MED14 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	MED14
Entrez Gene ID	38073
Full Name	Mediator complex subunit 14
Synonyms	CG12031,Dmel\CG12031,Med14,Trap170,dTRAP170,p160
General protein information	Preferred Names Mediator complex subunit 14 Names CG12031 gene product from transcript CG12031-RC CG12031-PA CG12031-PC MED14-PA MED14-PC mediator complex subunit 14
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 61C6-61C6

mRNA and Protein(s)

mRNA	Protein	Name
NM_167835.2	NP_728534.2	mediator complex subunit 14, isoform C
NM_138197.3	NP_612041.2	mediator complex subunit 14, isoform A

Pathways from BioSystems

Homologies

	MED14	XP_001085867.1
Gallus gallus (chicken)	MED14	XP_416776.2
Xenopus tropicalis (tropical clawed frog)	med14	XP_002932839.1
Homo sapiens (human)	MED14	NP_004220.2
Bos taurus (cattle)	MED14	NP_001193960.1
Mus musculus (house mouse)	Med14	NP_001041673.1
Rattus norvegicus (Norway rat)	Med14	NP_001178656.1
Danio rerio (zebrafish)	med14	NP_997930.2
Anopheles gambiae (African malaria mosquito)	MED14_ANOGA	XP_315707.4
Drosophila melanogaster (fruit fly)	MED14	NP_612041.2
Pan troglodytes (chimpanzee)	MED14	XP_003317475.2
Canis lupus familiaris (dog)	MED14	XP_538001.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MED14 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MED14 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa20366	NM_138197.3 Latest version!	Drosophila melanogaster mediator complex subunit 14, transcript variant A (MED14), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa21967	NM_167835.2 Latest version!	Drosophila melanogaster mediator complex subunit 14, transcript variant C (MED14), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MED14 ( NM_138197.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_612041.2 Drosophila melanogaster mediator complex subunit 14, transcript variant A (MED14), mRNA.

Price & Availability↑

CloneID	OFa20366
Clone ID Related Accession (Same CDS sequence)	NM_138197.3
Accession Version	NM_138197.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mediator complex subunit 14, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_138197.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGCCCA CCCCCTTGCC CCTCGAGCAA ATGCCTGGTG TTGGCGGTGG CGGAGGAGGC 
TACCTGCCCG CTGCCCAGGA GGGCGGCCCC CGGATCAACA CCATGTCCAT GTCGGTGCTG 
ATCGACTTCA TCATCCAGCG GACGTATCAT GAACTAACTG TACTGGCTGA ACTATTACCA 
CGCAAAACGG ACATGGAACG CAAGGTGGAG ATCTACAACT ACGCGGCACG CACCCGCCAT 
TTGTTCACCA GGCTGAATGC GCTGGTCAAA TGGGGCAACT CGGTGTCCAA GGTGGACAAA 
TCGTCGCAGA TAATGAGCTT CCTGGACAAG CAGAACATGC TTTTTGTGGA GACCGCCGAC 
ATGCTGGCGC GCATGTCTCG TGAAACTTTG GTTCGTGCTC GATTGCCCAA CTTTCACATA 
CCCGCCGCCG TCGAAGTTCT GACTACTGGC ACATATAATC GTCTACCCAC CTGTATACGC 
GAGCGTATTG TGCCGGCCGA TGCAATTACG CCGGCGGAGA AGAGACAGAC ACTGCTCCGC 
CTGAACCAAG TCATCCAGCA CCGCCTGGTC ACAGGAAAAC TTTTGCCCCA GATGAGAGAA 
TTTCGCATTC GCAACGGACG AGTCACCTTC GAGGTGAAGC ACGAGTTTAG CGTTTCACTG 
ACCGTAATGG GGGATAATCC CACAGTACCT TGGCGGCTGC TGGACATCGA TGTGCTCGTA 
GAGGACAAGG AGACGGGAGA CGGAAAATCG CTGGTGCATC CGCTGCAGGT CAACTACATC 
CATCAGCTCA TCCAGGCACG CCTAGTAGAG AATCCCAATG CTCTTAGCGA GGTCTACAAC 
TGTCTGCACT ACTTCTGCCA ATCCCTGCAG CTGGAGGTTC TGTACACGCA GACCCTGCGT 
TTGAACTACG AAAGACTGGA CGACAACAAT ATAACTGTGG AAGAGTACGT ACCAGGCGTA 
AAACTGACGG TGTCGTATTG GCGTGATCTA AAGTCGGAGC TGGGCTACCG CCTAACCGTT 
CAATCGGATC CCAGCGAGAT CGGGCGTCCG TTGGCAGTGG TCCACGTGCC CTCACTGGGC 
GCCAAGGAGT CTGCCGAAGT GGCGGATCGG GCCGTACGCT CTGAACACCT TTCGATGGAG 
CGCCTAATTG TACATACTGT CTACATACGA TCGGTGTCCA GGCTGAGCGA TCTCAAGCTG 
GAGTTTCAGG CTTTTTTGAA AGATGTTGAC TTCAACCTCC AAGGAACTCC TGCTATTTTG 
ACCGTCCCCG TACTCTCGCC CTGTCTGAGG GCCGAGCAGA TTCACATCAC CATTGACACC 
CACACAGGCA TGTTCCGTTG TCATGTGCCC AAGCATCTGG ACTGTCCGAT AATGGAGGAG 
ATGCAGGACT GCCTCAATGG GGATCGCAGC AAGCTGCCCG CTTTGCTTTC GGAGCTGCGC 
TTTTGGATCA CCCATCGGAG GTGCGACAAA ACCCTACAAC ATCTTCCGGC TACGGCCACC 
GAGACACTTC CATTTCTTGT GCAGCCTGAT CAGGAGATTT TGCAGCCGGG CAGGCACAAG 
ATTTATGTCA AGCTGCACAG GCATCCAAAT ATTGTTTTGG TGGTTCAACT CAAGGAAAAG 
ACTACCATGC CCAACGAAAT GGAATACACC TTTCACCTGG GCTTCGTGGC CTACCAAAAG 
GACGAGCTGG ATGTTATAGA CGACTCCGCC ATGCAGCTGG TGTCTATAGT GGCCCAACCG 
CATTCGGACA TTCCAAAGTG TTACACCAAG CTGATGCGCC TTATTGAGTT TGATACTTTT 
GTTGCCACTC ATGGACCTGG CACTGAGGTG GACGCGGAGG TTTCCCCGCA CAAACGAAAG 
TCGAATGGGG ATTTGCTGGC TCCTCCAGCT AAACAACAAA AGACCATATT TCCTGCCTAC 
TTCATTCCCG AGCTGGCTCA TGTGGTGGCC ATGTGCGATG AAAAGATACC GTTCATGAAC 
CTAGCCCAGA CGCTGTCCAA GCACAATATT CCCCACAGCG GACTGCAGGT GGAAGCGAAT 
GCCACGTCAC TTGTTCTGAA GATACTGGCC TTACCACAGC CAGGAAAAAC AACCTTTGCA 
ACCCAACAAC AGCAGCAACA GGGAGCACCT GCAGTGGCAG GAGAAAATAA GCCAAGTGGT 
ACCTCTGGAC TGCCGAAAAT CGACTCCCAC GTGTGGGACG ACCTAATGCG TCGCGTTCTC 
TCCATATCGA TTCGTTCACA GACCAACAAG AACAGCCAGG TCCGGATCTG GGTAGTGGAG 
TTCGTCTTCT ACAGCACACC GCTGCAAAGT AGCCATCCGA AGGAGCAGGG CAGTCGGCGG 
ACTGTTTACC TCACCTACGA ACAGGCGAAC TACGACTTTT CAAAAACAGT TGAGGATTTG 
CTAAACGACT GGTCGAAAAT CGTGTACCTT TACACGCTGG TTTACGACTT TGCTGAGCAG 
CTGCTAAATA AACGTCTTTC CCTTTGTGAC ATGCTAGTGG TCAAGTCATA CAGCTACATG 
AACCTGCTCC TCGGATACGG ACCCAAAAAA GAGGTGAGCT GCAATATCTA TTGGTCGGTG 
CAATCCCACG GCTTCCGACT GACGTTCGTC GGCGGAATGT CAGCTGTAAA CGGGCACTCA 
ATGATGCGTG ACCAGCTGGC GCAACACTTG AACCAGCAGC ATAGCATCAC CCAAATCGCC 
CAGATTCTCC ATGAAACCTA CAACCCGTTG AGCTCCATTG CCAAGCTGCC GGTGCTGCCC 
TTCTTGGGGA TACCGCGCCC TCAGGTGCCA GTGCTATCGT TTTGCGTGTT GGCACAATCG 
CCGTGCCTGA TAAGGTTGAC ATATCAGGCA GTGTACTGCC TGGAGTTGCG TTTCCGTGCC 
AACAGACTCG TATCCATCCG AGATGGAGCG AGCAGTCGCT TCGAGCGTAA CGTCGTGGAA 
GAGTTCACGC CCATTCAGGG TCTGAAAGCC TTCCTCTCCA AGTACGTGGA CGAGTCGGCG 
GTCTACAGGG GACGGGCCTC CCACGAGGAC GACAATCCGC TGTCACCAAT GGGAATGGAG 
GACAACTTCG GTGGACCGAG CAGTGTTGCG GGGGTAAGTG CTGGTGGATC CTCACCATTT 
TTGGGTACTG GAATGCGGGG ACCGCAGTCG CCGAGAGACA GTGGCCTGCG TTTTCCTGCC 
CCCCACACTC CGCCCTCCAG TTCAAATCCG CACACACCCG CCAGTCCGCA TCCAAGTGCG 
GGGGCAGGTG GCGGCAGTGG ACCCCAGGGA CACGGCAACT TCAACCTGAC GTCACCGCCA 
GCGCCACATA TGCCACATCC GTCGCCAAGC GGCTTGATGC CGTCGTCTCC GCTAAATCCT 
CAGCCCAGTC CCCACATGGT CCATAGTCCT GGACCCAACA CGCTCTACAT GCAGAGCCAC 
CAGGACTCTC CCTTCACCGC CATGTCGCCG GCGAATAACA ATTGGCCCGG ATCCCCCAGC 
ATGCCGCGCC CATCTCCACG ACCCGGACAG AGTCCTGATC ACAAGAGCAC CGGTGGTGGA 
GCAGGAGTGG CCGGTGGAAC GGATAGAGGA GGCTCTCGGG GCACTCTCAA TCGGCCCTGG 
GCTGGAGCAG TACCTACTCT CCTCACCCAT GAAGCTTTGG AGACTCTGTG TCGTCCCAGT 
CCGTATCCGA ACAAGGATAT CAATGTGCCG GACATGAGTC CTCTAGAGCG TTTTCTAGGC 
TGCGTTTATA TGAGAAGGCA ATTGCACAGA AACATTCAGA GCGAGGAGAC GCTTACCGCC 
CTAAACAGTA CAGAACCAGG AGTGGTGCTC TTCAAGGTCG ATGGACTGCA GTGCCAGGTG 
GTGCTGAATC AGATGCACAT GCAGACGCTG CACCTCAAAG TCTCTCAGCT GCCGCCCATG 
CCCGACAAGC ATCCGCCGCC GTTCCAGCTT AGCCAGGACG ATCTGCTGGT TATAGAGCAG 
TACTTTGACA CCAGAGTGGC AGCTCCACCA TACCGACCCA ATTCCCTGCA CAGCATCTGC 
CGGCTGTTGA ACCTGCCCGC CCAGGTACTT AAGGATTTCG TACAGATCAT GCGTTTGGAT 
TTGAAGCCGG AGCTGGGTGG CGACCAGTTG AAATGGACGG TGCAGATATG TTTGCGCATG 
CCGCCCTCGG CGGTTCCCAT TGTGCCCAGT GGCAATGCAT GCGTGGTTAT GGGTCGCATG 
AAGATACTGT TCTTCCTGCA AATCACGAGG ATACCCTACG GCGCGGTCAT AGGCGTGGGC 
AAGGACTGGA AGGACAGCCC CTCTTTGGTG TTGCCCATTG TGTACGACAT TCAGACGAAC 
GTCACCCAGC TGGCGGAACG CACAGGACAA GTGATCTCGC CCACAATGAC CGCTGCTAGC 
ACTCTTCTTC GACGCTTTGC CGAGTTCAAT GCGCAACAGA ATCAGTGTAC GCTCTTCCCA 
GCCATTACGG ATCTTCTGAC GAACCTCCAG CTGGCTGCCG AGATGCCACA GCCCCCACCC 
AACCAATCCA TAGGACCTCC CGTAGGCGTG GGTGTCGGAG TGGGATCCAG TCCGAATCCG 
ATGATGCCCA TGCAGCAGCT GCCGCAGCAA GTGGGACCGC AGGGCCCAGT GGGTCCCGGC 
GGCTACCCGC AGATGGGCCC CAATCCCGGT GGCCCGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_612041.2
CDS	181..4842
Translation	MAPTPLPLEQMPGVGGGGGGYLPAAQEGGPRINTMSMSVLIDFIIQRTYHELTVLAELLPRKTDMERKVEIYNYAARTRHLFTRLNALVKWGNSVSKVDKSSQIMSFLDKQNMLFVETADMLARMSRETLVRARLPNFHIPAAVEVLTTGTYNRLPTCIRERIVPADAITPAEKRQTLLRLNQVIQHRLVTGKLLPQMREFRIRNGRVTFEVKHEFSVSLTVMGDNPTVPWRLLDIDVLVEDKETGDGKSLVHPLQVNYIHQLIQARLVENPNALSEVYNCLHYFCQSLQLEVLYTQTLRLNYERLDDNNITVEEYVPGVKLTVSYWRDLKSELGYRLTVQSDPSEIGRPLAVVHVPSLGAKESAEVADRAVRSEHLSMERLIVHTVYIRSVSRLSDLKLEFQAFLKDVDFNLQGTPAILTVPVLSPCLRAEQIHITIDTHTGMFRCHVPKHLDCPIMEEMQDCLNGDRSKLPALLSELRFWITHRRCDKTLQHLPATATETLPFLVQPDQEILQPGRHKIYVKLHRHPNIVLVVQLKEKTTMPNEMEYTFHLGFVAYQKDELDVIDDSAMQLVSIVAQPHSDIPKCYTKLMRLIEFDTFVATHGPGTEVDAEVSPHKRKSNGDLLAPPAKQQKTIFPAYFIPELAHVVAMCDEKIPFMNLAQTLSKHNIPHSGLQVEANATSLVLKILALPQPGKTTFATQQQQQQGAPAVAGENKPSGTSGLPKIDSHVWDDLMRRVLSISIRSQTNKNSQVRIWVVEFVFYSTPLQSSHPKEQGSRRTVYLTYEQANYDFSKTVEDLLNDWSKIVYLYTLVYDFAEQLLNKRLSLCDMLVVKSYSYMNLLLGYGPKKEVSCNIYWSVQSHGFRLTFVGGMSAVNGHSMMRDQLAQHLNQQHSITQIAQILHETYNPLSSIAKLPVLPFLGIPRPQVPVLSFCVLAQSPCLIRLTYQAVYCLELRFRANRLVSIRDGASSRFERNVVEEFTPIQGLKAFLSKYVDESAVYRGRASHEDDNPLSPMGMEDNFGGPSSVAGVSAGGSSPFLGTGMRGPQSPRDSGLRFPAPHTPPSSSNPHTPASPHPSAGAGGGSGPQGHGNFNLTSPPAPHMPHPSPSGLMPSSPLNPQPSPHMVHSPGPNTLYMQSHQDSPFTAMSPANNNWPGSPSMPRPSPRPGQSPDHKSTGGGAGVAGGTDRGGSRGTLNRPWAGAVPTLLTHEALETLCRPSPYPNKDINVPDMSPLERFLGCVYMRRQLHRNIQSEETLTALNSTEPGVVLFKVDGLQCQVVLNQMHMQTLHLKVSQLPPMPDKHPPPFQLSQDDLLVIEQYFDTRVAAPPYRPNSLHSICRLLNLPAQVLKDFVQIMRLDLKPELGGDQLKWTVQICLRMPPSAVPIVPSGNACVVMGRMKILFFLQITRIPYGAVIGVGKDWKDSPSLVLPIVYDIQTNVTQLAERTGQVISPTMTAASTLLRRFAEFNAQQNQCTLFPAITDLLTNLQLAAEMPQPPPNQSIGPPVGVGVGVGSSPNPMMPMQQLPQQVGPQGPVGPGGYPQMGPNPGGPQ

Target ORF information:

RefSeq Version	NM_138197.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mediator complex subunit 14, transcript variant A (MED14), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138197.3

ORF Insert Sequence:

ATGGCGCCCA CCCCCTTGCC CCTCGAGCAA ATGCCTGGTG TTGGCGGTGG CGGAGGAGGC 
TACCTGCCCG CTGCCCAGGA GGGCGGCCCC CGGATCAACA CCATGTCCAT GTCGGTGCTG 
ATCGACTTCA TCATCCAGCG GACGTATCAT GAACTAACTG TACTGGCTGA ACTATTACCA 
CGCAAAACGG ACATGGAACG CAAGGTGGAG ATCTACAACT ACGCGGCACG CACCCGCCAT 
TTGTTCACCA GGCTGAATGC GCTGGTCAAA TGGGGCAACT CGGTGTCCAA GGTGGACAAA 
TCGTCGCAGA TAATGAGCTT CCTGGACAAG CAGAACATGC TTTTTGTGGA GACCGCCGAC 
ATGCTGGCGC GCATGTCTCG TGAAACTTTG GTTCGTGCTC GATTGCCCAA CTTTCACATA 
CCCGCCGCCG TCGAAGTTCT GACTACTGGC ACATATAATC GTCTACCCAC CTGTATACGC 
GAGCGTATTG TGCCGGCCGA TGCAATTACG CCGGCGGAGA AGAGACAGAC ACTGCTCCGC 
CTGAACCAAG TCATCCAGCA CCGCCTGGTC ACAGGAAAAC TTTTGCCCCA GATGAGAGAA 
TTTCGCATTC GCAACGGACG AGTCACCTTC GAGGTGAAGC ACGAGTTTAG CGTTTCACTG 
ACCGTAATGG GGGATAATCC CACAGTACCT TGGCGGCTGC TGGACATCGA TGTGCTCGTA 
GAGGACAAGG AGACGGGAGA CGGAAAATCG CTGGTGCATC CGCTGCAGGT CAACTACATC 
CATCAGCTCA TCCAGGCACG CCTAGTAGAG AATCCCAATG CTCTTAGCGA GGTCTACAAC 
TGTCTGCACT ACTTCTGCCA ATCCCTGCAG CTGGAGGTTC TGTACACGCA GACCCTGCGT 
TTGAACTACG AAAGACTGGA CGACAACAAT ATAACTGTGG AAGAGTACGT ACCAGGCGTA 
AAACTGACGG TGTCGTATTG GCGTGATCTA AAGTCGGAGC TGGGCTACCG CCTAACCGTT 
CAATCGGATC CCAGCGAGAT CGGGCGTCCG TTGGCAGTGG TCCACGTGCC CTCACTGGGC 
GCCAAGGAGT CTGCCGAAGT GGCGGATCGG GCCGTACGCT CTGAACACCT TTCGATGGAG 
CGCCTAATTG TACATACTGT CTACATACGA TCGGTGTCCA GGCTGAGCGA TCTCAAGCTG 
GAGTTTCAGG CTTTTTTGAA AGATGTTGAC TTCAACCTCC AAGGAACTCC TGCTATTTTG 
ACCGTCCCCG TACTCTCGCC CTGTCTGAGG GCCGAGCAGA TTCACATCAC CATTGACACC 
CACACAGGCA TGTTCCGTTG TCATGTGCCC AAGCATCTGG ACTGTCCGAT AATGGAGGAG 
ATGCAGGACT GCCTCAATGG GGATCGCAGC AAGCTGCCCG CTTTGCTTTC GGAGCTGCGC 
TTTTGGATCA CCCATCGGAG GTGCGACAAA ACCCTACAAC ATCTTCCGGC TACGGCCACC 
GAGACACTTC CATTTCTTGT GCAGCCTGAT CAGGAGATTT TGCAGCCGGG CAGGCACAAG 
ATTTATGTCA AGCTGCACAG GCATCCAAAT ATTGTTTTGG TGGTTCAACT CAAGGAAAAG 
ACTACCATGC CCAACGAAAT GGAATACACC TTTCACCTGG GCTTCGTGGC CTACCAAAAG 
GACGAGCTGG ATGTTATAGA CGACTCCGCC ATGCAGCTGG TGTCTATAGT GGCCCAACCG 
CATTCGGACA TTCCAAAGTG TTACACCAAG CTGATGCGCC TTATTGAGTT TGATACTTTT 
GTTGCCACTC ATGGACCTGG CACTGAGGTG GACGCGGAGG TTTCCCCGCA CAAACGAAAG 
TCGAATGGGG ATTTGCTGGC TCCTCCAGCT AAACAACAAA AGACCATATT TCCTGCCTAC 
TTCATTCCCG AGCTGGCTCA TGTGGTGGCC ATGTGCGATG AAAAGATACC GTTCATGAAC 
CTAGCCCAGA CGCTGTCCAA GCACAATATT CCCCACAGCG GACTGCAGGT GGAAGCGAAT 
GCCACGTCAC TTGTTCTGAA GATACTGGCC TTACCACAGC CAGGAAAAAC AACCTTTGCA 
ACCCAACAAC AGCAGCAACA GGGAGCACCT GCAGTGGCAG GAGAAAATAA GCCAAGTGGT 
ACCTCTGGAC TGCCGAAAAT CGACTCCCAC GTGTGGGACG ACCTAATGCG TCGCGTTCTC 
TCCATATCGA TTCGTTCACA GACCAACAAG AACAGCCAGG TCCGGATCTG GGTAGTGGAG 
TTCGTCTTCT ACAGCACACC GCTGCAAAGT AGCCATCCGA AGGAGCAGGG CAGTCGGCGG 
ACTGTTTACC TCACCTACGA ACAGGCGAAC TACGACTTTT CAAAAACAGT TGAGGATTTG 
CTAAACGACT GGTCGAAAAT CGTGTACCTT TACACGCTGG TTTACGACTT TGCTGAGCAG 
CTGCTAAATA AACGTCTTTC CCTTTGTGAC ATGCTAGTGG TCAAGTCATA CAGCTACATG 
AACCTGCTCC TCGGATACGG ACCCAAAAAA GAGGTGAGCT GCAATATCTA TTGGTCGGTG 
CAATCCCACG GCTTCCGACT GACGTTCGTC GGCGGAATGT CAGCTGTAAA CGGGCACTCA 
ATGATGCGTG ACCAGCTGGC GCAACACTTG AACCAGCAGC ATAGCATCAC CCAAATCGCC 
CAGATTCTCC ATGAAACCTA CAACCCGTTG AGCTCCATTG CCAAGCTGCC GGTGCTGCCC 
TTCTTGGGGA TACCGCGCCC TCAGGTGCCA GTGCTATCGT TTTGCGTGTT GGCACAATCG 
CCGTGCCTGA TAAGGTTGAC ATATCAGGCA GTGTACTGCC TGGAGTTGCG TTTCCGTGCC 
AACAGACTCG TATCCATCCG AGATGGAGCG AGCAGTCGCT TCGAGCGTAA CGTCGTGGAA 
GAGTTCACGC CCATTCAGGG TCTGAAAGCC TTCCTCTCCA AGTACGTGGA CGAGTCGGCG 
GTCTACAGGG GACGGGCCTC CCACGAGGAC GACAATCCGC TGTCACCAAT GGGAATGGAG 
GACAACTTCG GTGGACCGAG CAGTGTTGCG GGGGTAAGTG CTGGTGGATC CTCACCATTT 
TTGGGTACTG GAATGCGGGG ACCGCAGTCG CCGAGAGACA GTGGCCTGCG TTTTCCTGCC 
CCCCACACTC CGCCCTCCAG TTCAAATCCG CACACACCCG CCAGTCCGCA TCCAAGTGCG 
GGGGCAGGTG GCGGCAGTGG ACCCCAGGGA CACGGCAACT TCAACCTGAC GTCACCGCCA 
GCGCCACATA TGCCACATCC GTCGCCAAGC GGCTTGATGC CGTCGTCTCC GCTAAATCCT 
CAGCCCAGTC CCCACATGGT CCATAGTCCT GGACCCAACA CGCTCTACAT GCAGAGCCAC 
CAGGACTCTC CCTTCACCGC CATGTCGCCG GCGAATAACA ATTGGCCCGG ATCCCCCAGC 
ATGCCGCGCC CATCTCCACG ACCCGGACAG AGTCCTGATC ACAAGAGCAC CGGTGGTGGA 
GCAGGAGTGG CCGGTGGAAC GGATAGAGGA GGCTCTCGGG GCACTCTCAA TCGGCCCTGG 
GCTGGAGCAG TACCTACTCT CCTCACCCAT GAAGCTTTGG AGACTCTGTG TCGTCCCAGT 
CCGTATCCGA ACAAGGATAT CAATGTGCCG GACATGAGTC CTCTAGAGCG TTTTCTAGGC 
TGCGTTTATA TGAGAAGGCA ATTGCACAGA AACATTCAGA GCGAGGAGAC GCTTACCGCC 
CTAAACAGTA CAGAACCAGG AGTGGTGCTC TTCAAGGTCG ATGGACTGCA GTGCCAGGTG 
GTGCTGAATC AGATGCACAT GCAGACGCTG CACCTCAAAG TCTCTCAGCT GCCGCCCATG 
CCCGACAAGC ATCCGCCGCC GTTCCAGCTT AGCCAGGACG ATCTGCTGGT TATAGAGCAG 
TACTTTGACA CCAGAGTGGC AGCTCCACCA TACCGACCCA ATTCCCTGCA CAGCATCTGC 
CGGCTGTTGA ACCTGCCCGC CCAGGTACTT AAGGATTTCG TACAGATCAT GCGTTTGGAT 
TTGAAGCCGG AGCTGGGTGG CGACCAGTTG AAATGGACGG TGCAGATATG TTTGCGCATG 
CCGCCCTCGG CGGTTCCCAT TGTGCCCAGT GGCAATGCAT GCGTGGTTAT GGGTCGCATG 
AAGATACTGT TCTTCCTGCA AATCACGAGG ATACCCTACG GCGCGGTCAT AGGCGTGGGC 
AAGGACTGGA AGGACAGCCC CTCTTTGGTG TTGCCCATTG TGTACGACAT TCAGACGAAC 
GTCACCCAGC TGGCGGAACG CACAGGACAA GTGATCTCGC CCACAATGAC CGCTGCTAGC 
ACTCTTCTTC GACGCTTTGC CGAGTTCAAT GCGCAACAGA ATCAGTGTAC GCTCTTCCCA 
GCCATTACGG ATCTTCTGAC GAACCTCCAG CTGGCTGCCG AGATGCCACA GCCCCCACCC 
AACCAATCCA TAGGACCTCC CGTAGGCGTG GGTGTCGGAG TGGGATCCAG TCCGAATCCG 
ATGATGCCCA TGCAGCAGCT GCCGCAGCAA GTGGGACCGC AGGGCCCAGT GGGTCCCGGC 
GGCTACCCGC AGATGGGCCC CAATCCCGGT GGCCCGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED14 ( NM_167835.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728534.2 Drosophila melanogaster mediator complex subunit 14, transcript variant C (MED14), mRNA.

Price & Availability↑

CloneID	OFa21967
Clone ID Related Accession (Same CDS sequence)	NM_167835.2
Accession Version	NM_167835.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mediator complex subunit 14, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_167835.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGCCCA CCCCCTTGCC CCTCGAGCAA ATGCCTGGTG TTGGCGGTGG CGGAGGAGGC 
TACCTGCCCG CTGCCCAGGA GGGCGGCCCC CGGATCAACA CCATGTCCAT GTCGGTGCTG 
ATCGACTTCA TCATCCAGCG GACGTATCAT GAACTAACTG TACTGGCTGA ACTATTACCA 
CGCAAAACGG ACATGGAACG CAAGGTGGAG ATCTACAACT ACGCGGCACG CACCCGCCAT 
TTGTTCACCA GGCTGAATGC GCTGGTCAAA TGGGGCAACT CGGTGTCCAA GGTGGACAAA 
TCGTCGCAGA TAATGAGCTT CCTGGACAAG CAGAACATGC TTTTTGTGGA GACCGCCGAC 
ATGCTGGCGC GCATGTCTCG TGAAACTTTG GTTCGTGCTC GATTGCCCAA CTTTCACATA 
CCCGCCGCCG TCGAAGTTCT GACTACTGGC ACATATAATC GTCTACCCAC CTGTATACGC 
GAGCGTATTG TGCCGGCCGA TGCAATTACG CCGGCGGAGA AGAGACAGAC ACTGCTCCGC 
CTGAACCAAG TCATCCAGCA CCGCCTGGTC ACAGGAAAAC TTTTGCCCCA GATGAGAGAA 
TTTCGCATTC GCAACGGACG AGTCACCTTC GAGGTGAAGC ACGAGTTTAG CGTTTCACTG 
ACCGTAATGG GGGATAATCC CACAGTACCT TGGCGGCTGC TGGACATCGA TGTGCTCGTA 
GAGGACAAGG AGACGGGAGA CGGAAAATCG CTGGTGCATC CGCTGCAGGT CAACTACATC 
CATCAGCTCA TCCAGGCACG CCTAGTAGAG AATCCCAATG CTCTTAGCGA GGTCTACAAC 
TGTCTGCACT ACTTCTGCCA ATCCCTGCAG CTGGAGGTTC TGTACACGCA GACCCTGCGT 
TTGAACTACG AAAGACTGGA CGACAACAAT ATAACTGTGG AAGAGTACGT ACCAGGCGTA 
AAACTGACGG TGTCGTATTG GCGTGATCTA AAGTCGGAGC TGGGCTACCG CCTAACCGTT 
CAATCGGATC CCAGCGAGAT CGGGCGTCCG TTGGCAGTGG TCCACGTGCC CTCACTGGGC 
GCCAAGGAGT CTGCCGAAGT GGCGGATCGG GCCGTACGCT CTGAACACCT TTCGATGGAG 
CGCCTAATTG TACATACTGT CTACATACGA TCGGTGTCCA GGCTGAGCGA TCTCAAGCTG 
GAGTTTCAGG CTTTTTTGAA AGATGTTGAC TGTGAGTTCA ACCTCCAAGG AACTCCTGCT 
ATTTTGACCG TCCCCGTACT CTCGCCCTGT CTGAGGGCCG AGCAGATTCA CATCACCATT 
GACACCCACA CAGGCATGTT CCGTTGTCAT GTGCCCAAGC ATCTGGACTG TCCGATAATG 
GAGGAGATGC AGGACTGCCT CAATGGGGAT CGCAGCAAGC TGCCCGCTTT GCTTTCGGAG 
CTGCGCTTTT GGATCACCCA TCGGAGGTGC GACAAAACCC TACAACATCT TCCGGCTACG 
GCCACCGAGA CACTTCCATT TCTTGTGCAG CCTGATCAGG AGATTTTGCA GCCGGGCAGG 
CACAAGATTT ATGTCAAGCT GCACAGGCAT CCAAATATTG TTTTGGTGGT TCAACTCAAG 
GAAAAGACTA CCATGCCCAA CGAAATGGAA TACACCTTTC ACCTGGGCTT CGTGGCCTAC 
CAAAAGGACG AGCTGGATGT TATAGACGAC TCCGCCATGC AGCTGGTGTC TATAGTGGCC 
CAACCGCATT CGGACATTCC AAAGTGTTAC ACCAAGCTGA TGCGCCTTAT TGAGTTTGAT 
ACTTTTGTTG CCACTCATGG ACCTGGCACT GAGGTGGACG CGGAGGTTTC CCCGCACAAA 
CGAAAGTCGA ATGGGGATTT GCTGGCTCCT CCAGCTAAAC AACAAAAGAC CATATTTCCT 
GCCTACTTCA TTCCCGAGCT GGCTCATGTG GTGGCCATGT GCGATGAAAA GATACCGTTC 
ATGAACCTAG CCCAGACGCT GTCCAAGCAC AATATTCCCC ACAGCGGACT GCAGGTGGAA 
GCGAATGCCA CGTCACTTGT TCTGAAGATA CTGGCCTTAC CACAGCCAGG AAAAACAACC 
TTTGCAACCC AACAACAGCA GCAACAGGGA GCACCTGCAG TGGCAGGAGA AAATAAGCCA 
AGTGGTACCT CTGGACTGCC GAAAATCGAC TCCCACGTGT GGGACGACCT AATGCGTCGC 
GTTCTCTCCA TATCGATTCG TTCACAGACC AACAAGAACA GCCAGGTCCG GATCTGGGTA 
GTGGAGTTCG TCTTCTACAG CACACCGCTG CAAAGTAGCC ATCCGAAGGA GCAGGGCAGT 
CGGCGGACTG TTTACCTCAC CTACGAACAG GCGAACTACG ACTTTTCAAA AACAGTTGAG 
GATTTGCTAA ACGACTGGTC GAAAATCGTG TACCTTTACA CGCTGGTTTA CGACTTTGCT 
GAGCAGCTGC TAAATAAACG TCTTTCCCTT TGTGACATGC TAGTGGTCAA GTCATACAGC 
TACATGAACC TGCTCCTCGG ATACGGACCC AAAAAAGAGG TGAGCTGCAA TATCTATTGG 
TCGGTGCAAT CCCACGGCTT CCGACTGACG TTCGTCGGCG GAATGTCAGC TGTAAACGGG 
CACTCAATGA TGCGTGACCA GCTGGCGCAA CACTTGAACC AGCAGCATAG CATCACCCAA 
ATCGCCCAGA TTCTCCATGA AACCTACAAC CCGTTGAGCT CCATTGCCAA GCTGCCGGTG 
CTGCCCTTCT TGGGGATACC GCGCCCTCAG GTGCCAGTGC TATCGTTTTG CGTGTTGGCA 
CAATCGCCGT GCCTGATAAG GTTGACATAT CAGGCAGTGT ACTGCCTGGA GTTGCGTTTC 
CGTGCCAACA GACTCGTATC CATCCGAGAT GGAGCGAGCA GTCGCTTCGA GCGTAACGTC 
GTGGAAGAGT TCACGCCCAT TCAGGGTCTG AAAGCCTTCC TCTCCAAGTA CGTGGACGAG 
TCGGCGGTCT ACAGGGGACG GGCCTCCCAC GAGGACGACA ATCCGCTGTC ACCAATGGGA 
ATGGAGGACA ACTTCGGTGG ACCGAGCAGT GTTGCGGGGG TAAGTGCTGG TGGATCCTCA 
CCATTTTTGG GTACTGGAAT GCGGGGACCG CAGTCGCCGA GAGACAGTGG CCTGCGTTTT 
CCTGCCCCCC ACACTCCGCC CTCCAGTTCA AATCCGCACA CACCCGCCAG TCCGCATCCA 
AGTGCGGGGG CAGGTGGCGG CAGTGGACCC CAGGGACACG GCAACTTCAA CCTGACGTCA 
CCGCCAGCGC CACATATGCC ACATCCGTCG CCAAGCGGCT TGATGCCGTC GTCTCCGCTA 
AATCCTCAGC CCAGTCCCCA CATGGTCCAT AGTCCTGGAC CCAACACGCT CTACATGCAG 
AGCCACCAGG ACTCTCCCTT CACCGCCATG TCGCCGGCGA ATAACAATTG GCCCGGATCC 
CCCAGCATGC CGCGCCCATC TCCACGACCC GGACAGAGTC CTGATCACAA GAGCACCGGT 
GGTGGAGCAG GAGTGGCCGG TGGAACGGAT AGAGGAGGCT CTCGGGGCAC TCTCAATCGG 
CCCTGGGCTG GAGCAGTACC TACTCTCCTC ACCCATGAAG CTTTGGAGAC TCTGTGTCGT 
CCCAGTCCGT ATCCGAACAA GGATATCAAT GTGCCGGACA TGAGTCCTCT AGAGCGTTTT 
CTAGGCTGCG TTTATATGAG AAGGCAATTG CACAGAAACA TTCAGAGCGA GGAGACGCTT 
ACCGCCCTAA ACAGTACAGA ACCAGGAGTG GTGCTCTTCA AGGTCGATGG ACTGCAGTGC 
CAGGTGGTGC TGAATCAGAT GCACATGCAG ACGCTGCACC TCAAAGTCTC TCAGCTGCCG 
CCCATGCCCG ACAAGCATCC GCCGCCGTTC CAGCTTAGCC AGGACGATCT GCTGGTTATA 
GAGCAGTACT TTGACACCAG AGTGGCAGCT CCACCATACC GACCCAATTC CCTGCACAGC 
ATCTGCCGGC TGTTGAACCT GCCCGCCCAG GTACTTAAGG ATTTCGTACA GATCATGCGT 
TTGGATTTGA AGCCGGAGCT GGGTGGCGAC CAGTTGAAAT GGACGGTGCA GATATGTTTG 
CGCATGCCGC CCTCGGCGGT TCCCATTGTG CCCAGTGGCA ATGCATGCGT GGTTATGGGT 
CGCATGAAGA TACTGTTCTT CCTGCAAATC ACGAGGATAC CCTACGGCGC GGTCATAGGC 
GTGGGCAAGG ACTGGAAGGA CAGCCCCTCT TTGGTGTTGC CCATTGTGTA CGACATTCAG 
ACGAACGTCA CCCAGCTGGC GGAACGCACA GGACAAGTGA TCTCGCCCAC AATGACCGCT 
GCTAGCACTC TTCTTCGACG CTTTGCCGAG TTCAATGCGC AACAGAATCA GTGTACGCTC 
TTCCCAGCCA TTACGGATCT TCTGACGAAC CTCCAGCTGG CTGCCGAGAT GCCACAGCCC 
CCACCCAACC AATCCATAGG ACCTCCCGTA GGCGTGGGTG TCGGAGTGGG ATCCAGTCCG 
AATCCGATGA TGCCCATGCA GCAGCTGCCG CAGCAAGTGG GACCGCAGGG CCCAGTGGGT 
CCCGGCGGCT ACCCGCAGAT GGGCCCCAAT CCCGGTGGCC CGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728534.2
CDS	181..4848
Translation	MAPTPLPLEQMPGVGGGGGGYLPAAQEGGPRINTMSMSVLIDFIIQRTYHELTVLAELLPRKTDMERKVEIYNYAARTRHLFTRLNALVKWGNSVSKVDKSSQIMSFLDKQNMLFVETADMLARMSRETLVRARLPNFHIPAAVEVLTTGTYNRLPTCIRERIVPADAITPAEKRQTLLRLNQVIQHRLVTGKLLPQMREFRIRNGRVTFEVKHEFSVSLTVMGDNPTVPWRLLDIDVLVEDKETGDGKSLVHPLQVNYIHQLIQARLVENPNALSEVYNCLHYFCQSLQLEVLYTQTLRLNYERLDDNNITVEEYVPGVKLTVSYWRDLKSELGYRLTVQSDPSEIGRPLAVVHVPSLGAKESAEVADRAVRSEHLSMERLIVHTVYIRSVSRLSDLKLEFQAFLKDVDCEFNLQGTPAILTVPVLSPCLRAEQIHITIDTHTGMFRCHVPKHLDCPIMEEMQDCLNGDRSKLPALLSELRFWITHRRCDKTLQHLPATATETLPFLVQPDQEILQPGRHKIYVKLHRHPNIVLVVQLKEKTTMPNEMEYTFHLGFVAYQKDELDVIDDSAMQLVSIVAQPHSDIPKCYTKLMRLIEFDTFVATHGPGTEVDAEVSPHKRKSNGDLLAPPAKQQKTIFPAYFIPELAHVVAMCDEKIPFMNLAQTLSKHNIPHSGLQVEANATSLVLKILALPQPGKTTFATQQQQQQGAPAVAGENKPSGTSGLPKIDSHVWDDLMRRVLSISIRSQTNKNSQVRIWVVEFVFYSTPLQSSHPKEQGSRRTVYLTYEQANYDFSKTVEDLLNDWSKIVYLYTLVYDFAEQLLNKRLSLCDMLVVKSYSYMNLLLGYGPKKEVSCNIYWSVQSHGFRLTFVGGMSAVNGHSMMRDQLAQHLNQQHSITQIAQILHETYNPLSSIAKLPVLPFLGIPRPQVPVLSFCVLAQSPCLIRLTYQAVYCLELRFRANRLVSIRDGASSRFERNVVEEFTPIQGLKAFLSKYVDESAVYRGRASHEDDNPLSPMGMEDNFGGPSSVAGVSAGGSSPFLGTGMRGPQSPRDSGLRFPAPHTPPSSSNPHTPASPHPSAGAGGGSGPQGHGNFNLTSPPAPHMPHPSPSGLMPSSPLNPQPSPHMVHSPGPNTLYMQSHQDSPFTAMSPANNNWPGSPSMPRPSPRPGQSPDHKSTGGGAGVAGGTDRGGSRGTLNRPWAGAVPTLLTHEALETLCRPSPYPNKDINVPDMSPLERFLGCVYMRRQLHRNIQSEETLTALNSTEPGVVLFKVDGLQCQVVLNQMHMQTLHLKVSQLPPMPDKHPPPFQLSQDDLLVIEQYFDTRVAAPPYRPNSLHSICRLLNLPAQVLKDFVQIMRLDLKPELGGDQLKWTVQICLRMPPSAVPIVPSGNACVVMGRMKILFFLQITRIPYGAVIGVGKDWKDSPSLVLPIVYDIQTNVTQLAERTGQVISPTMTAASTLLRRFAEFNAQQNQCTLFPAITDLLTNLQLAAEMPQPPPNQSIGPPVGVGVGVGSSPNPMMPMQQLPQQVGPQGPVGPGGYPQMGPNPGGPQ

Target ORF information:

RefSeq Version	NM_167835.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mediator complex subunit 14, transcript variant C (MED14), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167835.2

ORF Insert Sequence:

ATGGCGCCCA CCCCCTTGCC CCTCGAGCAA ATGCCTGGTG TTGGCGGTGG CGGAGGAGGC 
TACCTGCCCG CTGCCCAGGA GGGCGGCCCC CGGATCAACA CCATGTCCAT GTCGGTGCTG 
ATCGACTTCA TCATCCAGCG GACGTATCAT GAACTAACTG TACTGGCTGA ACTATTACCA 
CGCAAAACGG ACATGGAACG CAAGGTGGAG ATCTACAACT ACGCGGCACG CACCCGCCAT 
TTGTTCACCA GGCTGAATGC GCTGGTCAAA TGGGGCAACT CGGTGTCCAA GGTGGACAAA 
TCGTCGCAGA TAATGAGCTT CCTGGACAAG CAGAACATGC TTTTTGTGGA GACCGCCGAC 
ATGCTGGCGC GCATGTCTCG TGAAACTTTG GTTCGTGCTC GATTGCCCAA CTTTCACATA 
CCCGCCGCCG TCGAAGTTCT GACTACTGGC ACATATAATC GTCTACCCAC CTGTATACGC 
GAGCGTATTG TGCCGGCCGA TGCAATTACG CCGGCGGAGA AGAGACAGAC ACTGCTCCGC 
CTGAACCAAG TCATCCAGCA CCGCCTGGTC ACAGGAAAAC TTTTGCCCCA GATGAGAGAA 
TTTCGCATTC GCAACGGACG AGTCACCTTC GAGGTGAAGC ACGAGTTTAG CGTTTCACTG 
ACCGTAATGG GGGATAATCC CACAGTACCT TGGCGGCTGC TGGACATCGA TGTGCTCGTA 
GAGGACAAGG AGACGGGAGA CGGAAAATCG CTGGTGCATC CGCTGCAGGT CAACTACATC 
CATCAGCTCA TCCAGGCACG CCTAGTAGAG AATCCCAATG CTCTTAGCGA GGTCTACAAC 
TGTCTGCACT ACTTCTGCCA ATCCCTGCAG CTGGAGGTTC TGTACACGCA GACCCTGCGT 
TTGAACTACG AAAGACTGGA CGACAACAAT ATAACTGTGG AAGAGTACGT ACCAGGCGTA 
AAACTGACGG TGTCGTATTG GCGTGATCTA AAGTCGGAGC TGGGCTACCG CCTAACCGTT 
CAATCGGATC CCAGCGAGAT CGGGCGTCCG TTGGCAGTGG TCCACGTGCC CTCACTGGGC 
GCCAAGGAGT CTGCCGAAGT GGCGGATCGG GCCGTACGCT CTGAACACCT TTCGATGGAG 
CGCCTAATTG TACATACTGT CTACATACGA TCGGTGTCCA GGCTGAGCGA TCTCAAGCTG 
GAGTTTCAGG CTTTTTTGAA AGATGTTGAC TGTGAGTTCA ACCTCCAAGG AACTCCTGCT 
ATTTTGACCG TCCCCGTACT CTCGCCCTGT CTGAGGGCCG AGCAGATTCA CATCACCATT 
GACACCCACA CAGGCATGTT CCGTTGTCAT GTGCCCAAGC ATCTGGACTG TCCGATAATG 
GAGGAGATGC AGGACTGCCT CAATGGGGAT CGCAGCAAGC TGCCCGCTTT GCTTTCGGAG 
CTGCGCTTTT GGATCACCCA TCGGAGGTGC GACAAAACCC TACAACATCT TCCGGCTACG 
GCCACCGAGA CACTTCCATT TCTTGTGCAG CCTGATCAGG AGATTTTGCA GCCGGGCAGG 
CACAAGATTT ATGTCAAGCT GCACAGGCAT CCAAATATTG TTTTGGTGGT TCAACTCAAG 
GAAAAGACTA CCATGCCCAA CGAAATGGAA TACACCTTTC ACCTGGGCTT CGTGGCCTAC 
CAAAAGGACG AGCTGGATGT TATAGACGAC TCCGCCATGC AGCTGGTGTC TATAGTGGCC 
CAACCGCATT CGGACATTCC AAAGTGTTAC ACCAAGCTGA TGCGCCTTAT TGAGTTTGAT 
ACTTTTGTTG CCACTCATGG ACCTGGCACT GAGGTGGACG CGGAGGTTTC CCCGCACAAA 
CGAAAGTCGA ATGGGGATTT GCTGGCTCCT CCAGCTAAAC AACAAAAGAC CATATTTCCT 
GCCTACTTCA TTCCCGAGCT GGCTCATGTG GTGGCCATGT GCGATGAAAA GATACCGTTC 
ATGAACCTAG CCCAGACGCT GTCCAAGCAC AATATTCCCC ACAGCGGACT GCAGGTGGAA 
GCGAATGCCA CGTCACTTGT TCTGAAGATA CTGGCCTTAC CACAGCCAGG AAAAACAACC 
TTTGCAACCC AACAACAGCA GCAACAGGGA GCACCTGCAG TGGCAGGAGA AAATAAGCCA 
AGTGGTACCT CTGGACTGCC GAAAATCGAC TCCCACGTGT GGGACGACCT AATGCGTCGC 
GTTCTCTCCA TATCGATTCG TTCACAGACC AACAAGAACA GCCAGGTCCG GATCTGGGTA 
GTGGAGTTCG TCTTCTACAG CACACCGCTG CAAAGTAGCC ATCCGAAGGA GCAGGGCAGT 
CGGCGGACTG TTTACCTCAC CTACGAACAG GCGAACTACG ACTTTTCAAA AACAGTTGAG 
GATTTGCTAA ACGACTGGTC GAAAATCGTG TACCTTTACA CGCTGGTTTA CGACTTTGCT 
GAGCAGCTGC TAAATAAACG TCTTTCCCTT TGTGACATGC TAGTGGTCAA GTCATACAGC 
TACATGAACC TGCTCCTCGG ATACGGACCC AAAAAAGAGG TGAGCTGCAA TATCTATTGG 
TCGGTGCAAT CCCACGGCTT CCGACTGACG TTCGTCGGCG GAATGTCAGC TGTAAACGGG 
CACTCAATGA TGCGTGACCA GCTGGCGCAA CACTTGAACC AGCAGCATAG CATCACCCAA 
ATCGCCCAGA TTCTCCATGA AACCTACAAC CCGTTGAGCT CCATTGCCAA GCTGCCGGTG 
CTGCCCTTCT TGGGGATACC GCGCCCTCAG GTGCCAGTGC TATCGTTTTG CGTGTTGGCA 
CAATCGCCGT GCCTGATAAG GTTGACATAT CAGGCAGTGT ACTGCCTGGA GTTGCGTTTC 
CGTGCCAACA GACTCGTATC CATCCGAGAT GGAGCGAGCA GTCGCTTCGA GCGTAACGTC 
GTGGAAGAGT TCACGCCCAT TCAGGGTCTG AAAGCCTTCC TCTCCAAGTA CGTGGACGAG 
TCGGCGGTCT ACAGGGGACG GGCCTCCCAC GAGGACGACA ATCCGCTGTC ACCAATGGGA 
ATGGAGGACA ACTTCGGTGG ACCGAGCAGT GTTGCGGGGG TAAGTGCTGG TGGATCCTCA 
CCATTTTTGG GTACTGGAAT GCGGGGACCG CAGTCGCCGA GAGACAGTGG CCTGCGTTTT 
CCTGCCCCCC ACACTCCGCC CTCCAGTTCA AATCCGCACA CACCCGCCAG TCCGCATCCA 
AGTGCGGGGG CAGGTGGCGG CAGTGGACCC CAGGGACACG GCAACTTCAA CCTGACGTCA 
CCGCCAGCGC CACATATGCC ACATCCGTCG CCAAGCGGCT TGATGCCGTC GTCTCCGCTA 
AATCCTCAGC CCAGTCCCCA CATGGTCCAT AGTCCTGGAC CCAACACGCT CTACATGCAG 
AGCCACCAGG ACTCTCCCTT CACCGCCATG TCGCCGGCGA ATAACAATTG GCCCGGATCC 
CCCAGCATGC CGCGCCCATC TCCACGACCC GGACAGAGTC CTGATCACAA GAGCACCGGT 
GGTGGAGCAG GAGTGGCCGG TGGAACGGAT AGAGGAGGCT CTCGGGGCAC TCTCAATCGG 
CCCTGGGCTG GAGCAGTACC TACTCTCCTC ACCCATGAAG CTTTGGAGAC TCTGTGTCGT 
CCCAGTCCGT ATCCGAACAA GGATATCAAT GTGCCGGACA TGAGTCCTCT AGAGCGTTTT 
CTAGGCTGCG TTTATATGAG AAGGCAATTG CACAGAAACA TTCAGAGCGA GGAGACGCTT 
ACCGCCCTAA ACAGTACAGA ACCAGGAGTG GTGCTCTTCA AGGTCGATGG ACTGCAGTGC 
CAGGTGGTGC TGAATCAGAT GCACATGCAG ACGCTGCACC TCAAAGTCTC TCAGCTGCCG 
CCCATGCCCG ACAAGCATCC GCCGCCGTTC CAGCTTAGCC AGGACGATCT GCTGGTTATA 
GAGCAGTACT TTGACACCAG AGTGGCAGCT CCACCATACC GACCCAATTC CCTGCACAGC 
ATCTGCCGGC TGTTGAACCT GCCCGCCCAG GTACTTAAGG ATTTCGTACA GATCATGCGT 
TTGGATTTGA AGCCGGAGCT GGGTGGCGAC CAGTTGAAAT GGACGGTGCA GATATGTTTG 
CGCATGCCGC CCTCGGCGGT TCCCATTGTG CCCAGTGGCA ATGCATGCGT GGTTATGGGT 
CGCATGAAGA TACTGTTCTT CCTGCAAATC ACGAGGATAC CCTACGGCGC GGTCATAGGC 
GTGGGCAAGG ACTGGAAGGA CAGCCCCTCT TTGGTGTTGC CCATTGTGTA CGACATTCAG 
ACGAACGTCA CCCAGCTGGC GGAACGCACA GGACAAGTGA TCTCGCCCAC AATGACCGCT 
GCTAGCACTC TTCTTCGACG CTTTGCCGAG TTCAATGCGC AACAGAATCA GTGTACGCTC 
TTCCCAGCCA TTACGGATCT TCTGACGAAC CTCCAGCTGG CTGCCGAGAT GCCACAGCCC 
CCACCCAACC AATCCATAGG ACCTCCCGTA GGCGTGGGTG TCGGAGTGGG ATCCAGTCCG 
AATCCGATGA TGCCCATGCA GCAGCTGCCG CAGCAAGTGG GACCGCAGGG CCCAGTGGGT 
CCCGGCGGCT ACCCGCAGAT GGGCCCCAAT CCCGGTGGCC CGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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MED14 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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