Nplp1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nplp1
Entrez Gene ID	38003
Full Name	Neuropeptide-like precursor 1
Synonyms	APK,CG3441,Dmel\CG3441,IPNa,IPNamide,MTY-amide,MTYa,MTYamide,NPLP-1,NPLP1,VQQ,nplp1
General protein information	Preferred Names Neuropeptide-like precursor 1 Names CG3441 gene product from transcript CG3441-RC APK peptide CG3441-PA CG3441-PB CG3441-PC IPNamide IPNamide peptide MTYamide peptide Nplp1-PA Nplp1-PB Nplp1-PC neuropeptide like precursor 1 neuropeptide like precursor protein 1 neuropeptide-like precursor 1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 60F1-60F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_138149.3	NP_611993.1	Neuropeptide-like precursor 1, isoform A
NM_001043105.3	NP_001036570.1	Neuropeptide-like precursor 1, isoform B
NM_001144288.2	NP_001137760.1	Neuropeptide-like precursor 1, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Nplp1 neuropeptide neurons in the developing Drosophila nerve cord belong to two sub-classes; Tv1 and dAp neurons, generated by two distinct progenitors. Nplp1 neurons are specified by spatial cues
Title: Neuronal cell fate specification by the molecular convergence of different spatio-temporal cues on a common initiator terminal selector gene.

The following Nplp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nplp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa11283	NM_138149.3 Latest version!	Drosophila melanogaster Neuropeptide-like precursor 1 (Nplp1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa11284	NM_001043105.3 Latest version!	Drosophila melanogaster Neuropeptide-like precursor 1 (Nplp1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa13246	NM_001144288.2 Latest version!	Drosophila melanogaster Neuropeptide-like precursor 1 (Nplp1), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nplp1 ( NM_138149.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611993.1 Drosophila melanogaster Neuropeptide-like precursor 1 (Nplp1), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa11283
Clone ID Related Accession (Same CDS sequence)	NM_138149.3
Accession Version	NM_138149.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 930bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Neuropeptide-like precursor 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_138149.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGGCGG TGCTCCAATC TGCGCACAGC AGCAGGCGGC TAATGCTGTT GCTCTCCATG 
CTGCTCAATG CCGCTATTCA GCCACGGTCC ATTATTGTGA GTGCTACTGA TGATGTCGCC 
AATGTGTCGC CGTGCGAAAT GGAGTCCCTT ATAAATCAAC TGATGAGCCC CAGTCCCGAG 
TACCAGCTTC ACGCCTCCGC ATTGCGCAAT CAACTGAAAA ACTTACTTCG GGAAAGACAG 
TTGGCCGTGG GCGAAGAACA GCCTTTGGGC GAGTACCCCG ATTATCTGGA GGAGGACAAA 
CGTTCGGTGG CCGCACTGGC CGCCCAAGGC CTCCTTAACG CTCCGAAGAG AAGCTTGGCC 
ACTCTGGCTA AAAACGGGCA ACTGCCCACG GCAGAGCCTG GAGAGGATTA CGGCGACGCG 
GACTCCGGAG AACCGAGTGA ACAGAAGCGC TACATCGGCT CCTTGGCCAG GGCCGGCGGA 
TTGATGACAT ATGGAAAGCG CAACGTGGGC ACCCTGGCCC GCGACTTCCA GCTGCCTATT 
CCCAACGGCA AGCGAAACAT CGCAACTATG GCCCGTCTGC AGAGTGCTCC CTCCACCCAC 
CGAGATCCAA AGCGCAATGT GGCCGCCGTC GCACGCTACA ACTCGCAGCA CGGTCACATC 
CAGCGTGCTG GGGCCGAGAA ACGTAACCTG GGAGCTCTGA AGTCCTCGCC GGTCCACGGA 
GTGCAGCAGA AGCGCGAGGA TGAAGAGATG CTGTTGCCCG CGGCCGCCCC CGACTACGCC 
GATCCCATGC AGAGCTACTG GTGGTACCCC AGCTACGCCG GGTACGCCGA CCTCGACTGG 
AACGATTATC GCCGGGCAGA GAAGCGATTC CTAGGCCGGG TACTTCCACC AACACGAGCG 
ACTGCGTCAA CCCACCGCAG CCGTTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611993.1
CDS	232..1161
Translation	MQAVLQSAHSSRRLMLLLSMLLNAAIQPRSIIVSATDDVANVSPCEMESLINQLMSPSPEYQLHASALRNQLKNLLRERQLAVGEEQPLGEYPDYLEEDKRSVAALAAQGLLNAPKRSLATLAKNGQLPTAEPGEDYGDADSGEPSEQKRYIGSLARAGGLMTYGKRNVGTLARDFQLPIPNGKRNIATMARLQSAPSTHRDPKRNVAAVARYNSQHGHIQRAGAEKRNLGALKSSPVHGVQQKREDEEMLLPAAAPDYADPMQSYWWYPSYAGYADLDWNDYRRAEKRFLGRVLPPTRATASTHRSRL

Target ORF information:

RefSeq Version	NM_138149.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Neuropeptide-like precursor 1 (Nplp1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138149.3

ORF Insert Sequence:

ATGCAGGCGG TGCTCCAATC TGCGCACAGC AGCAGGCGGC TAATGCTGTT GCTCTCCATG 
CTGCTCAATG CCGCTATTCA GCCACGGTCC ATTATTGTGA GTGCTACTGA TGATGTCGCC 
AATGTGTCGC CGTGCGAAAT GGAGTCCCTT ATAAATCAAC TGATGAGCCC CAGTCCCGAG 
TACCAGCTTC ACGCCTCCGC ATTGCGCAAT CAACTGAAAA ACTTACTTCG GGAAAGACAG 
TTGGCCGTGG GCGAAGAACA GCCTTTGGGC GAGTACCCCG ATTATCTGGA GGAGGACAAA 
CGTTCGGTGG CCGCACTGGC CGCCCAAGGC CTCCTTAACG CTCCGAAGAG AAGCTTGGCC 
ACTCTGGCTA AAAACGGGCA ACTGCCCACG GCAGAGCCTG GAGAGGATTA CGGCGACGCG 
GACTCCGGAG AACCGAGTGA ACAGAAGCGC TACATCGGCT CCTTGGCCAG GGCCGGCGGA 
TTGATGACAT ATGGAAAGCG CAACGTGGGC ACCCTGGCCC GCGACTTCCA GCTGCCTATT 
CCCAACGGCA AGCGAAACAT CGCAACTATG GCCCGTCTGC AGAGTGCTCC CTCCACCCAC 
CGAGATCCAA AGCGCAATGT GGCCGCCGTC GCACGCTACA ACTCGCAGCA CGGTCACATC 
CAGCGTGCTG GGGCCGAGAA ACGTAACCTG GGAGCTCTGA AGTCCTCGCC GGTCCACGGA 
GTGCAGCAGA AGCGCGAGGA TGAAGAGATG CTGTTGCCCG CGGCCGCCCC CGACTACGCC 
GATCCCATGC AGAGCTACTG GTGGTACCCC AGCTACGCCG GGTACGCCGA CCTCGACTGG 
AACGATTATC GCCGGGCAGA GAAGCGATTC CTAGGCCGGG TACTTCCACC AACACGAGCG 
ACTGCGTCAA CCCACCGCAG CCGTTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nplp1 ( NM_001043105.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001036570.1 Drosophila melanogaster Neuropeptide-like precursor 1 (Nplp1), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa11284
Clone ID Related Accession (Same CDS sequence)	NM_001043105.3
Accession Version	NM_001043105.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Neuropeptide-like precursor 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001043105.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGGCGG TGCTCCAATC TGCGCACAGC AGCAGGCGGC TAATGCTGTT GCTCTCCATG 
CTGCTCAATG CCGCTATTCA GCCACGGTCC ATTATTGTGA GTGCTACTGA TGATGTCGCC 
AATGTGTCGC CGTGCGAAAT GGAGTCCCTT ATAAATCAAC TGATGAGCCC CAGTCCCGAG 
TACCAGCTTC ACGCCTCCGC ATTGCGCAAT CAACTGAAAA ACTTACTTCG GGAAAGACAG 
TTGGCCGTGG GCGAAGAACA GCCTTTGGGC GAGTACCCCG ATTATCTGGA GGAGGACAAA 
CGTTCGGTGG CCGCACTGGC CGCCCAAGGC CTCCTTAACG CTCCGAAGAG AAGCTTGGCC 
ACTCTGGCTA AAAACGGGCA ACTGCCCACG GCAGAGCCTG GAGAGGATTA CGGCGACGCG 
GACTCCGGAG AACCGAGTGA ACAGAAGCGC TACATCGGCT CCTTGGCCAG GGCCGGCGGA 
TTGATGACAT ATGGAAAGCG CAACGTGGGC ACCCTGGCCC GCGACTTCCA GCTGCCTATT 
CCCAACGGCA AGCGAAACAT CGCAACTATG GCCCGTCTGC AGAGTGCTCC CTCCACCCAC 
CGAGATCCAA AGCGCAATGT GGCCGCCGTC GCACGCTACA ACTCGCAGCA CGGTCACATC 
CAGCGTGCTG GGGCCGAGAA ACGTAACCTG GGAGCTCTGA AGTCCTCGCC GGTCCACGGA 
GTGCAGCAGA AGCGCGAGGA TGAAGAGATG CTGTTGCCCG CGGCCGCCCC CGACTACGCC 
GATCCCATGC AGAGCTACTG GTGGTACCCC AGCTACGCCG GGTACGCCGA CCTCGACTGG 
AACGATTATC GCCGGGCAGA GAAGCGATTC CTAGACACAT CCAAGGATCC CGAATTGTTC 
GGCATCGAGC ATGGCAACGA TGCCACTACT GCAGAACCCG CAGACGAGGC GTATATGGAG 
TCGGATGCGG AGGCCGGGAG CGAGCAGTTG CCATCGCCGC AGAAGCGTCA CATAGGCGCC 
GTTTATCGCT CCGGATTCCT GCCCAGTTAC CGCTACCTGC GCAGCCCTGG GGGCAGTAGT 
GGTTTTGGCG GGGCCGGGGG GCGTTTTAGT CGCTCGGGAC GTGACGCCAG GCAATTTGTA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036570.1
CDS	232..1374
Translation	MQAVLQSAHSSRRLMLLLSMLLNAAIQPRSIIVSATDDVANVSPCEMESLINQLMSPSPEYQLHASALRNQLKNLLRERQLAVGEEQPLGEYPDYLEEDKRSVAALAAQGLLNAPKRSLATLAKNGQLPTAEPGEDYGDADSGEPSEQKRYIGSLARAGGLMTYGKRNVGTLARDFQLPIPNGKRNIATMARLQSAPSTHRDPKRNVAAVARYNSQHGHIQRAGAEKRNLGALKSSPVHGVQQKREDEEMLLPAAAPDYADPMQSYWWYPSYAGYADLDWNDYRRAEKRFLDTSKDPELFGIEHGNDATTAEPADEAYMESDAEAGSEQLPSPQKRHIGAVYRSGFLPSYRYLRSPGGSSGFGGAGGRFSRSGRDARQFV

Target ORF information:

RefSeq Version	NM_001043105.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Neuropeptide-like precursor 1 (Nplp1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001043105.3

ORF Insert Sequence:

ATGCAGGCGG TGCTCCAATC TGCGCACAGC AGCAGGCGGC TAATGCTGTT GCTCTCCATG 
CTGCTCAATG CCGCTATTCA GCCACGGTCC ATTATTGTGA GTGCTACTGA TGATGTCGCC 
AATGTGTCGC CGTGCGAAAT GGAGTCCCTT ATAAATCAAC TGATGAGCCC CAGTCCCGAG 
TACCAGCTTC ACGCCTCCGC ATTGCGCAAT CAACTGAAAA ACTTACTTCG GGAAAGACAG 
TTGGCCGTGG GCGAAGAACA GCCTTTGGGC GAGTACCCCG ATTATCTGGA GGAGGACAAA 
CGTTCGGTGG CCGCACTGGC CGCCCAAGGC CTCCTTAACG CTCCGAAGAG AAGCTTGGCC 
ACTCTGGCTA AAAACGGGCA ACTGCCCACG GCAGAGCCTG GAGAGGATTA CGGCGACGCG 
GACTCCGGAG AACCGAGTGA ACAGAAGCGC TACATCGGCT CCTTGGCCAG GGCCGGCGGA 
TTGATGACAT ATGGAAAGCG CAACGTGGGC ACCCTGGCCC GCGACTTCCA GCTGCCTATT 
CCCAACGGCA AGCGAAACAT CGCAACTATG GCCCGTCTGC AGAGTGCTCC CTCCACCCAC 
CGAGATCCAA AGCGCAATGT GGCCGCCGTC GCACGCTACA ACTCGCAGCA CGGTCACATC 
CAGCGTGCTG GGGCCGAGAA ACGTAACCTG GGAGCTCTGA AGTCCTCGCC GGTCCACGGA 
GTGCAGCAGA AGCGCGAGGA TGAAGAGATG CTGTTGCCCG CGGCCGCCCC CGACTACGCC 
GATCCCATGC AGAGCTACTG GTGGTACCCC AGCTACGCCG GGTACGCCGA CCTCGACTGG 
AACGATTATC GCCGGGCAGA GAAGCGATTC CTAGACACAT CCAAGGATCC CGAATTGTTC 
GGCATCGAGC ATGGCAACGA TGCCACTACT GCAGAACCCG CAGACGAGGC GTATATGGAG 
TCGGATGCGG AGGCCGGGAG CGAGCAGTTG CCATCGCCGC AGAAGCGTCA CATAGGCGCC 
GTTTATCGCT CCGGATTCCT GCCCAGTTAC CGCTACCTGC GCAGCCCTGG GGGCAGTAGT 
GGTTTTGGCG GGGCCGGGGG GCGTTTTAGT CGCTCGGGAC GTGACGCCAG GCAATTTGTA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nplp1 ( NM_001144288.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137760.1 Drosophila melanogaster Neuropeptide-like precursor 1 (Nplp1), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa13246
Clone ID Related Accession (Same CDS sequence)	NM_001144288.2
Accession Version	NM_001144288.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1464bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Neuropeptide-like precursor 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001144288.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGGCGG TGCTCCAATC TGCGCACAGC AGCAGGCGGC TAATGCTGTT GCTCTCCATG 
CTGCTCAATG CCGCTATTCA GCCACGGTCC ATTATTGTGA GTGCTACTGA TGATGTCGCC 
AATGTGTCGC CGTGCGAAAT GGAGTCCCTT ATAAATCAAC TGATGAGCCC CAGTCCCGAG 
TACCAGCTTC ACGCCTCCGC ATTGCGCAAT CAACTGAAAA ACTTACTTCG GGAAAGACAG 
TTGGCCGTGG GCGAAGAACA GCCTTTGGGC GAGTACCCCG ATTATCTGGA GGAGGACAAA 
CGTTCGGTGG CCGCACTGGC CGCCCAAGGC CTCCTTAACG CTCCGAAGAG AAGCTTGGCC 
ACTCTGGCTA AAAACGGGCA ACTGCCCACG GCAGAGCCTG GAGAGGATTA CGGCGACGCG 
GACTCCGGAG AACCGAGTGA ACAGAAGCGC TACATCGGCT CCTTGGCCAG GGCCGGCGGA 
TTGATGACAT ATGGAAAGCG CAACGTGGGC ACCCTGGCCC GCGACTTCCA GCTGCCTATT 
CCCAACGGCA AGCGAAACAT CGCAACTATG GCCCGTCTGC AGAGTGCTCC CTCCACCCAC 
CGAGATCCAA AGCGCAATGT GGCCGCCGTC GCACGCTACA ACTCGCAGCA CGGTCACATC 
CAGCGTGCTG GGGCCGAGAA ACGTAACCTG GGAGCTCTGA AGTCCTCGCC GGTCCACGGA 
GTGCAGCAGA AGCGCGAGGA TGAAGAGATG CTGTTGCCCG CGGCCGCCCC CGACTACGCC 
GATCCCATGC AGAGCTACTG GTGGTACCCC AGCTACGCCG GGTACGCCGA CCTCGACTGG 
AACGATTATC GCCGGGCAGA GAAGCGATTC CTAGACACAT CCAAGGATCC CGAATTGTTC 
GGCATCGAGC ATGGCAACGA TGCCACTACT GCAGAACCCG CAGACGAGGC GTATATGGAG 
TCGGATGCGG AGGCCGGGAG CGAGCAGTTG CCATCGCCGC AGAAGCGTCA CATAGGCGCC 
GTTTATCGCT CCGGATTCCT GCCCAGTTAC CGCTACCTGC GCAGCCCTGG GGGCAGTAGT 
GGTTTTGGCG GGGCCGGGGG GCGTTTTAGT CGCTCGGGAC GTGACGCCAG GCAATTTGCC 
GGGTACTTCC ACCAACACGA GCGACTGCGT CAACCCACCG CAGCCGTTTG TAAGCAGTGT 
TTCATACCTA ACCAACCGAT GATCAATTGG AGTGGTGCTG GCGTACGCGG TCGTCTGTCC 
AATTACTATG TGGATCCCGA GTCCTCATTA GCGCGCTTGC CGAGCCTGTC AAGTAACTCG 
CTGCCATCAG GACGCCCTCC GCGCCCGCTC CTGCGCTCAG GGGCTCCAGT CTATCCGCCC 
TTCCACACCT GGGGCACTCC GCCGCGTATC ACAGCACTGC ATCGACGAGA ATTTCGACGC 
AACTCGCAAA ATTACGAATA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137760.1
CDS	232..1695
Translation	MQAVLQSAHSSRRLMLLLSMLLNAAIQPRSIIVSATDDVANVSPCEMESLINQLMSPSPEYQLHASALRNQLKNLLRERQLAVGEEQPLGEYPDYLEEDKRSVAALAAQGLLNAPKRSLATLAKNGQLPTAEPGEDYGDADSGEPSEQKRYIGSLARAGGLMTYGKRNVGTLARDFQLPIPNGKRNIATMARLQSAPSTHRDPKRNVAAVARYNSQHGHIQRAGAEKRNLGALKSSPVHGVQQKREDEEMLLPAAAPDYADPMQSYWWYPSYAGYADLDWNDYRRAEKRFLDTSKDPELFGIEHGNDATTAEPADEAYMESDAEAGSEQLPSPQKRHIGAVYRSGFLPSYRYLRSPGGSSGFGGAGGRFSRSGRDARQFAGYFHQHERLRQPTAAVCKQCFIPNQPMINWSGAGVRGRLSNYYVDPESSLARLPSLSSNSLPSGRPPRPLLRSGAPVYPPFHTWGTPPRITALHRREFRRNSQNYEY

Target ORF information:

RefSeq Version	NM_001144288.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Neuropeptide-like precursor 1 (Nplp1), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144288.2

ORF Insert Sequence:

ATGCAGGCGG TGCTCCAATC TGCGCACAGC AGCAGGCGGC TAATGCTGTT GCTCTCCATG 
CTGCTCAATG CCGCTATTCA GCCACGGTCC ATTATTGTGA GTGCTACTGA TGATGTCGCC 
AATGTGTCGC CGTGCGAAAT GGAGTCCCTT ATAAATCAAC TGATGAGCCC CAGTCCCGAG 
TACCAGCTTC ACGCCTCCGC ATTGCGCAAT CAACTGAAAA ACTTACTTCG GGAAAGACAG 
TTGGCCGTGG GCGAAGAACA GCCTTTGGGC GAGTACCCCG ATTATCTGGA GGAGGACAAA 
CGTTCGGTGG CCGCACTGGC CGCCCAAGGC CTCCTTAACG CTCCGAAGAG AAGCTTGGCC 
ACTCTGGCTA AAAACGGGCA ACTGCCCACG GCAGAGCCTG GAGAGGATTA CGGCGACGCG 
GACTCCGGAG AACCGAGTGA ACAGAAGCGC TACATCGGCT CCTTGGCCAG GGCCGGCGGA 
TTGATGACAT ATGGAAAGCG CAACGTGGGC ACCCTGGCCC GCGACTTCCA GCTGCCTATT 
CCCAACGGCA AGCGAAACAT CGCAACTATG GCCCGTCTGC AGAGTGCTCC CTCCACCCAC 
CGAGATCCAA AGCGCAATGT GGCCGCCGTC GCACGCTACA ACTCGCAGCA CGGTCACATC 
CAGCGTGCTG GGGCCGAGAA ACGTAACCTG GGAGCTCTGA AGTCCTCGCC GGTCCACGGA 
GTGCAGCAGA AGCGCGAGGA TGAAGAGATG CTGTTGCCCG CGGCCGCCCC CGACTACGCC 
GATCCCATGC AGAGCTACTG GTGGTACCCC AGCTACGCCG GGTACGCCGA CCTCGACTGG 
AACGATTATC GCCGGGCAGA GAAGCGATTC CTAGACACAT CCAAGGATCC CGAATTGTTC 
GGCATCGAGC ATGGCAACGA TGCCACTACT GCAGAACCCG CAGACGAGGC GTATATGGAG 
TCGGATGCGG AGGCCGGGAG CGAGCAGTTG CCATCGCCGC AGAAGCGTCA CATAGGCGCC 
GTTTATCGCT CCGGATTCCT GCCCAGTTAC CGCTACCTGC GCAGCCCTGG GGGCAGTAGT 
GGTTTTGGCG GGGCCGGGGG GCGTTTTAGT CGCTCGGGAC GTGACGCCAG GCAATTTGCC 
GGGTACTTCC ACCAACACGA GCGACTGCGT CAACCCACCG CAGCCGTTTG TAAGCAGTGT 
TTCATACCTA ACCAACCGAT GATCAATTGG AGTGGTGCTG GCGTACGCGG TCGTCTGTCC 
AATTACTATG TGGATCCCGA GTCCTCATTA GCGCGCTTGC CGAGCCTGTC AAGTAACTCG 
CTGCCATCAG GACGCCCTCC GCGCCCGCTC CTGCGCTCAG GGGCTCCAGT CTATCCGCCC 
TTCCACACCT GGGGCACTCC GCCGCGTATC ACAGCACTGC ATCGACGAGA ATTTCGACGC 
AACTCGCAAA ATTACGAATA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nplp1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

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