DNApol-gamma35 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	DNApol-gamma35
Entrez Gene ID	3772064
Full Name	DNA polymerase gamma 35kD
Synonyms	BG:DS00941.9,CG33084,CG33650,CG8969,D-pol gamma-beta,DNA pol-gam.35,DNApol-gamma,DNApol-gamma.35,Dmel\CG33650,MtPolB,Pol gamma,PolG,Polg,Polgamma,br16,l(2)34De,l(2)br16,pol gamma-beta,pol gamma35,polgamma,polgamma-beta
General protein information	Preferred Names DNA polymerase gamma 35kD Names CG33650 gene product from transcript CG33650-RA CG33650-PA DNA polymerase -gamma DNA polymerase gamma DNApol-gamma35-PA accessory subunit of mitochondrial DNA polymerase accessory(beta)subunit beta subunit lethal 16 in the black-reduced region lethal(2)34De mitochondrial DNA polymerase accessory subunit mitochondrial gamma polymerase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 34D1-34D1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001032091.3	NP_001027262.1	DNA polymerase gamma 35kD

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The stimulatory effect of mtSSB on Pol gamma on these ssDNA templates is not species-specific.
Title: Mitochondrial Single-stranded DNA-binding Proteins Stimulate the Activity of DNA Polymerase ? by Organization of the Template DNA.

mtSSB uses distinct structural elements to interact functionally with its mtDNA replisome partners and to promote proper mtDNA replication in animal cells
Title: Reduced stimulation of recombinant DNA polymerase ? and mitochondrial DNA (mtDNA) helicase by variants of mitochondrial single-stranded DNA-binding protein (mtSSB) correlates with defects in mtDNA replication in animal cells.

The following DNApol-gamma35 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DNApol-gamma35 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa15595	NM_001032091.3 Latest version!	Drosophila melanogaster DNA polymerase gamma 35kD (DNApol-gamma35), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DNApol-gamma35 ( NM_001032091.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001027262.1 Drosophila melanogaster DNA polymerase gamma 35kD (DNApol-gamma35), mRNA.

Price & Availability↑

CloneID	OFa15595
Clone ID Related Accession (Same CDS sequence)	NM_001032091.3
Accession Version	NM_001032091.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1086bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	DNA polymerase gamma 35kD
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001032091.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTCGCA TACAACGATG CTTTAAGTCC CTGGCTTCCG CTGGCTTCTT TCGCACCGTG 
GAAGACAATA AACTGGAGCT ACTGAGTCAT GGAAGGGAAT ACGCCAAACT GTTGCAACAG 
CATTGGACAC GACTACGTCC CTTGGCTGCG CACCTGGGCG CCACGAAAGA ACCCATAAAT 
CCAGTCAACA TCCAGCGTTT TTCCTTTCCA CAAAGCCAGC AATTCCGTAA CAATTTCCAA 
AAACTCGTCA AAGATCATCC TCGAAAGGCC AAATGTCCCA CTCTTCTGAA ACATCAAAGC 
ACTTGTTCTG GTCCCACTAG CAATTCCCTA TTTGGGATAA AGGGTCCTAC GCTACATTTA 
ACCACGGACT TCCTAGTGGA ACCACATCGC GCACTGGAGC ACTTCTACAA CATGCAGCGT 
GAGAGCAAGA TCTGGTGGAT GCGTCTTTCC TCGAATCCCA GTCGCTACCG CATCGTACCT 
TGCGATTTGG CTGAGGATCT CAATCCCAAC GACTATCAAG CCATCGATAT CCGCACGAGC 
TACGGTGATG CAGGAGAAGT GACCGTGGAG CAACTGAGCC TGGTGAGAAT AGTGGACGAC 
AAAGACTTCC GGCTGCCCGA TGCGCGAACG GGTGAAATTG TGCAACCGAC TGTCATAAGG 
TCTGTGATAG AGCTGGAAAC TACCACCTGC GCTCTGCTTC TGGATGGCTG TGATCATGGC 
CGGGACTCGC AATCCCTGCT CTTACACCGC GTTCTGGCGC CATATCAATG TGGAATTGCC 
TGTGTGGAAA GTGATTCCGA GCTATCTGCT GACTTGTCTG ATCTTTGTCA GCATCTGAAA 
CATGTTCTCA ACCACGCCGG TCTTAGACTT TCTGAAGGGG ATGGAATTCG TACGACGAAG 
AACGCTTCTC ATTTAGCAGA GCACTTGCTG GAAACGGATA TGCTCGGCAT ACCTTATACA 
CTTGTTATAA ACGAGCAAAC GCTAAGAAAC GGACTGATGC AGTTGCGCAG CCGGGACACA 
AGGCTGGCGG AGACCATACA CATAAGCGAT GTACCGGACT ATTTATTAAA TATATTTAAA 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001027262.1
CDS	478..1563
Translation	MSRIQRCFKSLASAGFFRTVEDNKLELLSHGREYAKLLQQHWTRLRPLAAHLGATKEPINPVNIQRFSFPQSQQFRNNFQKLVKDHPRKAKCPTLLKHQSTCSGPTSNSLFGIKGPTLHLTTDFLVEPHRALEHFYNMQRESKIWWMRLSSNPSRYRIVPCDLAEDLNPNDYQAIDIRTSYGDAGEVTVEQLSLVRIVDDKDFRLPDARTGEIVQPTVIRSVIELETTTCALLLDGCDHGRDSQSLLLHRVLAPYQCGIACVESDSELSADLSDLCQHLKHVLNHAGLRLSEGDGIRTTKNASHLAEHLLETDMLGIPYTLVINEQTLRNGLMQLRSRDTRLAETIHISDVPDYLLNIFKN

Target ORF information:

RefSeq Version	NM_001032091.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster DNA polymerase gamma 35kD (DNApol-gamma35), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001032091.3

ORF Insert Sequence:

ATGAGTCGCA TACAACGATG CTTTAAGTCC CTGGCTTCCG CTGGCTTCTT TCGCACCGTG 
GAAGACAATA AACTGGAGCT ACTGAGTCAT GGAAGGGAAT ACGCCAAACT GTTGCAACAG 
CATTGGACAC GACTACGTCC CTTGGCTGCG CACCTGGGCG CCACGAAAGA ACCCATAAAT 
CCAGTCAACA TCCAGCGTTT TTCCTTTCCA CAAAGCCAGC AATTCCGTAA CAATTTCCAA 
AAACTCGTCA AAGATCATCC TCGAAAGGCC AAATGTCCCA CTCTTCTGAA ACATCAAAGC 
ACTTGTTCTG GTCCCACTAG CAATTCCCTA TTTGGGATAA AGGGTCCTAC GCTACATTTA 
ACCACGGACT TCCTAGTGGA ACCACATCGC GCACTGGAGC ACTTCTACAA CATGCAGCGT 
GAGAGCAAGA TCTGGTGGAT GCGTCTTTCC TCGAATCCCA GTCGCTACCG CATCGTACCT 
TGCGATTTGG CTGAGGATCT CAATCCCAAC GACTATCAAG CCATCGATAT CCGCACGAGC 
TACGGTGATG CAGGAGAAGT GACCGTGGAG CAACTGAGCC TGGTGAGAAT AGTGGACGAC 
AAAGACTTCC GGCTGCCCGA TGCGCGAACG GGTGAAATTG TGCAACCGAC TGTCATAAGG 
TCTGTGATAG AGCTGGAAAC TACCACCTGC GCTCTGCTTC TGGATGGCTG TGATCATGGC 
CGGGACTCGC AATCCCTGCT CTTACACCGC GTTCTGGCGC CATATCAATG TGGAATTGCC 
TGTGTGGAAA GTGATTCCGA GCTATCTGCT GACTTGTCTG ATCTTTGTCA GCATCTGAAA 
CATGTTCTCA ACCACGCCGG TCTTAGACTT TCTGAAGGGG ATGGAATTCG TACGACGAAG 
AACGCTTCTC ATTTAGCAGA GCACTTGCTG GAAACGGATA TGCTCGGCAT ACCTTATACA 
CTTGTTATAA ACGAGCAAAC GCTAAGAAAC GGACTGATGC AGTTGCGCAG CCGGGACACA 
AGGCTGGCGG AGACCATACA CATAAGCGAT GTACCGGACT ATTTATTAAA TATATTTAAA 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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DNApol-gamma35 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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