Tbp cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tbp
Entrez Gene ID	37476
Full Name	TATA binding protein
Synonyms	CG9874,Dmel\CG9874,RBP,TBP,TBP38,TFIID,TFIIDtau,TfIID,dTBP,dTFIID,dTbp,dtbp
General protein information	Preferred Names TATA binding protein Names CG9874 gene product from transcript CG9874-RA 42kDa polypeptide CG9874-PA TATA box binding protein TATA box-binding protein TATA-binding protein TATA-box binding protein Tbp-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 57F8-57F8

mRNA and Protein(s)

mRNA	Protein	Name
NM_079081.4	NP_523805.1	TATA binding protein

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Tbp	NP_523805.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010958	XP_309748.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results indicate that a portion of Brf1 is sandwiched between Bdp1 and TBP upstream of the U6 TATA box. Furthermore, Bdp1 traverses the DNA under the N-terminal stirrup of TBP to interact with the DNA (and very likely Brf1) downstream of the TATA sequence.
Title: TFIIIB subunit locations on U6 gene promoter DNA mapped by site-specific protein-DNA photo-cross-linking.

TFIID has two disctinct modes of transcription initiation, de novo initiation and reinitiation, as revealed by the inhibitory effect of TFIID-bound tin compounds on the de novo initiation, but not on the reinitiation.
Title: Chemical perturbation of an intrinsically disordered region of TFIID distinguishes two modes of transcription initiation.

Deactivation of TBP contributes to SCA17 pathogenesis.
Title: Deactivation of TBP contributes to SCA17 pathogenesis.

in fruit flies, different classes of RNA polymerase III promoters differentially utilize TBP and TRF1 for the initiation of transcription.
Title: Differential utilization of TATA box-binding protein (TBP) and TBP-related factor 1 (TRF1) at different classes of RNA polymerase III promoters.

Aptamers generated by both selections were able to bind specifically to TBP, but the two groups showed characteristics which were clearly different in terms of their capability to compete with TATA-DNA.
Title: Perturbation of discrete sites on a single protein domain with RNA aptamers: targeting of different sides of the TATA-binding protein (TBP).

The following Tbp gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tbp cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa10842	NM_079081.4 Latest version!	Drosophila melanogaster TATA binding protein (Tbp), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tbp ( NM_079081.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523805.1 Drosophila melanogaster TATA binding protein (Tbp), mRNA.

Price & Availability↑

CloneID	OFa10842
Clone ID Related Accession (Same CDS sequence)	NM_079081.4
Accession Version	NM_079081.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	TATA binding protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_079081.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACCAAA TGCTAAGCCC CAACTTCTCG ATTCCGAGCA TCGGAACGCC GCTCCACCAG 
ATGGAAGCGG ACCAGCAGAT AGTGGCCAAT CCTGTGTACC ATCCTCCGGC TGTATCGCAG 
CCGGATTCGT TGATGCCGGC ACCCGGTTCC AGTTCCGTGC AGCACCAGCA GCAGCAACAG 
CAGTCGGACG CCAGTGGGGG ATCAGGTCTC TTTGGCCACG AACCATCGCT CCCGCTGGCG 
CACAAACAAA TGCAGAGTTA CCAGCCATCG GCCTCCTATC AGCAGCAGCA GCAGCAACAG 
CAGCTCCAGA GTCAGGCGCC CGGCGGCGGT GGGAGCACTC CGCAGTCCAT GATGCAGCCG 
CAGACGCCGC AGAGCATGAT GGCCCACATG ATGCCCATGA GTGAGCGGAG TGTGGGCGGT 
TCGGGGGCCG GAGGTGGCGG AGATGCCCTG AGCAACATCC ACCAGACGAT GGGCCCCTCC 
ACGCCGATGA CACCAGCCAC ACCAGGTTCC GCTGATCCCG GTATTGTGCC ACAACTTCAG 
AACATCGTGT CCACGGTTAA TCTGTGCTGC AAACTGGACC TCAAGAAAAT AGCATTGCAT 
GCGAGAAACG CCGAGTACAA TCCTAAGCGA TTTGCGGCTG TGATTATGCG AATCCGAGAG 
CCCCGGACCA CCGCCCTTAT TTTCAGCTCC GGCAAGATGG TGTGCACAGG GGCAAAGAGT 
GAGGACGACT CCAGACTGGC AGCGAGAAAG TATGCGCGCA TCATCCAAAA GCTCGGTTTC 
CCTGCAAAGT TCCTCGACTT TAAGATTCAA AACATGGTCG GCTCCTGCGA TGTCAAGTTC 
CCCATACGCT TGGAAGGCCT GGTGCTGACC CATTGCAACT TCAGCAGCTA CGAGCCTGAG 
CTATTTCCCG GCTTAATCTA TCGTATGGTG CGACCTCGAA TCGTGCTCCT CATCTTCGTG 
TCCGGAAAGG TGGTGCTCAC TGGAGCAAAG GTGCGGCAGG AGATCTACGA TGCCTTCGAC 
AAGATATTCC CCATTTTAAA GAAGTTCAAG AAGCAGTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523805.1
CDS	191..1252
Translation	MDQMLSPNFSIPSIGTPLHQMEADQQIVANPVYHPPAVSQPDSLMPAPGSSSVQHQQQQQQSDASGGSGLFGHEPSLPLAHKQMQSYQPSASYQQQQQQQQLQSQAPGGGGSTPQSMMQPQTPQSMMAHMMPMSERSVGGSGAGGGGDALSNIHQTMGPSTPMTPATPGSADPGIVPQLQNIVSTVNLCCKLDLKKIALHARNAEYNPKRFAAVIMRIREPRTTALIFSSGKMVCTGAKSEDDSRLAARKYARIIQKLGFPAKFLDFKIQNMVGSCDVKFPIRLEGLVLTHCNFSSYEPELFPGLIYRMVRPRIVLLIFVSGKVVLTGAKVRQEIYDAFDKIFPILKKFKKQS

Target ORF information:

RefSeq Version	NM_079081.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster TATA binding protein (Tbp), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079081.4

ORF Insert Sequence:

ATGGACCAAA TGCTAAGCCC CAACTTCTCG ATTCCGAGCA TCGGAACGCC GCTCCACCAG 
ATGGAAGCGG ACCAGCAGAT AGTGGCCAAT CCTGTGTACC ATCCTCCGGC TGTATCGCAG 
CCGGATTCGT TGATGCCGGC ACCCGGTTCC AGTTCCGTGC AGCACCAGCA GCAGCAACAG 
CAGTCGGACG CCAGTGGGGG ATCAGGTCTC TTTGGCCACG AACCATCGCT CCCGCTGGCG 
CACAAACAAA TGCAGAGTTA CCAGCCATCG GCCTCCTATC AGCAGCAGCA GCAGCAACAG 
CAGCTCCAGA GTCAGGCGCC CGGCGGCGGT GGGAGCACTC CGCAGTCCAT GATGCAGCCG 
CAGACGCCGC AGAGCATGAT GGCCCACATG ATGCCCATGA GTGAGCGGAG TGTGGGCGGT 
TCGGGGGCCG GAGGTGGCGG AGATGCCCTG AGCAACATCC ACCAGACGAT GGGCCCCTCC 
ACGCCGATGA CACCAGCCAC ACCAGGTTCC GCTGATCCCG GTATTGTGCC ACAACTTCAG 
AACATCGTGT CCACGGTTAA TCTGTGCTGC AAACTGGACC TCAAGAAAAT AGCATTGCAT 
GCGAGAAACG CCGAGTACAA TCCTAAGCGA TTTGCGGCTG TGATTATGCG AATCCGAGAG 
CCCCGGACCA CCGCCCTTAT TTTCAGCTCC GGCAAGATGG TGTGCACAGG GGCAAAGAGT 
GAGGACGACT CCAGACTGGC AGCGAGAAAG TATGCGCGCA TCATCCAAAA GCTCGGTTTC 
CCTGCAAAGT TCCTCGACTT TAAGATTCAA AACATGGTCG GCTCCTGCGA TGTCAAGTTC 
CCCATACGCT TGGAAGGCCT GGTGCTGACC CATTGCAACT TCAGCAGCTA CGAGCCTGAG 
CTATTTCCCG GCTTAATCTA TCGTATGGTG CGACCTCGAA TCGTGCTCCT CATCTTCGTG 
TCCGGAAAGG TGGTGCTCAC TGGAGCAAAG GTGCGGCAGG AGATCTACGA TGCCTTCGAC 
AAGATATTCC CCATTTTAAA GAAGTTCAAG AAGCAGTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Tbp cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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