stum cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	stum
Entrez Gene ID	37473
Full Name	stumble
Synonyms	CG15677,CG15679,CG30263,CT35864,Dmel\CG30263,mdcds_15969
General protein information	Preferred Names stumble Names CG30263 gene product from transcript CG30263-RB CG30263-PB CG30263-PD CG30263-PE stum-PB stum-PD stum-PE
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 57F8-57F8

mRNA and Protein(s)

mRNA	Protein	Name
NM_001274196.2	NP_001261125.2	stumble, isoform D
NM_001316430.1	NP_001303359.1	stumble, isoform E
NM_137759.5	NP_611603.3	stumble, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

this study has identified an evolutionarily conserved gene, stum, which is required for transduction of mechanical stimuli in a specific subpopulation of Drosophila proprioceptive neurons that sense joint angles.
Title: The stum gene is essential for mechanical sensing in proprioceptive neurons.

The following stum gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the stum cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa41317	NM_001316430.1 Latest version!	Drosophila melanogaster stumble (stum), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OFa10840	NM_001274196.2 Latest version!	Drosophila melanogaster stumble (stum), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa10840	NM_137759.5 Latest version!	Drosophila melanogaster stumble (stum), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

stum ( NM_001316430.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001303359.1 Drosophila melanogaster stumble (stum), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa41317
Clone ID Related Accession (Same CDS sequence)	NM_001316430.1
Accession Version	NM_001316430.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	stumble, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGAACG GAACGCCGAA TAACGGGGTC ATTTACACGG TGGATCTGGC CCAGGATCCC 
TTCATCGATG GCGACAAGTA CTTCTTTAGG AGCAGCAGTC AGGAGTTCCT TGAGGACAAG 
GATAATGTCA AGAAGGGCGG TCTGTGCGGA TGCTGCTCCA AGCTGTTCAT GCCCTGCCGC 
CGATCCCGCT GCTCTCGTTG CTGCCGGCGC CGTGAGCGAA ACGAATCCGC CGAGGATCAG 
CCTGTCCTGT GGAGCGGCAA CAGTAGTGCC ACCACGGCCA CCAATGTCCA AGTGATTGAC 
GAGACCAAGC CGAAGCGCAA GAAGATATCG GATTGGCTAA AGTCCAGCTG CTGCCGGAGC 
TGTCGCAAGA AACCAGCCAT CGAGGAGCAC GAAGCACATC AGGAGGAAGT GTCCAAGCGG 
ACGAACCAGG CGGCTAACAT GAGCACGGGA CCAAGGACAC GAGGCAAGTG CGGCCTCTGT 
CTGAGCAAGG TGTTCTGCTG TCGATCGGTG AACAGGGTGG ATCCCACCAC CGGCGACGAG 
ACGGAGATGA AACAGTGCTG CTTCTGCATA CCATGTCGTC GAGGCAGTCG ACCCAACAAG 
AAGGGCAGCA CCGTCTCCTG GCGGGATCAG GACCCCGAGC TGGGCATCAA GCCCACCGAG 
TCCTCGGTGG TGGAAGGCGC CTCGGAGGCA GCCATGTCAG CGGCAACCGA TGCAGGGAAT 
GACCAAGTTA AGGAGGGCTG CTGCAAGCGC TTCTGGCTGA TGTTGCTCTG CTGTCGCAAG 
AGGAAGCGCC GCGAGTCCAA CGCTAGGAGA CAGAGCATTA GGGCGCCGCC ACCCAGTGAG 
GATACGCGGA AGAAGCTACA CGTGGACCTG GTGGAGTACT CGTCCAAGAT GAAGGGAGCT 
ATTCCCGTAC TGCCGCTGTA TTTAGCCTGG TTCTGTGCCT TCTGCAATGT GGTTTTCCCA 
GGACTAGGAA CCCTGCTCTC CGGACTATTT TGCCTCTGCG TGGGCATTCC GCGTTTCTCG 
CAGTTCGACA GCGCTCGTGC AAGGATCGGA TCATTCATCA TCAACATTAT CGTGGCCGTA 
TCGCAGTTCT TCTGCGTGCT CTTCTGCTTC GTGGGATGGG GCTGGTCCAT TTGGTGGGGC 
ACCATAATGC TTAGATGTGC AAAGAAATTG AGCAAAATCA AAAAGGTGGA GCGTTTGGAG 
CTGGAGGAGG AGCAACGCCA GGCGCAACTG GCGGAGGCTG GGAAAAACGG CGTCGCCGAG 
GCGGACAAGA CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303359.1
CDS	137..1411
Translation	MLNGTPNNGVIYTVDLAQDPFIDGDKYFFRSSSQEFLEDKDNVKKGGLCGCCSKLFMPCRRSRCSRCCRRRERNESAEDQPVLWSGNSSATTATNVQVIDETKPKRKKISDWLKSSCCRSCRKKPAIEEHEAHQEEVSKRTNQAANMSTGPRTRGKCGLCLSKVFCCRSVNRVDPTTGDETEMKQCCFCIPCRRGSRPNKKGSTVSWRDQDPELGIKPTESSVVEGASEAAMSAATDAGNDQVKEGCCKRFWLMLLCCRKRKRRESNARRQSIRAPPPSEDTRKKLHVDLVEYSSKMKGAIPVLPLYLAWFCAFCNVVFPGLGTLLSGLFCLCVGIPRFSQFDSARARIGSFIINIIVAVSQFFCVLFCFVGWGWSIWWGTIMLRCAKKLSKIKKVERLELEEEQRQAQLAEAGKNGVAEADKT

Target ORF information:

RefSeq Version	NM_001316430.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster stumble (stum), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316430.1

ORF Insert Sequence:

ATGCTGAACG GAACGCCGAA TAACGGGGTC ATTTACACGG TGGATCTGGC CCAGGATCCC 
TTCATCGATG GCGACAAGTA CTTCTTTAGG AGCAGCAGTC AGGAGTTCCT TGAGGACAAG 
GATAATGTCA AGAAGGGCGG TCTGTGCGGA TGCTGCTCCA AGCTGTTCAT GCCCTGCCGC 
CGATCCCGCT GCTCTCGTTG CTGCCGGCGC CGTGAGCGAA ACGAATCCGC CGAGGATCAG 
CCTGTCCTGT GGAGCGGCAA CAGTAGTGCC ACCACGGCCA CCAATGTCCA AGTGATTGAC 
GAGACCAAGC CGAAGCGCAA GAAGATATCG GATTGGCTAA AGTCCAGCTG CTGCCGGAGC 
TGTCGCAAGA AACCAGCCAT CGAGGAGCAC GAAGCACATC AGGAGGAAGT GTCCAAGCGG 
ACGAACCAGG CGGCTAACAT GAGCACGGGA CCAAGGACAC GAGGCAAGTG CGGCCTCTGT 
CTGAGCAAGG TGTTCTGCTG TCGATCGGTG AACAGGGTGG ATCCCACCAC CGGCGACGAG 
ACGGAGATGA AACAGTGCTG CTTCTGCATA CCATGTCGTC GAGGCAGTCG ACCCAACAAG 
AAGGGCAGCA CCGTCTCCTG GCGGGATCAG GACCCCGAGC TGGGCATCAA GCCCACCGAG 
TCCTCGGTGG TGGAAGGCGC CTCGGAGGCA GCCATGTCAG CGGCAACCGA TGCAGGGAAT 
GACCAAGTTA AGGAGGGCTG CTGCAAGCGC TTCTGGCTGA TGTTGCTCTG CTGTCGCAAG 
AGGAAGCGCC GCGAGTCCAA CGCTAGGAGA CAGAGCATTA GGGCGCCGCC ACCCAGTGAG 
GATACGCGGA AGAAGCTACA CGTGGACCTG GTGGAGTACT CGTCCAAGAT GAAGGGAGCT 
ATTCCCGTAC TGCCGCTGTA TTTAGCCTGG TTCTGTGCCT TCTGCAATGT GGTTTTCCCA 
GGACTAGGAA CCCTGCTCTC CGGACTATTT TGCCTCTGCG TGGGCATTCC GCGTTTCTCG 
CAGTTCGACA GCGCTCGTGC AAGGATCGGA TCATTCATCA TCAACATTAT CGTGGCCGTA 
TCGCAGTTCT TCTGCGTGCT CTTCTGCTTC GTGGGATGGG GCTGGTCCAT TTGGTGGGGC 
ACCATAATGC TTAGATGTGC AAAGAAATTG AGCAAAATCA AAAAGGTGGA GCGTTTGGAG 
CTGGAGGAGG AGCAACGCCA GGCGCAACTG GCGGAGGCTG GGAAAAACGG CGTCGCCGAG 
GCGGACAAGA CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

stum ( NM_001274196.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261125.2 Drosophila melanogaster stumble (stum), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa10840
Clone ID Related Accession (Same CDS sequence)	NM_001274196.2 , NM_137759.5
Accession Version	NM_001274196.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5613bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	stumble, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Oct 19, 2015 this sequence version replaced NM_001274196.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGCAGA CCCTCTCGTA CTCATCATCA TCGCCATCCC CATCCCCGAT GCCCATGCCA 
TCAGCCTCAG CCGCACAGAC GCCGCCACCG AACCCGAATC TGACACCGAA TCTGAACATA 
CGTCCAAGTA CGAACCCAAT ACCACGACCC AGATCCACTG GAGGCGAGTC CGCGTTCCAG 
CCGGCCATCA TCCCGGTTGA TTATAGCTAT CGCACCCATA CCACCCGCTC CATTCTCTCC 
GCCCAATCGA TGGCACCCTC CACGTTCGCC TCCAATCAGA GCTTTGTGCG CCAGCCCTCG 
GAGTCGGATG CCCAGCCGTA TAGTAGCCTC TTTGGGGATC CGGTTCCACC GCCTCTGCAG 
CCGGATGATT ACTTCGGAAC AGCAGCATTT GAGACGTTGC GAAATGGGTC TGTGGAACTG 
CCCAGCTCCG AGGAGGATGC GCTGGTGGCC GCCATGGCGC TGAACGCACT TATTCCTGCG 
GCCAGGGAAC GCATCTTTCC GAGTGTCCCG TACTCGAGGA CTCCCCGAGG ATCCCTACTG 
CCTTATCTCC AGCCACCCGA CATCCAGATT CTGCCCAACT CGAGCGGTGG CTCCAGTTCA 
GAGCAACTGG GCTCACCTAA CTATCGGCTC CTGGCCCCCG ATGACATTCT GAACAAGCCG 
GACGTTTATG ATTTGGAGAA CGAGGTCGAG GAGTACACCG TAGTCGGTCA GAGAATGTCT 
TTCGATGATG AGGAAGATGA GCCGACGAGG ACTCCTAGCC AAGCGCCCGC CAAGTTGAGT 
GTGTCCAGCA TCACGACGGA GGACCACAGG GGATTACTCA GTGACTCCTA TTCGGGAAGG 
GATTGGTTCC GTCCGGCGCC ACAGCACTTT CCAGAGTTCA CACGCATCCC ACGATTGCGG 
CCCAGCTCCA AAATGATTAT GTCGATGAAG CAGGATGAAG GACCTCTCTC CCCACTGGCC 
ACCATGATCC AGGACATGAA CAGCTCGAGC AGCGGCGGAG AGGAGCGAGT GGCACCCAAC 
CAAAGCAGGC CCGTTAGCAG GGACGTTGTA CTCTCACCGA AGCAATATCT GGAGGAACAG 
TTGGGTCTGG AGAGAGGTAG CCAGAGGCAG TCGCTGGAGG ACTATAGAAT GCCACCTCCA 
CCGGCAGACC CGCCCCCTTT CCGTAGAACT GACTTGACGG AGCAGGATGA CAGCCGCAGT 
GTGGAGCCTG AACATAGCTT AAAGCGGGAA ATGTCCCCAG TTATCATCAG GGATTCAATA 
CCTATGGGCG GAGATTATAG AGTGGAGCAA CCTCGTCCAC ACAAAAAGAC TGCTTTCACG 
ATACTTTCCA CGGGACAGGC AGCTTCTTCT GCTCCTCGGC CTCCACAACA AGCTCCCACC 
AGAGGAAGAC ACGCCTCCAT TCAGTCGACA TCGACGACTA CCTCATCGCC GGCCGGCAGG 
CCTCAGATCA GTCCTCGACG GAGATCGATC TTTGGCTCAG AGCCGCGACT GCCTGAGCTG 
CCCTCCGCGC TGGGTTACAC CAGTCACCTC GTCCACCAGA CCACGGATGA CTCGCCACGC 
CCATCGGTTC AGTTCAATAT GAGTGGACGC CACAGTCGCA GCAAGCTGGC CATTCCGCAG 
AAGGGCATTC TCCAGCAGCG GGACTCGCTG ACCTCCTCCA TCGACACCTT CACGCCAAGA 
GCCCAAATAA GAAGGGGTTC CATTGGCAGG GATCCCATCC GCAGCTTGCC CTATGGCCAA 
AAGATCAAGA GCATGGAAAC ACTGCCCCTG ATAAACGATC AGCATCGCAG CTCCATTCCC 
CGCCTGCACT ACGGATCCAC CATGGATCTG AGTAGGCCGG AACCTGCTGT TTTTCCGCCC 
GGAGCACTGC CGCGACCACT GAAGCCAAAT CTGAATCCCA CGCGAAGGCA ACGCGGTCTC 
CTGAAGATCA TTAACAAGGA GGAGTCGCTC ACCCACAACC CGCCGCGTTC CAACTGGTGC 
AGTTTGGATG ACCTGAGCCG ACCCTCCTTC GATGGCACTC CCAACCAGGC AAGACGTCGC 
TCCAGCTCGA CCTCACCGGA GCGTCGCAGT TCCACGCAAA CGGTCTCGGA TCGAAGAAGT 
TCCAACCTGA GCAGCGTCAC CGCCACCACC TCGGATTTCA GTTTCCGCCG ACCAACGCTC 
TCCACACTGC CAGCAGCTCA GACACTGTCC AGAATGCGTC GGAAATCTGT GCAGGCTCTA 
AGTCCGAATA GGATACCCAA CAGGATACCA AGCTTTGGAC AGCGAAGGCA GTCCATCTAT 
CAGAGGGATC AGGAGCCCAA GCCGTTGAGC AGGCACGGAA AGATCGCCAA GCCGAGCACT 
CTGTCACCCA TAATTGGAAC CCCAAATAAG GACAGCAGTT CGGCACAGAG TCCAAACCAC 
AGATCCTCGC TGATGGCCGA TGCGGTGGAT ACCCCATCCG AGGTTTCCAC GCGACGGGAC 
TCGGTGTCCC GCATACCGGT TCGCAAAAGT CCAGACTCAC GCTCCAGTTC GCCACCCAAA 
GACTTTGGAA TCCCTGCCTC TGGACGAAGC TCGCGGGCTA GCATTACTAG GTCCCCTTCC 
AGAACCATGA ATGCCGGCAG TCGATCCCCT TCCAGATCCA TGCATCCCAG CAGAACGTCC 
TCGAGGGAGA GTGGAAGACC TGGAGACTCG AGGTCGGCAT CCAGGGGCCC ACCTTCTAGG 
CCTGGTTCCC GATTGGATCA GATCGGTTCC CGAATGGATC GGCCCAATTC ACGCTTGGAA 
AGATCCAGCT CACGCGCTGA GAGATCTAGT TCCCGGGCGG ATAGGCCCAG TTCCCGTTTC 
GATGTGTCCA ACTCGCGGGG AAACTCACGT GCAAACTCGC GACTGAGCAT CAACTCTTCC 
TCGCTGCGGT CGTCCAGCAT TTCACCCGCT ACCATGGCCA GGAACAGGAG CATGCGTGCC 
ACAACACCCA GTCGCCGAAA GTCCATCTCC TTGAGTCCAG CCAGTGCTAT GATGGGTCGC 
CGGAAATCAA TCAGTCCCGA TGCCGTTGGT CAAAACCGAA GAGTTTCTAG TCGGGGTAAG 
CCCGAGCCTC CAGAGATTCT ATCTCGAAGG AACTCCCGCT CGCGCATTCC CACCAGATCG 
ACTAAAGTTG CCTCCAAATC CCCGCCGTCT TCGTCCAAGA AGAGATCGAA ATCGCCGGAG 
AAACCCAAAG CGATGGGATC TACGCCCAAG AGCAACAAAG TTGGAAAAGG GGTAACCGCT 
AAAGGAAGCC CCAAAGCGAA GCCGAAGATC GCTGCAAGTG CCAAACCAAA GACCAAGACG 
GAATCCTTGA TGAAACCACC AGTGAAGAAT GCAAACAAAA CTCCTGTAAA TGGTAGACCA 
ACCACAAATG CAAAAAAGCC AGCCAGCAAG GCGATTTCCA CAAAGGATGC AAAAGTGGAG 
AAGGGAAAGG AGACTGGGAA GTCTGTGGCC TCCAAGAGCA ATGGTAAAGA AACCGAGTCT 
TCAGTAAAAT CCAGTAAATC CCAAGTCACA AAAACTAAAC CACCTGGGGG AACAACATCG 
GAGGCCAAGA AACCAGCCAC CACAAAAACC CATGGCATAA CTTTAGAGAA AGGGACAAAT 
GGAACCGATC CCGTTGGAGG AATTCCTCCT TTGGCGGCTC AGGTTGGCAA TGCAGTTCTG 
CAGGCGGTGG AAGCGGTTCC TGCGGTTACC AGTGAGGTTA CCGTGCCCTC AACGCCAGGA 
GGTAAAGGTG CTGGCGGCGC CAATATGTCG AAACTGGTGC GAATGAGCTC CAGGATGAGC 
CTGCTGAGCA ACAAGAGCAC CAAGGTGCGA TCAGATTCTC CTCAAAACCG AAAGGTGGCC 
ACCGTACAGG AACATGCTAC AGAAAAAGAA GAAACAGTGG GAGCCAGCTC GCAACCCATG 
TCCAATGATG CTGCTGCTAT TCTGGAAAAG TCGCAGAAAA CTCTGGAGAA CATTCAAAAG 
ACCGTAACTG AGGCCACCGA CGAGATCCAT AAGACCATCA GCGAAAATCT CACCGATCTG 
AAAAGTCTGG AGAACGACCT TGCCGCTGAT CCCTCGCCAA CGCCCAGTTC GGGTACCACG 
ATGGCCAGGC CGGGTGAATC CGCCTCCCGG ACGGGCACAG CCGACGGCAG CATTGCTCCT 
CAGAGTTCGG GGGAGCTGCC CAAGTCACCG TTTCCACCCA CCCAACCCAT CGAAGCCTCT 
GTCTCGGTTT TGCATCCCAA TGAAAGCTCC GCCGAGCGAA TAGCCAGCGT GCCCGAGGTG 
GAGTGCGAAA GCTCTGATCT TCCAACGGCC CTAGACGCTT CCGAATCCGA GCTGGTTGGT 
AATGTTATGC CCACGCCCGA GAGTGGAGCG GACTTCAAAG TGGACGCCAA GCGGCGATCG 
CCGGACGGAC AGGGAGGATC CACGGCGGGA AGCGGCGGTC TGGCAAAGAG CAGCAGTCAG 
GAGTTCCTTG AGGACAAGGA TAATGTCAAG AAGGGCGGTC TGTGCGGATG CTGCTCCAAG 
CTGTTCATGC CCTGCCGCCG ATCCCGCTGC TCTCGTTGCT GCCGGCGCCG TGAGCGAAAC 
GAATCCGCCG AGGATCAGCC TGTCCTGTGG AGCGGCAACA GTAGTGCCAC CACGGCCACC 
AATGTCCAAG TGATTGACGA GACCAAGCCG AAGCGCAAGA AGATATCGGA TTGGCTAAAG 
TCCAGCTGCT GCCGGAGCTG TCGCAAGAAA CCAGCCATCG AGGAGCACGA AGCACATCAG 
GAGGAAGTGT CCAAGCGGAC GAACCAGGCG GCTAACATGA GCACGGGACC AAGGACACGA 
GGCAAGTGCG GCCTCTGTCT GAGCAAGGTG TTCTGCTGTC GATCGGTGAA CAGGGTGGAT 
CCCACCACCG GCGACGAGAC GGAGATGAAA CAGTGCTGCT TCTGCATACC ATGTCGTCGA 
GGCAGTCGAC CCAACAAGAA GGGCAGCACC GTCTCCTGGC GGGATCAGGA CCCCGAGCTG 
GGCATCAAGC CCACCGAGTC CTCGGTGGTG GAAGGCGCCT CGGAGGCAGC CATGTCAGCG 
GCAACCGATG CAGGGAATGA CCAAGTTAAG GAGGGCTGCT GCAAGCGCTT CTGGCTGATG 
TTGCTCTGCT GTCGCAAGAG GAAGCGCCGC GAGTCCAACG CTAGGAGACA GAGCATTAGG 
GCGCCGCCAC CCAGTGAGGA TACGCGGAAG AAGCTACACG TGGACCTGGT GGAGTACTCG 
TCCAAGATGA AGGGAGCTAT TCCCGTACTG CCGCTGTATT TAGCCTGGTT CTGTGCCTTC 
TGCAATGTGG TTTTCCCAGG ACTAGGAACC CTGCTCTCCG GACTATTTTG CCTCTGCGTG 
GGCATTCCGC GTTTCTCGCA GTTCGACAGC GCTCGTGCAA GGATCGGATC ATTCATCATC 
AACATTATCG TGGCCGTATC GCAGTTCTTC TGCGTGCTCT TCTGCTTCGT GGGATGGGGC 
TGGTCCATTT GGTGGGGCAC CATAATGCTT AGATGTGCAA AGAAATTGAG CAAAATCAAA 
AAGGTGGAGC GTTTGGAGCT GGAGGAGGAG CAACGCCAGG CGCAACTGGC GGAGGCTGGG 
AAAAACGGCG TCGCCGAGGC GGACAAGACA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261125.2
CDS	442..6054
Translation	MQQTLSYSSSSPSPSPMPMPSASAAQTPPPNPNLTPNLNIRPSTNPIPRPRSTGGESAFQPAIIPVDYSYRTHTTRSILSAQSMAPSTFASNQSFVRQPSESDAQPYSSLFGDPVPPPLQPDDYFGTAAFETLRNGSVELPSSEEDALVAAMALNALIPAARERIFPSVPYSRTPRGSLLPYLQPPDIQILPNSSGGSSSEQLGSPNYRLLAPDDILNKPDVYDLENEVEEYTVVGQRMSFDDEEDEPTRTPSQAPAKLSVSSITTEDHRGLLSDSYSGRDWFRPAPQHFPEFTRIPRLRPSSKMIMSMKQDEGPLSPLATMIQDMNSSSSGGEERVAPNQSRPVSRDVVLSPKQYLEEQLGLERGSQRQSLEDYRMPPPPADPPPFRRTDLTEQDDSRSVEPEHSLKREMSPVIIRDSIPMGGDYRVEQPRPHKKTAFTILSTGQAASSAPRPPQQAPTRGRHASIQSTSTTTSSPAGRPQISPRRRSIFGSEPRLPELPSALGYTSHLVHQTTDDSPRPSVQFNMSGRHSRSKLAIPQKGILQQRDSLTSSIDTFTPRAQIRRGSIGRDPIRSLPYGQKIKSMETLPLINDQHRSSIPRLHYGSTMDLSRPEPAVFPPGALPRPLKPNLNPTRRQRGLLKIINKEESLTHNPPRSNWCSLDDLSRPSFDGTPNQARRRSSSTSPERRSSTQTVSDRRSSNLSSVTATTSDFSFRRPTLSTLPAAQTLSRMRRKSVQALSPNRIPNRIPSFGQRRQSIYQRDQEPKPLSRHGKIAKPSTLSPIIGTPNKDSSSAQSPNHRSSLMADAVDTPSEVSTRRDSVSRIPVRKSPDSRSSSPPKDFGIPASGRSSRASITRSPSRTMNAGSRSPSRSMHPSRTSSRESGRPGDSRSASRGPPSRPGSRLDQIGSRMDRPNSRLERSSSRAERSSSRADRPSSRFDVSNSRGNSRANSRLSINSSSLRSSSISPATMARNRSMRATTPSRRKSISLSPASAMMGRRKSISPDAVGQNRRVSSRGKPEPPEILSRRNSRSRIPTRSTKVASKSPPSSSKKRSKSPEKPKAMGSTPKSNKVGKGVTAKGSPKAKPKIAASAKPKTKTESLMKPPVKNANKTPVNGRPTTNAKKPASKAISTKDAKVEKGKETGKSVASKSNGKETESSVKSSKSQVTKTKPPGGTTSEAKKPATTKTHGITLEKGTNGTDPVGGIPPLAAQVGNAVLQAVEAVPAVTSEVTVPSTPGGKGAGGANMSKLVRMSSRMSLLSNKSTKVRSDSPQNRKVATVQEHATEKEETVGASSQPMSNDAAAILEKSQKTLENIQKTVTEATDEIHKTISENLTDLKSLENDLAADPSPTPSSGTTMARPGESASRTGTADGSIAPQSSGELPKSPFPPTQPIEASVSVLHPNESSAERIASVPEVECESSDLPTALDASESELVGNVMPTPESGADFKVDAKRRSPDGQGGSTAGSGGLAKSSSQEFLEDKDNVKKGGLCGCCSKLFMPCRRSRCSRCCRRRERNESAEDQPVLWSGNSSATTATNVQVIDETKPKRKKISDWLKSSCCRSCRKKPAIEEHEAHQEEVSKRTNQAANMSTGPRTRGKCGLCLSKVFCCRSVNRVDPTTGDETEMKQCCFCIPCRRGSRPNKKGSTVSWRDQDPELGIKPTESSVVEGASEAAMSAATDAGNDQVKEGCCKRFWLMLLCCRKRKRRESNARRQSIRAPPPSEDTRKKLHVDLVEYSSKMKGAIPVLPLYLAWFCAFCNVVFPGLGTLLSGLFCLCVGIPRFSQFDSARARIGSFIINIIVAVSQFFCVLFCFVGWGWSIWWGTIMLRCAKKLSKIKKVERLELEEEQRQAQLAEAGKNGVAEADKT

Target ORF information:

RefSeq Version	NM_001274196.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster stumble (stum), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274196.2

ORF Insert Sequence:

ATGCAGCAGA CCCTCTCGTA CTCATCATCA TCGCCATCCC CATCCCCGAT GCCCATGCCA 
TCAGCCTCAG CCGCACAGAC GCCGCCACCG AACCCGAATC TGACACCGAA TCTGAACATA 
CGTCCAAGTA CGAACCCAAT ACCACGACCC AGATCCACTG GAGGCGAGTC CGCGTTCCAG 
CCGGCCATCA TCCCGGTTGA TTATAGCTAT CGCACCCATA CCACCCGCTC CATTCTCTCC 
GCCCAATCGA TGGCACCCTC CACGTTCGCC TCCAATCAGA GCTTTGTGCG CCAGCCCTCG 
GAGTCGGATG CCCAGCCGTA TAGTAGCCTC TTTGGGGATC CGGTTCCACC GCCTCTGCAG 
CCGGATGATT ACTTCGGAAC AGCAGCATTT GAGACGTTGC GAAATGGGTC TGTGGAACTG 
CCCAGCTCCG AGGAGGATGC GCTGGTGGCC GCCATGGCGC TGAACGCACT TATTCCTGCG 
GCCAGGGAAC GCATCTTTCC GAGTGTCCCG TACTCGAGGA CTCCCCGAGG ATCCCTACTG 
CCTTATCTCC AGCCACCCGA CATCCAGATT CTGCCCAACT CGAGCGGTGG CTCCAGTTCA 
GAGCAACTGG GCTCACCTAA CTATCGGCTC CTGGCCCCCG ATGACATTCT GAACAAGCCG 
GACGTTTATG ATTTGGAGAA CGAGGTCGAG GAGTACACCG TAGTCGGTCA GAGAATGTCT 
TTCGATGATG AGGAAGATGA GCCGACGAGG ACTCCTAGCC AAGCGCCCGC CAAGTTGAGT 
GTGTCCAGCA TCACGACGGA GGACCACAGG GGATTACTCA GTGACTCCTA TTCGGGAAGG 
GATTGGTTCC GTCCGGCGCC ACAGCACTTT CCAGAGTTCA CACGCATCCC ACGATTGCGG 
CCCAGCTCCA AAATGATTAT GTCGATGAAG CAGGATGAAG GACCTCTCTC CCCACTGGCC 
ACCATGATCC AGGACATGAA CAGCTCGAGC AGCGGCGGAG AGGAGCGAGT GGCACCCAAC 
CAAAGCAGGC CCGTTAGCAG GGACGTTGTA CTCTCACCGA AGCAATATCT GGAGGAACAG 
TTGGGTCTGG AGAGAGGTAG CCAGAGGCAG TCGCTGGAGG ACTATAGAAT GCCACCTCCA 
CCGGCAGACC CGCCCCCTTT CCGTAGAACT GACTTGACGG AGCAGGATGA CAGCCGCAGT 
GTGGAGCCTG AACATAGCTT AAAGCGGGAA ATGTCCCCAG TTATCATCAG GGATTCAATA 
CCTATGGGCG GAGATTATAG AGTGGAGCAA CCTCGTCCAC ACAAAAAGAC TGCTTTCACG 
ATACTTTCCA CGGGACAGGC AGCTTCTTCT GCTCCTCGGC CTCCACAACA AGCTCCCACC 
AGAGGAAGAC ACGCCTCCAT TCAGTCGACA TCGACGACTA CCTCATCGCC GGCCGGCAGG 
CCTCAGATCA GTCCTCGACG GAGATCGATC TTTGGCTCAG AGCCGCGACT GCCTGAGCTG 
CCCTCCGCGC TGGGTTACAC CAGTCACCTC GTCCACCAGA CCACGGATGA CTCGCCACGC 
CCATCGGTTC AGTTCAATAT GAGTGGACGC CACAGTCGCA GCAAGCTGGC CATTCCGCAG 
AAGGGCATTC TCCAGCAGCG GGACTCGCTG ACCTCCTCCA TCGACACCTT CACGCCAAGA 
GCCCAAATAA GAAGGGGTTC CATTGGCAGG GATCCCATCC GCAGCTTGCC CTATGGCCAA 
AAGATCAAGA GCATGGAAAC ACTGCCCCTG ATAAACGATC AGCATCGCAG CTCCATTCCC 
CGCCTGCACT ACGGATCCAC CATGGATCTG AGTAGGCCGG AACCTGCTGT TTTTCCGCCC 
GGAGCACTGC CGCGACCACT GAAGCCAAAT CTGAATCCCA CGCGAAGGCA ACGCGGTCTC 
CTGAAGATCA TTAACAAGGA GGAGTCGCTC ACCCACAACC CGCCGCGTTC CAACTGGTGC 
AGTTTGGATG ACCTGAGCCG ACCCTCCTTC GATGGCACTC CCAACCAGGC AAGACGTCGC 
TCCAGCTCGA CCTCACCGGA GCGTCGCAGT TCCACGCAAA CGGTCTCGGA TCGAAGAAGT 
TCCAACCTGA GCAGCGTCAC CGCCACCACC TCGGATTTCA GTTTCCGCCG ACCAACGCTC 
TCCACACTGC CAGCAGCTCA GACACTGTCC AGAATGCGTC GGAAATCTGT GCAGGCTCTA 
AGTCCGAATA GGATACCCAA CAGGATACCA AGCTTTGGAC AGCGAAGGCA GTCCATCTAT 
CAGAGGGATC AGGAGCCCAA GCCGTTGAGC AGGCACGGAA AGATCGCCAA GCCGAGCACT 
CTGTCACCCA TAATTGGAAC CCCAAATAAG GACAGCAGTT CGGCACAGAG TCCAAACCAC 
AGATCCTCGC TGATGGCCGA TGCGGTGGAT ACCCCATCCG AGGTTTCCAC GCGACGGGAC 
TCGGTGTCCC GCATACCGGT TCGCAAAAGT CCAGACTCAC GCTCCAGTTC GCCACCCAAA 
GACTTTGGAA TCCCTGCCTC TGGACGAAGC TCGCGGGCTA GCATTACTAG GTCCCCTTCC 
AGAACCATGA ATGCCGGCAG TCGATCCCCT TCCAGATCCA TGCATCCCAG CAGAACGTCC 
TCGAGGGAGA GTGGAAGACC TGGAGACTCG AGGTCGGCAT CCAGGGGCCC ACCTTCTAGG 
CCTGGTTCCC GATTGGATCA GATCGGTTCC CGAATGGATC GGCCCAATTC ACGCTTGGAA 
AGATCCAGCT CACGCGCTGA GAGATCTAGT TCCCGGGCGG ATAGGCCCAG TTCCCGTTTC 
GATGTGTCCA ACTCGCGGGG AAACTCACGT GCAAACTCGC GACTGAGCAT CAACTCTTCC 
TCGCTGCGGT CGTCCAGCAT TTCACCCGCT ACCATGGCCA GGAACAGGAG CATGCGTGCC 
ACAACACCCA GTCGCCGAAA GTCCATCTCC TTGAGTCCAG CCAGTGCTAT GATGGGTCGC 
CGGAAATCAA TCAGTCCCGA TGCCGTTGGT CAAAACCGAA GAGTTTCTAG TCGGGGTAAG 
CCCGAGCCTC CAGAGATTCT ATCTCGAAGG AACTCCCGCT CGCGCATTCC CACCAGATCG 
ACTAAAGTTG CCTCCAAATC CCCGCCGTCT TCGTCCAAGA AGAGATCGAA ATCGCCGGAG 
AAACCCAAAG CGATGGGATC TACGCCCAAG AGCAACAAAG TTGGAAAAGG GGTAACCGCT 
AAAGGAAGCC CCAAAGCGAA GCCGAAGATC GCTGCAAGTG CCAAACCAAA GACCAAGACG 
GAATCCTTGA TGAAACCACC AGTGAAGAAT GCAAACAAAA CTCCTGTAAA TGGTAGACCA 
ACCACAAATG CAAAAAAGCC AGCCAGCAAG GCGATTTCCA CAAAGGATGC AAAAGTGGAG 
AAGGGAAAGG AGACTGGGAA GTCTGTGGCC TCCAAGAGCA ATGGTAAAGA AACCGAGTCT 
TCAGTAAAAT CCAGTAAATC CCAAGTCACA AAAACTAAAC CACCTGGGGG AACAACATCG 
GAGGCCAAGA AACCAGCCAC CACAAAAACC CATGGCATAA CTTTAGAGAA AGGGACAAAT 
GGAACCGATC CCGTTGGAGG AATTCCTCCT TTGGCGGCTC AGGTTGGCAA TGCAGTTCTG 
CAGGCGGTGG AAGCGGTTCC TGCGGTTACC AGTGAGGTTA CCGTGCCCTC AACGCCAGGA 
GGTAAAGGTG CTGGCGGCGC CAATATGTCG AAACTGGTGC GAATGAGCTC CAGGATGAGC 
CTGCTGAGCA ACAAGAGCAC CAAGGTGCGA TCAGATTCTC CTCAAAACCG AAAGGTGGCC 
ACCGTACAGG AACATGCTAC AGAAAAAGAA GAAACAGTGG GAGCCAGCTC GCAACCCATG 
TCCAATGATG CTGCTGCTAT TCTGGAAAAG TCGCAGAAAA CTCTGGAGAA CATTCAAAAG 
ACCGTAACTG AGGCCACCGA CGAGATCCAT AAGACCATCA GCGAAAATCT CACCGATCTG 
AAAAGTCTGG AGAACGACCT TGCCGCTGAT CCCTCGCCAA CGCCCAGTTC GGGTACCACG 
ATGGCCAGGC CGGGTGAATC CGCCTCCCGG ACGGGCACAG CCGACGGCAG CATTGCTCCT 
CAGAGTTCGG GGGAGCTGCC CAAGTCACCG TTTCCACCCA CCCAACCCAT CGAAGCCTCT 
GTCTCGGTTT TGCATCCCAA TGAAAGCTCC GCCGAGCGAA TAGCCAGCGT GCCCGAGGTG 
GAGTGCGAAA GCTCTGATCT TCCAACGGCC CTAGACGCTT CCGAATCCGA GCTGGTTGGT 
AATGTTATGC CCACGCCCGA GAGTGGAGCG GACTTCAAAG TGGACGCCAA GCGGCGATCG 
CCGGACGGAC AGGGAGGATC CACGGCGGGA AGCGGCGGTC TGGCAAAGAG CAGCAGTCAG 
GAGTTCCTTG AGGACAAGGA TAATGTCAAG AAGGGCGGTC TGTGCGGATG CTGCTCCAAG 
CTGTTCATGC CCTGCCGCCG ATCCCGCTGC TCTCGTTGCT GCCGGCGCCG TGAGCGAAAC 
GAATCCGCCG AGGATCAGCC TGTCCTGTGG AGCGGCAACA GTAGTGCCAC CACGGCCACC 
AATGTCCAAG TGATTGACGA GACCAAGCCG AAGCGCAAGA AGATATCGGA TTGGCTAAAG 
TCCAGCTGCT GCCGGAGCTG TCGCAAGAAA CCAGCCATCG AGGAGCACGA AGCACATCAG 
GAGGAAGTGT CCAAGCGGAC GAACCAGGCG GCTAACATGA GCACGGGACC AAGGACACGA 
GGCAAGTGCG GCCTCTGTCT GAGCAAGGTG TTCTGCTGTC GATCGGTGAA CAGGGTGGAT 
CCCACCACCG GCGACGAGAC GGAGATGAAA CAGTGCTGCT TCTGCATACC ATGTCGTCGA 
GGCAGTCGAC CCAACAAGAA GGGCAGCACC GTCTCCTGGC GGGATCAGGA CCCCGAGCTG 
GGCATCAAGC CCACCGAGTC CTCGGTGGTG GAAGGCGCCT CGGAGGCAGC CATGTCAGCG 
GCAACCGATG CAGGGAATGA CCAAGTTAAG GAGGGCTGCT GCAAGCGCTT CTGGCTGATG 
TTGCTCTGCT GTCGCAAGAG GAAGCGCCGC GAGTCCAACG CTAGGAGACA GAGCATTAGG 
GCGCCGCCAC CCAGTGAGGA TACGCGGAAG AAGCTACACG TGGACCTGGT GGAGTACTCG 
TCCAAGATGA AGGGAGCTAT TCCCGTACTG CCGCTGTATT TAGCCTGGTT CTGTGCCTTC 
TGCAATGTGG TTTTCCCAGG ACTAGGAACC CTGCTCTCCG GACTATTTTG CCTCTGCGTG 
GGCATTCCGC GTTTCTCGCA GTTCGACAGC GCTCGTGCAA GGATCGGATC ATTCATCATC 
AACATTATCG TGGCCGTATC GCAGTTCTTC TGCGTGCTCT TCTGCTTCGT GGGATGGGGC 
TGGTCCATTT GGTGGGGCAC CATAATGCTT AGATGTGCAA AGAAATTGAG CAAAATCAAA 
AAGGTGGAGC GTTTGGAGCT GGAGGAGGAG CAACGCCAGG CGCAACTGGC GGAGGCTGGG 
AAAAACGGCG TCGCCGAGGC GGACAAGACA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

stum ( NM_137759.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611603.3 Drosophila melanogaster stumble (stum), transcript variant B, mRNA.

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CloneID	OFa10840
Clone ID Related Accession (Same CDS sequence)	NM_001274196.2 , NM_137759.5
Accession Version	NM_137759.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5613bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	stumble, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_137759.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGCAGA CCCTCTCGTA CTCATCATCA TCGCCATCCC CATCCCCGAT GCCCATGCCA 
TCAGCCTCAG CCGCACAGAC GCCGCCACCG AACCCGAATC TGACACCGAA TCTGAACATA 
CGTCCAAGTA CGAACCCAAT ACCACGACCC AGATCCACTG GAGGCGAGTC CGCGTTCCAG 
CCGGCCATCA TCCCGGTTGA TTATAGCTAT CGCACCCATA CCACCCGCTC CATTCTCTCC 
GCCCAATCGA TGGCACCCTC CACGTTCGCC TCCAATCAGA GCTTTGTGCG CCAGCCCTCG 
GAGTCGGATG CCCAGCCGTA TAGTAGCCTC TTTGGGGATC CGGTTCCACC GCCTCTGCAG 
CCGGATGATT ACTTCGGAAC AGCAGCATTT GAGACGTTGC GAAATGGGTC TGTGGAACTG 
CCCAGCTCCG AGGAGGATGC GCTGGTGGCC GCCATGGCGC TGAACGCACT TATTCCTGCG 
GCCAGGGAAC GCATCTTTCC GAGTGTCCCG TACTCGAGGA CTCCCCGAGG ATCCCTACTG 
CCTTATCTCC AGCCACCCGA CATCCAGATT CTGCCCAACT CGAGCGGTGG CTCCAGTTCA 
GAGCAACTGG GCTCACCTAA CTATCGGCTC CTGGCCCCCG ATGACATTCT GAACAAGCCG 
GACGTTTATG ATTTGGAGAA CGAGGTCGAG GAGTACACCG TAGTCGGTCA GAGAATGTCT 
TTCGATGATG AGGAAGATGA GCCGACGAGG ACTCCTAGCC AAGCGCCCGC CAAGTTGAGT 
GTGTCCAGCA TCACGACGGA GGACCACAGG GGATTACTCA GTGACTCCTA TTCGGGAAGG 
GATTGGTTCC GTCCGGCGCC ACAGCACTTT CCAGAGTTCA CACGCATCCC ACGATTGCGG 
CCCAGCTCCA AAATGATTAT GTCGATGAAG CAGGATGAAG GACCTCTCTC CCCACTGGCC 
ACCATGATCC AGGACATGAA CAGCTCGAGC AGCGGCGGAG AGGAGCGAGT GGCACCCAAC 
CAAAGCAGGC CCGTTAGCAG GGACGTTGTA CTCTCACCGA AGCAATATCT GGAGGAACAG 
TTGGGTCTGG AGAGAGGTAG CCAGAGGCAG TCGCTGGAGG ACTATAGAAT GCCACCTCCA 
CCGGCAGACC CGCCCCCTTT CCGTAGAACT GACTTGACGG AGCAGGATGA CAGCCGCAGT 
GTGGAGCCTG AACATAGCTT AAAGCGGGAA ATGTCCCCAG TTATCATCAG GGATTCAATA 
CCTATGGGCG GAGATTATAG AGTGGAGCAA CCTCGTCCAC ACAAAAAGAC TGCTTTCACG 
ATACTTTCCA CGGGACAGGC AGCTTCTTCT GCTCCTCGGC CTCCACAACA AGCTCCCACC 
AGAGGAAGAC ACGCCTCCAT TCAGTCGACA TCGACGACTA CCTCATCGCC GGCCGGCAGG 
CCTCAGATCA GTCCTCGACG GAGATCGATC TTTGGCTCAG AGCCGCGACT GCCTGAGCTG 
CCCTCCGCGC TGGGTTACAC CAGTCACCTC GTCCACCAGA CCACGGATGA CTCGCCACGC 
CCATCGGTTC AGTTCAATAT GAGTGGACGC CACAGTCGCA GCAAGCTGGC CATTCCGCAG 
AAGGGCATTC TCCAGCAGCG GGACTCGCTG ACCTCCTCCA TCGACACCTT CACGCCAAGA 
GCCCAAATAA GAAGGGGTTC CATTGGCAGG GATCCCATCC GCAGCTTGCC CTATGGCCAA 
AAGATCAAGA GCATGGAAAC ACTGCCCCTG ATAAACGATC AGCATCGCAG CTCCATTCCC 
CGCCTGCACT ACGGATCCAC CATGGATCTG AGTAGGCCGG AACCTGCTGT TTTTCCGCCC 
GGAGCACTGC CGCGACCACT GAAGCCAAAT CTGAATCCCA CGCGAAGGCA ACGCGGTCTC 
CTGAAGATCA TTAACAAGGA GGAGTCGCTC ACCCACAACC CGCCGCGTTC CAACTGGTGC 
AGTTTGGATG ACCTGAGCCG ACCCTCCTTC GATGGCACTC CCAACCAGGC AAGACGTCGC 
TCCAGCTCGA CCTCACCGGA GCGTCGCAGT TCCACGCAAA CGGTCTCGGA TCGAAGAAGT 
TCCAACCTGA GCAGCGTCAC CGCCACCACC TCGGATTTCA GTTTCCGCCG ACCAACGCTC 
TCCACACTGC CAGCAGCTCA GACACTGTCC AGAATGCGTC GGAAATCTGT GCAGGCTCTA 
AGTCCGAATA GGATACCCAA CAGGATACCA AGCTTTGGAC AGCGAAGGCA GTCCATCTAT 
CAGAGGGATC AGGAGCCCAA GCCGTTGAGC AGGCACGGAA AGATCGCCAA GCCGAGCACT 
CTGTCACCCA TAATTGGAAC CCCAAATAAG GACAGCAGTT CGGCACAGAG TCCAAACCAC 
AGATCCTCGC TGATGGCCGA TGCGGTGGAT ACCCCATCCG AGGTTTCCAC GCGACGGGAC 
TCGGTGTCCC GCATACCGGT TCGCAAAAGT CCAGACTCAC GCTCCAGTTC GCCACCCAAA 
GACTTTGGAA TCCCTGCCTC TGGACGAAGC TCGCGGGCTA GCATTACTAG GTCCCCTTCC 
AGAACCATGA ATGCCGGCAG TCGATCCCCT TCCAGATCCA TGCATCCCAG CAGAACGTCC 
TCGAGGGAGA GTGGAAGACC TGGAGACTCG AGGTCGGCAT CCAGGGGCCC ACCTTCTAGG 
CCTGGTTCCC GATTGGATCA GATCGGTTCC CGAATGGATC GGCCCAATTC ACGCTTGGAA 
AGATCCAGCT CACGCGCTGA GAGATCTAGT TCCCGGGCGG ATAGGCCCAG TTCCCGTTTC 
GATGTGTCCA ACTCGCGGGG AAACTCACGT GCAAACTCGC GACTGAGCAT CAACTCTTCC 
TCGCTGCGGT CGTCCAGCAT TTCACCCGCT ACCATGGCCA GGAACAGGAG CATGCGTGCC 
ACAACACCCA GTCGCCGAAA GTCCATCTCC TTGAGTCCAG CCAGTGCTAT GATGGGTCGC 
CGGAAATCAA TCAGTCCCGA TGCCGTTGGT CAAAACCGAA GAGTTTCTAG TCGGGGTAAG 
CCCGAGCCTC CAGAGATTCT ATCTCGAAGG AACTCCCGCT CGCGCATTCC CACCAGATCG 
ACTAAAGTTG CCTCCAAATC CCCGCCGTCT TCGTCCAAGA AGAGATCGAA ATCGCCGGAG 
AAACCCAAAG CGATGGGATC TACGCCCAAG AGCAACAAAG TTGGAAAAGG GGTAACCGCT 
AAAGGAAGCC CCAAAGCGAA GCCGAAGATC GCTGCAAGTG CCAAACCAAA GACCAAGACG 
GAATCCTTGA TGAAACCACC AGTGAAGAAT GCAAACAAAA CTCCTGTAAA TGGTAGACCA 
ACCACAAATG CAAAAAAGCC AGCCAGCAAG GCGATTTCCA CAAAGGATGC AAAAGTGGAG 
AAGGGAAAGG AGACTGGGAA GTCTGTGGCC TCCAAGAGCA ATGGTAAAGA AACCGAGTCT 
TCAGTAAAAT CCAGTAAATC CCAAGTCACA AAAACTAAAC CACCTGGGGG AACAACATCG 
GAGGCCAAGA AACCAGCCAC CACAAAAACC CATGGCATAA CTTTAGAGAA AGGGACAAAT 
GGAACCGATC CCGTTGGAGG AATTCCTCCT TTGGCGGCTC AGGTTGGCAA TGCAGTTCTG 
CAGGCGGTGG AAGCGGTTCC TGCGGTTACC AGTGAGGTTA CCGTGCCCTC AACGCCAGGA 
GGTAAAGGTG CTGGCGGCGC CAATATGTCG AAACTGGTGC GAATGAGCTC CAGGATGAGC 
CTGCTGAGCA ACAAGAGCAC CAAGGTGCGA TCAGATTCTC CTCAAAACCG AAAGGTGGCC 
ACCGTACAGG AACATGCTAC AGAAAAAGAA GAAACAGTGG GAGCCAGCTC GCAACCCATG 
TCCAATGATG CTGCTGCTAT TCTGGAAAAG TCGCAGAAAA CTCTGGAGAA CATTCAAAAG 
ACCGTAACTG AGGCCACCGA CGAGATCCAT AAGACCATCA GCGAAAATCT CACCGATCTG 
AAAAGTCTGG AGAACGACCT TGCCGCTGAT CCCTCGCCAA CGCCCAGTTC GGGTACCACG 
ATGGCCAGGC CGGGTGAATC CGCCTCCCGG ACGGGCACAG CCGACGGCAG CATTGCTCCT 
CAGAGTTCGG GGGAGCTGCC CAAGTCACCG TTTCCACCCA CCCAACCCAT CGAAGCCTCT 
GTCTCGGTTT TGCATCCCAA TGAAAGCTCC GCCGAGCGAA TAGCCAGCGT GCCCGAGGTG 
GAGTGCGAAA GCTCTGATCT TCCAACGGCC CTAGACGCTT CCGAATCCGA GCTGGTTGGT 
AATGTTATGC CCACGCCCGA GAGTGGAGCG GACTTCAAAG TGGACGCCAA GCGGCGATCG 
CCGGACGGAC AGGGAGGATC CACGGCGGGA AGCGGCGGTC TGGCAAAGAG CAGCAGTCAG 
GAGTTCCTTG AGGACAAGGA TAATGTCAAG AAGGGCGGTC TGTGCGGATG CTGCTCCAAG 
CTGTTCATGC CCTGCCGCCG ATCCCGCTGC TCTCGTTGCT GCCGGCGCCG TGAGCGAAAC 
GAATCCGCCG AGGATCAGCC TGTCCTGTGG AGCGGCAACA GTAGTGCCAC CACGGCCACC 
AATGTCCAAG TGATTGACGA GACCAAGCCG AAGCGCAAGA AGATATCGGA TTGGCTAAAG 
TCCAGCTGCT GCCGGAGCTG TCGCAAGAAA CCAGCCATCG AGGAGCACGA AGCACATCAG 
GAGGAAGTGT CCAAGCGGAC GAACCAGGCG GCTAACATGA GCACGGGACC AAGGACACGA 
GGCAAGTGCG GCCTCTGTCT GAGCAAGGTG TTCTGCTGTC GATCGGTGAA CAGGGTGGAT 
CCCACCACCG GCGACGAGAC GGAGATGAAA CAGTGCTGCT TCTGCATACC ATGTCGTCGA 
GGCAGTCGAC CCAACAAGAA GGGCAGCACC GTCTCCTGGC GGGATCAGGA CCCCGAGCTG 
GGCATCAAGC CCACCGAGTC CTCGGTGGTG GAAGGCGCCT CGGAGGCAGC CATGTCAGCG 
GCAACCGATG CAGGGAATGA CCAAGTTAAG GAGGGCTGCT GCAAGCGCTT CTGGCTGATG 
TTGCTCTGCT GTCGCAAGAG GAAGCGCCGC GAGTCCAACG CTAGGAGACA GAGCATTAGG 
GCGCCGCCAC CCAGTGAGGA TACGCGGAAG AAGCTACACG TGGACCTGGT GGAGTACTCG 
TCCAAGATGA AGGGAGCTAT TCCCGTACTG CCGCTGTATT TAGCCTGGTT CTGTGCCTTC 
TGCAATGTGG TTTTCCCAGG ACTAGGAACC CTGCTCTCCG GACTATTTTG CCTCTGCGTG 
GGCATTCCGC GTTTCTCGCA GTTCGACAGC GCTCGTGCAA GGATCGGATC ATTCATCATC 
AACATTATCG TGGCCGTATC GCAGTTCTTC TGCGTGCTCT TCTGCTTCGT GGGATGGGGC 
TGGTCCATTT GGTGGGGCAC CATAATGCTT AGATGTGCAA AGAAATTGAG CAAAATCAAA 
AAGGTGGAGC GTTTGGAGCT GGAGGAGGAG CAACGCCAGG CGCAACTGGC GGAGGCTGGG 
AAAAACGGCG TCGCCGAGGC GGACAAGACA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611603.3
CDS	588..6200
Translation	MQQTLSYSSSSPSPSPMPMPSASAAQTPPPNPNLTPNLNIRPSTNPIPRPRSTGGESAFQPAIIPVDYSYRTHTTRSILSAQSMAPSTFASNQSFVRQPSESDAQPYSSLFGDPVPPPLQPDDYFGTAAFETLRNGSVELPSSEEDALVAAMALNALIPAARERIFPSVPYSRTPRGSLLPYLQPPDIQILPNSSGGSSSEQLGSPNYRLLAPDDILNKPDVYDLENEVEEYTVVGQRMSFDDEEDEPTRTPSQAPAKLSVSSITTEDHRGLLSDSYSGRDWFRPAPQHFPEFTRIPRLRPSSKMIMSMKQDEGPLSPLATMIQDMNSSSSGGEERVAPNQSRPVSRDVVLSPKQYLEEQLGLERGSQRQSLEDYRMPPPPADPPPFRRTDLTEQDDSRSVEPEHSLKREMSPVIIRDSIPMGGDYRVEQPRPHKKTAFTILSTGQAASSAPRPPQQAPTRGRHASIQSTSTTTSSPAGRPQISPRRRSIFGSEPRLPELPSALGYTSHLVHQTTDDSPRPSVQFNMSGRHSRSKLAIPQKGILQQRDSLTSSIDTFTPRAQIRRGSIGRDPIRSLPYGQKIKSMETLPLINDQHRSSIPRLHYGSTMDLSRPEPAVFPPGALPRPLKPNLNPTRRQRGLLKIINKEESLTHNPPRSNWCSLDDLSRPSFDGTPNQARRRSSSTSPERRSSTQTVSDRRSSNLSSVTATTSDFSFRRPTLSTLPAAQTLSRMRRKSVQALSPNRIPNRIPSFGQRRQSIYQRDQEPKPLSRHGKIAKPSTLSPIIGTPNKDSSSAQSPNHRSSLMADAVDTPSEVSTRRDSVSRIPVRKSPDSRSSSPPKDFGIPASGRSSRASITRSPSRTMNAGSRSPSRSMHPSRTSSRESGRPGDSRSASRGPPSRPGSRLDQIGSRMDRPNSRLERSSSRAERSSSRADRPSSRFDVSNSRGNSRANSRLSINSSSLRSSSISPATMARNRSMRATTPSRRKSISLSPASAMMGRRKSISPDAVGQNRRVSSRGKPEPPEILSRRNSRSRIPTRSTKVASKSPPSSSKKRSKSPEKPKAMGSTPKSNKVGKGVTAKGSPKAKPKIAASAKPKTKTESLMKPPVKNANKTPVNGRPTTNAKKPASKAISTKDAKVEKGKETGKSVASKSNGKETESSVKSSKSQVTKTKPPGGTTSEAKKPATTKTHGITLEKGTNGTDPVGGIPPLAAQVGNAVLQAVEAVPAVTSEVTVPSTPGGKGAGGANMSKLVRMSSRMSLLSNKSTKVRSDSPQNRKVATVQEHATEKEETVGASSQPMSNDAAAILEKSQKTLENIQKTVTEATDEIHKTISENLTDLKSLENDLAADPSPTPSSGTTMARPGESASRTGTADGSIAPQSSGELPKSPFPPTQPIEASVSVLHPNESSAERIASVPEVECESSDLPTALDASESELVGNVMPTPESGADFKVDAKRRSPDGQGGSTAGSGGLAKSSSQEFLEDKDNVKKGGLCGCCSKLFMPCRRSRCSRCCRRRERNESAEDQPVLWSGNSSATTATNVQVIDETKPKRKKISDWLKSSCCRSCRKKPAIEEHEAHQEEVSKRTNQAANMSTGPRTRGKCGLCLSKVFCCRSVNRVDPTTGDETEMKQCCFCIPCRRGSRPNKKGSTVSWRDQDPELGIKPTESSVVEGASEAAMSAATDAGNDQVKEGCCKRFWLMLLCCRKRKRRESNARRQSIRAPPPSEDTRKKLHVDLVEYSSKMKGAIPVLPLYLAWFCAFCNVVFPGLGTLLSGLFCLCVGIPRFSQFDSARARIGSFIINIIVAVSQFFCVLFCFVGWGWSIWWGTIMLRCAKKLSKIKKVERLELEEEQRQAQLAEAGKNGVAEADKT

Target ORF information:

RefSeq Version	NM_137759.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster stumble (stum), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137759.5

ORF Insert Sequence:

ATGCAGCAGA CCCTCTCGTA CTCATCATCA TCGCCATCCC CATCCCCGAT GCCCATGCCA 
TCAGCCTCAG CCGCACAGAC GCCGCCACCG AACCCGAATC TGACACCGAA TCTGAACATA 
CGTCCAAGTA CGAACCCAAT ACCACGACCC AGATCCACTG GAGGCGAGTC CGCGTTCCAG 
CCGGCCATCA TCCCGGTTGA TTATAGCTAT CGCACCCATA CCACCCGCTC CATTCTCTCC 
GCCCAATCGA TGGCACCCTC CACGTTCGCC TCCAATCAGA GCTTTGTGCG CCAGCCCTCG 
GAGTCGGATG CCCAGCCGTA TAGTAGCCTC TTTGGGGATC CGGTTCCACC GCCTCTGCAG 
CCGGATGATT ACTTCGGAAC AGCAGCATTT GAGACGTTGC GAAATGGGTC TGTGGAACTG 
CCCAGCTCCG AGGAGGATGC GCTGGTGGCC GCCATGGCGC TGAACGCACT TATTCCTGCG 
GCCAGGGAAC GCATCTTTCC GAGTGTCCCG TACTCGAGGA CTCCCCGAGG ATCCCTACTG 
CCTTATCTCC AGCCACCCGA CATCCAGATT CTGCCCAACT CGAGCGGTGG CTCCAGTTCA 
GAGCAACTGG GCTCACCTAA CTATCGGCTC CTGGCCCCCG ATGACATTCT GAACAAGCCG 
GACGTTTATG ATTTGGAGAA CGAGGTCGAG GAGTACACCG TAGTCGGTCA GAGAATGTCT 
TTCGATGATG AGGAAGATGA GCCGACGAGG ACTCCTAGCC AAGCGCCCGC CAAGTTGAGT 
GTGTCCAGCA TCACGACGGA GGACCACAGG GGATTACTCA GTGACTCCTA TTCGGGAAGG 
GATTGGTTCC GTCCGGCGCC ACAGCACTTT CCAGAGTTCA CACGCATCCC ACGATTGCGG 
CCCAGCTCCA AAATGATTAT GTCGATGAAG CAGGATGAAG GACCTCTCTC CCCACTGGCC 
ACCATGATCC AGGACATGAA CAGCTCGAGC AGCGGCGGAG AGGAGCGAGT GGCACCCAAC 
CAAAGCAGGC CCGTTAGCAG GGACGTTGTA CTCTCACCGA AGCAATATCT GGAGGAACAG 
TTGGGTCTGG AGAGAGGTAG CCAGAGGCAG TCGCTGGAGG ACTATAGAAT GCCACCTCCA 
CCGGCAGACC CGCCCCCTTT CCGTAGAACT GACTTGACGG AGCAGGATGA CAGCCGCAGT 
GTGGAGCCTG AACATAGCTT AAAGCGGGAA ATGTCCCCAG TTATCATCAG GGATTCAATA 
CCTATGGGCG GAGATTATAG AGTGGAGCAA CCTCGTCCAC ACAAAAAGAC TGCTTTCACG 
ATACTTTCCA CGGGACAGGC AGCTTCTTCT GCTCCTCGGC CTCCACAACA AGCTCCCACC 
AGAGGAAGAC ACGCCTCCAT TCAGTCGACA TCGACGACTA CCTCATCGCC GGCCGGCAGG 
CCTCAGATCA GTCCTCGACG GAGATCGATC TTTGGCTCAG AGCCGCGACT GCCTGAGCTG 
CCCTCCGCGC TGGGTTACAC CAGTCACCTC GTCCACCAGA CCACGGATGA CTCGCCACGC 
CCATCGGTTC AGTTCAATAT GAGTGGACGC CACAGTCGCA GCAAGCTGGC CATTCCGCAG 
AAGGGCATTC TCCAGCAGCG GGACTCGCTG ACCTCCTCCA TCGACACCTT CACGCCAAGA 
GCCCAAATAA GAAGGGGTTC CATTGGCAGG GATCCCATCC GCAGCTTGCC CTATGGCCAA 
AAGATCAAGA GCATGGAAAC ACTGCCCCTG ATAAACGATC AGCATCGCAG CTCCATTCCC 
CGCCTGCACT ACGGATCCAC CATGGATCTG AGTAGGCCGG AACCTGCTGT TTTTCCGCCC 
GGAGCACTGC CGCGACCACT GAAGCCAAAT CTGAATCCCA CGCGAAGGCA ACGCGGTCTC 
CTGAAGATCA TTAACAAGGA GGAGTCGCTC ACCCACAACC CGCCGCGTTC CAACTGGTGC 
AGTTTGGATG ACCTGAGCCG ACCCTCCTTC GATGGCACTC CCAACCAGGC AAGACGTCGC 
TCCAGCTCGA CCTCACCGGA GCGTCGCAGT TCCACGCAAA CGGTCTCGGA TCGAAGAAGT 
TCCAACCTGA GCAGCGTCAC CGCCACCACC TCGGATTTCA GTTTCCGCCG ACCAACGCTC 
TCCACACTGC CAGCAGCTCA GACACTGTCC AGAATGCGTC GGAAATCTGT GCAGGCTCTA 
AGTCCGAATA GGATACCCAA CAGGATACCA AGCTTTGGAC AGCGAAGGCA GTCCATCTAT 
CAGAGGGATC AGGAGCCCAA GCCGTTGAGC AGGCACGGAA AGATCGCCAA GCCGAGCACT 
CTGTCACCCA TAATTGGAAC CCCAAATAAG GACAGCAGTT CGGCACAGAG TCCAAACCAC 
AGATCCTCGC TGATGGCCGA TGCGGTGGAT ACCCCATCCG AGGTTTCCAC GCGACGGGAC 
TCGGTGTCCC GCATACCGGT TCGCAAAAGT CCAGACTCAC GCTCCAGTTC GCCACCCAAA 
GACTTTGGAA TCCCTGCCTC TGGACGAAGC TCGCGGGCTA GCATTACTAG GTCCCCTTCC 
AGAACCATGA ATGCCGGCAG TCGATCCCCT TCCAGATCCA TGCATCCCAG CAGAACGTCC 
TCGAGGGAGA GTGGAAGACC TGGAGACTCG AGGTCGGCAT CCAGGGGCCC ACCTTCTAGG 
CCTGGTTCCC GATTGGATCA GATCGGTTCC CGAATGGATC GGCCCAATTC ACGCTTGGAA 
AGATCCAGCT CACGCGCTGA GAGATCTAGT TCCCGGGCGG ATAGGCCCAG TTCCCGTTTC 
GATGTGTCCA ACTCGCGGGG AAACTCACGT GCAAACTCGC GACTGAGCAT CAACTCTTCC 
TCGCTGCGGT CGTCCAGCAT TTCACCCGCT ACCATGGCCA GGAACAGGAG CATGCGTGCC 
ACAACACCCA GTCGCCGAAA GTCCATCTCC TTGAGTCCAG CCAGTGCTAT GATGGGTCGC 
CGGAAATCAA TCAGTCCCGA TGCCGTTGGT CAAAACCGAA GAGTTTCTAG TCGGGGTAAG 
CCCGAGCCTC CAGAGATTCT ATCTCGAAGG AACTCCCGCT CGCGCATTCC CACCAGATCG 
ACTAAAGTTG CCTCCAAATC CCCGCCGTCT TCGTCCAAGA AGAGATCGAA ATCGCCGGAG 
AAACCCAAAG CGATGGGATC TACGCCCAAG AGCAACAAAG TTGGAAAAGG GGTAACCGCT 
AAAGGAAGCC CCAAAGCGAA GCCGAAGATC GCTGCAAGTG CCAAACCAAA GACCAAGACG 
GAATCCTTGA TGAAACCACC AGTGAAGAAT GCAAACAAAA CTCCTGTAAA TGGTAGACCA 
ACCACAAATG CAAAAAAGCC AGCCAGCAAG GCGATTTCCA CAAAGGATGC AAAAGTGGAG 
AAGGGAAAGG AGACTGGGAA GTCTGTGGCC TCCAAGAGCA ATGGTAAAGA AACCGAGTCT 
TCAGTAAAAT CCAGTAAATC CCAAGTCACA AAAACTAAAC CACCTGGGGG AACAACATCG 
GAGGCCAAGA AACCAGCCAC CACAAAAACC CATGGCATAA CTTTAGAGAA AGGGACAAAT 
GGAACCGATC CCGTTGGAGG AATTCCTCCT TTGGCGGCTC AGGTTGGCAA TGCAGTTCTG 
CAGGCGGTGG AAGCGGTTCC TGCGGTTACC AGTGAGGTTA CCGTGCCCTC AACGCCAGGA 
GGTAAAGGTG CTGGCGGCGC CAATATGTCG AAACTGGTGC GAATGAGCTC CAGGATGAGC 
CTGCTGAGCA ACAAGAGCAC CAAGGTGCGA TCAGATTCTC CTCAAAACCG AAAGGTGGCC 
ACCGTACAGG AACATGCTAC AGAAAAAGAA GAAACAGTGG GAGCCAGCTC GCAACCCATG 
TCCAATGATG CTGCTGCTAT TCTGGAAAAG TCGCAGAAAA CTCTGGAGAA CATTCAAAAG 
ACCGTAACTG AGGCCACCGA CGAGATCCAT AAGACCATCA GCGAAAATCT CACCGATCTG 
AAAAGTCTGG AGAACGACCT TGCCGCTGAT CCCTCGCCAA CGCCCAGTTC GGGTACCACG 
ATGGCCAGGC CGGGTGAATC CGCCTCCCGG ACGGGCACAG CCGACGGCAG CATTGCTCCT 
CAGAGTTCGG GGGAGCTGCC CAAGTCACCG TTTCCACCCA CCCAACCCAT CGAAGCCTCT 
GTCTCGGTTT TGCATCCCAA TGAAAGCTCC GCCGAGCGAA TAGCCAGCGT GCCCGAGGTG 
GAGTGCGAAA GCTCTGATCT TCCAACGGCC CTAGACGCTT CCGAATCCGA GCTGGTTGGT 
AATGTTATGC CCACGCCCGA GAGTGGAGCG GACTTCAAAG TGGACGCCAA GCGGCGATCG 
CCGGACGGAC AGGGAGGATC CACGGCGGGA AGCGGCGGTC TGGCAAAGAG CAGCAGTCAG 
GAGTTCCTTG AGGACAAGGA TAATGTCAAG AAGGGCGGTC TGTGCGGATG CTGCTCCAAG 
CTGTTCATGC CCTGCCGCCG ATCCCGCTGC TCTCGTTGCT GCCGGCGCCG TGAGCGAAAC 
GAATCCGCCG AGGATCAGCC TGTCCTGTGG AGCGGCAACA GTAGTGCCAC CACGGCCACC 
AATGTCCAAG TGATTGACGA GACCAAGCCG AAGCGCAAGA AGATATCGGA TTGGCTAAAG 
TCCAGCTGCT GCCGGAGCTG TCGCAAGAAA CCAGCCATCG AGGAGCACGA AGCACATCAG 
GAGGAAGTGT CCAAGCGGAC GAACCAGGCG GCTAACATGA GCACGGGACC AAGGACACGA 
GGCAAGTGCG GCCTCTGTCT GAGCAAGGTG TTCTGCTGTC GATCGGTGAA CAGGGTGGAT 
CCCACCACCG GCGACGAGAC GGAGATGAAA CAGTGCTGCT TCTGCATACC ATGTCGTCGA 
GGCAGTCGAC CCAACAAGAA GGGCAGCACC GTCTCCTGGC GGGATCAGGA CCCCGAGCTG 
GGCATCAAGC CCACCGAGTC CTCGGTGGTG GAAGGCGCCT CGGAGGCAGC CATGTCAGCG 
GCAACCGATG CAGGGAATGA CCAAGTTAAG GAGGGCTGCT GCAAGCGCTT CTGGCTGATG 
TTGCTCTGCT GTCGCAAGAG GAAGCGCCGC GAGTCCAACG CTAGGAGACA GAGCATTAGG 
GCGCCGCCAC CCAGTGAGGA TACGCGGAAG AAGCTACACG TGGACCTGGT GGAGTACTCG 
TCCAAGATGA AGGGAGCTAT TCCCGTACTG CCGCTGTATT TAGCCTGGTT CTGTGCCTTC 
TGCAATGTGG TTTTCCCAGG ACTAGGAACC CTGCTCTCCG GACTATTTTG CCTCTGCGTG 
GGCATTCCGC GTTTCTCGCA GTTCGACAGC GCTCGTGCAA GGATCGGATC ATTCATCATC 
AACATTATCG TGGCCGTATC GCAGTTCTTC TGCGTGCTCT TCTGCTTCGT GGGATGGGGC 
TGGTCCATTT GGTGGGGCAC CATAATGCTT AGATGTGCAA AGAAATTGAG CAAAATCAAA 
AAGGTGGAGC GTTTGGAGCT GGAGGAGGAG CAACGCCAGG CGCAACTGGC GGAGGCTGGG 
AAAAACGGCG TCGCCGAGGC GGACAAGACA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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stum cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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