scf cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	scf
Entrez Gene ID	38145
Full Name	supercoiling factor
Synonyms	BEST:CK02682,CG9148,CK02682,DCB-45,Dmel\CG9148,SCF,anon-EST:Posey14
General protein information	Preferred Names supercoiling factor Names CG9148 gene product from transcript CG9148-RA CG9148-PA CG9148-PB DNA supercoiling factor scf-PA scf-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 61F5-61F5

mRNA and Protein(s)

mRNA	Protein	Name
NM_058044.5	NP_477392.1	supercoiling factor, isoform A
NM_058045.4	NP_477393.1	supercoiling factor, isoform B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CALU	NP_001233521.1
Danio rerio (zebrafish)	calua	NP_001002151.1
Homo sapiens (human)	CALU	NP_001186600.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010392	XP_311555.3
Bos taurus (cattle)	CALU	NP_001029837.1
Drosophila melanogaster (fruit fly)	scf	NP_477392.1
Mus musculus (house mouse)	Calu	NP_908942.1
Rattus norvegicus (Norway rat)	Calu	NP_001029070.1
	CALU	XP_001091525.2
Canis lupus familiaris (dog)	CALU	XP_858778.1
Caenorhabditis elegans (roundworm)	calu-1	NP_001024806.1
Xenopus tropicalis (tropical clawed frog)	calu	NP_998840.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

dRYBP-SCF complex inhibit apoptosis in Drosophila.
Title: A novel dRYBP-SCF complex functions to inhibit apoptosis in Drosophila.

The SCF(Skp2) complex is necessary to ubiquitinate Double-parked during plasmatocyte cell division, ensuring proper cell cycle progression and the generation of a normal population of this essential blood cell type.
Title: Knockdown of SCF(Skp2) function causes double-parked accumulation in the nucleus and DNA re-replication in Drosophila plasmatocytes.

A 54-AA N-terminal fragment of DCB-45 is involved in the interaction with the peptide binding groove of sDQ2, via a pentadecapeptide with high affinity for sDQ2.
Title: Soluble HLA-DQ2 expressed in S2 cells copurifies with a high affinity insect cell derived protein.

The SCF/Slimb ubiquitin ligase limits centrosome amplification through degradation of SAK/PLK4.
Title: The SCF/Slimb ubiquitin ligase limits centrosome amplification through degradation of SAK/PLK4.

SCF plays a role in transcriptional activation via alteration of chromatin structure and contributes to dosage compensation.
Title: DNA supercoiling factor contributes to dosage compensation in Drosophila.

The following scf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the scf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa17128	NM_058044.5 Latest version!	Drosophila melanogaster supercoiling factor (scf), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa17129	NM_058045.4 Latest version!	Drosophila melanogaster supercoiling factor (scf), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

scf ( NM_058044.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477392.1 Drosophila melanogaster supercoiling factor (scf), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa17128
Clone ID Related Accession (Same CDS sequence)	NM_058044.5
Accession Version	NM_058044.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	supercoiling factor, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_058044.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTAGAA CCCGAATTCA GATGCTGCTA CTTGTTGTTG CGCTGACGGC GGTGATAAGT 
GGAGCAAGTG CCTCCAGCAT ACCGGAAGAG CTGCCCCACA ATCCGCTCGA GCACGACCCC 
CTGCACCCCA GGCACTTCGA CGGGGGCGAG CACAATGCCC AGTTTGACCA CGAAGCCTTC 
TTGGGCCCGG ACGAGTCCAA GAAGTTCGAC AGCCTGACAC CCGAGGAGAG CAGGCGGAGA 
TTGGGCGTCA TTGTGGACCG AATCGACGAG AACAAGGACG GGTCAGTCAC ATTGGCGGAG 
CTAAAGAACT GGATCGCCTA CACCCAGAGG CGCTACATCG AAGAAGACGT GGGTCGCGTG 
TGGAAGCAGC ACAATCCGGA CAACAATGAG ACCATAAGCT GGGATTCCTA CATGCAGACT 
GTCTACGGCT TCATGGATGA TCTGAGCCCC GACGAGAAGG AACAGGAGGA AAATGGAGTC 
AGCTACAAGA GCCTGCTGAA ACGCGATCGG TACCGCTGGT CGGTGGCCGA CCAGGACTTG 
GATGACAATC TCACCAAGGA TGAGTTTACC GCCTTCCTGC ACCCCGAGGA CCATCCCAGC 
ATGAAGGGTG TGGTTCTGCG CGAGACCATT ACCGATCTGG ACAAGGACCA CGATGGCAAG 
ATCTCGGTGG ACGAGTACAT TGGCGACATG TACCGATCGA CGGGCGCGGA AGACGAGGAG 
CCCGAGTGGG TGGCTAACGA AAGGGAGGCG TTCTCCACAC ATCGCGACCT GGACAAAGAT 
GGTTATTTGA ACGAGGAGGA AGTGAAGCAG TGGATTGCGC CCCATGACTT CGATCATTCG 
GAGGCGGAGG CCAAGCACCT GCTGTTCGAG GCCGATGCGG ACCACGATGA TAAGTTGACA 
AAGGAGGAAA TACTAGACAA GTACGACGTT TTCGTCGGCT CCCAGGCCAC CGATTTCGGC 
GAGGCCTTGG CCCGCCACGA TGAGTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477392.1
CDS	55..1044
Translation	MARTRIQMLLLVVALTAVISGASASSIPEELPHNPLEHDPLHPRHFDGGEHNAQFDHEAFLGPDESKKFDSLTPEESRRRLGVIVDRIDENKDGSVTLAELKNWIAYTQRRYIEEDVGRVWKQHNPDNNETISWDSYMQTVYGFMDDLSPDEKEQEENGVSYKSLLKRDRYRWSVADQDLDDNLTKDEFTAFLHPEDHPSMKGVVLRETITDLDKDHDGKISVDEYIGDMYRSTGAEDEEPEWVANEREAFSTHRDLDKDGYLNEEEVKQWIAPHDFDHSEAEAKHLLFEADADHDDKLTKEEILDKYDVFVGSQATDFGEALARHDEF

Target ORF information:

RefSeq Version	NM_058044.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster supercoiling factor (scf), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058044.5

ORF Insert Sequence:

ATGGCTAGAA CCCGAATTCA GATGCTGCTA CTTGTTGTTG CGCTGACGGC GGTGATAAGT 
GGAGCAAGTG CCTCCAGCAT ACCGGAAGAG CTGCCCCACA ATCCGCTCGA GCACGACCCC 
CTGCACCCCA GGCACTTCGA CGGGGGCGAG CACAATGCCC AGTTTGACCA CGAAGCCTTC 
TTGGGCCCGG ACGAGTCCAA GAAGTTCGAC AGCCTGACAC CCGAGGAGAG CAGGCGGAGA 
TTGGGCGTCA TTGTGGACCG AATCGACGAG AACAAGGACG GGTCAGTCAC ATTGGCGGAG 
CTAAAGAACT GGATCGCCTA CACCCAGAGG CGCTACATCG AAGAAGACGT GGGTCGCGTG 
TGGAAGCAGC ACAATCCGGA CAACAATGAG ACCATAAGCT GGGATTCCTA CATGCAGACT 
GTCTACGGCT TCATGGATGA TCTGAGCCCC GACGAGAAGG AACAGGAGGA AAATGGAGTC 
AGCTACAAGA GCCTGCTGAA ACGCGATCGG TACCGCTGGT CGGTGGCCGA CCAGGACTTG 
GATGACAATC TCACCAAGGA TGAGTTTACC GCCTTCCTGC ACCCCGAGGA CCATCCCAGC 
ATGAAGGGTG TGGTTCTGCG CGAGACCATT ACCGATCTGG ACAAGGACCA CGATGGCAAG 
ATCTCGGTGG ACGAGTACAT TGGCGACATG TACCGATCGA CGGGCGCGGA AGACGAGGAG 
CCCGAGTGGG TGGCTAACGA AAGGGAGGCG TTCTCCACAC ATCGCGACCT GGACAAAGAT 
GGTTATTTGA ACGAGGAGGA AGTGAAGCAG TGGATTGCGC CCCATGACTT CGATCATTCG 
GAGGCGGAGG CCAAGCACCT GCTGTTCGAG GCCGATGCGG ACCACGATGA TAAGTTGACA 
AAGGAGGAAA TACTAGACAA GTACGACGTT TTCGTCGGCT CCCAGGCCAC CGATTTCGGC 
GAGGCCTTGG CCCGCCACGA TGAGTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scf ( NM_058045.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477393.1 Drosophila melanogaster supercoiling factor (scf), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa17129
Clone ID Related Accession (Same CDS sequence)	NM_058045.4
Accession Version	NM_058045.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	supercoiling factor, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Mar 18, 2004 this sequence version replaced NM_058045.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGACTG TCTACGGCTT CATGGATGAT CTGAGCCCCG ACGAGAAGGA ACAGGAGGAA 
AATGGAGTCA GCTACAAGAG CCTGCTGAAA CGCGATCGGT ACCGCTGGTC GGTGGCCGAC 
CAGGACTTGG ATGACAATCT CACCAAGGAT GAGTTTACCG CCTTCCTGCA CCCCGAGGAC 
CATCCCAGCA TGAAGGGTGT GGTTCTGCGC GAGACCATTA CCGATCTGGA CAAGGACCAC 
GATGGCAAGA TCTCGGTGGA CGAGTACATT GGCGACATGT ACCGATCGAC GGGCGCGGAA 
GACGAGGAGC CCGAGTGGGT GGCTAACGAA AGGGAGGCGT TCTCCACACA TCGCGACCTG 
GACAAAGATG GTTATTTGAA CGAGGAGGAA GTGAAGCAGT GGATTGCGCC CCATGACTTC 
GATCATTCGG AGGCGGAGGC CAAGCACCTG CTGTTCGAGG CCGATGCGGA CCACGATGAT 
AAGTTGACAA AGGAGGAAAT ACTAGACAAG TACGACGTTT TCGTCGGCTC CCAGGCCACC 
GATTTCGGCG AGGCCTTGGC CCGCCACGAT GAGTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477393.1
CDS	252..830
Translation	MQTVYGFMDDLSPDEKEQEENGVSYKSLLKRDRYRWSVADQDLDDNLTKDEFTAFLHPEDHPSMKGVVLRETITDLDKDHDGKISVDEYIGDMYRSTGAEDEEPEWVANEREAFSTHRDLDKDGYLNEEEVKQWIAPHDFDHSEAEAKHLLFEADADHDDKLTKEEILDKYDVFVGSQATDFGEALARHDEF

Target ORF information:

RefSeq Version	NM_058045.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster supercoiling factor (scf), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058045.4

ORF Insert Sequence:

ATGCAGACTG TCTACGGCTT CATGGATGAT CTGAGCCCCG ACGAGAAGGA ACAGGAGGAA 
AATGGAGTCA GCTACAAGAG CCTGCTGAAA CGCGATCGGT ACCGCTGGTC GGTGGCCGAC 
CAGGACTTGG ATGACAATCT CACCAAGGAT GAGTTTACCG CCTTCCTGCA CCCCGAGGAC 
CATCCCAGCA TGAAGGGTGT GGTTCTGCGC GAGACCATTA CCGATCTGGA CAAGGACCAC 
GATGGCAAGA TCTCGGTGGA CGAGTACATT GGCGACATGT ACCGATCGAC GGGCGCGGAA 
GACGAGGAGC CCGAGTGGGT GGCTAACGAA AGGGAGGCGT TCTCCACACA TCGCGACCTG 
GACAAAGATG GTTATTTGAA CGAGGAGGAA GTGAAGCAGT GGATTGCGCC CCATGACTTC 
GATCATTCGG AGGCGGAGGC CAAGCACCTG CTGTTCGAGG CCGATGCGGA CCACGATGAT 
AAGTTGACAA AGGAGGAAAT ACTAGACAAG TACGACGTTT TCGTCGGCTC CCAGGCCACC 
GATTTCGGCG AGGCCTTGGC CCGCCACGAT GAGTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

scf cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報