robo1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following robo1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the robo1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa12568 NM_166543.2
Latest version!
Drosophila melanogaster roundabout 1 (robo1), transcript variant C, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OFa12568 NM_057551.4
Latest version!
Drosophila melanogaster roundabout 1 (robo1), transcript variant A, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa12568
    Clone ID Related Accession (Same CDS sequence) NM_057551.4 , NM_166543.2
    Accession Version NM_057551.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4188bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product roundabout 1, isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_057551.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCATCCCA TGCATCCCGA AAACCACGCC ATCGCCCGGA GCACGAGCAC CACTAATAAC 
    CCATCTCGCA GTCGGAGCAG CAGGATGTGG CTCCTGCCCG CCTGGCTGCT CCTCGTCCTG
    GTGGCCAGCA ATGGCCTGCC AGCAGTCAGA GGCCAGTACC AATCGCCACG TATCATCGAG
    CATCCCACGG ATCTGGTCGT TAAGAAGAAT GAACCCGCCA CGCTCAACTG CAAAGTGGAG
    GGCAAGCCGG AACCCACCAT TGAGTGGTTT AAGGATGGCG AACCCGTCAG CACCAACGAA
    AAGAAATCGC ACCGCGTCCA GTTCAAGGAC GGCGCCCTCT TCTTCTACAG GACAATGCAG
    GGCAAGAAGG AACAGGACGG CGGAGAGTAC TGGTGCGTGG CCAAGAACCG AGTGGGCCAG
    GCCGTTAGTC GCCATGCCTC GCTCCAGATA GCTGTTTTGC GCGACGATTT TCGCGTGGAG
    CCCAAAGACA CGCGAGTGGC CAAAGGCGAG ACGGCTCTGC TGGAGTGTGG GCCGCCCAAA
    GGCATTCCAG AGCCAACGCT GATTTGGATA AAGGACGGCG TTCCCTTGGA CGACCTGAAA
    GCCATGTCGT TTGGCGCCAG CTCCCGCGTT CGAATTGTGG ACGGTGGCAA CCTGCTGATC
    AGCAATGTGG AGCCCATTGA TGAGGGCAAC TACAAGTGCA TTGCCCAGAA TCTGGTAGGC
    ACCCGCGAGA GCAGCTATGC CAAGCTGATT GTCCAGGTCA AACCATACTT TATGAAGGAG
    CCCAAGGATC AGGTGATGCT CTACGGCCAG ACAGCCACTT TCCACTGCTC AGTGGGCGGT
    GATCCGCCGC CGAAAGTGTT GTGGAAAAAG GAGGAGGGCA ATATTCCGGT GTCCAGAGCG
    CGAATCCTTC ACGACGAGAA AAGTTTAGAG ATATCCAACA TAACGCCCAC CGATGAGGGC
    ACCTATGTCT GCGAGGCACA CAACAATGTC GGTCAGATCA GCGCTAGGGC TTCTCTTATA
    GTCCATGCTC CGCCGAACTT TACGAAAAGA CCCAGTAACA AGAAAGTGGG ACTAAATGGT
    GTTGTCCAAC TACCTTGCAT GGCCTCCGGA AACCCACCGC CATCTGTTTT CTGGACCAAG
    GAAGGAGTAT CCACTCTCAT GTTCCCAAAT AGTTCGCACG GGAGGCAGCA TGTGGCTGCC
    GATGGAACTC TGCAGATTAC GGATGTGCGG CAGGAAGACG AAGGCTACTA TGTGTGTTCC
    GCTTTCAGTG TAGTCGATTC CTCTACAGTA CGGGTTTTCC TGCAAGTCAG CTCGTTAGAC
    GAGCGTCCAC CTCCGATTAT TCAAATCGGA CCTGCCAATC AAACACTGCC CAAGGGATCA
    GTTGCTACTT TACCCTGTCG GGCCACCGGA AATCCCAGTC CCCGTATTAA GTGGTTCCAC
    GATGGACATG CCGTACAAGC GGGCAATCGA TACAGCATCA TCCAAGGAAG CTCACTGAGA
    GTCGATGACC TTCAACTTAG TGACTCTGGT ACCTACACCT GCACTGCATC TGGCGAACGA
    GGAGAAACTT CCTGGGCTGC CACACTAACG GTGGAAAAAC CCGGTTCTAC ATCTCTTCAC
    CGGGCAGCTG ATCCTAGCAC TTATCCTGCT CCTCCAGGAA CACCTAAAGT CCTGAATGTC
    AGTCGCACCA GCATTAGTCT TCGTTGGGCT AAAAGCCAAG AGAAACCCGG AGCTGTGGGC
    CCAATCATTG GATACACTGT AGAGTACTTC AGTCCGGATC TGCAAACTGG TTGGATTGTG
    GCTGCCCAAC GAGTTGGCGA CACTCAAGTC ACTATCTCGG GTCTCACTCC TGGCACTTCG
    TATGTGTTCC TAGTTAGAGC TGAGAATACT CAGGGTATTT CTGTGCCTTC CGGCTTATCA
    AATACTATTA AAACCATTGA GGCGGATTTC GATGCAGCTT CTGCCAATGA TTTGTCAGCA
    GCTCGAACTT TGCTGACGGG AAAGTCGGTG GAGCTAATAG ATGCCTCGGC TATCAATGCT
    AGTGCCGTAA GACTTGAGTG GATGCTCCAC GTGAGCGCTG ATGAGAAATA TGTAGAGGGC
    CTGCGCATAC ACTATAAGGA TGCCAGTGTA CCATCCGCAC AGTATCACTC GATCACTGTT
    ATGGATGCCT CTGCAGAATC GTTTGTGGTG GGAAACCTTA AGAAGTACAC CAAGTATGAG
    TTCTTCCTAA CACCCTTTTT TGAGACAATT GAAGGACAGC CCAGTAACTC CAAGACAGCC
    CTCACCTATG AAGATGTTCC CTCCGCACCA CCGGATAACA TTCAGATTGG CATGTACAAC
    CAAACAGCCG GTTGGGTGCG TTGGACTCCG CCACCCTCCC AGCACCACAA TGGCAATTTG
    TATGGCTACA AGATTGAGGT CAGCGCCGGT AACACCATGA AGGTGCTGGC CAATATGACT
    CTTAATGCTA CCACCACATC TGTGCTCCTA AATAACCTAA CCACCGGAGC TGTGTACAGC
    GTGAGGTTGA ACTCCTTTAC CAAGGCAGGA GATGGACCTT ACTCCAAACC GATATCACTA
    TTCATGGACC CCACCCATCA TGTGCATCCG CCACGGGCAC ATCCAAGCGG CACCCATGAT
    GGGCGACATG AGGGACAGGA TCTCACGTAT CATAACAATG GCAACATACC ACCTGGCGAC
    ATTAATCCCA CCACTCATAA AAAGACCACT GACTACCTAT CTGGACCATG GCTAATGGTG
    CTGGTCTGCA TCGTTCTTCT AGTCCTGGTT ATTTCGGCGG CTATTTCGAT GGTCTACTTC
    AAGCGCAAGC ATCAAATGAC CAAGGAATTG GGTCACTTAA GTGTGGTCAG TGACAACGAA
    ATAACCGCAT TGAACATCAA TAGCAAAGAG AGCCTTTGGA TAGACCATCA TCGTGGATGG
    CGAACTGCCG ATACTGACAA AGACTCAGGA TTAAGCGAAT CGAAGCTACT ATCCCACGTA
    AACAGCAGCC AATCCAACTA CAATAACTCC GATGGAGGAA CCGATTATGC AGAAGTTGAC
    ACCCGTAACC TTACCACCTT CTACAATTGT CGCAAGAGCC CCGATAATCC CACGCCGTAC
    GCCACCACTA TGATCATTGG TACCTCTTCC AGTGAGACCT GCACCAAGAC AACATCTATA
    AGTGCCGATA AGGACTCGGG AACTCATTCG CCCTATTCTG ACGCATTTGC CGGTCAGGTG
    CCAGCGGTTC CTGTTGTCAA ATCCAACTAT CTTCAGTATC CGGTTGAACC GATCAACTGG
    TCAGAGTTTC TACCCCCGCC GCCAGAACAC CCACCTCCGT CTTCTACCTA TGGATACGCA
    CAAGGATCTC CTGAATCTTC ACGGAAGAGC TCCAAAAGCG CAGGTTCCGG CATTTCTACA
    AATCAAAGCA TTCTGAACGC CTCCATACAC AGCAGCTCCT CGGGCGGCTT TTCAGCTTGG
    GGAGTTTCGC CCCAATATGC TGTCGCCTGT CCACCGGAAA ACGTTTATAG CAATCCGCTG
    TCGGCAGTGG CTGGCGGCAC CCAGAACCGC TATCAGATAA CGCCAACAAA CCAACATCCG
    CCACAGTTAC CGGCCTACTT TGCCACCACG GGTCCAGGAG GAGCTGTACC ACCCAACCAC
    CTGCCATTTG CCACACAGCG TCATGCAGCC AGCGAGTACC AGGCTGGACT GAATGCAGCG
    CGATGTGCCC AAAGCCGCGC CTGCAACAGC TGCGATGCCT TGGCCACACC CTCGCCCATG
    CAACCCCCAC CGCCAGTTCC CGTACCCGAG GGCTGGTACC AACCGGTGCA TCCCAATAGC
    CACCCGATGC ACCCGACCTC CTCCAACCAC CAGATCTACC AGTGCTCCTC CGAGTGCTCG
    GATCACTCGA GGAGCTCGCA GAGTCACAAG CGGCAGCTGC AGCTCGAGGA GCACGGCAGC
    AGTGCCAAAC AACGCGGAGG ACACCACCGT CGACGAGCCC CGGTGGTGCA GCCGTGCATG
    GAGAGCGAGA ACGAGAACAT GCTGGCGGAG TACGAGCAGC GCCAGTACAC CAGCGATTGT
    TGCAATAGCT CCCGCGAGGG CGACACCTGC TCCTGCAGCG AGGGATCCTG TCTTTACGCC
    GAGGCGGGCG AGCCGGCGCC TCGTCAAATG ACTGCTAAGA ACACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_476899.1
    CDS176..4363
    Translation

    Target ORF information:

    RefSeq Version NM_057551.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster roundabout 1 (robo1), transcript variant A, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_057551.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCATCCCA TGCATCCCGA AAACCACGCC ATCGCCCGGA GCACGAGCAC CACTAATAAC 
    CCATCTCGCA GTCGGAGCAG CAGGATGTGG CTCCTGCCCG CCTGGCTGCT CCTCGTCCTG
    GTGGCCAGCA ATGGCCTGCC AGCAGTCAGA GGCCAGTACC AATCGCCACG TATCATCGAG
    CATCCCACGG ATCTGGTCGT TAAGAAGAAT GAACCCGCCA CGCTCAACTG CAAAGTGGAG
    GGCAAGCCGG AACCCACCAT TGAGTGGTTT AAGGATGGCG AACCCGTCAG CACCAACGAA
    AAGAAATCGC ACCGCGTCCA GTTCAAGGAC GGCGCCCTCT TCTTCTACAG GACAATGCAG
    GGCAAGAAGG AACAGGACGG CGGAGAGTAC TGGTGCGTGG CCAAGAACCG AGTGGGCCAG
    GCCGTTAGTC GCCATGCCTC GCTCCAGATA GCTGTTTTGC GCGACGATTT TCGCGTGGAG
    CCCAAAGACA CGCGAGTGGC CAAAGGCGAG ACGGCTCTGC TGGAGTGTGG GCCGCCCAAA
    GGCATTCCAG AGCCAACGCT GATTTGGATA AAGGACGGCG TTCCCTTGGA CGACCTGAAA
    GCCATGTCGT TTGGCGCCAG CTCCCGCGTT CGAATTGTGG ACGGTGGCAA CCTGCTGATC
    AGCAATGTGG AGCCCATTGA TGAGGGCAAC TACAAGTGCA TTGCCCAGAA TCTGGTAGGC
    ACCCGCGAGA GCAGCTATGC CAAGCTGATT GTCCAGGTCA AACCATACTT TATGAAGGAG
    CCCAAGGATC AGGTGATGCT CTACGGCCAG ACAGCCACTT TCCACTGCTC AGTGGGCGGT
    GATCCGCCGC CGAAAGTGTT GTGGAAAAAG GAGGAGGGCA ATATTCCGGT GTCCAGAGCG
    CGAATCCTTC ACGACGAGAA AAGTTTAGAG ATATCCAACA TAACGCCCAC CGATGAGGGC
    ACCTATGTCT GCGAGGCACA CAACAATGTC GGTCAGATCA GCGCTAGGGC TTCTCTTATA
    GTCCATGCTC CGCCGAACTT TACGAAAAGA CCCAGTAACA AGAAAGTGGG ACTAAATGGT
    GTTGTCCAAC TACCTTGCAT GGCCTCCGGA AACCCACCGC CATCTGTTTT CTGGACCAAG
    GAAGGAGTAT CCACTCTCAT GTTCCCAAAT AGTTCGCACG GGAGGCAGCA TGTGGCTGCC
    GATGGAACTC TGCAGATTAC GGATGTGCGG CAGGAAGACG AAGGCTACTA TGTGTGTTCC
    GCTTTCAGTG TAGTCGATTC CTCTACAGTA CGGGTTTTCC TGCAAGTCAG CTCGTTAGAC
    GAGCGTCCAC CTCCGATTAT TCAAATCGGA CCTGCCAATC AAACACTGCC CAAGGGATCA
    GTTGCTACTT TACCCTGTCG GGCCACCGGA AATCCCAGTC CCCGTATTAA GTGGTTCCAC
    GATGGACATG CCGTACAAGC GGGCAATCGA TACAGCATCA TCCAAGGAAG CTCACTGAGA
    GTCGATGACC TTCAACTTAG TGACTCTGGT ACCTACACCT GCACTGCATC TGGCGAACGA
    GGAGAAACTT CCTGGGCTGC CACACTAACG GTGGAAAAAC CCGGTTCTAC ATCTCTTCAC
    CGGGCAGCTG ATCCTAGCAC TTATCCTGCT CCTCCAGGAA CACCTAAAGT CCTGAATGTC
    AGTCGCACCA GCATTAGTCT TCGTTGGGCT AAAAGCCAAG AGAAACCCGG AGCTGTGGGC
    CCAATCATTG GATACACTGT AGAGTACTTC AGTCCGGATC TGCAAACTGG TTGGATTGTG
    GCTGCCCAAC GAGTTGGCGA CACTCAAGTC ACTATCTCGG GTCTCACTCC TGGCACTTCG
    TATGTGTTCC TAGTTAGAGC TGAGAATACT CAGGGTATTT CTGTGCCTTC CGGCTTATCA
    AATACTATTA AAACCATTGA GGCGGATTTC GATGCAGCTT CTGCCAATGA TTTGTCAGCA
    GCTCGAACTT TGCTGACGGG AAAGTCGGTG GAGCTAATAG ATGCCTCGGC TATCAATGCT
    AGTGCCGTAA GACTTGAGTG GATGCTCCAC GTGAGCGCTG ATGAGAAATA TGTAGAGGGC
    CTGCGCATAC ACTATAAGGA TGCCAGTGTA CCATCCGCAC AGTATCACTC GATCACTGTT
    ATGGATGCCT CTGCAGAATC GTTTGTGGTG GGAAACCTTA AGAAGTACAC CAAGTATGAG
    TTCTTCCTAA CACCCTTTTT TGAGACAATT GAAGGACAGC CCAGTAACTC CAAGACAGCC
    CTCACCTATG AAGATGTTCC CTCCGCACCA CCGGATAACA TTCAGATTGG CATGTACAAC
    CAAACAGCCG GTTGGGTGCG TTGGACTCCG CCACCCTCCC AGCACCACAA TGGCAATTTG
    TATGGCTACA AGATTGAGGT CAGCGCCGGT AACACCATGA AGGTGCTGGC CAATATGACT
    CTTAATGCTA CCACCACATC TGTGCTCCTA AATAACCTAA CCACCGGAGC TGTGTACAGC
    GTGAGGTTGA ACTCCTTTAC CAAGGCAGGA GATGGACCTT ACTCCAAACC GATATCACTA
    TTCATGGACC CCACCCATCA TGTGCATCCG CCACGGGCAC ATCCAAGCGG CACCCATGAT
    GGGCGACATG AGGGACAGGA TCTCACGTAT CATAACAATG GCAACATACC ACCTGGCGAC
    ATTAATCCCA CCACTCATAA AAAGACCACT GACTACCTAT CTGGACCATG GCTAATGGTG
    CTGGTCTGCA TCGTTCTTCT AGTCCTGGTT ATTTCGGCGG CTATTTCGAT GGTCTACTTC
    AAGCGCAAGC ATCAAATGAC CAAGGAATTG GGTCACTTAA GTGTGGTCAG TGACAACGAA
    ATAACCGCAT TGAACATCAA TAGCAAAGAG AGCCTTTGGA TAGACCATCA TCGTGGATGG
    CGAACTGCCG ATACTGACAA AGACTCAGGA TTAAGCGAAT CGAAGCTACT ATCCCACGTA
    AACAGCAGCC AATCCAACTA CAATAACTCC GATGGAGGAA CCGATTATGC AGAAGTTGAC
    ACCCGTAACC TTACCACCTT CTACAATTGT CGCAAGAGCC CCGATAATCC CACGCCGTAC
    GCCACCACTA TGATCATTGG TACCTCTTCC AGTGAGACCT GCACCAAGAC AACATCTATA
    AGTGCCGATA AGGACTCGGG AACTCATTCG CCCTATTCTG ACGCATTTGC CGGTCAGGTG
    CCAGCGGTTC CTGTTGTCAA ATCCAACTAT CTTCAGTATC CGGTTGAACC GATCAACTGG
    TCAGAGTTTC TACCCCCGCC GCCAGAACAC CCACCTCCGT CTTCTACCTA TGGATACGCA
    CAAGGATCTC CTGAATCTTC ACGGAAGAGC TCCAAAAGCG CAGGTTCCGG CATTTCTACA
    AATCAAAGCA TTCTGAACGC CTCCATACAC AGCAGCTCCT CGGGCGGCTT TTCAGCTTGG
    GGAGTTTCGC CCCAATATGC TGTCGCCTGT CCACCGGAAA ACGTTTATAG CAATCCGCTG
    TCGGCAGTGG CTGGCGGCAC CCAGAACCGC TATCAGATAA CGCCAACAAA CCAACATCCG
    CCACAGTTAC CGGCCTACTT TGCCACCACG GGTCCAGGAG GAGCTGTACC ACCCAACCAC
    CTGCCATTTG CCACACAGCG TCATGCAGCC AGCGAGTACC AGGCTGGACT GAATGCAGCG
    CGATGTGCCC AAAGCCGCGC CTGCAACAGC TGCGATGCCT TGGCCACACC CTCGCCCATG
    CAACCCCCAC CGCCAGTTCC CGTACCCGAG GGCTGGTACC AACCGGTGCA TCCCAATAGC
    CACCCGATGC ACCCGACCTC CTCCAACCAC CAGATCTACC AGTGCTCCTC CGAGTGCTCG
    GATCACTCGA GGAGCTCGCA GAGTCACAAG CGGCAGCTGC AGCTCGAGGA GCACGGCAGC
    AGTGCCAAAC AACGCGGAGG ACACCACCGT CGACGAGCCC CGGTGGTGCA GCCGTGCATG
    GAGAGCGAGA ACGAGAACAT GCTGGCGGAG TACGAGCAGC GCCAGTACAC CAGCGATTGT
    TGCAATAGCT CCCGCGAGGG CGACACCTGC TCCTGCAGCG AGGGATCCTG TCTTTACGCC
    GAGGCGGGCG AGCCGGCGCC TCGTCAAATG ACTGCTAAGA ACACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa12568
    Clone ID Related Accession (Same CDS sequence) NM_057551.4 , NM_166543.2
    Accession Version NM_166543.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4188bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product roundabout 1, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166543.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCATCCCA TGCATCCCGA AAACCACGCC ATCGCCCGGA GCACGAGCAC CACTAATAAC 
    CCATCTCGCA GTCGGAGCAG CAGGATGTGG CTCCTGCCCG CCTGGCTGCT CCTCGTCCTG
    GTGGCCAGCA ATGGCCTGCC AGCAGTCAGA GGCCAGTACC AATCGCCACG TATCATCGAG
    CATCCCACGG ATCTGGTCGT TAAGAAGAAT GAACCCGCCA CGCTCAACTG CAAAGTGGAG
    GGCAAGCCGG AACCCACCAT TGAGTGGTTT AAGGATGGCG AACCCGTCAG CACCAACGAA
    AAGAAATCGC ACCGCGTCCA GTTCAAGGAC GGCGCCCTCT TCTTCTACAG GACAATGCAG
    GGCAAGAAGG AACAGGACGG CGGAGAGTAC TGGTGCGTGG CCAAGAACCG AGTGGGCCAG
    GCCGTTAGTC GCCATGCCTC GCTCCAGATA GCTGTTTTGC GCGACGATTT TCGCGTGGAG
    CCCAAAGACA CGCGAGTGGC CAAAGGCGAG ACGGCTCTGC TGGAGTGTGG GCCGCCCAAA
    GGCATTCCAG AGCCAACGCT GATTTGGATA AAGGACGGCG TTCCCTTGGA CGACCTGAAA
    GCCATGTCGT TTGGCGCCAG CTCCCGCGTT CGAATTGTGG ACGGTGGCAA CCTGCTGATC
    AGCAATGTGG AGCCCATTGA TGAGGGCAAC TACAAGTGCA TTGCCCAGAA TCTGGTAGGC
    ACCCGCGAGA GCAGCTATGC CAAGCTGATT GTCCAGGTCA AACCATACTT TATGAAGGAG
    CCCAAGGATC AGGTGATGCT CTACGGCCAG ACAGCCACTT TCCACTGCTC AGTGGGCGGT
    GATCCGCCGC CGAAAGTGTT GTGGAAAAAG GAGGAGGGCA ATATTCCGGT GTCCAGAGCG
    CGAATCCTTC ACGACGAGAA AAGTTTAGAG ATATCCAACA TAACGCCCAC CGATGAGGGC
    ACCTATGTCT GCGAGGCACA CAACAATGTC GGTCAGATCA GCGCTAGGGC TTCTCTTATA
    GTCCATGCTC CGCCGAACTT TACGAAAAGA CCCAGTAACA AGAAAGTGGG ACTAAATGGT
    GTTGTCCAAC TACCTTGCAT GGCCTCCGGA AACCCACCGC CATCTGTTTT CTGGACCAAG
    GAAGGAGTAT CCACTCTCAT GTTCCCAAAT AGTTCGCACG GGAGGCAGCA TGTGGCTGCC
    GATGGAACTC TGCAGATTAC GGATGTGCGG CAGGAAGACG AAGGCTACTA TGTGTGTTCC
    GCTTTCAGTG TAGTCGATTC CTCTACAGTA CGGGTTTTCC TGCAAGTCAG CTCGTTAGAC
    GAGCGTCCAC CTCCGATTAT TCAAATCGGA CCTGCCAATC AAACACTGCC CAAGGGATCA
    GTTGCTACTT TACCCTGTCG GGCCACCGGA AATCCCAGTC CCCGTATTAA GTGGTTCCAC
    GATGGACATG CCGTACAAGC GGGCAATCGA TACAGCATCA TCCAAGGAAG CTCACTGAGA
    GTCGATGACC TTCAACTTAG TGACTCTGGT ACCTACACCT GCACTGCATC TGGCGAACGA
    GGAGAAACTT CCTGGGCTGC CACACTAACG GTGGAAAAAC CCGGTTCTAC ATCTCTTCAC
    CGGGCAGCTG ATCCTAGCAC TTATCCTGCT CCTCCAGGAA CACCTAAAGT CCTGAATGTC
    AGTCGCACCA GCATTAGTCT TCGTTGGGCT AAAAGCCAAG AGAAACCCGG AGCTGTGGGC
    CCAATCATTG GATACACTGT AGAGTACTTC AGTCCGGATC TGCAAACTGG TTGGATTGTG
    GCTGCCCAAC GAGTTGGCGA CACTCAAGTC ACTATCTCGG GTCTCACTCC TGGCACTTCG
    TATGTGTTCC TAGTTAGAGC TGAGAATACT CAGGGTATTT CTGTGCCTTC CGGCTTATCA
    AATACTATTA AAACCATTGA GGCGGATTTC GATGCAGCTT CTGCCAATGA TTTGTCAGCA
    GCTCGAACTT TGCTGACGGG AAAGTCGGTG GAGCTAATAG ATGCCTCGGC TATCAATGCT
    AGTGCCGTAA GACTTGAGTG GATGCTCCAC GTGAGCGCTG ATGAGAAATA TGTAGAGGGC
    CTGCGCATAC ACTATAAGGA TGCCAGTGTA CCATCCGCAC AGTATCACTC GATCACTGTT
    ATGGATGCCT CTGCAGAATC GTTTGTGGTG GGAAACCTTA AGAAGTACAC CAAGTATGAG
    TTCTTCCTAA CACCCTTTTT TGAGACAATT GAAGGACAGC CCAGTAACTC CAAGACAGCC
    CTCACCTATG AAGATGTTCC CTCCGCACCA CCGGATAACA TTCAGATTGG CATGTACAAC
    CAAACAGCCG GTTGGGTGCG TTGGACTCCG CCACCCTCCC AGCACCACAA TGGCAATTTG
    TATGGCTACA AGATTGAGGT CAGCGCCGGT AACACCATGA AGGTGCTGGC CAATATGACT
    CTTAATGCTA CCACCACATC TGTGCTCCTA AATAACCTAA CCACCGGAGC TGTGTACAGC
    GTGAGGTTGA ACTCCTTTAC CAAGGCAGGA GATGGACCTT ACTCCAAACC GATATCACTA
    TTCATGGACC CCACCCATCA TGTGCATCCG CCACGGGCAC ATCCAAGCGG CACCCATGAT
    GGGCGACATG AGGGACAGGA TCTCACGTAT CATAACAATG GCAACATACC ACCTGGCGAC
    ATTAATCCCA CCACTCATAA AAAGACCACT GACTACCTAT CTGGACCATG GCTAATGGTG
    CTGGTCTGCA TCGTTCTTCT AGTCCTGGTT ATTTCGGCGG CTATTTCGAT GGTCTACTTC
    AAGCGCAAGC ATCAAATGAC CAAGGAATTG GGTCACTTAA GTGTGGTCAG TGACAACGAA
    ATAACCGCAT TGAACATCAA TAGCAAAGAG AGCCTTTGGA TAGACCATCA TCGTGGATGG
    CGAACTGCCG ATACTGACAA AGACTCAGGA TTAAGCGAAT CGAAGCTACT ATCCCACGTA
    AACAGCAGCC AATCCAACTA CAATAACTCC GATGGAGGAA CCGATTATGC AGAAGTTGAC
    ACCCGTAACC TTACCACCTT CTACAATTGT CGCAAGAGCC CCGATAATCC CACGCCGTAC
    GCCACCACTA TGATCATTGG TACCTCTTCC AGTGAGACCT GCACCAAGAC AACATCTATA
    AGTGCCGATA AGGACTCGGG AACTCATTCG CCCTATTCTG ACGCATTTGC CGGTCAGGTG
    CCAGCGGTTC CTGTTGTCAA ATCCAACTAT CTTCAGTATC CGGTTGAACC GATCAACTGG
    TCAGAGTTTC TACCCCCGCC GCCAGAACAC CCACCTCCGT CTTCTACCTA TGGATACGCA
    CAAGGATCTC CTGAATCTTC ACGGAAGAGC TCCAAAAGCG CAGGTTCCGG CATTTCTACA
    AATCAAAGCA TTCTGAACGC CTCCATACAC AGCAGCTCCT CGGGCGGCTT TTCAGCTTGG
    GGAGTTTCGC CCCAATATGC TGTCGCCTGT CCACCGGAAA ACGTTTATAG CAATCCGCTG
    TCGGCAGTGG CTGGCGGCAC CCAGAACCGC TATCAGATAA CGCCAACAAA CCAACATCCG
    CCACAGTTAC CGGCCTACTT TGCCACCACG GGTCCAGGAG GAGCTGTACC ACCCAACCAC
    CTGCCATTTG CCACACAGCG TCATGCAGCC AGCGAGTACC AGGCTGGACT GAATGCAGCG
    CGATGTGCCC AAAGCCGCGC CTGCAACAGC TGCGATGCCT TGGCCACACC CTCGCCCATG
    CAACCCCCAC CGCCAGTTCC CGTACCCGAG GGCTGGTACC AACCGGTGCA TCCCAATAGC
    CACCCGATGC ACCCGACCTC CTCCAACCAC CAGATCTACC AGTGCTCCTC CGAGTGCTCG
    GATCACTCGA GGAGCTCGCA GAGTCACAAG CGGCAGCTGC AGCTCGAGGA GCACGGCAGC
    AGTGCCAAAC AACGCGGAGG ACACCACCGT CGACGAGCCC CGGTGGTGCA GCCGTGCATG
    GAGAGCGAGA ACGAGAACAT GCTGGCGGAG TACGAGCAGC GCCAGTACAC CAGCGATTGT
    TGCAATAGCT CCCGCGAGGG CGACACCTGC TCCTGCAGCG AGGGATCCTG TCTTTACGCC
    GAGGCGGGCG AGCCGGCGCC TCGTCAAATG ACTGCTAAGA ACACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_726224.3
    CDS482..4669
    Translation

    Target ORF information:

    RefSeq Version NM_166543.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster roundabout 1 (robo1), transcript variant C, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_166543.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCATCCCA TGCATCCCGA AAACCACGCC ATCGCCCGGA GCACGAGCAC CACTAATAAC 
    CCATCTCGCA GTCGGAGCAG CAGGATGTGG CTCCTGCCCG CCTGGCTGCT CCTCGTCCTG
    GTGGCCAGCA ATGGCCTGCC AGCAGTCAGA GGCCAGTACC AATCGCCACG TATCATCGAG
    CATCCCACGG ATCTGGTCGT TAAGAAGAAT GAACCCGCCA CGCTCAACTG CAAAGTGGAG
    GGCAAGCCGG AACCCACCAT TGAGTGGTTT AAGGATGGCG AACCCGTCAG CACCAACGAA
    AAGAAATCGC ACCGCGTCCA GTTCAAGGAC GGCGCCCTCT TCTTCTACAG GACAATGCAG
    GGCAAGAAGG AACAGGACGG CGGAGAGTAC TGGTGCGTGG CCAAGAACCG AGTGGGCCAG
    GCCGTTAGTC GCCATGCCTC GCTCCAGATA GCTGTTTTGC GCGACGATTT TCGCGTGGAG
    CCCAAAGACA CGCGAGTGGC CAAAGGCGAG ACGGCTCTGC TGGAGTGTGG GCCGCCCAAA
    GGCATTCCAG AGCCAACGCT GATTTGGATA AAGGACGGCG TTCCCTTGGA CGACCTGAAA
    GCCATGTCGT TTGGCGCCAG CTCCCGCGTT CGAATTGTGG ACGGTGGCAA CCTGCTGATC
    AGCAATGTGG AGCCCATTGA TGAGGGCAAC TACAAGTGCA TTGCCCAGAA TCTGGTAGGC
    ACCCGCGAGA GCAGCTATGC CAAGCTGATT GTCCAGGTCA AACCATACTT TATGAAGGAG
    CCCAAGGATC AGGTGATGCT CTACGGCCAG ACAGCCACTT TCCACTGCTC AGTGGGCGGT
    GATCCGCCGC CGAAAGTGTT GTGGAAAAAG GAGGAGGGCA ATATTCCGGT GTCCAGAGCG
    CGAATCCTTC ACGACGAGAA AAGTTTAGAG ATATCCAACA TAACGCCCAC CGATGAGGGC
    ACCTATGTCT GCGAGGCACA CAACAATGTC GGTCAGATCA GCGCTAGGGC TTCTCTTATA
    GTCCATGCTC CGCCGAACTT TACGAAAAGA CCCAGTAACA AGAAAGTGGG ACTAAATGGT
    GTTGTCCAAC TACCTTGCAT GGCCTCCGGA AACCCACCGC CATCTGTTTT CTGGACCAAG
    GAAGGAGTAT CCACTCTCAT GTTCCCAAAT AGTTCGCACG GGAGGCAGCA TGTGGCTGCC
    GATGGAACTC TGCAGATTAC GGATGTGCGG CAGGAAGACG AAGGCTACTA TGTGTGTTCC
    GCTTTCAGTG TAGTCGATTC CTCTACAGTA CGGGTTTTCC TGCAAGTCAG CTCGTTAGAC
    GAGCGTCCAC CTCCGATTAT TCAAATCGGA CCTGCCAATC AAACACTGCC CAAGGGATCA
    GTTGCTACTT TACCCTGTCG GGCCACCGGA AATCCCAGTC CCCGTATTAA GTGGTTCCAC
    GATGGACATG CCGTACAAGC GGGCAATCGA TACAGCATCA TCCAAGGAAG CTCACTGAGA
    GTCGATGACC TTCAACTTAG TGACTCTGGT ACCTACACCT GCACTGCATC TGGCGAACGA
    GGAGAAACTT CCTGGGCTGC CACACTAACG GTGGAAAAAC CCGGTTCTAC ATCTCTTCAC
    CGGGCAGCTG ATCCTAGCAC TTATCCTGCT CCTCCAGGAA CACCTAAAGT CCTGAATGTC
    AGTCGCACCA GCATTAGTCT TCGTTGGGCT AAAAGCCAAG AGAAACCCGG AGCTGTGGGC
    CCAATCATTG GATACACTGT AGAGTACTTC AGTCCGGATC TGCAAACTGG TTGGATTGTG
    GCTGCCCAAC GAGTTGGCGA CACTCAAGTC ACTATCTCGG GTCTCACTCC TGGCACTTCG
    TATGTGTTCC TAGTTAGAGC TGAGAATACT CAGGGTATTT CTGTGCCTTC CGGCTTATCA
    AATACTATTA AAACCATTGA GGCGGATTTC GATGCAGCTT CTGCCAATGA TTTGTCAGCA
    GCTCGAACTT TGCTGACGGG AAAGTCGGTG GAGCTAATAG ATGCCTCGGC TATCAATGCT
    AGTGCCGTAA GACTTGAGTG GATGCTCCAC GTGAGCGCTG ATGAGAAATA TGTAGAGGGC
    CTGCGCATAC ACTATAAGGA TGCCAGTGTA CCATCCGCAC AGTATCACTC GATCACTGTT
    ATGGATGCCT CTGCAGAATC GTTTGTGGTG GGAAACCTTA AGAAGTACAC CAAGTATGAG
    TTCTTCCTAA CACCCTTTTT TGAGACAATT GAAGGACAGC CCAGTAACTC CAAGACAGCC
    CTCACCTATG AAGATGTTCC CTCCGCACCA CCGGATAACA TTCAGATTGG CATGTACAAC
    CAAACAGCCG GTTGGGTGCG TTGGACTCCG CCACCCTCCC AGCACCACAA TGGCAATTTG
    TATGGCTACA AGATTGAGGT CAGCGCCGGT AACACCATGA AGGTGCTGGC CAATATGACT
    CTTAATGCTA CCACCACATC TGTGCTCCTA AATAACCTAA CCACCGGAGC TGTGTACAGC
    GTGAGGTTGA ACTCCTTTAC CAAGGCAGGA GATGGACCTT ACTCCAAACC GATATCACTA
    TTCATGGACC CCACCCATCA TGTGCATCCG CCACGGGCAC ATCCAAGCGG CACCCATGAT
    GGGCGACATG AGGGACAGGA TCTCACGTAT CATAACAATG GCAACATACC ACCTGGCGAC
    ATTAATCCCA CCACTCATAA AAAGACCACT GACTACCTAT CTGGACCATG GCTAATGGTG
    CTGGTCTGCA TCGTTCTTCT AGTCCTGGTT ATTTCGGCGG CTATTTCGAT GGTCTACTTC
    AAGCGCAAGC ATCAAATGAC CAAGGAATTG GGTCACTTAA GTGTGGTCAG TGACAACGAA
    ATAACCGCAT TGAACATCAA TAGCAAAGAG AGCCTTTGGA TAGACCATCA TCGTGGATGG
    CGAACTGCCG ATACTGACAA AGACTCAGGA TTAAGCGAAT CGAAGCTACT ATCCCACGTA
    AACAGCAGCC AATCCAACTA CAATAACTCC GATGGAGGAA CCGATTATGC AGAAGTTGAC
    ACCCGTAACC TTACCACCTT CTACAATTGT CGCAAGAGCC CCGATAATCC CACGCCGTAC
    GCCACCACTA TGATCATTGG TACCTCTTCC AGTGAGACCT GCACCAAGAC AACATCTATA
    AGTGCCGATA AGGACTCGGG AACTCATTCG CCCTATTCTG ACGCATTTGC CGGTCAGGTG
    CCAGCGGTTC CTGTTGTCAA ATCCAACTAT CTTCAGTATC CGGTTGAACC GATCAACTGG
    TCAGAGTTTC TACCCCCGCC GCCAGAACAC CCACCTCCGT CTTCTACCTA TGGATACGCA
    CAAGGATCTC CTGAATCTTC ACGGAAGAGC TCCAAAAGCG CAGGTTCCGG CATTTCTACA
    AATCAAAGCA TTCTGAACGC CTCCATACAC AGCAGCTCCT CGGGCGGCTT TTCAGCTTGG
    GGAGTTTCGC CCCAATATGC TGTCGCCTGT CCACCGGAAA ACGTTTATAG CAATCCGCTG
    TCGGCAGTGG CTGGCGGCAC CCAGAACCGC TATCAGATAA CGCCAACAAA CCAACATCCG
    CCACAGTTAC CGGCCTACTT TGCCACCACG GGTCCAGGAG GAGCTGTACC ACCCAACCAC
    CTGCCATTTG CCACACAGCG TCATGCAGCC AGCGAGTACC AGGCTGGACT GAATGCAGCG
    CGATGTGCCC AAAGCCGCGC CTGCAACAGC TGCGATGCCT TGGCCACACC CTCGCCCATG
    CAACCCCCAC CGCCAGTTCC CGTACCCGAG GGCTGGTACC AACCGGTGCA TCCCAATAGC
    CACCCGATGC ACCCGACCTC CTCCAACCAC CAGATCTACC AGTGCTCCTC CGAGTGCTCG
    GATCACTCGA GGAGCTCGCA GAGTCACAAG CGGCAGCTGC AGCTCGAGGA GCACGGCAGC
    AGTGCCAAAC AACGCGGAGG ACACCACCGT CGACGAGCCC CGGTGGTGCA GCCGTGCATG
    GAGAGCGAGA ACGAGAACAT GCTGGCGGAG TACGAGCAGC GCCAGTACAC CAGCGATTGT
    TGCAATAGCT CCCGCGAGGG CGACACCTGC TCCTGCAGCG AGGGATCCTG TCTTTACGCC
    GAGGCGGGCG AGCCGGCGCC TCGTCAAATG ACTGCTAAGA ACACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.