RYBP cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	RYBP
Entrez Gene ID	37601
Full Name	Ring and YY1 Binding Protein
Synonyms	CG12190,Dmel\CG12190,dRYBP
General protein information	Preferred Names Ring and YY1 Binding Protein Names CG12190 gene product from transcript CG12190-RB CG12190-PA CG12190-PB RYBP-PA RYBP-PB ring and YY1 binding protein
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 58F7-58F7

mRNA and Protein(s)

mRNA	Protein	Name
NM_137861.3	NP_611705.1	ring and YY1 binding protein, isoform A
NM_001299813.1	NP_001286742.1	ring and YY1 binding protein, isoform B

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	yaf2	NP_001038733.1
Rattus norvegicus (Norway rat)	Yaf2	NP_001128343.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010288	XP_319485.2
Mus musculus (house mouse)	Yaf2	NP_077151.3
Gallus gallus (chicken)	YAF2	NP_001007851.1
Xenopus tropicalis (tropical clawed frog)	yaf2	NP_988897.1
Bos taurus (cattle)	YAF2	NP_001069547.1
Drosophila melanogaster (fruit fly)	RYBP	NP_611705.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data how that dRYBP protein is itself ubiquitylated and binds ubiquitylated proteins.
Title: dRYBP counteracts chromatin-dependent activation and repression of transcription.

dRYBP-SCF complex inhibit apoptosis in Drosophila.
Title: A novel dRYBP-SCF complex functions to inhibit apoptosis in Drosophila.

RYBP-induced apoptosis is inhibited by high levels of either p35 or Drosophila Inhibitor of Apoptosis Protein 1, and requires the function of the pro-apoptotic REAPER protein.
Title: High levels of dRYBP induce apoptosis in Drosophila imaginal cells through the activation of reaper and the requirement of trithorax, dredd and dFADD.

These results indicate that the dRYBP gene functions in the control of cell identity together with the Sex comb extra gene/trithorax gene proteins.
Title: Functional characterization of the dRYBP gene in Drosophila.

The Ring and YY1 Binding Protein (RYBP) is able to interact with Polycomb group proteins to repress transcription.
Title: The Drosophila RYBP gene functions as a Polycomb-dependent transcriptional repressor.

The following RYBP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RYBP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa12565	NM_137861.3 Latest version!	Drosophila melanogaster ring and YY1 binding protein, transcript variant A (RYBP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa12565	NM_001299813.1 Latest version!	Drosophila melanogaster ring and YY1 binding protein, transcript variant B (RYBP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RYBP ( NM_137861.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611705.1 Drosophila melanogaster ring and YY1 binding protein, transcript variant A (RYBP), mRNA.

Price & Availability↑

CloneID	OFa12565
Clone ID Related Accession (Same CDS sequence)	NM_137861.3 , NM_001299813.1
Accession Version	NM_137861.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ring and YY1 binding protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137861.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACAAGA AATCCTCGCC GGTGCGACGA CAAAAGCGGC AGGCGAAGGT GATCGAGGAG 
AACTTCTGGG ACTGCAGCGT CTGCACATAT AGAAACTCGG CGGAGGCCTT CAAGTGTCGC 
ATGTGCGACG TGCGGAAAGG AACCTCCACG CGAAAGCCAC GCCTGAACTC AGCGCTAGTC 
GCCCAGCAGG CCGCCACTTT GCCAGGAGCC AGTGTCAACA TGCCCAACGG GAAGTCCGCC 
TCCGGATCAC GGCATGGCAG TGGGCACGAC CGGCAGAGGC ACAAGCGGTA TCCGGCACGG 
TTGAAAAACG TGGACCGATC GACGGCACAG ACGCGAGAGG TGACGGTCAA CTCGGTGACC 
GTGTTCATCA CAGAGTACAA GGCCAAGCCG GTGAGCAGCC GACGCGAATC CAGTGAGCAG 
AGCTTCAGCG AGAGCAACGA CAGCCGGAGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611705.1
CDS	343..795
Translation	MDKKSSPVRRQKRQAKVIEENFWDCSVCTYRNSAEAFKCRMCDVRKGTSTRKPRLNSALVAQQAATLPGASVNMPNGKSASGSRHGSGHDRQRHKRYPARLKNVDRSTAQTREVTVNSVTVFITEYKAKPVSSRRESSEQSFSESNDSRS

Target ORF information:

RefSeq Version	NM_137861.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ring and YY1 binding protein, transcript variant A (RYBP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137861.3

ORF Insert Sequence:

ATGGACAAGA AATCCTCGCC GGTGCGACGA CAAAAGCGGC AGGCGAAGGT GATCGAGGAG 
AACTTCTGGG ACTGCAGCGT CTGCACATAT AGAAACTCGG CGGAGGCCTT CAAGTGTCGC 
ATGTGCGACG TGCGGAAAGG AACCTCCACG CGAAAGCCAC GCCTGAACTC AGCGCTAGTC 
GCCCAGCAGG CCGCCACTTT GCCAGGAGCC AGTGTCAACA TGCCCAACGG GAAGTCCGCC 
TCCGGATCAC GGCATGGCAG TGGGCACGAC CGGCAGAGGC ACAAGCGGTA TCCGGCACGG 
TTGAAAAACG TGGACCGATC GACGGCACAG ACGCGAGAGG TGACGGTCAA CTCGGTGACC 
GTGTTCATCA CAGAGTACAA GGCCAAGCCG GTGAGCAGCC GACGCGAATC CAGTGAGCAG 
AGCTTCAGCG AGAGCAACGA CAGCCGGAGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RYBP ( NM_001299813.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286742.1 Drosophila melanogaster ring and YY1 binding protein, transcript variant B (RYBP), mRNA.

Price & Availability↑

CloneID	OFa12565
Clone ID Related Accession (Same CDS sequence)	NM_137861.3 , NM_001299813.1
Accession Version	NM_001299813.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ring and YY1 binding protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACAAGA AATCCTCGCC GGTGCGACGA CAAAAGCGGC AGGCGAAGGT GATCGAGGAG 
AACTTCTGGG ACTGCAGCGT CTGCACATAT AGAAACTCGG CGGAGGCCTT CAAGTGTCGC 
ATGTGCGACG TGCGGAAAGG AACCTCCACG CGAAAGCCAC GCCTGAACTC AGCGCTAGTC 
GCCCAGCAGG CCGCCACTTT GCCAGGAGCC AGTGTCAACA TGCCCAACGG GAAGTCCGCC 
TCCGGATCAC GGCATGGCAG TGGGCACGAC CGGCAGAGGC ACAAGCGGTA TCCGGCACGG 
TTGAAAAACG TGGACCGATC GACGGCACAG ACGCGAGAGG TGACGGTCAA CTCGGTGACC 
GTGTTCATCA CAGAGTACAA GGCCAAGCCG GTGAGCAGCC GACGCGAATC CAGTGAGCAG 
AGCTTCAGCG AGAGCAACGA CAGCCGGAGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286742.1
CDS	521..973
Translation	MDKKSSPVRRQKRQAKVIEENFWDCSVCTYRNSAEAFKCRMCDVRKGTSTRKPRLNSALVAQQAATLPGASVNMPNGKSASGSRHGSGHDRQRHKRYPARLKNVDRSTAQTREVTVNSVTVFITEYKAKPVSSRRESSEQSFSESNDSRS

Target ORF information:

RefSeq Version	NM_001299813.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ring and YY1 binding protein, transcript variant B (RYBP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299813.1

ORF Insert Sequence:

ATGGACAAGA AATCCTCGCC GGTGCGACGA CAAAAGCGGC AGGCGAAGGT GATCGAGGAG 
AACTTCTGGG ACTGCAGCGT CTGCACATAT AGAAACTCGG CGGAGGCCTT CAAGTGTCGC 
ATGTGCGACG TGCGGAAAGG AACCTCCACG CGAAAGCCAC GCCTGAACTC AGCGCTAGTC 
GCCCAGCAGG CCGCCACTTT GCCAGGAGCC AGTGTCAACA TGCCCAACGG GAAGTCCGCC 
TCCGGATCAC GGCATGGCAG TGGGCACGAC CGGCAGAGGC ACAAGCGGTA TCCGGCACGG 
TTGAAAAACG TGGACCGATC GACGGCACAG ACGCGAGAGG TGACGGTCAA CTCGGTGACC 
GTGTTCATCA CAGAGTACAA GGCCAAGCCG GTGAGCAGCC GACGCGAATC CAGTGAGCAG 
AGCTTCAGCG AGAGCAACGA CAGCCGGAGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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RYBP cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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