alpha-Spec cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following alpha-Spec gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the alpha-Spec cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa17228 NM_057391.4
Latest version!
Drosophila melanogaster alpha spectrin, transcript variant A (alpha-Spec), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OFa20437 NM_001274359.1
Latest version!
Drosophila melanogaster alpha spectrin, transcript variant D (alpha-Spec), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OFa20438 NM_001274358.1
Latest version!
Drosophila melanogaster alpha spectrin, transcript variant C (alpha-Spec), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OFa20439 NM_001274357.1
Latest version!
Drosophila melanogaster alpha spectrin, transcript variant B (alpha-Spec), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa17228
    Clone ID Related Accession (Same CDS sequence) NM_057391.4
    Accession Version NM_057391.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7248bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product alpha spectrin, isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_057391.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGAGAACT TTACACCCAA AGAGGTGAAG ATCCTCGAGA CAGTCGAGGA CATCCAGGAG 
    CGACGTGAGC AGGTTCTGTC CCGCTACAAT GACTTCAAGA TCGAGACGCG CCAGAAGCGT
    GAAAAGCTCG AGGACTCGCG CCGCTTCCAG TACTTCAAGC GCGATGCCGA CGAGTTGGAG
    TCGTGGATCC ACGAGAAGCT GCAGGCAGCC AGCGAGGAGA GCTACCGCGA TCCGACCAAC
    CTGCAGGCCA AAATCCAGAA ACATCAGGCG TTCGAGGCGG AGGTGTCGGC GCACAGCAAC
    GCCATTGTCT CGCTGGACAA CACTGGCCAG GAGATGATCA ACCAGCAACA TTTTGCCTCC
    GAGTCGATTC AGGTCCGCCT CGACGAGCTG CACAAGCTGT GGGAGCTGCT TCTTAGCCGT
    CTGGCCGAGA AGGGTCTAAA GCTGCAGCAG GCTCTCGTCC TGGTGCAGTT CCTGCGCCAG
    TGCGAGGAGG TGATGTTCTG GATTAAGGAC AAGGAGACCT TTGTCACCGC CGACGAGTTC
    GGCCAAGACC TGGAACATGT AGAGGTTCTG CAGCGCAAGT TCGATGAGTT CCAGAAGGAC
    ATGGCCTCCC AGGAGTATCG CGTGACTGAG GTCAACCAGT TGGCCGATAA GTTGGTGCAA
    GATGGTCATC CCGAGCGCGA CACTATTACC AAGCGCAAGG AGGAGCTAAA CGAGGCTTGG
    CAGCGACTTA AGCAGTTAGC CATTGTGCGC CAAGAGAAGC TATTCGGTGC CCACGAGATC
    CAGCGTTTCA ATCGCGATGC CGACGAAACC GTCGCCTGGA TTGCCGAGAA GGATGTGGTC
    CTGTCGTCCG ATGATTATGG CCGCGATTTG GCCAGTGTTC AGGCGCTGCA ACGCAAACAC
    GAAGGAGTGG AGCGGGATTT GGCCGCCTTG GAGGATAAGG TTTCCACACT GGGAGCCGAG
    GCCCAGCGGC TGTGCTCCAT TCACGCCGAT CACAGTGACC AGATCCGTGA CAAACAGGCC
    GAGATTGCCA ACTACTGGCA GAGTCTCACC ACCAAGGCTC GCGAGCGCAA GCAGAAGCTA
    GATGAGTCCT ACTACCTGCA CCGTTTCCTC GCCGATTTCC GCGACCTCGT GTCCTGGATC
    AATGGCATGA AGGCCATCAT CTCGGCCGAT GAGCTGGCTA AGGATGTGGC TGGAGCAGAG
    GCTCTTCTGG AACGTCACCA GGAGCACAAG GGCGAGATCG ACGCTCGTGA GGATAGCTTC
    AAGCTGACCA CTGAATCCGG ACAGAAGCTG CTGGAGCGTG AGCATTACGC CGCCGCCGAA
    ATCCAGGAAA AGCTTGCCGC TCTGGAAAAC GACAAGAGTT CACTGCTCTC GCTGTGGGAA
    GATCGTCGCA TCCTGTACGA GCAGTGCATG GATCTGCAAC TGTTTTACCG CGATACGGAG
    CAGGCTGACA CTTGGATGGC CAAACAGGAG GCCTTCCTGG CCAACGAGGA CCTTGGCGAC
    TCCCTGGACT CTGTGGAGGC GCTAATTAAG AAGCACGAGG ACTTCGAGAA GAGTCTGGCT
    GCCCAGGAGG AGAAGATCAA GGCTTTAGAC ATCTTTGCCA CCAAATTAAT CGATGGTCAG
    CATTATGCCG CCGATGATGT GGCCCAGCGC CGTCAGATGC TCCTAGCCCG GCGGGCTGCT
    CTCCAAGAAA AATCGTCCAA GCGTCGCCAA TTGCTGGAGG ATTCGAACCG CTACCAGCAG
    TTCGAGCGCG ATTGCGACGA GACCAAGGGC TGGATTAGCG AGAAGCTGAA GTTTGCCACC
    GATGACAGCT ATTTGGATCC TACCAACCTT AACGGAAAGA TGCAGAAGCA CCAGAACTTT
    GAGCACGAGC TGAATGCCAA CAAGTCGCGC ATCGAGGACA TCACCAACGT GGGCACCGAG
    CTGATCGAGA AGCAGCACTA TGCTGCCGAC CAGATCAACA CCCGCATGCA GGAGATCGTC
    GTCCTGTGGG AGACCCTCGT CCAGGCTTCC GATAAAAAGG GAACCAAACT CAACGAGGCA
    TGCCAACAGC AGCAGTTCAA CCGCACCATC GAGGACATTG AGCTGTGGCT TAGCGAGATC
    GAGGGTCAAT TGTTGTCGGA GGATCACGGC AAAGACCTGA CCTCCGTTCA GAATTTGCAG
    AAGAAGCACG CCCTGCTGGA GGCCGATGTG ATGGCTCACC AGGATCGCAT TGAAAGCATC
    AAAGTGGCCG CCAACAAGTT CATCGAATCC GGTCACTTTG ACGCGGACAA CATTCGCAAC
    AAGGAGGGCA ATCTGTCGGC TCGCTATGCC GCCCTAGCTG CACCCATGGG CGAGAGGAAA
    CAACACCTTC TTGACTCCCT TCAGGTGCAG CAGCTCTTCC GCGACTTGGA GGACGAGGCT
    GCCTGGATCC GTGAGAAGGA ACCCATTGCC GCATCCACGA ACCGCGGTCG TGACTTGATT
    GGTGTGCAAA ACCTGATCAA GAAACACCAG GCTGTGCTGG CCGAGATCAA CAACCATGAG
    GCCAGGCTGC TGAATGTGAT CAGTTCGGGC GAGAACATGC TGAAGGACCA GCCCTTCGCC
    AGCGACGATA TCCGTCAGCG TTTGGAGGCG CTCCAAGAGC AGTGGAACAC GCTGAAGGAG
    AAGTCCAGCC AGCGAAAGCA GGATCTGGAT GACTCTCTCC AGGCACACCA GTACTTTGCC
    GACGCCAACG AAGCTGAGTC GTGGATGCGC GAAAAGGAAC CGATTGCAAC TGGCAGTGAT
    TATGGTAAGG ACGAGGATTC CTCGGAGGCT TTGCTCAAGA AGCACGAGGC TCTGGTCTCC
    GATCTAGAGG CTTTTGGCAA CACCATTCAG GCGCTGCAGG AGCAGGCCAA GAACTGTCGC
    CAGCAGGAGA CCCCTGTTGT GGATATCACT GGCAAGGAGT GCGTGGTTGC CCTCTACGAC
    TACACGGAGA AATCCCCTCG TGAGGTCTCG ATGAAGAAGG GCGATGTTCT GACCCTGCTG
    AACTCGAACA ACAAAGACTG GTGGAAGGTG GAGGTCAACG ACCGCCAGGG CTTCGTACCG
    GCCGCATACA TCAAGAAGAT TGACGCCGGC TTGAGCGCCT CCCAGCAGAA CCTAGTGGAC
    AATCATTCGA TTGCCAAGCG CCAGAACCAG ATCAACTCGC AGTACGACAA CTTGCTGGCC
    CTCGCCCGCG AGCGCCAGAA CAAGCTAAAC GAGACTGTGA AGGCGTACGT TCTTGTTCGT
    GAGGCTGCCG ACTTGGCCCA GTGGATCCGG GACAAGGAGA ACCACGCTCA GATCGCTGAT
    GTTGTGGGAG AGGATCTGGA AGAGGTGGAG GTGCTCCAAA AGAAGTTCGA CGACTTCAAC
    GACGACCTGA AGGCCAACGA AGTGCGTCTG GCAAACATGA ACGAGATTGC CGTGCAGCTG
    ACTTCCTTGG GTCAGACCGA GGCCGCCCTA AAGATCCAAA CCCAGATGCA AGACCTGAAC
    GAGAAGTGGA ACAACCTGCA GACGCTGACC GCTGAGAAGG CCAGCCAACT GGGATCCGCC
    CATGAGGTGC AACGCTTCCA TCGTGACATT GACGAGACCA AGGATTGGAT CGCTGAGAAG
    GCCAATGCCC TGAACAACGA CGACCTGGGC AAGGATCTGC GCAGTGTCCA GACTCTGCAG
    CGCAAGCACG AGGGCGTGGA ACGCGACTTG GCCGCATTGC GGGACAAGAT CCGCCAGCTG
    GACGAAACCG CCAACAGGCT GATGCAGTCG CACCCGGACA CCGCCGAGCA GACCTACGCC
    AAGCAGAAGG AGATCAACGA GATGTGGGAC CAGATCATCA CCAAGTCCAC CGCCCGCAAG
    GAGAAGCTGC TGGACTCGTA CGACCTGCAG CGTTTCCTCA GCGACTACCG CGATCTGCTG
    GCCTGGATCA ACTCGATGAT GAGCCTGGTC ACGTCCGACG AGCTGGCCAA CGATGTAACA
    GGAGCCGAGG CTCTGATCGA GCGTCATCAG GAACACCGAA CGGAGATCGA TGCGCGCGCC
    GGAACTTTCG GGGCTTTCGA GCAGTTCGGA AACGAGCTGC TGCAGGCCAA CCACTATGCG
    TCGCCTGAAA TCAAGGAGAA AATCGAGGAT CTGGCCAAGG CCCGCGAGGA CTTGGAGAAG
    GCGTGGACAG AGCGCCGCCT GCAGCTGGAG CAGAACTTGG ATCTGCAGCT CTACATGCGT
    GACTGCGAGT TGGCCGAGTC CTGGATGTCA GCCCGCGAGG CCTTCCTAAA TGCCGATGAT
    GATGCCAATG CCGGCGGAAA TGTGGAGGCA CTGATCAAGA AGCACGAGGA CTTCGACAAG
    GCCATTAACG GCCACGAACA GAAGATTGCT GCTCTGCAGA CGGTGGCGGA TCAGTTGATC
    GCCCAGAACC ACTATGCCTC CAATTTGGTG GACGAGAAGC GCAAGCAAGT GCTGGAGCGC
    TGGCGCCATC TCAAGGAGGG TCTGATCGAG AAGCGATCCC GTTTGGGTGA CGAGCAGACG
    CTGCAGCAGT TCTCGCGCGA TGCCGATGAG ATTGAGAACT GGATTGCAGA GAAGTTGCAG
    CTGGCCACCG AAGAGAGCTA CAAGGACCCG GCTAACATTC AGTCGAAGCA CCAGAAGCAC
    CAGGCCTTCG AGGCGGAGCT GGCGGCTAAC GCCGATCGCA TCCAGAGCGT GCTAGCCATG
    GGCGGAAACC TCATCGATAA GAAGCAGTGC AGCGGGTCGG AGGACGCGGT GCAGAAGCGG
    CTGACGCAGA TCGCCGATCA GTGGGAGTAC CTGACCCATA AGACTACCGA GAAGTCGCTG
    AAGCTAAAGG AGGCGAATAA GCAGCGCACC TATATCGCTG CTGTCAAGGA TTTGGACTTC
    TGGTTGGGCG AGGTCGAGAG TCTTTTGACC ACTGAGGATT CTGGTAAGGA TTTGGCCTCT
    GTCCAAAACC TCATGAAGAA GCATCAGTTG GTCGAGGCGG ATATCGTGGC GCACGAAGAC
    CGCATCAAGG ACATGAACAA CCAGGCCGAT TCCTTGGTGG AGAGCGGCCA GTTCGATACT
    GCCGGCATCC AGGAGAAGCG TCAAAGCATC AATGAGCGCT ACGAGCGCAT CTGCAACCTG
    GCGGCCCATC GCCAAGCCCG TCTCAACGAG GCTCTGACCC TGCACCAATT CTTCCGCGAC
    ATCGCCGACG AGGAGAGCTG GATCAAGGAG AAGAAGCTAC TTGTCGGCTC CGACGACTAT
    GGTCGCGATC TGACCGGCGT CCAGAACCTC AAGAAGAAGC ACAAGCGCCT AGAAGCCGAA
    TTGGGCTCTC ACGAGCCGGC CATTCAAGCT GTGCAGGAGG CTGGCGAGAA GCTCATGGAC
    GTGTCCAACC TCGGTGTCCC CGAAATTGAG CAGCGCCTGA AGGCTCTGAA CCAGGCCTGG
    GCCGAGCTAA AGAACCTGGC TGCCACGCGA GGTCAGAAGT TGGATGAGTC GCTGACCTAT
    CAGCAATTCC TCGCTCAGGT GGAGGAGGAG GAGGCCTGGA TCACCGAGAA GCAACAGCTG
    CTCTCTGTGG AGGACTACGG CGACTCGATG GCCGCCGTAC AAGGACTGCT GAAAAAGCAC
    GACGCATTCG AAACGGACTT CACTGCCCAC AAGGACCGCT GTTCTTTGAT CTGCGACCAG
    GGCAGTGAGT TGGTGGAGGC CAAGAACCAC CACGGAGAGT CCATTGCCCA ACGCTGCCAG
    CAGCTGCGCC TGAAACTGGA CAACCTGTCC GCCCTGGCTG CCCGCCGCAA GGGTGCCCTG
    CTGGACAACT CGGCCTATCT GCAGTTCATG TGGAAGGCCG ATGTGGTCGA GAGCTGGATC
    GACGACAAGG AGAACTATGT GCGCTCCGAT GAGTTCGGAC GCGACCTGTC CACCGTGCAA
    ACGCTGTTGA CCAAGCAAGA GACATTCGAT GCAGGTCTCA ATGCCTTCGA GCAGGAGGGC
    ATACACAACA TCACGGCACT TAAGGACCAA CTGATAAATG CCAGTCACGC CCAGTCGCCG
    GCAATCCTAA AGCGTCACGG AGACGTGATT GCCAGGTGGC AGAAGTTGCG CGACGCCTCC
    AACACGCGCA AGGACCGACT GCTGGCCATG CAGGAGCAGT TCCGCCAGAT CGAGGAACTC
    TACTTGACAT TTGCCAAGAA AGCTTCGGCC TTCAATTCTT GGTTCGAAAA TGCCGAGGAG
    GATCTCACAG ATCCTGTTCG CTGCAATTCG ATCGAAGAAA TCCGCGCCCT GCGCGACGCC
    CATGCCCAAT TCCAGGCGTC CCTTTCGTCG GCCGAAGCTG ACTTCAAGGC ATTGGCAGCA
    CTCGACCAGA AGATCAAGAG CTTTAATGTT GGACCCAACC CGTACACGTG GTTCACCATG
    GAAGCTCTTG AGGAGACCTG GCGTAATCTG CAAAAGATCA TAGAGGAACG CGATGGGGAA
    CTTGCCAAGG AGGCCAAGCG GCAGGAGGAG AACGACAAGC TGCGCAAGGA GTTTGCCAAG
    CACGCCAATC TTTTCCACCA GTGGCTAACA GAGACAAGAA CGTCCATGAT GGAAGGCTCC
    GGTTCCCTGG AACAGCAACT GGAGGCGCTT CGTGTCAAGG CCACCGAGGT GCGCGCACGT
    CGCGTTGACC TCAAGAAGAT CGAGGAGCTG GGAGCCTTGC TGGAGGAGCA CCTTATTCTG
    GACAACCGCT ACACGGAACA CTCGACGGTG GGCCTGGCCC AGCAGTGGGA TCAGCTTGAC
    CAGCTGTCTA TGCGCATGCA GCACAACTTA GAGCAACAGA TCCAGGCACG CAACCACTCG
    GGCGTCTCCG AGGACTCACT CAAGGAGTTC TCGATGATGT TCAAGCACTT CGATAAGGAC
    AAGAGCGGCA AGCTCAACCA CCAGGAATTC AAGTCCTGCC TGCGCGCCCT CGGCTACGAC
    CTGCCCATGG TGGAGGAGGG ACAGCCCGAC CCGGAGTTCG AAGCAATTCT CGATGTTGTC
    GACCCCAACC GCGATGGCTA CGTCTCTCTG CAGGAATACA TCGCGTTCAT GATCTCCAAG
    GAGACGGAGA ACGTTCAATC CTACGAGGAG ATCGAGAATG CCTTCCGCGC CATCACCGCT
    GCCGACCGCC CCTATGTAAC TAAGGAGGAG CTCTACTGCA ACCTCACCAA GGACATGGCT
    GACTACTGCG TGCAGCGCAT GAAACCCTTC TCGGAACCGC GCTCCGGCCA GCCCATCAAG
    GATGCCCTGG ACTACATAGA CTTCACGCGA ACGCTGTTCC AGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_476739.1
    CDS277..7524
    Translation

    Target ORF information:

    RefSeq Version NM_057391.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster alpha spectrin, transcript variant A (alpha-Spec), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_057391.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGAGAACT TTACACCCAA AGAGGTGAAG ATCCTCGAGA CAGTCGAGGA CATCCAGGAG 
    CGACGTGAGC AGGTTCTGTC CCGCTACAAT GACTTCAAGA TCGAGACGCG CCAGAAGCGT
    GAAAAGCTCG AGGACTCGCG CCGCTTCCAG TACTTCAAGC GCGATGCCGA CGAGTTGGAG
    TCGTGGATCC ACGAGAAGCT GCAGGCAGCC AGCGAGGAGA GCTACCGCGA TCCGACCAAC
    CTGCAGGCCA AAATCCAGAA ACATCAGGCG TTCGAGGCGG AGGTGTCGGC GCACAGCAAC
    GCCATTGTCT CGCTGGACAA CACTGGCCAG GAGATGATCA ACCAGCAACA TTTTGCCTCC
    GAGTCGATTC AGGTCCGCCT CGACGAGCTG CACAAGCTGT GGGAGCTGCT TCTTAGCCGT
    CTGGCCGAGA AGGGTCTAAA GCTGCAGCAG GCTCTCGTCC TGGTGCAGTT CCTGCGCCAG
    TGCGAGGAGG TGATGTTCTG GATTAAGGAC AAGGAGACCT TTGTCACCGC CGACGAGTTC
    GGCCAAGACC TGGAACATGT AGAGGTTCTG CAGCGCAAGT TCGATGAGTT CCAGAAGGAC
    ATGGCCTCCC AGGAGTATCG CGTGACTGAG GTCAACCAGT TGGCCGATAA GTTGGTGCAA
    GATGGTCATC CCGAGCGCGA CACTATTACC AAGCGCAAGG AGGAGCTAAA CGAGGCTTGG
    CAGCGACTTA AGCAGTTAGC CATTGTGCGC CAAGAGAAGC TATTCGGTGC CCACGAGATC
    CAGCGTTTCA ATCGCGATGC CGACGAAACC GTCGCCTGGA TTGCCGAGAA GGATGTGGTC
    CTGTCGTCCG ATGATTATGG CCGCGATTTG GCCAGTGTTC AGGCGCTGCA ACGCAAACAC
    GAAGGAGTGG AGCGGGATTT GGCCGCCTTG GAGGATAAGG TTTCCACACT GGGAGCCGAG
    GCCCAGCGGC TGTGCTCCAT TCACGCCGAT CACAGTGACC AGATCCGTGA CAAACAGGCC
    GAGATTGCCA ACTACTGGCA GAGTCTCACC ACCAAGGCTC GCGAGCGCAA GCAGAAGCTA
    GATGAGTCCT ACTACCTGCA CCGTTTCCTC GCCGATTTCC GCGACCTCGT GTCCTGGATC
    AATGGCATGA AGGCCATCAT CTCGGCCGAT GAGCTGGCTA AGGATGTGGC TGGAGCAGAG
    GCTCTTCTGG AACGTCACCA GGAGCACAAG GGCGAGATCG ACGCTCGTGA GGATAGCTTC
    AAGCTGACCA CTGAATCCGG ACAGAAGCTG CTGGAGCGTG AGCATTACGC CGCCGCCGAA
    ATCCAGGAAA AGCTTGCCGC TCTGGAAAAC GACAAGAGTT CACTGCTCTC GCTGTGGGAA
    GATCGTCGCA TCCTGTACGA GCAGTGCATG GATCTGCAAC TGTTTTACCG CGATACGGAG
    CAGGCTGACA CTTGGATGGC CAAACAGGAG GCCTTCCTGG CCAACGAGGA CCTTGGCGAC
    TCCCTGGACT CTGTGGAGGC GCTAATTAAG AAGCACGAGG ACTTCGAGAA GAGTCTGGCT
    GCCCAGGAGG AGAAGATCAA GGCTTTAGAC ATCTTTGCCA CCAAATTAAT CGATGGTCAG
    CATTATGCCG CCGATGATGT GGCCCAGCGC CGTCAGATGC TCCTAGCCCG GCGGGCTGCT
    CTCCAAGAAA AATCGTCCAA GCGTCGCCAA TTGCTGGAGG ATTCGAACCG CTACCAGCAG
    TTCGAGCGCG ATTGCGACGA GACCAAGGGC TGGATTAGCG AGAAGCTGAA GTTTGCCACC
    GATGACAGCT ATTTGGATCC TACCAACCTT AACGGAAAGA TGCAGAAGCA CCAGAACTTT
    GAGCACGAGC TGAATGCCAA CAAGTCGCGC ATCGAGGACA TCACCAACGT GGGCACCGAG
    CTGATCGAGA AGCAGCACTA TGCTGCCGAC CAGATCAACA CCCGCATGCA GGAGATCGTC
    GTCCTGTGGG AGACCCTCGT CCAGGCTTCC GATAAAAAGG GAACCAAACT CAACGAGGCA
    TGCCAACAGC AGCAGTTCAA CCGCACCATC GAGGACATTG AGCTGTGGCT TAGCGAGATC
    GAGGGTCAAT TGTTGTCGGA GGATCACGGC AAAGACCTGA CCTCCGTTCA GAATTTGCAG
    AAGAAGCACG CCCTGCTGGA GGCCGATGTG ATGGCTCACC AGGATCGCAT TGAAAGCATC
    AAAGTGGCCG CCAACAAGTT CATCGAATCC GGTCACTTTG ACGCGGACAA CATTCGCAAC
    AAGGAGGGCA ATCTGTCGGC TCGCTATGCC GCCCTAGCTG CACCCATGGG CGAGAGGAAA
    CAACACCTTC TTGACTCCCT TCAGGTGCAG CAGCTCTTCC GCGACTTGGA GGACGAGGCT
    GCCTGGATCC GTGAGAAGGA ACCCATTGCC GCATCCACGA ACCGCGGTCG TGACTTGATT
    GGTGTGCAAA ACCTGATCAA GAAACACCAG GCTGTGCTGG CCGAGATCAA CAACCATGAG
    GCCAGGCTGC TGAATGTGAT CAGTTCGGGC GAGAACATGC TGAAGGACCA GCCCTTCGCC
    AGCGACGATA TCCGTCAGCG TTTGGAGGCG CTCCAAGAGC AGTGGAACAC GCTGAAGGAG
    AAGTCCAGCC AGCGAAAGCA GGATCTGGAT GACTCTCTCC AGGCACACCA GTACTTTGCC
    GACGCCAACG AAGCTGAGTC GTGGATGCGC GAAAAGGAAC CGATTGCAAC TGGCAGTGAT
    TATGGTAAGG ACGAGGATTC CTCGGAGGCT TTGCTCAAGA AGCACGAGGC TCTGGTCTCC
    GATCTAGAGG CTTTTGGCAA CACCATTCAG GCGCTGCAGG AGCAGGCCAA GAACTGTCGC
    CAGCAGGAGA CCCCTGTTGT GGATATCACT GGCAAGGAGT GCGTGGTTGC CCTCTACGAC
    TACACGGAGA AATCCCCTCG TGAGGTCTCG ATGAAGAAGG GCGATGTTCT GACCCTGCTG
    AACTCGAACA ACAAAGACTG GTGGAAGGTG GAGGTCAACG ACCGCCAGGG CTTCGTACCG
    GCCGCATACA TCAAGAAGAT TGACGCCGGC TTGAGCGCCT CCCAGCAGAA CCTAGTGGAC
    AATCATTCGA TTGCCAAGCG CCAGAACCAG ATCAACTCGC AGTACGACAA CTTGCTGGCC
    CTCGCCCGCG AGCGCCAGAA CAAGCTAAAC GAGACTGTGA AGGCGTACGT TCTTGTTCGT
    GAGGCTGCCG ACTTGGCCCA GTGGATCCGG GACAAGGAGA ACCACGCTCA GATCGCTGAT
    GTTGTGGGAG AGGATCTGGA AGAGGTGGAG GTGCTCCAAA AGAAGTTCGA CGACTTCAAC
    GACGACCTGA AGGCCAACGA AGTGCGTCTG GCAAACATGA ACGAGATTGC CGTGCAGCTG
    ACTTCCTTGG GTCAGACCGA GGCCGCCCTA AAGATCCAAA CCCAGATGCA AGACCTGAAC
    GAGAAGTGGA ACAACCTGCA GACGCTGACC GCTGAGAAGG CCAGCCAACT GGGATCCGCC
    CATGAGGTGC AACGCTTCCA TCGTGACATT GACGAGACCA AGGATTGGAT CGCTGAGAAG
    GCCAATGCCC TGAACAACGA CGACCTGGGC AAGGATCTGC GCAGTGTCCA GACTCTGCAG
    CGCAAGCACG AGGGCGTGGA ACGCGACTTG GCCGCATTGC GGGACAAGAT CCGCCAGCTG
    GACGAAACCG CCAACAGGCT GATGCAGTCG CACCCGGACA CCGCCGAGCA GACCTACGCC
    AAGCAGAAGG AGATCAACGA GATGTGGGAC CAGATCATCA CCAAGTCCAC CGCCCGCAAG
    GAGAAGCTGC TGGACTCGTA CGACCTGCAG CGTTTCCTCA GCGACTACCG CGATCTGCTG
    GCCTGGATCA ACTCGATGAT GAGCCTGGTC ACGTCCGACG AGCTGGCCAA CGATGTAACA
    GGAGCCGAGG CTCTGATCGA GCGTCATCAG GAACACCGAA CGGAGATCGA TGCGCGCGCC
    GGAACTTTCG GGGCTTTCGA GCAGTTCGGA AACGAGCTGC TGCAGGCCAA CCACTATGCG
    TCGCCTGAAA TCAAGGAGAA AATCGAGGAT CTGGCCAAGG CCCGCGAGGA CTTGGAGAAG
    GCGTGGACAG AGCGCCGCCT GCAGCTGGAG CAGAACTTGG ATCTGCAGCT CTACATGCGT
    GACTGCGAGT TGGCCGAGTC CTGGATGTCA GCCCGCGAGG CCTTCCTAAA TGCCGATGAT
    GATGCCAATG CCGGCGGAAA TGTGGAGGCA CTGATCAAGA AGCACGAGGA CTTCGACAAG
    GCCATTAACG GCCACGAACA GAAGATTGCT GCTCTGCAGA CGGTGGCGGA TCAGTTGATC
    GCCCAGAACC ACTATGCCTC CAATTTGGTG GACGAGAAGC GCAAGCAAGT GCTGGAGCGC
    TGGCGCCATC TCAAGGAGGG TCTGATCGAG AAGCGATCCC GTTTGGGTGA CGAGCAGACG
    CTGCAGCAGT TCTCGCGCGA TGCCGATGAG ATTGAGAACT GGATTGCAGA GAAGTTGCAG
    CTGGCCACCG AAGAGAGCTA CAAGGACCCG GCTAACATTC AGTCGAAGCA CCAGAAGCAC
    CAGGCCTTCG AGGCGGAGCT GGCGGCTAAC GCCGATCGCA TCCAGAGCGT GCTAGCCATG
    GGCGGAAACC TCATCGATAA GAAGCAGTGC AGCGGGTCGG AGGACGCGGT GCAGAAGCGG
    CTGACGCAGA TCGCCGATCA GTGGGAGTAC CTGACCCATA AGACTACCGA GAAGTCGCTG
    AAGCTAAAGG AGGCGAATAA GCAGCGCACC TATATCGCTG CTGTCAAGGA TTTGGACTTC
    TGGTTGGGCG AGGTCGAGAG TCTTTTGACC ACTGAGGATT CTGGTAAGGA TTTGGCCTCT
    GTCCAAAACC TCATGAAGAA GCATCAGTTG GTCGAGGCGG ATATCGTGGC GCACGAAGAC
    CGCATCAAGG ACATGAACAA CCAGGCCGAT TCCTTGGTGG AGAGCGGCCA GTTCGATACT
    GCCGGCATCC AGGAGAAGCG TCAAAGCATC AATGAGCGCT ACGAGCGCAT CTGCAACCTG
    GCGGCCCATC GCCAAGCCCG TCTCAACGAG GCTCTGACCC TGCACCAATT CTTCCGCGAC
    ATCGCCGACG AGGAGAGCTG GATCAAGGAG AAGAAGCTAC TTGTCGGCTC CGACGACTAT
    GGTCGCGATC TGACCGGCGT CCAGAACCTC AAGAAGAAGC ACAAGCGCCT AGAAGCCGAA
    TTGGGCTCTC ACGAGCCGGC CATTCAAGCT GTGCAGGAGG CTGGCGAGAA GCTCATGGAC
    GTGTCCAACC TCGGTGTCCC CGAAATTGAG CAGCGCCTGA AGGCTCTGAA CCAGGCCTGG
    GCCGAGCTAA AGAACCTGGC TGCCACGCGA GGTCAGAAGT TGGATGAGTC GCTGACCTAT
    CAGCAATTCC TCGCTCAGGT GGAGGAGGAG GAGGCCTGGA TCACCGAGAA GCAACAGCTG
    CTCTCTGTGG AGGACTACGG CGACTCGATG GCCGCCGTAC AAGGACTGCT GAAAAAGCAC
    GACGCATTCG AAACGGACTT CACTGCCCAC AAGGACCGCT GTTCTTTGAT CTGCGACCAG
    GGCAGTGAGT TGGTGGAGGC CAAGAACCAC CACGGAGAGT CCATTGCCCA ACGCTGCCAG
    CAGCTGCGCC TGAAACTGGA CAACCTGTCC GCCCTGGCTG CCCGCCGCAA GGGTGCCCTG
    CTGGACAACT CGGCCTATCT GCAGTTCATG TGGAAGGCCG ATGTGGTCGA GAGCTGGATC
    GACGACAAGG AGAACTATGT GCGCTCCGAT GAGTTCGGAC GCGACCTGTC CACCGTGCAA
    ACGCTGTTGA CCAAGCAAGA GACATTCGAT GCAGGTCTCA ATGCCTTCGA GCAGGAGGGC
    ATACACAACA TCACGGCACT TAAGGACCAA CTGATAAATG CCAGTCACGC CCAGTCGCCG
    GCAATCCTAA AGCGTCACGG AGACGTGATT GCCAGGTGGC AGAAGTTGCG CGACGCCTCC
    AACACGCGCA AGGACCGACT GCTGGCCATG CAGGAGCAGT TCCGCCAGAT CGAGGAACTC
    TACTTGACAT TTGCCAAGAA AGCTTCGGCC TTCAATTCTT GGTTCGAAAA TGCCGAGGAG
    GATCTCACAG ATCCTGTTCG CTGCAATTCG ATCGAAGAAA TCCGCGCCCT GCGCGACGCC
    CATGCCCAAT TCCAGGCGTC CCTTTCGTCG GCCGAAGCTG ACTTCAAGGC ATTGGCAGCA
    CTCGACCAGA AGATCAAGAG CTTTAATGTT GGACCCAACC CGTACACGTG GTTCACCATG
    GAAGCTCTTG AGGAGACCTG GCGTAATCTG CAAAAGATCA TAGAGGAACG CGATGGGGAA
    CTTGCCAAGG AGGCCAAGCG GCAGGAGGAG AACGACAAGC TGCGCAAGGA GTTTGCCAAG
    CACGCCAATC TTTTCCACCA GTGGCTAACA GAGACAAGAA CGTCCATGAT GGAAGGCTCC
    GGTTCCCTGG AACAGCAACT GGAGGCGCTT CGTGTCAAGG CCACCGAGGT GCGCGCACGT
    CGCGTTGACC TCAAGAAGAT CGAGGAGCTG GGAGCCTTGC TGGAGGAGCA CCTTATTCTG
    GACAACCGCT ACACGGAACA CTCGACGGTG GGCCTGGCCC AGCAGTGGGA TCAGCTTGAC
    CAGCTGTCTA TGCGCATGCA GCACAACTTA GAGCAACAGA TCCAGGCACG CAACCACTCG
    GGCGTCTCCG AGGACTCACT CAAGGAGTTC TCGATGATGT TCAAGCACTT CGATAAGGAC
    AAGAGCGGCA AGCTCAACCA CCAGGAATTC AAGTCCTGCC TGCGCGCCCT CGGCTACGAC
    CTGCCCATGG TGGAGGAGGG ACAGCCCGAC CCGGAGTTCG AAGCAATTCT CGATGTTGTC
    GACCCCAACC GCGATGGCTA CGTCTCTCTG CAGGAATACA TCGCGTTCAT GATCTCCAAG
    GAGACGGAGA ACGTTCAATC CTACGAGGAG ATCGAGAATG CCTTCCGCGC CATCACCGCT
    GCCGACCGCC CCTATGTAAC TAAGGAGGAG CTCTACTGCA ACCTCACCAA GGACATGGCT
    GACTACTGCG TGCAGCGCAT GAAACCCTTC TCGGAACCGC GCTCCGGCCA GCCCATCAAG
    GATGCCCTGG ACTACATAGA CTTCACGCGA ACGCTGTTCC AGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa20437
    Clone ID Related Accession (Same CDS sequence) NM_001274359.1
    Accession Version NM_001274359.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7278bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product alpha spectrin, isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGGAGAACT TTACACCCAA AGAGGTGAAG ATCCTCGAGA CAGTCGAGGA CATCCAGGAG 
    CGACGTGAGC AGGTTCTGTC CCGCTACAAT GACTTCAAGA TCGAGACGCG CCAGAAGCGT
    GAAAAGCTCG AGGACTCGCG CCGCTTCCAG TACTTCAAGC GCGATGCCGA CGAGTTGGAG
    TCGTGGATCC ACGAGAAGCT GCAGGCAGCC AGCGAGGAGA GCTACCGCGA TCCGACCAAC
    CTGCAGGCCA AAATCCAGAA ACATCAGGCG TTCGAGGCGG AGGTGTCGGC GCACAGCAAC
    GCCATTGTCT CGCTGGACAA CACTGGCCAG GAGATGATCA ACCAGCAACA TTTTGCCTCC
    GAGTCGATTC AGGTCCGCCT CGACGAGCTG CACAAGCTGT GGGAGCTGCT TCTTAGCCGT
    CTGGCCGAGA AGGGTCTAAA GCTGCAGCAG GCTCTCGTCC TGGTGCAGTT CCTGCGCCAG
    TGCGAGGAGG TGATGTTCTG GATTAAGGAC AAGGAGACCT TTGTCACCGC CGACGAGTTC
    GGCCAAGACC TGGAACATGT AGAGGTTCTG CAGCGCAAGT TCGATGAGTT CCAGAAGGAC
    ATGGCCTCCC AGGAGTATCG CGTGACTGAG GTCAACCAGT TGGCCGATAA GTTGGTGCAA
    GATGGTCATC CCGAGCGCGA CACTATTACC AAGCGCAAGG AGGAGCTAAA CGAGGCTTGG
    CAGCGACTTA AGCAGTTAGC CATTGTGCGC CAAGAGAAGC TATTCGGTGC CCACGAGATC
    CAGCGTTTCA ATCGCGATGC CGACGAAACC GTCGCCTGGA TTGCCGAGAA GGATGTGGTC
    CTGTCGTCCG ATGATTATGG CCGCGATTTG GCCAGTGTTC AGGCGCTGCA ACGCAAACAC
    GAAGGAGTGG AGCGGGATTT GGCCGCCTTG GAGGATAAGG TTTCCACACT GGGAGCCGAG
    GCCCAGCGGC TGTGCTCCAT TCACGCCGAT CACAGTGACC AGATCCGTGA CAAACAGGCC
    GAGATTGCCA ACTACTGGCA GAGTCTCACC ACCAAGGCTC GCGAGCGCAA GCAGAAGCTA
    GATGAGTCCT ACTACCTGCA CCGTTTCCTC GCCGATTTCC GCGACCTCGT GTCCTGGATC
    AATGGCATGA AGGCCATCAT CTCGGCCGAT GAGCTGGCTA AGGATGTGGC TGGAGCAGAG
    GCTCTTCTGG AACGTCACCA GGAGCACAAG GGCGAGATCG ACGCTCGTGA GGATAGCTTC
    AAGCTGACCA CTGAATCCGG ACAGAAGCTG CTGGAGCGTG AGCATTACGC CGCCGCCGAA
    ATCCAGGAAA AGCTTGCCGC TCTGGAAAAC GACAAGAGTT CACTGCTCTC GCTGTGGGAA
    GATCGTCGCA TCCTGTACGA GCAGTGCATG GATCTGCAAC TGTTTTACCG CGATACGGAG
    CAGGCTGACA CTTGGATGGC CAAACAGGAG GCCTTCCTGG CCAACGAGGA CCTTGGCGAC
    TCCCTGGACT CTGTGGAGGC GCTAATTAAG AAGCACGAGG ACTTCGAGAA GAGTCTGGCT
    GCCCAGGAGG AGAAGATCAA GGCTTTAGAC ATCTTTGCCA CCAAATTAAT CGATGGTCAG
    CATTATGCCG CCGATGATGT GGCCCAGCGC CGTCAGATGC TCCTAGCCCG GCGGGCTGCT
    CTCCAAGAAA AATCGTCCAA GCGTCGCCAA TTGCTGGAGG ATTCGAACCG CTACCAGCAG
    TTCGAGCGCG ATTGCGACGA GACCAAGGGC TGGATTAGCG AGAAGCTGAA GTTTGCCACC
    GATGACAGCT ATTTGGATCC TACCAACCTT AACGGAAAGA TGCAGAAGCA CCAGAACTTT
    GAGCACGAGC TGAATGCCAA CAAGTCGCGC ATCGAGGACA TCACCAACGT GGGCACCGAG
    CTGATCGAGA AGCAGCACTA TGCTGCCGAC CAGATCAACA CCCGCATGCA GGAGATCGTC
    GTCCTGTGGG AGACCCTCGT CCAGGCTTCC GATAAAAAGG GAACCAAACT CAACGAGGCA
    TGCCAACAGC AGCAGTTCAA CCGCACCATC GAGGACATTG AGCTGTGGCT TAGCGAGATC
    GAGGGTCAAT TGTTGTCGGA GGATCACGGC AAAGACCTGA CCTCCGTTCA GAATTTGCAG
    AAGAAGCACG CCCTGCTGGA GGCCGATGTG ATGGCTCACC AGGATCGCAT TGAAAGCATC
    AAAGTGGCCG CCAACAAGTT CATCGAATCC GGTCACTTTG ACGCGGACAA CATTCGCAAC
    AAGGAGGGCA ATCTGTCGGC TCGCTATGCC GCCCTAGCTG CACCCATGGG CGAGAGGAAA
    CAACACCTTC TTGACTCCCT TCAGGTGCAG CAGCTCTTCC GCGACTTGGA GGACGAGGCT
    GCCTGGATCC GTGAGAAGGA ACCCATTGCC GCATCCACGA ACCGCGGTCG TGACTTGATT
    GGTGTGCAAA ACCTGATCAA GAAACACCAG GCTGTGCTGG CCGAGATCAA CAACCATGAG
    GCCAGGCTGC TGAATGTGAT CAGTTCGGGC GAGAACATGC TGAAGGACCA GCCCTTCGCC
    AGCGACGATA TCCGTCAGCG TTTGGAGGCG CTCCAAGAGC AGTGGAACAC GCTGAAGGAG
    AAGTCCAGCC AGCGAAAGCA GGATCTGGAT GACTCTCTCC AGGCACACCA GTACTTTGCC
    GACGCCAACG AAGCTGAGTC GTGGATGCGC GAAAAGGAAC CGATTGCAAC TGGCAGTGAT
    TATGGTAAGG ACGAGGATTC CTCGGAGGCT TTGCTCAAGA AGCACGAGGC TCTGGTCTCC
    GATCTAGAGG CTTTTGGCAA CACCATTCAG GCGCTGCAGG AGCAGGCCAA GAACTGTCGC
    CAGCAGGAGA CCCCTGTTGT GGATATCACT GGCAAGGAGT GCGTGGTTGC CCTCTACGAC
    TACACGGAGA AATCCCCTCG TGAGGTCTCG ATGAAGAAGG GCGATGTTCT GACCCTGCTG
    AACTCGAACA ACAAAGACTG GTGGAAGGTG GAGGTCAACG ACCGCCAGGG CTTCGTACCG
    GCCGCATACA TCAAGAAGAT TGACGCCGGC TTGAGCGCCT CCCAGCAGAA CCTAGTGGAC
    AATCATTCGA TTGCCAAGCG CCAGAACCAG ATCAACTCGC AGTACGACAA CTTGCTGGCC
    CTCGCCCGCG AGCGCCAGAA CAAGCTAAAC GAGACTGTGA AGGCGTACGT TCTTGTTCGT
    GAGGCTGCCG ACTTGGCCCA GTGGATCCGG GACAAGGAGA ACCACGCTCA GATCGCTGAT
    GTTGTGGGAG AGGATCTGGA AGAGGTGGAG GTGCTCCAAA AGAAGTTCGA CGACTTCAAC
    GACGACCTGA AGGCCAACGA AGTGCGTCTG GCAAACATGA ACGAGATTGC CGTGCAGCTG
    ACTTCCTTGG GTCAGACCGA GGCCGCCCTA AAGATCCAAA CCCAGATGCA AGACCTGAAC
    GAGAAGTGGA ACAACCTGCA GACGCTGACC GCTGAGAAGG CCAGCCAACT GGGATCCGCC
    CATGAGGTGC AACGCTTCCA TCGTGACATT GACGAGACCA AGGATTGGAT CGCTGAGAAG
    GCCAATGCCC TGAACAACGA CGACCTGGGC AAGGATCTGC GCAGTGTCCA GACTCTGCAG
    CGCAAGCACG AGGGCGTGGA ACGCGACTTG GCCGCATTGC GGGACAAGAT CCGCCAGCTG
    GACGAAACCG CCAACAGGCT GATGCAGTCG CACCCGGACA CCGCCGAGCA GACCTACGCC
    AAGCAGAAGG AGATCAACGA GATGTGGGAC CAGATCATCA CCAAGTCCAC CGCCCGCAAG
    GAGAAGCTGC TGGACTCGTA CGACCTGCAG CGTTTCCTCA GCGACTACCG CGATCTGCTG
    GCCTGGATCA ACTCGATGAT GAGCCTGGTC ACGTCCGACG AGCTGGCCAA CGATGTAACA
    GGAGCCGAGG CTCTGATCGA GCGTCATCAG GAACACCGAA CGGAGATCGA TGCGCGCGCC
    GGAACTTTCG GGGCTTTCGA GCAGTTCGGA AACGAGCTGC TGCAGGCCAA CCACTATGCG
    TCGCCTGAAA TCAAGGAGAA AATCGAGGAT CTGGCCAAGG CCCGCGAGGA CTTGGAGAAG
    GCGTGGACAG AGCGCCGCCT GCAGCTGGAG CAGAACTTGG ATCTGCAGCT CTACATGCGT
    GACTGCGAGT TGGCCGAGTC CTGGATGTCA GCCCGCGAGG CCTTCCTAAA TGCCGATGAT
    GATGCCAATG CCGGCGGAAA TGTGGAGGCA CTGATCAAGA AGCACGAGGA CTTCGACAAG
    GCCATTAACG GCCACGAACA GAAGATTGCT GCTCTGCAGA CGGTGGCGGA TCAGTTGATC
    GCCCAGAACC ACTATGCCTC CAATTTGGTG GACGAGAAGC GCAAGCAAGT GCTGGAGCGC
    TGGCGCCATC TCAAGGAGGG TCTGATCGAG AAGCGATCCC GTTTGGGTGA CGAGCAGACG
    CTGCAGCAGT TCTCGCGCGA TGCCGATGAG ATTGAGAACT GGATTGCAGA GAAGTTGCAG
    CTGGCCACCG AAGAGAGCTA CAAGGACCCG GCTAACATTC AGTCGAAGCA CCAGAAGCAC
    CAGGCCTTCG AGGCGGAGCT GGCGGCTAAC GCCGATCGCA TCCAGAGCGT GCTAGCCATG
    GGCGGAAACC TCATCGATAA GAAGCAGTGC AGCGGGTCGG AGGACGCGGT GCAGAAGCGG
    CTGACGCAGA TCGCCGATCA GTGGGAGTAC CTGACCCATA AGACTACCGA GAAGTCGCTG
    AAGCTAAAGG AGGCGAATAA GCAGCGCACC TATATCGCTG CTGTCAAGGA TTTGGACTTC
    TGGTTGGGCG AGGTCGAGAG TCTTTTGACC ACTGAGGATT CTGGTAAGGA TTTGGCCTCT
    GTCCAAAACC TCATGAAGAA GCATCAGTTG GTCGAGGCGG ATATCGTGGC GCACGAAGAC
    CGCATCAAGG ACATGAACAA CCAGGCCGAT TCCTTGGTGG AGAGCGGCCA GTTCGATACT
    GCCGGCATCC AGGAGAAGCG TCAAAGCATC AATGAGCGCT ACGAGCGCAT CTGCAACCTG
    GCGGCCCATC GCCAAGCCCG TCTCAACGAG GCTCTGACCC TGCACCAATT CTTCCGCGAC
    ATCGCCGACG AGGAGAGCTG GATCAAGGAG AAGAAGCTAC TTGTCGGCTC CGACGACTAT
    GGTCGCGATC TGACCGGCGT CCAGAACCTC AAGAAGAAGC ACAAGCGCCT AGAAGCCGAA
    TTGGGCTCTC ACGAGCCGGC CATTCAAGCT GTGCAGGAGG CTGGCGAGAA GCTCATGGAC
    GTGTCCAACC TCGGTGTCCC CGAAATTGAG CAGCGCCTGA AGGCTCTGAA CCAGGCCTGG
    GCCGAGCTAA AGAACCTGGC TGCCACGCGA GGTCAGAAGT TGGATGAGTC GCTGACCTAT
    CAGCAATTCC TCGCTCAGGT GGAGGAGGAG GAGGCCTGGA TCACCGAGAA GCAACAGCTG
    CTCTCTGTGG AGGACTACGG CGACTCGATG GCCGCCGTAC AAGGACTGCT GAAAAAGCAC
    GACGCATTCG AAACGGACTT CACTGCCCAC AAGGACCGCT GTTCTTTGAT CTGCGACCAG
    GGCAGTGAGT TGGTGGAGGC CAAGAACCAC CACGGAGAGT CCATTGCCCA ACGCTGCCAG
    CAGCTGCGCC TGAAACTGGA CAACCTGTCC GCCCTGGCTG CCCGCCGCAA GGGTGCCCTG
    CTGGACAACT CGGCCTATCT GCAGTTCATG TGGAAGGCCG ATGTGGTCGA GAGCTGGATC
    GACGACAAGG AGAACTATGT GCGCTCCGAT GAGTTCGGAC GCGACCTGTC CACCGTGCAA
    ACGCTGTTGA CCAAGCAAGA GACATTCGAT GCAGGTCTCA ATGCCTTCGA GCAGGAGGGC
    ATACACAACA TCACGGCACT TAAGGACCAA CTGATAAATG CCAGTCACGC CCAGTCGCCG
    GCAATCCTAA AGCGTCACGG AGACGTGATT GCCAGGTGGC AGAAGTTGCG CGACGCCTCC
    AACACGCGCA AGGACCGACT GCTGGCCATG CAGGAGCAGT TCCGCCAGAT CGAGGAACTC
    TACTTGACAT TTGCCAAGAA AGCTTCGGCC TTCAATTCTT GGTTCGAAAA TGCCGAGGAG
    GATCTCACAG ATCCTGTTCG CTGCAATTCG ATCGAAGAAA TCCGCGCCCT GCGCGACGCC
    CATGCCCAAT TCCAGGCGTC CCTTTCGTCG GCCGAAGCTG ACTTCAAGGC ATTGGCAGCA
    CTCGACCAGA AGATCAAGAG CTTTAATGTT GGACCCAACC CGTACACGTG GTTCACCATG
    GAAGCTCTTG AGGAGACCTG GCGTAATCTG CAAAAGATCA TAGAGGAACG CGATGGGGAA
    CTTGCCAAGG AGGCCAAGCG GCAGGAGGAG AACGACAAGC TGCGCAAGGA GTTTGCCAAG
    CACGCCAATC TTTTCCACCA GTGGCTAACA GAGACAAGAT ATTATATGCT GGGTTATAAC
    CGTCAAGGAA CGTCCATGAT GGAAGGCTCC GGTTCCCTGG AACAGCAACT GGAGGCGCTT
    CGTGTCAAGG CCACCGAGGT GCGCGCACGT CGCGTTGACC TCAAGAAGAT CGAGGAGCTG
    GGAGCCTTGC TGGAGGAGCA CCTTATTCTG GACAACCGCT ACACGGAACA CTCGACGGTG
    GGCCTGGCCC AGCAGTGGGA TCAGCTTGAC CAGCTGTCTA TGCGCATGCA GCACAACTTA
    GAGCAACAGA TCCAGGCACG CAACCACTCG GGCGTCTCCG AGGACTCACT CAAGGAGTTC
    TCGATGATGT TCAAGCACTT CGATAAGGAC AAGAGCGGCA AGCTCAACCA CCAGGAATTC
    AAGTCCTGCC TGCGCGCCCT CGGCTACGAC CTGCCCATGG TGGAGGAGGG ACAGCCCGAC
    CCGGAGTTCG AAGCAATTCT CGATGTTGTC GACCCCAACC GCGATGGCTA CGTCTCTCTG
    CAGGAATACA TCGCGTTCAT GATCTCCAAG GAGACGGAGA ACGTTCAATC CTACGAGGAG
    ATCGAGAATG CCTTCCGCGC CATCACCGCT GCCGACCGCC CCTATGTAAC TAAGGAGGAG
    CTCTACTGCA ACCTCACCAA GGACATGGCT GACTACTGCG TGCAGCGCAT GAAACCCTTC
    TCGGAACCGC GCTCCGGCCA GCCCATCAAG GATGCCCTGG ACTACATAGA CTTCACGCGA
    ACGCTGTTCC AGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001261288.1
    CDS277..7554
    Translation

    Target ORF information:

    RefSeq Version NM_001274359.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster alpha spectrin, transcript variant D (alpha-Spec), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001274359.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGGAGAACT TTACACCCAA AGAGGTGAAG ATCCTCGAGA CAGTCGAGGA CATCCAGGAG 
    CGACGTGAGC AGGTTCTGTC CCGCTACAAT GACTTCAAGA TCGAGACGCG CCAGAAGCGT
    GAAAAGCTCG AGGACTCGCG CCGCTTCCAG TACTTCAAGC GCGATGCCGA CGAGTTGGAG
    TCGTGGATCC ACGAGAAGCT GCAGGCAGCC AGCGAGGAGA GCTACCGCGA TCCGACCAAC
    CTGCAGGCCA AAATCCAGAA ACATCAGGCG TTCGAGGCGG AGGTGTCGGC GCACAGCAAC
    GCCATTGTCT CGCTGGACAA CACTGGCCAG GAGATGATCA ACCAGCAACA TTTTGCCTCC
    GAGTCGATTC AGGTCCGCCT CGACGAGCTG CACAAGCTGT GGGAGCTGCT TCTTAGCCGT
    CTGGCCGAGA AGGGTCTAAA GCTGCAGCAG GCTCTCGTCC TGGTGCAGTT CCTGCGCCAG
    TGCGAGGAGG TGATGTTCTG GATTAAGGAC AAGGAGACCT TTGTCACCGC CGACGAGTTC
    GGCCAAGACC TGGAACATGT AGAGGTTCTG CAGCGCAAGT TCGATGAGTT CCAGAAGGAC
    ATGGCCTCCC AGGAGTATCG CGTGACTGAG GTCAACCAGT TGGCCGATAA GTTGGTGCAA
    GATGGTCATC CCGAGCGCGA CACTATTACC AAGCGCAAGG AGGAGCTAAA CGAGGCTTGG
    CAGCGACTTA AGCAGTTAGC CATTGTGCGC CAAGAGAAGC TATTCGGTGC CCACGAGATC
    CAGCGTTTCA ATCGCGATGC CGACGAAACC GTCGCCTGGA TTGCCGAGAA GGATGTGGTC
    CTGTCGTCCG ATGATTATGG CCGCGATTTG GCCAGTGTTC AGGCGCTGCA ACGCAAACAC
    GAAGGAGTGG AGCGGGATTT GGCCGCCTTG GAGGATAAGG TTTCCACACT GGGAGCCGAG
    GCCCAGCGGC TGTGCTCCAT TCACGCCGAT CACAGTGACC AGATCCGTGA CAAACAGGCC
    GAGATTGCCA ACTACTGGCA GAGTCTCACC ACCAAGGCTC GCGAGCGCAA GCAGAAGCTA
    GATGAGTCCT ACTACCTGCA CCGTTTCCTC GCCGATTTCC GCGACCTCGT GTCCTGGATC
    AATGGCATGA AGGCCATCAT CTCGGCCGAT GAGCTGGCTA AGGATGTGGC TGGAGCAGAG
    GCTCTTCTGG AACGTCACCA GGAGCACAAG GGCGAGATCG ACGCTCGTGA GGATAGCTTC
    AAGCTGACCA CTGAATCCGG ACAGAAGCTG CTGGAGCGTG AGCATTACGC CGCCGCCGAA
    ATCCAGGAAA AGCTTGCCGC TCTGGAAAAC GACAAGAGTT CACTGCTCTC GCTGTGGGAA
    GATCGTCGCA TCCTGTACGA GCAGTGCATG GATCTGCAAC TGTTTTACCG CGATACGGAG
    CAGGCTGACA CTTGGATGGC CAAACAGGAG GCCTTCCTGG CCAACGAGGA CCTTGGCGAC
    TCCCTGGACT CTGTGGAGGC GCTAATTAAG AAGCACGAGG ACTTCGAGAA GAGTCTGGCT
    GCCCAGGAGG AGAAGATCAA GGCTTTAGAC ATCTTTGCCA CCAAATTAAT CGATGGTCAG
    CATTATGCCG CCGATGATGT GGCCCAGCGC CGTCAGATGC TCCTAGCCCG GCGGGCTGCT
    CTCCAAGAAA AATCGTCCAA GCGTCGCCAA TTGCTGGAGG ATTCGAACCG CTACCAGCAG
    TTCGAGCGCG ATTGCGACGA GACCAAGGGC TGGATTAGCG AGAAGCTGAA GTTTGCCACC
    GATGACAGCT ATTTGGATCC TACCAACCTT AACGGAAAGA TGCAGAAGCA CCAGAACTTT
    GAGCACGAGC TGAATGCCAA CAAGTCGCGC ATCGAGGACA TCACCAACGT GGGCACCGAG
    CTGATCGAGA AGCAGCACTA TGCTGCCGAC CAGATCAACA CCCGCATGCA GGAGATCGTC
    GTCCTGTGGG AGACCCTCGT CCAGGCTTCC GATAAAAAGG GAACCAAACT CAACGAGGCA
    TGCCAACAGC AGCAGTTCAA CCGCACCATC GAGGACATTG AGCTGTGGCT TAGCGAGATC
    GAGGGTCAAT TGTTGTCGGA GGATCACGGC AAAGACCTGA CCTCCGTTCA GAATTTGCAG
    AAGAAGCACG CCCTGCTGGA GGCCGATGTG ATGGCTCACC AGGATCGCAT TGAAAGCATC
    AAAGTGGCCG CCAACAAGTT CATCGAATCC GGTCACTTTG ACGCGGACAA CATTCGCAAC
    AAGGAGGGCA ATCTGTCGGC TCGCTATGCC GCCCTAGCTG CACCCATGGG CGAGAGGAAA
    CAACACCTTC TTGACTCCCT TCAGGTGCAG CAGCTCTTCC GCGACTTGGA GGACGAGGCT
    GCCTGGATCC GTGAGAAGGA ACCCATTGCC GCATCCACGA ACCGCGGTCG TGACTTGATT
    GGTGTGCAAA ACCTGATCAA GAAACACCAG GCTGTGCTGG CCGAGATCAA CAACCATGAG
    GCCAGGCTGC TGAATGTGAT CAGTTCGGGC GAGAACATGC TGAAGGACCA GCCCTTCGCC
    AGCGACGATA TCCGTCAGCG TTTGGAGGCG CTCCAAGAGC AGTGGAACAC GCTGAAGGAG
    AAGTCCAGCC AGCGAAAGCA GGATCTGGAT GACTCTCTCC AGGCACACCA GTACTTTGCC
    GACGCCAACG AAGCTGAGTC GTGGATGCGC GAAAAGGAAC CGATTGCAAC TGGCAGTGAT
    TATGGTAAGG ACGAGGATTC CTCGGAGGCT TTGCTCAAGA AGCACGAGGC TCTGGTCTCC
    GATCTAGAGG CTTTTGGCAA CACCATTCAG GCGCTGCAGG AGCAGGCCAA GAACTGTCGC
    CAGCAGGAGA CCCCTGTTGT GGATATCACT GGCAAGGAGT GCGTGGTTGC CCTCTACGAC
    TACACGGAGA AATCCCCTCG TGAGGTCTCG ATGAAGAAGG GCGATGTTCT GACCCTGCTG
    AACTCGAACA ACAAAGACTG GTGGAAGGTG GAGGTCAACG ACCGCCAGGG CTTCGTACCG
    GCCGCATACA TCAAGAAGAT TGACGCCGGC TTGAGCGCCT CCCAGCAGAA CCTAGTGGAC
    AATCATTCGA TTGCCAAGCG CCAGAACCAG ATCAACTCGC AGTACGACAA CTTGCTGGCC
    CTCGCCCGCG AGCGCCAGAA CAAGCTAAAC GAGACTGTGA AGGCGTACGT TCTTGTTCGT
    GAGGCTGCCG ACTTGGCCCA GTGGATCCGG GACAAGGAGA ACCACGCTCA GATCGCTGAT
    GTTGTGGGAG AGGATCTGGA AGAGGTGGAG GTGCTCCAAA AGAAGTTCGA CGACTTCAAC
    GACGACCTGA AGGCCAACGA AGTGCGTCTG GCAAACATGA ACGAGATTGC CGTGCAGCTG
    ACTTCCTTGG GTCAGACCGA GGCCGCCCTA AAGATCCAAA CCCAGATGCA AGACCTGAAC
    GAGAAGTGGA ACAACCTGCA GACGCTGACC GCTGAGAAGG CCAGCCAACT GGGATCCGCC
    CATGAGGTGC AACGCTTCCA TCGTGACATT GACGAGACCA AGGATTGGAT CGCTGAGAAG
    GCCAATGCCC TGAACAACGA CGACCTGGGC AAGGATCTGC GCAGTGTCCA GACTCTGCAG
    CGCAAGCACG AGGGCGTGGA ACGCGACTTG GCCGCATTGC GGGACAAGAT CCGCCAGCTG
    GACGAAACCG CCAACAGGCT GATGCAGTCG CACCCGGACA CCGCCGAGCA GACCTACGCC
    AAGCAGAAGG AGATCAACGA GATGTGGGAC CAGATCATCA CCAAGTCCAC CGCCCGCAAG
    GAGAAGCTGC TGGACTCGTA CGACCTGCAG CGTTTCCTCA GCGACTACCG CGATCTGCTG
    GCCTGGATCA ACTCGATGAT GAGCCTGGTC ACGTCCGACG AGCTGGCCAA CGATGTAACA
    GGAGCCGAGG CTCTGATCGA GCGTCATCAG GAACACCGAA CGGAGATCGA TGCGCGCGCC
    GGAACTTTCG GGGCTTTCGA GCAGTTCGGA AACGAGCTGC TGCAGGCCAA CCACTATGCG
    TCGCCTGAAA TCAAGGAGAA AATCGAGGAT CTGGCCAAGG CCCGCGAGGA CTTGGAGAAG
    GCGTGGACAG AGCGCCGCCT GCAGCTGGAG CAGAACTTGG ATCTGCAGCT CTACATGCGT
    GACTGCGAGT TGGCCGAGTC CTGGATGTCA GCCCGCGAGG CCTTCCTAAA TGCCGATGAT
    GATGCCAATG CCGGCGGAAA TGTGGAGGCA CTGATCAAGA AGCACGAGGA CTTCGACAAG
    GCCATTAACG GCCACGAACA GAAGATTGCT GCTCTGCAGA CGGTGGCGGA TCAGTTGATC
    GCCCAGAACC ACTATGCCTC CAATTTGGTG GACGAGAAGC GCAAGCAAGT GCTGGAGCGC
    TGGCGCCATC TCAAGGAGGG TCTGATCGAG AAGCGATCCC GTTTGGGTGA CGAGCAGACG
    CTGCAGCAGT TCTCGCGCGA TGCCGATGAG ATTGAGAACT GGATTGCAGA GAAGTTGCAG
    CTGGCCACCG AAGAGAGCTA CAAGGACCCG GCTAACATTC AGTCGAAGCA CCAGAAGCAC
    CAGGCCTTCG AGGCGGAGCT GGCGGCTAAC GCCGATCGCA TCCAGAGCGT GCTAGCCATG
    GGCGGAAACC TCATCGATAA GAAGCAGTGC AGCGGGTCGG AGGACGCGGT GCAGAAGCGG
    CTGACGCAGA TCGCCGATCA GTGGGAGTAC CTGACCCATA AGACTACCGA GAAGTCGCTG
    AAGCTAAAGG AGGCGAATAA GCAGCGCACC TATATCGCTG CTGTCAAGGA TTTGGACTTC
    TGGTTGGGCG AGGTCGAGAG TCTTTTGACC ACTGAGGATT CTGGTAAGGA TTTGGCCTCT
    GTCCAAAACC TCATGAAGAA GCATCAGTTG GTCGAGGCGG ATATCGTGGC GCACGAAGAC
    CGCATCAAGG ACATGAACAA CCAGGCCGAT TCCTTGGTGG AGAGCGGCCA GTTCGATACT
    GCCGGCATCC AGGAGAAGCG TCAAAGCATC AATGAGCGCT ACGAGCGCAT CTGCAACCTG
    GCGGCCCATC GCCAAGCCCG TCTCAACGAG GCTCTGACCC TGCACCAATT CTTCCGCGAC
    ATCGCCGACG AGGAGAGCTG GATCAAGGAG AAGAAGCTAC TTGTCGGCTC CGACGACTAT
    GGTCGCGATC TGACCGGCGT CCAGAACCTC AAGAAGAAGC ACAAGCGCCT AGAAGCCGAA
    TTGGGCTCTC ACGAGCCGGC CATTCAAGCT GTGCAGGAGG CTGGCGAGAA GCTCATGGAC
    GTGTCCAACC TCGGTGTCCC CGAAATTGAG CAGCGCCTGA AGGCTCTGAA CCAGGCCTGG
    GCCGAGCTAA AGAACCTGGC TGCCACGCGA GGTCAGAAGT TGGATGAGTC GCTGACCTAT
    CAGCAATTCC TCGCTCAGGT GGAGGAGGAG GAGGCCTGGA TCACCGAGAA GCAACAGCTG
    CTCTCTGTGG AGGACTACGG CGACTCGATG GCCGCCGTAC AAGGACTGCT GAAAAAGCAC
    GACGCATTCG AAACGGACTT CACTGCCCAC AAGGACCGCT GTTCTTTGAT CTGCGACCAG
    GGCAGTGAGT TGGTGGAGGC CAAGAACCAC CACGGAGAGT CCATTGCCCA ACGCTGCCAG
    CAGCTGCGCC TGAAACTGGA CAACCTGTCC GCCCTGGCTG CCCGCCGCAA GGGTGCCCTG
    CTGGACAACT CGGCCTATCT GCAGTTCATG TGGAAGGCCG ATGTGGTCGA GAGCTGGATC
    GACGACAAGG AGAACTATGT GCGCTCCGAT GAGTTCGGAC GCGACCTGTC CACCGTGCAA
    ACGCTGTTGA CCAAGCAAGA GACATTCGAT GCAGGTCTCA ATGCCTTCGA GCAGGAGGGC
    ATACACAACA TCACGGCACT TAAGGACCAA CTGATAAATG CCAGTCACGC CCAGTCGCCG
    GCAATCCTAA AGCGTCACGG AGACGTGATT GCCAGGTGGC AGAAGTTGCG CGACGCCTCC
    AACACGCGCA AGGACCGACT GCTGGCCATG CAGGAGCAGT TCCGCCAGAT CGAGGAACTC
    TACTTGACAT TTGCCAAGAA AGCTTCGGCC TTCAATTCTT GGTTCGAAAA TGCCGAGGAG
    GATCTCACAG ATCCTGTTCG CTGCAATTCG ATCGAAGAAA TCCGCGCCCT GCGCGACGCC
    CATGCCCAAT TCCAGGCGTC CCTTTCGTCG GCCGAAGCTG ACTTCAAGGC ATTGGCAGCA
    CTCGACCAGA AGATCAAGAG CTTTAATGTT GGACCCAACC CGTACACGTG GTTCACCATG
    GAAGCTCTTG AGGAGACCTG GCGTAATCTG CAAAAGATCA TAGAGGAACG CGATGGGGAA
    CTTGCCAAGG AGGCCAAGCG GCAGGAGGAG AACGACAAGC TGCGCAAGGA GTTTGCCAAG
    CACGCCAATC TTTTCCACCA GTGGCTAACA GAGACAAGAT ATTATATGCT GGGTTATAAC
    CGTCAAGGAA CGTCCATGAT GGAAGGCTCC GGTTCCCTGG AACAGCAACT GGAGGCGCTT
    CGTGTCAAGG CCACCGAGGT GCGCGCACGT CGCGTTGACC TCAAGAAGAT CGAGGAGCTG
    GGAGCCTTGC TGGAGGAGCA CCTTATTCTG GACAACCGCT ACACGGAACA CTCGACGGTG
    GGCCTGGCCC AGCAGTGGGA TCAGCTTGAC CAGCTGTCTA TGCGCATGCA GCACAACTTA
    GAGCAACAGA TCCAGGCACG CAACCACTCG GGCGTCTCCG AGGACTCACT CAAGGAGTTC
    TCGATGATGT TCAAGCACTT CGATAAGGAC AAGAGCGGCA AGCTCAACCA CCAGGAATTC
    AAGTCCTGCC TGCGCGCCCT CGGCTACGAC CTGCCCATGG TGGAGGAGGG ACAGCCCGAC
    CCGGAGTTCG AAGCAATTCT CGATGTTGTC GACCCCAACC GCGATGGCTA CGTCTCTCTG
    CAGGAATACA TCGCGTTCAT GATCTCCAAG GAGACGGAGA ACGTTCAATC CTACGAGGAG
    ATCGAGAATG CCTTCCGCGC CATCACCGCT GCCGACCGCC CCTATGTAAC TAAGGAGGAG
    CTCTACTGCA ACCTCACCAA GGACATGGCT GACTACTGCG TGCAGCGCAT GAAACCCTTC
    TCGGAACCGC GCTCCGGCCA GCCCATCAAG GATGCCCTGG ACTACATAGA CTTCACGCGA
    ACGCTGTTCC AGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa20438
    Clone ID Related Accession (Same CDS sequence) NM_001274358.1
    Accession Version NM_001274358.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7344bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product alpha spectrin, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGAGAACT TTACACCCAA AGAGGTGAAG ATCCTCGAGA CAGTCGAGGA CATCCAGGAG 
    CGACGTGAGC AGGTTCTGTC CCGCTACAAT GACTTCAAGA TCGAGACGCG CCAGAAGCGT
    GAAAAGCTCG AGGACTCGCG CCGCTTCCAG TACTTCAAGC GCGATGCCGA CGAGTTGGAG
    TCGTGGATCC ACGAGAAGCT GCAGGCAGCC AGCGAGGAGA GCTACCGCGA TCCGACCAAC
    CTGCAGGCCA AAATCCAGAA ACATCAGGCG TTCGAGGCGG AGGTGTCGGC GCACAGCAAC
    GCCATTGTCT CGCTGGACAA CACTGGCCAG GAGATGATCA ACCAGCAACA TTTTGCCTCC
    GAGTCGATTC AGGTCCGCCT CGACGAGCTG CACAAGCTGT GGGAGCTGCT TCTTAGCCGT
    CTGGCCGAGA AGGGTCTAAA GCTGCAGCAG GCTCTCGTCC TGGTGCAGTT CCTGCGCCAG
    TGCGAGGAGG TGATGTTCTG GATTAAGGAC AAGGAGACCT TTGTCACCGC CGACGAGTTC
    GGCCAAGACC TGGAACATGT AGAGGTTCTG CAGCGCAAGT TCGATGAGTT CCAGAAGGAC
    ATGGCCTCCC AGGAGTATCG CGTGACTGAG GTCAACCAGT TGGCCGATAA GTTGGTGCAA
    GATGGTCATC CCGAGCGCGA CACTATTACC AAGCGCAAGG AGGAGCTAAA CGAGGCTTGG
    CAGCGACTTA AGCAGTTAGC CATTGTGCGC CAAGAGAAGC TATTCGGTGC CCACGAGATC
    CAGCGTTTCA ATCGCGATGC CGACGAAACC GTCGCCTGGA TTGCCGAGAA GGATGTGGTC
    CTGTCGTCCG ATGATTATGG CCGCGATTTG GCCAGTGTTC AGGCGCTGCA ACGCAAACAC
    GAAGGAGTGG AGCGGGATTT GGCCGCCTTG GAGGATAAGG TTTCCACACT GGGAGCCGAG
    GCCCAGCGGC TGTGCTCCAT TCACGCCGAT CACAGTGACC AGATCCGTGA CAAACAGGCC
    GAGATTGCCA ACTACTGGCA GAGTCTCACC ACCAAGGCTC GCGAGCGCAA GCAGAAGCTA
    GATGAGTCCT ACTACCTGCA CCGTTTCCTC GCCGATTTCC GCGACCTCGT GTCCTGGATC
    AATGGCATGA AGGCCATCAT CTCGGCCGAT GAGCTGGCTA AGGATGTGGC TGGAGCAGAG
    GCTCTTCTGG AACGTCACCA GGAGCACAAG GGCGAGATCG ACGCTCGTGA GGATAGCTTC
    AAGCTGACCA CTGAATCCGG ACAGAAGCTG CTGGAGCGTG AGCATTACGC CGCCGCCGAA
    ATCCAGGAAA AGCTTGCCGC TCTGGAAAAC GACAAGAGTT CACTGCTCTC GCTGTGGGAA
    GATCGTCGCA TCCTGTACGA GCAGTGCATG GATCTGCAAC TGTTTTACCG CGATACGGAG
    CAGGCTGACA CTTGGATGGC CAAACAGGAG GCCTTCCTGG CCAACGAGGA CCTTGGCGAC
    TCCCTGGACT CTGTGGAGGC GCTAATTAAG AAGCACGAGG ACTTCGAGAA GAGTCTGGCT
    GCCCAGGAGG AGAAGATCAA GGCTTTAGAC ATCTTTGCCA CCAAATTAAT CGATGGTCAG
    CATTATGCCG CCGATGATGT GGCCCAGCGC CGTCAGATGC TCCTAGCCCG GCGGGCTGCT
    CTCCAAGAAA AATCGTCCAA GCGTCGCCAA TTGCTGGAGG ATTCGAACCG CTACCAGCAG
    TTCGAGCGCG ATTGCGACGA GACCAAGGGC TGGATTAGCG AGAAGCTGAA GTTTGCCACC
    GATGACAGCT ATTTGGATCC TACCAACCTT AACGGAAAGA TGCAGAAGCA CCAGAACTTT
    GAGCACGAGC TGAATGCCAA CAAGTCGCGC ATCGAGGACA TCACCAACGT GGGCACCGAG
    CTGATCGAGA AGCAGCACTA TGCTGCCGAC CAGATCAACA CCCGCATGCA GGAGATCGTC
    GTCCTGTGGG AGACCCTCGT CCAGGCTTCC GATAAAAAGG GAACCAAACT CAACGAGGCA
    TGCCAACAGC AGCAGTTCAA CCGCACCATC GAGGACATTG AGCTGTGGCT TAGCGAGATC
    GAGGGTCAAT TGTTGTCGGA GGATCACGGC AAAGACCTGA CCTCCGTTCA GAATTTGCAG
    AAGAAGCACG CCCTGCTGGA GGCCGATGTG ATGGCTCACC AGGATCGCAT TGAAAGCATC
    AAAGTGGCCG CCAACAAGTT CATCGAATCC GGTCACTTTG ACGCGGACAA CATTCGCAAC
    AAGGAGGGCA ATCTGTCGGC TCGCTATGCC GCCCTAGCTG CACCCATGGG CGAGAGGAAA
    CAACACCTTC TTGACTCCCT TCAGGTGCAG CAGCTCTTCC GCGACTTGGA GGACGAGGCT
    GCCTGGATCC GTGAGAAGGA ACCCATTGCC GCATCCACGA ACCGCGGTCG TGACTTGATT
    GGTGTGCAAA ACCTGATCAA GAAACACCAG GCTGTGCTGG CCGAGATCAA CAACCATGAG
    GCCAGGCTGC TGAATGTGAT CAGTTCGGGC GAGAACATGC TGAAGGACCA GCCCTTCGCC
    AGCGACGATA TCCGTCAGCG TTTGGAGGCG CTCCAAGAGC AGTGGAACAC GCTGAAGGAG
    AAGTCCAGCC AGCGAAAGCA GGATCTGGAT GACTCTCTCC AGGCACACCA GTACTTTGCC
    GACGCCAACG AAGCTGAGTC GTGGATGCGC GAAAAGGAAC CGATTGCAAC TGGCAGTGAT
    TATGGTAAGG ACGAGGATTC CTCGGAGGCT TTGCTCAAGA AGCACGAGGC TCTGGTCTCC
    GATCTAGAGG CTTTTGGCAA CACCATTCAG GCGCTGCAGG AGCAGGCCAA GAACTGTCGC
    CAGCAGGAGA CCCCTGTTGT GGATATCACT GGCAAGGAGT GCGTGGTTGC CCTCTACGAC
    TACACGGAGA AATCCCCTCG TGAGGTCTCG ATGAAGAAGG GCGATGTTCT GACCCTGCTG
    AACTCGAACA ACAAAGACTG GTGGAAGGTG GAGGTCAACG ACCGCCAGGG CTTCGTACCG
    GCCGCATACA TCAAGAAGAT TGACGCCGGC TTGAGCGCCT CCCAGCAGAA CCTAGTGGAC
    AATCATTCGA TTGCCAAGCG CCAGAACCAG ATCAACTCGC AGTACGACAA CTTGCTGGCC
    CTCGCCCGCG AGCGCCAGAA CAAGCTAAAC GAGACTGTGA AGGCGTACGT TCTTGTTCGT
    GAGGCTGCCG ACTTGGCCCA GTGGATCCGG GACAAGGAGA ACCACGCTCA GATCGCTGAT
    GTTGTGGGAG AGGATCTGGA AGAGGTGGAG GTGCTCCAAA AGAAGTTCGA CGACTTCAAC
    GACGACCTGA AGGCCAACGA AGTGCGTCTG GCAAACATGA ACGAGATTGC CGTGCAGCTG
    ACTTCCTTGG GTCAGACCGA GGCCGCCCTA AAGATCCAAA CCCAGATGCA AGACCTGAAC
    GAGAAGTGGA ACAACCTGCA GACGCTGACC GCTGAGAAGG CCAGCCAACT GGGATCCGCC
    CATGAGGTGC AACGCTTCCA TCGTGACATT GACGAGACCA AGGATTGGAT CGCTGAGAAG
    GCCAATGCCC TGAACAACGA CGACCTGGGC AAGGATCTGC GCAGTGTCCA GACTCTGCAG
    CGCAAGCACG AGGGCGTGGA ACGCGACTTG GCCGCATTGC GGGACAAGAT CCGCCAGCTG
    GACGAAACCG CCAACAGGCT GATGCAGTCG CACCCGGACA CCGCCGAGCA GACCTACGCC
    AAGCAGAAGG AGATCAACGA GATGTGGGAC CAGATCATCA CCAAGTCCAC CGCCCGCAAG
    GAGAAGCTGC TGGACTCGTA CGACCTGCAG CGTTTCCTCA GCGACTACCG CGATCTGCTG
    GCCTGGATCA ACTCGATGAT GAGCCTGGTC ACGTCCGACG AGCTGGCCAA CGATGTAACA
    GGAGCCGAGG CTCTGATCGA GCGTCATCAG GCACGCCGTG CCGAGATTGG GTTCACATTG
    GGCATCAGCT CGGCTCCCGG AGCCGCCGCC TCGACCTCCA GCATCGCATC TCCATCCGGC
    GACGAGGAAC ACCGAACGGA GATCGATGCG CGCGCCGGAA CTTTCGGGGC TTTCGAGCAG
    TTCGGAAACG AGCTGCTGCA GGCCAACCAC TATGCGTCGC CTGAAATCAA GGAGAAAATC
    GAGGATCTGG CCAAGGCCCG CGAGGACTTG GAGAAGGCGT GGACAGAGCG CCGCCTGCAG
    CTGGAGCAGA ACTTGGATCT GCAGCTCTAC ATGCGTGACT GCGAGTTGGC CGAGTCCTGG
    ATGTCAGCCC GCGAGGCCTT CCTAAATGCC GATGATGATG CCAATGCCGG CGGAAATGTG
    GAGGCACTGA TCAAGAAGCA CGAGGACTTC GACAAGGCCA TTAACGGCCA CGAACAGAAG
    ATTGCTGCTC TGCAGACGGT GGCGGATCAG TTGATCGCCC AGAACCACTA TGCCTCCAAT
    TTGGTGGACG AGAAGCGCAA GCAAGTGCTG GAGCGCTGGC GCCATCTCAA GGAGGGTCTG
    ATCGAGAAGC GATCCCGTTT GGGTGACGAG CAGACGCTGC AGCAGTTCTC GCGCGATGCC
    GATGAGATTG AGAACTGGAT TGCAGAGAAG TTGCAGCTGG CCACCGAAGA GAGCTACAAG
    GACCCGGCTA ACATTCAGTC GAAGCACCAG AAGCACCAGG CCTTCGAGGC GGAGCTGGCG
    GCTAACGCCG ATCGCATCCA GAGCGTGCTA GCCATGGGCG GAAACCTCAT CGATAAGAAG
    CAGTGCAGCG GGTCGGAGGA CGCGGTGCAG AAGCGGCTGA CGCAGATCGC CGATCAGTGG
    GAGTACCTGA CCCATAAGAC TACCGAGAAG TCGCTGAAGC TAAAGGAGGC GAATAAGCAG
    CGCACCTATA TCGCTGCTGT CAAGGATTTG GACTTCTGGT TGGGCGAGGT CGAGAGTCTT
    TTGACCACTG AGGATTCTGG TAAGGATTTG GCCTCTGTCC AAAACCTCAT GAAGAAGCAT
    CAGTTGGTCG AGGCGGATAT CGTGGCGCAC GAAGACCGCA TCAAGGACAT GAACAACCAG
    GCCGATTCCT TGGTGGAGAG CGGCCAGTTC GATACTGCCG GCATCCAGGA GAAGCGTCAA
    AGCATCAATG AGCGCTACGA GCGCATCTGC AACCTGGCGG CCCATCGCCA AGCCCGTCTC
    AACGAGGCTC TGACCCTGCA CCAATTCTTC CGCGACATCG CCGACGAGGA GAGCTGGATC
    AAGGAGAAGA AGCTACTTGT CGGCTCCGAC GACTATGGTC GCGATCTGAC CGGCGTCCAG
    AACCTCAAGA AGAAGCACAA GCGCCTAGAA GCCGAATTGG GCTCTCACGA GCCGGCCATT
    CAAGCTGTGC AGGAGGCTGG CGAGAAGCTC ATGGACGTGT CCAACCTCGG TGTCCCCGAA
    ATTGAGCAGC GCCTGAAGGC TCTGAACCAG GCCTGGGCCG AGCTAAAGAA CCTGGCTGCC
    ACGCGAGGTC AGAAGTTGGA TGAGTCGCTG ACCTATCAGC AATTCCTCGC TCAGGTGGAG
    GAGGAGGAGG CCTGGATCAC CGAGAAGCAA CAGCTGCTCT CTGTGGAGGA CTACGGCGAC
    TCGATGGCCG CCGTACAAGG ACTGCTGAAA AAGCACGACG CATTCGAAAC GGACTTCACT
    GCCCACAAGG ACCGCTGTTC TTTGATCTGC GACCAGGGCA GTGAGTTGGT GGAGGCCAAG
    AACCACCACG GAGAGTCCAT TGCCCAACGC TGCCAGCAGC TGCGCCTGAA ACTGGACAAC
    CTGTCCGCCC TGGCTGCCCG CCGCAAGGGT GCCCTGCTGG ACAACTCGGC CTATCTGCAG
    TTCATGTGGA AGGCCGATGT GGTCGAGAGC TGGATCGACG ACAAGGAGAA CTATGTGCGC
    TCCGATGAGT TCGGACGCGA CCTGTCCACC GTGCAAACGC TGTTGACCAA GCAAGAGACA
    TTCGATGCAG GTCTCAATGC CTTCGAGCAG GAGGGCATAC ACAACATCAC GGCACTTAAG
    GACCAACTGA TAAATGCCAG TCACGCCCAG TCGCCGGCAA TCCTAAAGCG TCACGGAGAC
    GTGATTGCCA GGTGGCAGAA GTTGCGCGAC GCCTCCAACA CGCGCAAGGA CCGACTGCTG
    GCCATGCAGG AGCAGTTCCG CCAGATCGAG GAACTCTACT TGACATTTGC CAAGAAAGCT
    TCGGCCTTCA ATTCTTGGTT CGAAAATGCC GAGGAGGATC TCACAGATCC TGTTCGCTGC
    AATTCGATCG AAGAAATCCG CGCCCTGCGC GACGCCCATG CCCAATTCCA GGCGTCCCTT
    TCGTCGGCCG AAGCTGACTT CAAGGCATTG GCAGCACTCG ACCAGAAGAT CAAGAGCTTT
    AATGTTGGAC CCAACCCGTA CACGTGGTTC ACCATGGAAG CTCTTGAGGA GACCTGGCGT
    AATCTGCAAA AGATCATAGA GGAACGCGAT GGGGAACTTG CCAAGGAGGC CAAGCGGCAG
    GAGGAGAACG ACAAGCTGCG CAAGGAGTTT GCCAAGCACG CCAATCTTTT CCACCAGTGG
    CTAACAGAGA CAAGAACGTC CATGATGGAA GGCTCCGGTT CCCTGGAACA GCAACTGGAG
    GCGCTTCGTG TCAAGGCCAC CGAGGTGCGC GCACGTCGCG TTGACCTCAA GAAGATCGAG
    GAGCTGGGAG CCTTGCTGGA GGAGCACCTT ATTCTGGACA ACCGCTACAC GGAACACTCG
    ACGGTGGGCC TGGCCCAGCA GTGGGATCAG CTTGACCAGC TGTCTATGCG CATGCAGCAC
    AACTTAGAGC AACAGATCCA GGCACGCAAC CACTCGGGCG TCTCCGAGGA CTCACTCAAG
    GAGTTCTCGA TGATGTTCAA GCACTTCGAT AAGGACAAGA GCGGCAAGCT CAACCACCAG
    GAATTCAAGT CCTGCCTGCG CGCCCTCGGC TACGACCTGC CCATGGTGGA GGAGGGACAG
    CCCGACCCGG AGTTCGAAGC AATTCTCGAT GTTGTCGACC CCAACCGCGA TGGCTACGTC
    TCTCTGCAGG AATACATCGC GTTCATGATC TCCAAGGAGA CGGAGAACGT TCAATCCTAC
    GAGGAGATCG AGAATGCCTT CCGCGCCATC ACCGCTGCCG ACCGCCCCTA TGTAACTAAG
    GAGGAGCTCT ACTGCAACCT CACCAAGGAC ATGGCTGACT ACTGCGTGCA GCGCATGAAA
    CCCTTCTCGG AACCGCGCTC CGGCCAGCCC ATCAAGGATG CCCTGGACTA CATAGACTTC
    ACGCGAACGC TGTTCCAGAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001261287.1
    CDS277..7620
    Translation

    Target ORF information:

    RefSeq Version NM_001274358.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster alpha spectrin, transcript variant C (alpha-Spec), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001274358.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGAGAACT TTACACCCAA AGAGGTGAAG ATCCTCGAGA CAGTCGAGGA CATCCAGGAG 
    CGACGTGAGC AGGTTCTGTC CCGCTACAAT GACTTCAAGA TCGAGACGCG CCAGAAGCGT
    GAAAAGCTCG AGGACTCGCG CCGCTTCCAG TACTTCAAGC GCGATGCCGA CGAGTTGGAG
    TCGTGGATCC ACGAGAAGCT GCAGGCAGCC AGCGAGGAGA GCTACCGCGA TCCGACCAAC
    CTGCAGGCCA AAATCCAGAA ACATCAGGCG TTCGAGGCGG AGGTGTCGGC GCACAGCAAC
    GCCATTGTCT CGCTGGACAA CACTGGCCAG GAGATGATCA ACCAGCAACA TTTTGCCTCC
    GAGTCGATTC AGGTCCGCCT CGACGAGCTG CACAAGCTGT GGGAGCTGCT TCTTAGCCGT
    CTGGCCGAGA AGGGTCTAAA GCTGCAGCAG GCTCTCGTCC TGGTGCAGTT CCTGCGCCAG
    TGCGAGGAGG TGATGTTCTG GATTAAGGAC AAGGAGACCT TTGTCACCGC CGACGAGTTC
    GGCCAAGACC TGGAACATGT AGAGGTTCTG CAGCGCAAGT TCGATGAGTT CCAGAAGGAC
    ATGGCCTCCC AGGAGTATCG CGTGACTGAG GTCAACCAGT TGGCCGATAA GTTGGTGCAA
    GATGGTCATC CCGAGCGCGA CACTATTACC AAGCGCAAGG AGGAGCTAAA CGAGGCTTGG
    CAGCGACTTA AGCAGTTAGC CATTGTGCGC CAAGAGAAGC TATTCGGTGC CCACGAGATC
    CAGCGTTTCA ATCGCGATGC CGACGAAACC GTCGCCTGGA TTGCCGAGAA GGATGTGGTC
    CTGTCGTCCG ATGATTATGG CCGCGATTTG GCCAGTGTTC AGGCGCTGCA ACGCAAACAC
    GAAGGAGTGG AGCGGGATTT GGCCGCCTTG GAGGATAAGG TTTCCACACT GGGAGCCGAG
    GCCCAGCGGC TGTGCTCCAT TCACGCCGAT CACAGTGACC AGATCCGTGA CAAACAGGCC
    GAGATTGCCA ACTACTGGCA GAGTCTCACC ACCAAGGCTC GCGAGCGCAA GCAGAAGCTA
    GATGAGTCCT ACTACCTGCA CCGTTTCCTC GCCGATTTCC GCGACCTCGT GTCCTGGATC
    AATGGCATGA AGGCCATCAT CTCGGCCGAT GAGCTGGCTA AGGATGTGGC TGGAGCAGAG
    GCTCTTCTGG AACGTCACCA GGAGCACAAG GGCGAGATCG ACGCTCGTGA GGATAGCTTC
    AAGCTGACCA CTGAATCCGG ACAGAAGCTG CTGGAGCGTG AGCATTACGC CGCCGCCGAA
    ATCCAGGAAA AGCTTGCCGC TCTGGAAAAC GACAAGAGTT CACTGCTCTC GCTGTGGGAA
    GATCGTCGCA TCCTGTACGA GCAGTGCATG GATCTGCAAC TGTTTTACCG CGATACGGAG
    CAGGCTGACA CTTGGATGGC CAAACAGGAG GCCTTCCTGG CCAACGAGGA CCTTGGCGAC
    TCCCTGGACT CTGTGGAGGC GCTAATTAAG AAGCACGAGG ACTTCGAGAA GAGTCTGGCT
    GCCCAGGAGG AGAAGATCAA GGCTTTAGAC ATCTTTGCCA CCAAATTAAT CGATGGTCAG
    CATTATGCCG CCGATGATGT GGCCCAGCGC CGTCAGATGC TCCTAGCCCG GCGGGCTGCT
    CTCCAAGAAA AATCGTCCAA GCGTCGCCAA TTGCTGGAGG ATTCGAACCG CTACCAGCAG
    TTCGAGCGCG ATTGCGACGA GACCAAGGGC TGGATTAGCG AGAAGCTGAA GTTTGCCACC
    GATGACAGCT ATTTGGATCC TACCAACCTT AACGGAAAGA TGCAGAAGCA CCAGAACTTT
    GAGCACGAGC TGAATGCCAA CAAGTCGCGC ATCGAGGACA TCACCAACGT GGGCACCGAG
    CTGATCGAGA AGCAGCACTA TGCTGCCGAC CAGATCAACA CCCGCATGCA GGAGATCGTC
    GTCCTGTGGG AGACCCTCGT CCAGGCTTCC GATAAAAAGG GAACCAAACT CAACGAGGCA
    TGCCAACAGC AGCAGTTCAA CCGCACCATC GAGGACATTG AGCTGTGGCT TAGCGAGATC
    GAGGGTCAAT TGTTGTCGGA GGATCACGGC AAAGACCTGA CCTCCGTTCA GAATTTGCAG
    AAGAAGCACG CCCTGCTGGA GGCCGATGTG ATGGCTCACC AGGATCGCAT TGAAAGCATC
    AAAGTGGCCG CCAACAAGTT CATCGAATCC GGTCACTTTG ACGCGGACAA CATTCGCAAC
    AAGGAGGGCA ATCTGTCGGC TCGCTATGCC GCCCTAGCTG CACCCATGGG CGAGAGGAAA
    CAACACCTTC TTGACTCCCT TCAGGTGCAG CAGCTCTTCC GCGACTTGGA GGACGAGGCT
    GCCTGGATCC GTGAGAAGGA ACCCATTGCC GCATCCACGA ACCGCGGTCG TGACTTGATT
    GGTGTGCAAA ACCTGATCAA GAAACACCAG GCTGTGCTGG CCGAGATCAA CAACCATGAG
    GCCAGGCTGC TGAATGTGAT CAGTTCGGGC GAGAACATGC TGAAGGACCA GCCCTTCGCC
    AGCGACGATA TCCGTCAGCG TTTGGAGGCG CTCCAAGAGC AGTGGAACAC GCTGAAGGAG
    AAGTCCAGCC AGCGAAAGCA GGATCTGGAT GACTCTCTCC AGGCACACCA GTACTTTGCC
    GACGCCAACG AAGCTGAGTC GTGGATGCGC GAAAAGGAAC CGATTGCAAC TGGCAGTGAT
    TATGGTAAGG ACGAGGATTC CTCGGAGGCT TTGCTCAAGA AGCACGAGGC TCTGGTCTCC
    GATCTAGAGG CTTTTGGCAA CACCATTCAG GCGCTGCAGG AGCAGGCCAA GAACTGTCGC
    CAGCAGGAGA CCCCTGTTGT GGATATCACT GGCAAGGAGT GCGTGGTTGC CCTCTACGAC
    TACACGGAGA AATCCCCTCG TGAGGTCTCG ATGAAGAAGG GCGATGTTCT GACCCTGCTG
    AACTCGAACA ACAAAGACTG GTGGAAGGTG GAGGTCAACG ACCGCCAGGG CTTCGTACCG
    GCCGCATACA TCAAGAAGAT TGACGCCGGC TTGAGCGCCT CCCAGCAGAA CCTAGTGGAC
    AATCATTCGA TTGCCAAGCG CCAGAACCAG ATCAACTCGC AGTACGACAA CTTGCTGGCC
    CTCGCCCGCG AGCGCCAGAA CAAGCTAAAC GAGACTGTGA AGGCGTACGT TCTTGTTCGT
    GAGGCTGCCG ACTTGGCCCA GTGGATCCGG GACAAGGAGA ACCACGCTCA GATCGCTGAT
    GTTGTGGGAG AGGATCTGGA AGAGGTGGAG GTGCTCCAAA AGAAGTTCGA CGACTTCAAC
    GACGACCTGA AGGCCAACGA AGTGCGTCTG GCAAACATGA ACGAGATTGC CGTGCAGCTG
    ACTTCCTTGG GTCAGACCGA GGCCGCCCTA AAGATCCAAA CCCAGATGCA AGACCTGAAC
    GAGAAGTGGA ACAACCTGCA GACGCTGACC GCTGAGAAGG CCAGCCAACT GGGATCCGCC
    CATGAGGTGC AACGCTTCCA TCGTGACATT GACGAGACCA AGGATTGGAT CGCTGAGAAG
    GCCAATGCCC TGAACAACGA CGACCTGGGC AAGGATCTGC GCAGTGTCCA GACTCTGCAG
    CGCAAGCACG AGGGCGTGGA ACGCGACTTG GCCGCATTGC GGGACAAGAT CCGCCAGCTG
    GACGAAACCG CCAACAGGCT GATGCAGTCG CACCCGGACA CCGCCGAGCA GACCTACGCC
    AAGCAGAAGG AGATCAACGA GATGTGGGAC CAGATCATCA CCAAGTCCAC CGCCCGCAAG
    GAGAAGCTGC TGGACTCGTA CGACCTGCAG CGTTTCCTCA GCGACTACCG CGATCTGCTG
    GCCTGGATCA ACTCGATGAT GAGCCTGGTC ACGTCCGACG AGCTGGCCAA CGATGTAACA
    GGAGCCGAGG CTCTGATCGA GCGTCATCAG GCACGCCGTG CCGAGATTGG GTTCACATTG
    GGCATCAGCT CGGCTCCCGG AGCCGCCGCC TCGACCTCCA GCATCGCATC TCCATCCGGC
    GACGAGGAAC ACCGAACGGA GATCGATGCG CGCGCCGGAA CTTTCGGGGC TTTCGAGCAG
    TTCGGAAACG AGCTGCTGCA GGCCAACCAC TATGCGTCGC CTGAAATCAA GGAGAAAATC
    GAGGATCTGG CCAAGGCCCG CGAGGACTTG GAGAAGGCGT GGACAGAGCG CCGCCTGCAG
    CTGGAGCAGA ACTTGGATCT GCAGCTCTAC ATGCGTGACT GCGAGTTGGC CGAGTCCTGG
    ATGTCAGCCC GCGAGGCCTT CCTAAATGCC GATGATGATG CCAATGCCGG CGGAAATGTG
    GAGGCACTGA TCAAGAAGCA CGAGGACTTC GACAAGGCCA TTAACGGCCA CGAACAGAAG
    ATTGCTGCTC TGCAGACGGT GGCGGATCAG TTGATCGCCC AGAACCACTA TGCCTCCAAT
    TTGGTGGACG AGAAGCGCAA GCAAGTGCTG GAGCGCTGGC GCCATCTCAA GGAGGGTCTG
    ATCGAGAAGC GATCCCGTTT GGGTGACGAG CAGACGCTGC AGCAGTTCTC GCGCGATGCC
    GATGAGATTG AGAACTGGAT TGCAGAGAAG TTGCAGCTGG CCACCGAAGA GAGCTACAAG
    GACCCGGCTA ACATTCAGTC GAAGCACCAG AAGCACCAGG CCTTCGAGGC GGAGCTGGCG
    GCTAACGCCG ATCGCATCCA GAGCGTGCTA GCCATGGGCG GAAACCTCAT CGATAAGAAG
    CAGTGCAGCG GGTCGGAGGA CGCGGTGCAG AAGCGGCTGA CGCAGATCGC CGATCAGTGG
    GAGTACCTGA CCCATAAGAC TACCGAGAAG TCGCTGAAGC TAAAGGAGGC GAATAAGCAG
    CGCACCTATA TCGCTGCTGT CAAGGATTTG GACTTCTGGT TGGGCGAGGT CGAGAGTCTT
    TTGACCACTG AGGATTCTGG TAAGGATTTG GCCTCTGTCC AAAACCTCAT GAAGAAGCAT
    CAGTTGGTCG AGGCGGATAT CGTGGCGCAC GAAGACCGCA TCAAGGACAT GAACAACCAG
    GCCGATTCCT TGGTGGAGAG CGGCCAGTTC GATACTGCCG GCATCCAGGA GAAGCGTCAA
    AGCATCAATG AGCGCTACGA GCGCATCTGC AACCTGGCGG CCCATCGCCA AGCCCGTCTC
    AACGAGGCTC TGACCCTGCA CCAATTCTTC CGCGACATCG CCGACGAGGA GAGCTGGATC
    AAGGAGAAGA AGCTACTTGT CGGCTCCGAC GACTATGGTC GCGATCTGAC CGGCGTCCAG
    AACCTCAAGA AGAAGCACAA GCGCCTAGAA GCCGAATTGG GCTCTCACGA GCCGGCCATT
    CAAGCTGTGC AGGAGGCTGG CGAGAAGCTC ATGGACGTGT CCAACCTCGG TGTCCCCGAA
    ATTGAGCAGC GCCTGAAGGC TCTGAACCAG GCCTGGGCCG AGCTAAAGAA CCTGGCTGCC
    ACGCGAGGTC AGAAGTTGGA TGAGTCGCTG ACCTATCAGC AATTCCTCGC TCAGGTGGAG
    GAGGAGGAGG CCTGGATCAC CGAGAAGCAA CAGCTGCTCT CTGTGGAGGA CTACGGCGAC
    TCGATGGCCG CCGTACAAGG ACTGCTGAAA AAGCACGACG CATTCGAAAC GGACTTCACT
    GCCCACAAGG ACCGCTGTTC TTTGATCTGC GACCAGGGCA GTGAGTTGGT GGAGGCCAAG
    AACCACCACG GAGAGTCCAT TGCCCAACGC TGCCAGCAGC TGCGCCTGAA ACTGGACAAC
    CTGTCCGCCC TGGCTGCCCG CCGCAAGGGT GCCCTGCTGG ACAACTCGGC CTATCTGCAG
    TTCATGTGGA AGGCCGATGT GGTCGAGAGC TGGATCGACG ACAAGGAGAA CTATGTGCGC
    TCCGATGAGT TCGGACGCGA CCTGTCCACC GTGCAAACGC TGTTGACCAA GCAAGAGACA
    TTCGATGCAG GTCTCAATGC CTTCGAGCAG GAGGGCATAC ACAACATCAC GGCACTTAAG
    GACCAACTGA TAAATGCCAG TCACGCCCAG TCGCCGGCAA TCCTAAAGCG TCACGGAGAC
    GTGATTGCCA GGTGGCAGAA GTTGCGCGAC GCCTCCAACA CGCGCAAGGA CCGACTGCTG
    GCCATGCAGG AGCAGTTCCG CCAGATCGAG GAACTCTACT TGACATTTGC CAAGAAAGCT
    TCGGCCTTCA ATTCTTGGTT CGAAAATGCC GAGGAGGATC TCACAGATCC TGTTCGCTGC
    AATTCGATCG AAGAAATCCG CGCCCTGCGC GACGCCCATG CCCAATTCCA GGCGTCCCTT
    TCGTCGGCCG AAGCTGACTT CAAGGCATTG GCAGCACTCG ACCAGAAGAT CAAGAGCTTT
    AATGTTGGAC CCAACCCGTA CACGTGGTTC ACCATGGAAG CTCTTGAGGA GACCTGGCGT
    AATCTGCAAA AGATCATAGA GGAACGCGAT GGGGAACTTG CCAAGGAGGC CAAGCGGCAG
    GAGGAGAACG ACAAGCTGCG CAAGGAGTTT GCCAAGCACG CCAATCTTTT CCACCAGTGG
    CTAACAGAGA CAAGAACGTC CATGATGGAA GGCTCCGGTT CCCTGGAACA GCAACTGGAG
    GCGCTTCGTG TCAAGGCCAC CGAGGTGCGC GCACGTCGCG TTGACCTCAA GAAGATCGAG
    GAGCTGGGAG CCTTGCTGGA GGAGCACCTT ATTCTGGACA ACCGCTACAC GGAACACTCG
    ACGGTGGGCC TGGCCCAGCA GTGGGATCAG CTTGACCAGC TGTCTATGCG CATGCAGCAC
    AACTTAGAGC AACAGATCCA GGCACGCAAC CACTCGGGCG TCTCCGAGGA CTCACTCAAG
    GAGTTCTCGA TGATGTTCAA GCACTTCGAT AAGGACAAGA GCGGCAAGCT CAACCACCAG
    GAATTCAAGT CCTGCCTGCG CGCCCTCGGC TACGACCTGC CCATGGTGGA GGAGGGACAG
    CCCGACCCGG AGTTCGAAGC AATTCTCGAT GTTGTCGACC CCAACCGCGA TGGCTACGTC
    TCTCTGCAGG AATACATCGC GTTCATGATC TCCAAGGAGA CGGAGAACGT TCAATCCTAC
    GAGGAGATCG AGAATGCCTT CCGCGCCATC ACCGCTGCCG ACCGCCCCTA TGTAACTAAG
    GAGGAGCTCT ACTGCAACCT CACCAAGGAC ATGGCTGACT ACTGCGTGCA GCGCATGAAA
    CCCTTCTCGG AACCGCGCTC CGGCCAGCCC ATCAAGGATG CCCTGGACTA CATAGACTTC
    ACGCGAACGC TGTTCCAGAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa20439
    Clone ID Related Accession (Same CDS sequence) NM_001274357.1
    Accession Version NM_001274357.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7374bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product alpha spectrin, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGAGAACT TTACACCCAA AGAGGTGAAG ATCCTCGAGA CAGTCGAGGA CATCCAGGAG 
    CGACGTGAGC AGGTTCTGTC CCGCTACAAT GACTTCAAGA TCGAGACGCG CCAGAAGCGT
    GAAAAGCTCG AGGACTCGCG CCGCTTCCAG TACTTCAAGC GCGATGCCGA CGAGTTGGAG
    TCGTGGATCC ACGAGAAGCT GCAGGCAGCC AGCGAGGAGA GCTACCGCGA TCCGACCAAC
    CTGCAGGCCA AAATCCAGAA ACATCAGGCG TTCGAGGCGG AGGTGTCGGC GCACAGCAAC
    GCCATTGTCT CGCTGGACAA CACTGGCCAG GAGATGATCA ACCAGCAACA TTTTGCCTCC
    GAGTCGATTC AGGTCCGCCT CGACGAGCTG CACAAGCTGT GGGAGCTGCT TCTTAGCCGT
    CTGGCCGAGA AGGGTCTAAA GCTGCAGCAG GCTCTCGTCC TGGTGCAGTT CCTGCGCCAG
    TGCGAGGAGG TGATGTTCTG GATTAAGGAC AAGGAGACCT TTGTCACCGC CGACGAGTTC
    GGCCAAGACC TGGAACATGT AGAGGTTCTG CAGCGCAAGT TCGATGAGTT CCAGAAGGAC
    ATGGCCTCCC AGGAGTATCG CGTGACTGAG GTCAACCAGT TGGCCGATAA GTTGGTGCAA
    GATGGTCATC CCGAGCGCGA CACTATTACC AAGCGCAAGG AGGAGCTAAA CGAGGCTTGG
    CAGCGACTTA AGCAGTTAGC CATTGTGCGC CAAGAGAAGC TATTCGGTGC CCACGAGATC
    CAGCGTTTCA ATCGCGATGC CGACGAAACC GTCGCCTGGA TTGCCGAGAA GGATGTGGTC
    CTGTCGTCCG ATGATTATGG CCGCGATTTG GCCAGTGTTC AGGCGCTGCA ACGCAAACAC
    GAAGGAGTGG AGCGGGATTT GGCCGCCTTG GAGGATAAGG TTTCCACACT GGGAGCCGAG
    GCCCAGCGGC TGTGCTCCAT TCACGCCGAT CACAGTGACC AGATCCGTGA CAAACAGGCC
    GAGATTGCCA ACTACTGGCA GAGTCTCACC ACCAAGGCTC GCGAGCGCAA GCAGAAGCTA
    GATGAGTCCT ACTACCTGCA CCGTTTCCTC GCCGATTTCC GCGACCTCGT GTCCTGGATC
    AATGGCATGA AGGCCATCAT CTCGGCCGAT GAGCTGGCTA AGGATGTGGC TGGAGCAGAG
    GCTCTTCTGG AACGTCACCA GGAGCACAAG GGCGAGATCG ACGCTCGTGA GGATAGCTTC
    AAGCTGACCA CTGAATCCGG ACAGAAGCTG CTGGAGCGTG AGCATTACGC CGCCGCCGAA
    ATCCAGGAAA AGCTTGCCGC TCTGGAAAAC GACAAGAGTT CACTGCTCTC GCTGTGGGAA
    GATCGTCGCA TCCTGTACGA GCAGTGCATG GATCTGCAAC TGTTTTACCG CGATACGGAG
    CAGGCTGACA CTTGGATGGC CAAACAGGAG GCCTTCCTGG CCAACGAGGA CCTTGGCGAC
    TCCCTGGACT CTGTGGAGGC GCTAATTAAG AAGCACGAGG ACTTCGAGAA GAGTCTGGCT
    GCCCAGGAGG AGAAGATCAA GGCTTTAGAC ATCTTTGCCA CCAAATTAAT CGATGGTCAG
    CATTATGCCG CCGATGATGT GGCCCAGCGC CGTCAGATGC TCCTAGCCCG GCGGGCTGCT
    CTCCAAGAAA AATCGTCCAA GCGTCGCCAA TTGCTGGAGG ATTCGAACCG CTACCAGCAG
    TTCGAGCGCG ATTGCGACGA GACCAAGGGC TGGATTAGCG AGAAGCTGAA GTTTGCCACC
    GATGACAGCT ATTTGGATCC TACCAACCTT AACGGAAAGA TGCAGAAGCA CCAGAACTTT
    GAGCACGAGC TGAATGCCAA CAAGTCGCGC ATCGAGGACA TCACCAACGT GGGCACCGAG
    CTGATCGAGA AGCAGCACTA TGCTGCCGAC CAGATCAACA CCCGCATGCA GGAGATCGTC
    GTCCTGTGGG AGACCCTCGT CCAGGCTTCC GATAAAAAGG GAACCAAACT CAACGAGGCA
    TGCCAACAGC AGCAGTTCAA CCGCACCATC GAGGACATTG AGCTGTGGCT TAGCGAGATC
    GAGGGTCAAT TGTTGTCGGA GGATCACGGC AAAGACCTGA CCTCCGTTCA GAATTTGCAG
    AAGAAGCACG CCCTGCTGGA GGCCGATGTG ATGGCTCACC AGGATCGCAT TGAAAGCATC
    AAAGTGGCCG CCAACAAGTT CATCGAATCC GGTCACTTTG ACGCGGACAA CATTCGCAAC
    AAGGAGGGCA ATCTGTCGGC TCGCTATGCC GCCCTAGCTG CACCCATGGG CGAGAGGAAA
    CAACACCTTC TTGACTCCCT TCAGGTGCAG CAGCTCTTCC GCGACTTGGA GGACGAGGCT
    GCCTGGATCC GTGAGAAGGA ACCCATTGCC GCATCCACGA ACCGCGGTCG TGACTTGATT
    GGTGTGCAAA ACCTGATCAA GAAACACCAG GCTGTGCTGG CCGAGATCAA CAACCATGAG
    GCCAGGCTGC TGAATGTGAT CAGTTCGGGC GAGAACATGC TGAAGGACCA GCCCTTCGCC
    AGCGACGATA TCCGTCAGCG TTTGGAGGCG CTCCAAGAGC AGTGGAACAC GCTGAAGGAG
    AAGTCCAGCC AGCGAAAGCA GGATCTGGAT GACTCTCTCC AGGCACACCA GTACTTTGCC
    GACGCCAACG AAGCTGAGTC GTGGATGCGC GAAAAGGAAC CGATTGCAAC TGGCAGTGAT
    TATGGTAAGG ACGAGGATTC CTCGGAGGCT TTGCTCAAGA AGCACGAGGC TCTGGTCTCC
    GATCTAGAGG CTTTTGGCAA CACCATTCAG GCGCTGCAGG AGCAGGCCAA GAACTGTCGC
    CAGCAGGAGA CCCCTGTTGT GGATATCACT GGCAAGGAGT GCGTGGTTGC CCTCTACGAC
    TACACGGAGA AATCCCCTCG TGAGGTCTCG ATGAAGAAGG GCGATGTTCT GACCCTGCTG
    AACTCGAACA ACAAAGACTG GTGGAAGGTG GAGGTCAACG ACCGCCAGGG CTTCGTACCG
    GCCGCATACA TCAAGAAGAT TGACGCCGGC TTGAGCGCCT CCCAGCAGAA CCTAGTGGAC
    AATCATTCGA TTGCCAAGCG CCAGAACCAG ATCAACTCGC AGTACGACAA CTTGCTGGCC
    CTCGCCCGCG AGCGCCAGAA CAAGCTAAAC GAGACTGTGA AGGCGTACGT TCTTGTTCGT
    GAGGCTGCCG ACTTGGCCCA GTGGATCCGG GACAAGGAGA ACCACGCTCA GATCGCTGAT
    GTTGTGGGAG AGGATCTGGA AGAGGTGGAG GTGCTCCAAA AGAAGTTCGA CGACTTCAAC
    GACGACCTGA AGGCCAACGA AGTGCGTCTG GCAAACATGA ACGAGATTGC CGTGCAGCTG
    ACTTCCTTGG GTCAGACCGA GGCCGCCCTA AAGATCCAAA CCCAGATGCA AGACCTGAAC
    GAGAAGTGGA ACAACCTGCA GACGCTGACC GCTGAGAAGG CCAGCCAACT GGGATCCGCC
    CATGAGGTGC AACGCTTCCA TCGTGACATT GACGAGACCA AGGATTGGAT CGCTGAGAAG
    GCCAATGCCC TGAACAACGA CGACCTGGGC AAGGATCTGC GCAGTGTCCA GACTCTGCAG
    CGCAAGCACG AGGGCGTGGA ACGCGACTTG GCCGCATTGC GGGACAAGAT CCGCCAGCTG
    GACGAAACCG CCAACAGGCT GATGCAGTCG CACCCGGACA CCGCCGAGCA GACCTACGCC
    AAGCAGAAGG AGATCAACGA GATGTGGGAC CAGATCATCA CCAAGTCCAC CGCCCGCAAG
    GAGAAGCTGC TGGACTCGTA CGACCTGCAG CGTTTCCTCA GCGACTACCG CGATCTGCTG
    GCCTGGATCA ACTCGATGAT GAGCCTGGTC ACGTCCGACG AGCTGGCCAA CGATGTAACA
    GGAGCCGAGG CTCTGATCGA GCGTCATCAG GCACGCCGTG CCGAGATTGG GTTCACATTG
    GGCATCAGCT CGGCTCCCGG AGCCGCCGCC TCGACCTCCA GCATCGCATC TCCATCCGGC
    GACGAGGAAC ACCGAACGGA GATCGATGCG CGCGCCGGAA CTTTCGGGGC TTTCGAGCAG
    TTCGGAAACG AGCTGCTGCA GGCCAACCAC TATGCGTCGC CTGAAATCAA GGAGAAAATC
    GAGGATCTGG CCAAGGCCCG CGAGGACTTG GAGAAGGCGT GGACAGAGCG CCGCCTGCAG
    CTGGAGCAGA ACTTGGATCT GCAGCTCTAC ATGCGTGACT GCGAGTTGGC CGAGTCCTGG
    ATGTCAGCCC GCGAGGCCTT CCTAAATGCC GATGATGATG CCAATGCCGG CGGAAATGTG
    GAGGCACTGA TCAAGAAGCA CGAGGACTTC GACAAGGCCA TTAACGGCCA CGAACAGAAG
    ATTGCTGCTC TGCAGACGGT GGCGGATCAG TTGATCGCCC AGAACCACTA TGCCTCCAAT
    TTGGTGGACG AGAAGCGCAA GCAAGTGCTG GAGCGCTGGC GCCATCTCAA GGAGGGTCTG
    ATCGAGAAGC GATCCCGTTT GGGTGACGAG CAGACGCTGC AGCAGTTCTC GCGCGATGCC
    GATGAGATTG AGAACTGGAT TGCAGAGAAG TTGCAGCTGG CCACCGAAGA GAGCTACAAG
    GACCCGGCTA ACATTCAGTC GAAGCACCAG AAGCACCAGG CCTTCGAGGC GGAGCTGGCG
    GCTAACGCCG ATCGCATCCA GAGCGTGCTA GCCATGGGCG GAAACCTCAT CGATAAGAAG
    CAGTGCAGCG GGTCGGAGGA CGCGGTGCAG AAGCGGCTGA CGCAGATCGC CGATCAGTGG
    GAGTACCTGA CCCATAAGAC TACCGAGAAG TCGCTGAAGC TAAAGGAGGC GAATAAGCAG
    CGCACCTATA TCGCTGCTGT CAAGGATTTG GACTTCTGGT TGGGCGAGGT CGAGAGTCTT
    TTGACCACTG AGGATTCTGG TAAGGATTTG GCCTCTGTCC AAAACCTCAT GAAGAAGCAT
    CAGTTGGTCG AGGCGGATAT CGTGGCGCAC GAAGACCGCA TCAAGGACAT GAACAACCAG
    GCCGATTCCT TGGTGGAGAG CGGCCAGTTC GATACTGCCG GCATCCAGGA GAAGCGTCAA
    AGCATCAATG AGCGCTACGA GCGCATCTGC AACCTGGCGG CCCATCGCCA AGCCCGTCTC
    AACGAGGCTC TGACCCTGCA CCAATTCTTC CGCGACATCG CCGACGAGGA GAGCTGGATC
    AAGGAGAAGA AGCTACTTGT CGGCTCCGAC GACTATGGTC GCGATCTGAC CGGCGTCCAG
    AACCTCAAGA AGAAGCACAA GCGCCTAGAA GCCGAATTGG GCTCTCACGA GCCGGCCATT
    CAAGCTGTGC AGGAGGCTGG CGAGAAGCTC ATGGACGTGT CCAACCTCGG TGTCCCCGAA
    ATTGAGCAGC GCCTGAAGGC TCTGAACCAG GCCTGGGCCG AGCTAAAGAA CCTGGCTGCC
    ACGCGAGGTC AGAAGTTGGA TGAGTCGCTG ACCTATCAGC AATTCCTCGC TCAGGTGGAG
    GAGGAGGAGG CCTGGATCAC CGAGAAGCAA CAGCTGCTCT CTGTGGAGGA CTACGGCGAC
    TCGATGGCCG CCGTACAAGG ACTGCTGAAA AAGCACGACG CATTCGAAAC GGACTTCACT
    GCCCACAAGG ACCGCTGTTC TTTGATCTGC GACCAGGGCA GTGAGTTGGT GGAGGCCAAG
    AACCACCACG GAGAGTCCAT TGCCCAACGC TGCCAGCAGC TGCGCCTGAA ACTGGACAAC
    CTGTCCGCCC TGGCTGCCCG CCGCAAGGGT GCCCTGCTGG ACAACTCGGC CTATCTGCAG
    TTCATGTGGA AGGCCGATGT GGTCGAGAGC TGGATCGACG ACAAGGAGAA CTATGTGCGC
    TCCGATGAGT TCGGACGCGA CCTGTCCACC GTGCAAACGC TGTTGACCAA GCAAGAGACA
    TTCGATGCAG GTCTCAATGC CTTCGAGCAG GAGGGCATAC ACAACATCAC GGCACTTAAG
    GACCAACTGA TAAATGCCAG TCACGCCCAG TCGCCGGCAA TCCTAAAGCG TCACGGAGAC
    GTGATTGCCA GGTGGCAGAA GTTGCGCGAC GCCTCCAACA CGCGCAAGGA CCGACTGCTG
    GCCATGCAGG AGCAGTTCCG CCAGATCGAG GAACTCTACT TGACATTTGC CAAGAAAGCT
    TCGGCCTTCA ATTCTTGGTT CGAAAATGCC GAGGAGGATC TCACAGATCC TGTTCGCTGC
    AATTCGATCG AAGAAATCCG CGCCCTGCGC GACGCCCATG CCCAATTCCA GGCGTCCCTT
    TCGTCGGCCG AAGCTGACTT CAAGGCATTG GCAGCACTCG ACCAGAAGAT CAAGAGCTTT
    AATGTTGGAC CCAACCCGTA CACGTGGTTC ACCATGGAAG CTCTTGAGGA GACCTGGCGT
    AATCTGCAAA AGATCATAGA GGAACGCGAT GGGGAACTTG CCAAGGAGGC CAAGCGGCAG
    GAGGAGAACG ACAAGCTGCG CAAGGAGTTT GCCAAGCACG CCAATCTTTT CCACCAGTGG
    CTAACAGAGA CAAGATATTA TATGCTGGGT TATAACCGTC AAGGAACGTC CATGATGGAA
    GGCTCCGGTT CCCTGGAACA GCAACTGGAG GCGCTTCGTG TCAAGGCCAC CGAGGTGCGC
    GCACGTCGCG TTGACCTCAA GAAGATCGAG GAGCTGGGAG CCTTGCTGGA GGAGCACCTT
    ATTCTGGACA ACCGCTACAC GGAACACTCG ACGGTGGGCC TGGCCCAGCA GTGGGATCAG
    CTTGACCAGC TGTCTATGCG CATGCAGCAC AACTTAGAGC AACAGATCCA GGCACGCAAC
    CACTCGGGCG TCTCCGAGGA CTCACTCAAG GAGTTCTCGA TGATGTTCAA GCACTTCGAT
    AAGGACAAGA GCGGCAAGCT CAACCACCAG GAATTCAAGT CCTGCCTGCG CGCCCTCGGC
    TACGACCTGC CCATGGTGGA GGAGGGACAG CCCGACCCGG AGTTCGAAGC AATTCTCGAT
    GTTGTCGACC CCAACCGCGA TGGCTACGTC TCTCTGCAGG AATACATCGC GTTCATGATC
    TCCAAGGAGA CGGAGAACGT TCAATCCTAC GAGGAGATCG AGAATGCCTT CCGCGCCATC
    ACCGCTGCCG ACCGCCCCTA TGTAACTAAG GAGGAGCTCT ACTGCAACCT CACCAAGGAC
    ATGGCTGACT ACTGCGTGCA GCGCATGAAA CCCTTCTCGG AACCGCGCTC CGGCCAGCCC
    ATCAAGGATG CCCTGGACTA CATAGACTTC ACGCGAACGC TGTTCCAGAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001261286.1
    CDS277..7650
    Translation

    Target ORF information:

    RefSeq Version NM_001274357.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster alpha spectrin, transcript variant B (alpha-Spec), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001274357.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGAGAACT TTACACCCAA AGAGGTGAAG ATCCTCGAGA CAGTCGAGGA CATCCAGGAG 
    CGACGTGAGC AGGTTCTGTC CCGCTACAAT GACTTCAAGA TCGAGACGCG CCAGAAGCGT
    GAAAAGCTCG AGGACTCGCG CCGCTTCCAG TACTTCAAGC GCGATGCCGA CGAGTTGGAG
    TCGTGGATCC ACGAGAAGCT GCAGGCAGCC AGCGAGGAGA GCTACCGCGA TCCGACCAAC
    CTGCAGGCCA AAATCCAGAA ACATCAGGCG TTCGAGGCGG AGGTGTCGGC GCACAGCAAC
    GCCATTGTCT CGCTGGACAA CACTGGCCAG GAGATGATCA ACCAGCAACA TTTTGCCTCC
    GAGTCGATTC AGGTCCGCCT CGACGAGCTG CACAAGCTGT GGGAGCTGCT TCTTAGCCGT
    CTGGCCGAGA AGGGTCTAAA GCTGCAGCAG GCTCTCGTCC TGGTGCAGTT CCTGCGCCAG
    TGCGAGGAGG TGATGTTCTG GATTAAGGAC AAGGAGACCT TTGTCACCGC CGACGAGTTC
    GGCCAAGACC TGGAACATGT AGAGGTTCTG CAGCGCAAGT TCGATGAGTT CCAGAAGGAC
    ATGGCCTCCC AGGAGTATCG CGTGACTGAG GTCAACCAGT TGGCCGATAA GTTGGTGCAA
    GATGGTCATC CCGAGCGCGA CACTATTACC AAGCGCAAGG AGGAGCTAAA CGAGGCTTGG
    CAGCGACTTA AGCAGTTAGC CATTGTGCGC CAAGAGAAGC TATTCGGTGC CCACGAGATC
    CAGCGTTTCA ATCGCGATGC CGACGAAACC GTCGCCTGGA TTGCCGAGAA GGATGTGGTC
    CTGTCGTCCG ATGATTATGG CCGCGATTTG GCCAGTGTTC AGGCGCTGCA ACGCAAACAC
    GAAGGAGTGG AGCGGGATTT GGCCGCCTTG GAGGATAAGG TTTCCACACT GGGAGCCGAG
    GCCCAGCGGC TGTGCTCCAT TCACGCCGAT CACAGTGACC AGATCCGTGA CAAACAGGCC
    GAGATTGCCA ACTACTGGCA GAGTCTCACC ACCAAGGCTC GCGAGCGCAA GCAGAAGCTA
    GATGAGTCCT ACTACCTGCA CCGTTTCCTC GCCGATTTCC GCGACCTCGT GTCCTGGATC
    AATGGCATGA AGGCCATCAT CTCGGCCGAT GAGCTGGCTA AGGATGTGGC TGGAGCAGAG
    GCTCTTCTGG AACGTCACCA GGAGCACAAG GGCGAGATCG ACGCTCGTGA GGATAGCTTC
    AAGCTGACCA CTGAATCCGG ACAGAAGCTG CTGGAGCGTG AGCATTACGC CGCCGCCGAA
    ATCCAGGAAA AGCTTGCCGC TCTGGAAAAC GACAAGAGTT CACTGCTCTC GCTGTGGGAA
    GATCGTCGCA TCCTGTACGA GCAGTGCATG GATCTGCAAC TGTTTTACCG CGATACGGAG
    CAGGCTGACA CTTGGATGGC CAAACAGGAG GCCTTCCTGG CCAACGAGGA CCTTGGCGAC
    TCCCTGGACT CTGTGGAGGC GCTAATTAAG AAGCACGAGG ACTTCGAGAA GAGTCTGGCT
    GCCCAGGAGG AGAAGATCAA GGCTTTAGAC ATCTTTGCCA CCAAATTAAT CGATGGTCAG
    CATTATGCCG CCGATGATGT GGCCCAGCGC CGTCAGATGC TCCTAGCCCG GCGGGCTGCT
    CTCCAAGAAA AATCGTCCAA GCGTCGCCAA TTGCTGGAGG ATTCGAACCG CTACCAGCAG
    TTCGAGCGCG ATTGCGACGA GACCAAGGGC TGGATTAGCG AGAAGCTGAA GTTTGCCACC
    GATGACAGCT ATTTGGATCC TACCAACCTT AACGGAAAGA TGCAGAAGCA CCAGAACTTT
    GAGCACGAGC TGAATGCCAA CAAGTCGCGC ATCGAGGACA TCACCAACGT GGGCACCGAG
    CTGATCGAGA AGCAGCACTA TGCTGCCGAC CAGATCAACA CCCGCATGCA GGAGATCGTC
    GTCCTGTGGG AGACCCTCGT CCAGGCTTCC GATAAAAAGG GAACCAAACT CAACGAGGCA
    TGCCAACAGC AGCAGTTCAA CCGCACCATC GAGGACATTG AGCTGTGGCT TAGCGAGATC
    GAGGGTCAAT TGTTGTCGGA GGATCACGGC AAAGACCTGA CCTCCGTTCA GAATTTGCAG
    AAGAAGCACG CCCTGCTGGA GGCCGATGTG ATGGCTCACC AGGATCGCAT TGAAAGCATC
    AAAGTGGCCG CCAACAAGTT CATCGAATCC GGTCACTTTG ACGCGGACAA CATTCGCAAC
    AAGGAGGGCA ATCTGTCGGC TCGCTATGCC GCCCTAGCTG CACCCATGGG CGAGAGGAAA
    CAACACCTTC TTGACTCCCT TCAGGTGCAG CAGCTCTTCC GCGACTTGGA GGACGAGGCT
    GCCTGGATCC GTGAGAAGGA ACCCATTGCC GCATCCACGA ACCGCGGTCG TGACTTGATT
    GGTGTGCAAA ACCTGATCAA GAAACACCAG GCTGTGCTGG CCGAGATCAA CAACCATGAG
    GCCAGGCTGC TGAATGTGAT CAGTTCGGGC GAGAACATGC TGAAGGACCA GCCCTTCGCC
    AGCGACGATA TCCGTCAGCG TTTGGAGGCG CTCCAAGAGC AGTGGAACAC GCTGAAGGAG
    AAGTCCAGCC AGCGAAAGCA GGATCTGGAT GACTCTCTCC AGGCACACCA GTACTTTGCC
    GACGCCAACG AAGCTGAGTC GTGGATGCGC GAAAAGGAAC CGATTGCAAC TGGCAGTGAT
    TATGGTAAGG ACGAGGATTC CTCGGAGGCT TTGCTCAAGA AGCACGAGGC TCTGGTCTCC
    GATCTAGAGG CTTTTGGCAA CACCATTCAG GCGCTGCAGG AGCAGGCCAA GAACTGTCGC
    CAGCAGGAGA CCCCTGTTGT GGATATCACT GGCAAGGAGT GCGTGGTTGC CCTCTACGAC
    TACACGGAGA AATCCCCTCG TGAGGTCTCG ATGAAGAAGG GCGATGTTCT GACCCTGCTG
    AACTCGAACA ACAAAGACTG GTGGAAGGTG GAGGTCAACG ACCGCCAGGG CTTCGTACCG
    GCCGCATACA TCAAGAAGAT TGACGCCGGC TTGAGCGCCT CCCAGCAGAA CCTAGTGGAC
    AATCATTCGA TTGCCAAGCG CCAGAACCAG ATCAACTCGC AGTACGACAA CTTGCTGGCC
    CTCGCCCGCG AGCGCCAGAA CAAGCTAAAC GAGACTGTGA AGGCGTACGT TCTTGTTCGT
    GAGGCTGCCG ACTTGGCCCA GTGGATCCGG GACAAGGAGA ACCACGCTCA GATCGCTGAT
    GTTGTGGGAG AGGATCTGGA AGAGGTGGAG GTGCTCCAAA AGAAGTTCGA CGACTTCAAC
    GACGACCTGA AGGCCAACGA AGTGCGTCTG GCAAACATGA ACGAGATTGC CGTGCAGCTG
    ACTTCCTTGG GTCAGACCGA GGCCGCCCTA AAGATCCAAA CCCAGATGCA AGACCTGAAC
    GAGAAGTGGA ACAACCTGCA GACGCTGACC GCTGAGAAGG CCAGCCAACT GGGATCCGCC
    CATGAGGTGC AACGCTTCCA TCGTGACATT GACGAGACCA AGGATTGGAT CGCTGAGAAG
    GCCAATGCCC TGAACAACGA CGACCTGGGC AAGGATCTGC GCAGTGTCCA GACTCTGCAG
    CGCAAGCACG AGGGCGTGGA ACGCGACTTG GCCGCATTGC GGGACAAGAT CCGCCAGCTG
    GACGAAACCG CCAACAGGCT GATGCAGTCG CACCCGGACA CCGCCGAGCA GACCTACGCC
    AAGCAGAAGG AGATCAACGA GATGTGGGAC CAGATCATCA CCAAGTCCAC CGCCCGCAAG
    GAGAAGCTGC TGGACTCGTA CGACCTGCAG CGTTTCCTCA GCGACTACCG CGATCTGCTG
    GCCTGGATCA ACTCGATGAT GAGCCTGGTC ACGTCCGACG AGCTGGCCAA CGATGTAACA
    GGAGCCGAGG CTCTGATCGA GCGTCATCAG GCACGCCGTG CCGAGATTGG GTTCACATTG
    GGCATCAGCT CGGCTCCCGG AGCCGCCGCC TCGACCTCCA GCATCGCATC TCCATCCGGC
    GACGAGGAAC ACCGAACGGA GATCGATGCG CGCGCCGGAA CTTTCGGGGC TTTCGAGCAG
    TTCGGAAACG AGCTGCTGCA GGCCAACCAC TATGCGTCGC CTGAAATCAA GGAGAAAATC
    GAGGATCTGG CCAAGGCCCG CGAGGACTTG GAGAAGGCGT GGACAGAGCG CCGCCTGCAG
    CTGGAGCAGA ACTTGGATCT GCAGCTCTAC ATGCGTGACT GCGAGTTGGC CGAGTCCTGG
    ATGTCAGCCC GCGAGGCCTT CCTAAATGCC GATGATGATG CCAATGCCGG CGGAAATGTG
    GAGGCACTGA TCAAGAAGCA CGAGGACTTC GACAAGGCCA TTAACGGCCA CGAACAGAAG
    ATTGCTGCTC TGCAGACGGT GGCGGATCAG TTGATCGCCC AGAACCACTA TGCCTCCAAT
    TTGGTGGACG AGAAGCGCAA GCAAGTGCTG GAGCGCTGGC GCCATCTCAA GGAGGGTCTG
    ATCGAGAAGC GATCCCGTTT GGGTGACGAG CAGACGCTGC AGCAGTTCTC GCGCGATGCC
    GATGAGATTG AGAACTGGAT TGCAGAGAAG TTGCAGCTGG CCACCGAAGA GAGCTACAAG
    GACCCGGCTA ACATTCAGTC GAAGCACCAG AAGCACCAGG CCTTCGAGGC GGAGCTGGCG
    GCTAACGCCG ATCGCATCCA GAGCGTGCTA GCCATGGGCG GAAACCTCAT CGATAAGAAG
    CAGTGCAGCG GGTCGGAGGA CGCGGTGCAG AAGCGGCTGA CGCAGATCGC CGATCAGTGG
    GAGTACCTGA CCCATAAGAC TACCGAGAAG TCGCTGAAGC TAAAGGAGGC GAATAAGCAG
    CGCACCTATA TCGCTGCTGT CAAGGATTTG GACTTCTGGT TGGGCGAGGT CGAGAGTCTT
    TTGACCACTG AGGATTCTGG TAAGGATTTG GCCTCTGTCC AAAACCTCAT GAAGAAGCAT
    CAGTTGGTCG AGGCGGATAT CGTGGCGCAC GAAGACCGCA TCAAGGACAT GAACAACCAG
    GCCGATTCCT TGGTGGAGAG CGGCCAGTTC GATACTGCCG GCATCCAGGA GAAGCGTCAA
    AGCATCAATG AGCGCTACGA GCGCATCTGC AACCTGGCGG CCCATCGCCA AGCCCGTCTC
    AACGAGGCTC TGACCCTGCA CCAATTCTTC CGCGACATCG CCGACGAGGA GAGCTGGATC
    AAGGAGAAGA AGCTACTTGT CGGCTCCGAC GACTATGGTC GCGATCTGAC CGGCGTCCAG
    AACCTCAAGA AGAAGCACAA GCGCCTAGAA GCCGAATTGG GCTCTCACGA GCCGGCCATT
    CAAGCTGTGC AGGAGGCTGG CGAGAAGCTC ATGGACGTGT CCAACCTCGG TGTCCCCGAA
    ATTGAGCAGC GCCTGAAGGC TCTGAACCAG GCCTGGGCCG AGCTAAAGAA CCTGGCTGCC
    ACGCGAGGTC AGAAGTTGGA TGAGTCGCTG ACCTATCAGC AATTCCTCGC TCAGGTGGAG
    GAGGAGGAGG CCTGGATCAC CGAGAAGCAA CAGCTGCTCT CTGTGGAGGA CTACGGCGAC
    TCGATGGCCG CCGTACAAGG ACTGCTGAAA AAGCACGACG CATTCGAAAC GGACTTCACT
    GCCCACAAGG ACCGCTGTTC TTTGATCTGC GACCAGGGCA GTGAGTTGGT GGAGGCCAAG
    AACCACCACG GAGAGTCCAT TGCCCAACGC TGCCAGCAGC TGCGCCTGAA ACTGGACAAC
    CTGTCCGCCC TGGCTGCCCG CCGCAAGGGT GCCCTGCTGG ACAACTCGGC CTATCTGCAG
    TTCATGTGGA AGGCCGATGT GGTCGAGAGC TGGATCGACG ACAAGGAGAA CTATGTGCGC
    TCCGATGAGT TCGGACGCGA CCTGTCCACC GTGCAAACGC TGTTGACCAA GCAAGAGACA
    TTCGATGCAG GTCTCAATGC CTTCGAGCAG GAGGGCATAC ACAACATCAC GGCACTTAAG
    GACCAACTGA TAAATGCCAG TCACGCCCAG TCGCCGGCAA TCCTAAAGCG TCACGGAGAC
    GTGATTGCCA GGTGGCAGAA GTTGCGCGAC GCCTCCAACA CGCGCAAGGA CCGACTGCTG
    GCCATGCAGG AGCAGTTCCG CCAGATCGAG GAACTCTACT TGACATTTGC CAAGAAAGCT
    TCGGCCTTCA ATTCTTGGTT CGAAAATGCC GAGGAGGATC TCACAGATCC TGTTCGCTGC
    AATTCGATCG AAGAAATCCG CGCCCTGCGC GACGCCCATG CCCAATTCCA GGCGTCCCTT
    TCGTCGGCCG AAGCTGACTT CAAGGCATTG GCAGCACTCG ACCAGAAGAT CAAGAGCTTT
    AATGTTGGAC CCAACCCGTA CACGTGGTTC ACCATGGAAG CTCTTGAGGA GACCTGGCGT
    AATCTGCAAA AGATCATAGA GGAACGCGAT GGGGAACTTG CCAAGGAGGC CAAGCGGCAG
    GAGGAGAACG ACAAGCTGCG CAAGGAGTTT GCCAAGCACG CCAATCTTTT CCACCAGTGG
    CTAACAGAGA CAAGATATTA TATGCTGGGT TATAACCGTC AAGGAACGTC CATGATGGAA
    GGCTCCGGTT CCCTGGAACA GCAACTGGAG GCGCTTCGTG TCAAGGCCAC CGAGGTGCGC
    GCACGTCGCG TTGACCTCAA GAAGATCGAG GAGCTGGGAG CCTTGCTGGA GGAGCACCTT
    ATTCTGGACA ACCGCTACAC GGAACACTCG ACGGTGGGCC TGGCCCAGCA GTGGGATCAG
    CTTGACCAGC TGTCTATGCG CATGCAGCAC AACTTAGAGC AACAGATCCA GGCACGCAAC
    CACTCGGGCG TCTCCGAGGA CTCACTCAAG GAGTTCTCGA TGATGTTCAA GCACTTCGAT
    AAGGACAAGA GCGGCAAGCT CAACCACCAG GAATTCAAGT CCTGCCTGCG CGCCCTCGGC
    TACGACCTGC CCATGGTGGA GGAGGGACAG CCCGACCCGG AGTTCGAAGC AATTCTCGAT
    GTTGTCGACC CCAACCGCGA TGGCTACGTC TCTCTGCAGG AATACATCGC GTTCATGATC
    TCCAAGGAGA CGGAGAACGT TCAATCCTAC GAGGAGATCG AGAATGCCTT CCGCGCCATC
    ACCGCTGCCG ACCGCCCCTA TGTAACTAAG GAGGAGCTCT ACTGCAACCT CACCAAGGAC
    ATGGCTGACT ACTGCGTGCA GCGCATGAAA CCCTTCTCGG AACCGCGCTC CGGCCAGCCC
    ATCAAGGATG CCCTGGACTA CATAGACTTC ACGCGAACGC TGTTCCAGAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.