Rap1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rap1
Entrez Gene ID	38244
Full Name	Rap1 GTPase
Synonyms	BEST:GH18528,C-ras3,CG1956,D-Rap1,DRap,DRap1,Dmel\CG1956,Dras3,Dras62B,E(faf),EC3-7,FCG-C,P08645,R,RAP1,Rap,Ras3,RasIII,l(3)62Bf,l(3)R,rap1,ras 3,ras3
General protein information	Preferred Names Rap1 GTPase Names CG1956 gene product from transcript CG1956-RA CG1956-PA CG1956-PB CG1956-PC Rap1-PA Rap1-PB Rap1-PC enhancer of faf roughened
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 62B7-62B7\|3-1.4 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_057509.5	NP_476857.1	Rap1 GTPase, isoform A
NM_001202094.2	NP_001189023.1	Rap1 GTPase, isoform B
NM_001274366.1	NP_001261295.1	Rap1 GTPase, isoform C

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RAP1A	XP_524793.1
Mus musculus (house mouse)	Rap1a	NP_663516.1
Homo sapiens (human)	RAP1A	NP_001010935.1
Danio rerio (zebrafish)	rap1ab	NP_001230108.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001874	XP_003435963.1
Rattus norvegicus (Norway rat)	Rap1a	NP_001005765.1
Drosophila melanogaster (fruit fly)	R	NP_001189023.1
Eremothecium gossypii	AGOS_AFR464W	NP_986011.1
Magnaporthe oryzae (rice blast fungus)	MGG_02727	XP_003721031.1
	RAP1A	XP_001106027.1
Bos taurus (cattle)	RAP1A	NP_776873.1
Gallus gallus (chicken)	RAP1A	XP_417994.1
Neurospora crassa	NCU02167	XP_964526.2
Xenopus tropicalis (tropical clawed frog)	rap1a	NP_001096357.1
Canis lupus familiaris (dog)	RAP1A	XP_863066.1
Danio rerio (zebrafish)	rap1aa	NP_001002152.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Gain-of-function studies using dominant-negative and constitutively active forms of Rap1 indicate that Rap1 contributes to axonal growth and guidance.
Title: The axon guidance function of Rap1 small GTPase is independent of PlexA RasGAP activity in Drosophila.

Rap1 is activated to its GTP-bound form in response to Hh ligand, and that the GTPase exchange factor C3G likely contributes to this activation.
Title: The small GTPase Rap1 is a modulator of Hedgehog signaling.

We show that Rap1 activity induces and/or maintains the formation of the apical domain of developing photoreceptor cells and that this event correlates with the apical accumulation and activity of SEV in presumptive R7 cells.
Title: Apical accumulation of the Sevenless receptor tyrosine kinase during Drosophila eye development is promoted by the small GTPase Rap1.

According to Bridges' map, all three bands (Dras1, Dras2, Dras3) were mapped to regions 85D, 64B, and 62B of chromosome 3. Among them, only Drasl has a common origin with ras genes of mammals.
Title: [Universal intracellular transducer RAS and its role in the development of Drosophila].

Increased Rap1 activity restricts epithelial invagination in an alpha-catenin-dependent manner.
Title: Distinct Rap1 activity states control the extent of epithelial invagination via ?-catenin.

The following Rap1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rap1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa17240	NM_001274366.1 Latest version!	Drosophila melanogaster Rap1 GTPase (Rap1), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa17240	NM_057509.5 Latest version!	Drosophila melanogaster Rap1 GTPase (Rap1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa17240	NM_001202094.2 Latest version!	Drosophila melanogaster Rap1 GTPase (Rap1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rap1 ( NM_057509.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476857.1 Drosophila melanogaster Rap1 GTPase (Rap1), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa17240
Clone ID Related Accession (Same CDS sequence)	NM_057509.5 , NM_001202094.2 , NM_001274366.1
Accession Version	NM_057509.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rap1 GTPase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_057509.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGTGAGT ACAAAATCGT GGTCCTTGGA AGCGGCGGCG TGGGCAAATC CGCGCTGACA 
GTCCAGTTTG TCCAGTGCAT CTTCGTGGAG AAGTACGATC CCACCATCGA GGACAGTTAC 
AGGAAGCAGG TGGAGGTGGA CGGACAGCAG TGCATGCTGG AGATCCTGGA CACGGCGGGT 
ACGGAGCAGT TCACGGCCAT GCGGGATTTG TACATGAAGA ACGGCCAGGG ATTCGTGCTG 
GTCTACTCGA TCACGGCGCA ATCGACGTTT AACGATCTGC AGGACCTGCG CGAGCAGATA 
CTCCGGGTGA AGGACACGGA CGATGTGCCC ATGGTGCTCG TGGGCAACAA GTGCGACCTC 
GAAGAGGAGC GCGTCGTCGG CAAGGAGCTG GGCAAGAACC TGGCCACCCA GTTCAACTGC 
GCCTTCATGG AGACCTCAGC CAAAGCCAAA GTGAATGTGA ACGATATTTT CTACGACCTG 
GTCCGGCAGA TCAACAAGAA GTCGCCCGAG AAGAAACAGA AGAAGCCGAA AAAGTCCCTA 
TGTGTTCTGC TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476857.1
CDS	645..1199
Translation	MREYKIVVLGSGGVGKSALTVQFVQCIFVEKYDPTIEDSYRKQVEVDGQQCMLEILDTAGTEQFTAMRDLYMKNGQGFVLVYSITAQSTFNDLQDLREQILRVKDTDDVPMVLVGNKCDLEEERVVGKELGKNLATQFNCAFMETSAKAKVNVNDIFYDLVRQINKKSPEKKQKKPKKSLCVLL

Target ORF information:

RefSeq Version	NM_057509.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rap1 GTPase (Rap1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057509.5

ORF Insert Sequence:

ATGCGTGAGT ACAAAATCGT GGTCCTTGGA AGCGGCGGCG TGGGCAAATC CGCGCTGACA 
GTCCAGTTTG TCCAGTGCAT CTTCGTGGAG AAGTACGATC CCACCATCGA GGACAGTTAC 
AGGAAGCAGG TGGAGGTGGA CGGACAGCAG TGCATGCTGG AGATCCTGGA CACGGCGGGT 
ACGGAGCAGT TCACGGCCAT GCGGGATTTG TACATGAAGA ACGGCCAGGG ATTCGTGCTG 
GTCTACTCGA TCACGGCGCA ATCGACGTTT AACGATCTGC AGGACCTGCG CGAGCAGATA 
CTCCGGGTGA AGGACACGGA CGATGTGCCC ATGGTGCTCG TGGGCAACAA GTGCGACCTC 
GAAGAGGAGC GCGTCGTCGG CAAGGAGCTG GGCAAGAACC TGGCCACCCA GTTCAACTGC 
GCCTTCATGG AGACCTCAGC CAAAGCCAAA GTGAATGTGA ACGATATTTT CTACGACCTG 
GTCCGGCAGA TCAACAAGAA GTCGCCCGAG AAGAAACAGA AGAAGCCGAA AAAGTCCCTA 
TGTGTTCTGC TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rap1 ( NM_001202094.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001189023.1 Drosophila melanogaster Rap1 GTPase (Rap1), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa17240
Clone ID Related Accession (Same CDS sequence)	NM_057509.5 , NM_001202094.2 , NM_001274366.1
Accession Version	NM_001202094.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rap1 GTPase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001202094.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGTGAGT ACAAAATCGT GGTCCTTGGA AGCGGCGGCG TGGGCAAATC CGCGCTGACA 
GTCCAGTTTG TCCAGTGCAT CTTCGTGGAG AAGTACGATC CCACCATCGA GGACAGTTAC 
AGGAAGCAGG TGGAGGTGGA CGGACAGCAG TGCATGCTGG AGATCCTGGA CACGGCGGGT 
ACGGAGCAGT TCACGGCCAT GCGGGATTTG TACATGAAGA ACGGCCAGGG ATTCGTGCTG 
GTCTACTCGA TCACGGCGCA ATCGACGTTT AACGATCTGC AGGACCTGCG CGAGCAGATA 
CTCCGGGTGA AGGACACGGA CGATGTGCCC ATGGTGCTCG TGGGCAACAA GTGCGACCTC 
GAAGAGGAGC GCGTCGTCGG CAAGGAGCTG GGCAAGAACC TGGCCACCCA GTTCAACTGC 
GCCTTCATGG AGACCTCAGC CAAAGCCAAA GTGAATGTGA ACGATATTTT CTACGACCTG 
GTCCGGCAGA TCAACAAGAA GTCGCCCGAG AAGAAACAGA AGAAGCCGAA AAAGTCCCTA 
TGTGTTCTGC TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189023.1
CDS	776..1330
Translation	MREYKIVVLGSGGVGKSALTVQFVQCIFVEKYDPTIEDSYRKQVEVDGQQCMLEILDTAGTEQFTAMRDLYMKNGQGFVLVYSITAQSTFNDLQDLREQILRVKDTDDVPMVLVGNKCDLEEERVVGKELGKNLATQFNCAFMETSAKAKVNVNDIFYDLVRQINKKSPEKKQKKPKKSLCVLL

Target ORF information:

RefSeq Version	NM_001202094.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rap1 GTPase (Rap1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202094.2

ORF Insert Sequence:

ATGCGTGAGT ACAAAATCGT GGTCCTTGGA AGCGGCGGCG TGGGCAAATC CGCGCTGACA 
GTCCAGTTTG TCCAGTGCAT CTTCGTGGAG AAGTACGATC CCACCATCGA GGACAGTTAC 
AGGAAGCAGG TGGAGGTGGA CGGACAGCAG TGCATGCTGG AGATCCTGGA CACGGCGGGT 
ACGGAGCAGT TCACGGCCAT GCGGGATTTG TACATGAAGA ACGGCCAGGG ATTCGTGCTG 
GTCTACTCGA TCACGGCGCA ATCGACGTTT AACGATCTGC AGGACCTGCG CGAGCAGATA 
CTCCGGGTGA AGGACACGGA CGATGTGCCC ATGGTGCTCG TGGGCAACAA GTGCGACCTC 
GAAGAGGAGC GCGTCGTCGG CAAGGAGCTG GGCAAGAACC TGGCCACCCA GTTCAACTGC 
GCCTTCATGG AGACCTCAGC CAAAGCCAAA GTGAATGTGA ACGATATTTT CTACGACCTG 
GTCCGGCAGA TCAACAAGAA GTCGCCCGAG AAGAAACAGA AGAAGCCGAA AAAGTCCCTA 
TGTGTTCTGC TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rap1 ( NM_001274366.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261295.1 Drosophila melanogaster Rap1 GTPase (Rap1), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa17240
Clone ID Related Accession (Same CDS sequence)	NM_057509.5 , NM_001202094.2 , NM_001274366.1
Accession Version	NM_001274366.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rap1 GTPase, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGTGAGT ACAAAATCGT GGTCCTTGGA AGCGGCGGCG TGGGCAAATC CGCGCTGACA 
GTCCAGTTTG TCCAGTGCAT CTTCGTGGAG AAGTACGATC CCACCATCGA GGACAGTTAC 
AGGAAGCAGG TGGAGGTGGA CGGACAGCAG TGCATGCTGG AGATCCTGGA CACGGCGGGT 
ACGGAGCAGT TCACGGCCAT GCGGGATTTG TACATGAAGA ACGGCCAGGG ATTCGTGCTG 
GTCTACTCGA TCACGGCGCA ATCGACGTTT AACGATCTGC AGGACCTGCG CGAGCAGATA 
CTCCGGGTGA AGGACACGGA CGATGTGCCC ATGGTGCTCG TGGGCAACAA GTGCGACCTC 
GAAGAGGAGC GCGTCGTCGG CAAGGAGCTG GGCAAGAACC TGGCCACCCA GTTCAACTGC 
GCCTTCATGG AGACCTCAGC CAAAGCCAAA GTGAATGTGA ACGATATTTT CTACGACCTG 
GTCCGGCAGA TCAACAAGAA GTCGCCCGAG AAGAAACAGA AGAAGCCGAA AAAGTCCCTA 
TGTGTTCTGC TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261295.1
CDS	606..1160
Translation	MREYKIVVLGSGGVGKSALTVQFVQCIFVEKYDPTIEDSYRKQVEVDGQQCMLEILDTAGTEQFTAMRDLYMKNGQGFVLVYSITAQSTFNDLQDLREQILRVKDTDDVPMVLVGNKCDLEEERVVGKELGKNLATQFNCAFMETSAKAKVNVNDIFYDLVRQINKKSPEKKQKKPKKSLCVLL

Target ORF information:

RefSeq Version	NM_001274366.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rap1 GTPase (Rap1), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274366.1

ORF Insert Sequence:

ATGCGTGAGT ACAAAATCGT GGTCCTTGGA AGCGGCGGCG TGGGCAAATC CGCGCTGACA 
GTCCAGTTTG TCCAGTGCAT CTTCGTGGAG AAGTACGATC CCACCATCGA GGACAGTTAC 
AGGAAGCAGG TGGAGGTGGA CGGACAGCAG TGCATGCTGG AGATCCTGGA CACGGCGGGT 
ACGGAGCAGT TCACGGCCAT GCGGGATTTG TACATGAAGA ACGGCCAGGG ATTCGTGCTG 
GTCTACTCGA TCACGGCGCA ATCGACGTTT AACGATCTGC AGGACCTGCG CGAGCAGATA 
CTCCGGGTGA AGGACACGGA CGATGTGCCC ATGGTGCTCG TGGGCAACAA GTGCGACCTC 
GAAGAGGAGC GCGTCGTCGG CAAGGAGCTG GGCAAGAACC TGGCCACCCA GTTCAACTGC 
GCCTTCATGG AGACCTCAGC CAAAGCCAAA GTGAATGTGA ACGATATTTT CTACGACCTG 
GTCCGGCAGA TCAACAAGAA GTCGCCCGAG AAGAAACAGA AGAAGCCGAA AAAGTCCCTA 
TGTGTTCTGC TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Rap1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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