Fcp1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fcp1
Entrez Gene ID	37925
Full Name	TFIIF-interacting CTD phosphatase
Synonyms	21483550,CG12252,DmFcp1,Dmel\CG12252,Dmfcp1,fcp1
General protein information	Preferred Names TFIIF-interacting CTD phosphatase Names CG12252 gene product from transcript CG12252-RB CG12252-PA CG12252-PB Fcp1-PA Fcp1-PB RNApolymerase II carboxyl-terminal domain phosphatase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 60D5-60D5

mRNA and Protein(s)

mRNA	Protein	Name
NM_138090.2	NP_611934.1	TFIIF-interacting CTD phosphatase, isoform A
NM_001299917.1	NP_001286846.1	TFIIF-interacting CTD phosphatase, isoform B

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005119	XP_314000.3
Drosophila melanogaster (fruit fly)	Fcp1	NP_611934.1
Arabidopsis thaliana (thale cress)	CPL4	NP_001078764.1
Oryza sativa (rice)	Os05g0390500	NP_001055440.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

used transgene constructs to modify the Fcp1 phosphatase level in ovaries and found that high levels of Fcp1 are required for intensive gene expression in nurse cells
Title: High Fcp1 phosphatase activity contributes to setting an intense transcription rate required in Drosophila nurse and follicular cells for egg production.

Fcp1 binds to specific regions of the polytene chromosomes at many sites colocalized with Pol II.
Title: The RNA Pol II CTD phosphatase Fcp1 is essential for normal development in Drosophila melanogaster.

Results show that in Drosophila, misregulated expression of the RNA polymerase II carboxyl-terminal domain-specific phosphatase Fcp1 induces p53-dependent but transcription-independent apoptosis.
Title: Misregulated RNA Pol II C-terminal domain phosphorylation results in apoptosis.

The following Fcp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fcp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa11195	NM_138090.2 Latest version!	Drosophila melanogaster TFIIF-interacting CTD phosphatase (Fcp1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa11195	NM_001299917.1 Latest version!	Drosophila melanogaster TFIIF-interacting CTD phosphatase (Fcp1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fcp1 ( NM_138090.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611934.1 Drosophila melanogaster TFIIF-interacting CTD phosphatase (Fcp1), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa11195
Clone ID Related Accession (Same CDS sequence)	NM_138090.2 , NM_001299917.1
Accession Version	NM_138090.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2643bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	TFIIF-interacting CTD phosphatase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_138090.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGAACA TACCGGACGA GGAGGGCGCG ACGCCCAGCC GCGCGCCAGG GGGCGTGGCC 
GCATCTGGAG CTGCCGAAGG CGACGATGGC GGCGGAGGAA GCGGAGCCAA GAACAGCGCC 
AGCGGCAACA GCAACAACAA CACCAGCGGT GGCGGCATCA TTGTTTTGAG AGCTGCGCTG 
GGAGAGAACG AGGCGAGAGC GGTAATCAAT AAATGGCGGG TTCGCGAGGG ACAATTCGTG 
TCGGCGGCCC AAATCTTGTT TCTTTACCAG CCCGTGGGCG TGGACGCGAA GGACGCGAAG 
GACGCGGGAA AGCCGGGCGG GGACTGTGCC ATCCAGAGGT ACAAGTCCCA GCGCGCCGGA 
GTGGTGAAGA AGCGCCTTCG CAAGGAAGGG GAGCTTCTTA CGAAGGGGGA TGCCATTCTG 
GAGCTGTCCG AGTGCATACA CACCACGGTC ATCAAGGACA TGTGCGCGGA CTGCGGAGCC 
GACTTGCGTC AGAACGAGAA TGGACAGACC TCCGAGGCCT CGGTGCCAAT GGTGCACACC 
ATGCCCGATC TTAAGGTCAC CCAGAAGCTG GCCCAGAAGC TCGGGCACGA CGACACCCGC 
CGCCTGCTGG CGGATAGGAA GCTGGTCCTG CTTGTCGATC TCGATCAGAC GGTGATCCAC 
ACCACCAACG ACACTGTGCC GGACAACATC AAGGGCATCT ACCACTTCCA GCTGTACGGC 
CCACATTCCC CGTGGTACCA CACGCGCCTG CGCCCCGGCA CCGCCGAGTT TCTGGAGCGC 
ATGTCCCAGC TGTACGAACT GCACATTTGC ACTTTTGGTG CGCGTAACTA CGCGCACATG 
ATTGCCCAGC TGCTGGACCC GGAGGGCAAG TTCTTCTCGC ACAGGATTCT GTCCAGAGAC 
GAGTGTTTCA ACGCCACCAG CAAGACGGAC AATTTAAAGG CTCTGTTTCC GAATGGCGAT 
TCCATGGTGT GCATCATTGA CGATCGCGAG GATGTGTGGA ACATGGCTTC AAATTTGATT 
CAGGTTAAGC CCTACCACTT CTTTCAACAC ACTGGTGACA TCAATGCCCC GCCGGGTTTG 
TCTAAGCATG AACTGGATGG CGAAGGCGTT GACTTTAAAG AGATTACCGA GAAGCATGGG 
GACAAAGATA AGACGGAATC CAGCAGCGAA GTCAAACCAG AAGACACCGA TAAGGGTGAT 
AATACCGTCA CAAGCACAAG CAAAGATGAC GATGTAAACG AGGAGTCAGT AGACGTGTTC 
GAAATAGAGG GCGATGCCAA GGATCCAGAG GTTTCCAACG CCTCAAGCGC TACAGAAGCA 
CCCAAAGAGC CGAGAGACAA ATTGAATGGC AAGACTAATG CTGAGGATAT TGTGGTCATA 
GACGACAGCT CATCTGGCTC ACCAGATGCT GAAAAAGCAG CCAGCGATGG CGAAGACGTA 
GTAGTTATTG ATGATAATTC CAAGGAGAGC ACAAAAGCCG AGGTGCCCCC CACACCGGCA 
GAAAAGAATG AGGTTGTAGC CTCATCCACC ACTTCGCCGG ATGAGAAGCG CCCGTCTGCT 
GACGCTGATG TGGCCACCAC GTCTAAGACG CCCAGCTTAC GAGCACCGCT CGAGGGGCAA 
AAGCAGATAG AGATTGAAGA CCCCGACGAT TACCTGCTGT ATCTAGAGGT CATACTTCGT 
AACATTCACA AGCGATTCTA CTCTATTTAC GACGAAACCA CGGAAATACC CGATCTAAAG 
GTTATCGTTC CGAAGATTCG GAGCGAAGTG CTGCGCGGCA AGAACCTGGT GTTCTCCGGC 
CTGGTGCCCA CGCAGATGAA GCTCGAGCAG TCGCGGGCGT ATTTCATAGC CAAGAGCCTG 
GGCGCCGAAG TGAAGCCCAA TATTGACAAG GAGATAACGC ATCTAGTGGC GGTCAACGCA 
GGCACCTACA AGGTTAACGC CGCCAAAAAA GAGCCTGCCA TTAAAGTGGT CAATGCGAAT 
TGGTTGTGGA CCTGCGCCGA GCGCTGGGAG CACGTGGAGG AGAAGCTCTT TCCACTGGAC 
CGCAAGGTAC GCAACAAGGG TCGCCAGCCA CCCGCCCATT GCCATAGTCC CGAGCACGTG 
GTTAACTACA GCGAGCGTTC GGAGATCTCA CCCTCGAGCA GCAAGCAGCA GGAGGAGCAG 
AGCGGCAACT TCCGCGAAAC GCTGAATCCG CTGCTGGTCT TCACCAACGC AGATATCGAG 
TCTATGAATA AGGACTACGA AACATTCTTC GAGTCAGACT CGTCCAGCGA CGAGGGTCCT 
GTAAATTTTG AAAATCCACC AATGGACAAG AAGCTGTTGA AGAGAAAGCG CGAAGATGAT 
AATTCCAACA GAGCTCACGA CTTTTTCACG CGAAGCGATG ATATAATGAT TGGAGCGCCA 
AACCTCGTGG AGGTGGACAT TAGCTCGAAC GAGGAGGCCG ATGACAATAA CGAGAAGGAG 
GACGATGACG ATGAGATGCC CAGCGCCAAG TTTAGGCGAG GGGAAGACCT GCCTTCCGAC 
CTGGAGCTTG GATCCGAGTC CAACAGCGAG AAGGAACCGG AGGACGAGGA CGACGGCGAA 
TGGAACATGA TGGGCGCCGC TCTAGAAAGG GAGTTCCTCG GCCTGGAGGA CTTTGACATG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611934.1
CDS	137..2779
Translation	MQNIPDEEGATPSRAPGGVAASGAAEGDDGGGGSGAKNSASGNSNNNTSGGGIIVLRAALGENEARAVINKWRVREGQFVSAAQILFLYQPVGVDAKDAKDAGKPGGDCAIQRYKSQRAGVVKKRLRKEGELLTKGDAILELSECIHTTVIKDMCADCGADLRQNENGQTSEASVPMVHTMPDLKVTQKLAQKLGHDDTRRLLADRKLVLLVDLDQTVIHTTNDTVPDNIKGIYHFQLYGPHSPWYHTRLRPGTAEFLERMSQLYELHICTFGARNYAHMIAQLLDPEGKFFSHRILSRDECFNATSKTDNLKALFPNGDSMVCIIDDREDVWNMASNLIQVKPYHFFQHTGDINAPPGLSKHELDGEGVDFKEITEKHGDKDKTESSSEVKPEDTDKGDNTVTSTSKDDDVNEESVDVFEIEGDAKDPEVSNASSATEAPKEPRDKLNGKTNAEDIVVIDDSSSGSPDAEKAASDGEDVVVIDDNSKESTKAEVPPTPAEKNEVVASSTTSPDEKRPSADADVATTSKTPSLRAPLEGQKQIEIEDPDDYLLYLEVILRNIHKRFYSIYDETTEIPDLKVIVPKIRSEVLRGKNLVFSGLVPTQMKLEQSRAYFIAKSLGAEVKPNIDKEITHLVAVNAGTYKVNAAKKEPAIKVVNANWLWTCAERWEHVEEKLFPLDRKVRNKGRQPPAHCHSPEHVVNYSERSEISPSSSKQQEEQSGNFRETLNPLLVFTNADIESMNKDYETFFESDSSSDEGPVNFENPPMDKKLLKRKREDDNSNRAHDFFTRSDDIMIGAPNLVEVDISSNEEADDNNEKEDDDDEMPSAKFRRGEDLPSDLELGSESNSEKEPEDEDDGEWNMMGAALEREFLGLEDFDM

Target ORF information:

RefSeq Version	NM_138090.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster TFIIF-interacting CTD phosphatase (Fcp1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138090.2

ORF Insert Sequence:

ATGCAGAACA TACCGGACGA GGAGGGCGCG ACGCCCAGCC GCGCGCCAGG GGGCGTGGCC 
GCATCTGGAG CTGCCGAAGG CGACGATGGC GGCGGAGGAA GCGGAGCCAA GAACAGCGCC 
AGCGGCAACA GCAACAACAA CACCAGCGGT GGCGGCATCA TTGTTTTGAG AGCTGCGCTG 
GGAGAGAACG AGGCGAGAGC GGTAATCAAT AAATGGCGGG TTCGCGAGGG ACAATTCGTG 
TCGGCGGCCC AAATCTTGTT TCTTTACCAG CCCGTGGGCG TGGACGCGAA GGACGCGAAG 
GACGCGGGAA AGCCGGGCGG GGACTGTGCC ATCCAGAGGT ACAAGTCCCA GCGCGCCGGA 
GTGGTGAAGA AGCGCCTTCG CAAGGAAGGG GAGCTTCTTA CGAAGGGGGA TGCCATTCTG 
GAGCTGTCCG AGTGCATACA CACCACGGTC ATCAAGGACA TGTGCGCGGA CTGCGGAGCC 
GACTTGCGTC AGAACGAGAA TGGACAGACC TCCGAGGCCT CGGTGCCAAT GGTGCACACC 
ATGCCCGATC TTAAGGTCAC CCAGAAGCTG GCCCAGAAGC TCGGGCACGA CGACACCCGC 
CGCCTGCTGG CGGATAGGAA GCTGGTCCTG CTTGTCGATC TCGATCAGAC GGTGATCCAC 
ACCACCAACG ACACTGTGCC GGACAACATC AAGGGCATCT ACCACTTCCA GCTGTACGGC 
CCACATTCCC CGTGGTACCA CACGCGCCTG CGCCCCGGCA CCGCCGAGTT TCTGGAGCGC 
ATGTCCCAGC TGTACGAACT GCACATTTGC ACTTTTGGTG CGCGTAACTA CGCGCACATG 
ATTGCCCAGC TGCTGGACCC GGAGGGCAAG TTCTTCTCGC ACAGGATTCT GTCCAGAGAC 
GAGTGTTTCA ACGCCACCAG CAAGACGGAC AATTTAAAGG CTCTGTTTCC GAATGGCGAT 
TCCATGGTGT GCATCATTGA CGATCGCGAG GATGTGTGGA ACATGGCTTC AAATTTGATT 
CAGGTTAAGC CCTACCACTT CTTTCAACAC ACTGGTGACA TCAATGCCCC GCCGGGTTTG 
TCTAAGCATG AACTGGATGG CGAAGGCGTT GACTTTAAAG AGATTACCGA GAAGCATGGG 
GACAAAGATA AGACGGAATC CAGCAGCGAA GTCAAACCAG AAGACACCGA TAAGGGTGAT 
AATACCGTCA CAAGCACAAG CAAAGATGAC GATGTAAACG AGGAGTCAGT AGACGTGTTC 
GAAATAGAGG GCGATGCCAA GGATCCAGAG GTTTCCAACG CCTCAAGCGC TACAGAAGCA 
CCCAAAGAGC CGAGAGACAA ATTGAATGGC AAGACTAATG CTGAGGATAT TGTGGTCATA 
GACGACAGCT CATCTGGCTC ACCAGATGCT GAAAAAGCAG CCAGCGATGG CGAAGACGTA 
GTAGTTATTG ATGATAATTC CAAGGAGAGC ACAAAAGCCG AGGTGCCCCC CACACCGGCA 
GAAAAGAATG AGGTTGTAGC CTCATCCACC ACTTCGCCGG ATGAGAAGCG CCCGTCTGCT 
GACGCTGATG TGGCCACCAC GTCTAAGACG CCCAGCTTAC GAGCACCGCT CGAGGGGCAA 
AAGCAGATAG AGATTGAAGA CCCCGACGAT TACCTGCTGT ATCTAGAGGT CATACTTCGT 
AACATTCACA AGCGATTCTA CTCTATTTAC GACGAAACCA CGGAAATACC CGATCTAAAG 
GTTATCGTTC CGAAGATTCG GAGCGAAGTG CTGCGCGGCA AGAACCTGGT GTTCTCCGGC 
CTGGTGCCCA CGCAGATGAA GCTCGAGCAG TCGCGGGCGT ATTTCATAGC CAAGAGCCTG 
GGCGCCGAAG TGAAGCCCAA TATTGACAAG GAGATAACGC ATCTAGTGGC GGTCAACGCA 
GGCACCTACA AGGTTAACGC CGCCAAAAAA GAGCCTGCCA TTAAAGTGGT CAATGCGAAT 
TGGTTGTGGA CCTGCGCCGA GCGCTGGGAG CACGTGGAGG AGAAGCTCTT TCCACTGGAC 
CGCAAGGTAC GCAACAAGGG TCGCCAGCCA CCCGCCCATT GCCATAGTCC CGAGCACGTG 
GTTAACTACA GCGAGCGTTC GGAGATCTCA CCCTCGAGCA GCAAGCAGCA GGAGGAGCAG 
AGCGGCAACT TCCGCGAAAC GCTGAATCCG CTGCTGGTCT TCACCAACGC AGATATCGAG 
TCTATGAATA AGGACTACGA AACATTCTTC GAGTCAGACT CGTCCAGCGA CGAGGGTCCT 
GTAAATTTTG AAAATCCACC AATGGACAAG AAGCTGTTGA AGAGAAAGCG CGAAGATGAT 
AATTCCAACA GAGCTCACGA CTTTTTCACG CGAAGCGATG ATATAATGAT TGGAGCGCCA 
AACCTCGTGG AGGTGGACAT TAGCTCGAAC GAGGAGGCCG ATGACAATAA CGAGAAGGAG 
GACGATGACG ATGAGATGCC CAGCGCCAAG TTTAGGCGAG GGGAAGACCT GCCTTCCGAC 
CTGGAGCTTG GATCCGAGTC CAACAGCGAG AAGGAACCGG AGGACGAGGA CGACGGCGAA 
TGGAACATGA TGGGCGCCGC TCTAGAAAGG GAGTTCCTCG GCCTGGAGGA CTTTGACATG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fcp1 ( NM_001299917.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286846.1 Drosophila melanogaster TFIIF-interacting CTD phosphatase (Fcp1), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa11195
Clone ID Related Accession (Same CDS sequence)	NM_138090.2 , NM_001299917.1
Accession Version	NM_001299917.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2643bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	TFIIF-interacting CTD phosphatase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGAACA TACCGGACGA GGAGGGCGCG ACGCCCAGCC GCGCGCCAGG GGGCGTGGCC 
GCATCTGGAG CTGCCGAAGG CGACGATGGC GGCGGAGGAA GCGGAGCCAA GAACAGCGCC 
AGCGGCAACA GCAACAACAA CACCAGCGGT GGCGGCATCA TTGTTTTGAG AGCTGCGCTG 
GGAGAGAACG AGGCGAGAGC GGTAATCAAT AAATGGCGGG TTCGCGAGGG ACAATTCGTG 
TCGGCGGCCC AAATCTTGTT TCTTTACCAG CCCGTGGGCG TGGACGCGAA GGACGCGAAG 
GACGCGGGAA AGCCGGGCGG GGACTGTGCC ATCCAGAGGT ACAAGTCCCA GCGCGCCGGA 
GTGGTGAAGA AGCGCCTTCG CAAGGAAGGG GAGCTTCTTA CGAAGGGGGA TGCCATTCTG 
GAGCTGTCCG AGTGCATACA CACCACGGTC ATCAAGGACA TGTGCGCGGA CTGCGGAGCC 
GACTTGCGTC AGAACGAGAA TGGACAGACC TCCGAGGCCT CGGTGCCAAT GGTGCACACC 
ATGCCCGATC TTAAGGTCAC CCAGAAGCTG GCCCAGAAGC TCGGGCACGA CGACACCCGC 
CGCCTGCTGG CGGATAGGAA GCTGGTCCTG CTTGTCGATC TCGATCAGAC GGTGATCCAC 
ACCACCAACG ACACTGTGCC GGACAACATC AAGGGCATCT ACCACTTCCA GCTGTACGGC 
CCACATTCCC CGTGGTACCA CACGCGCCTG CGCCCCGGCA CCGCCGAGTT TCTGGAGCGC 
ATGTCCCAGC TGTACGAACT GCACATTTGC ACTTTTGGTG CGCGTAACTA CGCGCACATG 
ATTGCCCAGC TGCTGGACCC GGAGGGCAAG TTCTTCTCGC ACAGGATTCT GTCCAGAGAC 
GAGTGTTTCA ACGCCACCAG CAAGACGGAC AATTTAAAGG CTCTGTTTCC GAATGGCGAT 
TCCATGGTGT GCATCATTGA CGATCGCGAG GATGTGTGGA ACATGGCTTC AAATTTGATT 
CAGGTTAAGC CCTACCACTT CTTTCAACAC ACTGGTGACA TCAATGCCCC GCCGGGTTTG 
TCTAAGCATG AACTGGATGG CGAAGGCGTT GACTTTAAAG AGATTACCGA GAAGCATGGG 
GACAAAGATA AGACGGAATC CAGCAGCGAA GTCAAACCAG AAGACACCGA TAAGGGTGAT 
AATACCGTCA CAAGCACAAG CAAAGATGAC GATGTAAACG AGGAGTCAGT AGACGTGTTC 
GAAATAGAGG GCGATGCCAA GGATCCAGAG GTTTCCAACG CCTCAAGCGC TACAGAAGCA 
CCCAAAGAGC CGAGAGACAA ATTGAATGGC AAGACTAATG CTGAGGATAT TGTGGTCATA 
GACGACAGCT CATCTGGCTC ACCAGATGCT GAAAAAGCAG CCAGCGATGG CGAAGACGTA 
GTAGTTATTG ATGATAATTC CAAGGAGAGC ACAAAAGCCG AGGTGCCCCC CACACCGGCA 
GAAAAGAATG AGGTTGTAGC CTCATCCACC ACTTCGCCGG ATGAGAAGCG CCCGTCTGCT 
GACGCTGATG TGGCCACCAC GTCTAAGACG CCCAGCTTAC GAGCACCGCT CGAGGGGCAA 
AAGCAGATAG AGATTGAAGA CCCCGACGAT TACCTGCTGT ATCTAGAGGT CATACTTCGT 
AACATTCACA AGCGATTCTA CTCTATTTAC GACGAAACCA CGGAAATACC CGATCTAAAG 
GTTATCGTTC CGAAGATTCG GAGCGAAGTG CTGCGCGGCA AGAACCTGGT GTTCTCCGGC 
CTGGTGCCCA CGCAGATGAA GCTCGAGCAG TCGCGGGCGT ATTTCATAGC CAAGAGCCTG 
GGCGCCGAAG TGAAGCCCAA TATTGACAAG GAGATAACGC ATCTAGTGGC GGTCAACGCA 
GGCACCTACA AGGTTAACGC CGCCAAAAAA GAGCCTGCCA TTAAAGTGGT CAATGCGAAT 
TGGTTGTGGA CCTGCGCCGA GCGCTGGGAG CACGTGGAGG AGAAGCTCTT TCCACTGGAC 
CGCAAGGTAC GCAACAAGGG TCGCCAGCCA CCCGCCCATT GCCATAGTCC CGAGCACGTG 
GTTAACTACA GCGAGCGTTC GGAGATCTCA CCCTCGAGCA GCAAGCAGCA GGAGGAGCAG 
AGCGGCAACT TCCGCGAAAC GCTGAATCCG CTGCTGGTCT TCACCAACGC AGATATCGAG 
TCTATGAATA AGGACTACGA AACATTCTTC GAGTCAGACT CGTCCAGCGA CGAGGGTCCT 
GTAAATTTTG AAAATCCACC AATGGACAAG AAGCTGTTGA AGAGAAAGCG CGAAGATGAT 
AATTCCAACA GAGCTCACGA CTTTTTCACG CGAAGCGATG ATATAATGAT TGGAGCGCCA 
AACCTCGTGG AGGTGGACAT TAGCTCGAAC GAGGAGGCCG ATGACAATAA CGAGAAGGAG 
GACGATGACG ATGAGATGCC CAGCGCCAAG TTTAGGCGAG GGGAAGACCT GCCTTCCGAC 
CTGGAGCTTG GATCCGAGTC CAACAGCGAG AAGGAACCGG AGGACGAGGA CGACGGCGAA 
TGGAACATGA TGGGCGCCGC TCTAGAAAGG GAGTTCCTCG GCCTGGAGGA CTTTGACATG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286846.1
CDS	298..2940
Translation	MQNIPDEEGATPSRAPGGVAASGAAEGDDGGGGSGAKNSASGNSNNNTSGGGIIVLRAALGENEARAVINKWRVREGQFVSAAQILFLYQPVGVDAKDAKDAGKPGGDCAIQRYKSQRAGVVKKRLRKEGELLTKGDAILELSECIHTTVIKDMCADCGADLRQNENGQTSEASVPMVHTMPDLKVTQKLAQKLGHDDTRRLLADRKLVLLVDLDQTVIHTTNDTVPDNIKGIYHFQLYGPHSPWYHTRLRPGTAEFLERMSQLYELHICTFGARNYAHMIAQLLDPEGKFFSHRILSRDECFNATSKTDNLKALFPNGDSMVCIIDDREDVWNMASNLIQVKPYHFFQHTGDINAPPGLSKHELDGEGVDFKEITEKHGDKDKTESSSEVKPEDTDKGDNTVTSTSKDDDVNEESVDVFEIEGDAKDPEVSNASSATEAPKEPRDKLNGKTNAEDIVVIDDSSSGSPDAEKAASDGEDVVVIDDNSKESTKAEVPPTPAEKNEVVASSTTSPDEKRPSADADVATTSKTPSLRAPLEGQKQIEIEDPDDYLLYLEVILRNIHKRFYSIYDETTEIPDLKVIVPKIRSEVLRGKNLVFSGLVPTQMKLEQSRAYFIAKSLGAEVKPNIDKEITHLVAVNAGTYKVNAAKKEPAIKVVNANWLWTCAERWEHVEEKLFPLDRKVRNKGRQPPAHCHSPEHVVNYSERSEISPSSSKQQEEQSGNFRETLNPLLVFTNADIESMNKDYETFFESDSSSDEGPVNFENPPMDKKLLKRKREDDNSNRAHDFFTRSDDIMIGAPNLVEVDISSNEEADDNNEKEDDDDEMPSAKFRRGEDLPSDLELGSESNSEKEPEDEDDGEWNMMGAALEREFLGLEDFDM

Target ORF information:

RefSeq Version	NM_001299917.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster TFIIF-interacting CTD phosphatase (Fcp1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299917.1

ORF Insert Sequence:

ATGCAGAACA TACCGGACGA GGAGGGCGCG ACGCCCAGCC GCGCGCCAGG GGGCGTGGCC 
GCATCTGGAG CTGCCGAAGG CGACGATGGC GGCGGAGGAA GCGGAGCCAA GAACAGCGCC 
AGCGGCAACA GCAACAACAA CACCAGCGGT GGCGGCATCA TTGTTTTGAG AGCTGCGCTG 
GGAGAGAACG AGGCGAGAGC GGTAATCAAT AAATGGCGGG TTCGCGAGGG ACAATTCGTG 
TCGGCGGCCC AAATCTTGTT TCTTTACCAG CCCGTGGGCG TGGACGCGAA GGACGCGAAG 
GACGCGGGAA AGCCGGGCGG GGACTGTGCC ATCCAGAGGT ACAAGTCCCA GCGCGCCGGA 
GTGGTGAAGA AGCGCCTTCG CAAGGAAGGG GAGCTTCTTA CGAAGGGGGA TGCCATTCTG 
GAGCTGTCCG AGTGCATACA CACCACGGTC ATCAAGGACA TGTGCGCGGA CTGCGGAGCC 
GACTTGCGTC AGAACGAGAA TGGACAGACC TCCGAGGCCT CGGTGCCAAT GGTGCACACC 
ATGCCCGATC TTAAGGTCAC CCAGAAGCTG GCCCAGAAGC TCGGGCACGA CGACACCCGC 
CGCCTGCTGG CGGATAGGAA GCTGGTCCTG CTTGTCGATC TCGATCAGAC GGTGATCCAC 
ACCACCAACG ACACTGTGCC GGACAACATC AAGGGCATCT ACCACTTCCA GCTGTACGGC 
CCACATTCCC CGTGGTACCA CACGCGCCTG CGCCCCGGCA CCGCCGAGTT TCTGGAGCGC 
ATGTCCCAGC TGTACGAACT GCACATTTGC ACTTTTGGTG CGCGTAACTA CGCGCACATG 
ATTGCCCAGC TGCTGGACCC GGAGGGCAAG TTCTTCTCGC ACAGGATTCT GTCCAGAGAC 
GAGTGTTTCA ACGCCACCAG CAAGACGGAC AATTTAAAGG CTCTGTTTCC GAATGGCGAT 
TCCATGGTGT GCATCATTGA CGATCGCGAG GATGTGTGGA ACATGGCTTC AAATTTGATT 
CAGGTTAAGC CCTACCACTT CTTTCAACAC ACTGGTGACA TCAATGCCCC GCCGGGTTTG 
TCTAAGCATG AACTGGATGG CGAAGGCGTT GACTTTAAAG AGATTACCGA GAAGCATGGG 
GACAAAGATA AGACGGAATC CAGCAGCGAA GTCAAACCAG AAGACACCGA TAAGGGTGAT 
AATACCGTCA CAAGCACAAG CAAAGATGAC GATGTAAACG AGGAGTCAGT AGACGTGTTC 
GAAATAGAGG GCGATGCCAA GGATCCAGAG GTTTCCAACG CCTCAAGCGC TACAGAAGCA 
CCCAAAGAGC CGAGAGACAA ATTGAATGGC AAGACTAATG CTGAGGATAT TGTGGTCATA 
GACGACAGCT CATCTGGCTC ACCAGATGCT GAAAAAGCAG CCAGCGATGG CGAAGACGTA 
GTAGTTATTG ATGATAATTC CAAGGAGAGC ACAAAAGCCG AGGTGCCCCC CACACCGGCA 
GAAAAGAATG AGGTTGTAGC CTCATCCACC ACTTCGCCGG ATGAGAAGCG CCCGTCTGCT 
GACGCTGATG TGGCCACCAC GTCTAAGACG CCCAGCTTAC GAGCACCGCT CGAGGGGCAA 
AAGCAGATAG AGATTGAAGA CCCCGACGAT TACCTGCTGT ATCTAGAGGT CATACTTCGT 
AACATTCACA AGCGATTCTA CTCTATTTAC GACGAAACCA CGGAAATACC CGATCTAAAG 
GTTATCGTTC CGAAGATTCG GAGCGAAGTG CTGCGCGGCA AGAACCTGGT GTTCTCCGGC 
CTGGTGCCCA CGCAGATGAA GCTCGAGCAG TCGCGGGCGT ATTTCATAGC CAAGAGCCTG 
GGCGCCGAAG TGAAGCCCAA TATTGACAAG GAGATAACGC ATCTAGTGGC GGTCAACGCA 
GGCACCTACA AGGTTAACGC CGCCAAAAAA GAGCCTGCCA TTAAAGTGGT CAATGCGAAT 
TGGTTGTGGA CCTGCGCCGA GCGCTGGGAG CACGTGGAGG AGAAGCTCTT TCCACTGGAC 
CGCAAGGTAC GCAACAAGGG TCGCCAGCCA CCCGCCCATT GCCATAGTCC CGAGCACGTG 
GTTAACTACA GCGAGCGTTC GGAGATCTCA CCCTCGAGCA GCAAGCAGCA GGAGGAGCAG 
AGCGGCAACT TCCGCGAAAC GCTGAATCCG CTGCTGGTCT TCACCAACGC AGATATCGAG 
TCTATGAATA AGGACTACGA AACATTCTTC GAGTCAGACT CGTCCAGCGA CGAGGGTCCT 
GTAAATTTTG AAAATCCACC AATGGACAAG AAGCTGTTGA AGAGAAAGCG CGAAGATGAT 
AATTCCAACA GAGCTCACGA CTTTTTCACG CGAAGCGATG ATATAATGAT TGGAGCGCCA 
AACCTCGTGG AGGTGGACAT TAGCTCGAAC GAGGAGGCCG ATGACAATAA CGAGAAGGAG 
GACGATGACG ATGAGATGCC CAGCGCCAAG TTTAGGCGAG GGGAAGACCT GCCTTCCGAC 
CTGGAGCTTG GATCCGAGTC CAACAGCGAG AAGGAACCGG AGGACGAGGA CGACGGCGAA 
TGGAACATGA TGGGCGCCGC TCTAGAAAGG GAGTTCCTCG GCCTGGAGGA CTTTGACATG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Fcp1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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