nSyb cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	nSyb
Entrez Gene ID	38196
Full Name	neuronal Synaptobrevin
Synonyms	CG17248,Dmel\CG17248,Dn-syb,N-SYB,N-Syb,N-syb,NSYB,Nsyb,SYB,Syb,Syb62A,n-Syb,n-syb,nSYB,nsyb,syb
General protein information	Preferred Names neuronal Synaptobrevin Names CG17248 gene product from transcript CG17248-RI CG17248-PA CG17248-PE CG17248-PF CG17248-PG CG17248-PH CG17248-PI CG17248-PJ CG17248-PK N-synaptobrevin Neuronal-synaptobrevin Synaptobrevin-62A VAMP3 nSyb-PA nSyb-PE nSyb-PF nSyb-PG nSyb-PH nSyb-PI nSyb-PJ nSyb-PK neural-synaptobrevin neuronal SYNAPTOBREVIN neuronal synaptobrevin neuronal synaptogrevin synaptobrevin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 62A9-62A9

mRNA and Protein(s)

mRNA	Protein	Name
NM_167904.3	NP_728644.1	neuronal synaptobrevin, isoform F
NM_167906.3	NP_728646.2	neuronal synaptobrevin, isoform H
NM_057710.6	NP_477058.1	neuronal synaptobrevin, isoform A
NM_001274340.1	NP_001261269.1	neuronal synaptobrevin, isoform J
NM_206234.4	NP_995956.1	neuronal synaptobrevin, isoform E
NM_167905.4	NP_728645.1	neuronal synaptobrevin, isoform G
NM_001274341.1	NP_001261270.1	neuronal synaptobrevin, isoform K
NM_001259618.2	NP_001246547.1	neuronal synaptobrevin, isoform I

Pathways from BioSystems

Homologies

Bos taurus (cattle)	VAMP2	NP_776908.1
Drosophila melanogaster (fruit fly)	n-syb	NP_001261270.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005507	XP_001688670.1
Pan troglodytes (chimpanzee)	VAMP2	XP_001156324.1
Mus musculus (house mouse)	Vamp2	NP_033523.1
Danio rerio (zebrafish)	vamp2	NP_956299.1
	VAMP2	NP_001027992.1
Canis lupus familiaris (dog)	VAMP2	XP_005620068.1
Rattus norvegicus (Norway rat)	Vamp2	NP_036795.1
Gallus gallus (chicken)	VAMP2	XP_422640.2
Xenopus tropicalis (tropical clawed frog)	vamp2	NP_989031.1
Homo sapiens (human)	VAMP2	NP_055047.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

data indicate that juxtamembrane tryptophan residues are important determinants of the position of Synaptobrevin in the membrane
Title: Effect of juxtamembrane tryptophans on the immersion depth of Synaptobrevin, an integral vesicle membrane protein.

a role for n-Syb in a neuron-specific sort-and-degrade mechanism that protects neurons from degeneration.
Title: The synaptic vesicle SNARE neuronal Synaptobrevin promotes endolysosomal degradation and prevents neurodegeneration.

Our study suggests a novel, cell type-specific function of n-Syb in synaptogenesis and it distinguishes between two synapse types: TNT resistant and TNT sensitive ones.
Title: Distinct functions of neuronal synaptobrevin in developing and mature fly photoreceptors.

Expression of syb can restore synaptic transmission to n-syb mutants as assayed both by electroretinogram and recordings of excitatory junctional currents at the neuromuscular junction.
Title: Members of the synaptobrevin/vesicle-associated membrane protein (VAMP) family in Drosophila are functionally interchangeable in vivo for neurotransmitter release and cell viability.

The following nSyb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nSyb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa17188	NM_206234.4 Latest version!	Drosophila melanogaster neuronal synaptobrevin, transcript variant E (nSyb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa17188	NM_167905.4 Latest version!	Drosophila melanogaster neuronal synaptobrevin, transcript variant G (nSyb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa17189	NM_057710.6 Latest version!	Drosophila melanogaster neuronal synaptobrevin, transcript variant A (nSyb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa17189	NM_167904.3 Latest version!	Drosophila melanogaster neuronal synaptobrevin, transcript variant F (nSyb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa17190	NM_167906.3 Latest version!	Drosophila melanogaster neuronal synaptobrevin, transcript variant H (nSyb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa17190	NM_001259618.2 Latest version!	Drosophila melanogaster neuronal synaptobrevin, transcript variant I (nSyb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa20426	NM_001274340.1 Latest version!	Drosophila melanogaster neuronal synaptobrevin, transcript variant J (nSyb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa20427	NM_001274341.1 Latest version!	Drosophila melanogaster neuronal synaptobrevin, transcript variant K (nSyb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nSyb ( NM_206234.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995956.1 Drosophila melanogaster neuronal synaptobrevin, transcript variant E (nSyb), mRNA.

Price & Availability↑

CloneID	OFa17188
Clone ID Related Accession (Same CDS sequence)	NM_206234.4 , NM_167905.4
Accession Version	NM_206234.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neuronal synaptobrevin, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_206234.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGAAAGA AAGACAAGAA CAAGGAACAG GCGGACGCTG CACCAGCTGG CGATGCACCA 
CCCAATGCCG GAGCCCCGGC CGGAGAGGGC GGCGATGGCG AGATTGTGGG CGGACCACAC 
AATCCGCAGC AGATCGCGGC ACAGAAGCGT CTGCAGCAGA CGCAGGCGCA GGTCGATGAG 
GTCGTGGACA TCATGCGCAC GAACGTGGAG AAGGTGCTGG AGCGCGACAG CAAGCTGTCG 
GAGCTGGACG ACCGTGCCGA TGCCTTGCAG CAGGGTGCCT CGCAGTTTGA GCAGCAGGCG 
GGCAAGCTCA AGAGGAAATT CTGGCTTCAG AACTTAAAGA TGATGATCAT CATGGGCGTG 
ATTGGCCTGG TTGTCGTGGG CATTATTGCA AATAAACTTG GACTCATAGG CGGGGAGCAG 
CCGCCACAGT ACCAGTATCC CCCACAGTAC ATGCAGCCAC CGCCGCCGCC ACCGCAGCAG 
CCAGCCGGAG GACAGTCATC GCTGGTGGAT GCGGCAGGAG CAGGAGACGG AGCAGGAGCA 
GGAGGATCGG CAGGAGCTGG CGATCACGGC GGCGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995956.1
CDS	527..1105
Translation	MGKKDKNKEQADAAPAGDAPPNAGAPAGEGGDGEIVGGPHNPQQIAAQKRLQQTQAQVDEVVDIMRTNVEKVLERDSKLSELDDRADALQQGASQFEQQAGKLKRKFWLQNLKMMIIMGVIGLVVVGIIANKLGLIGGEQPPQYQYPPQYMQPPPPPPQQPAGGQSSLVDAAGAGDGAGAGGSAGAGDHGGV

Target ORF information:

RefSeq Version	NM_206234.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neuronal synaptobrevin, transcript variant E (nSyb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206234.4

ORF Insert Sequence:

ATGGGAAAGA AAGACAAGAA CAAGGAACAG GCGGACGCTG CACCAGCTGG CGATGCACCA 
CCCAATGCCG GAGCCCCGGC CGGAGAGGGC GGCGATGGCG AGATTGTGGG CGGACCACAC 
AATCCGCAGC AGATCGCGGC ACAGAAGCGT CTGCAGCAGA CGCAGGCGCA GGTCGATGAG 
GTCGTGGACA TCATGCGCAC GAACGTGGAG AAGGTGCTGG AGCGCGACAG CAAGCTGTCG 
GAGCTGGACG ACCGTGCCGA TGCCTTGCAG CAGGGTGCCT CGCAGTTTGA GCAGCAGGCG 
GGCAAGCTCA AGAGGAAATT CTGGCTTCAG AACTTAAAGA TGATGATCAT CATGGGCGTG 
ATTGGCCTGG TTGTCGTGGG CATTATTGCA AATAAACTTG GACTCATAGG CGGGGAGCAG 
CCGCCACAGT ACCAGTATCC CCCACAGTAC ATGCAGCCAC CGCCGCCGCC ACCGCAGCAG 
CCAGCCGGAG GACAGTCATC GCTGGTGGAT GCGGCAGGAG CAGGAGACGG AGCAGGAGCA 
GGAGGATCGG CAGGAGCTGG CGATCACGGC GGCGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nSyb ( NM_167905.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728645.1 Drosophila melanogaster neuronal synaptobrevin, transcript variant G (nSyb), mRNA.

Price & Availability↑

CloneID	OFa17188
Clone ID Related Accession (Same CDS sequence)	NM_206234.4 , NM_167905.4
Accession Version	NM_167905.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neuronal synaptobrevin, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_167905.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGAAAGA AAGACAAGAA CAAGGAACAG GCGGACGCTG CACCAGCTGG CGATGCACCA 
CCCAATGCCG GAGCCCCGGC CGGAGAGGGC GGCGATGGCG AGATTGTGGG CGGACCACAC 
AATCCGCAGC AGATCGCGGC ACAGAAGCGT CTGCAGCAGA CGCAGGCGCA GGTCGATGAG 
GTCGTGGACA TCATGCGCAC GAACGTGGAG AAGGTGCTGG AGCGCGACAG CAAGCTGTCG 
GAGCTGGACG ACCGTGCCGA TGCCTTGCAG CAGGGTGCCT CGCAGTTTGA GCAGCAGGCG 
GGCAAGCTCA AGAGGAAATT CTGGCTTCAG AACTTAAAGA TGATGATCAT CATGGGCGTG 
ATTGGCCTGG TTGTCGTGGG CATTATTGCA AATAAACTTG GACTCATAGG CGGGGAGCAG 
CCGCCACAGT ACCAGTATCC CCCACAGTAC ATGCAGCCAC CGCCGCCGCC ACCGCAGCAG 
CCAGCCGGAG GACAGTCATC GCTGGTGGAT GCGGCAGGAG CAGGAGACGG AGCAGGAGCA 
GGAGGATCGG CAGGAGCTGG CGATCACGGC GGCGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728645.1
CDS	530..1108
Translation	MGKKDKNKEQADAAPAGDAPPNAGAPAGEGGDGEIVGGPHNPQQIAAQKRLQQTQAQVDEVVDIMRTNVEKVLERDSKLSELDDRADALQQGASQFEQQAGKLKRKFWLQNLKMMIIMGVIGLVVVGIIANKLGLIGGEQPPQYQYPPQYMQPPPPPPQQPAGGQSSLVDAAGAGDGAGAGGSAGAGDHGGV

Target ORF information:

RefSeq Version	NM_167905.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neuronal synaptobrevin, transcript variant G (nSyb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167905.4

ORF Insert Sequence:

ATGGGAAAGA AAGACAAGAA CAAGGAACAG GCGGACGCTG CACCAGCTGG CGATGCACCA 
CCCAATGCCG GAGCCCCGGC CGGAGAGGGC GGCGATGGCG AGATTGTGGG CGGACCACAC 
AATCCGCAGC AGATCGCGGC ACAGAAGCGT CTGCAGCAGA CGCAGGCGCA GGTCGATGAG 
GTCGTGGACA TCATGCGCAC GAACGTGGAG AAGGTGCTGG AGCGCGACAG CAAGCTGTCG 
GAGCTGGACG ACCGTGCCGA TGCCTTGCAG CAGGGTGCCT CGCAGTTTGA GCAGCAGGCG 
GGCAAGCTCA AGAGGAAATT CTGGCTTCAG AACTTAAAGA TGATGATCAT CATGGGCGTG 
ATTGGCCTGG TTGTCGTGGG CATTATTGCA AATAAACTTG GACTCATAGG CGGGGAGCAG 
CCGCCACAGT ACCAGTATCC CCCACAGTAC ATGCAGCCAC CGCCGCCGCC ACCGCAGCAG 
CCAGCCGGAG GACAGTCATC GCTGGTGGAT GCGGCAGGAG CAGGAGACGG AGCAGGAGCA 
GGAGGATCGG CAGGAGCTGG CGATCACGGC GGCGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nSyb ( NM_167904.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728644.1 Drosophila melanogaster neuronal synaptobrevin, transcript variant F (nSyb), mRNA.

Price & Availability↑

CloneID	OFa17189
Clone ID Related Accession (Same CDS sequence)	NM_167904.3 , NM_057710.6
Accession Version	NM_167904.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neuronal synaptobrevin, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_167904.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGACG CTGCACCAGC TGGCGATGCA CCACCCAATG CCGGAGCCCC GGCCGGAGAG 
GGCGGCGATG GCGAGATTGT GGGCGGACCA CACAATCCGC AGCAGATCGC GGCACAGAAG 
CGTCTGCAGC AGACGCAGGC GCAGGTCGAT GAGGTCGTGG ACATCATGCG CACGAACGTG 
GAGAAGGTGC TGGAGCGCGA CAGCAAGCTG TCGGAGCTGG ACGACCGTGC CGATGCCTTG 
CAGCAGGGTG CCTCGCAGTT TGAGCAGCAG GCGGGCAAGC TCAAGAGGAA ATTCTGGCTT 
CAGAACTTAA AGATGATGAT CATCATGGGC GTGATTGGCC TGGTTGTCGT GGGCATTATT 
GCAAATAAAC TTGGACTCAT AGGCGGGGAG CAGCCGCCAC AGTACCAGTA TCCCCCACAG 
TACATGCAGC CACCGCCGCC GCCACCGCAG CAGCCAGCCG GAGGACAGTC ATCGCTGGTG 
GATGCGGCAG GAGCAGGAGA CGGAGCAGGA GCAGGAGGAT CGGCAGGAGC TGGCGATCAC 
GGCGGCGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728644.1
CDS	484..1035
Translation	MADAAPAGDAPPNAGAPAGEGGDGEIVGGPHNPQQIAAQKRLQQTQAQVDEVVDIMRTNVEKVLERDSKLSELDDRADALQQGASQFEQQAGKLKRKFWLQNLKMMIIMGVIGLVVVGIIANKLGLIGGEQPPQYQYPPQYMQPPPPPPQQPAGGQSSLVDAAGAGDGAGAGGSAGAGDHGGV

Target ORF information:

RefSeq Version	NM_167904.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neuronal synaptobrevin, transcript variant F (nSyb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167904.3

ORF Insert Sequence:

ATGGCGGACG CTGCACCAGC TGGCGATGCA CCACCCAATG CCGGAGCCCC GGCCGGAGAG 
GGCGGCGATG GCGAGATTGT GGGCGGACCA CACAATCCGC AGCAGATCGC GGCACAGAAG 
CGTCTGCAGC AGACGCAGGC GCAGGTCGAT GAGGTCGTGG ACATCATGCG CACGAACGTG 
GAGAAGGTGC TGGAGCGCGA CAGCAAGCTG TCGGAGCTGG ACGACCGTGC CGATGCCTTG 
CAGCAGGGTG CCTCGCAGTT TGAGCAGCAG GCGGGCAAGC TCAAGAGGAA ATTCTGGCTT 
CAGAACTTAA AGATGATGAT CATCATGGGC GTGATTGGCC TGGTTGTCGT GGGCATTATT 
GCAAATAAAC TTGGACTCAT AGGCGGGGAG CAGCCGCCAC AGTACCAGTA TCCCCCACAG 
TACATGCAGC CACCGCCGCC GCCACCGCAG CAGCCAGCCG GAGGACAGTC ATCGCTGGTG 
GATGCGGCAG GAGCAGGAGA CGGAGCAGGA GCAGGAGGAT CGGCAGGAGC TGGCGATCAC 
GGCGGCGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nSyb ( NM_057710.6 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477058.1 Drosophila melanogaster neuronal synaptobrevin, transcript variant A (nSyb), mRNA.

Price & Availability↑

CloneID	OFa17189
Clone ID Related Accession (Same CDS sequence)	NM_167904.3 , NM_057710.6
Accession Version	NM_057710.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neuronal synaptobrevin, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_057710.5. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGACG CTGCACCAGC TGGCGATGCA CCACCCAATG CCGGAGCCCC GGCCGGAGAG 
GGCGGCGATG GCGAGATTGT GGGCGGACCA CACAATCCGC AGCAGATCGC GGCACAGAAG 
CGTCTGCAGC AGACGCAGGC GCAGGTCGAT GAGGTCGTGG ACATCATGCG CACGAACGTG 
GAGAAGGTGC TGGAGCGCGA CAGCAAGCTG TCGGAGCTGG ACGACCGTGC CGATGCCTTG 
CAGCAGGGTG CCTCGCAGTT TGAGCAGCAG GCGGGCAAGC TCAAGAGGAA ATTCTGGCTT 
CAGAACTTAA AGATGATGAT CATCATGGGC GTGATTGGCC TGGTTGTCGT GGGCATTATT 
GCAAATAAAC TTGGACTCAT AGGCGGGGAG CAGCCGCCAC AGTACCAGTA TCCCCCACAG 
TACATGCAGC CACCGCCGCC GCCACCGCAG CAGCCAGCCG GAGGACAGTC ATCGCTGGTG 
GATGCGGCAG GAGCAGGAGA CGGAGCAGGA GCAGGAGGAT CGGCAGGAGC TGGCGATCAC 
GGCGGCGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477058.1
CDS	481..1032
Translation	MADAAPAGDAPPNAGAPAGEGGDGEIVGGPHNPQQIAAQKRLQQTQAQVDEVVDIMRTNVEKVLERDSKLSELDDRADALQQGASQFEQQAGKLKRKFWLQNLKMMIIMGVIGLVVVGIIANKLGLIGGEQPPQYQYPPQYMQPPPPPPQQPAGGQSSLVDAAGAGDGAGAGGSAGAGDHGGV

Target ORF information:

RefSeq Version	NM_057710.6
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neuronal synaptobrevin, transcript variant A (nSyb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057710.6

ORF Insert Sequence:

ATGGCGGACG CTGCACCAGC TGGCGATGCA CCACCCAATG CCGGAGCCCC GGCCGGAGAG 
GGCGGCGATG GCGAGATTGT GGGCGGACCA CACAATCCGC AGCAGATCGC GGCACAGAAG 
CGTCTGCAGC AGACGCAGGC GCAGGTCGAT GAGGTCGTGG ACATCATGCG CACGAACGTG 
GAGAAGGTGC TGGAGCGCGA CAGCAAGCTG TCGGAGCTGG ACGACCGTGC CGATGCCTTG 
CAGCAGGGTG CCTCGCAGTT TGAGCAGCAG GCGGGCAAGC TCAAGAGGAA ATTCTGGCTT 
CAGAACTTAA AGATGATGAT CATCATGGGC GTGATTGGCC TGGTTGTCGT GGGCATTATT 
GCAAATAAAC TTGGACTCAT AGGCGGGGAG CAGCCGCCAC AGTACCAGTA TCCCCCACAG 
TACATGCAGC CACCGCCGCC GCCACCGCAG CAGCCAGCCG GAGGACAGTC ATCGCTGGTG 
GATGCGGCAG GAGCAGGAGA CGGAGCAGGA GCAGGAGGAT CGGCAGGAGC TGGCGATCAC 
GGCGGCGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nSyb ( NM_167906.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728646.2 Drosophila melanogaster neuronal synaptobrevin, transcript variant H (nSyb), mRNA.

Price & Availability↑

CloneID	OFa17190
Clone ID Related Accession (Same CDS sequence)	NM_167906.3 , NM_001259618.2
Accession Version	NM_167906.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neuronal synaptobrevin, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_167906.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGAAAGA AAGACAAGAA CAAGGAACAG GCGGACGCTG CACCAGCTGG CGATGCACCA 
CCCAATGCCG GAGCCCCGGC CGGAGAGGGC GGCGATGGCG AGATTGTGGG CGGACCACAC 
AATCCGCAGC AGATCGCGGC ACAGAAGCGT CTGCAGCAGA CGCAGGCGCA GGTCGATGAG 
GTCGTGGACA TCATGCGCAC GAACGTGGAG AAGGTGCTGG AGCGCGACAG CAAGCTGTCG 
GAGCTGGACG ACCGTGCCGA TGCCTTGCAG CAGGGTGCCT CGCAGTTTGA GCAGCAGGCG 
GGCAAGCTCA AGAGGAAATT CTGGCTTCAG AACTTAAAGA TGATGATCAT CATGGGCGTG 
ATTGGCCTGG TTGTCGTGGG CATTATTGCA AAGAAAGACG AAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728646.2
CDS	527..934
Translation	MGKKDKNKEQADAAPAGDAPPNAGAPAGEGGDGEIVGGPHNPQQIAAQKRLQQTQAQVDEVVDIMRTNVEKVLERDSKLSELDDRADALQQGASQFEQQAGKLKRKFWLQNLKMMIIMGVIGLVVVGIIAKKDEE

Target ORF information:

RefSeq Version	NM_167906.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neuronal synaptobrevin, transcript variant H (nSyb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167906.3

ORF Insert Sequence:

ATGGGAAAGA AAGACAAGAA CAAGGAACAG GCGGACGCTG CACCAGCTGG CGATGCACCA 
CCCAATGCCG GAGCCCCGGC CGGAGAGGGC GGCGATGGCG AGATTGTGGG CGGACCACAC 
AATCCGCAGC AGATCGCGGC ACAGAAGCGT CTGCAGCAGA CGCAGGCGCA GGTCGATGAG 
GTCGTGGACA TCATGCGCAC GAACGTGGAG AAGGTGCTGG AGCGCGACAG CAAGCTGTCG 
GAGCTGGACG ACCGTGCCGA TGCCTTGCAG CAGGGTGCCT CGCAGTTTGA GCAGCAGGCG 
GGCAAGCTCA AGAGGAAATT CTGGCTTCAG AACTTAAAGA TGATGATCAT CATGGGCGTG 
ATTGGCCTGG TTGTCGTGGG CATTATTGCA AAGAAAGACG AAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nSyb ( NM_001259618.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246547.1 Drosophila melanogaster neuronal synaptobrevin, transcript variant I (nSyb), mRNA.

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CloneID	OFa17190
Clone ID Related Accession (Same CDS sequence)	NM_167906.3 , NM_001259618.2
Accession Version	NM_001259618.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neuronal synaptobrevin, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001259618.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGAAAGA AAGACAAGAA CAAGGAACAG GCGGACGCTG CACCAGCTGG CGATGCACCA 
CCCAATGCCG GAGCCCCGGC CGGAGAGGGC GGCGATGGCG AGATTGTGGG CGGACCACAC 
AATCCGCAGC AGATCGCGGC ACAGAAGCGT CTGCAGCAGA CGCAGGCGCA GGTCGATGAG 
GTCGTGGACA TCATGCGCAC GAACGTGGAG AAGGTGCTGG AGCGCGACAG CAAGCTGTCG 
GAGCTGGACG ACCGTGCCGA TGCCTTGCAG CAGGGTGCCT CGCAGTTTGA GCAGCAGGCG 
GGCAAGCTCA AGAGGAAATT CTGGCTTCAG AACTTAAAGA TGATGATCAT CATGGGCGTG 
ATTGGCCTGG TTGTCGTGGG CATTATTGCA AAGAAAGACG AAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246547.1
CDS	527..934
Translation	MGKKDKNKEQADAAPAGDAPPNAGAPAGEGGDGEIVGGPHNPQQIAAQKRLQQTQAQVDEVVDIMRTNVEKVLERDSKLSELDDRADALQQGASQFEQQAGKLKRKFWLQNLKMMIIMGVIGLVVVGIIAKKDEE

Target ORF information:

RefSeq Version	NM_001259618.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neuronal synaptobrevin, transcript variant I (nSyb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259618.2

ORF Insert Sequence:

ATGGGAAAGA AAGACAAGAA CAAGGAACAG GCGGACGCTG CACCAGCTGG CGATGCACCA 
CCCAATGCCG GAGCCCCGGC CGGAGAGGGC GGCGATGGCG AGATTGTGGG CGGACCACAC 
AATCCGCAGC AGATCGCGGC ACAGAAGCGT CTGCAGCAGA CGCAGGCGCA GGTCGATGAG 
GTCGTGGACA TCATGCGCAC GAACGTGGAG AAGGTGCTGG AGCGCGACAG CAAGCTGTCG 
GAGCTGGACG ACCGTGCCGA TGCCTTGCAG CAGGGTGCCT CGCAGTTTGA GCAGCAGGCG 
GGCAAGCTCA AGAGGAAATT CTGGCTTCAG AACTTAAAGA TGATGATCAT CATGGGCGTG 
ATTGGCCTGG TTGTCGTGGG CATTATTGCA AAGAAAGACG AAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nSyb ( NM_001274340.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261269.1 Drosophila melanogaster neuronal synaptobrevin, transcript variant J (nSyb), mRNA.

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CloneID	OFa20426
Clone ID Related Accession (Same CDS sequence)	NM_001274340.1
Accession Version	NM_001274340.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neuronal synaptobrevin, isoform J
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGACG CTGCACCAGC TGGCGATGCA CCACCCAATG CCGGAGCCCC GGCCGGAGAG 
GGCGGCGATG GCGAGATTGT GGGCGGACCA CACAATCCGC AGCAGATCGC GGCACAGAAG 
CGTCTGCAGC AGACGCAGGC GCAGGTCGAT GAGGTCGTGG ACATCATGCG CACGAACGTG 
GAGAAGGTGC TGGAGCGCGA CAGCAAGCTG TCGGAGCTGG ACGACCGTGC CGATGCCTTG 
CAGCAGGGTG CCTCGCAGTT TGAGCAGCAG GCGGGCAAGC TCAAGAGGAA ATTCTGGCTT 
CAGAACTTAA AGATGATGAT CATCATGGGC GTGATTGGCC TGGTTGTCGT GGGCATTATT 
GCAAAACCGT ACATGTCGGA TGATAATGCT GCTCCGCAGC CTCCTGCCTT GCAGCAGCAG 
GTGGCTCCGG CATCGGCAAC TGCACCTGAT TCAGCACCAC CACCACCACC ACAACTACTA 
CAACAACAAG AACAACAACT ACAACCACAC CACCATACAC AAAATCTAAA ATCTGAACCA 
CCGACACTCG AGAAACCATT GGCCGAGAAA TTGACCGACC ACCCACAGAG TCCGTCGAAT 
GAAGTGGCGA CTGGACAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261269.1
CDS	493..1113
Translation	MADAAPAGDAPPNAGAPAGEGGDGEIVGGPHNPQQIAAQKRLQQTQAQVDEVVDIMRTNVEKVLERDSKLSELDDRADALQQGASQFEQQAGKLKRKFWLQNLKMMIIMGVIGLVVVGIIAKPYMSDDNAAPQPPALQQQVAPASATAPDSAPPPPPQLLQQQEQQLQPHHHTQNLKSEPPTLEKPLAEKLTDHPQSPSNEVATGQ

Target ORF information:

RefSeq Version	NM_001274340.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neuronal synaptobrevin, transcript variant J (nSyb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274340.1

ORF Insert Sequence:

ATGGCGGACG CTGCACCAGC TGGCGATGCA CCACCCAATG CCGGAGCCCC GGCCGGAGAG 
GGCGGCGATG GCGAGATTGT GGGCGGACCA CACAATCCGC AGCAGATCGC GGCACAGAAG 
CGTCTGCAGC AGACGCAGGC GCAGGTCGAT GAGGTCGTGG ACATCATGCG CACGAACGTG 
GAGAAGGTGC TGGAGCGCGA CAGCAAGCTG TCGGAGCTGG ACGACCGTGC CGATGCCTTG 
CAGCAGGGTG CCTCGCAGTT TGAGCAGCAG GCGGGCAAGC TCAAGAGGAA ATTCTGGCTT 
CAGAACTTAA AGATGATGAT CATCATGGGC GTGATTGGCC TGGTTGTCGT GGGCATTATT 
GCAAAACCGT ACATGTCGGA TGATAATGCT GCTCCGCAGC CTCCTGCCTT GCAGCAGCAG 
GTGGCTCCGG CATCGGCAAC TGCACCTGAT TCAGCACCAC CACCACCACC ACAACTACTA 
CAACAACAAG AACAACAACT ACAACCACAC CACCATACAC AAAATCTAAA ATCTGAACCA 
CCGACACTCG AGAAACCATT GGCCGAGAAA TTGACCGACC ACCCACAGAG TCCGTCGAAT 
GAAGTGGCGA CTGGACAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nSyb ( NM_001274341.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261270.1 Drosophila melanogaster neuronal synaptobrevin, transcript variant K (nSyb), mRNA.

Price & Availability↑

CloneID	OFa20427
Clone ID Related Accession (Same CDS sequence)	NM_001274341.1
Accession Version	NM_001274341.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neuronal synaptobrevin, isoform K
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGACG CTGCACCAGC TGGCGATGCA CCACCCAATG CCGGAGCCCC GGCCGGAGAG 
GGCGGCGATG GCGAGATTGT GGGCGGACCA CACAATCCGC AGCAGATCGC GGCACAGAAG 
CGTCTGCAGC AGACGCAGGC GCAGGTCGAT GAGGTCGTGG ACATCATGCG CACGAACGTG 
GAGAAGGTGC TGGAGCGCGA CAGCAAGCTG TCGGAGCTGG ACGACCGTGC CGATGCCTTG 
CAGCAGGGTG CCTCGCAGTT TGAGCAGCAG GCGGGCAAGC TCAAGAGGAA ATTCTGGCTT 
CAGAACTTAA AGATGATGAT CATCATGGGC GTGATTGGCC TGGTTGTCGT GGGCATTATT 
GCAAGTAACT TTATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261270.1
CDS	505..882
Translation	MADAAPAGDAPPNAGAPAGEGGDGEIVGGPHNPQQIAAQKRLQQTQAQVDEVVDIMRTNVEKVLERDSKLSELDDRADALQQGASQFEQQAGKLKRKFWLQNLKMMIIMGVIGLVVVGIIASNFM

Target ORF information:

RefSeq Version	NM_001274341.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neuronal synaptobrevin, transcript variant K (nSyb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274341.1

ORF Insert Sequence:

ATGGCGGACG CTGCACCAGC TGGCGATGCA CCACCCAATG CCGGAGCCCC GGCCGGAGAG 
GGCGGCGATG GCGAGATTGT GGGCGGACCA CACAATCCGC AGCAGATCGC GGCACAGAAG 
CGTCTGCAGC AGACGCAGGC GCAGGTCGAT GAGGTCGTGG ACATCATGCG CACGAACGTG 
GAGAAGGTGC TGGAGCGCGA CAGCAAGCTG TCGGAGCTGG ACGACCGTGC CGATGCCTTG 
CAGCAGGGTG CCTCGCAGTT TGAGCAGCAG GCGGGCAAGC TCAAGAGGAA ATTCTGGCTT 
CAGAACTTAA AGATGATGAT CATCATGGGC GTGATTGGCC TGGTTGTCGT GGGCATTATT 
GCAAGTAACT TTATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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nSyb cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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