Egfr cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Egfr
Entrez Gene ID	37455
Full Name	Epidermal growth factor receptor
Synonyms	C-erb,CG10079,D-EGFR,D-Egf,DEGFR,DER,DER flb,DER/EGFR,DER/top,DER/torpedo,DER1,DEgfr,Degfr,Der,DmHD-33,Dmel\CG10079,EC2-4,EFG-R,EGF-R,EGFR,EGFr,EGfr,EK2-6,Egf,Egf-r,EgfR,El,Elp,Elp-1,Elp-B1,Elp-B1RB1,Flb,HD-33,TOP,Tor,Torpedo/DER,Torpedo/Egfr,c-erbB,d-egf-r,dEGFR,dEGFR1,dEGFr,dEgfr,degfr,der,egfr,flb,l(2)05351,l(2)09261,l(2)57DEFa,l(2)57EFa,l(2)57Ea,mor1,top,top/DER,top/flb,torpedo/Egfr,torpedo/egfr
General protein information	Preferred Names Epidermal growth factor receptor Names CG10079 gene product from transcript CG10079-RA CG10079-PA CG10079-PB DER-Ellipse EGF-receptor EGFR Egf receptor Egfr-PA Egfr-PB dEGFR drosophila epidermal growth factor receptor homologue ellipse ellipse torpedo epidermal growth factor receptor faint little ball morphological defects 1 torpedo torpedo/DER
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 57E9-57F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_057411.4	NP_476759.1	epidermal growth factor receptor, isoform B
NM_057410.4	NP_476758.1	epidermal growth factor receptor, isoform A

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	EGFR	XP_314949.3
Danio rerio (zebrafish)	erbb3a	NP_001005320.1
Danio rerio (zebrafish)	erbb3b	NP_001014826.1
Drosophila melanogaster (fruit fly)	Egfr	NP_476758.1
Caenorhabditis elegans (roundworm)	let-23	NP_001129830.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Graf functions to downregulate EGFR signaling.
Title: Graf regulates hematopoiesis through GEEC endocytosis of EGFR.

we find that EGFR regulates the apical determinant Crb and the extracellular matrix regulator Serp, two factors previously known to control tube length. EGFR regulates the organisation of endosomes in which Crb and Serp proteins are loaded
Title: EGFR controls Drosophila tracheal tube elongation by intracellular trafficking regulation.

Here we uncover a cell non-autonomous requirement for the Epidermal growth factor receptor (Egfr) pathway in the lateral epidermis for sustained dpp expression in the LE. Specifically, we demonstrate that Egfr pathway activity in the lateral epidermis prevents expression of the gene scarface (scaf), encoding a secreted antagonist of JNK signaling
Title: Novel interplay between JNK and Egfr signaling in Drosophila dorsal closure.

Loss of Usp5 results in upregulation of Notch signaling and downregulation of RTK signaling by EGF receptor (EGFR) and Sevenless (Sev), leading to impaired photoreceptor development.
Title: The deubiquitinating enzyme Usp5 regulates Notch and RTK signaling during Drosophila eye development.

Data show that EGFR controls the proper formation of brain neuroblasts by regulating the number, survival and proneural gene expression of neuroectodermal progenitor cells which suggest that EGFR signalling is crucially important for patterning and early neurogenesis of the brain.
Title: Genetic regulation and function of epidermal growth factor receptor signalling in patterning of the embryonic Drosophila brain.

The following Egfr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Egfr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa10820	NM_057410.4 Latest version!	Drosophila melanogaster epidermal growth factor receptor, transcript variant A (Egfr), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa10821	NM_057411.4 Latest version!	Drosophila melanogaster epidermal growth factor receptor, transcript variant B (Egfr), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Egfr ( NM_057410.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476758.1 Drosophila melanogaster epidermal growth factor receptor, transcript variant A (Egfr), mRNA.

Price & Availability↑

CloneID	OFa10820
Clone ID Related Accession (Same CDS sequence)	NM_057410.4
Accession Version	NM_057410.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	epidermal growth factor receptor, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_057410.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGATTA TCAGCATGTG GATGAGCATA TCGCGAGGAT TGTGGGACAG CAGCTCCATC 
TGGTCGGTGC TGCTGATCCT CGCCTGCATG GCATCCATCA CCACAAGCTC ATCGGTCAGC 
AATGCCGGCT ATGTGGATAA TGGCAATATG AAAGTCTGCA TCGGCACTAA ATCTCGGCTC 
TCCGTGCCCT CCAACAAGGA ACATCATTAC CGGAACCTCA GAGATCGGTA CACGAACTGT 
ACGTATGTGG ATGGCAACCT GGAGCTGACC TGGCTGCCCA ACGAGAATTT GGACCTCAGC 
TTCCTGGACA ACATACGGGA GGTCACCGGC TATATTCTGA TCAGTCATGT GGACGTTAAG 
AAAGTGGTAT TTCCCAAACT ACAAATCATT CGCGGACGCA CGCTGTTCAG CTTATCCGTG 
GAGGAGGAGA AGTATGCCTT GTTCGTCACT TATTCCAAAA TGTACACGCT GGAGATTCCC 
GATCTACGCG ATGTCTTAAA TGGCCAAGTG GGCTTCCACA ACAACTACAA TCTCTGCCAC 
ATGCGAACGA TCCAGTGGTC GGAGATTGTA TCCAACGGCA CGGATGCATA CTACAACTAC 
GACTTTACTG CTCCGGAGCG CGAGTGTCCC AAGTGCCACG AGAGCTGCAC GCACGGATGT 
TGGGGCGAGG GTCCCAAGAA TTGCCAGAAG TTCAGCAAGC TCACCTGCTC GCCACAGTGT 
GCCGGAGGTC GTTGCTATGG ACCAAAGCCG CGGGAGTGTT GTCACCTCTT CTGCGCCGGA 
GGATGCACTG GTCCCACGCA AAAGGATTGC ATCGCCTGCA AGAACTTCTT CGACGAGGGC 
GTATGCAAGG AGGAATGCCC GCCCATGCGC AAGTACAATC CCACCACCTA TGTTCTTGAA 
ACGAATCCTG AGGGAAAGTA TGCCTATGGT GCCACCTGCG TCAAGGAGTG TCCCGGTCAT 
CTGTTGCGTG ATAATGGCGC CTGCGTGCGC AGCTGTCCCC AGGACAAGAT GGACAAGGGG 
GGCGAGTGTG TGCCCTGCAA TGGACCGTGC CCCAAAACCT GCCCGGGCGT TACTGTCCTG 
CATGCCGGCA ACATTGACTC GTTCCGGAAT TGTACGGTGA TCGATGGCAA CATTCGCATT 
TTGGATCAGA CCTTCTCGGG CTTCCAGGAT GTCTATGCCA ACTACACGAT GGGACCACGA 
TACATACCGC TGGATCCCGA GCGACTGGAG GTGTTCTCCA CGGTGAAGGA GATCACCGGG 
TATCTGAATA TCGAGGGAAC CCACCCGCAG TTCCGGAATC TGTCGTACTT CCGCAATCTG 
GAAACAATTC ATGGCCGCCA GCTGATGGAG AGCATGTTTG CCGCTTTGGC GATCGTTAAG 
TCATCCCTGT ACAGCCTGGA GATGCGCAAT CTGAAGCAGA TTAGTTCCGG CAGTGTGGTC 
ATCCAGCATA ATAGAGACCT CTGCTACGTA AGCAATATCC GTTGGCCGGC CATTCAGAAG 
GAGCCCGAAC AGAAGGTGTG GGTCAACGAG AATCTCAGGG CGGATCTATG CGAGAAAAAT 
GGAACCATTT GCTCGGATCA GTGCAACGAG GACGGCTGCT GGGGAGCTGG CACGGATCAG 
TGCCTTACCT GCAAGAACTT CAATTTCAAT GGCACCTGCA TCGCCGACTG TGGTTATATA 
TCCAATGCCT ACAAGTTTGA CAATAGAACG TGCAAGATAT GCCATCCAGA GTGCCGGACT 
TGCAATGGAG CTGGAGCAGA TCACTGCCAG GAGTGCGTCC ATGTGAGGGA CGGTCAGCAC 
TGTGTGTCCG AGTGCCCGAA GAACAAGTAC AACGATCGTG GTGTCTGCCG AGAGTGCCAC 
GCCACCTGCG ATGGATGCAC TGGGCCCAAG GACACCATCG GCATTGGAGC GTGTACAACG 
TGCAATTTGG CCATTATCAA CAATGACGCC ACAGTAAAAC GCTGCCTGCT GAAGGACGAC 
AAGTGCCCCG ATGGGTACTT CTGGGAGTAT GTGCATCCAC AAGAGCAGGG ATCGCTAAAG 
CCATTGGCCG GCAGAGCAGT TTGCCGAAAG TGCCATCCCC TTTGCGAGCT GTGCACCAAC 
TACGGATACC ATGAACAGGT GTGCTCCAAG TGCACCCACT ACAAGCGACG AGAGCAGTGC 
GAGACCGAGT GTCCGGCCGA TCACTACACG GATGAGGAGC AGCGCGAGTG CTTCCAGTGC 
CACCCAGAAT GCAACGGTTG CACTGGTCCG GGTGCCGACG ATTGCAAGTC TTGTCGCAAC 
TTCAAGTTGT TCGACGCGAA TGAGACGGGT CCCTATGTGA ACTCCACGAT GTTCAATTGC 
ACCTCGAAGT GTCCCTTGGA GATGCGACAT GTGAACTATC AGTACACGGC CATTGGACCC 
TACTGTGCAG CTAGTCCGCC GAGGAGCAGC AAGATAACTG CCAATCTGGA TGTGAACATG 
ATCTTCATTA TCACTGGTGC TGTTCTGGTG CCGACGATCT GCATCCTCTG CGTGGTCACA 
TACATTTGTC GGCAAAAGCA AAAGGCCAAG AAAGAAACAG TGAAGATGAC CATGGCTCTG 
TCCGGCTGTG AGGATTCCGA GCCGCTGCGT CCCTCGAACA TTGGAGCCAA TCTATGCAAG 
TTGCGCATTG TCAAGGACGC CGAGTTGCGC AAGGGCGGAG TCCTCGGAAT GGGAGCCTTT 
GGACGAGTGT ACAAGGGCGT TTGGGTGCCG GAGGGTGAGA ACGTCAAGAT TCCAGTGGCC 
ATTAAGGAGC TGCTCAAGTC CACAGGCGCC GAGTCAAGCG AAGAGTTCCT CCGCGAAGCC 
TACATCATGG CCTCTGTGGA GCACGTTAAT CTGCTGAAGC TCCTGGCCGT CTGCATGTCC 
TCACAAATGA TGCTAATCAC GCAACTGATG CCGCTTGGCT GCCTGTTGGA CTATGTGCGA 
AATAACCGGG ACAAGATCGG CTCTAAGGCT CTGCTCAACT GGAGCACGCA AATCGCCAAG 
GGCATGTCGT ATCTGGAGGA GAAGCGACTG GTCCACAGAG ACTTGGCTGC CCGCAATGTC 
CTGGTGCAGA CTCCCTCGCT GGTGAAGATC ACCGACTTTG GGCTGGCCAA GTTGCTGAGC 
AGCGATTCCA ATGAGTACAA GGCTGCTGGC GGCAAGATGC CCATCAAGTG GTTGGCACTG 
GAGTGCATTC GCAATCGTGT ATTCACCAGC AAGTCCGATG TCTGGGCCTT TGGTGTGACA 
ATTTGGGAAC TGCTGACCTT TGGCCAGCGT CCACACGAGA ACATCCCCGC TAAGGATATT 
CCCGATCTTA TTGAAGTCGG TCTGAAGCTG GAGCAGCCGG AGATTTGTTC GCTGGACATT 
TACTGCACAC TTCTCTCGTG CTGGCACTTG GATGCCGCCA TGCGTCCAAC CTTCAAGCAG 
CTGACTACGG TCTTTGCTGA GTTCGCCAGA GATCCGGGTC GCTATCTGGC CATTCCCGGG 
GATAAGTTCA CCCGGCTGCC GGCCTACACG AGTCAGGATG AGAAGGATCT CATCCGAAAA 
TTGGCTCCCA CCACCGATGG GTCCGAAGCC ATTGCGGAAC CCGATGACTA CCTGCAACCC 
AAGGCAGCAC CTGGTCCTAG TCACAGAACC GACTGCACGG ATGAGATACC CAAGCTGAAC 
CGCTACTGCA AGGATCCTAG CAACAAGAAT TCGAGTACCG GAGACGATGA GACGGATTCG 
AGTGCCCGGG AAGTGGGCGT GGGTAATCTG CGCCTCGATC TACCAGTCGA TGAGGATGAT 
TACCTGATGC CCACATGCCA ACCGGGGCCC AACAACAACA ACAACATAAA TAATCCCAAT 
CAAAACAATA TGGCAGCTGT GGGCGTGGCT GCCGGCTACA TGGATCTCAT CGGAGTGCCC 
GTTAGTGTGG ACAATCCGGA GTATCTGCTA AACGCGCAGA CACTGGGTGT TGGGGAGTCG 
CCGATACCCA CCCAGACCAT CGGGATACCG GTGATGGGAG TCCCGGGCAC CATGGAGGTC 
AAGGTGCCAA TGCCAGGCAG TGAGCCAACG AGCTCCGATC ACGAGTACTA CAATGATACC 
CAACGGGAGT TGCAGCCACT GCATCGAAAC CGCAACACGG AGACGAGGGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476758.1
CDS	670..4803
Translation	MMIISMWMSISRGLWDSSSIWSVLLILACMASITTSSSVSNAGYVDNGNMKVCIGTKSRLSVPSNKEHHYRNLRDRYTNCTYVDGNLELTWLPNENLDLSFLDNIREVTGYILISHVDVKKVVFPKLQIIRGRTLFSLSVEEEKYALFVTYSKMYTLEIPDLRDVLNGQVGFHNNYNLCHMRTIQWSEIVSNGTDAYYNYDFTAPERECPKCHESCTHGCWGEGPKNCQKFSKLTCSPQCAGGRCYGPKPRECCHLFCAGGCTGPTQKDCIACKNFFDEGVCKEECPPMRKYNPTTYVLETNPEGKYAYGATCVKECPGHLLRDNGACVRSCPQDKMDKGGECVPCNGPCPKTCPGVTVLHAGNIDSFRNCTVIDGNIRILDQTFSGFQDVYANYTMGPRYIPLDPERLEVFSTVKEITGYLNIEGTHPQFRNLSYFRNLETIHGRQLMESMFAALAIVKSSLYSLEMRNLKQISSGSVVIQHNRDLCYVSNIRWPAIQKEPEQKVWVNENLRADLCEKNGTICSDQCNEDGCWGAGTDQCLTCKNFNFNGTCIADCGYISNAYKFDNRTCKICHPECRTCNGAGADHCQECVHVRDGQHCVSECPKNKYNDRGVCRECHATCDGCTGPKDTIGIGACTTCNLAIINNDATVKRCLLKDDKCPDGYFWEYVHPQEQGSLKPLAGRAVCRKCHPLCELCTNYGYHEQVCSKCTHYKRREQCETECPADHYTDEEQRECFQCHPECNGCTGPGADDCKSCRNFKLFDANETGPYVNSTMFNCTSKCPLEMRHVNYQYTAIGPYCAASPPRSSKITANLDVNMIFIITGAVLVPTICILCVVTYICRQKQKAKKETVKMTMALSGCEDSEPLRPSNIGANLCKLRIVKDAELRKGGVLGMGAFGRVYKGVWVPEGENVKIPVAIKELLKSTGAESSEEFLREAYIMASVEHVNLLKLLAVCMSSQMMLITQLMPLGCLLDYVRNNRDKIGSKALLNWSTQIAKGMSYLEEKRLVHRDLAARNVLVQTPSLVKITDFGLAKLLSSDSNEYKAAGGKMPIKWLALECIRNRVFTSKSDVWAFGVTIWELLTFGQRPHENIPAKDIPDLIEVGLKLEQPEICSLDIYCTLLSCWHLDAAMRPTFKQLTTVFAEFARDPGRYLAIPGDKFTRLPAYTSQDEKDLIRKLAPTTDGSEAIAEPDDYLQPKAAPGPSHRTDCTDEIPKLNRYCKDPSNKNSSTGDDETDSSAREVGVGNLRLDLPVDEDDYLMPTCQPGPNNNNNINNPNQNNMAAVGVAAGYMDLIGVPVSVDNPEYLLNAQTLGVGESPIPTQTIGIPVMGVPGTMEVKVPMPGSEPTSSDHEYYNDTQRELQPLHRNRNTETRV

Target ORF information:

RefSeq Version	NM_057410.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster epidermal growth factor receptor, transcript variant A (Egfr), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057410.4

ORF Insert Sequence:

ATGATGATTA TCAGCATGTG GATGAGCATA TCGCGAGGAT TGTGGGACAG CAGCTCCATC 
TGGTCGGTGC TGCTGATCCT CGCCTGCATG GCATCCATCA CCACAAGCTC ATCGGTCAGC 
AATGCCGGCT ATGTGGATAA TGGCAATATG AAAGTCTGCA TCGGCACTAA ATCTCGGCTC 
TCCGTGCCCT CCAACAAGGA ACATCATTAC CGGAACCTCA GAGATCGGTA CACGAACTGT 
ACGTATGTGG ATGGCAACCT GGAGCTGACC TGGCTGCCCA ACGAGAATTT GGACCTCAGC 
TTCCTGGACA ACATACGGGA GGTCACCGGC TATATTCTGA TCAGTCATGT GGACGTTAAG 
AAAGTGGTAT TTCCCAAACT ACAAATCATT CGCGGACGCA CGCTGTTCAG CTTATCCGTG 
GAGGAGGAGA AGTATGCCTT GTTCGTCACT TATTCCAAAA TGTACACGCT GGAGATTCCC 
GATCTACGCG ATGTCTTAAA TGGCCAAGTG GGCTTCCACA ACAACTACAA TCTCTGCCAC 
ATGCGAACGA TCCAGTGGTC GGAGATTGTA TCCAACGGCA CGGATGCATA CTACAACTAC 
GACTTTACTG CTCCGGAGCG CGAGTGTCCC AAGTGCCACG AGAGCTGCAC GCACGGATGT 
TGGGGCGAGG GTCCCAAGAA TTGCCAGAAG TTCAGCAAGC TCACCTGCTC GCCACAGTGT 
GCCGGAGGTC GTTGCTATGG ACCAAAGCCG CGGGAGTGTT GTCACCTCTT CTGCGCCGGA 
GGATGCACTG GTCCCACGCA AAAGGATTGC ATCGCCTGCA AGAACTTCTT CGACGAGGGC 
GTATGCAAGG AGGAATGCCC GCCCATGCGC AAGTACAATC CCACCACCTA TGTTCTTGAA 
ACGAATCCTG AGGGAAAGTA TGCCTATGGT GCCACCTGCG TCAAGGAGTG TCCCGGTCAT 
CTGTTGCGTG ATAATGGCGC CTGCGTGCGC AGCTGTCCCC AGGACAAGAT GGACAAGGGG 
GGCGAGTGTG TGCCCTGCAA TGGACCGTGC CCCAAAACCT GCCCGGGCGT TACTGTCCTG 
CATGCCGGCA ACATTGACTC GTTCCGGAAT TGTACGGTGA TCGATGGCAA CATTCGCATT 
TTGGATCAGA CCTTCTCGGG CTTCCAGGAT GTCTATGCCA ACTACACGAT GGGACCACGA 
TACATACCGC TGGATCCCGA GCGACTGGAG GTGTTCTCCA CGGTGAAGGA GATCACCGGG 
TATCTGAATA TCGAGGGAAC CCACCCGCAG TTCCGGAATC TGTCGTACTT CCGCAATCTG 
GAAACAATTC ATGGCCGCCA GCTGATGGAG AGCATGTTTG CCGCTTTGGC GATCGTTAAG 
TCATCCCTGT ACAGCCTGGA GATGCGCAAT CTGAAGCAGA TTAGTTCCGG CAGTGTGGTC 
ATCCAGCATA ATAGAGACCT CTGCTACGTA AGCAATATCC GTTGGCCGGC CATTCAGAAG 
GAGCCCGAAC AGAAGGTGTG GGTCAACGAG AATCTCAGGG CGGATCTATG CGAGAAAAAT 
GGAACCATTT GCTCGGATCA GTGCAACGAG GACGGCTGCT GGGGAGCTGG CACGGATCAG 
TGCCTTACCT GCAAGAACTT CAATTTCAAT GGCACCTGCA TCGCCGACTG TGGTTATATA 
TCCAATGCCT ACAAGTTTGA CAATAGAACG TGCAAGATAT GCCATCCAGA GTGCCGGACT 
TGCAATGGAG CTGGAGCAGA TCACTGCCAG GAGTGCGTCC ATGTGAGGGA CGGTCAGCAC 
TGTGTGTCCG AGTGCCCGAA GAACAAGTAC AACGATCGTG GTGTCTGCCG AGAGTGCCAC 
GCCACCTGCG ATGGATGCAC TGGGCCCAAG GACACCATCG GCATTGGAGC GTGTACAACG 
TGCAATTTGG CCATTATCAA CAATGACGCC ACAGTAAAAC GCTGCCTGCT GAAGGACGAC 
AAGTGCCCCG ATGGGTACTT CTGGGAGTAT GTGCATCCAC AAGAGCAGGG ATCGCTAAAG 
CCATTGGCCG GCAGAGCAGT TTGCCGAAAG TGCCATCCCC TTTGCGAGCT GTGCACCAAC 
TACGGATACC ATGAACAGGT GTGCTCCAAG TGCACCCACT ACAAGCGACG AGAGCAGTGC 
GAGACCGAGT GTCCGGCCGA TCACTACACG GATGAGGAGC AGCGCGAGTG CTTCCAGTGC 
CACCCAGAAT GCAACGGTTG CACTGGTCCG GGTGCCGACG ATTGCAAGTC TTGTCGCAAC 
TTCAAGTTGT TCGACGCGAA TGAGACGGGT CCCTATGTGA ACTCCACGAT GTTCAATTGC 
ACCTCGAAGT GTCCCTTGGA GATGCGACAT GTGAACTATC AGTACACGGC CATTGGACCC 
TACTGTGCAG CTAGTCCGCC GAGGAGCAGC AAGATAACTG CCAATCTGGA TGTGAACATG 
ATCTTCATTA TCACTGGTGC TGTTCTGGTG CCGACGATCT GCATCCTCTG CGTGGTCACA 
TACATTTGTC GGCAAAAGCA AAAGGCCAAG AAAGAAACAG TGAAGATGAC CATGGCTCTG 
TCCGGCTGTG AGGATTCCGA GCCGCTGCGT CCCTCGAACA TTGGAGCCAA TCTATGCAAG 
TTGCGCATTG TCAAGGACGC CGAGTTGCGC AAGGGCGGAG TCCTCGGAAT GGGAGCCTTT 
GGACGAGTGT ACAAGGGCGT TTGGGTGCCG GAGGGTGAGA ACGTCAAGAT TCCAGTGGCC 
ATTAAGGAGC TGCTCAAGTC CACAGGCGCC GAGTCAAGCG AAGAGTTCCT CCGCGAAGCC 
TACATCATGG CCTCTGTGGA GCACGTTAAT CTGCTGAAGC TCCTGGCCGT CTGCATGTCC 
TCACAAATGA TGCTAATCAC GCAACTGATG CCGCTTGGCT GCCTGTTGGA CTATGTGCGA 
AATAACCGGG ACAAGATCGG CTCTAAGGCT CTGCTCAACT GGAGCACGCA AATCGCCAAG 
GGCATGTCGT ATCTGGAGGA GAAGCGACTG GTCCACAGAG ACTTGGCTGC CCGCAATGTC 
CTGGTGCAGA CTCCCTCGCT GGTGAAGATC ACCGACTTTG GGCTGGCCAA GTTGCTGAGC 
AGCGATTCCA ATGAGTACAA GGCTGCTGGC GGCAAGATGC CCATCAAGTG GTTGGCACTG 
GAGTGCATTC GCAATCGTGT ATTCACCAGC AAGTCCGATG TCTGGGCCTT TGGTGTGACA 
ATTTGGGAAC TGCTGACCTT TGGCCAGCGT CCACACGAGA ACATCCCCGC TAAGGATATT 
CCCGATCTTA TTGAAGTCGG TCTGAAGCTG GAGCAGCCGG AGATTTGTTC GCTGGACATT 
TACTGCACAC TTCTCTCGTG CTGGCACTTG GATGCCGCCA TGCGTCCAAC CTTCAAGCAG 
CTGACTACGG TCTTTGCTGA GTTCGCCAGA GATCCGGGTC GCTATCTGGC CATTCCCGGG 
GATAAGTTCA CCCGGCTGCC GGCCTACACG AGTCAGGATG AGAAGGATCT CATCCGAAAA 
TTGGCTCCCA CCACCGATGG GTCCGAAGCC ATTGCGGAAC CCGATGACTA CCTGCAACCC 
AAGGCAGCAC CTGGTCCTAG TCACAGAACC GACTGCACGG ATGAGATACC CAAGCTGAAC 
CGCTACTGCA AGGATCCTAG CAACAAGAAT TCGAGTACCG GAGACGATGA GACGGATTCG 
AGTGCCCGGG AAGTGGGCGT GGGTAATCTG CGCCTCGATC TACCAGTCGA TGAGGATGAT 
TACCTGATGC CCACATGCCA ACCGGGGCCC AACAACAACA ACAACATAAA TAATCCCAAT 
CAAAACAATA TGGCAGCTGT GGGCGTGGCT GCCGGCTACA TGGATCTCAT CGGAGTGCCC 
GTTAGTGTGG ACAATCCGGA GTATCTGCTA AACGCGCAGA CACTGGGTGT TGGGGAGTCG 
CCGATACCCA CCCAGACCAT CGGGATACCG GTGATGGGAG TCCCGGGCAC CATGGAGGTC 
AAGGTGCCAA TGCCAGGCAG TGAGCCAACG AGCTCCGATC ACGAGTACTA CAATGATACC 
CAACGGGAGT TGCAGCCACT GCATCGAAAC CGCAACACGG AGACGAGGGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Egfr ( NM_057411.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476759.1 Drosophila melanogaster epidermal growth factor receptor, transcript variant B (Egfr), mRNA.

Price & Availability↑

CloneID	OFa10821
Clone ID Related Accession (Same CDS sequence)	NM_057411.4
Accession Version	NM_057411.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	epidermal growth factor receptor, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_057411.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGCTGC GACGGCGCAA CGGCCCCTGC CCCTTCCCCC TGCTGCTCCT GCTCCTGGCC 
CACTGCATTT GCATTTGGCC CGCGTCGGCG GCCCGCGATC GCTACGCCCG CCAGAACAAT 
CGCCAGCGCC ATCAGGATAT AGATCGCGAT CGGGATCGAG ATCGATTCCT ATACCGCAGC 
AGTTCGGCCC AAAATCGACA GAGGGGCGGG GCCAACTTCG CCCTGGGACT GGGAGCCAAC 
GGAGTCACCA TTCCCACCAG TCTGGAGGAT AAGAACAAGA ACGAGTTCGT CAAGGGGAAA 
ATCTGCATCG GCACTAAATC TCGGCTCTCC GTGCCCTCCA ACAAGGAACA TCATTACCGG 
AACCTCAGAG ATCGGTACAC GAACTGTACG TATGTGGATG GCAACCTGGA GCTGACCTGG 
CTGCCCAACG AGAATTTGGA CCTCAGCTTC CTGGACAACA TACGGGAGGT CACCGGCTAT 
ATTCTGATCA GTCATGTGGA CGTTAAGAAA GTGGTATTTC CCAAACTACA AATCATTCGC 
GGACGCACGC TGTTCAGCTT ATCCGTGGAG GAGGAGAAGT ATGCCTTGTT CGTCACTTAT 
TCCAAAATGT ACACGCTGGA GATTCCCGAT CTACGCGATG TCTTAAATGG CCAAGTGGGC 
TTCCACAACA ACTACAATCT CTGCCACATG CGAACGATCC AGTGGTCGGA GATTGTATCC 
AACGGCACGG ATGCATACTA CAACTACGAC TTTACTGCTC CGGAGCGCGA GTGTCCCAAG 
TGCCACGAGA GCTGCACGCA CGGATGTTGG GGCGAGGGTC CCAAGAATTG CCAGAAGTTC 
AGCAAGCTCA CCTGCTCGCC ACAGTGTGCC GGAGGTCGTT GCTATGGACC AAAGCCGCGG 
GAGTGTTGTC ACCTCTTCTG CGCCGGAGGA TGCACTGGTC CCACGCAAAA GGATTGCATC 
GCCTGCAAGA ACTTCTTCGA CGAGGGCGTA TGCAAGGAGG AATGCCCGCC CATGCGCAAG 
TACAATCCCA CCACCTATGT TCTTGAAACG AATCCTGAGG GAAAGTATGC CTATGGTGCC 
ACCTGCGTCA AGGAGTGTCC CGGTCATCTG TTGCGTGATA ATGGCGCCTG CGTGCGCAGC 
TGTCCCCAGG ACAAGATGGA CAAGGGGGGC GAGTGTGTGC CCTGCAATGG ACCGTGCCCC 
AAAACCTGCC CGGGCGTTAC TGTCCTGCAT GCCGGCAACA TTGACTCGTT CCGGAATTGT 
ACGGTGATCG ATGGCAACAT TCGCATTTTG GATCAGACCT TCTCGGGCTT CCAGGATGTC 
TATGCCAACT ACACGATGGG ACCACGATAC ATACCGCTGG ATCCCGAGCG ACTGGAGGTG 
TTCTCCACGG TGAAGGAGAT CACCGGGTAT CTGAATATCG AGGGAACCCA CCCGCAGTTC 
CGGAATCTGT CGTACTTCCG CAATCTGGAA ACAATTCATG GCCGCCAGCT GATGGAGAGC 
ATGTTTGCCG CTTTGGCGAT CGTTAAGTCA TCCCTGTACA GCCTGGAGAT GCGCAATCTG 
AAGCAGATTA GTTCCGGCAG TGTGGTCATC CAGCATAATA GAGACCTCTG CTACGTAAGC 
AATATCCGTT GGCCGGCCAT TCAGAAGGAG CCCGAACAGA AGGTGTGGGT CAACGAGAAT 
CTCAGGGCGG ATCTATGCGA GAAAAATGGA ACCATTTGCT CGGATCAGTG CAACGAGGAC 
GGCTGCTGGG GAGCTGGCAC GGATCAGTGC CTTACCTGCA AGAACTTCAA TTTCAATGGC 
ACCTGCATCG CCGACTGTGG TTATATATCC AATGCCTACA AGTTTGACAA TAGAACGTGC 
AAGATATGCC ATCCAGAGTG CCGGACTTGC AATGGAGCTG GAGCAGATCA CTGCCAGGAG 
TGCGTCCATG TGAGGGACGG TCAGCACTGT GTGTCCGAGT GCCCGAAGAA CAAGTACAAC 
GATCGTGGTG TCTGCCGAGA GTGCCACGCC ACCTGCGATG GATGCACTGG GCCCAAGGAC 
ACCATCGGCA TTGGAGCGTG TACAACGTGC AATTTGGCCA TTATCAACAA TGACGCCACA 
GTAAAACGCT GCCTGCTGAA GGACGACAAG TGCCCCGATG GGTACTTCTG GGAGTATGTG 
CATCCACAAG AGCAGGGATC GCTAAAGCCA TTGGCCGGCA GAGCAGTTTG CCGAAAGTGC 
CATCCCCTTT GCGAGCTGTG CACCAACTAC GGATACCATG AACAGGTGTG CTCCAAGTGC 
ACCCACTACA AGCGACGAGA GCAGTGCGAG ACCGAGTGTC CGGCCGATCA CTACACGGAT 
GAGGAGCAGC GCGAGTGCTT CCAGTGCCAC CCAGAATGCA ACGGTTGCAC TGGTCCGGGT 
GCCGACGATT GCAAGTCTTG TCGCAACTTC AAGTTGTTCG ACGCGAATGA GACGGGTCCC 
TATGTGAACT CCACGATGTT CAATTGCACC TCGAAGTGTC CCTTGGAGAT GCGACATGTG 
AACTATCAGT ACACGGCCAT TGGACCCTAC TGTGCAGCTA GTCCGCCGAG GAGCAGCAAG 
ATAACTGCCA ATCTGGATGT GAACATGATC TTCATTATCA CTGGTGCTGT TCTGGTGCCG 
ACGATCTGCA TCCTCTGCGT GGTCACATAC ATTTGTCGGC AAAAGCAAAA GGCCAAGAAA 
GAAACAGTGA AGATGACCAT GGCTCTGTCC GGCTGTGAGG ATTCCGAGCC GCTGCGTCCC 
TCGAACATTG GAGCCAATCT ATGCAAGTTG CGCATTGTCA AGGACGCCGA GTTGCGCAAG 
GGCGGAGTCC TCGGAATGGG AGCCTTTGGA CGAGTGTACA AGGGCGTTTG GGTGCCGGAG 
GGTGAGAACG TCAAGATTCC AGTGGCCATT AAGGAGCTGC TCAAGTCCAC AGGCGCCGAG 
TCAAGCGAAG AGTTCCTCCG CGAAGCCTAC ATCATGGCCT CTGTGGAGCA CGTTAATCTG 
CTGAAGCTCC TGGCCGTCTG CATGTCCTCA CAAATGATGC TAATCACGCA ACTGATGCCG 
CTTGGCTGCC TGTTGGACTA TGTGCGAAAT AACCGGGACA AGATCGGCTC TAAGGCTCTG 
CTCAACTGGA GCACGCAAAT CGCCAAGGGC ATGTCGTATC TGGAGGAGAA GCGACTGGTC 
CACAGAGACT TGGCTGCCCG CAATGTCCTG GTGCAGACTC CCTCGCTGGT GAAGATCACC 
GACTTTGGGC TGGCCAAGTT GCTGAGCAGC GATTCCAATG AGTACAAGGC TGCTGGCGGC 
AAGATGCCCA TCAAGTGGTT GGCACTGGAG TGCATTCGCA ATCGTGTATT CACCAGCAAG 
TCCGATGTCT GGGCCTTTGG TGTGACAATT TGGGAACTGC TGACCTTTGG CCAGCGTCCA 
CACGAGAACA TCCCCGCTAA GGATATTCCC GATCTTATTG AAGTCGGTCT GAAGCTGGAG 
CAGCCGGAGA TTTGTTCGCT GGACATTTAC TGCACACTTC TCTCGTGCTG GCACTTGGAT 
GCCGCCATGC GTCCAACCTT CAAGCAGCTG ACTACGGTCT TTGCTGAGTT CGCCAGAGAT 
CCGGGTCGCT ATCTGGCCAT TCCCGGGGAT AAGTTCACCC GGCTGCCGGC CTACACGAGT 
CAGGATGAGA AGGATCTCAT CCGAAAATTG GCTCCCACCA CCGATGGGTC CGAAGCCATT 
GCGGAACCCG ATGACTACCT GCAACCCAAG GCAGCACCTG GTCCTAGTCA CAGAACCGAC 
TGCACGGATG AGATACCCAA GCTGAACCGC TACTGCAAGG ATCCTAGCAA CAAGAATTCG 
AGTACCGGAG ACGATGAGAC GGATTCGAGT GCCCGGGAAG TGGGCGTGGG TAATCTGCGC 
CTCGATCTAC CAGTCGATGA GGATGATTAC CTGATGCCCA CATGCCAACC GGGGCCCAAC 
AACAACAACA ACATAAATAA TCCCAATCAA AACAATATGG CAGCTGTGGG CGTGGCTGCC 
GGCTACATGG ATCTCATCGG AGTGCCCGTT AGTGTGGACA ATCCGGAGTA TCTGCTAAAC 
GCGCAGACAC TGGGTGTTGG GGAGTCGCCG ATACCCACCC AGACCATCGG GATACCGGTG 
ATGGGAGTCC CGGGCACCAT GGAGGTCAAG GTGCCAATGC CAGGCAGTGA GCCAACGAGC 
TCCGATCACG AGTACTACAA TGATACCCAA CGGGAGTTGC AGCCACTGCA TCGAAACCGC 
AACACGGAGA CGAGGGTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476759.1
CDS	949..5229
Translation	MLLRRRNGPCPFPLLLLLLAHCICIWPASAARDRYARQNNRQRHQDIDRDRDRDRFLYRSSSAQNRQRGGANFALGLGANGVTIPTSLEDKNKNEFVKGKICIGTKSRLSVPSNKEHHYRNLRDRYTNCTYVDGNLELTWLPNENLDLSFLDNIREVTGYILISHVDVKKVVFPKLQIIRGRTLFSLSVEEEKYALFVTYSKMYTLEIPDLRDVLNGQVGFHNNYNLCHMRTIQWSEIVSNGTDAYYNYDFTAPERECPKCHESCTHGCWGEGPKNCQKFSKLTCSPQCAGGRCYGPKPRECCHLFCAGGCTGPTQKDCIACKNFFDEGVCKEECPPMRKYNPTTYVLETNPEGKYAYGATCVKECPGHLLRDNGACVRSCPQDKMDKGGECVPCNGPCPKTCPGVTVLHAGNIDSFRNCTVIDGNIRILDQTFSGFQDVYANYTMGPRYIPLDPERLEVFSTVKEITGYLNIEGTHPQFRNLSYFRNLETIHGRQLMESMFAALAIVKSSLYSLEMRNLKQISSGSVVIQHNRDLCYVSNIRWPAIQKEPEQKVWVNENLRADLCEKNGTICSDQCNEDGCWGAGTDQCLTCKNFNFNGTCIADCGYISNAYKFDNRTCKICHPECRTCNGAGADHCQECVHVRDGQHCVSECPKNKYNDRGVCRECHATCDGCTGPKDTIGIGACTTCNLAIINNDATVKRCLLKDDKCPDGYFWEYVHPQEQGSLKPLAGRAVCRKCHPLCELCTNYGYHEQVCSKCTHYKRREQCETECPADHYTDEEQRECFQCHPECNGCTGPGADDCKSCRNFKLFDANETGPYVNSTMFNCTSKCPLEMRHVNYQYTAIGPYCAASPPRSSKITANLDVNMIFIITGAVLVPTICILCVVTYICRQKQKAKKETVKMTMALSGCEDSEPLRPSNIGANLCKLRIVKDAELRKGGVLGMGAFGRVYKGVWVPEGENVKIPVAIKELLKSTGAESSEEFLREAYIMASVEHVNLLKLLAVCMSSQMMLITQLMPLGCLLDYVRNNRDKIGSKALLNWSTQIAKGMSYLEEKRLVHRDLAARNVLVQTPSLVKITDFGLAKLLSSDSNEYKAAGGKMPIKWLALECIRNRVFTSKSDVWAFGVTIWELLTFGQRPHENIPAKDIPDLIEVGLKLEQPEICSLDIYCTLLSCWHLDAAMRPTFKQLTTVFAEFARDPGRYLAIPGDKFTRLPAYTSQDEKDLIRKLAPTTDGSEAIAEPDDYLQPKAAPGPSHRTDCTDEIPKLNRYCKDPSNKNSSTGDDETDSSAREVGVGNLRLDLPVDEDDYLMPTCQPGPNNNNNINNPNQNNMAAVGVAAGYMDLIGVPVSVDNPEYLLNAQTLGVGESPIPTQTIGIPVMGVPGTMEVKVPMPGSEPTSSDHEYYNDTQRELQPLHRNRNTETRV

Target ORF information:

RefSeq Version	NM_057411.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster epidermal growth factor receptor, transcript variant B (Egfr), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057411.4

ORF Insert Sequence:

ATGCTGCTGC GACGGCGCAA CGGCCCCTGC CCCTTCCCCC TGCTGCTCCT GCTCCTGGCC 
CACTGCATTT GCATTTGGCC CGCGTCGGCG GCCCGCGATC GCTACGCCCG CCAGAACAAT 
CGCCAGCGCC ATCAGGATAT AGATCGCGAT CGGGATCGAG ATCGATTCCT ATACCGCAGC 
AGTTCGGCCC AAAATCGACA GAGGGGCGGG GCCAACTTCG CCCTGGGACT GGGAGCCAAC 
GGAGTCACCA TTCCCACCAG TCTGGAGGAT AAGAACAAGA ACGAGTTCGT CAAGGGGAAA 
ATCTGCATCG GCACTAAATC TCGGCTCTCC GTGCCCTCCA ACAAGGAACA TCATTACCGG 
AACCTCAGAG ATCGGTACAC GAACTGTACG TATGTGGATG GCAACCTGGA GCTGACCTGG 
CTGCCCAACG AGAATTTGGA CCTCAGCTTC CTGGACAACA TACGGGAGGT CACCGGCTAT 
ATTCTGATCA GTCATGTGGA CGTTAAGAAA GTGGTATTTC CCAAACTACA AATCATTCGC 
GGACGCACGC TGTTCAGCTT ATCCGTGGAG GAGGAGAAGT ATGCCTTGTT CGTCACTTAT 
TCCAAAATGT ACACGCTGGA GATTCCCGAT CTACGCGATG TCTTAAATGG CCAAGTGGGC 
TTCCACAACA ACTACAATCT CTGCCACATG CGAACGATCC AGTGGTCGGA GATTGTATCC 
AACGGCACGG ATGCATACTA CAACTACGAC TTTACTGCTC CGGAGCGCGA GTGTCCCAAG 
TGCCACGAGA GCTGCACGCA CGGATGTTGG GGCGAGGGTC CCAAGAATTG CCAGAAGTTC 
AGCAAGCTCA CCTGCTCGCC ACAGTGTGCC GGAGGTCGTT GCTATGGACC AAAGCCGCGG 
GAGTGTTGTC ACCTCTTCTG CGCCGGAGGA TGCACTGGTC CCACGCAAAA GGATTGCATC 
GCCTGCAAGA ACTTCTTCGA CGAGGGCGTA TGCAAGGAGG AATGCCCGCC CATGCGCAAG 
TACAATCCCA CCACCTATGT TCTTGAAACG AATCCTGAGG GAAAGTATGC CTATGGTGCC 
ACCTGCGTCA AGGAGTGTCC CGGTCATCTG TTGCGTGATA ATGGCGCCTG CGTGCGCAGC 
TGTCCCCAGG ACAAGATGGA CAAGGGGGGC GAGTGTGTGC CCTGCAATGG ACCGTGCCCC 
AAAACCTGCC CGGGCGTTAC TGTCCTGCAT GCCGGCAACA TTGACTCGTT CCGGAATTGT 
ACGGTGATCG ATGGCAACAT TCGCATTTTG GATCAGACCT TCTCGGGCTT CCAGGATGTC 
TATGCCAACT ACACGATGGG ACCACGATAC ATACCGCTGG ATCCCGAGCG ACTGGAGGTG 
TTCTCCACGG TGAAGGAGAT CACCGGGTAT CTGAATATCG AGGGAACCCA CCCGCAGTTC 
CGGAATCTGT CGTACTTCCG CAATCTGGAA ACAATTCATG GCCGCCAGCT GATGGAGAGC 
ATGTTTGCCG CTTTGGCGAT CGTTAAGTCA TCCCTGTACA GCCTGGAGAT GCGCAATCTG 
AAGCAGATTA GTTCCGGCAG TGTGGTCATC CAGCATAATA GAGACCTCTG CTACGTAAGC 
AATATCCGTT GGCCGGCCAT TCAGAAGGAG CCCGAACAGA AGGTGTGGGT CAACGAGAAT 
CTCAGGGCGG ATCTATGCGA GAAAAATGGA ACCATTTGCT CGGATCAGTG CAACGAGGAC 
GGCTGCTGGG GAGCTGGCAC GGATCAGTGC CTTACCTGCA AGAACTTCAA TTTCAATGGC 
ACCTGCATCG CCGACTGTGG TTATATATCC AATGCCTACA AGTTTGACAA TAGAACGTGC 
AAGATATGCC ATCCAGAGTG CCGGACTTGC AATGGAGCTG GAGCAGATCA CTGCCAGGAG 
TGCGTCCATG TGAGGGACGG TCAGCACTGT GTGTCCGAGT GCCCGAAGAA CAAGTACAAC 
GATCGTGGTG TCTGCCGAGA GTGCCACGCC ACCTGCGATG GATGCACTGG GCCCAAGGAC 
ACCATCGGCA TTGGAGCGTG TACAACGTGC AATTTGGCCA TTATCAACAA TGACGCCACA 
GTAAAACGCT GCCTGCTGAA GGACGACAAG TGCCCCGATG GGTACTTCTG GGAGTATGTG 
CATCCACAAG AGCAGGGATC GCTAAAGCCA TTGGCCGGCA GAGCAGTTTG CCGAAAGTGC 
CATCCCCTTT GCGAGCTGTG CACCAACTAC GGATACCATG AACAGGTGTG CTCCAAGTGC 
ACCCACTACA AGCGACGAGA GCAGTGCGAG ACCGAGTGTC CGGCCGATCA CTACACGGAT 
GAGGAGCAGC GCGAGTGCTT CCAGTGCCAC CCAGAATGCA ACGGTTGCAC TGGTCCGGGT 
GCCGACGATT GCAAGTCTTG TCGCAACTTC AAGTTGTTCG ACGCGAATGA GACGGGTCCC 
TATGTGAACT CCACGATGTT CAATTGCACC TCGAAGTGTC CCTTGGAGAT GCGACATGTG 
AACTATCAGT ACACGGCCAT TGGACCCTAC TGTGCAGCTA GTCCGCCGAG GAGCAGCAAG 
ATAACTGCCA ATCTGGATGT GAACATGATC TTCATTATCA CTGGTGCTGT TCTGGTGCCG 
ACGATCTGCA TCCTCTGCGT GGTCACATAC ATTTGTCGGC AAAAGCAAAA GGCCAAGAAA 
GAAACAGTGA AGATGACCAT GGCTCTGTCC GGCTGTGAGG ATTCCGAGCC GCTGCGTCCC 
TCGAACATTG GAGCCAATCT ATGCAAGTTG CGCATTGTCA AGGACGCCGA GTTGCGCAAG 
GGCGGAGTCC TCGGAATGGG AGCCTTTGGA CGAGTGTACA AGGGCGTTTG GGTGCCGGAG 
GGTGAGAACG TCAAGATTCC AGTGGCCATT AAGGAGCTGC TCAAGTCCAC AGGCGCCGAG 
TCAAGCGAAG AGTTCCTCCG CGAAGCCTAC ATCATGGCCT CTGTGGAGCA CGTTAATCTG 
CTGAAGCTCC TGGCCGTCTG CATGTCCTCA CAAATGATGC TAATCACGCA ACTGATGCCG 
CTTGGCTGCC TGTTGGACTA TGTGCGAAAT AACCGGGACA AGATCGGCTC TAAGGCTCTG 
CTCAACTGGA GCACGCAAAT CGCCAAGGGC ATGTCGTATC TGGAGGAGAA GCGACTGGTC 
CACAGAGACT TGGCTGCCCG CAATGTCCTG GTGCAGACTC CCTCGCTGGT GAAGATCACC 
GACTTTGGGC TGGCCAAGTT GCTGAGCAGC GATTCCAATG AGTACAAGGC TGCTGGCGGC 
AAGATGCCCA TCAAGTGGTT GGCACTGGAG TGCATTCGCA ATCGTGTATT CACCAGCAAG 
TCCGATGTCT GGGCCTTTGG TGTGACAATT TGGGAACTGC TGACCTTTGG CCAGCGTCCA 
CACGAGAACA TCCCCGCTAA GGATATTCCC GATCTTATTG AAGTCGGTCT GAAGCTGGAG 
CAGCCGGAGA TTTGTTCGCT GGACATTTAC TGCACACTTC TCTCGTGCTG GCACTTGGAT 
GCCGCCATGC GTCCAACCTT CAAGCAGCTG ACTACGGTCT TTGCTGAGTT CGCCAGAGAT 
CCGGGTCGCT ATCTGGCCAT TCCCGGGGAT AAGTTCACCC GGCTGCCGGC CTACACGAGT 
CAGGATGAGA AGGATCTCAT CCGAAAATTG GCTCCCACCA CCGATGGGTC CGAAGCCATT 
GCGGAACCCG ATGACTACCT GCAACCCAAG GCAGCACCTG GTCCTAGTCA CAGAACCGAC 
TGCACGGATG AGATACCCAA GCTGAACCGC TACTGCAAGG ATCCTAGCAA CAAGAATTCG 
AGTACCGGAG ACGATGAGAC GGATTCGAGT GCCCGGGAAG TGGGCGTGGG TAATCTGCGC 
CTCGATCTAC CAGTCGATGA GGATGATTAC CTGATGCCCA CATGCCAACC GGGGCCCAAC 
AACAACAACA ACATAAATAA TCCCAATCAA AACAATATGG CAGCTGTGGG CGTGGCTGCC 
GGCTACATGG ATCTCATCGG AGTGCCCGTT AGTGTGGACA ATCCGGAGTA TCTGCTAAAC 
GCGCAGACAC TGGGTGTTGG GGAGTCGCCG ATACCCACCC AGACCATCGG GATACCGGTG 
ATGGGAGTCC CGGGCACCAT GGAGGTCAAG GTGCCAATGC CAGGCAGTGA GCCAACGAGC 
TCCGATCACG AGTACTACAA TGATACCCAA CGGGAGTTGC AGCCACTGCA TCGAAACCGC 
AACACGGAGA CGAGGGTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Egfr cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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