Pof cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pof
Entrez Gene ID	37947
Full Name	Painting of fourth
Synonyms	CG3691,Dmel\CG3691,POF,cg3691,pof
General protein information	Preferred Names Painting of fourth Names CG3691 gene product from transcript CG3691-RB CG3691-PA CG3691-PB CG3691-PC Pof-PA Pof-PB Pof-PC painting of fourth painting of fourth chromosome painting on the fourth painting-of-fourth
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 60D13-60D13

mRNA and Protein(s)

mRNA	Protein	Name
NM_079127.3	NP_523851.1	painting of fourth, isoform B
NM_001259569.2	NP_001246498.1	painting of fourth, isoform C
NM_166682.3	NP_726472.1	painting of fourth, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The targeting of protein Painting of fourth to PoX1(POF-on-X) and PoX2 is largely dependent on roX expression and identified a high-affinity target region that ectopically recruits POF.
Title: Targeting of Painting of fourth to roX1 and roX2 proximal sites suggests evolutionary links between dosage compensation and the regulation of the fourth chromosome in Drosophila melanogaster.

POF binds to nascent RNA, and this binding is associated with increased transcription output from chromosome 4
Title: POF regulates the expression of genes on the fourth chromosome in Drosophila melanogaster by binding to nascent RNA.

Together with Egg, Wde (Windei) localizes to euchromatin, is enriched on chromosome 4, and binds to the Painting of fourth (POF) protein.
Title: Windei, the Drosophila homolog of mAM/MCAF1, is an essential cofactor of the H3K9 methyl transferase dSETDB1/Eggless in germ line development.

These results suggest a balancing mechanism for the regulation of the fourth chromosome where POF and HP1 competitively bind at increasing levels with increased transcriptional activity.
Title: POF and HP1 bind expressed exons, suggesting a balancing mechanism for gene regulation.

There is a balancing mechanism involving POF and HP1 that provides a feedback system for fine-tuning expression status of genes on the 4th chromosome.
Title: Painting of fourth and chromosome-wide regulation of the 4th chromosome in Drosophila melanogaster.

The following Pof gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pof cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa11219	NM_001259569.2 Latest version!	Drosophila melanogaster painting of fourth, transcript variant C (Pof), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa11219	NM_166682.3 Latest version!	Drosophila melanogaster painting of fourth, transcript variant A (Pof), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa11219	NM_079127.3 Latest version!	Drosophila melanogaster painting of fourth, transcript variant B (Pof), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pof ( NM_079127.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523851.1 Drosophila melanogaster painting of fourth, transcript variant B (Pof), mRNA.

Price & Availability↑

CloneID	OFa11219
Clone ID Related Accession (Same CDS sequence)	NM_079127.3 , NM_001259569.2 , NM_166682.3
Accession Version	NM_079127.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1488bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	painting of fourth, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_079127.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATTCAA AACGCGCGGC CCTTGAATCC GGCGACGGTC CGGACGCCAA GCGCCTTGAT 
ACCACCGATG ATCAAGACAA AGAAGCGTCG GGGGGCGACG GCAGTCAGGT TATGCTGGCC 
AAACACGTAG CACCCTACAC GGGCCACGGA TGTACTCCAC CGATGGAGTC CTATTTGTTC 
GAACCGACTC CCGCCGGGAG TCAACTTCTG CCGTGGAAAA CTAGCGTTGA TTTGGACAAC 
GACGCCGAGC TGGAGAAGCC CACCAGTGAC AAGAAACCCG ACACTGCCAA GTTGTCCCGT 
CGGGAGCTGG CCAAAATGCG CCGTGAACAC ACATTAAGGG CCCTCGCCCT GGAACGCGAG 
TTGACCAACA AACCGGGGCA GACACCCGCT TCCGAAGTGT TGCTCGTCCG CTTTCCTGAT 
CCCGAGATTA CTGCTCCCAT GCTGGCAGGA CTGTCCAAGG ATATTCGGGA TGTGGTCTTG 
CCCATTAGCG TGGCTCCACG ATACTGTCTA GTGCATCTAA AGGCCGGTGC CGATGTGGAG 
GCCACCATCT GCGACATAAA CAGGGTGCGC TTCGGTACGG GGCACCTTCG GGCGGAGCTT 
AAGCCGTTCT CCGATGAGGA GCAGGCCGAG TTCATTGATC CCTGTTCTCT GTATGTGGGC 
AACATACCGT TCAACATGAC CACATCGGCC ATAAAGGCCT ATTTCGCCAA CGCCATGCGC 
GTGGACATCG GAGTGCTGAA GCGCGAGAAG CGCGCTCGCT ACGCCTTTGT GCGTTACGCC 
TCACCTGACC AGACCATGGA AGCGTTCAAG GAGCTGGTCG ACTCACCGCT GAACAGCCGC 
ACTCTCACAG TCCGCTATCG GCGACTCCGG AAGCGCGCCG GGATGCCAAT GGTGCAGTGC 
GCCACCTCCT TTCAGGCGCT GCAATCCCCA AATGGAGACG ACGACAACAC TGACTGCAAG 
GTCATATCTC CACCGCCGTT AGAGTCTATC ATAATTAGCG ACAGCGATAA CTGCTCAGAT 
TCCAGTGGCA ACGGCAAGGA AGACGGCAAG CGCAAGAAGA AGATAAATGA ACAGGAAAGA 
GAGATTGAAA AGCTCAAGCG TCAAATGGCC GAGTACGGCG CTATTATAAA AAGTTTGCAA 
TTTAGACAGA ACAGCTTAGA AGATACATTT ATACCTGACC TTACACCCAA AGTAGAGCCG 
AGCGTGAATC CCACAGGTTG TTTACTAGGA TCCAATGCGG TGCACCTAAT GCGTGACATT 
AAGAAAGAGT GCGACTATTT GGGCATCCCT GATCCCGTAC CGGCTACCAA GCCGACAACA 
CAAGCTCAAG ATGACAGCCA GAAGAAAGCC AAAAGATCGT GTTTTGGCCG CTTGTTTACG 
GGTCCTTTCA GACGAGGCAC TAGTGCGATG AAGACTGCTG ACGAGTACGA GAAGGATGAC 
AGACTTGAGG AGCTCTATGC TCAACTGGAA CGCGATCCTG ATCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523851.1
CDS	126..1613
Translation	MDSKRAALESGDGPDAKRLDTTDDQDKEASGGDGSQVMLAKHVAPYTGHGCTPPMESYLFEPTPAGSQLLPWKTSVDLDNDAELEKPTSDKKPDTAKLSRRELAKMRREHTLRALALERELTNKPGQTPASEVLLVRFPDPEITAPMLAGLSKDIRDVVLPISVAPRYCLVHLKAGADVEATICDINRVRFGTGHLRAELKPFSDEEQAEFIDPCSLYVGNIPFNMTTSAIKAYFANAMRVDIGVLKREKRARYAFVRYASPDQTMEAFKELVDSPLNSRTLTVRYRRLRKRAGMPMVQCATSFQALQSPNGDDDNTDCKVISPPPLESIIISDSDNCSDSSGNGKEDGKRKKKINEQEREIEKLKRQMAEYGAIIKSLQFRQNSLEDTFIPDLTPKVEPSVNPTGCLLGSNAVHLMRDIKKECDYLGIPDPVPATKPTTQAQDDSQKKAKRSCFGRLFTGPFRRGTSAMKTADEYEKDDRLEELYAQLERDPDP

Target ORF information:

RefSeq Version	NM_079127.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster painting of fourth, transcript variant B (Pof), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079127.3

ORF Insert Sequence:

ATGGATTCAA AACGCGCGGC CCTTGAATCC GGCGACGGTC CGGACGCCAA GCGCCTTGAT 
ACCACCGATG ATCAAGACAA AGAAGCGTCG GGGGGCGACG GCAGTCAGGT TATGCTGGCC 
AAACACGTAG CACCCTACAC GGGCCACGGA TGTACTCCAC CGATGGAGTC CTATTTGTTC 
GAACCGACTC CCGCCGGGAG TCAACTTCTG CCGTGGAAAA CTAGCGTTGA TTTGGACAAC 
GACGCCGAGC TGGAGAAGCC CACCAGTGAC AAGAAACCCG ACACTGCCAA GTTGTCCCGT 
CGGGAGCTGG CCAAAATGCG CCGTGAACAC ACATTAAGGG CCCTCGCCCT GGAACGCGAG 
TTGACCAACA AACCGGGGCA GACACCCGCT TCCGAAGTGT TGCTCGTCCG CTTTCCTGAT 
CCCGAGATTA CTGCTCCCAT GCTGGCAGGA CTGTCCAAGG ATATTCGGGA TGTGGTCTTG 
CCCATTAGCG TGGCTCCACG ATACTGTCTA GTGCATCTAA AGGCCGGTGC CGATGTGGAG 
GCCACCATCT GCGACATAAA CAGGGTGCGC TTCGGTACGG GGCACCTTCG GGCGGAGCTT 
AAGCCGTTCT CCGATGAGGA GCAGGCCGAG TTCATTGATC CCTGTTCTCT GTATGTGGGC 
AACATACCGT TCAACATGAC CACATCGGCC ATAAAGGCCT ATTTCGCCAA CGCCATGCGC 
GTGGACATCG GAGTGCTGAA GCGCGAGAAG CGCGCTCGCT ACGCCTTTGT GCGTTACGCC 
TCACCTGACC AGACCATGGA AGCGTTCAAG GAGCTGGTCG ACTCACCGCT GAACAGCCGC 
ACTCTCACAG TCCGCTATCG GCGACTCCGG AAGCGCGCCG GGATGCCAAT GGTGCAGTGC 
GCCACCTCCT TTCAGGCGCT GCAATCCCCA AATGGAGACG ACGACAACAC TGACTGCAAG 
GTCATATCTC CACCGCCGTT AGAGTCTATC ATAATTAGCG ACAGCGATAA CTGCTCAGAT 
TCCAGTGGCA ACGGCAAGGA AGACGGCAAG CGCAAGAAGA AGATAAATGA ACAGGAAAGA 
GAGATTGAAA AGCTCAAGCG TCAAATGGCC GAGTACGGCG CTATTATAAA AAGTTTGCAA 
TTTAGACAGA ACAGCTTAGA AGATACATTT ATACCTGACC TTACACCCAA AGTAGAGCCG 
AGCGTGAATC CCACAGGTTG TTTACTAGGA TCCAATGCGG TGCACCTAAT GCGTGACATT 
AAGAAAGAGT GCGACTATTT GGGCATCCCT GATCCCGTAC CGGCTACCAA GCCGACAACA 
CAAGCTCAAG ATGACAGCCA GAAGAAAGCC AAAAGATCGT GTTTTGGCCG CTTGTTTACG 
GGTCCTTTCA GACGAGGCAC TAGTGCGATG AAGACTGCTG ACGAGTACGA GAAGGATGAC 
AGACTTGAGG AGCTCTATGC TCAACTGGAA CGCGATCCTG ATCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pof ( NM_001259569.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246498.1 Drosophila melanogaster painting of fourth, transcript variant C (Pof), mRNA.

Price & Availability↑

CloneID	OFa11219
Clone ID Related Accession (Same CDS sequence)	NM_079127.3 , NM_001259569.2 , NM_166682.3
Accession Version	NM_001259569.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1488bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	painting of fourth, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001259569.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATTCAA AACGCGCGGC CCTTGAATCC GGCGACGGTC CGGACGCCAA GCGCCTTGAT 
ACCACCGATG ATCAAGACAA AGAAGCGTCG GGGGGCGACG GCAGTCAGGT TATGCTGGCC 
AAACACGTAG CACCCTACAC GGGCCACGGA TGTACTCCAC CGATGGAGTC CTATTTGTTC 
GAACCGACTC CCGCCGGGAG TCAACTTCTG CCGTGGAAAA CTAGCGTTGA TTTGGACAAC 
GACGCCGAGC TGGAGAAGCC CACCAGTGAC AAGAAACCCG ACACTGCCAA GTTGTCCCGT 
CGGGAGCTGG CCAAAATGCG CCGTGAACAC ACATTAAGGG CCCTCGCCCT GGAACGCGAG 
TTGACCAACA AACCGGGGCA GACACCCGCT TCCGAAGTGT TGCTCGTCCG CTTTCCTGAT 
CCCGAGATTA CTGCTCCCAT GCTGGCAGGA CTGTCCAAGG ATATTCGGGA TGTGGTCTTG 
CCCATTAGCG TGGCTCCACG ATACTGTCTA GTGCATCTAA AGGCCGGTGC CGATGTGGAG 
GCCACCATCT GCGACATAAA CAGGGTGCGC TTCGGTACGG GGCACCTTCG GGCGGAGCTT 
AAGCCGTTCT CCGATGAGGA GCAGGCCGAG TTCATTGATC CCTGTTCTCT GTATGTGGGC 
AACATACCGT TCAACATGAC CACATCGGCC ATAAAGGCCT ATTTCGCCAA CGCCATGCGC 
GTGGACATCG GAGTGCTGAA GCGCGAGAAG CGCGCTCGCT ACGCCTTTGT GCGTTACGCC 
TCACCTGACC AGACCATGGA AGCGTTCAAG GAGCTGGTCG ACTCACCGCT GAACAGCCGC 
ACTCTCACAG TCCGCTATCG GCGACTCCGG AAGCGCGCCG GGATGCCAAT GGTGCAGTGC 
GCCACCTCCT TTCAGGCGCT GCAATCCCCA AATGGAGACG ACGACAACAC TGACTGCAAG 
GTCATATCTC CACCGCCGTT AGAGTCTATC ATAATTAGCG ACAGCGATAA CTGCTCAGAT 
TCCAGTGGCA ACGGCAAGGA AGACGGCAAG CGCAAGAAGA AGATAAATGA ACAGGAAAGA 
GAGATTGAAA AGCTCAAGCG TCAAATGGCC GAGTACGGCG CTATTATAAA AAGTTTGCAA 
TTTAGACAGA ACAGCTTAGA AGATACATTT ATACCTGACC TTACACCCAA AGTAGAGCCG 
AGCGTGAATC CCACAGGTTG TTTACTAGGA TCCAATGCGG TGCACCTAAT GCGTGACATT 
AAGAAAGAGT GCGACTATTT GGGCATCCCT GATCCCGTAC CGGCTACCAA GCCGACAACA 
CAAGCTCAAG ATGACAGCCA GAAGAAAGCC AAAAGATCGT GTTTTGGCCG CTTGTTTACG 
GGTCCTTTCA GACGAGGCAC TAGTGCGATG AAGACTGCTG ACGAGTACGA GAAGGATGAC 
AGACTTGAGG AGCTCTATGC TCAACTGGAA CGCGATCCTG ATCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246498.1
CDS	122..1609
Translation	MDSKRAALESGDGPDAKRLDTTDDQDKEASGGDGSQVMLAKHVAPYTGHGCTPPMESYLFEPTPAGSQLLPWKTSVDLDNDAELEKPTSDKKPDTAKLSRRELAKMRREHTLRALALERELTNKPGQTPASEVLLVRFPDPEITAPMLAGLSKDIRDVVLPISVAPRYCLVHLKAGADVEATICDINRVRFGTGHLRAELKPFSDEEQAEFIDPCSLYVGNIPFNMTTSAIKAYFANAMRVDIGVLKREKRARYAFVRYASPDQTMEAFKELVDSPLNSRTLTVRYRRLRKRAGMPMVQCATSFQALQSPNGDDDNTDCKVISPPPLESIIISDSDNCSDSSGNGKEDGKRKKKINEQEREIEKLKRQMAEYGAIIKSLQFRQNSLEDTFIPDLTPKVEPSVNPTGCLLGSNAVHLMRDIKKECDYLGIPDPVPATKPTTQAQDDSQKKAKRSCFGRLFTGPFRRGTSAMKTADEYEKDDRLEELYAQLERDPDP

Target ORF information:

RefSeq Version	NM_001259569.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster painting of fourth, transcript variant C (Pof), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259569.2

ORF Insert Sequence:

ATGGATTCAA AACGCGCGGC CCTTGAATCC GGCGACGGTC CGGACGCCAA GCGCCTTGAT 
ACCACCGATG ATCAAGACAA AGAAGCGTCG GGGGGCGACG GCAGTCAGGT TATGCTGGCC 
AAACACGTAG CACCCTACAC GGGCCACGGA TGTACTCCAC CGATGGAGTC CTATTTGTTC 
GAACCGACTC CCGCCGGGAG TCAACTTCTG CCGTGGAAAA CTAGCGTTGA TTTGGACAAC 
GACGCCGAGC TGGAGAAGCC CACCAGTGAC AAGAAACCCG ACACTGCCAA GTTGTCCCGT 
CGGGAGCTGG CCAAAATGCG CCGTGAACAC ACATTAAGGG CCCTCGCCCT GGAACGCGAG 
TTGACCAACA AACCGGGGCA GACACCCGCT TCCGAAGTGT TGCTCGTCCG CTTTCCTGAT 
CCCGAGATTA CTGCTCCCAT GCTGGCAGGA CTGTCCAAGG ATATTCGGGA TGTGGTCTTG 
CCCATTAGCG TGGCTCCACG ATACTGTCTA GTGCATCTAA AGGCCGGTGC CGATGTGGAG 
GCCACCATCT GCGACATAAA CAGGGTGCGC TTCGGTACGG GGCACCTTCG GGCGGAGCTT 
AAGCCGTTCT CCGATGAGGA GCAGGCCGAG TTCATTGATC CCTGTTCTCT GTATGTGGGC 
AACATACCGT TCAACATGAC CACATCGGCC ATAAAGGCCT ATTTCGCCAA CGCCATGCGC 
GTGGACATCG GAGTGCTGAA GCGCGAGAAG CGCGCTCGCT ACGCCTTTGT GCGTTACGCC 
TCACCTGACC AGACCATGGA AGCGTTCAAG GAGCTGGTCG ACTCACCGCT GAACAGCCGC 
ACTCTCACAG TCCGCTATCG GCGACTCCGG AAGCGCGCCG GGATGCCAAT GGTGCAGTGC 
GCCACCTCCT TTCAGGCGCT GCAATCCCCA AATGGAGACG ACGACAACAC TGACTGCAAG 
GTCATATCTC CACCGCCGTT AGAGTCTATC ATAATTAGCG ACAGCGATAA CTGCTCAGAT 
TCCAGTGGCA ACGGCAAGGA AGACGGCAAG CGCAAGAAGA AGATAAATGA ACAGGAAAGA 
GAGATTGAAA AGCTCAAGCG TCAAATGGCC GAGTACGGCG CTATTATAAA AAGTTTGCAA 
TTTAGACAGA ACAGCTTAGA AGATACATTT ATACCTGACC TTACACCCAA AGTAGAGCCG 
AGCGTGAATC CCACAGGTTG TTTACTAGGA TCCAATGCGG TGCACCTAAT GCGTGACATT 
AAGAAAGAGT GCGACTATTT GGGCATCCCT GATCCCGTAC CGGCTACCAA GCCGACAACA 
CAAGCTCAAG ATGACAGCCA GAAGAAAGCC AAAAGATCGT GTTTTGGCCG CTTGTTTACG 
GGTCCTTTCA GACGAGGCAC TAGTGCGATG AAGACTGCTG ACGAGTACGA GAAGGATGAC 
AGACTTGAGG AGCTCTATGC TCAACTGGAA CGCGATCCTG ATCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pof ( NM_166682.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726472.1 Drosophila melanogaster painting of fourth, transcript variant A (Pof), mRNA.

Price & Availability↑

CloneID	OFa11219
Clone ID Related Accession (Same CDS sequence)	NM_079127.3 , NM_001259569.2 , NM_166682.3
Accession Version	NM_166682.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1488bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	painting of fourth, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166682.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATTCAA AACGCGCGGC CCTTGAATCC GGCGACGGTC CGGACGCCAA GCGCCTTGAT 
ACCACCGATG ATCAAGACAA AGAAGCGTCG GGGGGCGACG GCAGTCAGGT TATGCTGGCC 
AAACACGTAG CACCCTACAC GGGCCACGGA TGTACTCCAC CGATGGAGTC CTATTTGTTC 
GAACCGACTC CCGCCGGGAG TCAACTTCTG CCGTGGAAAA CTAGCGTTGA TTTGGACAAC 
GACGCCGAGC TGGAGAAGCC CACCAGTGAC AAGAAACCCG ACACTGCCAA GTTGTCCCGT 
CGGGAGCTGG CCAAAATGCG CCGTGAACAC ACATTAAGGG CCCTCGCCCT GGAACGCGAG 
TTGACCAACA AACCGGGGCA GACACCCGCT TCCGAAGTGT TGCTCGTCCG CTTTCCTGAT 
CCCGAGATTA CTGCTCCCAT GCTGGCAGGA CTGTCCAAGG ATATTCGGGA TGTGGTCTTG 
CCCATTAGCG TGGCTCCACG ATACTGTCTA GTGCATCTAA AGGCCGGTGC CGATGTGGAG 
GCCACCATCT GCGACATAAA CAGGGTGCGC TTCGGTACGG GGCACCTTCG GGCGGAGCTT 
AAGCCGTTCT CCGATGAGGA GCAGGCCGAG TTCATTGATC CCTGTTCTCT GTATGTGGGC 
AACATACCGT TCAACATGAC CACATCGGCC ATAAAGGCCT ATTTCGCCAA CGCCATGCGC 
GTGGACATCG GAGTGCTGAA GCGCGAGAAG CGCGCTCGCT ACGCCTTTGT GCGTTACGCC 
TCACCTGACC AGACCATGGA AGCGTTCAAG GAGCTGGTCG ACTCACCGCT GAACAGCCGC 
ACTCTCACAG TCCGCTATCG GCGACTCCGG AAGCGCGCCG GGATGCCAAT GGTGCAGTGC 
GCCACCTCCT TTCAGGCGCT GCAATCCCCA AATGGAGACG ACGACAACAC TGACTGCAAG 
GTCATATCTC CACCGCCGTT AGAGTCTATC ATAATTAGCG ACAGCGATAA CTGCTCAGAT 
TCCAGTGGCA ACGGCAAGGA AGACGGCAAG CGCAAGAAGA AGATAAATGA ACAGGAAAGA 
GAGATTGAAA AGCTCAAGCG TCAAATGGCC GAGTACGGCG CTATTATAAA AAGTTTGCAA 
TTTAGACAGA ACAGCTTAGA AGATACATTT ATACCTGACC TTACACCCAA AGTAGAGCCG 
AGCGTGAATC CCACAGGTTG TTTACTAGGA TCCAATGCGG TGCACCTAAT GCGTGACATT 
AAGAAAGAGT GCGACTATTT GGGCATCCCT GATCCCGTAC CGGCTACCAA GCCGACAACA 
CAAGCTCAAG ATGACAGCCA GAAGAAAGCC AAAAGATCGT GTTTTGGCCG CTTGTTTACG 
GGTCCTTTCA GACGAGGCAC TAGTGCGATG AAGACTGCTG ACGAGTACGA GAAGGATGAC 
AGACTTGAGG AGCTCTATGC TCAACTGGAA CGCGATCCTG ATCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726472.1
CDS	175..1662
Translation	MDSKRAALESGDGPDAKRLDTTDDQDKEASGGDGSQVMLAKHVAPYTGHGCTPPMESYLFEPTPAGSQLLPWKTSVDLDNDAELEKPTSDKKPDTAKLSRRELAKMRREHTLRALALERELTNKPGQTPASEVLLVRFPDPEITAPMLAGLSKDIRDVVLPISVAPRYCLVHLKAGADVEATICDINRVRFGTGHLRAELKPFSDEEQAEFIDPCSLYVGNIPFNMTTSAIKAYFANAMRVDIGVLKREKRARYAFVRYASPDQTMEAFKELVDSPLNSRTLTVRYRRLRKRAGMPMVQCATSFQALQSPNGDDDNTDCKVISPPPLESIIISDSDNCSDSSGNGKEDGKRKKKINEQEREIEKLKRQMAEYGAIIKSLQFRQNSLEDTFIPDLTPKVEPSVNPTGCLLGSNAVHLMRDIKKECDYLGIPDPVPATKPTTQAQDDSQKKAKRSCFGRLFTGPFRRGTSAMKTADEYEKDDRLEELYAQLERDPDP

Target ORF information:

RefSeq Version	NM_166682.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster painting of fourth, transcript variant A (Pof), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166682.3

ORF Insert Sequence:

ATGGATTCAA AACGCGCGGC CCTTGAATCC GGCGACGGTC CGGACGCCAA GCGCCTTGAT 
ACCACCGATG ATCAAGACAA AGAAGCGTCG GGGGGCGACG GCAGTCAGGT TATGCTGGCC 
AAACACGTAG CACCCTACAC GGGCCACGGA TGTACTCCAC CGATGGAGTC CTATTTGTTC 
GAACCGACTC CCGCCGGGAG TCAACTTCTG CCGTGGAAAA CTAGCGTTGA TTTGGACAAC 
GACGCCGAGC TGGAGAAGCC CACCAGTGAC AAGAAACCCG ACACTGCCAA GTTGTCCCGT 
CGGGAGCTGG CCAAAATGCG CCGTGAACAC ACATTAAGGG CCCTCGCCCT GGAACGCGAG 
TTGACCAACA AACCGGGGCA GACACCCGCT TCCGAAGTGT TGCTCGTCCG CTTTCCTGAT 
CCCGAGATTA CTGCTCCCAT GCTGGCAGGA CTGTCCAAGG ATATTCGGGA TGTGGTCTTG 
CCCATTAGCG TGGCTCCACG ATACTGTCTA GTGCATCTAA AGGCCGGTGC CGATGTGGAG 
GCCACCATCT GCGACATAAA CAGGGTGCGC TTCGGTACGG GGCACCTTCG GGCGGAGCTT 
AAGCCGTTCT CCGATGAGGA GCAGGCCGAG TTCATTGATC CCTGTTCTCT GTATGTGGGC 
AACATACCGT TCAACATGAC CACATCGGCC ATAAAGGCCT ATTTCGCCAA CGCCATGCGC 
GTGGACATCG GAGTGCTGAA GCGCGAGAAG CGCGCTCGCT ACGCCTTTGT GCGTTACGCC 
TCACCTGACC AGACCATGGA AGCGTTCAAG GAGCTGGTCG ACTCACCGCT GAACAGCCGC 
ACTCTCACAG TCCGCTATCG GCGACTCCGG AAGCGCGCCG GGATGCCAAT GGTGCAGTGC 
GCCACCTCCT TTCAGGCGCT GCAATCCCCA AATGGAGACG ACGACAACAC TGACTGCAAG 
GTCATATCTC CACCGCCGTT AGAGTCTATC ATAATTAGCG ACAGCGATAA CTGCTCAGAT 
TCCAGTGGCA ACGGCAAGGA AGACGGCAAG CGCAAGAAGA AGATAAATGA ACAGGAAAGA 
GAGATTGAAA AGCTCAAGCG TCAAATGGCC GAGTACGGCG CTATTATAAA AAGTTTGCAA 
TTTAGACAGA ACAGCTTAGA AGATACATTT ATACCTGACC TTACACCCAA AGTAGAGCCG 
AGCGTGAATC CCACAGGTTG TTTACTAGGA TCCAATGCGG TGCACCTAAT GCGTGACATT 
AAGAAAGAGT GCGACTATTT GGGCATCCCT GATCCCGTAC CGGCTACCAA GCCGACAACA 
CAAGCTCAAG ATGACAGCCA GAAGAAAGCC AAAAGATCGT GTTTTGGCCG CTTGTTTACG 
GGTCCTTTCA GACGAGGCAC TAGTGCGATG AAGACTGCTG ACGAGTACGA GAAGGATGAC 
AGACTTGAGG AGCTCTATGC TCAACTGGAA CGCGATCCTG ATCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Pof cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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