robo1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	robo1
Entrez Gene ID	37603
Full Name	roundabout 1
Synonyms	BEST:GM07780,CG13521,CT32892,D-Robo1,Dmel\CG13521,ROBO,Robo,Robo-1,Robo1,dRobo,dRobo1,robo,robo1S
General protein information	Preferred Names roundabout 1 Names CG13521 gene product from transcript CG13521-RA CG13521-PA CG13521-PC robo robo1-PA robo1-PC roundabout
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 59A2-59A3

mRNA and Protein(s)

mRNA	Protein	Name
NM_057551.4	NP_476899.1	roundabout 1, isoform A
NM_166543.2	NP_726224.3	roundabout 1, isoform C

Pathways from BioSystems

Homologies

Homo sapiens (human)	ROBO2	NP_002933.1
Rattus norvegicus (Norway rat)	Robo2	NP_115289.1
Canis lupus familiaris (dog)	LOC487691	XP_005638813.1
Bos taurus (cattle)	LOC534842	XP_002684698.3
Gallus gallus (chicken)	ROBO2	XP_416674.4
Danio rerio (zebrafish)	robo2	NP_571708.1
	LOC719197	XP_002802664.1
Mus musculus (house mouse)	Robo2	NP_780758.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010969	XP_309726.4
Drosophila melanogaster (fruit fly)	robo	NP_476899.1
Xenopus tropicalis (tropical clawed frog)	robo2	XP_004912137.1
Pan troglodytes (chimpanzee)	ROBO2	XP_516591.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

loss of Atg16 or its binding partner Rab19 impairs Slit production, which normally inhibits enteroendocrine cell generation by activating Robo signaling in stem cells.

Findings indicate that four of the five immunoglobulin-like (Ig) domains within Drosophila Robo1 are dispensable for its role in midline repulsion, despite their strong evolutionary conservation, and highlight a unique requirement for the Slit-binding Ig1 domain in the regulation of midline crossing.
Title: In vivo functional analysis of Drosophila Robo1 immunoglobulin-like domains.

FruBM employs the Robo1 pathway for formation of the male-specific ipsilateral neurite
Title: Fruitless Represses robo1 Transcription to Shape Male-Specific Neural Morphology and Behavior in Drosophila.

data support a model in which Robo's ligand-dependent internalization from cell surface to late endosome is essential for receptor activation and proper repulsive guidance at the midline by allowing recruitment of the downstream effector Son of Sevenless
Title: Slit-Dependent Endocytic Trafficking of the Robo Receptor Is Required for Son of Sevenless Recruitment and Midline Axon Repulsion.

Deleting Ig1 from Robo1 disrupts Slit binding in cultured Drosophila cells. Robo1 variant lacking Ig1 (Robo1Ig1) is unable to promote ectopic midline repulsion.
Title: Slit Binding via the Ig1 Domain Is Essential for Midline Repulsion by Drosophila Robo1 but Dispensable for Receptor Expression, Localization, and Regulation in Vivo.

The following robo1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the robo1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa12568	NM_166543.2 Latest version!	Drosophila melanogaster roundabout 1 (robo1), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa12568	NM_057551.4 Latest version!	Drosophila melanogaster roundabout 1 (robo1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

robo1 ( NM_057551.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476899.1 Drosophila melanogaster roundabout 1 (robo1), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa12568
Clone ID Related Accession (Same CDS sequence)	NM_057551.4 , NM_166543.2
Accession Version	NM_057551.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	roundabout 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_057551.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATCCCA TGCATCCCGA AAACCACGCC ATCGCCCGGA GCACGAGCAC CACTAATAAC 
CCATCTCGCA GTCGGAGCAG CAGGATGTGG CTCCTGCCCG CCTGGCTGCT CCTCGTCCTG 
GTGGCCAGCA ATGGCCTGCC AGCAGTCAGA GGCCAGTACC AATCGCCACG TATCATCGAG 
CATCCCACGG ATCTGGTCGT TAAGAAGAAT GAACCCGCCA CGCTCAACTG CAAAGTGGAG 
GGCAAGCCGG AACCCACCAT TGAGTGGTTT AAGGATGGCG AACCCGTCAG CACCAACGAA 
AAGAAATCGC ACCGCGTCCA GTTCAAGGAC GGCGCCCTCT TCTTCTACAG GACAATGCAG 
GGCAAGAAGG AACAGGACGG CGGAGAGTAC TGGTGCGTGG CCAAGAACCG AGTGGGCCAG 
GCCGTTAGTC GCCATGCCTC GCTCCAGATA GCTGTTTTGC GCGACGATTT TCGCGTGGAG 
CCCAAAGACA CGCGAGTGGC CAAAGGCGAG ACGGCTCTGC TGGAGTGTGG GCCGCCCAAA 
GGCATTCCAG AGCCAACGCT GATTTGGATA AAGGACGGCG TTCCCTTGGA CGACCTGAAA 
GCCATGTCGT TTGGCGCCAG CTCCCGCGTT CGAATTGTGG ACGGTGGCAA CCTGCTGATC 
AGCAATGTGG AGCCCATTGA TGAGGGCAAC TACAAGTGCA TTGCCCAGAA TCTGGTAGGC 
ACCCGCGAGA GCAGCTATGC CAAGCTGATT GTCCAGGTCA AACCATACTT TATGAAGGAG 
CCCAAGGATC AGGTGATGCT CTACGGCCAG ACAGCCACTT TCCACTGCTC AGTGGGCGGT 
GATCCGCCGC CGAAAGTGTT GTGGAAAAAG GAGGAGGGCA ATATTCCGGT GTCCAGAGCG 
CGAATCCTTC ACGACGAGAA AAGTTTAGAG ATATCCAACA TAACGCCCAC CGATGAGGGC 
ACCTATGTCT GCGAGGCACA CAACAATGTC GGTCAGATCA GCGCTAGGGC TTCTCTTATA 
GTCCATGCTC CGCCGAACTT TACGAAAAGA CCCAGTAACA AGAAAGTGGG ACTAAATGGT 
GTTGTCCAAC TACCTTGCAT GGCCTCCGGA AACCCACCGC CATCTGTTTT CTGGACCAAG 
GAAGGAGTAT CCACTCTCAT GTTCCCAAAT AGTTCGCACG GGAGGCAGCA TGTGGCTGCC 
GATGGAACTC TGCAGATTAC GGATGTGCGG CAGGAAGACG AAGGCTACTA TGTGTGTTCC 
GCTTTCAGTG TAGTCGATTC CTCTACAGTA CGGGTTTTCC TGCAAGTCAG CTCGTTAGAC 
GAGCGTCCAC CTCCGATTAT TCAAATCGGA CCTGCCAATC AAACACTGCC CAAGGGATCA 
GTTGCTACTT TACCCTGTCG GGCCACCGGA AATCCCAGTC CCCGTATTAA GTGGTTCCAC 
GATGGACATG CCGTACAAGC GGGCAATCGA TACAGCATCA TCCAAGGAAG CTCACTGAGA 
GTCGATGACC TTCAACTTAG TGACTCTGGT ACCTACACCT GCACTGCATC TGGCGAACGA 
GGAGAAACTT CCTGGGCTGC CACACTAACG GTGGAAAAAC CCGGTTCTAC ATCTCTTCAC 
CGGGCAGCTG ATCCTAGCAC TTATCCTGCT CCTCCAGGAA CACCTAAAGT CCTGAATGTC 
AGTCGCACCA GCATTAGTCT TCGTTGGGCT AAAAGCCAAG AGAAACCCGG AGCTGTGGGC 
CCAATCATTG GATACACTGT AGAGTACTTC AGTCCGGATC TGCAAACTGG TTGGATTGTG 
GCTGCCCAAC GAGTTGGCGA CACTCAAGTC ACTATCTCGG GTCTCACTCC TGGCACTTCG 
TATGTGTTCC TAGTTAGAGC TGAGAATACT CAGGGTATTT CTGTGCCTTC CGGCTTATCA 
AATACTATTA AAACCATTGA GGCGGATTTC GATGCAGCTT CTGCCAATGA TTTGTCAGCA 
GCTCGAACTT TGCTGACGGG AAAGTCGGTG GAGCTAATAG ATGCCTCGGC TATCAATGCT 
AGTGCCGTAA GACTTGAGTG GATGCTCCAC GTGAGCGCTG ATGAGAAATA TGTAGAGGGC 
CTGCGCATAC ACTATAAGGA TGCCAGTGTA CCATCCGCAC AGTATCACTC GATCACTGTT 
ATGGATGCCT CTGCAGAATC GTTTGTGGTG GGAAACCTTA AGAAGTACAC CAAGTATGAG 
TTCTTCCTAA CACCCTTTTT TGAGACAATT GAAGGACAGC CCAGTAACTC CAAGACAGCC 
CTCACCTATG AAGATGTTCC CTCCGCACCA CCGGATAACA TTCAGATTGG CATGTACAAC 
CAAACAGCCG GTTGGGTGCG TTGGACTCCG CCACCCTCCC AGCACCACAA TGGCAATTTG 
TATGGCTACA AGATTGAGGT CAGCGCCGGT AACACCATGA AGGTGCTGGC CAATATGACT 
CTTAATGCTA CCACCACATC TGTGCTCCTA AATAACCTAA CCACCGGAGC TGTGTACAGC 
GTGAGGTTGA ACTCCTTTAC CAAGGCAGGA GATGGACCTT ACTCCAAACC GATATCACTA 
TTCATGGACC CCACCCATCA TGTGCATCCG CCACGGGCAC ATCCAAGCGG CACCCATGAT 
GGGCGACATG AGGGACAGGA TCTCACGTAT CATAACAATG GCAACATACC ACCTGGCGAC 
ATTAATCCCA CCACTCATAA AAAGACCACT GACTACCTAT CTGGACCATG GCTAATGGTG 
CTGGTCTGCA TCGTTCTTCT AGTCCTGGTT ATTTCGGCGG CTATTTCGAT GGTCTACTTC 
AAGCGCAAGC ATCAAATGAC CAAGGAATTG GGTCACTTAA GTGTGGTCAG TGACAACGAA 
ATAACCGCAT TGAACATCAA TAGCAAAGAG AGCCTTTGGA TAGACCATCA TCGTGGATGG 
CGAACTGCCG ATACTGACAA AGACTCAGGA TTAAGCGAAT CGAAGCTACT ATCCCACGTA 
AACAGCAGCC AATCCAACTA CAATAACTCC GATGGAGGAA CCGATTATGC AGAAGTTGAC 
ACCCGTAACC TTACCACCTT CTACAATTGT CGCAAGAGCC CCGATAATCC CACGCCGTAC 
GCCACCACTA TGATCATTGG TACCTCTTCC AGTGAGACCT GCACCAAGAC AACATCTATA 
AGTGCCGATA AGGACTCGGG AACTCATTCG CCCTATTCTG ACGCATTTGC CGGTCAGGTG 
CCAGCGGTTC CTGTTGTCAA ATCCAACTAT CTTCAGTATC CGGTTGAACC GATCAACTGG 
TCAGAGTTTC TACCCCCGCC GCCAGAACAC CCACCTCCGT CTTCTACCTA TGGATACGCA 
CAAGGATCTC CTGAATCTTC ACGGAAGAGC TCCAAAAGCG CAGGTTCCGG CATTTCTACA 
AATCAAAGCA TTCTGAACGC CTCCATACAC AGCAGCTCCT CGGGCGGCTT TTCAGCTTGG 
GGAGTTTCGC CCCAATATGC TGTCGCCTGT CCACCGGAAA ACGTTTATAG CAATCCGCTG 
TCGGCAGTGG CTGGCGGCAC CCAGAACCGC TATCAGATAA CGCCAACAAA CCAACATCCG 
CCACAGTTAC CGGCCTACTT TGCCACCACG GGTCCAGGAG GAGCTGTACC ACCCAACCAC 
CTGCCATTTG CCACACAGCG TCATGCAGCC AGCGAGTACC AGGCTGGACT GAATGCAGCG 
CGATGTGCCC AAAGCCGCGC CTGCAACAGC TGCGATGCCT TGGCCACACC CTCGCCCATG 
CAACCCCCAC CGCCAGTTCC CGTACCCGAG GGCTGGTACC AACCGGTGCA TCCCAATAGC 
CACCCGATGC ACCCGACCTC CTCCAACCAC CAGATCTACC AGTGCTCCTC CGAGTGCTCG 
GATCACTCGA GGAGCTCGCA GAGTCACAAG CGGCAGCTGC AGCTCGAGGA GCACGGCAGC 
AGTGCCAAAC AACGCGGAGG ACACCACCGT CGACGAGCCC CGGTGGTGCA GCCGTGCATG 
GAGAGCGAGA ACGAGAACAT GCTGGCGGAG TACGAGCAGC GCCAGTACAC CAGCGATTGT 
TGCAATAGCT CCCGCGAGGG CGACACCTGC TCCTGCAGCG AGGGATCCTG TCTTTACGCC 
GAGGCGGGCG AGCCGGCGCC TCGTCAAATG ACTGCTAAGA ACACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476899.1
CDS	176..4363
Translation	MHPMHPENHAIARSTSTTNNPSRSRSSRMWLLPAWLLLVLVASNGLPAVRGQYQSPRIIEHPTDLVVKKNEPATLNCKVEGKPEPTIEWFKDGEPVSTNEKKSHRVQFKDGALFFYRTMQGKKEQDGGEYWCVAKNRVGQAVSRHASLQIAVLRDDFRVEPKDTRVAKGETALLECGPPKGIPEPTLIWIKDGVPLDDLKAMSFGASSRVRIVDGGNLLISNVEPIDEGNYKCIAQNLVGTRESSYAKLIVQVKPYFMKEPKDQVMLYGQTATFHCSVGGDPPPKVLWKKEEGNIPVSRARILHDEKSLEISNITPTDEGTYVCEAHNNVGQISARASLIVHAPPNFTKRPSNKKVGLNGVVQLPCMASGNPPPSVFWTKEGVSTLMFPNSSHGRQHVAADGTLQITDVRQEDEGYYVCSAFSVVDSSTVRVFLQVSSLDERPPPIIQIGPANQTLPKGSVATLPCRATGNPSPRIKWFHDGHAVQAGNRYSIIQGSSLRVDDLQLSDSGTYTCTASGERGETSWAATLTVEKPGSTSLHRAADPSTYPAPPGTPKVLNVSRTSISLRWAKSQEKPGAVGPIIGYTVEYFSPDLQTGWIVAAQRVGDTQVTISGLTPGTSYVFLVRAENTQGISVPSGLSNTIKTIEADFDAASANDLSAARTLLTGKSVELIDASAINASAVRLEWMLHVSADEKYVEGLRIHYKDASVPSAQYHSITVMDASAESFVVGNLKKYTKYEFFLTPFFETIEGQPSNSKTALTYEDVPSAPPDNIQIGMYNQTAGWVRWTPPPSQHHNGNLYGYKIEVSAGNTMKVLANMTLNATTTSVLLNNLTTGAVYSVRLNSFTKAGDGPYSKPISLFMDPTHHVHPPRAHPSGTHDGRHEGQDLTYHNNGNIPPGDINPTTHKKTTDYLSGPWLMVLVCIVLLVLVISAAISMVYFKRKHQMTKELGHLSVVSDNEITALNINSKESLWIDHHRGWRTADTDKDSGLSESKLLSHVNSSQSNYNNSDGGTDYAEVDTRNLTTFYNCRKSPDNPTPYATTMIIGTSSSETCTKTTSISADKDSGTHSPYSDAFAGQVPAVPVVKSNYLQYPVEPINWSEFLPPPPEHPPPSSTYGYAQGSPESSRKSSKSAGSGISTNQSILNASIHSSSSGGFSAWGVSPQYAVACPPENVYSNPLSAVAGGTQNRYQITPTNQHPPQLPAYFATTGPGGAVPPNHLPFATQRHAASEYQAGLNAARCAQSRACNSCDALATPSPMQPPPPVPVPEGWYQPVHPNSHPMHPTSSNHQIYQCSSECSDHSRSSQSHKRQLQLEEHGSSAKQRGGHHRRRAPVVQPCMESENENMLAEYEQRQYTSDCCNSSREGDTCSCSEGSCLYAEAGEPAPRQMTAKNT

Target ORF information:

RefSeq Version	NM_057551.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster roundabout 1 (robo1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057551.4

ORF Insert Sequence:

ATGCATCCCA TGCATCCCGA AAACCACGCC ATCGCCCGGA GCACGAGCAC CACTAATAAC 
CCATCTCGCA GTCGGAGCAG CAGGATGTGG CTCCTGCCCG CCTGGCTGCT CCTCGTCCTG 
GTGGCCAGCA ATGGCCTGCC AGCAGTCAGA GGCCAGTACC AATCGCCACG TATCATCGAG 
CATCCCACGG ATCTGGTCGT TAAGAAGAAT GAACCCGCCA CGCTCAACTG CAAAGTGGAG 
GGCAAGCCGG AACCCACCAT TGAGTGGTTT AAGGATGGCG AACCCGTCAG CACCAACGAA 
AAGAAATCGC ACCGCGTCCA GTTCAAGGAC GGCGCCCTCT TCTTCTACAG GACAATGCAG 
GGCAAGAAGG AACAGGACGG CGGAGAGTAC TGGTGCGTGG CCAAGAACCG AGTGGGCCAG 
GCCGTTAGTC GCCATGCCTC GCTCCAGATA GCTGTTTTGC GCGACGATTT TCGCGTGGAG 
CCCAAAGACA CGCGAGTGGC CAAAGGCGAG ACGGCTCTGC TGGAGTGTGG GCCGCCCAAA 
GGCATTCCAG AGCCAACGCT GATTTGGATA AAGGACGGCG TTCCCTTGGA CGACCTGAAA 
GCCATGTCGT TTGGCGCCAG CTCCCGCGTT CGAATTGTGG ACGGTGGCAA CCTGCTGATC 
AGCAATGTGG AGCCCATTGA TGAGGGCAAC TACAAGTGCA TTGCCCAGAA TCTGGTAGGC 
ACCCGCGAGA GCAGCTATGC CAAGCTGATT GTCCAGGTCA AACCATACTT TATGAAGGAG 
CCCAAGGATC AGGTGATGCT CTACGGCCAG ACAGCCACTT TCCACTGCTC AGTGGGCGGT 
GATCCGCCGC CGAAAGTGTT GTGGAAAAAG GAGGAGGGCA ATATTCCGGT GTCCAGAGCG 
CGAATCCTTC ACGACGAGAA AAGTTTAGAG ATATCCAACA TAACGCCCAC CGATGAGGGC 
ACCTATGTCT GCGAGGCACA CAACAATGTC GGTCAGATCA GCGCTAGGGC TTCTCTTATA 
GTCCATGCTC CGCCGAACTT TACGAAAAGA CCCAGTAACA AGAAAGTGGG ACTAAATGGT 
GTTGTCCAAC TACCTTGCAT GGCCTCCGGA AACCCACCGC CATCTGTTTT CTGGACCAAG 
GAAGGAGTAT CCACTCTCAT GTTCCCAAAT AGTTCGCACG GGAGGCAGCA TGTGGCTGCC 
GATGGAACTC TGCAGATTAC GGATGTGCGG CAGGAAGACG AAGGCTACTA TGTGTGTTCC 
GCTTTCAGTG TAGTCGATTC CTCTACAGTA CGGGTTTTCC TGCAAGTCAG CTCGTTAGAC 
GAGCGTCCAC CTCCGATTAT TCAAATCGGA CCTGCCAATC AAACACTGCC CAAGGGATCA 
GTTGCTACTT TACCCTGTCG GGCCACCGGA AATCCCAGTC CCCGTATTAA GTGGTTCCAC 
GATGGACATG CCGTACAAGC GGGCAATCGA TACAGCATCA TCCAAGGAAG CTCACTGAGA 
GTCGATGACC TTCAACTTAG TGACTCTGGT ACCTACACCT GCACTGCATC TGGCGAACGA 
GGAGAAACTT CCTGGGCTGC CACACTAACG GTGGAAAAAC CCGGTTCTAC ATCTCTTCAC 
CGGGCAGCTG ATCCTAGCAC TTATCCTGCT CCTCCAGGAA CACCTAAAGT CCTGAATGTC 
AGTCGCACCA GCATTAGTCT TCGTTGGGCT AAAAGCCAAG AGAAACCCGG AGCTGTGGGC 
CCAATCATTG GATACACTGT AGAGTACTTC AGTCCGGATC TGCAAACTGG TTGGATTGTG 
GCTGCCCAAC GAGTTGGCGA CACTCAAGTC ACTATCTCGG GTCTCACTCC TGGCACTTCG 
TATGTGTTCC TAGTTAGAGC TGAGAATACT CAGGGTATTT CTGTGCCTTC CGGCTTATCA 
AATACTATTA AAACCATTGA GGCGGATTTC GATGCAGCTT CTGCCAATGA TTTGTCAGCA 
GCTCGAACTT TGCTGACGGG AAAGTCGGTG GAGCTAATAG ATGCCTCGGC TATCAATGCT 
AGTGCCGTAA GACTTGAGTG GATGCTCCAC GTGAGCGCTG ATGAGAAATA TGTAGAGGGC 
CTGCGCATAC ACTATAAGGA TGCCAGTGTA CCATCCGCAC AGTATCACTC GATCACTGTT 
ATGGATGCCT CTGCAGAATC GTTTGTGGTG GGAAACCTTA AGAAGTACAC CAAGTATGAG 
TTCTTCCTAA CACCCTTTTT TGAGACAATT GAAGGACAGC CCAGTAACTC CAAGACAGCC 
CTCACCTATG AAGATGTTCC CTCCGCACCA CCGGATAACA TTCAGATTGG CATGTACAAC 
CAAACAGCCG GTTGGGTGCG TTGGACTCCG CCACCCTCCC AGCACCACAA TGGCAATTTG 
TATGGCTACA AGATTGAGGT CAGCGCCGGT AACACCATGA AGGTGCTGGC CAATATGACT 
CTTAATGCTA CCACCACATC TGTGCTCCTA AATAACCTAA CCACCGGAGC TGTGTACAGC 
GTGAGGTTGA ACTCCTTTAC CAAGGCAGGA GATGGACCTT ACTCCAAACC GATATCACTA 
TTCATGGACC CCACCCATCA TGTGCATCCG CCACGGGCAC ATCCAAGCGG CACCCATGAT 
GGGCGACATG AGGGACAGGA TCTCACGTAT CATAACAATG GCAACATACC ACCTGGCGAC 
ATTAATCCCA CCACTCATAA AAAGACCACT GACTACCTAT CTGGACCATG GCTAATGGTG 
CTGGTCTGCA TCGTTCTTCT AGTCCTGGTT ATTTCGGCGG CTATTTCGAT GGTCTACTTC 
AAGCGCAAGC ATCAAATGAC CAAGGAATTG GGTCACTTAA GTGTGGTCAG TGACAACGAA 
ATAACCGCAT TGAACATCAA TAGCAAAGAG AGCCTTTGGA TAGACCATCA TCGTGGATGG 
CGAACTGCCG ATACTGACAA AGACTCAGGA TTAAGCGAAT CGAAGCTACT ATCCCACGTA 
AACAGCAGCC AATCCAACTA CAATAACTCC GATGGAGGAA CCGATTATGC AGAAGTTGAC 
ACCCGTAACC TTACCACCTT CTACAATTGT CGCAAGAGCC CCGATAATCC CACGCCGTAC 
GCCACCACTA TGATCATTGG TACCTCTTCC AGTGAGACCT GCACCAAGAC AACATCTATA 
AGTGCCGATA AGGACTCGGG AACTCATTCG CCCTATTCTG ACGCATTTGC CGGTCAGGTG 
CCAGCGGTTC CTGTTGTCAA ATCCAACTAT CTTCAGTATC CGGTTGAACC GATCAACTGG 
TCAGAGTTTC TACCCCCGCC GCCAGAACAC CCACCTCCGT CTTCTACCTA TGGATACGCA 
CAAGGATCTC CTGAATCTTC ACGGAAGAGC TCCAAAAGCG CAGGTTCCGG CATTTCTACA 
AATCAAAGCA TTCTGAACGC CTCCATACAC AGCAGCTCCT CGGGCGGCTT TTCAGCTTGG 
GGAGTTTCGC CCCAATATGC TGTCGCCTGT CCACCGGAAA ACGTTTATAG CAATCCGCTG 
TCGGCAGTGG CTGGCGGCAC CCAGAACCGC TATCAGATAA CGCCAACAAA CCAACATCCG 
CCACAGTTAC CGGCCTACTT TGCCACCACG GGTCCAGGAG GAGCTGTACC ACCCAACCAC 
CTGCCATTTG CCACACAGCG TCATGCAGCC AGCGAGTACC AGGCTGGACT GAATGCAGCG 
CGATGTGCCC AAAGCCGCGC CTGCAACAGC TGCGATGCCT TGGCCACACC CTCGCCCATG 
CAACCCCCAC CGCCAGTTCC CGTACCCGAG GGCTGGTACC AACCGGTGCA TCCCAATAGC 
CACCCGATGC ACCCGACCTC CTCCAACCAC CAGATCTACC AGTGCTCCTC CGAGTGCTCG 
GATCACTCGA GGAGCTCGCA GAGTCACAAG CGGCAGCTGC AGCTCGAGGA GCACGGCAGC 
AGTGCCAAAC AACGCGGAGG ACACCACCGT CGACGAGCCC CGGTGGTGCA GCCGTGCATG 
GAGAGCGAGA ACGAGAACAT GCTGGCGGAG TACGAGCAGC GCCAGTACAC CAGCGATTGT 
TGCAATAGCT CCCGCGAGGG CGACACCTGC TCCTGCAGCG AGGGATCCTG TCTTTACGCC 
GAGGCGGGCG AGCCGGCGCC TCGTCAAATG ACTGCTAAGA ACACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo1 ( NM_166543.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726224.3 Drosophila melanogaster roundabout 1 (robo1), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa12568
Clone ID Related Accession (Same CDS sequence)	NM_057551.4 , NM_166543.2
Accession Version	NM_166543.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	roundabout 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166543.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATCCCA TGCATCCCGA AAACCACGCC ATCGCCCGGA GCACGAGCAC CACTAATAAC 
CCATCTCGCA GTCGGAGCAG CAGGATGTGG CTCCTGCCCG CCTGGCTGCT CCTCGTCCTG 
GTGGCCAGCA ATGGCCTGCC AGCAGTCAGA GGCCAGTACC AATCGCCACG TATCATCGAG 
CATCCCACGG ATCTGGTCGT TAAGAAGAAT GAACCCGCCA CGCTCAACTG CAAAGTGGAG 
GGCAAGCCGG AACCCACCAT TGAGTGGTTT AAGGATGGCG AACCCGTCAG CACCAACGAA 
AAGAAATCGC ACCGCGTCCA GTTCAAGGAC GGCGCCCTCT TCTTCTACAG GACAATGCAG 
GGCAAGAAGG AACAGGACGG CGGAGAGTAC TGGTGCGTGG CCAAGAACCG AGTGGGCCAG 
GCCGTTAGTC GCCATGCCTC GCTCCAGATA GCTGTTTTGC GCGACGATTT TCGCGTGGAG 
CCCAAAGACA CGCGAGTGGC CAAAGGCGAG ACGGCTCTGC TGGAGTGTGG GCCGCCCAAA 
GGCATTCCAG AGCCAACGCT GATTTGGATA AAGGACGGCG TTCCCTTGGA CGACCTGAAA 
GCCATGTCGT TTGGCGCCAG CTCCCGCGTT CGAATTGTGG ACGGTGGCAA CCTGCTGATC 
AGCAATGTGG AGCCCATTGA TGAGGGCAAC TACAAGTGCA TTGCCCAGAA TCTGGTAGGC 
ACCCGCGAGA GCAGCTATGC CAAGCTGATT GTCCAGGTCA AACCATACTT TATGAAGGAG 
CCCAAGGATC AGGTGATGCT CTACGGCCAG ACAGCCACTT TCCACTGCTC AGTGGGCGGT 
GATCCGCCGC CGAAAGTGTT GTGGAAAAAG GAGGAGGGCA ATATTCCGGT GTCCAGAGCG 
CGAATCCTTC ACGACGAGAA AAGTTTAGAG ATATCCAACA TAACGCCCAC CGATGAGGGC 
ACCTATGTCT GCGAGGCACA CAACAATGTC GGTCAGATCA GCGCTAGGGC TTCTCTTATA 
GTCCATGCTC CGCCGAACTT TACGAAAAGA CCCAGTAACA AGAAAGTGGG ACTAAATGGT 
GTTGTCCAAC TACCTTGCAT GGCCTCCGGA AACCCACCGC CATCTGTTTT CTGGACCAAG 
GAAGGAGTAT CCACTCTCAT GTTCCCAAAT AGTTCGCACG GGAGGCAGCA TGTGGCTGCC 
GATGGAACTC TGCAGATTAC GGATGTGCGG CAGGAAGACG AAGGCTACTA TGTGTGTTCC 
GCTTTCAGTG TAGTCGATTC CTCTACAGTA CGGGTTTTCC TGCAAGTCAG CTCGTTAGAC 
GAGCGTCCAC CTCCGATTAT TCAAATCGGA CCTGCCAATC AAACACTGCC CAAGGGATCA 
GTTGCTACTT TACCCTGTCG GGCCACCGGA AATCCCAGTC CCCGTATTAA GTGGTTCCAC 
GATGGACATG CCGTACAAGC GGGCAATCGA TACAGCATCA TCCAAGGAAG CTCACTGAGA 
GTCGATGACC TTCAACTTAG TGACTCTGGT ACCTACACCT GCACTGCATC TGGCGAACGA 
GGAGAAACTT CCTGGGCTGC CACACTAACG GTGGAAAAAC CCGGTTCTAC ATCTCTTCAC 
CGGGCAGCTG ATCCTAGCAC TTATCCTGCT CCTCCAGGAA CACCTAAAGT CCTGAATGTC 
AGTCGCACCA GCATTAGTCT TCGTTGGGCT AAAAGCCAAG AGAAACCCGG AGCTGTGGGC 
CCAATCATTG GATACACTGT AGAGTACTTC AGTCCGGATC TGCAAACTGG TTGGATTGTG 
GCTGCCCAAC GAGTTGGCGA CACTCAAGTC ACTATCTCGG GTCTCACTCC TGGCACTTCG 
TATGTGTTCC TAGTTAGAGC TGAGAATACT CAGGGTATTT CTGTGCCTTC CGGCTTATCA 
AATACTATTA AAACCATTGA GGCGGATTTC GATGCAGCTT CTGCCAATGA TTTGTCAGCA 
GCTCGAACTT TGCTGACGGG AAAGTCGGTG GAGCTAATAG ATGCCTCGGC TATCAATGCT 
AGTGCCGTAA GACTTGAGTG GATGCTCCAC GTGAGCGCTG ATGAGAAATA TGTAGAGGGC 
CTGCGCATAC ACTATAAGGA TGCCAGTGTA CCATCCGCAC AGTATCACTC GATCACTGTT 
ATGGATGCCT CTGCAGAATC GTTTGTGGTG GGAAACCTTA AGAAGTACAC CAAGTATGAG 
TTCTTCCTAA CACCCTTTTT TGAGACAATT GAAGGACAGC CCAGTAACTC CAAGACAGCC 
CTCACCTATG AAGATGTTCC CTCCGCACCA CCGGATAACA TTCAGATTGG CATGTACAAC 
CAAACAGCCG GTTGGGTGCG TTGGACTCCG CCACCCTCCC AGCACCACAA TGGCAATTTG 
TATGGCTACA AGATTGAGGT CAGCGCCGGT AACACCATGA AGGTGCTGGC CAATATGACT 
CTTAATGCTA CCACCACATC TGTGCTCCTA AATAACCTAA CCACCGGAGC TGTGTACAGC 
GTGAGGTTGA ACTCCTTTAC CAAGGCAGGA GATGGACCTT ACTCCAAACC GATATCACTA 
TTCATGGACC CCACCCATCA TGTGCATCCG CCACGGGCAC ATCCAAGCGG CACCCATGAT 
GGGCGACATG AGGGACAGGA TCTCACGTAT CATAACAATG GCAACATACC ACCTGGCGAC 
ATTAATCCCA CCACTCATAA AAAGACCACT GACTACCTAT CTGGACCATG GCTAATGGTG 
CTGGTCTGCA TCGTTCTTCT AGTCCTGGTT ATTTCGGCGG CTATTTCGAT GGTCTACTTC 
AAGCGCAAGC ATCAAATGAC CAAGGAATTG GGTCACTTAA GTGTGGTCAG TGACAACGAA 
ATAACCGCAT TGAACATCAA TAGCAAAGAG AGCCTTTGGA TAGACCATCA TCGTGGATGG 
CGAACTGCCG ATACTGACAA AGACTCAGGA TTAAGCGAAT CGAAGCTACT ATCCCACGTA 
AACAGCAGCC AATCCAACTA CAATAACTCC GATGGAGGAA CCGATTATGC AGAAGTTGAC 
ACCCGTAACC TTACCACCTT CTACAATTGT CGCAAGAGCC CCGATAATCC CACGCCGTAC 
GCCACCACTA TGATCATTGG TACCTCTTCC AGTGAGACCT GCACCAAGAC AACATCTATA 
AGTGCCGATA AGGACTCGGG AACTCATTCG CCCTATTCTG ACGCATTTGC CGGTCAGGTG 
CCAGCGGTTC CTGTTGTCAA ATCCAACTAT CTTCAGTATC CGGTTGAACC GATCAACTGG 
TCAGAGTTTC TACCCCCGCC GCCAGAACAC CCACCTCCGT CTTCTACCTA TGGATACGCA 
CAAGGATCTC CTGAATCTTC ACGGAAGAGC TCCAAAAGCG CAGGTTCCGG CATTTCTACA 
AATCAAAGCA TTCTGAACGC CTCCATACAC AGCAGCTCCT CGGGCGGCTT TTCAGCTTGG 
GGAGTTTCGC CCCAATATGC TGTCGCCTGT CCACCGGAAA ACGTTTATAG CAATCCGCTG 
TCGGCAGTGG CTGGCGGCAC CCAGAACCGC TATCAGATAA CGCCAACAAA CCAACATCCG 
CCACAGTTAC CGGCCTACTT TGCCACCACG GGTCCAGGAG GAGCTGTACC ACCCAACCAC 
CTGCCATTTG CCACACAGCG TCATGCAGCC AGCGAGTACC AGGCTGGACT GAATGCAGCG 
CGATGTGCCC AAAGCCGCGC CTGCAACAGC TGCGATGCCT TGGCCACACC CTCGCCCATG 
CAACCCCCAC CGCCAGTTCC CGTACCCGAG GGCTGGTACC AACCGGTGCA TCCCAATAGC 
CACCCGATGC ACCCGACCTC CTCCAACCAC CAGATCTACC AGTGCTCCTC CGAGTGCTCG 
GATCACTCGA GGAGCTCGCA GAGTCACAAG CGGCAGCTGC AGCTCGAGGA GCACGGCAGC 
AGTGCCAAAC AACGCGGAGG ACACCACCGT CGACGAGCCC CGGTGGTGCA GCCGTGCATG 
GAGAGCGAGA ACGAGAACAT GCTGGCGGAG TACGAGCAGC GCCAGTACAC CAGCGATTGT 
TGCAATAGCT CCCGCGAGGG CGACACCTGC TCCTGCAGCG AGGGATCCTG TCTTTACGCC 
GAGGCGGGCG AGCCGGCGCC TCGTCAAATG ACTGCTAAGA ACACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726224.3
CDS	482..4669
Translation	MHPMHPENHAIARSTSTTNNPSRSRSSRMWLLPAWLLLVLVASNGLPAVRGQYQSPRIIEHPTDLVVKKNEPATLNCKVEGKPEPTIEWFKDGEPVSTNEKKSHRVQFKDGALFFYRTMQGKKEQDGGEYWCVAKNRVGQAVSRHASLQIAVLRDDFRVEPKDTRVAKGETALLECGPPKGIPEPTLIWIKDGVPLDDLKAMSFGASSRVRIVDGGNLLISNVEPIDEGNYKCIAQNLVGTRESSYAKLIVQVKPYFMKEPKDQVMLYGQTATFHCSVGGDPPPKVLWKKEEGNIPVSRARILHDEKSLEISNITPTDEGTYVCEAHNNVGQISARASLIVHAPPNFTKRPSNKKVGLNGVVQLPCMASGNPPPSVFWTKEGVSTLMFPNSSHGRQHVAADGTLQITDVRQEDEGYYVCSAFSVVDSSTVRVFLQVSSLDERPPPIIQIGPANQTLPKGSVATLPCRATGNPSPRIKWFHDGHAVQAGNRYSIIQGSSLRVDDLQLSDSGTYTCTASGERGETSWAATLTVEKPGSTSLHRAADPSTYPAPPGTPKVLNVSRTSISLRWAKSQEKPGAVGPIIGYTVEYFSPDLQTGWIVAAQRVGDTQVTISGLTPGTSYVFLVRAENTQGISVPSGLSNTIKTIEADFDAASANDLSAARTLLTGKSVELIDASAINASAVRLEWMLHVSADEKYVEGLRIHYKDASVPSAQYHSITVMDASAESFVVGNLKKYTKYEFFLTPFFETIEGQPSNSKTALTYEDVPSAPPDNIQIGMYNQTAGWVRWTPPPSQHHNGNLYGYKIEVSAGNTMKVLANMTLNATTTSVLLNNLTTGAVYSVRLNSFTKAGDGPYSKPISLFMDPTHHVHPPRAHPSGTHDGRHEGQDLTYHNNGNIPPGDINPTTHKKTTDYLSGPWLMVLVCIVLLVLVISAAISMVYFKRKHQMTKELGHLSVVSDNEITALNINSKESLWIDHHRGWRTADTDKDSGLSESKLLSHVNSSQSNYNNSDGGTDYAEVDTRNLTTFYNCRKSPDNPTPYATTMIIGTSSSETCTKTTSISADKDSGTHSPYSDAFAGQVPAVPVVKSNYLQYPVEPINWSEFLPPPPEHPPPSSTYGYAQGSPESSRKSSKSAGSGISTNQSILNASIHSSSSGGFSAWGVSPQYAVACPPENVYSNPLSAVAGGTQNRYQITPTNQHPPQLPAYFATTGPGGAVPPNHLPFATQRHAASEYQAGLNAARCAQSRACNSCDALATPSPMQPPPPVPVPEGWYQPVHPNSHPMHPTSSNHQIYQCSSECSDHSRSSQSHKRQLQLEEHGSSAKQRGGHHRRRAPVVQPCMESENENMLAEYEQRQYTSDCCNSSREGDTCSCSEGSCLYAEAGEPAPRQMTAKNT

Target ORF information:

RefSeq Version	NM_166543.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster roundabout 1 (robo1), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166543.2

ORF Insert Sequence:

ATGCATCCCA TGCATCCCGA AAACCACGCC ATCGCCCGGA GCACGAGCAC CACTAATAAC 
CCATCTCGCA GTCGGAGCAG CAGGATGTGG CTCCTGCCCG CCTGGCTGCT CCTCGTCCTG 
GTGGCCAGCA ATGGCCTGCC AGCAGTCAGA GGCCAGTACC AATCGCCACG TATCATCGAG 
CATCCCACGG ATCTGGTCGT TAAGAAGAAT GAACCCGCCA CGCTCAACTG CAAAGTGGAG 
GGCAAGCCGG AACCCACCAT TGAGTGGTTT AAGGATGGCG AACCCGTCAG CACCAACGAA 
AAGAAATCGC ACCGCGTCCA GTTCAAGGAC GGCGCCCTCT TCTTCTACAG GACAATGCAG 
GGCAAGAAGG AACAGGACGG CGGAGAGTAC TGGTGCGTGG CCAAGAACCG AGTGGGCCAG 
GCCGTTAGTC GCCATGCCTC GCTCCAGATA GCTGTTTTGC GCGACGATTT TCGCGTGGAG 
CCCAAAGACA CGCGAGTGGC CAAAGGCGAG ACGGCTCTGC TGGAGTGTGG GCCGCCCAAA 
GGCATTCCAG AGCCAACGCT GATTTGGATA AAGGACGGCG TTCCCTTGGA CGACCTGAAA 
GCCATGTCGT TTGGCGCCAG CTCCCGCGTT CGAATTGTGG ACGGTGGCAA CCTGCTGATC 
AGCAATGTGG AGCCCATTGA TGAGGGCAAC TACAAGTGCA TTGCCCAGAA TCTGGTAGGC 
ACCCGCGAGA GCAGCTATGC CAAGCTGATT GTCCAGGTCA AACCATACTT TATGAAGGAG 
CCCAAGGATC AGGTGATGCT CTACGGCCAG ACAGCCACTT TCCACTGCTC AGTGGGCGGT 
GATCCGCCGC CGAAAGTGTT GTGGAAAAAG GAGGAGGGCA ATATTCCGGT GTCCAGAGCG 
CGAATCCTTC ACGACGAGAA AAGTTTAGAG ATATCCAACA TAACGCCCAC CGATGAGGGC 
ACCTATGTCT GCGAGGCACA CAACAATGTC GGTCAGATCA GCGCTAGGGC TTCTCTTATA 
GTCCATGCTC CGCCGAACTT TACGAAAAGA CCCAGTAACA AGAAAGTGGG ACTAAATGGT 
GTTGTCCAAC TACCTTGCAT GGCCTCCGGA AACCCACCGC CATCTGTTTT CTGGACCAAG 
GAAGGAGTAT CCACTCTCAT GTTCCCAAAT AGTTCGCACG GGAGGCAGCA TGTGGCTGCC 
GATGGAACTC TGCAGATTAC GGATGTGCGG CAGGAAGACG AAGGCTACTA TGTGTGTTCC 
GCTTTCAGTG TAGTCGATTC CTCTACAGTA CGGGTTTTCC TGCAAGTCAG CTCGTTAGAC 
GAGCGTCCAC CTCCGATTAT TCAAATCGGA CCTGCCAATC AAACACTGCC CAAGGGATCA 
GTTGCTACTT TACCCTGTCG GGCCACCGGA AATCCCAGTC CCCGTATTAA GTGGTTCCAC 
GATGGACATG CCGTACAAGC GGGCAATCGA TACAGCATCA TCCAAGGAAG CTCACTGAGA 
GTCGATGACC TTCAACTTAG TGACTCTGGT ACCTACACCT GCACTGCATC TGGCGAACGA 
GGAGAAACTT CCTGGGCTGC CACACTAACG GTGGAAAAAC CCGGTTCTAC ATCTCTTCAC 
CGGGCAGCTG ATCCTAGCAC TTATCCTGCT CCTCCAGGAA CACCTAAAGT CCTGAATGTC 
AGTCGCACCA GCATTAGTCT TCGTTGGGCT AAAAGCCAAG AGAAACCCGG AGCTGTGGGC 
CCAATCATTG GATACACTGT AGAGTACTTC AGTCCGGATC TGCAAACTGG TTGGATTGTG 
GCTGCCCAAC GAGTTGGCGA CACTCAAGTC ACTATCTCGG GTCTCACTCC TGGCACTTCG 
TATGTGTTCC TAGTTAGAGC TGAGAATACT CAGGGTATTT CTGTGCCTTC CGGCTTATCA 
AATACTATTA AAACCATTGA GGCGGATTTC GATGCAGCTT CTGCCAATGA TTTGTCAGCA 
GCTCGAACTT TGCTGACGGG AAAGTCGGTG GAGCTAATAG ATGCCTCGGC TATCAATGCT 
AGTGCCGTAA GACTTGAGTG GATGCTCCAC GTGAGCGCTG ATGAGAAATA TGTAGAGGGC 
CTGCGCATAC ACTATAAGGA TGCCAGTGTA CCATCCGCAC AGTATCACTC GATCACTGTT 
ATGGATGCCT CTGCAGAATC GTTTGTGGTG GGAAACCTTA AGAAGTACAC CAAGTATGAG 
TTCTTCCTAA CACCCTTTTT TGAGACAATT GAAGGACAGC CCAGTAACTC CAAGACAGCC 
CTCACCTATG AAGATGTTCC CTCCGCACCA CCGGATAACA TTCAGATTGG CATGTACAAC 
CAAACAGCCG GTTGGGTGCG TTGGACTCCG CCACCCTCCC AGCACCACAA TGGCAATTTG 
TATGGCTACA AGATTGAGGT CAGCGCCGGT AACACCATGA AGGTGCTGGC CAATATGACT 
CTTAATGCTA CCACCACATC TGTGCTCCTA AATAACCTAA CCACCGGAGC TGTGTACAGC 
GTGAGGTTGA ACTCCTTTAC CAAGGCAGGA GATGGACCTT ACTCCAAACC GATATCACTA 
TTCATGGACC CCACCCATCA TGTGCATCCG CCACGGGCAC ATCCAAGCGG CACCCATGAT 
GGGCGACATG AGGGACAGGA TCTCACGTAT CATAACAATG GCAACATACC ACCTGGCGAC 
ATTAATCCCA CCACTCATAA AAAGACCACT GACTACCTAT CTGGACCATG GCTAATGGTG 
CTGGTCTGCA TCGTTCTTCT AGTCCTGGTT ATTTCGGCGG CTATTTCGAT GGTCTACTTC 
AAGCGCAAGC ATCAAATGAC CAAGGAATTG GGTCACTTAA GTGTGGTCAG TGACAACGAA 
ATAACCGCAT TGAACATCAA TAGCAAAGAG AGCCTTTGGA TAGACCATCA TCGTGGATGG 
CGAACTGCCG ATACTGACAA AGACTCAGGA TTAAGCGAAT CGAAGCTACT ATCCCACGTA 
AACAGCAGCC AATCCAACTA CAATAACTCC GATGGAGGAA CCGATTATGC AGAAGTTGAC 
ACCCGTAACC TTACCACCTT CTACAATTGT CGCAAGAGCC CCGATAATCC CACGCCGTAC 
GCCACCACTA TGATCATTGG TACCTCTTCC AGTGAGACCT GCACCAAGAC AACATCTATA 
AGTGCCGATA AGGACTCGGG AACTCATTCG CCCTATTCTG ACGCATTTGC CGGTCAGGTG 
CCAGCGGTTC CTGTTGTCAA ATCCAACTAT CTTCAGTATC CGGTTGAACC GATCAACTGG 
TCAGAGTTTC TACCCCCGCC GCCAGAACAC CCACCTCCGT CTTCTACCTA TGGATACGCA 
CAAGGATCTC CTGAATCTTC ACGGAAGAGC TCCAAAAGCG CAGGTTCCGG CATTTCTACA 
AATCAAAGCA TTCTGAACGC CTCCATACAC AGCAGCTCCT CGGGCGGCTT TTCAGCTTGG 
GGAGTTTCGC CCCAATATGC TGTCGCCTGT CCACCGGAAA ACGTTTATAG CAATCCGCTG 
TCGGCAGTGG CTGGCGGCAC CCAGAACCGC TATCAGATAA CGCCAACAAA CCAACATCCG 
CCACAGTTAC CGGCCTACTT TGCCACCACG GGTCCAGGAG GAGCTGTACC ACCCAACCAC 
CTGCCATTTG CCACACAGCG TCATGCAGCC AGCGAGTACC AGGCTGGACT GAATGCAGCG 
CGATGTGCCC AAAGCCGCGC CTGCAACAGC TGCGATGCCT TGGCCACACC CTCGCCCATG 
CAACCCCCAC CGCCAGTTCC CGTACCCGAG GGCTGGTACC AACCGGTGCA TCCCAATAGC 
CACCCGATGC ACCCGACCTC CTCCAACCAC CAGATCTACC AGTGCTCCTC CGAGTGCTCG 
GATCACTCGA GGAGCTCGCA GAGTCACAAG CGGCAGCTGC AGCTCGAGGA GCACGGCAGC 
AGTGCCAAAC AACGCGGAGG ACACCACCGT CGACGAGCCC CGGTGGTGCA GCCGTGCATG 
GAGAGCGAGA ACGAGAACAT GCTGGCGGAG TACGAGCAGC GCCAGTACAC CAGCGATTGT 
TGCAATAGCT CCCGCGAGGG CGACACCTGC TCCTGCAGCG AGGGATCCTG TCTTTACGCC 
GAGGCGGGCG AGCCGGCGCC TCGTCAAATG ACTGCTAAGA ACACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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robo1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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