCACCCAATG TGAGCACCAC CGCCAGTTCG TTCAGCTCAA GCAGCGACTG TTTGGCCAAG 
TCGTCGAGTC CTTCGCGCGC CAGCCGTCCG CTCTCCTCGA GCATCACCTC TTTCAATCCA 
TATTCCTCCA GCGACTACAT CAAGTCGCAG AGCAGTGATT GCCATGCACC AAGCACCGAT 
CCGATCAAGA CCTTTAGCAC CACCTCGTAC GAGGTGCAGT CCACACCGCA GAGCACCACC 
ATTAGCACCA ATACGATGAG CACGAGCCAG AGCAACACGA TCAGTAGCAC TACTAATCCG 
AGTGTGGAGT CCAAGCCATC CGCCCCGTCG GTATCGCCAC CACCCCTCCA GTTGACGCCA 
GTGGGTGATC GGAGGGGCAG GAACCGGGAG CGGGGAGCTT CCTGCTCCTC CACATCCTCA 
CCTTCACAGT ACAACTCGAG CGAGGAGCTG GCCGCCATTT TTGGCATCGA GGAGCAACCG 
AAACAGCAGC GTCGCAGTCG GCAGACGCAT AATGCCACAG AAACCATTGC GGCCTCAAGT 
GACTTATCCA CAGCACCTGC CACTGCCAAG ACCAGCTGCC AGCCACCTGA GCCACTGAAA 
CAGAAGCCAC CGCAGCAGCA GACCACCAAG GACTCGGAGG ATTCCAGTTC ACAGAAGAGT 
GCTCCAGTCC AGTCGTATCG TGGTCAGCAT CCGCACTTCC ACCATCACAC CACGCCCGCT 
TACTACACGG ATCTTCAGCT GAGAATTCAC ACGCCGAACG CATCGGATGT GGGCGCCTTG 
AACGAGAACT ATCGAAGTGT ATACAGTACT CCGAGCACCC AGCTGGTGGA ACAGCGATCG 
TTTCCAAAGA GCTCCACGGC CACGAAGGAT CCACCGCTGC CCATAAGCAA TCCGGCTAGG 
GGAACATTTT TTCGTGGCGA CTCTGCGTCC AGCGACAGTC ACTCAAGACT CCGTCGGAGC 
CAGCAGAGAA CCGGCAGCGA GGGACGCTTC TTCAGATACT CTCAGGGATC TGTGAGCCCT 
ACCAAAGGTG AACCTCCAAT CGGCCACGAA GGTGGTGAGC AGCCACCCGT TGTCGCCAGT 
CACTCCCCCA TCACATCCAC CAGCACCACC ACCATCAGTA GTTGTAACCC CGTAGAGCAG 
GCTAGTCCAC CACCAACAAA CGTAGTAAAT CCCAGCGGAT CCGGTCGCAA CAGCCGGCAC 
AAGCAGCGTT TCCACAGTCG ATCCCGTCGA GCGGTGCGCG TGCGCAGTGA ATCGCGTCCG 
ATTAGCGCCC TCTATGACAT AATATGCAAG GAGAAGAACC TGGAAAACAG CAGCAGCAGC 
AGCAGCAGCA GCAACGAGCA GGAGAAGAAA AAGGATCAGG AGCAGCCTAA GACCACAACC 
GACAAGCTGG CGACCTTTTG GCCCCTGCAC CATCACTACC TCAATCCCCC GATGAGCAGC 
GGCAACTCGG GCGGCAGCAG TGGCAATCCC AAGCAGCAGC AGCAGCAGCA GCAGCAACAA 
CAGCTAGAGC AGCTGGGACA CCAGACACAT CTTGTGCTAG GAGCATCAAA GGAGTTGCCC 
GCGGTCAGCA GCAGCAGCGA TGAAGAGGGA TCCCTGCGGA CTCTCCACTC CAGTCGCAAG 
GTGACCAATC TGAATGCCAA GTCCACCTCT CTGCCACCCG AGGAAGGACT CTCGGGTGAT 
GTCTCCCTGG AGTCGTCCTC CAACCGACTG AATCTGCGAT TGGCCAGGAG CCTGGAGCCC 
TCAGTCCGCA ATATCACTGC ATCCGCCTCC AAGGAAGTAC AGAGCTATGA TGCTGGGGAG 
CCTGTGAATA CCAATCGATT GTTTGTGGAA CCGCCCAAGA TACCACTGTC TGCCATCCGA 
AACTCTCTTG ACGATCACGA CCTGGATATT GAGGAGTGCG ACAGCACCAC CCAGTTCGAT 
CAGGGGGATA TCTACGATAC CCTGGAACGC TGGAGCCATG GTGTCAGCTT GGGGGCACAT 
CTATTGAACT GCGCGAATGG CCACGATCAA TCTGATCCCG AGGACGACCA TCAAACCGAT 
CATCATCAGC ATGCACATCA ATCATTGCCA GTAGAACCCA AGTCTCATCA TCATCAGCAC 
CACCAGGCCA CCAATGCGCG CAGATTCATA ACGCGCACCA CGCGTTCCGC CAGTCGCCAG 
AGTTCCGCCG GACACTCCCC ACCTCCGCCG CTGCATCGCA GTGACGACTC CTCCAGCAGT 
TCACCCTCAC CGACACGCAG ACGGAATAAG CCAGGTGGTG CTCCTGCCGC CACAGATCCA 
CCATCATCGT CGGCGAACGG GAATAGTTCC GCTTATGAGA CGGCAGCCAC CACTGTGATG 
ACCCATGACC AGCAGCGAGA TCCTCCAGTT GAAGTTCCTA AGGCTCTAAA CGTCGAGCCA 
CCGCAGCCAG AAGACATCGT CCTGGGCTCC TGCCCCACTG CATCTGCCCA CTCAGATAAA 
AAGAAGGAAG AGGAGCAACG CAATCGCTAC AGTTCGCCCG TTTCACCAAA AGCCTCCAGC 
AACGGCGGAG TCCAAGCACA ACGTCGTCTT AGCAACTCGG AGCTATCGCC CAAGTCGCCA 
GGTGCCAGTG GATTCACTTC GGAGCCAGTC GCGTCAGCCA CAGCGAACAG AGTCCCGACG 
AGATCTGCCT CCCATCCTGT GGACACACCG CCCACACAAA GGCGACAGCC TCTGGTGGCC 
GTGAGTTCGG GCATCTCCAT GAAGCAGAGT CTCAGCGAGC TGCACTTCAC GGGTGGAGCA 
GTCAGTCCGC CCAAGGCTAC GGCGGAATGC ACTCTAAGGA TGCGACCACG TAGCTCCACG 
ATGAGCACCG AGAACACGGA GAATGGCCGA CGTGACCACT TGGCTAATCT GCAACTAAGT 
CCTGTGCAAA AGCAAAAGCG AACGCCCGAA GTATCACCAC GCCGTTTCGG AGATCGCGAC 
AAGTCGGCCA AACGGAAATC GAATTTGAAT CGTTCGTTGA CCGAGGAAGC CACCAAGGAT 
GGCGATGAGG TTGCCTCTTC GACCCGCCGG CGCCGACGTC GTGAACTGGG AATGGCTCGT 
CCCCTGGTTC CGGGCGATGA TGACGCCCTA AGAGATTTGC TAACGCCGCA GAAGAGTTCC 
TGCAGCGAGT CAACCAACTC CATATCCGAA CCCCATCTCC AGTTCATACC AGCCGTTCGT 
CATCCGGTGG TTCCAGTACG CACCATGACT GCTGTGCTGC CTGCACCACG ACCGCTGCAT 
ACTCCTGCGG CCCGCAACGT CAGCCAGAAG TTCAATGAAA GGACCAAAAC AGATCTGGCC 
GAGATCAAGA AGATTGCTGA GCAATCGGAG GCGCAGGTGC AACAGCCTTC GGTGGATGAT 
AACCAGAAAA TGTCAGCCAT AATGCGCAGG AAGAGCACGG ATGAGGGTGG ATCAGCTTTG 
GGGGCAACAA ACCAGAATTC ACCACCTGCC CCAAACCAAA TTGTATCCAT ACTGAAGAAG 
AAGGAACATG GACTTGGCGA GTGCAACTCC AGTGCTTCAA GCAATCCCTC GCCGGTGACC 
TTCTCTTCTA GCGTGGTGGA TCATCCTTTG GCTGGAGCTG GCCGGTGCAA ACGGCAGGGA 
ATACTCAAGA AGAGATCGAG TCTAGATGAA TCGCGCTACT ACTCCCGATC GCATTCACCC 
GACGAACGTA GTATTCTCAT TAAGTCGGCC AGGAGGAATT CCCTGGAGGA AGCTGGCACT 
GGATTGAGTC CGGCTCAGGC CCATGGAATC CTCAAGCAGA GCAGCTACGA CAGCAGCAAG 
AGCGACGGCT GCCCTTCGGC CACGGAGAGC CAGCCACACA GTATCCTGAA AAAGAAGGAT 
TCGCTATCGA CACCTTCGGA CGGAGGATGC CACAAGCATG TCTCTATATC GCAAGCTGTA 
ACTTTAGCTG CTGCTGAACT GGCTGCCCAT GATGGAATCA CCTTCGATGA TGGTGAGGAG 
CATGAGATAC GACCGATCCT AAAGCAGGAG AGCACCTCCA GCGAGGAGGC TGTGCGTCCG 
CCAAAGCCCA TACTCAAAAA GAAATCCTTT GGCGAGGCGG ATGAACACGA AATACGTCCT 
ATATTGAAGA GCTCTCGTAA AAGCAGTCGA GAGGAGTTCG ATTTGAGTGG CTTGGAAAAC 
GAGGTCAACG ATTCCCTATC GTCAATCCTA AAGACAGACT CGCCGTCCAA GCGTCGGTCG 
CTCGGTTCAG CTTTGCATGA TTTGGAGGAG TCCACCACTT CCCCGAGCAT GCTCAAGAGG 
CGCACCCGTT CGCTGGAACG TCAAGATGTC CAGCCTGGCA TGGATTTGGC AGCTGCACTG 
GATGCCATCG CCACATCACA GGCTACTCCC AGCACCCCGG TGGACTTCGT GACCACACCA 
ACAAGTATAT CGGTGGCGGA GCGGATCAAG CGCATGGAGA TGCTGTCGAC GCCCACTTCC 
CGTGCGGCGG GTGGGGCCAA CAGCAGCTGG GAATCGCCAC TGCAGGCATC GTCAGCAGAG 
CATGGAGCAC CCACCACCCC AAGGGTTCTC AAGCCAAGTG TGCTGCGCAG AGATTTGCAG 
AGGGAGCGGT ACAAAACGCA ACCCGTGACC AACGAGGAGA TGAGCTTCTT CAAAGCCAAC 
TCCGCGCCCT TCGACATCTC GGCTACATCG GCCTTCAAGC CCACCAAGCC GCTGGACATC 
CGCTTGACCC ACGCACCGCA TGCACCGCTG ATTTCGCCGA TCACCGGACA GCCACTCAGC 
CACGTGCCCG CGCCACTGCT GCTATCCTCG TCGACGAACT CGGGAGCGGG ATCCTCGTTC 
CGTCTGGCCC GCAGCTCCAC CCAACCGCTG CAGTCGCCGC GAGTTGTCCA CCTGGCCGGT 
CAGGCTACTG GTGGCTCCGC TTTGGCACGC CAGAACTCGG TGAACGACAG CATCAATCTC 
AGCGAGCAGC TAACCCTATC CATCGGCACG GACAGCGAGC ACATCTTTGA GATGTCCGCT 
TTGGAGGAGG ACTCGGCTAT TCAGTCGCTG AGCGACGAGA CAGCCGCTTC CGCATCTGCC 
AACTCAACAT CCACGACCAC TGCAACCACC ATGACACCGC CCAGTGGCCT GACGCGGAGC 
AACAGCGTTC GGGCCAGGGC CAATATGTTC CAGCAATTGC AGGAGCAGTC GCGCAACCAA 
CGGGCAACGG GAACCACCAA CTCCCGTCAA TCTCCACCAG CGTCGTCGGC GGAGGTGTCT 
TCAAGTGCAG CCGACAATTC CATGCACAGT GACGAGCGGT CCACGCCAAA TGCAACGATA 
GATGCGGAAT TCGAACCCTC ATCCCTGTCG CTGGCTGAGC GACTGGCCTT CTTCAGCAAT 
CTGTGCGAGA GCGGCGGAGG AGGTGGGAGT ACCAGTGCCG GTGGAAGGTA CCGCAGTCGC 
ACCAGCAGCT ATTCAAGGAG TCCGCCCGGC GATCGCACCA CTCCCAGGAG CAGCACCACC 
GCCAGCAGTG TCAGTATGAC GCCCACGCCC ATGGATTACT CTCCGATTCC CCACGACAAG 
GAGCCATCTA GTCTCACGCT GGAGAGCATC ATCGAGCCCG AGCAGGAGGA GGTCAAGGAG 
CAGGTGGACC AGGATGAGGT CAAGTTGAGG AAGAAGGAGC CAGTCCAGAG GGAATCTTCC 
CAGCACAATG GCTTCCACGT GCTGACCAGG TCCGCCACCG AACCGCCGTC GTCCACCTCG 
CCCCTGCGCA TCAGCATACG GACTGTGGGA AAGCTAGTCC TGCCCGATAC CTTCCGCGGC 
GATCGCAACA ATAACAGCAG TAGTAGAAGT GGGAGCAACA GCTGGAGCAA CACTTGCATG 
GTCAAGCTGC AGAGCTTGGA GCCCGTTAGC CTGTCCGTTC ATTCGCGCAC CATTGGTAAG 
GTTAAGTCGC CATTCATTGA AAAGCAGCAG GAGAAACCAC ACACCGAGAA GCTAGCGAAC 
GGAACCAGCG ACAGTGGCTC CGATTCGGGC AAGGAGAACT GCGCCTCCAA CGAGCAGGAA 
CATCTGCAGC ACCAGCAGTC GCAGGACGAG GATCGAGCTC CGCCCAGGGT GTCCGAGATG 
CGGAGGAAAA TCAGCCGACT GGTGCAACAA CAGCAGCCGC AACCACAGCC CGTCAATCGG 
CGGCACACCA CCGAGATCAC CTCCGTGACC GTTGGAACGC GTTCGCCCAG TGTGGACGAT 
CGCTTTGCCA AGTACTTTGG TGTTAAGGAG AGCTGCAACA GCACGACCAC CGTGCATAAA 
ACGCAATCGC TGCCACCCAG CCAAGTTGAG GCCACCAATG CGCACGTGAA ACTACCGCAG 
ATCACTACCG TGGTTTCCAA ACGGCGCGAG CTGCTCAAGA GGACGCGACC CCTGTCCATG 
GATCACTACT CCAGCGGCTG CACCAGTCCC ACAGCCATGC CAAGTCCCAA GAGAGCACAT 
TCGCCCGTCG GCCGGCGGAA GGCCGCCATT TCCAGCATCG AGGACATTGA GGTCACGCCG 
GATGAACTCC GGGCCGCTGG CAGGAACTTC AAGCTGTTGT ACGGCGAGTT ATTGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286916.1
CDS	776..10855
Translation	MYLIQTVQTTWRPQLPLPRELQLTNDRNSADDHHDSKVITTTTTTADIHHHNQQEENEEEDMDRLRGNHSIGGSSQQLSGKQKWSFGRLFRKKKEAESASSSEEDRKAGFVPAQRQSASKPKGKHGKGSRGCSKFDHIVVSQRQEQAPPHPPQPQIDNEFFLPLEAIQPEPYYQNQPGYYQRSSNSLDRRLLMQPGVQGNGYQQPKSRSAQRGPKPPGAHSSEEELISLNSSTFSKYRSDESIHLGSAAGQSRRSRAARNERYYKRLSRDGEPSHGGAPVVAQQRYKTQPLPLSIYQPTNASTGYVQWQPQPQKNLQNAMQNDGKRSISYDSQIHLQNMNGRMQSKPLPPPPPPRDPLRRVHVGGSGKIGSSSGDLRPVSYAFDHNSRCASDDRIWQPPHYQSVHSLNSQPSSSIVSAPVSQQQPHHRRFITRSERNANPGKQSLPNGIDFHYVADATPRSRKPIHMMDAGRMDNTGVPASSGILRTSKSGLPTPSPAPQQPLAKPRSFSSSRLSELRTYPMPMYSEVQKPKRNAPPVNPSPNQDNIVCGSLHIKPTQEHGNQNYVEIRHRDVHKTNSMPHHYQNRRSGEDLPNKYEEYVAEKREQHQQAPPPVYPERKMSLTPAVAPPPPVYFPRKKPANLEEAINELEAIYKSLGLTEPAEPKQEPKLDISSKMRVPTPSDFEKFALAHADDYEDEDSPTGEPDPIRDDVAFRNLQLANLQHRPSEKQPPFGIPVGPIVPAPQSDYLHVEPVRVKKKSGSPDIIKDDLAVRSLRKDPPGPKSSFVYPMQKKQRAIRTQSANIYNLIQRDAAKPSGGDLKSYMELTRNLERAGSMSNLQGEDGSDTKDVPATLDLLRKLKAQDEELDLARKHHPIPFRHPSQGGAIAQLPERLSKEEIPKAAPVPAPRKSLTPEPMLDDALNKIAQDAQASSIKLSQELHELRKEALITAARPKLNAEQQRLEDELQQIEAVSEAAKRCGQMLLETLPDAGQEGQEPQDKPLRKLHKEGKLIRAIDEVSEAANAVCEKILKDIVTTEPPVVVQEALQVRQTTKTGPGGEQLVMPHLIKKLDPIQSDKIEHIARRCMRQLSELANNPDYDNLATCNMSNSTTNATGGAAADATLVCPVPETQAKSQTLEEIDKIMQECERQAKSSSSTTTTDDQRTTAPSLTSGSGSGSFPPNVSTTASSFSSSSDCLAKSSSPSRASRPLSSSITSFNPYSSSDYIKSQSSDCHAPSTDPIKTFSTTSYEVQSTPQSTTISTNTMSTSQSNTISSTTNPSVESKPSAPSVSPPPLQLTPVGDRRGRNRERGASCSSTSSPSQYNSSEELAAIFGIEEQPKQQRRSRQTHNATETIAASSDLSTAPATAKTSCQPPEPLKQKPPQQQTTKDSEDSSSQKSAPVQSYRGQHPHFHHHTTPAYYTDLQLRIHTPNASDVGALNENYRSVYSTPSTQLVEQRSFPKSSTATKDPPLPISNPARGTFFRGDSASSDSHSRLRRSQQRTGSEGRFFRYSQGSVSPTKGEPPIGHEGGEQPPVVASHSPITSTSTTTISSCNPVEQASPPPTNVVNPSGSGRNSRHKQRFHSRSRRAVRVRSESRPISALYDIICKEKNLENSSSSSSSSNEQEKKKDQEQPKTTTDKLATFWPLHHHYLNPPMSSGNSGGSSGNPKQQQQQQQQQQLEQLGHQTHLVLGASKELPAVSSSSDEEGSLRTLHSSRKVTNLNAKSTSLPPEEGLSGDVSLESSSNRLNLRLARSLEPSVRNITASASKEVQSYDAGEPVNTNRLFVEPPKIPLSAIRNSLDDHDLDIEECDSTTQFDQGDIYDTLERWSHGVSLGAHLLNCANGHDQSDPEDDHQTDHHQHAHQSLPVEPKSHHHQHHQATNARRFITRTTRSASRQSSAGHSPPPPLHRSDDSSSSSPSPTRRRNKPGGAPAATDPPSSSANGNSSAYETAATTVMTHDQQRDPPVEVPKALNVEPPQPEDIVLGSCPTASAHSDKKKEEEQRNRYSSPVSPKASSNGGVQAQRRLSNSELSPKSPGASGFTSEPVASATANRVPTRSASHPVDTPPTQRRQPLVAVSSGISMKQSLSELHFTGGAVSPPKATAECTLRMRPRSSTMSTENTENGRRDHLANLQLSPVQKQKRTPEVSPRRFGDRDKSAKRKSNLNRSLTEEATKDGDEVASSTRRRRRRELGMARPLVPGDDDALRDLLTPQKSSCSESTNSISEPHLQFIPAVRHPVVPVRTMTAVLPAPRPLHTPAARNVSQKFNERTKTDLAEIKKIAEQSEAQVQQPSVDDNQKMSAIMRRKSTDEGGSALGATNQNSPPAPNQIVSILKKKEHGLGECNSSASSNPSPVTFSSSVVDHPLAGAGRCKRQGILKKRSSLDESRYYSRSHSPDERSILIKSARRNSLEEAGTGLSPAQAHGILKQSSYDSSKSDGCPSATESQPHSILKKKDSLSTPSDGGCHKHVSISQAVTLAAAELAAHDGITFDDGEEHEIRPILKQESTSSEEAVRPPKPILKKKSFGEADEHEIRPILKSSRKSSREEFDLSGLENEVNDSLSSILKTDSPSKRRSLGSALHDLEESTTSPSMLKRRTRSLERQDVQPGMDLAAALDAIATSQATPSTPVDFVTTPTSISVAERIKRMEMLSTPTSRAAGGANSSWESPLQASSAEHGAPTTPRVLKPSVLRRDLQRERYKTQPVTNEEMSFFKANSAPFDISATSAFKPTKPLDIRLTHAPHAPLISPITGQPLSHVPAPLLLSSSTNSGAGSSFRLARSSTQPLQSPRVVHLAGQATGGSALARQNSVNDSINLSEQLTLSIGTDSEHIFEMSALEEDSAIQSLSDETAASASANSTSTTTATTMTPPSGLTRSNSVRARANMFQQLQEQSRNQRATGTTNSRQSPPASSAEVSSSAADNSMHSDERSTPNATIDAEFEPSSLSLAERLAFFSNLCESGGGGGSTSAGGRYRSRTSSYSRSPPGDRTTPRSSTTASSVSMTPTPMDYSPIPHDKEPSSLTLESIIEPEQEEVKEQVDQDEVKLRKKEPVQRESSQHNGFHVLTRSATEPPSSTSPLRISIRTVGKLVLPDTFRGDRNNNSSSRSGSNSWSNTCMVKLQSLEPVSLSVHSRTIGKVKSPFIEKQQEKPHTEKLANGTSDSGSDSGKENCASNEQEHLQHQQSQDEDRAPPRVSEMRRKISRLVQQQQPQPQPVNRRHTTEITSVTVGTRSPSVDDRFAKYFGVKESCNSTTTVHKTQSLPPSQVEATNAHVKLPQITTVVSKRRELLKRTRPLSMDHYSSGCTSPTAMPSPKRAHSPVGRRKAAISSIEDIEVTPDELRAAGRNFKLLYGELLG

Target ORF information:

RefSeq Version	NM_001299987.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster supervillin, transcript variant AB (Svil), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299987.1

ORF Insert Sequence:

ATGTATCTCA TACAGACTGT TCAAACCACT TGGCGGCCAC AATTGCCGCT GCCCAGGGAG 
CTTCAGCTGA CCAATGACCG GAATAGCGCC GACGACCACC ACGATTCAAA GGTGATAACG 
ACCACCACAA CCACAGCGGA CATCCATCAC CACAACCAGC AGGAGGAGAA CGAGGAAGAG 
GATATGGATC GGCTAAGGGG CAATCACAGC ATTGGAGGAT CCTCGCAGCA GCTTTCTGGC 
AAACAGAAAT GGTCCTTCGG GCGACTTTTT CGCAAGAAAA AGGAGGCGGA GAGTGCATCC 
TCCAGCGAAG AAGATCGCAA GGCGGGATTT GTGCCTGCCC AGCGCCAGTC AGCCAGCAAG 
CCGAAGGGAA AACATGGCAA AGGATCGCGA GGTTGCAGCA AATTCGATCA CATTGTGGTG 
AGTCAGCGGC AAGAGCAGGC TCCACCACAT CCACCCCAAC CGCAAATCGA CAATGAGTTC 
TTCTTGCCCT TGGAGGCCAT ACAACCGGAG CCCTACTATC AAAATCAACC GGGCTACTAT 
CAACGATCGA GTAACTCGCT GGACAGGAGA TTACTAATGC AGCCTGGAGT TCAGGGAAAT 
GGTTACCAGC AGCCCAAATC TCGATCGGCG CAGCGAGGTC CCAAGCCACC AGGAGCTCAC 
TCCAGCGAGG AGGAACTGAT CTCGCTGAAC TCCTCCACGT TCTCCAAATA CCGCAGCGAT 
GAGAGTATTC ATTTGGGTTC CGCTGCTGGA CAAAGTCGAC GCAGTCGAGC GGCCCGCAAT 
GAGCGTTACT ACAAGAGATT GTCCCGGGAT GGCGAACCGA GTCATGGCGG AGCACCCGTT 
GTAGCCCAGC AGCGATACAA AACCCAGCCA CTGCCTTTAT CCATTTACCA GCCAACGAAC 
GCGTCCACTG GCTATGTCCA GTGGCAACCG CAGCCCCAGA AGAACCTGCA GAATGCGATG 
CAGAACGATG GAAAGCGAAG CATCAGCTAC GACAGTCAAA TACACCTGCA GAACATGAAT 
GGGAGGATGC AGAGTAAGCC ATTGCCACCG CCACCGCCTC CAAGGGATCC ACTGAGGAGA 
GTGCATGTTG GAGGTTCCGG GAAGATTGGA TCCAGCTCTG GAGACTTGCG TCCCGTGTCC 
TATGCCTTCG ATCACAATAG TCGCTGTGCG TCGGATGATC GCATTTGGCA GCCACCCCAC 
TACCAGTCGG TGCATTCGCT CAACTCCCAG CCCAGTTCGT CAATAGTCAG TGCACCAGTG 
AGTCAACAGC AACCGCACCA CAGGAGATTC ATAACTCGAT CCGAGAGGAA CGCAAATCCT 
GGCAAGCAAT CCCTGCCGAA TGGTATTGAT TTCCATTATG TGGCAGATGC AACGCCACGC 
TCCAGGAAGC CCATACACAT GATGGATGCA GGCAGGATGG ACAATACAGG AGTGCCAGCA 
TCCAGTGGTA TATTGCGAAC CTCTAAGAGT GGGCTGCCCA CACCAAGTCC TGCTCCCCAG 
CAGCCCTTGG CCAAACCGCG GTCGTTCTCC AGCTCGCGAC TTAGTGAGCT GCGAACCTAT 
CCCATGCCCA TGTACTCCGA GGTGCAGAAA CCTAAGAGGA ATGCGCCACC AGTCAATCCC 
TCGCCGAATC AGGATAACAT AGTGTGTGGG AGTCTGCACA TAAAACCCAC ACAAGAGCAT 
GGAAATCAAA ACTATGTGGA GATTCGTCAT CGGGATGTGC ACAAGACCAA CTCAATGCCG 
CACCATTATC AGAATAGGCG ATCTGGCGAG GATTTACCCA ACAAATATGA GGAGTACGTG 
GCAGAAAAGA GGGAGCAGCA TCAACAGGCA CCACCCCCAG TTTATCCGGA GCGCAAAATG 
AGCTTGACAC CAGCAGTGGC GCCACCGCCT CCTGTTTACT TCCCTCGCAA GAAGCCTGCC 
AATTTGGAGG AGGCCATCAA CGAACTGGAG GCCATTTACA AGTCTCTAGG ACTCACTGAA 
CCAGCCGAGC CCAAACAGGA ACCAAAGCTG GATATTTCCT CCAAGATGCG TGTGCCAACT 
CCCAGTGATT TTGAAAAGTT CGCCTTGGCG CATGCTGATG ACTACGAGGA TGAAGATTCG 
CCAACCGGAG AACCAGATCC AATCAGAGAT GATGTGGCCT TCAGGAACCT ACAGTTGGCC 
AATCTGCAAC ATCGGCCATC GGAGAAGCAG CCTCCATTCG GCATCCCAGT GGGACCCATT 
GTGCCAGCTC CACAGTCGGA TTATCTTCAC GTAGAGCCAG TGAGGGTGAA GAAGAAGAGT 
GGCTCTCCTG ACATCATTAA AGATGATCTT GCTGTGCGTT CATTGAGAAA GGATCCTCCT 
GGTCCCAAAT CCAGCTTTGT CTACCCAATG CAAAAGAAGC AACGGGCTAT TCGAACCCAA 
TCGGCCAACA TATACAATCT GATCCAAAGA GACGCTGCCA AGCCATCTGG TGGAGATCTT 
AAGAGCTACA TGGAGCTGAC TCGCAACCTG GAAAGAGCTG GCAGCATGTC CAATCTGCAG 
GGTGAGGATG GCAGTGATAC CAAAGATGTA CCCGCCACAC TGGATCTTCT CCGAAAGTTG 
AAAGCTCAGG ATGAGGAACT GGATTTGGCT AGAAAGCATC ACCCCATACC GTTTAGACAT 
CCCAGTCAGG GAGGAGCTAT TGCCCAACTG CCAGAACGGT TGTCCAAAGA GGAAATACCC 
AAGGCAGCGC CTGTGCCAGC TCCCCGAAAG AGTCTGACTC CTGAACCCAT GCTAGATGAT 
GCTCTCAACA AGATTGCCCA GGATGCGCAG GCCAGCAGCA TTAAGCTGAG TCAGGAACTC 
CATGAACTTC GCAAGGAGGC ATTGATTACA GCTGCGAGAC CAAAGTTGAA TGCCGAACAA 
CAGAGATTAG AGGATGAACT GCAGCAGATA GAAGCTGTCT CCGAGGCAGC CAAACGTTGT 
GGCCAAATGC TGCTCGAAAC TCTGCCAGAT GCGGGACAGG AGGGTCAGGA ACCTCAGGAT 
AAGCCCCTCA GGAAGCTACA TAAGGAGGGC AAACTCATAC GAGCTATCGA TGAGGTGTCC 
GAAGCTGCTA ATGCGGTCTG TGAGAAGATC CTCAAGGACA TTGTGACCAC AGAACCACCG 
GTGGTAGTTC AGGAGGCCCT CCAAGTGAGG CAGACCACAA AGACTGGACC AGGCGGAGAA 
CAGCTGGTGA TGCCGCATCT TATCAAGAAA CTAGATCCCA TACAATCCGA CAAGATCGAG 
CACATTGCCA GACGATGCAT GCGGCAGTTG AGCGAACTGG CCAATAATCC GGACTACGAC 
AACCTGGCCA CCTGTAATAT GAGCAACAGC ACCACCAACG CAACGGGTGG AGCAGCCGCT 
GATGCCACTC TCGTTTGTCC CGTTCCAGAG ACCCAAGCCA AGAGCCAGAC GCTCGAGGAG 
ATTGACAAGA TCATGCAGGA GTGCGAGCGT CAGGCGAAGA GCAGCAGCTC AACCACGACC 
ACCGATGATC AGCGCACCAC TGCCCCCAGT CTGACGAGTG GAAGTGGCAG CGGGAGCTTC 
CCACCCAATG TGAGCACCAC CGCCAGTTCG TTCAGCTCAA GCAGCGACTG TTTGGCCAAG 
TCGTCGAGTC CTTCGCGCGC CAGCCGTCCG CTCTCCTCGA GCATCACCTC TTTCAATCCA 
TATTCCTCCA GCGACTACAT CAAGTCGCAG AGCAGTGATT GCCATGCACC AAGCACCGAT 
CCGATCAAGA CCTTTAGCAC CACCTCGTAC GAGGTGCAGT CCACACCGCA GAGCACCACC 
ATTAGCACCA ATACGATGAG CACGAGCCAG AGCAACACGA TCAGTAGCAC TACTAATCCG 
AGTGTGGAGT CCAAGCCATC CGCCCCGTCG GTATCGCCAC CACCCCTCCA GTTGACGCCA 
GTGGGTGATC GGAGGGGCAG GAACCGGGAG CGGGGAGCTT CCTGCTCCTC CACATCCTCA 
CCTTCACAGT ACAACTCGAG CGAGGAGCTG GCCGCCATTT TTGGCATCGA GGAGCAACCG 
AAACAGCAGC GTCGCAGTCG GCAGACGCAT AATGCCACAG AAACCATTGC GGCCTCAAGT 
GACTTATCCA CAGCACCTGC CACTGCCAAG ACCAGCTGCC AGCCACCTGA GCCACTGAAA 
CAGAAGCCAC CGCAGCAGCA GACCACCAAG GACTCGGAGG ATTCCAGTTC ACAGAAGAGT 
GCTCCAGTCC AGTCGTATCG TGGTCAGCAT CCGCACTTCC ACCATCACAC CACGCCCGCT 
TACTACACGG ATCTTCAGCT GAGAATTCAC ACGCCGAACG CATCGGATGT GGGCGCCTTG 
AACGAGAACT ATCGAAGTGT ATACAGTACT CCGAGCACCC AGCTGGTGGA ACAGCGATCG 
TTTCCAAAGA GCTCCACGGC CACGAAGGAT CCACCGCTGC CCATAAGCAA TCCGGCTAGG 
GGAACATTTT TTCGTGGCGA CTCTGCGTCC AGCGACAGTC ACTCAAGACT CCGTCGGAGC 
CAGCAGAGAA CCGGCAGCGA GGGACGCTTC TTCAGATACT CTCAGGGATC TGTGAGCCCT 
ACCAAAGGTG AACCTCCAAT CGGCCACGAA GGTGGTGAGC AGCCACCCGT TGTCGCCAGT 
CACTCCCCCA TCACATCCAC CAGCACCACC ACCATCAGTA GTTGTAACCC CGTAGAGCAG 
GCTAGTCCAC CACCAACAAA CGTAGTAAAT CCCAGCGGAT CCGGTCGCAA CAGCCGGCAC 
AAGCAGCGTT TCCACAGTCG ATCCCGTCGA GCGGTGCGCG TGCGCAGTGA ATCGCGTCCG 
ATTAGCGCCC TCTATGACAT AATATGCAAG GAGAAGAACC TGGAAAACAG CAGCAGCAGC 
AGCAGCAGCA GCAACGAGCA GGAGAAGAAA AAGGATCAGG AGCAGCCTAA GACCACAACC 
GACAAGCTGG CGACCTTTTG GCCCCTGCAC CATCACTACC TCAATCCCCC GATGAGCAGC 
GGCAACTCGG GCGGCAGCAG TGGCAATCCC AAGCAGCAGC AGCAGCAGCA GCAGCAACAA 
CAGCTAGAGC AGCTGGGACA CCAGACACAT CTTGTGCTAG GAGCATCAAA GGAGTTGCCC 
GCGGTCAGCA GCAGCAGCGA TGAAGAGGGA TCCCTGCGGA CTCTCCACTC CAGTCGCAAG 
GTGACCAATC TGAATGCCAA GTCCACCTCT CTGCCACCCG AGGAAGGACT CTCGGGTGAT 
GTCTCCCTGG AGTCGTCCTC CAACCGACTG AATCTGCGAT TGGCCAGGAG CCTGGAGCCC 
TCAGTCCGCA ATATCACTGC ATCCGCCTCC AAGGAAGTAC AGAGCTATGA TGCTGGGGAG 
CCTGTGAATA CCAATCGATT GTTTGTGGAA CCGCCCAAGA TACCACTGTC TGCCATCCGA 
AACTCTCTTG ACGATCACGA CCTGGATATT GAGGAGTGCG ACAGCACCAC CCAGTTCGAT 
CAGGGGGATA TCTACGATAC CCTGGAACGC TGGAGCCATG GTGTCAGCTT GGGGGCACAT 
CTATTGAACT GCGCGAATGG CCACGATCAA TCTGATCCCG AGGACGACCA TCAAACCGAT 
CATCATCAGC ATGCACATCA ATCATTGCCA GTAGAACCCA AGTCTCATCA TCATCAGCAC 
CACCAGGCCA CCAATGCGCG CAGATTCATA ACGCGCACCA CGCGTTCCGC CAGTCGCCAG 
AGTTCCGCCG GACACTCCCC ACCTCCGCCG CTGCATCGCA GTGACGACTC CTCCAGCAGT 
TCACCCTCAC CGACACGCAG ACGGAATAAG CCAGGTGGTG CTCCTGCCGC CACAGATCCA 
CCATCATCGT CGGCGAACGG GAATAGTTCC GCTTATGAGA CGGCAGCCAC CACTGTGATG 
ACCCATGACC AGCAGCGAGA TCCTCCAGTT GAAGTTCCTA AGGCTCTAAA CGTCGAGCCA 
CCGCAGCCAG AAGACATCGT CCTGGGCTCC TGCCCCACTG CATCTGCCCA CTCAGATAAA 
AAGAAGGAAG AGGAGCAACG CAATCGCTAC AGTTCGCCCG TTTCACCAAA AGCCTCCAGC 
AACGGCGGAG TCCAAGCACA ACGTCGTCTT AGCAACTCGG AGCTATCGCC CAAGTCGCCA 
GGTGCCAGTG GATTCACTTC GGAGCCAGTC GCGTCAGCCA CAGCGAACAG AGTCCCGACG 
AGATCTGCCT CCCATCCTGT GGACACACCG CCCACACAAA GGCGACAGCC TCTGGTGGCC 
GTGAGTTCGG GCATCTCCAT GAAGCAGAGT CTCAGCGAGC TGCACTTCAC GGGTGGAGCA 
GTCAGTCCGC CCAAGGCTAC GGCGGAATGC ACTCTAAGGA TGCGACCACG TAGCTCCACG 
ATGAGCACCG AGAACACGGA GAATGGCCGA CGTGACCACT TGGCTAATCT GCAACTAAGT 
CCTGTGCAAA AGCAAAAGCG AACGCCCGAA GTATCACCAC GCCGTTTCGG AGATCGCGAC 
AAGTCGGCCA AACGGAAATC GAATTTGAAT CGTTCGTTGA CCGAGGAAGC CACCAAGGAT 
GGCGATGAGG TTGCCTCTTC GACCCGCCGG CGCCGACGTC GTGAACTGGG AATGGCTCGT 
CCCCTGGTTC CGGGCGATGA TGACGCCCTA AGAGATTTGC TAACGCCGCA GAAGAGTTCC 
TGCAGCGAGT CAACCAACTC CATATCCGAA CCCCATCTCC AGTTCATACC AGCCGTTCGT 
CATCCGGTGG TTCCAGTACG CACCATGACT GCTGTGCTGC CTGCACCACG ACCGCTGCAT 
ACTCCTGCGG CCCGCAACGT CAGCCAGAAG TTCAATGAAA GGACCAAAAC AGATCTGGCC 
GAGATCAAGA AGATTGCTGA GCAATCGGAG GCGCAGGTGC AACAGCCTTC GGTGGATGAT 
AACCAGAAAA TGTCAGCCAT AATGCGCAGG AAGAGCACGG ATGAGGGTGG ATCAGCTTTG 
GGGGCAACAA ACCAGAATTC ACCACCTGCC CCAAACCAAA TTGTATCCAT ACTGAAGAAG 
AAGGAACATG GACTTGGCGA GTGCAACTCC AGTGCTTCAA GCAATCCCTC GCCGGTGACC 
TTCTCTTCTA GCGTGGTGGA TCATCCTTTG GCTGGAGCTG GCCGGTGCAA ACGGCAGGGA 
ATACTCAAGA AGAGATCGAG TCTAGATGAA TCGCGCTACT ACTCCCGATC GCATTCACCC 
GACGAACGTA GTATTCTCAT TAAGTCGGCC AGGAGGAATT CCCTGGAGGA AGCTGGCACT 
GGATTGAGTC CGGCTCAGGC CCATGGAATC CTCAAGCAGA GCAGCTACGA CAGCAGCAAG 
AGCGACGGCT GCCCTTCGGC CACGGAGAGC CAGCCACACA GTATCCTGAA AAAGAAGGAT 
TCGCTATCGA CACCTTCGGA CGGAGGATGC CACAAGCATG TCTCTATATC GCAAGCTGTA 
ACTTTAGCTG CTGCTGAACT GGCTGCCCAT GATGGAATCA CCTTCGATGA TGGTGAGGAG 
CATGAGATAC GACCGATCCT AAAGCAGGAG AGCACCTCCA GCGAGGAGGC TGTGCGTCCG 
CCAAAGCCCA TACTCAAAAA GAAATCCTTT GGCGAGGCGG ATGAACACGA AATACGTCCT 
ATATTGAAGA GCTCTCGTAA AAGCAGTCGA GAGGAGTTCG ATTTGAGTGG CTTGGAAAAC 
GAGGTCAACG ATTCCCTATC GTCAATCCTA AAGACAGACT CGCCGTCCAA GCGTCGGTCG 
CTCGGTTCAG CTTTGCATGA TTTGGAGGAG TCCACCACTT CCCCGAGCAT GCTCAAGAGG 
CGCACCCGTT CGCTGGAACG TCAAGATGTC CAGCCTGGCA TGGATTTGGC AGCTGCACTG 
GATGCCATCG CCACATCACA GGCTACTCCC AGCACCCCGG TGGACTTCGT GACCACACCA 
ACAAGTATAT CGGTGGCGGA GCGGATCAAG CGCATGGAGA TGCTGTCGAC GCCCACTTCC 
CGTGCGGCGG GTGGGGCCAA CAGCAGCTGG GAATCGCCAC TGCAGGCATC GTCAGCAGAG 
CATGGAGCAC CCACCACCCC AAGGGTTCTC AAGCCAAGTG TGCTGCGCAG AGATTTGCAG 
AGGGAGCGGT ACAAAACGCA ACCCGTGACC AACGAGGAGA TGAGCTTCTT CAAAGCCAAC 
TCCGCGCCCT TCGACATCTC GGCTACATCG GCCTTCAAGC CCACCAAGCC GCTGGACATC 
CGCTTGACCC ACGCACCGCA TGCACCGCTG ATTTCGCCGA TCACCGGACA GCCACTCAGC 
CACGTGCCCG CGCCACTGCT GCTATCCTCG TCGACGAACT CGGGAGCGGG ATCCTCGTTC 
CGTCTGGCCC GCAGCTCCAC CCAACCGCTG CAGTCGCCGC GAGTTGTCCA CCTGGCCGGT 
CAGGCTACTG GTGGCTCCGC TTTGGCACGC CAGAACTCGG TGAACGACAG CATCAATCTC 
AGCGAGCAGC TAACCCTATC CATCGGCACG GACAGCGAGC ACATCTTTGA GATGTCCGCT 
TTGGAGGAGG ACTCGGCTAT TCAGTCGCTG AGCGACGAGA CAGCCGCTTC CGCATCTGCC 
AACTCAACAT CCACGACCAC TGCAACCACC ATGACACCGC CCAGTGGCCT GACGCGGAGC 
AACAGCGTTC GGGCCAGGGC CAATATGTTC CAGCAATTGC AGGAGCAGTC GCGCAACCAA 
CGGGCAACGG GAACCACCAA CTCCCGTCAA TCTCCACCAG CGTCGTCGGC GGAGGTGTCT 
TCAAGTGCAG CCGACAATTC CATGCACAGT GACGAGCGGT CCACGCCAAA TGCAACGATA 
GATGCGGAAT TCGAACCCTC ATCCCTGTCG CTGGCTGAGC GACTGGCCTT CTTCAGCAAT 
CTGTGCGAGA GCGGCGGAGG AGGTGGGAGT ACCAGTGCCG GTGGAAGGTA CCGCAGTCGC 
ACCAGCAGCT ATTCAAGGAG TCCGCCCGGC GATCGCACCA CTCCCAGGAG CAGCACCACC 
GCCAGCAGTG TCAGTATGAC GCCCACGCCC ATGGATTACT CTCCGATTCC CCACGACAAG 
GAGCCATCTA GTCTCACGCT GGAGAGCATC ATCGAGCCCG AGCAGGAGGA GGTCAAGGAG 
CAGGTGGACC AGGATGAGGT CAAGTTGAGG AAGAAGGAGC CAGTCCAGAG GGAATCTTCC 
CAGCACAATG GCTTCCACGT GCTGACCAGG TCCGCCACCG AACCGCCGTC GTCCACCTCG 
CCCCTGCGCA TCAGCATACG GACTGTGGGA AAGCTAGTCC TGCCCGATAC CTTCCGCGGC 
GATCGCAACA ATAACAGCAG TAGTAGAAGT GGGAGCAACA GCTGGAGCAA CACTTGCATG 
GTCAAGCTGC AGAGCTTGGA GCCCGTTAGC CTGTCCGTTC ATTCGCGCAC CATTGGTAAG 
GTTAAGTCGC CATTCATTGA AAAGCAGCAG GAGAAACCAC ACACCGAGAA GCTAGCGAAC 
GGAACCAGCG ACAGTGGCTC CGATTCGGGC AAGGAGAACT GCGCCTCCAA CGAGCAGGAA 
CATCTGCAGC ACCAGCAGTC GCAGGACGAG GATCGAGCTC CGCCCAGGGT GTCCGAGATG 
CGGAGGAAAA TCAGCCGACT GGTGCAACAA CAGCAGCCGC AACCACAGCC CGTCAATCGG 
CGGCACACCA CCGAGATCAC CTCCGTGACC GTTGGAACGC GTTCGCCCAG TGTGGACGAT 
CGCTTTGCCA AGTACTTTGG TGTTAAGGAG AGCTGCAACA GCACGACCAC CGTGCATAAA 
ACGCAATCGC TGCCACCCAG CCAAGTTGAG GCCACCAATG CGCACGTGAA ACTACCGCAG 
ATCACTACCG TGGTTTCCAA ACGGCGCGAG CTGCTCAAGA GGACGCGACC CCTGTCCATG 
GATCACTACT CCAGCGGCTG CACCAGTCCC ACAGCCATGC CAAGTCCCAA GAGAGCACAT 
TCGCCCGTCG GCCGGCGGAA GGCCGCCATT TCCAGCATCG AGGACATTGA GGTCACGCCG 
GATGAACTCC GGGCCGCTGG CAGGAACTTC AAGCTGTTGT ACGGCGAGTT ATTGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Svil ( NM_001299989.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286918.1 Drosophila melanogaster supervillin, transcript variant AD (Svil), mRNA.

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CloneID	OFa21976
Clone ID Related Accession (Same CDS sequence)	NM_001299989.1
Accession Version	NM_001299989.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	supervillin, isoform AD
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCGGC TAAGGGGCAA TCACAGCATT GGAGGATCCT CGCAGCAGCT TTCTGGCAAA 
CAGAAATGGT CCTTCGGGCG ACTTTTTCGC AAGAAAAAGG AGGCGGAGAG TGCATCCTCC 
AGCGAAGAAG ATCGCAAGGC GGGATTTGTG CCTGCCCAGC GCCAGTCAGC CAGCAAGCCG 
AAGGGAAAAC ATGGCAAAGG ATCGCGAGGT TGCAGCAAAT TCGATCACAT TGTGGTGAGT 
CAGCGGCAAG AGCAGGCTCC ACCACATCCA CCCCAACCGC AAATCGACAA TGAGTTCTTC 
TTGCCCTTGG AGGCCATACA ACCGGAGCCC TACTATCAAA ATCAACCGGG CTACTATCAA 
CGATCGAGTA ACTCGCTGGA CAGGAGATTA CTAATGCAGC CTGGAGTTCA GGGAAATGGT 
TACCAGCAGC CCAAATCTCG ATCGGCGCAG CGAGGTCCCA AGCCACCAGG AGCTCACTCC 
AGCGAGGAGG AACTGATCTC GCTGAACTCC TCCACGTTCT CCAAATACCG CAGCGATGAG 
AGTATTCATT TGGGTTCCGC TGCTGGACAA AGTCGACGCA GTCGAGCGGC CCGCAATGAG 
CGTTACTACA AGAGATTGTC CCGGGATGGC GAACCGAGTC ATGGCGGAGC ACCCGTTGTA 
GCCCAGCAGC GATACAAAAC CCAGCCACTG CCTTTATCCA TTTACCAGCC AACGAACGCG 
TCCACTGGCT ATGTCCAGTG GCAACCGCAG CCCCAGAAGA ACCTGCAGAA TGCGATGCAG 
AACGATGGAA AGCGAAGCAT CAGCTACGAC AGTCAAATAC ACCTGCAGAA CATGAATGGG 
AGGATGCAGA GTAAGCCATT GCCACCGCCA CCGCCTCCAA GGGATCCACT GAGGAGAGTG 
CATGTTGGAG GTTCCGGGAA GATTGGATCC AGCTCTGGAG ACTTGCGTCC CGTGTCCTAT 
GCCTTCGATC ACAATAGTCG CTGTGCGTCG GATGATCGCA TTTGGCAGCC ACCCCACTAC 
CAGTCGGTGC ATTCGCTCAA CTCCCAGCCC AGTTCGTCAA TAGTCAGTGC ACCAGTGAGT 
CAACAGCAAC CGCACCACAG GAGATTCATA ACTCGATCCG AGAGGAACGC AAATCCTGGC 
AAGCAATCCC TGCCGAATGG TATTGATTTC CATTATGTGG CAGATGCAAC GCCACGCTCC 
AGGAAGCCCA TACACATGAT GGATGCAGGC AGGATGGACA ATACAGGAGT GCCAGCATCC 
AGTGGTATAT TGCGAACCTC TAAGAGTGGG CTGCCCACAC CAAGTCCTGC TCCCCAGCAG 
CCCTTGGCCA AACCGCGGTC GTTCTCCAGC TCGCGACTTA GTGAGCTGCG AACCTATCCC 
ATGCCCATGT ACTCCGAGGT GCAGAAACCT AAGAGGAATG CGCCACCAGT CAATCCCTCG 
CCGAATCAGG ATAACATAGT GTGTGGGAGT CTGCACATAA AACCCACACA AGAGCATGGA 
AATCAAAACT ATGTGGAGAT TCGTCATCGG GATGTGCACA AGACCAACTC AATGCCGCAC 
CATTATCAGA ATAGGCGATC TGGCGAGGAT TTACCCAACA AATATGAGGA GTACGTGGCA 
GAAAAGAGGG AGCAGCATCA ACAGGCACCA CCCCCAGTTT ATCCGGAGCG CAAAATGAGC 
TTGACACCAG CAGTGGCGCC ACCGCCTCCT GTTTACTTCC CTCGCAAGAA GCCTGCCAAT 
TTGGAGGAGG CCATCAACGA ACTGGAGGCC ATTTACAAGT CTCTAGGACT CACTGAACCA 
GCCGAGCCCA AACAGGAACC AAAGCTGGAT ATTTCCTCCA AGATGCGTGT GCCAACTCCC 
AGTGATTTTG AAAAGTTCGC CTTGGCGCAT GCTGATGACT ACGAGGATGA AGATTCGCCA 
ACCGGAGAAC CAGATCCAAT CAGAGATGAT GTGGCCTTCA GGAACCTACA GTTGGCCAAT 
CTGCAACATC GGCCATCGGA GAAGCAGCCT CCATTCGGCA TCCCAGTGGG ACCCATTGTG 
CCAGCTCCAC AGTCGGATTA TCTTCACGTA GAGCCAGTGA GGGTGAAGAA GAAGAGTGGC 
TCTCCTGACA TCATTAAAGA TGATCTTGCT GTGCGTTCAT TGAGAAAGGA TCCTCCTGGT 
CCCAAATCCA GCTTTGTCTA CCCAATGCAA AAGAAGCAAC GGGCTATTCG AACCCAATCG 
GCCAACATAT ACAATCTGAT CCAAAGAGAC GCTGCCAAGC CATCTGGTGG AGATCTTAAG 
AGCTACATGG AGCTGACTCG CAACCTGGAA AGAGCTGGCA GCATGTCCAA TCTGCAGGGT 
GAGGATGGCA GTGATACCAA AGATGTACCC GCCACACTGG ATCTTCTCCG AAAGTTGAAA 
GCTCAGGATG AGGAACTGGA TTTGGCTAGA AAGCATCACC CCATACCGTT TAGACATCCC 
AGTCAGGGAG GAGCTATTGC CCAACTGCCA GAACGGTTGT CCAAAGAGGA AATACCCAAG 
GCAGCGCCTG TGCCAGCTCC CCGAAAGAGT CTGACTCCTG AACCCATGCT AGATGATGCT 
CTCAACAAGA TTGCCCAGGA TGCGCAGGCC AGCAGCATTA AGCTGAGTCA GGAACTCCAT 
GAACTTCGCA AGGAGGCATT GATTACAGCT GCGAGACCAA AGTTGAATGC CGAACAACAG 
AGATTAGAGG ATGAACTGCA GCAGATAGAA GCTGTCTCCG AGGCAGCCAA ACGTTGTGGC 
CAAATGCTGC TCGAAACTCT GCCAGATGCG GGACAGGAGG GTCAGGAACC TCAGGATAAG 
CCCCTCAGGA AGCTACATAA GGAGGGCAAA CTCATACGAG CTATCGATGA GGTGTCCGAA 
GCTGCTAATG CGGTCTGTGA GAAGATCCTC AAGGACATTG TGACCACAGA ACCACCGGTG 
GTAGTTCAGG AGGCCCTCCA AGTGAGGCAG ACCACAAAGA CTGGACCAGG CGGAGAACAG 
CTGGTGATGC CGCATCTTAT CAAGAAACTA GATCCCATAC AATCCGACAA GATCGAGCAC 
ATTGCCAGAC GATGCATGCG GCAGTTGAGC GAACTGGCCA ATAATCCGGA CTACGACAAC 
CTGGCCACCT GTAATATGAG CAACAGCACC ACCAACGCAA CGGGTGGAGC AGCCGCTGAT 
GCCACTCTCG TTTGTCCCGT TCCAGAGACC CAAGCCAAGA GCCAGACGCT CGAGGAGATT 
GACAAGATCA TGCAGGAGTG CGAGCGTCAG GCGAAGAGCA GCAGCTCAAC CACGACCACC 
GATGATCAGC GCACCACTGC CCCCAGTCTG ACGAGTGGAA GTGGCAGCGG GAGCTTCCCA 
CCCAATGTGA GCACCACCGC CAGTTCGTTC AGCTCAAGCA GCGACTGTTT GGCCAAGTCG 
TCGAGTCCTT CGCGCGCCAG CCGTCCGCTC TCCTCGAGCA TCACCTCTTT CAATCCATAT 
TCCTCCAGCG ACTACATCAA GTCGCAGAGC AGTGATTGCC ATGCACCAAG CACCGATCCG 
ATCAAGACCT TTAGCACCAC CTCGTACGAG GTGCAGTCCA CACCGCAGAG CACCACCATT 
AGCACCAATA CGATGAGCAC GAGCCAGAGC AACACGATCA GTAGCACTAC TAATCCGAGT 
GTGGAGTCCA AGCCATCCGC CCCGTCGGTA TCGCCACCAC CCCTCCAGTT GACGCCAGTG 
GGTGATCGGA GGGGCAGGAA CCGGGAGCGG GGAGCTTCCT GCTCCTCCAC ATCCTCACCT 
TCACAGTACA ACTCGAGCGA GGAGCTGGCC GCCATTTTTG GCATCGAGGA GCAACCGAAA 
CAGCAGCGTC GCAGTCGGCA GACGCATAAT GCCACAGAAA CCATTGCGGC CTCAAGTGAC 
TTATCCACAG CACCTGCCAC TGCCAAGACC AGCTGCCAGC CACCTGAGCC ACTGAAACAG 
AAGCCACCGC AGCAGCAGAC CACCAAGGAC TCGGAGGATT CCAGTTCACA GAAGAGTGCT 
CCAGTCCAGT CGTATCGTGG TCAGCATCCG CACTTCCACC ATCACACCAC GCCCGCTTAC 
TACACGGATC TTCAGCTGAG AATTCACACG CCGAACGCAT CGGATGTGGG CGCCTTGAAC 
GAGAACTATC GAAGTGTATA CAGTACTCCG AGCACCCAGC TGGTGGAACA GCGATCGTTT 
CCAAAGAGCT CCACGGCCAC GAAGGATCCA CCGCTGCCCA TAAGCAATCC GGCTAGGGGA 
ACATTTTTTC GTGGCGACTC TGCGTCCAGC GACAGTCACT CAAGACTCCG TCGGAGCCAG 
CAGAGAACCG GCAGCGAGGG ACGCTTCTTC AGATACTCTC AGGGATCTGT GAGCCCTACC 
AAAGGTGAAC CTCCAATCGG CCACGAAGGT GGTGAGCAGC CACCCGTTGT CGCCAGTCAC 
TCCCCCATCA CATCCACCAG CACCACCACC ATCAGTAGTT GTAACCCCGT AGAGCAGGCT 
AGTCCACCAC CAACAAACGT AGTAAATCCC AGCGGATCCG GTCGCAACAG CCGGCACAAG 
CAGCGTTTCC ACAGTCGATC CCGTCGAGCG GTGCGCGTGC GCAGTGAATC GCGTCCGATT 
AGCGCCCTCT ATGACATAAT ATGCAAGGAG AAGAACCTGG AAAACAGCAG CAGCAGCAGC 
AGCAGCAGCA ACGAGCAGGA GAAGAAAAAG GATCAGGAGC AGCCTAAGAC CACAACCGAC 
AAGCTGGCGA CCTTTTGGCC CCTGCACCAT CACTACCTCA ATCCCCCGAT GAGCAGCGGC 
AACTCGGGCG GCAGCAGTGG CAATCCCAAG CAGCAGCAGC AGCAGCAGCA GCAACAACAG 
CTAGAGCAGC TGGGACACCA GACACATCTT GTGCTAGGAG CATCAAAGGA GTTGCCCGCG 
GTCAGCAGCA GCAGCGATGA AGAGGGATCC CTGCGGACTC TCCACTCCAG TCGCAAGGTG 
ACCAATCTGA ATGCCAAGTC CACCTCTCTG CCACCCGAGG AAGGACTCTC GGGTGATGTC 
TCCCTGGAGT CGTCCTCCAA CCGACTGAAT CTGCGATTGG CCAGGAGCCT GGAGCCCTCA 
GTCCGCAATA TCACTGCATC CGCCTCCAAG GAAGTACAGA GCTATGATGC TGGGGAGCCT 
GTGAATACCA ATCGATTGTT TGTGGAACCG CCCAAGATAC CACTGTCTGC CATCCGAAAC 
TCTCTTGACG ATCACGACCT GGATATTGAG GAGTGCGACA GCACCACCCA GTTCGATCAG 
GGGGATATCT ACGATACCCT GGAACGCTGG AGCCATGGTG TCAGCTTGGG GGCACATCTA 
TTGAACTGCG CGAATGGCCA CGATCAATCT GATCCCGAGG ACGACCATCA AACCGATCAT 
CATCAGCATG CACATCAATC ATTGCCAGTA GAACCCAAGT CTCATCATCA TCAGCACCAC 
CAGGCCACCA ATGCGCGCAG ATTCATAACG CGCACCACGC GTTCCGCCAG TCGCCAGAGT 
TCCGCCGGAC ACTCCCCACC TCCGCCGCTG CATCGCAGTG ACGACTCCTC CAGCAGTTCA 
CCCTCACCGA CACGCAGACG GAATAAGCCA GGTGGTGCTC CTGCCGCCAC AGATCCACCA 
TCATCGTCGG CGAACGGGAA TAGTTCCGCT TATGAGACGG CAGCCACCAC TGTGATGACC 
CATGACCAGC AGCGAGATCC TCCAGTTGAA GTTCCTAAGG CTCTAAACGT CGAGCCACCG 
CAGCCAGAAG ACATCGTCCT GGGCTCCTGC CCCACTGCAT CTGCCCACTC AGCAGAGAGC 
CAAAGTCGAG GAAAAGCTGG CAAATGCAGT ATTAATTGCA CCTACAGCCA CAGCCACAGC 
ACCAACAACA ACTGCAACGC GCGACAACGC AACAGCAGCA ACATCAGCAG CAACAACACC 
ATCAGCAGCA ACAGCAGCAG CAGCAAAAGC ACTTGCAACA ACAATAGCAA ACAGCAACAC 
GCCACGCCCA TGCCGCCCCT AGCTAATGCC AACAAAGCCC CGTTAATTGT GCTTAAGACA 
GCCGCATCTG CAGCAGCATT GCCGTCCATT GCCGCAGCAG CAACATCCAG CGGCACGGCA 
GCAACATCTG GTGGTGTTGG TTCGCCCATC AAGCGTCGCA AGAATCCGCT GTCGCCGCTG 
CTCCTCGGCA GCCAGTGCCA GTCCACCATT GCGCCTCCGC AGGCCATTGC CAGTCCGCTC 
CTGAGCGGCG GCAGCTGCTC CTCCTCGACC ACCTCGCCCC AATCCTCGCC AGCGGTTCGC 
ACCACCAAGG CGAGTCGTCT GCGCGCCGCC GCGCTTGATA AAAAGAAGGA AGAGGAGCAA 
CGCAATCGCT ACAGTTCGCC CGTTTCACCA AAAGCCTCCA GCAACGGCGG AGTCCAAGCA 
CAACGTCGTC TTAGCAACTC GGAGCTATCG CCCAAGTCGC CAGGTGCCAG TGGATTCACT 
TCGGAGCCAG TCGCGTCAGC CACAGCGAAC AGAGTCCCGA CGAGATCTGC CTCCCATCCT 
GTGGACACAC CGCCCACACA AAGGCGACAG CCTCTGGTGG CCGTGAGTTC GGGCATCTCC 
ATGAAGCAGA GTCTCAGCGA GCTGCACTTC ACGGGTGGAG CAGTCAGTCC GCCCAAGGCT 
ACGGCGGAAT GCACTCTAAG GATGCGACCA CGTAGCTCCA CGATGAGCAC CGAGAACACG 
GAGAATGGCC GACGTGACCA CTTGGCTAAT CTGCAACTAA GTCCTGTGCA AAAGCAAAAG 
CGAACGCCCG AAGTATCACC ACGCCGTTTC GGAGATCGCG ACAAGTCGGC CAAACGGAAA 
TCGAATTTGA ATCGTTCGTT GACCGAGGAA GCCACCAAGG ATGGCGATGA GGTTGCCTCT 
TCGACCCGCC GGCGCCGACG TCGTGAACTG GGAATGGCTC GTCCCCTGGT TCCGGGCGAT 
GATGACGCCC TAAGAGATTT GCTAACGCCG CAGAAGAGTT CCTGCAGCGA GTCAACCAAC 
TCCATATCCG AACCCCATCT CCAGTTCATA CCAGCCGTTC GTCATCCGGT GGTTCCAGTA 
CGCACCATGA CTGCTGTGCT GCCTGCACCA CGACCGCTGC ATACTCCTGC GGCCCGCAAC 
GTCAGCCAGA AGTTCAATGA AAGGACCAAA ACAGATCTGG CCGAGATCAA GAAGATTGCT 
GAGCAATCGG AGGCGCAGGT GCAACAGCCT TCGGTGGATG ATAACCAGAA AATGTCAGCC 
ATAATGCGCA GGAAGAGCAC GGATGAGGGT GGATCAGCTT TGGGGGCAAC AAACCAGAAT 
TCACCACCTG CCCCAAACCA AATTGTATCC ATACTGAAGA AGAAGGAACA TGGACTTGGC 
GAGTGCAACT CCAGTGCTTC AAGCAATCCC TCGCCGGTGA CCTTCTCTTC TAGCGTGGTG 
GATCATCCTT TGGCTGGAGC TGGCCGGTGC AAACGGCAGG GAATACTCAA GAAGAGATCG 
AGTCTAGATG AATCGCGCTA CTACTCCCGA TCGCATTCAC CCGACGAACG TAGTATTCTC 
ATTAAGTCGG CCAGGAGGAA TTCCCTGGAG GAAGCTGGCA CTGGATTGAG TCCGGCTCAG 
GCCCATGGAA TCCTCAAGCA GAGCAGCTAC GACAGCAGCA AGAGCGACGG CTGCCCTTCG 
GCCACGGAGA GCCAGCCACA CAGTATCCTG AAAAAGAAGG ATTCGCTATC GACACCTTCG 
GACGGAGGAT GCCACAAGCA TGTCTCTATA TCGCAAGCTG TAACTTTAGC TGCTGCTGAA 
CTGGCTGCCC ATGATGGAAT CACCTTCGAT GATGGTGAGG AGCATGAGAT ACGACCGATC 
CTAAAGCAGG AGAGCACCTC CAGCGAGGAG GCTGTGCGTC CGCCAAAGCC CATACTCAAA 
AAGAAATCCT TTGGCGAGGC GGATGAACAC GAAATACGTC CTATATTGAA GAGCTCTCGT 
AAAAGCAGTC GAGAGGAGTT CGATTTGAGT GGCTTGGAAA ACGAGGTCAA CGATTCCCTA 
TCGTCAATCC TAAAGACAGA CTCGCCGTCC AAGCGTCGGT CGCTCGGTTC AGCTTTGCAT 
GATTTGGAGG AGTCCACCAC TTCCCCGAGC ATGCTCAAGA GGCGCACCCG TTCGCTGGAA 
CGTCAAGATG TCCAGCCTGG CATGGATTTG GCAGCTGCAC TGGATGCCAT CGCCACATCA 
CAGGCTACTC CCAGCACCCC GGTGGACTTC GTGACCACAC CAACAAGTAT ATCGGTGGCG 
GAGCGGATCA AGCGCATGGA GATGCTGTCG ACGCCCACTT CCCGTGCGGC GGGTGGGGCC 
AACAGCAGCT GGGAATCGCC ACTGCAGGCA TCGTCAGCAG AGCATGGAGC ACCCACCACC 
CCAAGGGTTC TCAAGCCAAG TGTGCTGCGC AGAGATTTGC AGAGGGAGCG GTACAAAACG 
CAACCCGTGA CCAACGAGGA GATGAGCTTC TTCAAAGCCA ACTCCGCGCC CTTCGACATC 
TCGGCTACAT CGGCCTTCAA GCCCACCAAG CCGCTGGACA TCCGCTTGAC CCACGCACCG 
CATGCACCGC TGATTTCGCC GATCACCGGA CAGCCACTCA GCCACGTGCC CGCGCCACTG 
CTGCTATCCT CGTCGACGAA CTCGGGAGCG GGATCCTCGT TCCGTCTGGC CCGCAGCTCC 
ACCCAACCGC TGCAGTCGCC GCGAGTTGTC CACCTGGCCG GTCAGGCTAC TGGTGGCTCC 
GCTTTGGCAC GCCAGAACTC GGTGAACGAC AGCATCAATC TCAGCGAGCA GCTAACCCTA 
TCCATCGGCA CGGACAGCGA GCACATCTTT GAGATGTCCG CTTTGGAGGA GGACTCGGCT 
ATTCAGTCGC TGAGCGACGA GACAGCCGCT TCCGCATCTG CCAACTCAAC ATCCACGACC 
ACTGCAACCA CCATGACACC GCCCAGTGGC CTGACGCGGA GCAACAGCGT TCGGGCCAGG 
GCCAATATGT TCCAGCAATT GCAGGAGCAG TCGCGCAACC AACGGGCAAC GGGAACCACC 
AACTCCCGTC AATCTCCACC AGCGTCGTCG GCGGAGGTGT CTTCAAGTGC AGCCGACAAT 
TCCATGCACA GTGACGAGCG GTCCACGCCA AATGCAACGA TAGATGCGGA ATTCGAACCC 
TCATCCCTGT CGCTGGCTGA GCGACTGGCC TTCTTCAGCA ATCTGTGCGA GAGCGGCGGA 
GGAGGTGGGA GTACCAGTGC CGGTGGAAGG TACCGCAGTC GCACCAGCAG CTATTCAAGG 
AGTCCGCCCG GCGATCGCAC CACTCCCAGG AGCAGCACCA CCGCCAGCAG TGTCAGTATG 
ACGCCCACGC CCATGGATTA CTCTCCGATT CCCCACGACA AGGAGCCATC TAGTCTCACG 
CTGGAGAGCA TCATCGAGCC CGAGCAGGAG GAGGTCAAGG AGCAGGTGGA CCAGGATGAG 
GTCAAGTTGA GGAAGAAGGA GCCAGTCCAG AGGGAATCTT CCCAGCACAA TGGCTTCCAC 
GTGCTGACCA GGTCCGCCAC CGAACCGCCG TCGTCCACCT CGCCCCTGCG CATCAGCATA 
CGGACTGTGG GAAAGCTAGT CCTGCCCGAT ACCTTCCGCG GCGATCGCAA CAATAACAGC 
AGTAGTAGAA GTGGGAGCAA CAGCTGGAGC AACACTTGCA TGGTCAAGCT GCAGAGCTTG 
GAGCCCGTTA GCCTGTCCGT TCATTCGCGC ACCATTGGTA AGGTTAAGTC GCCATTCATT 
GAAAAGCAGC AGGAGAAACC ACACACCGAG AAGCTAGCGA ACGGAACCAG CGACAGTGGC 
TCCGATTCGG GCAAGGAGAA CTGCGCCTCC AACGAGCAGG AACATCTGCA GCACCAGCAG 
TCGCAGGACG AGGATCGAGC TCCGCCCAGG GTGTCCGAGA TGCGGAGGAA AATCAGCCGA 
CTGGTGCAAC AACAGCAGCC GCAACCACAG CCCGTCAATC GGCGGCACAC CACCGAGATC 
ACCTCCGTGA CCGTTGGAAC GCGTTCGCCC AGTGTGGACG ATCGCTTTGC CAAGTACTTT 
GGTGTTAAGG AGAGCTGCAA CAGCACGACC ACCGTGCATA AAACGCAATC GCTGCCACCC 
AGCCAAGTTG AGGCCACCAA TGCGCACGTG AAACTACCGC AGATCACTAC CGTGGTTTCC 
AAACGGCGCG AGCTGCTCAA GAGGACGCGA CCCCTGTCCA TGGATCACTA CTCCAGCGGC 
TGCACCAGTC CCACAGCCAT GCCAAGTCCC AAGAGAGCAC ATTCGCCCGT CGGCCGGCGG 
AAGGCCGCCA TTTCCAGCAT CGAGGACATT GAGGTCACGC CGGATGAACT CCGGGCCGCT 
GGCAGGAACT TCAAGCTGTT GTACGGCGAG TTATTGGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286918.1
CDS	277..10698
Translation	MDRLRGNHSIGGSSQQLSGKQKWSFGRLFRKKKEAESASSSEEDRKAGFVPAQRQSASKPKGKHGKGSRGCSKFDHIVVSQRQEQAPPHPPQPQIDNEFFLPLEAIQPEPYYQNQPGYYQRSSNSLDRRLLMQPGVQGNGYQQPKSRSAQRGPKPPGAHSSEEELISLNSSTFSKYRSDESIHLGSAAGQSRRSRAARNERYYKRLSRDGEPSHGGAPVVAQQRYKTQPLPLSIYQPTNASTGYVQWQPQPQKNLQNAMQNDGKRSISYDSQIHLQNMNGRMQSKPLPPPPPPRDPLRRVHVGGSGKIGSSSGDLRPVSYAFDHNSRCASDDRIWQPPHYQSVHSLNSQPSSSIVSAPVSQQQPHHRRFITRSERNANPGKQSLPNGIDFHYVADATPRSRKPIHMMDAGRMDNTGVPASSGILRTSKSGLPTPSPAPQQPLAKPRSFSSSRLSELRTYPMPMYSEVQKPKRNAPPVNPSPNQDNIVCGSLHIKPTQEHGNQNYVEIRHRDVHKTNSMPHHYQNRRSGEDLPNKYEEYVAEKREQHQQAPPPVYPERKMSLTPAVAPPPPVYFPRKKPANLEEAINELEAIYKSLGLTEPAEPKQEPKLDISSKMRVPTPSDFEKFALAHADDYEDEDSPTGEPDPIRDDVAFRNLQLANLQHRPSEKQPPFGIPVGPIVPAPQSDYLHVEPVRVKKKSGSPDIIKDDLAVRSLRKDPPGPKSSFVYPMQKKQRAIRTQSANIYNLIQRDAAKPSGGDLKSYMELTRNLERAGSMSNLQGEDGSDTKDVPATLDLLRKLKAQDEELDLARKHHPIPFRHPSQGGAIAQLPERLSKEEIPKAAPVPAPRKSLTPEPMLDDALNKIAQDAQASSIKLSQELHELRKEALITAARPKLNAEQQRLEDELQQIEAVSEAAKRCGQMLLETLPDAGQEGQEPQDKPLRKLHKEGKLIRAIDEVSEAANAVCEKILKDIVTTEPPVVVQEALQVRQTTKTGPGGEQLVMPHLIKKLDPIQSDKIEHIARRCMRQLSELANNPDYDNLATCNMSNSTTNATGGAAADATLVCPVPETQAKSQTLEEIDKIMQECERQAKSSSSTTTTDDQRTTAPSLTSGSGSGSFPPNVSTTASSFSSSSDCLAKSSSPSRASRPLSSSITSFNPYSSSDYIKSQSSDCHAPSTDPIKTFSTTSYEVQSTPQSTTISTNTMSTSQSNTISSTTNPSVESKPSAPSVSPPPLQLTPVGDRRGRNRERGASCSSTSSPSQYNSSEELAAIFGIEEQPKQQRRSRQTHNATETIAASSDLSTAPATAKTSCQPPEPLKQKPPQQQTTKDSEDSSSQKSAPVQSYRGQHPHFHHHTTPAYYTDLQLRIHTPNASDVGALNENYRSVYSTPSTQLVEQRSFPKSSTATKDPPLPISNPARGTFFRGDSASSDSHSRLRRSQQRTGSEGRFFRYSQGSVSPTKGEPPIGHEGGEQPPVVASHSPITSTSTTTISSCNPVEQASPPPTNVVNPSGSGRNSRHKQRFHSRSRRAVRVRSESRPISALYDIICKEKNLENSSSSSSSSNEQEKKKDQEQPKTTTDKLATFWPLHHHYLNPPMSSGNSGGSSGNPKQQQQQQQQQQLEQLGHQTHLVLGASKELPAVSSSSDEEGSLRTLHSSRKVTNLNAKSTSLPPEEGLSGDVSLESSSNRLNLRLARSLEPSVRNITASASKEVQSYDAGEPVNTNRLFVEPPKIPLSAIRNSLDDHDLDIEECDSTTQFDQGDIYDTLERWSHGVSLGAHLLNCANGHDQSDPEDDHQTDHHQHAHQSLPVEPKSHHHQHHQATNARRFITRTTRSASRQSSAGHSPPPPLHRSDDSSSSSPSPTRRRNKPGGAPAATDPPSSSANGNSSAYETAATTVMTHDQQRDPPVEVPKALNVEPPQPEDIVLGSCPTASAHSAESQSRGKAGKCSINCTYSHSHSTNNNCNARQRNSSNISSNNTISSNSSSSKSTCNNNSKQQHATPMPPLANANKAPLIVLKTAASAAALPSIAAAATSSGTAATSGGVGSPIKRRKNPLSPLLLGSQCQSTIAPPQAIASPLLSGGSCSSSTTSPQSSPAVRTTKASRLRAAALDKKKEEEQRNRYSSPVSPKASSNGGVQAQRRLSNSELSPKSPGASGFTSEPVASATANRVPTRSASHPVDTPPTQRRQPLVAVSSGISMKQSLSELHFTGGAVSPPKATAECTLRMRPRSSTMSTENTENGRRDHLANLQLSPVQKQKRTPEVSPRRFGDRDKSAKRKSNLNRSLTEEATKDGDEVASSTRRRRRRELGMARPLVPGDDDALRDLLTPQKSSCSESTNSISEPHLQFIPAVRHPVVPVRTMTAVLPAPRPLHTPAARNVSQKFNERTKTDLAEIKKIAEQSEAQVQQPSVDDNQKMSAIMRRKSTDEGGSALGATNQNSPPAPNQIVSILKKKEHGLGECNSSASSNPSPVTFSSSVVDHPLAGAGRCKRQGILKKRSSLDESRYYSRSHSPDERSILIKSARRNSLEEAGTGLSPAQAHGILKQSSYDSSKSDGCPSATESQPHSILKKKDSLSTPSDGGCHKHVSISQAVTLAAAELAAHDGITFDDGEEHEIRPILKQESTSSEEAVRPPKPILKKKSFGEADEHEIRPILKSSRKSSREEFDLSGLENEVNDSLSSILKTDSPSKRRSLGSALHDLEESTTSPSMLKRRTRSLERQDVQPGMDLAAALDAIATSQATPSTPVDFVTTPTSISVAERIKRMEMLSTPTSRAAGGANSSWESPLQASSAEHGAPTTPRVLKPSVLRRDLQRERYKTQPVTNEEMSFFKANSAPFDISATSAFKPTKPLDIRLTHAPHAPLISPITGQPLSHVPAPLLLSSSTNSGAGSSFRLARSSTQPLQSPRVVHLAGQATGGSALARQNSVNDSINLSEQLTLSIGTDSEHIFEMSALEEDSAIQSLSDETAASASANSTSTTTATTMTPPSGLTRSNSVRARANMFQQLQEQSRNQRATGTTNSRQSPPASSAEVSSSAADNSMHSDERSTPNATIDAEFEPSSLSLAERLAFFSNLCESGGGGGSTSAGGRYRSRTSSYSRSPPGDRTTPRSSTTASSVSMTPTPMDYSPIPHDKEPSSLTLESIIEPEQEEVKEQVDQDEVKLRKKEPVQRESSQHNGFHVLTRSATEPPSSTSPLRISIRTVGKLVLPDTFRGDRNNNSSSRSGSNSWSNTCMVKLQSLEPVSLSVHSRTIGKVKSPFIEKQQEKPHTEKLANGTSDSGSDSGKENCASNEQEHLQHQQSQDEDRAPPRVSEMRRKISRLVQQQQPQPQPVNRRHTTEITSVTVGTRSPSVDDRFAKYFGVKESCNSTTTVHKTQSLPPSQVEATNAHVKLPQITTVVSKRRELLKRTRPLSMDHYSSGCTSPTAMPSPKRAHSPVGRRKAAISSIEDIEVTPDELRAAGRNFKLLYGELLG

Target ORF information:

RefSeq Version	NM_001299989.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster supervillin, transcript variant AD (Svil), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299989.1

ORF Insert Sequence:

ATGGATCGGC TAAGGGGCAA TCACAGCATT GGAGGATCCT CGCAGCAGCT TTCTGGCAAA 
CAGAAATGGT CCTTCGGGCG ACTTTTTCGC AAGAAAAAGG AGGCGGAGAG TGCATCCTCC 
AGCGAAGAAG ATCGCAAGGC GGGATTTGTG CCTGCCCAGC GCCAGTCAGC CAGCAAGCCG 
AAGGGAAAAC ATGGCAAAGG ATCGCGAGGT TGCAGCAAAT TCGATCACAT TGTGGTGAGT 
CAGCGGCAAG AGCAGGCTCC ACCACATCCA CCCCAACCGC AAATCGACAA TGAGTTCTTC 
TTGCCCTTGG AGGCCATACA ACCGGAGCCC TACTATCAAA ATCAACCGGG CTACTATCAA 
CGATCGAGTA ACTCGCTGGA CAGGAGATTA CTAATGCAGC CTGGAGTTCA GGGAAATGGT 
TACCAGCAGC CCAAATCTCG ATCGGCGCAG CGAGGTCCCA AGCCACCAGG AGCTCACTCC 
AGCGAGGAGG AACTGATCTC GCTGAACTCC TCCACGTTCT CCAAATACCG CAGCGATGAG 
AGTATTCATT TGGGTTCCGC TGCTGGACAA AGTCGACGCA GTCGAGCGGC CCGCAATGAG 
CGTTACTACA AGAGATTGTC CCGGGATGGC GAACCGAGTC ATGGCGGAGC ACCCGTTGTA 
GCCCAGCAGC GATACAAAAC CCAGCCACTG CCTTTATCCA TTTACCAGCC AACGAACGCG 
TCCACTGGCT ATGTCCAGTG GCAACCGCAG CCCCAGAAGA ACCTGCAGAA TGCGATGCAG 
AACGATGGAA AGCGAAGCAT CAGCTACGAC AGTCAAATAC ACCTGCAGAA CATGAATGGG 
AGGATGCAGA GTAAGCCATT GCCACCGCCA CCGCCTCCAA GGGATCCACT GAGGAGAGTG 
CATGTTGGAG GTTCCGGGAA GATTGGATCC AGCTCTGGAG ACTTGCGTCC CGTGTCCTAT 
GCCTTCGATC ACAATAGTCG CTGTGCGTCG GATGATCGCA TTTGGCAGCC ACCCCACTAC 
CAGTCGGTGC ATTCGCTCAA CTCCCAGCCC AGTTCGTCAA TAGTCAGTGC ACCAGTGAGT 
CAACAGCAAC CGCACCACAG GAGATTCATA ACTCGATCCG AGAGGAACGC AAATCCTGGC 
AAGCAATCCC TGCCGAATGG TATTGATTTC CATTATGTGG CAGATGCAAC GCCACGCTCC 
AGGAAGCCCA TACACATGAT GGATGCAGGC AGGATGGACA ATACAGGAGT GCCAGCATCC 
AGTGGTATAT TGCGAACCTC TAAGAGTGGG CTGCCCACAC CAAGTCCTGC TCCCCAGCAG 
CCCTTGGCCA AACCGCGGTC GTTCTCCAGC TCGCGACTTA GTGAGCTGCG AACCTATCCC 
ATGCCCATGT ACTCCGAGGT GCAGAAACCT AAGAGGAATG CGCCACCAGT CAATCCCTCG 
CCGAATCAGG ATAACATAGT GTGTGGGAGT CTGCACATAA AACCCACACA AGAGCATGGA 
AATCAAAACT ATGTGGAGAT TCGTCATCGG GATGTGCACA AGACCAACTC AATGCCGCAC 
CATTATCAGA ATAGGCGATC TGGCGAGGAT TTACCCAACA AATATGAGGA GTACGTGGCA 
GAAAAGAGGG AGCAGCATCA ACAGGCACCA CCCCCAGTTT ATCCGGAGCG CAAAATGAGC 
TTGACACCAG CAGTGGCGCC ACCGCCTCCT GTTTACTTCC CTCGCAAGAA GCCTGCCAAT 
TTGGAGGAGG CCATCAACGA ACTGGAGGCC ATTTACAAGT CTCTAGGACT CACTGAACCA 
GCCGAGCCCA AACAGGAACC AAAGCTGGAT ATTTCCTCCA AGATGCGTGT GCCAACTCCC 
AGTGATTTTG AAAAGTTCGC CTTGGCGCAT GCTGATGACT ACGAGGATGA AGATTCGCCA 
ACCGGAGAAC CAGATCCAAT CAGAGATGAT GTGGCCTTCA GGAACCTACA GTTGGCCAAT 
CTGCAACATC GGCCATCGGA GAAGCAGCCT CCATTCGGCA TCCCAGTGGG ACCCATTGTG 
CCAGCTCCAC AGTCGGATTA TCTTCACGTA GAGCCAGTGA GGGTGAAGAA GAAGAGTGGC 
TCTCCTGACA TCATTAAAGA TGATCTTGCT GTGCGTTCAT TGAGAAAGGA TCCTCCTGGT 
CCCAAATCCA GCTTTGTCTA CCCAATGCAA AAGAAGCAAC GGGCTATTCG AACCCAATCG 
GCCAACATAT ACAATCTGAT CCAAAGAGAC GCTGCCAAGC CATCTGGTGG AGATCTTAAG 
AGCTACATGG AGCTGACTCG CAACCTGGAA AGAGCTGGCA GCATGTCCAA TCTGCAGGGT 
GAGGATGGCA GTGATACCAA AGATGTACCC GCCACACTGG ATCTTCTCCG AAAGTTGAAA 
GCTCAGGATG AGGAACTGGA TTTGGCTAGA AAGCATCACC CCATACCGTT TAGACATCCC 
AGTCAGGGAG GAGCTATTGC CCAACTGCCA GAACGGTTGT CCAAAGAGGA AATACCCAAG 
GCAGCGCCTG TGCCAGCTCC CCGAAAGAGT CTGACTCCTG AACCCATGCT AGATGATGCT 
CTCAACAAGA TTGCCCAGGA TGCGCAGGCC AGCAGCATTA AGCTGAGTCA GGAACTCCAT 
GAACTTCGCA AGGAGGCATT GATTACAGCT GCGAGACCAA AGTTGAATGC CGAACAACAG 
AGATTAGAGG ATGAACTGCA GCAGATAGAA GCTGTCTCCG AGGCAGCCAA ACGTTGTGGC 
CAAATGCTGC TCGAAACTCT GCCAGATGCG GGACAGGAGG GTCAGGAACC TCAGGATAAG 
CCCCTCAGGA AGCTACATAA GGAGGGCAAA CTCATACGAG CTATCGATGA GGTGTCCGAA 
GCTGCTAATG CGGTCTGTGA GAAGATCCTC AAGGACATTG TGACCACAGA ACCACCGGTG 
GTAGTTCAGG AGGCCCTCCA AGTGAGGCAG ACCACAAAGA CTGGACCAGG CGGAGAACAG 
CTGGTGATGC CGCATCTTAT CAAGAAACTA GATCCCATAC AATCCGACAA GATCGAGCAC 
ATTGCCAGAC GATGCATGCG GCAGTTGAGC GAACTGGCCA ATAATCCGGA CTACGACAAC 
CTGGCCACCT GTAATATGAG CAACAGCACC ACCAACGCAA CGGGTGGAGC AGCCGCTGAT 
GCCACTCTCG TTTGTCCCGT TCCAGAGACC CAAGCCAAGA GCCAGACGCT CGAGGAGATT 
GACAAGATCA TGCAGGAGTG CGAGCGTCAG GCGAAGAGCA GCAGCTCAAC CACGACCACC 
GATGATCAGC GCACCACTGC CCCCAGTCTG ACGAGTGGAA GTGGCAGCGG GAGCTTCCCA 
CCCAATGTGA GCACCACCGC CAGTTCGTTC AGCTCAAGCA GCGACTGTTT GGCCAAGTCG 
TCGAGTCCTT CGCGCGCCAG CCGTCCGCTC TCCTCGAGCA TCACCTCTTT CAATCCATAT 
TCCTCCAGCG ACTACATCAA GTCGCAGAGC AGTGATTGCC ATGCACCAAG CACCGATCCG 
ATCAAGACCT TTAGCACCAC CTCGTACGAG GTGCAGTCCA CACCGCAGAG CACCACCATT 
AGCACCAATA CGATGAGCAC GAGCCAGAGC AACACGATCA GTAGCACTAC TAATCCGAGT 
GTGGAGTCCA AGCCATCCGC CCCGTCGGTA TCGCCACCAC CCCTCCAGTT GACGCCAGTG 
GGTGATCGGA GGGGCAGGAA CCGGGAGCGG GGAGCTTCCT GCTCCTCCAC ATCCTCACCT 
TCACAGTACA ACTCGAGCGA GGAGCTGGCC GCCATTTTTG GCATCGAGGA GCAACCGAAA 
CAGCAGCGTC GCAGTCGGCA GACGCATAAT GCCACAGAAA CCATTGCGGC CTCAAGTGAC 
TTATCCACAG CACCTGCCAC TGCCAAGACC AGCTGCCAGC CACCTGAGCC ACTGAAACAG 
AAGCCACCGC AGCAGCAGAC CACCAAGGAC TCGGAGGATT CCAGTTCACA GAAGAGTGCT 
CCAGTCCAGT CGTATCGTGG TCAGCATCCG CACTTCCACC ATCACACCAC GCCCGCTTAC 
TACACGGATC TTCAGCTGAG AATTCACACG CCGAACGCAT CGGATGTGGG CGCCTTGAAC 
GAGAACTATC GAAGTGTATA CAGTACTCCG AGCACCCAGC TGGTGGAACA GCGATCGTTT 
CCAAAGAGCT CCACGGCCAC GAAGGATCCA CCGCTGCCCA TAAGCAATCC GGCTAGGGGA 
ACATTTTTTC GTGGCGACTC TGCGTCCAGC GACAGTCACT CAAGACTCCG TCGGAGCCAG 
CAGAGAACCG GCAGCGAGGG ACGCTTCTTC AGATACTCTC AGGGATCTGT GAGCCCTACC 
AAAGGTGAAC CTCCAATCGG CCACGAAGGT GGTGAGCAGC CACCCGTTGT CGCCAGTCAC 
TCCCCCATCA CATCCACCAG CACCACCACC ATCAGTAGTT GTAACCCCGT AGAGCAGGCT 
AGTCCACCAC CAACAAACGT AGTAAATCCC AGCGGATCCG GTCGCAACAG CCGGCACAAG 
CAGCGTTTCC ACAGTCGATC CCGTCGAGCG GTGCGCGTGC GCAGTGAATC GCGTCCGATT 
AGCGCCCTCT ATGACATAAT ATGCAAGGAG AAGAACCTGG AAAACAGCAG CAGCAGCAGC 
AGCAGCAGCA ACGAGCAGGA GAAGAAAAAG GATCAGGAGC AGCCTAAGAC CACAACCGAC 
AAGCTGGCGA CCTTTTGGCC CCTGCACCAT CACTACCTCA ATCCCCCGAT GAGCAGCGGC 
AACTCGGGCG GCAGCAGTGG CAATCCCAAG CAGCAGCAGC AGCAGCAGCA GCAACAACAG 
CTAGAGCAGC TGGGACACCA GACACATCTT GTGCTAGGAG CATCAAAGGA GTTGCCCGCG 
GTCAGCAGCA GCAGCGATGA AGAGGGATCC CTGCGGACTC TCCACTCCAG TCGCAAGGTG 
ACCAATCTGA ATGCCAAGTC CACCTCTCTG CCACCCGAGG AAGGACTCTC GGGTGATGTC 
TCCCTGGAGT CGTCCTCCAA CCGACTGAAT CTGCGATTGG CCAGGAGCCT GGAGCCCTCA 
GTCCGCAATA TCACTGCATC CGCCTCCAAG GAAGTACAGA GCTATGATGC TGGGGAGCCT 
GTGAATACCA ATCGATTGTT TGTGGAACCG CCCAAGATAC CACTGTCTGC CATCCGAAAC 
TCTCTTGACG ATCACGACCT GGATATTGAG GAGTGCGACA GCACCACCCA GTTCGATCAG 
GGGGATATCT ACGATACCCT GGAACGCTGG AGCCATGGTG TCAGCTTGGG GGCACATCTA 
TTGAACTGCG CGAATGGCCA CGATCAATCT GATCCCGAGG ACGACCATCA AACCGATCAT 
CATCAGCATG CACATCAATC ATTGCCAGTA GAACCCAAGT CTCATCATCA TCAGCACCAC 
CAGGCCACCA ATGCGCGCAG ATTCATAACG CGCACCACGC GTTCCGCCAG TCGCCAGAGT 
TCCGCCGGAC ACTCCCCACC TCCGCCGCTG CATCGCAGTG ACGACTCCTC CAGCAGTTCA 
CCCTCACCGA CACGCAGACG GAATAAGCCA GGTGGTGCTC CTGCCGCCAC AGATCCACCA 
TCATCGTCGG CGAACGGGAA TAGTTCCGCT TATGAGACGG CAGCCACCAC TGTGATGACC 
CATGACCAGC AGCGAGATCC TCCAGTTGAA GTTCCTAAGG CTCTAAACGT CGAGCCACCG 
CAGCCAGAAG ACATCGTCCT GGGCTCCTGC CCCACTGCAT CTGCCCACTC AGCAGAGAGC 
CAAAGTCGAG GAAAAGCTGG CAAATGCAGT ATTAATTGCA CCTACAGCCA CAGCCACAGC 
ACCAACAACA ACTGCAACGC GCGACAACGC AACAGCAGCA ACATCAGCAG CAACAACACC 
ATCAGCAGCA ACAGCAGCAG CAGCAAAAGC ACTTGCAACA ACAATAGCAA ACAGCAACAC 
GCCACGCCCA TGCCGCCCCT AGCTAATGCC AACAAAGCCC CGTTAATTGT GCTTAAGACA 
GCCGCATCTG CAGCAGCATT GCCGTCCATT GCCGCAGCAG CAACATCCAG CGGCACGGCA 
GCAACATCTG GTGGTGTTGG TTCGCCCATC AAGCGTCGCA AGAATCCGCT GTCGCCGCTG 
CTCCTCGGCA GCCAGTGCCA GTCCACCATT GCGCCTCCGC AGGCCATTGC CAGTCCGCTC 
CTGAGCGGCG GCAGCTGCTC CTCCTCGACC ACCTCGCCCC AATCCTCGCC AGCGGTTCGC 
ACCACCAAGG CGAGTCGTCT GCGCGCCGCC GCGCTTGATA AAAAGAAGGA AGAGGAGCAA 
CGCAATCGCT ACAGTTCGCC CGTTTCACCA AAAGCCTCCA GCAACGGCGG AGTCCAAGCA 
CAACGTCGTC TTAGCAACTC GGAGCTATCG CCCAAGTCGC CAGGTGCCAG TGGATTCACT 
TCGGAGCCAG TCGCGTCAGC CACAGCGAAC AGAGTCCCGA CGAGATCTGC CTCCCATCCT 
GTGGACACAC CGCCCACACA AAGGCGACAG CCTCTGGTGG CCGTGAGTTC GGGCATCTCC 
ATGAAGCAGA GTCTCAGCGA GCTGCACTTC ACGGGTGGAG CAGTCAGTCC GCCCAAGGCT 
ACGGCGGAAT GCACTCTAAG GATGCGACCA CGTAGCTCCA CGATGAGCAC CGAGAACACG 
GAGAATGGCC GACGTGACCA CTTGGCTAAT CTGCAACTAA GTCCTGTGCA AAAGCAAAAG 
CGAACGCCCG AAGTATCACC ACGCCGTTTC GGAGATCGCG ACAAGTCGGC CAAACGGAAA 
TCGAATTTGA ATCGTTCGTT GACCGAGGAA GCCACCAAGG ATGGCGATGA GGTTGCCTCT 
TCGACCCGCC GGCGCCGACG TCGTGAACTG GGAATGGCTC GTCCCCTGGT TCCGGGCGAT 
GATGACGCCC TAAGAGATTT GCTAACGCCG CAGAAGAGTT CCTGCAGCGA GTCAACCAAC 
TCCATATCCG AACCCCATCT CCAGTTCATA CCAGCCGTTC GTCATCCGGT GGTTCCAGTA 
CGCACCATGA CTGCTGTGCT GCCTGCACCA CGACCGCTGC ATACTCCTGC GGCCCGCAAC 
GTCAGCCAGA AGTTCAATGA AAGGACCAAA ACAGATCTGG CCGAGATCAA GAAGATTGCT 
GAGCAATCGG AGGCGCAGGT GCAACAGCCT TCGGTGGATG ATAACCAGAA AATGTCAGCC 
ATAATGCGCA GGAAGAGCAC GGATGAGGGT GGATCAGCTT TGGGGGCAAC AAACCAGAAT 
TCACCACCTG CCCCAAACCA AATTGTATCC ATACTGAAGA AGAAGGAACA TGGACTTGGC 
GAGTGCAACT CCAGTGCTTC AAGCAATCCC TCGCCGGTGA CCTTCTCTTC TAGCGTGGTG 
GATCATCCTT TGGCTGGAGC TGGCCGGTGC AAACGGCAGG GAATACTCAA GAAGAGATCG 
AGTCTAGATG AATCGCGCTA CTACTCCCGA TCGCATTCAC CCGACGAACG TAGTATTCTC 
ATTAAGTCGG CCAGGAGGAA TTCCCTGGAG GAAGCTGGCA CTGGATTGAG TCCGGCTCAG 
GCCCATGGAA TCCTCAAGCA GAGCAGCTAC GACAGCAGCA AGAGCGACGG CTGCCCTTCG 
GCCACGGAGA GCCAGCCACA CAGTATCCTG AAAAAGAAGG ATTCGCTATC GACACCTTCG 
GACGGAGGAT GCCACAAGCA TGTCTCTATA TCGCAAGCTG TAACTTTAGC TGCTGCTGAA 
CTGGCTGCCC ATGATGGAAT CACCTTCGAT GATGGTGAGG AGCATGAGAT ACGACCGATC 
CTAAAGCAGG AGAGCACCTC CAGCGAGGAG GCTGTGCGTC CGCCAAAGCC CATACTCAAA 
AAGAAATCCT TTGGCGAGGC GGATGAACAC GAAATACGTC CTATATTGAA GAGCTCTCGT 
AAAAGCAGTC GAGAGGAGTT CGATTTGAGT GGCTTGGAAA ACGAGGTCAA CGATTCCCTA 
TCGTCAATCC TAAAGACAGA CTCGCCGTCC AAGCGTCGGT CGCTCGGTTC AGCTTTGCAT 
GATTTGGAGG AGTCCACCAC TTCCCCGAGC ATGCTCAAGA GGCGCACCCG TTCGCTGGAA 
CGTCAAGATG TCCAGCCTGG CATGGATTTG GCAGCTGCAC TGGATGCCAT CGCCACATCA 
CAGGCTACTC CCAGCACCCC GGTGGACTTC GTGACCACAC CAACAAGTAT ATCGGTGGCG 
GAGCGGATCA AGCGCATGGA GATGCTGTCG ACGCCCACTT CCCGTGCGGC GGGTGGGGCC 
AACAGCAGCT GGGAATCGCC ACTGCAGGCA TCGTCAGCAG AGCATGGAGC ACCCACCACC 
CCAAGGGTTC TCAAGCCAAG TGTGCTGCGC AGAGATTTGC AGAGGGAGCG GTACAAAACG 
CAACCCGTGA CCAACGAGGA GATGAGCTTC TTCAAAGCCA ACTCCGCGCC CTTCGACATC 
TCGGCTACAT CGGCCTTCAA GCCCACCAAG CCGCTGGACA TCCGCTTGAC CCACGCACCG 
CATGCACCGC TGATTTCGCC GATCACCGGA CAGCCACTCA GCCACGTGCC CGCGCCACTG 
CTGCTATCCT CGTCGACGAA CTCGGGAGCG GGATCCTCGT TCCGTCTGGC CCGCAGCTCC 
ACCCAACCGC TGCAGTCGCC GCGAGTTGTC CACCTGGCCG GTCAGGCTAC TGGTGGCTCC 
GCTTTGGCAC GCCAGAACTC GGTGAACGAC AGCATCAATC TCAGCGAGCA GCTAACCCTA 
TCCATCGGCA CGGACAGCGA GCACATCTTT GAGATGTCCG CTTTGGAGGA GGACTCGGCT 
ATTCAGTCGC TGAGCGACGA GACAGCCGCT TCCGCATCTG CCAACTCAAC ATCCACGACC 
ACTGCAACCA CCATGACACC GCCCAGTGGC CTGACGCGGA GCAACAGCGT TCGGGCCAGG 
GCCAATATGT TCCAGCAATT GCAGGAGCAG TCGCGCAACC AACGGGCAAC GGGAACCACC 
AACTCCCGTC AATCTCCACC AGCGTCGTCG GCGGAGGTGT CTTCAAGTGC AGCCGACAAT 
TCCATGCACA GTGACGAGCG GTCCACGCCA AATGCAACGA TAGATGCGGA ATTCGAACCC 
TCATCCCTGT CGCTGGCTGA GCGACTGGCC TTCTTCAGCA ATCTGTGCGA GAGCGGCGGA 
GGAGGTGGGA GTACCAGTGC CGGTGGAAGG TACCGCAGTC GCACCAGCAG CTATTCAAGG 
AGTCCGCCCG GCGATCGCAC CACTCCCAGG AGCAGCACCA CCGCCAGCAG TGTCAGTATG 
ACGCCCACGC CCATGGATTA CTCTCCGATT CCCCACGACA AGGAGCCATC TAGTCTCACG 
CTGGAGAGCA TCATCGAGCC CGAGCAGGAG GAGGTCAAGG AGCAGGTGGA CCAGGATGAG 
GTCAAGTTGA GGAAGAAGGA GCCAGTCCAG AGGGAATCTT CCCAGCACAA TGGCTTCCAC 
GTGCTGACCA GGTCCGCCAC CGAACCGCCG TCGTCCACCT CGCCCCTGCG CATCAGCATA 
CGGACTGTGG GAAAGCTAGT CCTGCCCGAT ACCTTCCGCG GCGATCGCAA CAATAACAGC 
AGTAGTAGAA GTGGGAGCAA CAGCTGGAGC AACACTTGCA TGGTCAAGCT GCAGAGCTTG 
GAGCCCGTTA GCCTGTCCGT TCATTCGCGC ACCATTGGTA AGGTTAAGTC GCCATTCATT 
GAAAAGCAGC AGGAGAAACC ACACACCGAG AAGCTAGCGA ACGGAACCAG CGACAGTGGC 
TCCGATTCGG GCAAGGAGAA CTGCGCCTCC AACGAGCAGG AACATCTGCA GCACCAGCAG 
TCGCAGGACG AGGATCGAGC TCCGCCCAGG GTGTCCGAGA TGCGGAGGAA AATCAGCCGA 
CTGGTGCAAC AACAGCAGCC GCAACCACAG CCCGTCAATC GGCGGCACAC CACCGAGATC 
ACCTCCGTGA CCGTTGGAAC GCGTTCGCCC AGTGTGGACG ATCGCTTTGC CAAGTACTTT 
GGTGTTAAGG AGAGCTGCAA CAGCACGACC ACCGTGCATA AAACGCAATC GCTGCCACCC 
AGCCAAGTTG AGGCCACCAA TGCGCACGTG AAACTACCGC AGATCACTAC CGTGGTTTCC 
AAACGGCGCG AGCTGCTCAA GAGGACGCGA CCCCTGTCCA TGGATCACTA CTCCAGCGGC 
TGCACCAGTC CCACAGCCAT GCCAAGTCCC AAGAGAGCAC ATTCGCCCGT CGGCCGGCGG 
AAGGCCGCCA TTTCCAGCAT CGAGGACATT GAGGTCACGC CGGATGAACT CCGGGCCGCT 
GGCAGGAACT TCAAGCTGTT GTACGGCGAG TTATTGGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Svil ( NM_001299986.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286915.1 Drosophila melanogaster supervillin, transcript variant W (Svil), mRNA.

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CloneID	OFa21977
Clone ID Related Accession (Same CDS sequence)	NM_001299986.1
Accession Version	NM_001299986.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9123bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	supervillin, isoform W
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGGCCC TCAAGGAGTG CCTCATACGG GAGGGTGTTC TCAATCTGGA CAGCCCCGAG 
AAGTACCGAC CGTACCATCT AAACGGCAGC AAAAGCAGCG GCGCCATGGA TTTTCCCCGA 
TATCGCAAAA CCATTGCGGC CTCAAGTGAC TTATCCACAG CACCTGCCAC TGCCAAGACC 
AGCTGCCAGC CACCTGAGCC ACTGAAACAG AAGCCACCGC AGCAGCAGAC CACCAAGGAC 
TCGGAGGATT CCAGTTCACA GAAGAGTGCT CCAGTCCAGT CGTATCGTGG TCAGCATCCG 
CACTTCCACC ATCACACCAC GCCCGCTTAC TACACGGATC TTCAGCTGAG AATTCACACG 
CCGAACGCAT CGGATGTGGG CGCCTTGAAC GAGAACTATC GAAGTGTATA CAGTACTCCG 
AGCACCCAGC TGGTGGAACA GCGATCGTTT CCAAAGAGCT CCACGGCCAC GAAGGATCCA 
CCGCTGCCCA TAAGCAATCC GGCTAGGGGA ACATTTTTTC GTGGCGACTC TGCGTCCAGC 
GACAGTCACT CAAGACTCCG TCGGAGCCAG CAGAGAACCG GCAGCGAGGG ACGCTTCTTC 
AGATACTCTC AGGGATCTGT GAGCCCTACC AAAGGTGAAC CTCCAATCGG CCACGAAGGT 
GGTGAGCAGC CACCCGTTGT CGCCAGTCAC TCCCCCATCA CATCCACCAG CACCACCACC 
ATCAGTAGTT GTAACCCCGT AGAGCAGGCT AGTCCACCAC CAACAAACGT AGTAAATCCC 
AGCGGATCCG GTCGCAACAG CCGGCACAAG CAGCGTTTCC ACAGTCGATC CCGTCGAGCG 
GTGCGCGTGC GCAGTGAATC GCGTCCGATT AGCGCCCTCT ATGACATAAT ATGCAAGGAG 
AAGAACCTGG AAAACAGCAG CAGCAGCAGC AGCAGCAGCA ACGAGCAGGA GAAGAAAAAG 
GATCAGGAGC AGCCTAAGAC CACAACCGAC AAGCTGGCGA CCTTTTGGCC CCTGCACCAT 
CACTACCTCA ATCCCCCGAT GAGCAGCGGC AACTCGGGCG GCAGCAGTGG CAATCCCAAG 
CAGCAGCAGC AGCAGCAGCA GCAACAACAG CTAGAGCAGC TGGGACACCA GACACATCTT 
GTGCTAGGAG CATCAAAGGA GTTGCCCGCG GTCAGCAGCA GCAGCGATGA AGAGGGATCC 
CTGCGGACTC TCCACTCCAG TCGCAAGGTG ACCAATCTGA ATGCCAAGTC CACCTCTCTG 
CCACCCGAGG AAGGACTCTC GGGTGATGTC TCCCTGGAGT CGTCCTCCAA CCGACTGAAT 
CTGCGATTGG CCAGGAGCCT GGAGCCCTCA GTCCGCAATA TCACTGCATC CGCCTCCAAG 
GAAGTACAGA GCTATGATGC TGGGGAGCCT GTGAATACCA ATCGATTGTT TGTGGAACCG 
CCCAAGATAC CACTGTCTGC CATCCGAAAC TCTCTTGACG ATCACGACCT GGATATTGAG 
GAGTGCGACA GCACCACCCA GTTCGATCAG GGGGATATCT ACGATACCCT GGAACGCTGG 
AGCCATGGTG TCAGCTTGGG GGCACATCTA TTGAACTGCG CGAATGGCCA CGATCAATCT 
GATCCCGAGG ACGACCATCA AACCGATCAT CATCAGCATG CACATCAATC ATTGCCAGTA 
GAACCCAAGT CTCATCATCA TCAGCACCAC CAGGCCACCA ATGCGCGCAG ATTCATAACG 
CGCACCACGC GTTCCGCCAG TCGCCAGAGT TCCGCCGGAC ACTCCCCACC TCCGCCGCTG 
CATCGCAGTG ACGACTCCTC CAGCAGTTCA CCCTCACCGA CACGCAGACG GAATAAGCCA 
GGTGGTGCTC CTGCCGCCAC AGATCCACCA TCATCGTCGG CGAACGGGAA TAGTTCCGCT 
TATGAGACGG CAGCCACCAC TGTGATGACC CATGACCAGC AGCGAGATCC TCCAGTTGAA 
GTTCCTAAGG CTCTAAACGT CGAGCCACCG CAGCCAGAAG ACATCGTCCT GGGCTCCTGC 
CCCACTGCAT CTGCCCACTC AGATAAAAAG AAGGAAGAGG AGCAACGCAA TCGCTACAGT 
TCGCCCGTTT CACCAAAAGC CTCCAGCAAC GGCGGAGTCC AAGCACAACG TCGTCTTAGC 
AACTCGGAGC TATCGCCCAA GTCGCCAGGT GCCAGTGGAT TCACTTCGGA GCCAGTCGCG 
TCAGCCACAG CGAACAGAGT CCCGACGAGA TCTGCCTCCC ATCCTGTGGA CACACCGCCC 
ACACAAAGGC GACAGCCTCT GGTGGCCGTG AGTTCGGGCA TCTCCATGAA GCAGAGTCTC 
AGCGAGCTGC ACTTCACGGG TGGAGCAGTC AGTCCGCCCA AGGCTACGGC GGAATGCACT 
CTAAGGATGC GACCACGTAG CTCCACGATG AGCACCGAGA ACACGGAGAA TGGCCGACGT 
GACCACTTGG CTAATCTGCA ACTAAGTCCT GTGCAAAAGC AAAAGCGAAC GCCCGAAGTA 
TCACCACGCC GTTTCGGAGA TCGCGACAAG TCGGCCAAAC GGAAATCGAA TTTGAATCGT 
TCGTTGACCG AGGAAGCCAC CAAGGATGGC GATGAGGTTG CCTCTTCGAC CCGCCGGCGC 
CGACGTCGTG AACTGGGAAT GGCTCGTCCC CTGGTTCCGG GCGATGATGA CGCCCTAAGA 
GATTTGCTAA CGCCGCAGAA GAGTTCCTGC AGCGAGTCAA CCAACTCCAT ATCCGAACCC 
CATCTCCAGT TCATACCAGC CGTTCGTCAT CCGGTGGTTC CAGTACGCAC CATGACTGCT 
GTGCTGCCTG CACCACGACC GCTGCATACT CCTGCGGCCC GCAACGTCAG CCAGAAGTTC 
AATGAAAGGA CCAAAACAGA TCTGGCCGAG ATCAAGAAGA TTGCTGAGCA ATCGGAGGCG 
CAGGTGCAAC AGCCTTCGGT GGATGATAAC CAGAAAATGT CAGCCATAAT GCGCAGGAAG 
AGCACGGATG AGGGTGGATC AGCTTTGGGG GCAACAAACC AGAATTCACC ACCTGCCCCA 
AACCAAATTG TATCCATACT GAAGAAGAAG GAACATGGAC TTGGCGAGTG CAACTCCAGT 
GCTTCAAGCA ATCCCTCGCC GGTGACCTTC TCTTCTAGCG TGGTGGATCA TCCTTTGGCT 
GGAGCTGGCC GGTGCAAACG GCAGGGAATA CTCAAGAAGA GATCGAGTCT AGATGAATCG 
CGCTACTACT CCCGATCGCA TTCACCCGAC GAACGTAGTA TTCTCATTAA GTCGGCCAGG 
AGGAATTCCC TGGAGGAAGC TGGCACTGGA TTGAGTCCGG CTCAGGCCCA TGGAATCCTC 
AAGCAGAGCA GCTACGACAG CAGCAAGAGC GACGGCTGCC CTTCGGCCAC GGAGAGCCAG 
CCACACAGTA TCCTGAAAAA GAAGGATTCG CTATCGACAC CTTCGGACGG AGGATGCCAC 
AAGCATGTCT CTATATCGCA AGCTGTAACT TTAGCTGCTG CTGAACTGGC TGCCCATGAT 
GGAATCACCT TCGATGATGG TGAGGAGCAT GAGATACGAC CGATCCTAAA GCAGGAGAGC 
ACCTCCAGCG AGGAGGCTGT GCGTCCGCCA AAGCCCATAC TCAAAAAGAA ATCCTTTGGC 
GAGGCGGATG AACACGAAAT ACGTCCTATA TTGAAGAGCT CTCGTAAAAG CAGTCGAGAG 
GAGTTCGATT TGAGTGGCTT GGAAAACGAG GTCAACGATT CCCTATCGTC AATCCTAAAG 
ACAGACTCGC CGTCCAAGCG TCGGTCGCTC GGTTCAGCTT TGCATGATTT GGAGGAGTCC 
ACCACTTCCC CGAGCATGCT CAAGAGGCGC ACCCGTTCGC TGGAACGTCA AGATGTCCAG 
CCTGGCATGG ATTTGGCAGC TGCACTGGAT GCCATCGCCA CATCACAGGC TACTCCCAGC 
ACCCCGGTGG ACTTCGTGAC CACACCAACA AGTATATCGG TGGCGGAGCG GATCAAGCGC 
ATGGAGATGC TGTCGACGCC CACTTCCCGT GCGGCGGGTG GGGCCAACAG CAGCTGGGAA 
TCGCCACTGC AGGCATCGTC AGCAGAGCAT GGAGCACCCA CCACCCCAAG GGTTCTCAAG 
CCAAGTGTGC TGCGCAGAGA TTTGCAGAGG GAGCGGTACA AAACGCAACC CGTGACCAAC 
GAGGAGATGA GCTTCTTCAA AGCCAACTCC GCGCCCTTCG ACATCTCGGC TACATCGGCC 
TTCAAGCCCA CCAAGCCGCT GGACATCCGC TTGACCCACG CACCGCATGC ACCGCTGATT 
TCGCCGATCA CCGGACAGCC ACTCAGCCAC GTGCCCGCGC CACTGCTGCT ATCCTCGTCG 
ACGAACTCGG GAGCGGGATC CTCGTTCCGT CTGGCCCGCA GCTCCACCCA ACCGCTGCAG 
TCGCCGCGAG TTGTCCACCT GGCCGGTCAG GCTACTGGTG GCTCCGCTTT GGCACGCCAG 
AACTCGGTGA ACGACAGCAT CAATCTCAGC GAGCAGCTAA CCCTATCCAT CGGCACGGAC 
AGCGAGCACA TCTTTGAGAT GTCCGCTTTG GAGGAGGACT CGGCTATTCA GTCGCTGAGC 
GACGAGACAG CCGCTTCCGC ATCTGCCAAC TCAACATCCA CGACCACTGC AACCACCATG 
ACACCGCCCA GTGGCCTGAC GCGGAGCAAC AGCGTTCGGG CCAGGGCCAA TATGTTCCAG 
CAATTGCAGG AGCAGTCGCG CAACCAACGG GCAACGGGAA CCACCAACTC CCGTCAATCT 
CCACCAGCGT CGTCGGCGGA GGTGTCTTCA AGTGCAGCCG ACAATTCCAT GCACAGTGAC 
GAGCGGTCCA CGCCAAATGC AACGATAGAT GCGGAATTCG GAAAACCCAG CACCGAACCA 
CTTAAGAGCA TTCTTAAGAC GGGAATGCCA GTGATGGGTA TGGGACGCGG TTTAATGCCT 
GTGGATCTCA ATGCCGAGCT GAAGAACCGG CTCAAACGCT CCACACATGC CACTGTTTCC 
AATCTCCGCA AATCTGCCAC CTCGGCAGAT GCCACTAGAG CAGCTAGTGA TGAGGTGGAC 
AATCCGCAGA GGAATTTAGC CCAGATCCTG AGGAATGTTT CCAAGGAGAA CTCCACGCCG 
AAGCACGATG ATGCCGTGAG CCTAGTGCGG AATCTCTCTA CTTTGGGACA ACCTCGATCG 
CTGGCCCGTG ACGATGCAGC CGGGAATTGT GCCTCGGCAT CCGAAGGCGA GAGTTCGGGG 
GGCCGCGAGA TCGAGGCCAT AATCAAGAAC AGCGCTGTGG CCAGACGTCG CCGGCAGCAA 
CAGCAGCAGT CGCAGCAGCA GAATCAGCCA GATGGCAATC TCGTTGCGAA AAGCAAAAGT 
CACTCGGGCA TATCAGCACC CATCGGCCTT GGCCCCCAGC TACCACCCCT TCCCCTCGGC 
GGCGGTTTTG TTAAGTTGCG TCATGTAGCC AAGGAGGAGG ATACCAGTAG CAGCAGCAAC 
ACATCCTCCG CCAAGGAATC GCCAGATAAG AAGGATCTTC GCCAGGAGCA GGCAGAAGCT 
TCGGGAGACT CATCGCACCC AACGGCTCTG CCACAGTTTC TTCAAGGCGT CCAACGATCA 
TCAACCGAGG TCATGGGCCC CGATCAGCGG TCTTTGGTGA TTTCGAAGAC CGGCTCCATT 
GCGGAGCGTT TGGCGGCACT CCACAAAAGT GGCGAGGACG ACTGGAAGAA GCGCATCTCG 
AAGCGCGACG AAGTCGACGA TGTGCACCGT GAAAACTTCG TAAACGAATC GCTATCTTTG 
GCCCACTCGC TGTCGGACAA ACCACTGCCC CCATCACCTT TGATACCCAC CAGCTTGGAG 
GGCGGCAAGG TCTCCGATCG TTTGAGCAAA CTCAAGTGCA ACTCGGAGAA CTGGAAGCAG 
CGCATAGAGC AAACCGATGC CAAGAAATTC ACAGTCGCCG GTCGACTGCA AAAGAAGGCC 
CAATCCCCCG TGGAGCTTCA ATTCGAGCGT TCTGCCAATG ATGCGGCCAA AAAGTGTCCC 
ATGCTGGAGG TGCGCAGCGC CAATCAGCCG CAGCTTGGGC TGGCCAAGAG CCCCTCCATG 
ATGGTCACCT CTAGCACCAG CACCAATAGC ACCAGCAAGT CCGCACTCCG CAGCCTGAGC 
GTCAATCCCG AGGAGGAGGG ACCCACCAGC CTGAGTCGCT CCAACAGCAG CAGTTCGGAG 
TCCGATGGAG AGCGCAAATC CCCCCAGAAT GCCAAGCAGA ACAAGGAGCT GGCCAACAAC 
AAGAGTGCTG CAGCACCCAC CAATGTGGGA GCCAGGATTC AGGTGCCCCG GCTGGACGAC 
AAGGAAACTT TCGAGAACTT CTTCTCCTCC AAGCCGAAGA AGGAGGAGAA CGTTCAGCTG 
GAGATCAGCG CTTTCGATGA CATCAAGCCC ACTGAGCGCT TGGTAAGCAA GCGACACGTA 
CAGGGTCCCA AAGGTCGACG GGCTGCACGC AATCCTCTCA AGAGTTTAGC TGCTCGTGAG 
GACATTAGCT CCGAGTACAC CGAAATGAGG TCGGGCATAG CCGAGCGAGA ATTACGGCGC 
CTAAAGTTGG AGTCATATGG TCATAATGCC AACTTGGCTG CAGAGGCCAT AGCCGGATTA 
GCCTCCGTCG AGGACTTCAA GTCGGTGGCA CTGCGCTCCT CGTCGATTCC ACTGCAGCAA 
ATGTGGCTAC CCCACAAGCC AGTGATGTTA CTCCATGTTA AGGGGCGCAC ACATGTCCAA 
ACCCGACTGG TGGAACCCGT GCATACCTCC CTGAACCGTG GTGATTGCTT CATCCTGGTG 
GCCGGATCGC AGCTTTTCCG CTACGTTGGA TCCTTTGCAA ACGTAATCGA GATCTCCCGG 
AGCAAGAAGA TCTGCGCTGC CATAGTGGAA AACAAGGATC TCGGTTGCAC CGCCACCCAG 
GAGGTGATCC TCACGGATGG CAAGTATTTG AAGGAGCGCC AGTGGCGTCA GTTCTGGCAG 
CTTTTGGGCA AAAAAGAGGA TGATCAATCG GATATAGCTG AATGCGGTCA TGCCGATGAA 
GACGACGTGT TCGAGAGCAG CCTGATCGAG ACTAACAAGA TCTACGAGTA CCAGGATGAG 
GCGCTCATTC CGTTGGAGAA ATTCTGGGGC TGCATCCCCA AGGTGGACAT GTTGGACACG 
CGTAAAGTTT TGGTCTTTGA CTTTGGCAGC GAGCTGTATG TGTGGAATGG CAAGAATGCC 
CCTTCCGATG CCAAGCGAGC TGCCATGAGA TTGGCCCAGG AACACTTTGG TGCTGAGAAT 
TCGGCGGACT ATGCCGACTG TTACTTGAAT CCCCTGAACT ATGTCGCCAT AGTGGGTCGT 
CGTGAGAACA CCAAGTATGC CAAGAGGTGC GCACAGCGTC CAGAATGGTG TGTTCTCGGC 
AAGATCACCC AGAACATGGA GACGGTGCTG TTCAAGGAGA AGTTCAGTGA TTGGCCAGAG 
ATAGAACGCG AGGATCTGGA GAAGGATTAC CTATCAAACG GAGTGCATAT GGTGCGTGCT 
TTAAACGGCA CCGCTTTGCA TAAGGGAGAA CCCTACCAGG AACCAAATCT GGTGCTCGAG 
CAGGCCAACT TGGGGCGTGG CAATTTCTAC TACGACACGG ACACCATGCG GCACTTCGAT 
GTGATTACCA AGTCAACGGA CAAATGGCAG ATCCACGAGT TCAACTTTGA TGCGGCGAAT 
AATCGCGATG ACTACGGTCA CTTCTACTCG GCGGAGAGCT ATATAGTGCG CTGGATCTAC 
CAGATTTCGG TCACAGTTCG CGAACTCAGT GGCAAGGTGT CGAACAGAAG CACAGTGGGT 
CGAGACAGGT GTGTCTATTT CACCTGGCAA GGACAGGACT CAAGTGCCAA CGAGAAGGGT 
GCAGCTGCTT TGCTGACCGT AGAGTTGGAC AAGGAGAAAG GCGCACAGAT GCGGGTGTCG 
CAAGGAGATG AGTGCACAGC CTTCGTCCGC CTGTTCCGGC AAATGTGGCA ACATCGTGGA 
CGCAAGGAGC AATGTCTGGA GAGGCACAGC GAGTGGAGGC TCTACCAACT GCAGGGCAAC 
GTCAGCGAGG AGACCATCAT CAAGGAAGTC GTCTGCCAGT CCAGCCATTT GCGATCCCGC 
AGCTCCATGC TGTTAATTCA CGGCAGTCAG GGAAATGTGA TTGTCTGGCA TGGATCGAAG 
AGTGCTCCTC ATACGCGTAA TGTGGCTGTA GGAGTTGCTC AGGATCTGAT CAAGACGGTG 
CCTAAAGATC TCTTCAGCGC CGAAACTGCC AACCTAACTG AGGTGGAGGA GGGTTCCGAC 
GACGATCAGT GCAAGAAGGC CCTGGGATTG AAGGATGAAA GAAATGAATA TGGCAGCCTG 
CTTAAGTCCA CCAAGTCGTT CGATTACCAA ATAAGGATCT TCAACTTCTC CAGCACTCAG 
GGTGTTTTCA AAGCCCTCGA ACTGAGCGAT CCGCTGCGCT GCCAGGATCT GCATAGTCCG 
TATCCCTTCA GCCAGTCGCA GCTGTACAAT GCAAGGCAGC CGACTATCTT TTTGCTGGAC 
GACGGCGATG AGTTGTGGCT CTGGATGGGA TGGTGGCCTC TGGAAGATGT GAAGATAAAC 
ACCGATGAGC GAAGCAGTCC CACCAACGAA AATCGCGCTG GAGTAAATCG CTGGATCTCA 
GAGAGACGTG CTGCCCTCGA GACAGCCGTT GACTATTGGC GTGCCAAGCA CGGCGAGAAC 
GAGAATGAAC CCTTCCATGG CATCAAAGGC CAGGTTGTGT GGGCAGGCCT GGAGCCACTG 
GCCTTTAAGG CGCTGTTTCC CGACTGGACA GAACGTCCGG ATGTAAGAGA AATCAACGAA 
GAAGATGGGC GAACTGATGC TTCCATATCG ATCGCCACAA TGCTGGCACA AATGACGCAG 
ACCGAGTATC CCCTGGAAGT CCTGAAAGCC CGACCGCTGC CCGAAGGTGT GGAGCCCACC 
CGCCTGGAGG TCTACCTTAA TGCGGAGGAC TTCCAGGCGG CACTGGGATG CAGCCGGGCG 
GAATTCGAGC AGCTGCCCAT CTGGAAACAG ACCAAGCTGA AGAAGGAGCG AGGGCTGTTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286915.1
CDS	394..9516
Translation	MLALKECLIREGVLNLDSPEKYRPYHLNGSKSSGAMDFPRYRKTIAASSDLSTAPATAKTSCQPPEPLKQKPPQQQTTKDSEDSSSQKSAPVQSYRGQHPHFHHHTTPAYYTDLQLRIHTPNASDVGALNENYRSVYSTPSTQLVEQRSFPKSSTATKDPPLPISNPARGTFFRGDSASSDSHSRLRRSQQRTGSEGRFFRYSQGSVSPTKGEPPIGHEGGEQPPVVASHSPITSTSTTTISSCNPVEQASPPPTNVVNPSGSGRNSRHKQRFHSRSRRAVRVRSESRPISALYDIICKEKNLENSSSSSSSSNEQEKKKDQEQPKTTTDKLATFWPLHHHYLNPPMSSGNSGGSSGNPKQQQQQQQQQQLEQLGHQTHLVLGASKELPAVSSSSDEEGSLRTLHSSRKVTNLNAKSTSLPPEEGLSGDVSLESSSNRLNLRLARSLEPSVRNITASASKEVQSYDAGEPVNTNRLFVEPPKIPLSAIRNSLDDHDLDIEECDSTTQFDQGDIYDTLERWSHGVSLGAHLLNCANGHDQSDPEDDHQTDHHQHAHQSLPVEPKSHHHQHHQATNARRFITRTTRSASRQSSAGHSPPPPLHRSDDSSSSSPSPTRRRNKPGGAPAATDPPSSSANGNSSAYETAATTVMTHDQQRDPPVEVPKALNVEPPQPEDIVLGSCPTASAHSDKKKEEEQRNRYSSPVSPKASSNGGVQAQRRLSNSELSPKSPGASGFTSEPVASATANRVPTRSASHPVDTPPTQRRQPLVAVSSGISMKQSLSELHFTGGAVSPPKATAECTLRMRPRSSTMSTENTENGRRDHLANLQLSPVQKQKRTPEVSPRRFGDRDKSAKRKSNLNRSLTEEATKDGDEVASSTRRRRRRELGMARPLVPGDDDALRDLLTPQKSSCSESTNSISEPHLQFIPAVRHPVVPVRTMTAVLPAPRPLHTPAARNVSQKFNERTKTDLAEIKKIAEQSEAQVQQPSVDDNQKMSAIMRRKSTDEGGSALGATNQNSPPAPNQIVSILKKKEHGLGECNSSASSNPSPVTFSSSVVDHPLAGAGRCKRQGILKKRSSLDESRYYSRSHSPDERSILIKSARRNSLEEAGTGLSPAQAHGILKQSSYDSSKSDGCPSATESQPHSILKKKDSLSTPSDGGCHKHVSISQAVTLAAAELAAHDGITFDDGEEHEIRPILKQESTSSEEAVRPPKPILKKKSFGEADEHEIRPILKSSRKSSREEFDLSGLENEVNDSLSSILKTDSPSKRRSLGSALHDLEESTTSPSMLKRRTRSLERQDVQPGMDLAAALDAIATSQATPSTPVDFVTTPTSISVAERIKRMEMLSTPTSRAAGGANSSWESPLQASSAEHGAPTTPRVLKPSVLRRDLQRERYKTQPVTNEEMSFFKANSAPFDISATSAFKPTKPLDIRLTHAPHAPLISPITGQPLSHVPAPLLLSSSTNSGAGSSFRLARSSTQPLQSPRVVHLAGQATGGSALARQNSVNDSINLSEQLTLSIGTDSEHIFEMSALEEDSAIQSLSDETAASASANSTSTTTATTMTPPSGLTRSNSVRARANMFQQLQEQSRNQRATGTTNSRQSPPASSAEVSSSAADNSMHSDERSTPNATIDAEFGKPSTEPLKSILKTGMPVMGMGRGLMPVDLNAELKNRLKRSTHATVSNLRKSATSADATRAASDEVDNPQRNLAQILRNVSKENSTPKHDDAVSLVRNLSTLGQPRSLARDDAAGNCASASEGESSGGREIEAIIKNSAVARRRRQQQQQSQQQNQPDGNLVAKSKSHSGISAPIGLGPQLPPLPLGGGFVKLRHVAKEEDTSSSSNTSSAKESPDKKDLRQEQAEASGDSSHPTALPQFLQGVQRSSTEVMGPDQRSLVISKTGSIAERLAALHKSGEDDWKKRISKRDEVDDVHRENFVNESLSLAHSLSDKPLPPSPLIPTSLEGGKVSDRLSKLKCNSENWKQRIEQTDAKKFTVAGRLQKKAQSPVELQFERSANDAAKKCPMLEVRSANQPQLGLAKSPSMMVTSSTSTNSTSKSALRSLSVNPEEEGPTSLSRSNSSSSESDGERKSPQNAKQNKELANNKSAAAPTNVGARIQVPRLDDKETFENFFSSKPKKEENVQLEISAFDDIKPTERLVSKRHVQGPKGRRAARNPLKSLAAREDISSEYTEMRSGIAERELRRLKLESYGHNANLAAEAIAGLASVEDFKSVALRSSSIPLQQMWLPHKPVMLLHVKGRTHVQTRLVEPVHTSLNRGDCFILVAGSQLFRYVGSFANVIEISRSKKICAAIVENKDLGCTATQEVILTDGKYLKERQWRQFWQLLGKKEDDQSDIAECGHADEDDVFESSLIETNKIYEYQDEALIPLEKFWGCIPKVDMLDTRKVLVFDFGSELYVWNGKNAPSDAKRAAMRLAQEHFGAENSADYADCYLNPLNYVAIVGRRENTKYAKRCAQRPEWCVLGKITQNMETVLFKEKFSDWPEIEREDLEKDYLSNGVHMVRALNGTALHKGEPYQEPNLVLEQANLGRGNFYYDTDTMRHFDVITKSTDKWQIHEFNFDAANNRDDYGHFYSAESYIVRWIYQISVTVRELSGKVSNRSTVGRDRCVYFTWQGQDSSANEKGAAALLTVELDKEKGAQMRVSQGDECTAFVRLFRQMWQHRGRKEQCLERHSEWRLYQLQGNVSEETIIKEVVCQSSHLRSRSSMLLIHGSQGNVIVWHGSKSAPHTRNVAVGVAQDLIKTVPKDLFSAETANLTEVEEGSDDDQCKKALGLKDERNEYGSLLKSTKSFDYQIRIFNFSSTQGVFKALELSDPLRCQDLHSPYPFSQSQLYNARQPTIFLLDDGDELWLWMGWWPLEDVKINTDERSSPTNENRAGVNRWISERRAALETAVDYWRAKHGENENEPFHGIKGQVVWAGLEPLAFKALFPDWTERPDVREINEEDGRTDASISIATMLAQMTQTEYPLEVLKARPLPEGVEPTRLEVYLNAEDFQAALGCSRAEFEQLPIWKQTKLKKERGLF

Target ORF information:

RefSeq Version	NM_001299986.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster supervillin, transcript variant W (Svil), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299986.1

ORF Insert Sequence:

ATGCTGGCCC TCAAGGAGTG CCTCATACGG GAGGGTGTTC TCAATCTGGA CAGCCCCGAG 
AAGTACCGAC CGTACCATCT AAACGGCAGC AAAAGCAGCG GCGCCATGGA TTTTCCCCGA 
TATCGCAAAA CCATTGCGGC CTCAAGTGAC TTATCCACAG CACCTGCCAC TGCCAAGACC 
AGCTGCCAGC CACCTGAGCC ACTGAAACAG AAGCCACCGC AGCAGCAGAC CACCAAGGAC 
TCGGAGGATT CCAGTTCACA GAAGAGTGCT CCAGTCCAGT CGTATCGTGG TCAGCATCCG 
CACTTCCACC ATCACACCAC GCCCGCTTAC TACACGGATC TTCAGCTGAG AATTCACACG 
CCGAACGCAT CGGATGTGGG CGCCTTGAAC GAGAACTATC GAAGTGTATA CAGTACTCCG 
AGCACCCAGC TGGTGGAACA GCGATCGTTT CCAAAGAGCT CCACGGCCAC GAAGGATCCA 
CCGCTGCCCA TAAGCAATCC GGCTAGGGGA ACATTTTTTC GTGGCGACTC TGCGTCCAGC 
GACAGTCACT CAAGACTCCG TCGGAGCCAG CAGAGAACCG GCAGCGAGGG ACGCTTCTTC 
AGATACTCTC AGGGATCTGT GAGCCCTACC AAAGGTGAAC CTCCAATCGG CCACGAAGGT 
GGTGAGCAGC CACCCGTTGT CGCCAGTCAC TCCCCCATCA CATCCACCAG CACCACCACC 
ATCAGTAGTT GTAACCCCGT AGAGCAGGCT AGTCCACCAC CAACAAACGT AGTAAATCCC 
AGCGGATCCG GTCGCAACAG CCGGCACAAG CAGCGTTTCC ACAGTCGATC CCGTCGAGCG 
GTGCGCGTGC GCAGTGAATC GCGTCCGATT AGCGCCCTCT ATGACATAAT ATGCAAGGAG 
AAGAACCTGG AAAACAGCAG CAGCAGCAGC AGCAGCAGCA ACGAGCAGGA GAAGAAAAAG 
GATCAGGAGC AGCCTAAGAC CACAACCGAC AAGCTGGCGA CCTTTTGGCC CCTGCACCAT 
CACTACCTCA ATCCCCCGAT GAGCAGCGGC AACTCGGGCG GCAGCAGTGG CAATCCCAAG 
CAGCAGCAGC AGCAGCAGCA GCAACAACAG CTAGAGCAGC TGGGACACCA GACACATCTT 
GTGCTAGGAG CATCAAAGGA GTTGCCCGCG GTCAGCAGCA GCAGCGATGA AGAGGGATCC 
CTGCGGACTC TCCACTCCAG TCGCAAGGTG ACCAATCTGA ATGCCAAGTC CACCTCTCTG 
CCACCCGAGG AAGGACTCTC GGGTGATGTC TCCCTGGAGT CGTCCTCCAA CCGACTGAAT 
CTGCGATTGG CCAGGAGCCT GGAGCCCTCA GTCCGCAATA TCACTGCATC CGCCTCCAAG 
GAAGTACAGA GCTATGATGC TGGGGAGCCT GTGAATACCA ATCGATTGTT TGTGGAACCG 
CCCAAGATAC CACTGTCTGC CATCCGAAAC TCTCTTGACG ATCACGACCT GGATATTGAG 
GAGTGCGACA GCACCACCCA GTTCGATCAG GGGGATATCT ACGATACCCT GGAACGCTGG 
AGCCATGGTG TCAGCTTGGG GGCACATCTA TTGAACTGCG CGAATGGCCA CGATCAATCT 
GATCCCGAGG ACGACCATCA AACCGATCAT CATCAGCATG CACATCAATC ATTGCCAGTA 
GAACCCAAGT CTCATCATCA TCAGCACCAC CAGGCCACCA ATGCGCGCAG ATTCATAACG 
CGCACCACGC GTTCCGCCAG TCGCCAGAGT TCCGCCGGAC ACTCCCCACC TCCGCCGCTG 
CATCGCAGTG ACGACTCCTC CAGCAGTTCA CCCTCACCGA CACGCAGACG GAATAAGCCA 
GGTGGTGCTC CTGCCGCCAC AGATCCACCA TCATCGTCGG CGAACGGGAA TAGTTCCGCT 
TATGAGACGG CAGCCACCAC TGTGATGACC CATGACCAGC AGCGAGATCC TCCAGTTGAA 
GTTCCTAAGG CTCTAAACGT CGAGCCACCG CAGCCAGAAG ACATCGTCCT GGGCTCCTGC 
CCCACTGCAT CTGCCCACTC AGATAAAAAG AAGGAAGAGG AGCAACGCAA TCGCTACAGT 
TCGCCCGTTT CACCAAAAGC CTCCAGCAAC GGCGGAGTCC AAGCACAACG TCGTCTTAGC 
AACTCGGAGC TATCGCCCAA GTCGCCAGGT GCCAGTGGAT TCACTTCGGA GCCAGTCGCG 
TCAGCCACAG CGAACAGAGT CCCGACGAGA TCTGCCTCCC ATCCTGTGGA CACACCGCCC 
ACACAAAGGC GACAGCCTCT GGTGGCCGTG AGTTCGGGCA TCTCCATGAA GCAGAGTCTC 
AGCGAGCTGC ACTTCACGGG TGGAGCAGTC AGTCCGCCCA AGGCTACGGC GGAATGCACT 
CTAAGGATGC GACCACGTAG CTCCACGATG AGCACCGAGA ACACGGAGAA TGGCCGACGT 
GACCACTTGG CTAATCTGCA ACTAAGTCCT GTGCAAAAGC AAAAGCGAAC GCCCGAAGTA 
TCACCACGCC GTTTCGGAGA TCGCGACAAG TCGGCCAAAC GGAAATCGAA TTTGAATCGT 
TCGTTGACCG AGGAAGCCAC CAAGGATGGC GATGAGGTTG CCTCTTCGAC CCGCCGGCGC 
CGACGTCGTG AACTGGGAAT GGCTCGTCCC CTGGTTCCGG GCGATGATGA CGCCCTAAGA 
GATTTGCTAA CGCCGCAGAA GAGTTCCTGC AGCGAGTCAA CCAACTCCAT ATCCGAACCC 
CATCTCCAGT TCATACCAGC CGTTCGTCAT CCGGTGGTTC CAGTACGCAC CATGACTGCT 
GTGCTGCCTG CACCACGACC GCTGCATACT CCTGCGGCCC GCAACGTCAG CCAGAAGTTC 
AATGAAAGGA CCAAAACAGA TCTGGCCGAG ATCAAGAAGA TTGCTGAGCA ATCGGAGGCG 
CAGGTGCAAC AGCCTTCGGT GGATGATAAC CAGAAAATGT CAGCCATAAT GCGCAGGAAG 
AGCACGGATG AGGGTGGATC AGCTTTGGGG GCAACAAACC AGAATTCACC ACCTGCCCCA 
AACCAAATTG TATCCATACT GAAGAAGAAG GAACATGGAC TTGGCGAGTG CAACTCCAGT 
GCTTCAAGCA ATCCCTCGCC GGTGACCTTC TCTTCTAGCG TGGTGGATCA TCCTTTGGCT 
GGAGCTGGCC GGTGCAAACG GCAGGGAATA CTCAAGAAGA GATCGAGTCT AGATGAATCG 
CGCTACTACT CCCGATCGCA TTCACCCGAC GAACGTAGTA TTCTCATTAA GTCGGCCAGG 
AGGAATTCCC TGGAGGAAGC TGGCACTGGA TTGAGTCCGG CTCAGGCCCA TGGAATCCTC 
AAGCAGAGCA GCTACGACAG CAGCAAGAGC GACGGCTGCC CTTCGGCCAC GGAGAGCCAG 
CCACACAGTA TCCTGAAAAA GAAGGATTCG CTATCGACAC CTTCGGACGG AGGATGCCAC 
AAGCATGTCT CTATATCGCA AGCTGTAACT TTAGCTGCTG CTGAACTGGC TGCCCATGAT 
GGAATCACCT TCGATGATGG TGAGGAGCAT GAGATACGAC CGATCCTAAA GCAGGAGAGC 
ACCTCCAGCG AGGAGGCTGT GCGTCCGCCA AAGCCCATAC TCAAAAAGAA ATCCTTTGGC 
GAGGCGGATG AACACGAAAT ACGTCCTATA TTGAAGAGCT CTCGTAAAAG CAGTCGAGAG 
GAGTTCGATT TGAGTGGCTT GGAAAACGAG GTCAACGATT CCCTATCGTC AATCCTAAAG 
ACAGACTCGC CGTCCAAGCG TCGGTCGCTC GGTTCAGCTT TGCATGATTT GGAGGAGTCC 
ACCACTTCCC CGAGCATGCT CAAGAGGCGC ACCCGTTCGC TGGAACGTCA AGATGTCCAG 
CCTGGCATGG ATTTGGCAGC TGCACTGGAT GCCATCGCCA CATCACAGGC TACTCCCAGC 
ACCCCGGTGG ACTTCGTGAC CACACCAACA AGTATATCGG TGGCGGAGCG GATCAAGCGC 
ATGGAGATGC TGTCGACGCC CACTTCCCGT GCGGCGGGTG GGGCCAACAG CAGCTGGGAA 
TCGCCACTGC AGGCATCGTC AGCAGAGCAT GGAGCACCCA CCACCCCAAG GGTTCTCAAG 
CCAAGTGTGC TGCGCAGAGA TTTGCAGAGG GAGCGGTACA AAACGCAACC CGTGACCAAC 
GAGGAGATGA GCTTCTTCAA AGCCAACTCC GCGCCCTTCG ACATCTCGGC TACATCGGCC 
TTCAAGCCCA CCAAGCCGCT GGACATCCGC TTGACCCACG CACCGCATGC ACCGCTGATT 
TCGCCGATCA CCGGACAGCC ACTCAGCCAC GTGCCCGCGC CACTGCTGCT ATCCTCGTCG 
ACGAACTCGG GAGCGGGATC CTCGTTCCGT CTGGCCCGCA GCTCCACCCA ACCGCTGCAG 
TCGCCGCGAG TTGTCCACCT GGCCGGTCAG GCTACTGGTG GCTCCGCTTT GGCACGCCAG 
AACTCGGTGA ACGACAGCAT CAATCTCAGC GAGCAGCTAA CCCTATCCAT CGGCACGGAC 
AGCGAGCACA TCTTTGAGAT GTCCGCTTTG GAGGAGGACT CGGCTATTCA GTCGCTGAGC 
GACGAGACAG CCGCTTCCGC ATCTGCCAAC TCAACATCCA CGACCACTGC AACCACCATG 
ACACCGCCCA GTGGCCTGAC GCGGAGCAAC AGCGTTCGGG CCAGGGCCAA TATGTTCCAG 
CAATTGCAGG AGCAGTCGCG CAACCAACGG GCAACGGGAA CCACCAACTC CCGTCAATCT 
CCACCAGCGT CGTCGGCGGA GGTGTCTTCA AGTGCAGCCG ACAATTCCAT GCACAGTGAC 
GAGCGGTCCA CGCCAAATGC AACGATAGAT GCGGAATTCG GAAAACCCAG CACCGAACCA 
CTTAAGAGCA TTCTTAAGAC GGGAATGCCA GTGATGGGTA TGGGACGCGG TTTAATGCCT 
GTGGATCTCA ATGCCGAGCT GAAGAACCGG CTCAAACGCT CCACACATGC CACTGTTTCC 
AATCTCCGCA AATCTGCCAC CTCGGCAGAT GCCACTAGAG CAGCTAGTGA TGAGGTGGAC 
AATCCGCAGA GGAATTTAGC CCAGATCCTG AGGAATGTTT CCAAGGAGAA CTCCACGCCG 
AAGCACGATG ATGCCGTGAG CCTAGTGCGG AATCTCTCTA CTTTGGGACA ACCTCGATCG 
CTGGCCCGTG ACGATGCAGC CGGGAATTGT GCCTCGGCAT CCGAAGGCGA GAGTTCGGGG 
GGCCGCGAGA TCGAGGCCAT AATCAAGAAC AGCGCTGTGG CCAGACGTCG CCGGCAGCAA 
CAGCAGCAGT CGCAGCAGCA GAATCAGCCA GATGGCAATC TCGTTGCGAA AAGCAAAAGT 
CACTCGGGCA TATCAGCACC CATCGGCCTT GGCCCCCAGC TACCACCCCT TCCCCTCGGC 
GGCGGTTTTG TTAAGTTGCG TCATGTAGCC AAGGAGGAGG ATACCAGTAG CAGCAGCAAC 
ACATCCTCCG CCAAGGAATC GCCAGATAAG AAGGATCTTC GCCAGGAGCA GGCAGAAGCT 
TCGGGAGACT CATCGCACCC AACGGCTCTG CCACAGTTTC TTCAAGGCGT CCAACGATCA 
TCAACCGAGG TCATGGGCCC CGATCAGCGG TCTTTGGTGA TTTCGAAGAC CGGCTCCATT 
GCGGAGCGTT TGGCGGCACT CCACAAAAGT GGCGAGGACG ACTGGAAGAA GCGCATCTCG 
AAGCGCGACG AAGTCGACGA TGTGCACCGT GAAAACTTCG TAAACGAATC GCTATCTTTG 
GCCCACTCGC TGTCGGACAA ACCACTGCCC CCATCACCTT TGATACCCAC CAGCTTGGAG 
GGCGGCAAGG TCTCCGATCG TTTGAGCAAA CTCAAGTGCA ACTCGGAGAA CTGGAAGCAG 
CGCATAGAGC AAACCGATGC CAAGAAATTC ACAGTCGCCG GTCGACTGCA AAAGAAGGCC 
CAATCCCCCG TGGAGCTTCA ATTCGAGCGT TCTGCCAATG ATGCGGCCAA AAAGTGTCCC 
ATGCTGGAGG TGCGCAGCGC CAATCAGCCG CAGCTTGGGC TGGCCAAGAG CCCCTCCATG 
ATGGTCACCT CTAGCACCAG CACCAATAGC ACCAGCAAGT CCGCACTCCG CAGCCTGAGC 
GTCAATCCCG AGGAGGAGGG ACCCACCAGC CTGAGTCGCT CCAACAGCAG CAGTTCGGAG 
TCCGATGGAG AGCGCAAATC CCCCCAGAAT GCCAAGCAGA ACAAGGAGCT GGCCAACAAC 
AAGAGTGCTG CAGCACCCAC CAATGTGGGA GCCAGGATTC AGGTGCCCCG GCTGGACGAC 
AAGGAAACTT TCGAGAACTT CTTCTCCTCC AAGCCGAAGA AGGAGGAGAA CGTTCAGCTG 
GAGATCAGCG CTTTCGATGA CATCAAGCCC ACTGAGCGCT TGGTAAGCAA GCGACACGTA 
CAGGGTCCCA AAGGTCGACG GGCTGCACGC AATCCTCTCA AGAGTTTAGC TGCTCGTGAG 
GACATTAGCT CCGAGTACAC CGAAATGAGG TCGGGCATAG CCGAGCGAGA ATTACGGCGC 
CTAAAGTTGG AGTCATATGG TCATAATGCC AACTTGGCTG CAGAGGCCAT AGCCGGATTA 
GCCTCCGTCG AGGACTTCAA GTCGGTGGCA CTGCGCTCCT CGTCGATTCC ACTGCAGCAA 
ATGTGGCTAC CCCACAAGCC AGTGATGTTA CTCCATGTTA AGGGGCGCAC ACATGTCCAA 
ACCCGACTGG TGGAACCCGT GCATACCTCC CTGAACCGTG GTGATTGCTT CATCCTGGTG 
GCCGGATCGC AGCTTTTCCG CTACGTTGGA TCCTTTGCAA ACGTAATCGA GATCTCCCGG 
AGCAAGAAGA TCTGCGCTGC CATAGTGGAA AACAAGGATC TCGGTTGCAC CGCCACCCAG 
GAGGTGATCC TCACGGATGG CAAGTATTTG AAGGAGCGCC AGTGGCGTCA GTTCTGGCAG 
CTTTTGGGCA AAAAAGAGGA TGATCAATCG GATATAGCTG AATGCGGTCA TGCCGATGAA 
GACGACGTGT TCGAGAGCAG CCTGATCGAG ACTAACAAGA TCTACGAGTA CCAGGATGAG 
GCGCTCATTC CGTTGGAGAA ATTCTGGGGC TGCATCCCCA AGGTGGACAT GTTGGACACG 
CGTAAAGTTT TGGTCTTTGA CTTTGGCAGC GAGCTGTATG TGTGGAATGG CAAGAATGCC 
CCTTCCGATG CCAAGCGAGC TGCCATGAGA TTGGCCCAGG AACACTTTGG TGCTGAGAAT 
TCGGCGGACT ATGCCGACTG TTACTTGAAT CCCCTGAACT ATGTCGCCAT AGTGGGTCGT 
CGTGAGAACA CCAAGTATGC CAAGAGGTGC GCACAGCGTC CAGAATGGTG TGTTCTCGGC 
AAGATCACCC AGAACATGGA GACGGTGCTG TTCAAGGAGA AGTTCAGTGA TTGGCCAGAG 
ATAGAACGCG AGGATCTGGA GAAGGATTAC CTATCAAACG GAGTGCATAT GGTGCGTGCT 
TTAAACGGCA CCGCTTTGCA TAAGGGAGAA CCCTACCAGG AACCAAATCT GGTGCTCGAG 
CAGGCCAACT TGGGGCGTGG CAATTTCTAC TACGACACGG ACACCATGCG GCACTTCGAT 
GTGATTACCA AGTCAACGGA CAAATGGCAG ATCCACGAGT TCAACTTTGA TGCGGCGAAT 
AATCGCGATG ACTACGGTCA CTTCTACTCG GCGGAGAGCT ATATAGTGCG CTGGATCTAC 
CAGATTTCGG TCACAGTTCG CGAACTCAGT GGCAAGGTGT CGAACAGAAG CACAGTGGGT 
CGAGACAGGT GTGTCTATTT CACCTGGCAA GGACAGGACT CAAGTGCCAA CGAGAAGGGT 
GCAGCTGCTT TGCTGACCGT AGAGTTGGAC AAGGAGAAAG GCGCACAGAT GCGGGTGTCG 
CAAGGAGATG AGTGCACAGC CTTCGTCCGC CTGTTCCGGC AAATGTGGCA ACATCGTGGA 
CGCAAGGAGC AATGTCTGGA GAGGCACAGC GAGTGGAGGC TCTACCAACT GCAGGGCAAC 
GTCAGCGAGG AGACCATCAT CAAGGAAGTC GTCTGCCAGT CCAGCCATTT GCGATCCCGC 
AGCTCCATGC TGTTAATTCA CGGCAGTCAG GGAAATGTGA TTGTCTGGCA TGGATCGAAG 
AGTGCTCCTC ATACGCGTAA TGTGGCTGTA GGAGTTGCTC AGGATCTGAT CAAGACGGTG 
CCTAAAGATC TCTTCAGCGC CGAAACTGCC AACCTAACTG AGGTGGAGGA GGGTTCCGAC 
GACGATCAGT GCAAGAAGGC CCTGGGATTG AAGGATGAAA GAAATGAATA TGGCAGCCTG 
CTTAAGTCCA CCAAGTCGTT CGATTACCAA ATAAGGATCT TCAACTTCTC CAGCACTCAG 
GGTGTTTTCA AAGCCCTCGA ACTGAGCGAT CCGCTGCGCT GCCAGGATCT GCATAGTCCG 
TATCCCTTCA GCCAGTCGCA GCTGTACAAT GCAAGGCAGC CGACTATCTT TTTGCTGGAC 
GACGGCGATG AGTTGTGGCT CTGGATGGGA TGGTGGCCTC TGGAAGATGT GAAGATAAAC 
ACCGATGAGC GAAGCAGTCC CACCAACGAA AATCGCGCTG GAGTAAATCG CTGGATCTCA 
GAGAGACGTG CTGCCCTCGA GACAGCCGTT GACTATTGGC GTGCCAAGCA CGGCGAGAAC 
GAGAATGAAC CCTTCCATGG CATCAAAGGC CAGGTTGTGT GGGCAGGCCT GGAGCCACTG 
GCCTTTAAGG CGCTGTTTCC CGACTGGACA GAACGTCCGG ATGTAAGAGA AATCAACGAA 
GAAGATGGGC GAACTGATGC TTCCATATCG ATCGCCACAA TGCTGGCACA AATGACGCAG 
ACCGAGTATC CCCTGGAAGT CCTGAAAGCC CGACCGCTGC CCGAAGGTGT GGAGCCCACC 
CGCCTGGAGG TCTACCTTAA TGCGGAGGAC TTCCAGGCGG CACTGGGATG CAGCCGGGCG 
GAATTCGAGC AGCTGCCCAT CTGGAAACAG ACCAAGCTGA AGAAGGAGCG AGGGCTGTTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Svil ( NM_001299985.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286914.1 Drosophila melanogaster supervillin, transcript variant V (Svil), mRNA.

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CloneID	OFa21978
Clone ID Related Accession (Same CDS sequence)	NM_001299985.1
Accession Version	NM_001299985.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	supervillin, isoform V
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCCATG ACCAGCAGCG AGATCCTCCA GTTGAAGTTC CTAAGGCTCT AAACGTCGAG 
CCACCGCAGC CAGAAGACAT CGTCCTGGGC TCCTGCCCCA CTGCATCTGC CCACTCAGAG 
AGCCAAAGTC GAGGAAAAGC TGGCAAATGC AGTATTAATT GCACCTACAG CCACAGCCAC 
AGCACCAACA ACAACTGCAA CGCGCGACAA CGCAACAGCA GCAACATCAG CAGCAACAAC 
ACCATCAGCA GCAACAGCAG CAGCAGCAAA AGCACTTGCA ACAACAATAG CAAACAGCAA 
CACGCCACGC CCATGCCGCC CCTAGCTAAT GCCAACAAAG CCCCGTTAAT TGTGCTTAAG 
ACAGCCGCAT CTGCAGCAGC ATTGCCGTCC ATTGCCGCAG CAGCAACATC CAGCGGCACG 
GCAGCAACAT CTGGTGGTGT TGGTTCGCCC ATCAAGCGTC GCAAGAATCC GCTGTCGCCG 
CTGCTCCTCG GCAGCCAGTG CCAGTCCACC ATTGCGCCTC CGCAGGCCAT TGCCAGTCCG 
CTCCTGAGCG GCGGCAGCTG CTCCTCCTCG ACCACCTCGC CCCAATCCTC GCCAGCGGTT 
CGCACCACCA AGGCGAGTCG TCTGCGCGCC GCCGCGCTTG ATAAAAAGAA GGAAGAGGAG 
CAACGCAATC GCTACAGTTC GCCCGTTTCA CCAAAAGCCT CCAGCAACGG CGGAGTCCAA 
GCACAACGTC GTCTTAGCAA CTCGGAGCTA TCGCCCAAGT CGCCAGGTGC CAGTGGATTC 
ACTTCGGAGC CAGTCGCGTC AGCCACAGCG AACAGAGTCC CGACGAGATC TGCCTCCCAT 
CCTGTGGACA CACCGCCCAC ACAAAGGCGA CAGCCTCTGG TGGCCGTGAG TTCGGGCATC 
TCCATGAAGC AGAGTCTCAG CGAGCTGCAC TTCACGGGTG GAGCAGTCAG TCCGCCCAAG 
GCTACGGCGG AATGCACTCT AAGGATGCGA CCACGTAGCT CCACGATGAG CACCGAGAAC 
ACGGAGAATG GCCGACGTGA CCACTTGGCT AATCTGCAAC TAAGTCCTGT GCAAAAGCAA 
AAGCGAACGC CCGAAGTATC ACCACGCCGT TTCGGAGATC GCGACAAGTC GGCCAAACGG 
AAATCGAATT TGAATCGTTC GTTGACCGAG GAAGCCACCA AGGATGGCGA TGAGGTTGCC 
TCTTCGACCC GCCGGCGCCG ACGTCGTGAA CTGGGAATGG CTCGTCCCCT GGTTCCGGGC 
GATGATGACG CCCTAAGAGA TTTGCTAACG CCGCAGAAGA GTTCCTGCAG CGAGTCAACC 
AACTCCATAT CCGAACCCCA TCTCCAGTTC ATACCAGCCG TTCGTCATCC GGTGGTTCCA 
GTACGCACCA TGACTGCTGT GCTGCCTGCA CCACGACCGC TGCATACTCC TGCGGCCCGC 
AACGTCAGCC AGAAGTTCAA TGAAAGGACC AAAACAGATC TGGCCGAGAT CAAGAAGATT 
GCTGAGCAAT CGGAGGCGCA GGTGCAACAG CCTTCGGTGG ATGATAACCA GAAAATGTCA 
GCCATAATGC GCAGGAAGAG CACGGATGAG GGTGGATCAG CTTTGGGGGC AACAAACCAG 
AATTCACCAC CTGCCCCAAA CCAAATTGTA TCCATACTGA AGAAGAAGGA ACATGGACTT 
GGCGAGTGCA ACTCCAGTGC TTCAAGCAAT CCCTCGCCGG TGACCTTCTC TTCTAGCGTG 
GTGGATCATC CTTTGGCTGG AGCTGGCCGG TGCAAACGGC AGGGAATACT CAAGAAGAGA 
TCGAGTCTAG ATGAATCGCG CTACTACTCC CGATCGCATT CACCCGACGA ACGTAGTATT 
CTCATTAAGT CGGCCAGGAG GAATTCCCTG GAGGAAGCTG GCACTGGATT GAGTCCGGCT 
CAGGCCCATG GAATCCTCAA GCAGAGCAGC TACGACAGCA GCAAGAGCGA CGGCTGCCCT 
TCGGCCACGG AGAGCCAGCC ACACAGTATC CTGAAAAAGA AGGATTCGCT ATCGACACCT 
TCGGACGGAG GATGCCACAA GCATGTCTCT ATATCGCAAG CTGTAACTTT AGCTGCTGCT 
GAACTGGCTG CCCATGATGG AATCACCTTC GATGATGGTG AGGAGCATGA GATACGACCG 
ATCCTAAAGC AGGAGAGCAC CTCCAGCGAG GAGGCTGTGC GTCCGCCAAA GCCCATACTC 
AAAAAGAAAT CCTTTGGCGA GGCGGATGAA CACGAAATAC GTCCTATATT GAAGAGCTCT 
CGTAAAAGCA GTCGAGAGGA GTTCGATTTG AGTGGCTTGG AAAACGAGGT CAACGATTCC 
CTATCGTCAA TCCTAAAGAC AGACTCGCCG TCCAAGCGTC GGTCGCTCGG TTCAGCTTTG 
CATGATTTGG AGGAGTCCAC CACTTCCCCG AGCATGCTCA AGAGGCGCAC CCGTTCGCTG 
GAACGTCAAG ATGTCCAGCC TGGCATGGAT TTGGCAGCTG CACTGGATGC CATCGCCACA 
TCACAGGCTA CTCCCAGCAC CCCGGTGGAC TTCGTGACCA CACCAACAAG TATATCGGTG 
GCGGAGCGGA TCAAGCGCAT GGAGATGCTG TCGACGCCCA CTTCCCGTGC GGCGGGTGGG 
GCCAACAGCA GCTGGGAATC GCCACTGCAG GCATCGTCAG CAGAGCATGG AGCACCCACC 
ACCCCAAGGG TTCTCAAGCC AAGTGTGCTG CGCAGAGATT TGCAGAGGGA GCGGTACAAA 
ACGCAACCCG TGACCAACGA GGAGATGAGC TTCTTCAAAG CCAACTCCGC GCCCTTCGAC 
ATCTCGGCTA CATCGGCCTT CAAGCCCACC AAGCCGCTGG ACATCCGCTT GACCCACGCA 
CCGCATGCAC CGCTGATTTC GCCGATCACC GGACAGCCAC TCAGCCACGT GCCCGCGCCA 
CTGCTGCTAT CCTCGTCGAC GAACTCGGGA GCGGGATCCT CGTTCCGTCT GGCCCGCAGC 
TCCACCCAAC CGCTGCAGTC GCCGCGAGTT GTCCACCTGG CCGGTCAGGC TACTGGTGGC 
TCCGCTTTGG CACGCCAGAA CTCGGTGAAC GACAGCATCA ATCTCAGCGA GCAGCTAACC 
CTATCCATCG GCACGGACAG CGAGCACATC TTTGAGATGT CCGCTTTGGA GGAGGACTCG 
GCTATTCAGT CGCTGAGCGA CGAGACAGCC GCTTCCGCAT CTGCCAACTC AACATCCACG 
ACCACTGCAA CCACCATGAC ACCGCCCAGT GGCCTGACGC GGAGCAACAG CGTTCGGGCC 
AGGGCCAATA TGTTCCAGCA ATTGCAGGAG CAGTCGCGCA ACCAACGGGC AACGGGAACC 
ACCAACTCCC GTCAATCTCC ACCAGCGTCG TCGGCGGAGG TGTCTTCAAG TGCAGCCGAC 
AATTCCATGC ACAGTGACGA GCGGTCCACG CCAAATGCAA CGATAGATGC GGAATTCGAA 
CCCTCATCCC TGTCGCTGGC TGAGCGACTG GCCTTCTTCA GCAATCTGTG CGAGAGCGGC 
GGAGGAGGTG GGAGTACCAG TGCCGGTGGA AGGTACCGCA GTCGCACCAG CAGCTATTCA 
AGGAGTCCGC CCGGCGATCG CACCACTCCC AGGAGCAGCA CCACCGCCAG CAGTGTCAGT 
ATGACGCCCA CGCCCATGGA TTACTCTCCG ATTCCCCACG ACAAGGAGCC ATCTAGTCTC 
ACGCTGGAGA GCATCATCGA GCCCGAGCAG GAGGAGGTCA AGGAGCAGGT GGACCAGGAT 
GAGGTCAAGT TGAGGAAGAA GGAGCCAGTC CAGAGGGAAT CTTCCCAGCA CAATGGCTTC 
CACGTGCTGA CCAGGTCCGC CACCGAACCG CCGTCGTCCA CCTCGCCCCT GCGCATCAGC 
ATACGGACTG TGGGAAAGCT AGTCCTGCCC GATACCTTCC GCGGCGATCG CAACAATAAC 
AGCAGTAGTA GAAGTGGGAG CAACAGCTGG AGCAACACTT GCATGGTCAA GCTGCAGAGC 
TTGGAGCCCG TTAGCCTGTC CGTTCATTCG CGCACCATTG GTAAGGTTAA GTCGCCATTC 
ATTGAAAAGC AGCAGGAGAA ACCACACACC GAGAAGCTAG CGAACGGAAC CAGCGACAGT 
GGCTCCGATT CGGGCAAGGA GAACTGCGCC TCCAACGAGC AGGAACATCT GCAGCACCAG 
CAGTCGCAGG ACGAGGATCG AGCTCCGCCC AGGGTGTCCG AGATGCGGAG GAAAATCAGC 
CGACTGGTGC AACAACAGCA GCCGCAACCA CAGCCCGTCA ATCGGCGGCA CACCACCGAG 
ATCACCTCCG TGACCGTTGG AACGCGTTCG CCCAGTGTGG ACGATCGCTT TGCCAAGTAC 
TTTGGTGTTA AGGAGAGCTG CAACAGCACG ACCACCGTGC ATAAAACGCA ATCGCTGCCA 
CCCAGCCAAG TTGAGGCCAC CAATGCGCAC GTGAAACTAC CGCAGATCAC TACCGTGGTT 
TCCAAACGGC GCGAGCTGCT CAAGAGGACG CGACCCCTGT CCATGGATCA CTACTCCAGC 
GGCTGCACCA GTCCCACAGC CATGCCAAGT CCCAAGAGAG CACATTCGCC CGTCGGCCGG 
CGGAAGGCCG CCATTTCCAG CATCGAGGAC ATTGAGGTCA CGCCGGATGA ACTCCGGGCC 
GCTGGCAGGA ACTTCAAGCT GTTGTACGGC GAGTTATTGG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286914.1
CDS	395..5119
Translation	MTHDQQRDPPVEVPKALNVEPPQPEDIVLGSCPTASAHSESQSRGKAGKCSINCTYSHSHSTNNNCNARQRNSSNISSNNTISSNSSSSKSTCNNNSKQQHATPMPPLANANKAPLIVLKTAASAAALPSIAAAATSSGTAATSGGVGSPIKRRKNPLSPLLLGSQCQSTIAPPQAIASPLLSGGSCSSSTTSPQSSPAVRTTKASRLRAAALDKKKEEEQRNRYSSPVSPKASSNGGVQAQRRLSNSELSPKSPGASGFTSEPVASATANRVPTRSASHPVDTPPTQRRQPLVAVSSGISMKQSLSELHFTGGAVSPPKATAECTLRMRPRSSTMSTENTENGRRDHLANLQLSPVQKQKRTPEVSPRRFGDRDKSAKRKSNLNRSLTEEATKDGDEVASSTRRRRRRELGMARPLVPGDDDALRDLLTPQKSSCSESTNSISEPHLQFIPAVRHPVVPVRTMTAVLPAPRPLHTPAARNVSQKFNERTKTDLAEIKKIAEQSEAQVQQPSVDDNQKMSAIMRRKSTDEGGSALGATNQNSPPAPNQIVSILKKKEHGLGECNSSASSNPSPVTFSSSVVDHPLAGAGRCKRQGILKKRSSLDESRYYSRSHSPDERSILIKSARRNSLEEAGTGLSPAQAHGILKQSSYDSSKSDGCPSATESQPHSILKKKDSLSTPSDGGCHKHVSISQAVTLAAAELAAHDGITFDDGEEHEIRPILKQESTSSEEAVRPPKPILKKKSFGEADEHEIRPILKSSRKSSREEFDLSGLENEVNDSLSSILKTDSPSKRRSLGSALHDLEESTTSPSMLKRRTRSLERQDVQPGMDLAAALDAIATSQATPSTPVDFVTTPTSISVAERIKRMEMLSTPTSRAAGGANSSWESPLQASSAEHGAPTTPRVLKPSVLRRDLQRERYKTQPVTNEEMSFFKANSAPFDISATSAFKPTKPLDIRLTHAPHAPLISPITGQPLSHVPAPLLLSSSTNSGAGSSFRLARSSTQPLQSPRVVHLAGQATGGSALARQNSVNDSINLSEQLTLSIGTDSEHIFEMSALEEDSAIQSLSDETAASASANSTSTTTATTMTPPSGLTRSNSVRARANMFQQLQEQSRNQRATGTTNSRQSPPASSAEVSSSAADNSMHSDERSTPNATIDAEFEPSSLSLAERLAFFSNLCESGGGGGSTSAGGRYRSRTSSYSRSPPGDRTTPRSSTTASSVSMTPTPMDYSPIPHDKEPSSLTLESIIEPEQEEVKEQVDQDEVKLRKKEPVQRESSQHNGFHVLTRSATEPPSSTSPLRISIRTVGKLVLPDTFRGDRNNNSSSRSGSNSWSNTCMVKLQSLEPVSLSVHSRTIGKVKSPFIEKQQEKPHTEKLANGTSDSGSDSGKENCASNEQEHLQHQQSQDEDRAPPRVSEMRRKISRLVQQQQPQPQPVNRRHTTEITSVTVGTRSPSVDDRFAKYFGVKESCNSTTTVHKTQSLPPSQVEATNAHVKLPQITTVVSKRRELLKRTRPLSMDHYSSGCTSPTAMPSPKRAHSPVGRRKAAISSIEDIEVTPDELRAAGRNFKLLYGELLG

Target ORF information:

RefSeq Version	NM_001299985.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster supervillin, transcript variant V (Svil), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299985.1

ORF Insert Sequence:

ATGACCCATG ACCAGCAGCG AGATCCTCCA GTTGAAGTTC CTAAGGCTCT AAACGTCGAG 
CCACCGCAGC CAGAAGACAT CGTCCTGGGC TCCTGCCCCA CTGCATCTGC CCACTCAGAG 
AGCCAAAGTC GAGGAAAAGC TGGCAAATGC AGTATTAATT GCACCTACAG CCACAGCCAC 
AGCACCAACA ACAACTGCAA CGCGCGACAA CGCAACAGCA GCAACATCAG CAGCAACAAC 
ACCATCAGCA GCAACAGCAG CAGCAGCAAA AGCACTTGCA ACAACAATAG CAAACAGCAA 
CACGCCACGC CCATGCCGCC CCTAGCTAAT GCCAACAAAG CCCCGTTAAT TGTGCTTAAG 
ACAGCCGCAT CTGCAGCAGC ATTGCCGTCC ATTGCCGCAG CAGCAACATC CAGCGGCACG 
GCAGCAACAT CTGGTGGTGT TGGTTCGCCC ATCAAGCGTC GCAAGAATCC GCTGTCGCCG 
CTGCTCCTCG GCAGCCAGTG CCAGTCCACC ATTGCGCCTC CGCAGGCCAT TGCCAGTCCG 
CTCCTGAGCG GCGGCAGCTG CTCCTCCTCG ACCACCTCGC CCCAATCCTC GCCAGCGGTT 
CGCACCACCA AGGCGAGTCG TCTGCGCGCC GCCGCGCTTG ATAAAAAGAA GGAAGAGGAG 
CAACGCAATC GCTACAGTTC GCCCGTTTCA CCAAAAGCCT CCAGCAACGG CGGAGTCCAA 
GCACAACGTC GTCTTAGCAA CTCGGAGCTA TCGCCCAAGT CGCCAGGTGC CAGTGGATTC 
ACTTCGGAGC CAGTCGCGTC AGCCACAGCG AACAGAGTCC CGACGAGATC TGCCTCCCAT 
CCTGTGGACA CACCGCCCAC ACAAAGGCGA CAGCCTCTGG TGGCCGTGAG TTCGGGCATC 
TCCATGAAGC AGAGTCTCAG CGAGCTGCAC TTCACGGGTG GAGCAGTCAG TCCGCCCAAG 
GCTACGGCGG AATGCACTCT AAGGATGCGA CCACGTAGCT CCACGATGAG CACCGAGAAC 
ACGGAGAATG GCCGACGTGA CCACTTGGCT AATCTGCAAC TAAGTCCTGT GCAAAAGCAA 
AAGCGAACGC CCGAAGTATC ACCACGCCGT TTCGGAGATC GCGACAAGTC GGCCAAACGG 
AAATCGAATT TGAATCGTTC GTTGACCGAG GAAGCCACCA AGGATGGCGA TGAGGTTGCC 
TCTTCGACCC GCCGGCGCCG ACGTCGTGAA CTGGGAATGG CTCGTCCCCT GGTTCCGGGC 
GATGATGACG CCCTAAGAGA TTTGCTAACG CCGCAGAAGA GTTCCTGCAG CGAGTCAACC 
AACTCCATAT CCGAACCCCA TCTCCAGTTC ATACCAGCCG TTCGTCATCC GGTGGTTCCA 
GTACGCACCA TGACTGCTGT GCTGCCTGCA CCACGACCGC TGCATACTCC TGCGGCCCGC 
AACGTCAGCC AGAAGTTCAA TGAAAGGACC AAAACAGATC TGGCCGAGAT CAAGAAGATT 
GCTGAGCAAT CGGAGGCGCA GGTGCAACAG CCTTCGGTGG ATGATAACCA GAAAATGTCA 
GCCATAATGC GCAGGAAGAG CACGGATGAG GGTGGATCAG CTTTGGGGGC AACAAACCAG 
AATTCACCAC CTGCCCCAAA CCAAATTGTA TCCATACTGA AGAAGAAGGA ACATGGACTT 
GGCGAGTGCA ACTCCAGTGC TTCAAGCAAT CCCTCGCCGG TGACCTTCTC TTCTAGCGTG 
GTGGATCATC CTTTGGCTGG AGCTGGCCGG TGCAAACGGC AGGGAATACT CAAGAAGAGA 
TCGAGTCTAG ATGAATCGCG CTACTACTCC CGATCGCATT CACCCGACGA ACGTAGTATT 
CTCATTAAGT CGGCCAGGAG GAATTCCCTG GAGGAAGCTG GCACTGGATT GAGTCCGGCT 
CAGGCCCATG GAATCCTCAA GCAGAGCAGC TACGACAGCA GCAAGAGCGA CGGCTGCCCT 
TCGGCCACGG AGAGCCAGCC ACACAGTATC CTGAAAAAGA AGGATTCGCT ATCGACACCT 
TCGGACGGAG GATGCCACAA GCATGTCTCT ATATCGCAAG CTGTAACTTT AGCTGCTGCT 
GAACTGGCTG CCCATGATGG AATCACCTTC GATGATGGTG AGGAGCATGA GATACGACCG 
ATCCTAAAGC AGGAGAGCAC CTCCAGCGAG GAGGCTGTGC GTCCGCCAAA GCCCATACTC 
AAAAAGAAAT CCTTTGGCGA GGCGGATGAA CACGAAATAC GTCCTATATT GAAGAGCTCT 
CGTAAAAGCA GTCGAGAGGA GTTCGATTTG AGTGGCTTGG AAAACGAGGT CAACGATTCC 
CTATCGTCAA TCCTAAAGAC AGACTCGCCG TCCAAGCGTC GGTCGCTCGG TTCAGCTTTG 
CATGATTTGG AGGAGTCCAC CACTTCCCCG AGCATGCTCA AGAGGCGCAC CCGTTCGCTG 
GAACGTCAAG ATGTCCAGCC TGGCATGGAT TTGGCAGCTG CACTGGATGC CATCGCCACA 
TCACAGGCTA CTCCCAGCAC CCCGGTGGAC TTCGTGACCA CACCAACAAG TATATCGGTG 
GCGGAGCGGA TCAAGCGCAT GGAGATGCTG TCGACGCCCA CTTCCCGTGC GGCGGGTGGG 
GCCAACAGCA GCTGGGAATC GCCACTGCAG GCATCGTCAG CAGAGCATGG AGCACCCACC 
ACCCCAAGGG TTCTCAAGCC AAGTGTGCTG CGCAGAGATT TGCAGAGGGA GCGGTACAAA 
ACGCAACCCG TGACCAACGA GGAGATGAGC TTCTTCAAAG CCAACTCCGC GCCCTTCGAC 
ATCTCGGCTA CATCGGCCTT CAAGCCCACC AAGCCGCTGG ACATCCGCTT GACCCACGCA 
CCGCATGCAC CGCTGATTTC GCCGATCACC GGACAGCCAC TCAGCCACGT GCCCGCGCCA 
CTGCTGCTAT CCTCGTCGAC GAACTCGGGA GCGGGATCCT CGTTCCGTCT GGCCCGCAGC 
TCCACCCAAC CGCTGCAGTC GCCGCGAGTT GTCCACCTGG CCGGTCAGGC TACTGGTGGC 
TCCGCTTTGG CACGCCAGAA CTCGGTGAAC GACAGCATCA ATCTCAGCGA GCAGCTAACC 
CTATCCATCG GCACGGACAG CGAGCACATC TTTGAGATGT CCGCTTTGGA GGAGGACTCG 
GCTATTCAGT CGCTGAGCGA CGAGACAGCC GCTTCCGCAT CTGCCAACTC AACATCCACG 
ACCACTGCAA CCACCATGAC ACCGCCCAGT GGCCTGACGC GGAGCAACAG CGTTCGGGCC 
AGGGCCAATA TGTTCCAGCA ATTGCAGGAG CAGTCGCGCA ACCAACGGGC AACGGGAACC 
ACCAACTCCC GTCAATCTCC ACCAGCGTCG TCGGCGGAGG TGTCTTCAAG TGCAGCCGAC 
AATTCCATGC ACAGTGACGA GCGGTCCACG CCAAATGCAA CGATAGATGC GGAATTCGAA 
CCCTCATCCC TGTCGCTGGC TGAGCGACTG GCCTTCTTCA GCAATCTGTG CGAGAGCGGC 
GGAGGAGGTG GGAGTACCAG TGCCGGTGGA AGGTACCGCA GTCGCACCAG CAGCTATTCA 
AGGAGTCCGC CCGGCGATCG CACCACTCCC AGGAGCAGCA CCACCGCCAG CAGTGTCAGT 
ATGACGCCCA CGCCCATGGA TTACTCTCCG ATTCCCCACG ACAAGGAGCC ATCTAGTCTC 
ACGCTGGAGA GCATCATCGA GCCCGAGCAG GAGGAGGTCA AGGAGCAGGT GGACCAGGAT 
GAGGTCAAGT TGAGGAAGAA GGAGCCAGTC CAGAGGGAAT CTTCCCAGCA CAATGGCTTC 
CACGTGCTGA CCAGGTCCGC CACCGAACCG CCGTCGTCCA CCTCGCCCCT GCGCATCAGC 
ATACGGACTG TGGGAAAGCT AGTCCTGCCC GATACCTTCC GCGGCGATCG CAACAATAAC 
AGCAGTAGTA GAAGTGGGAG CAACAGCTGG AGCAACACTT GCATGGTCAA GCTGCAGAGC 
TTGGAGCCCG TTAGCCTGTC CGTTCATTCG CGCACCATTG GTAAGGTTAA GTCGCCATTC 
ATTGAAAAGC AGCAGGAGAA ACCACACACC GAGAAGCTAG CGAACGGAAC CAGCGACAGT 
GGCTCCGATT CGGGCAAGGA GAACTGCGCC TCCAACGAGC AGGAACATCT GCAGCACCAG 
CAGTCGCAGG ACGAGGATCG AGCTCCGCCC AGGGTGTCCG AGATGCGGAG GAAAATCAGC 
CGACTGGTGC AACAACAGCA GCCGCAACCA CAGCCCGTCA ATCGGCGGCA CACCACCGAG 
ATCACCTCCG TGACCGTTGG AACGCGTTCG CCCAGTGTGG ACGATCGCTT TGCCAAGTAC 
TTTGGTGTTA AGGAGAGCTG CAACAGCACG ACCACCGTGC ATAAAACGCA ATCGCTGCCA 
CCCAGCCAAG TTGAGGCCAC CAATGCGCAC GTGAAACTAC CGCAGATCAC TACCGTGGTT 
TCCAAACGGC GCGAGCTGCT CAAGAGGACG CGACCCCTGT CCATGGATCA CTACTCCAGC 
GGCTGCACCA GTCCCACAGC CATGCCAAGT CCCAAGAGAG CACATTCGCC CGTCGGCCGG 
CGGAAGGCCG CCATTTCCAG CATCGAGGAC ATTGAGGTCA CGCCGGATGA ACTCCGGGCC 
GCTGGCAGGA ACTTCAAGCT GTTGTACGGC GAGTTATTGG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Svil
Entrez Gene ID	38306
Full Name	CG45186 gene product from transcript CG45186-RT
Synonyms	CG1141,CG12790,CG13797,CG13798,CG13799,CG13800,CG16763,CG16764,CG32306,CG33231,CG33232,CG42669,Dmel\CG45186,Dmel_CG13800,Dmel_CG32306,Dmel_CG33231,Dmel_CG33232,Dmel_CG42669,c62E-11
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 62D7-62E1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Svil cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

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