insc cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	insc
Entrez Gene ID	37355
Full Name	inscuteable
Synonyms	25/17,CG11312,Dmel\CG11312,INSC,Ins,Insc,fam,l(2)05475,l(2)k12405,nem
General protein information	Preferred Names inscuteable Names CG11312 gene product from transcript CG11312-RA CG11312-PA fata morgana insc-PA inscrutable inscutable not enough muscles
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 57B3-57B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_057676.4	NP_477024.1	inscuteable

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Inscuteable maintains type I neuroblast lineage identity via Numb/Notch signaling in the Drosophila larval brain
Title: Inscuteable maintains type I neuroblast lineage identity via Numb/Notch signaling in the Drosophila larval brain.

Insc-Pins complex requires Galphai for partial activity and that the complex specifically recruits Dlg but not Mud.
Title: Inscuteable regulates the Pins-Mud spindle orientation pathway.

Inscuteable and NuMA [Mud] proteins bind competitively to Leu-Gly-Asn repeat-enriched protein (LGN) during asymmetric cell divisions.
Title: Inscuteable and NuMA proteins bind competitively to Leu-Gly-Asn repeat-enriched protein (LGN) during asymmetric cell divisions.

The Drosophila Hem/Kette/Nap1 protein regulates asymmetric division of neural precursor cells by regulating localization of Inscuteable and Numb.
Title: The Drosophila Hem/Kette/Nap1 protein regulates asymmetric division of neural precursor cells by regulating localization of Inscuteable and Numb.

three neuroblast lineages (NB1-1, NB4-2, and NB7-1) require insc function for sibling cell fate specification in cells born from early ganglion mother cells (GMCs) whereas insc is not required in cells born from later GMCs of the same lineages
Title: On the roles of Notch, Delta, kuzbanian, and inscuteable during the development of Drosophila embryonic neuroblast lineages.

The following insc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the insc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa10708	NM_057676.4 Latest version!	Drosophila melanogaster inscuteable (insc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

insc ( NM_057676.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477024.1 Drosophila melanogaster inscuteable (insc), mRNA.

Price & Availability↑

CloneID	OFa10708
Clone ID Related Accession (Same CDS sequence)	NM_057676.4
Accession Version	NM_057676.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	inscuteable
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_057676.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCTTTC AGCGTAGCTA CAGCAAGGTC TGGTGGGGAT CCGAGCAGTC CTCCCTGACC 
AGCGGCGGTT TCTATTCGAG CTTCAGCGGC GGGAGCAGCA CATCCAGCAC ATCCAACTAC 
CCACAAAGAC AGCCAGCATC CCTCGGCCCC CTGGGCAATC TGTCCTGCAT CCAGGAGGTG 
GAGGACGAGC AGAACAGCTC CAAGCTATCC TGGCACAAGC ACGATAGCCC CGGCAGTCTG 
CGCAGTCAGG ATTCGGGCTT CTCGGACAAT GAGGAATCGC ATCAGTTGCA GCATCAGCAG 
AACTTATCCC CGCCATCGCC ACAATCCGAT AAGCAGCAAA CGCCCGCGGC CACGCCCACA 
TCCGTACATT CGGTGGCCAC GCCCCCAACT GTGGTGCGCC GAGTGGGCAA CTCGTCGTTC 
TACTCGCCGC TGATCAGTGT CACGCGCCGA ATTTCCTTCA CCAGCGGCCA GATTACGCCC 
AAGACGAGGT CGGGAGATGA GGATGCCGAG GATGCGGAAC TGGAGGCGAG TCGGAGTCGT 
CTGTTCGAGC AGCTGGATAG CATGAAGCTG GACGATGGAG AGGCAGAGGC CGAGGAGGAT 
TCACCACCGC GTCCGGCCAT AAGACGCCGC AGGCGTCTGA TGCGTAAACC CATGCCGGAA 
CCGGAAGCGG CTTCGGCCAG CGAGGAGGAG GAAGTGGTCA TAGCACCACC ACCACCGCCG 
CCGTACAACA ATGAAACTGT TTACTTGGGT GAGGCGCCAC CGCCGTACAA CAATGAAACG 
GTGACTTTTG GAGCCACCGA GCAGGATGAA AGCCTCCAGC AGCAACCTTC CCCCTTAAGA 
GTCAGATCCT TGCGTTTCAC AGCTAGCACC AGCACACCAA AAAGTGGGTC CAAGATTGCG 
AAACGGGGCA AGAAGCACCC AGAACCCGTG GCCAGTTGGA TGTCGGAACA GCGCTGGGCC 
GGCGAACCGG AGGTGATGTG CACCCTGCAG CACAAATCGA TTGCGCAGGA GGCATACAAG 
AACTACACCA TCACCACCAG TGCGGTTTGC AAGTTGGTCA GGCAACTGCA GCAGCAGGCT 
CTGTCGCTCC AAGTCCACTT CGAGCGGAGC GAGCGGGTTC TCAGCGGACT GCAGGCCAGT 
TCTCTGCCAG AAGCCCTTGC CGGAGCCACC CAGTTGCTGT CCCACCTGGA CGACTTCACC 
GCCACTCTGG AGCGACGAGG TGTGTTCTTC AACGATGCCA AGATCGAAAG GAGACGCTAT 
GAGCAGCACT TGGAGCAGAT CCGAACGGTG AGCAAGGATA CGAGGTACTC GCTGGAGCGA 
CAGCACTACA TCAATCTCGA ATCCCTGCTG GACGATGTAC AGCTCCTGAA GCGTCACACT 
CTGATCACCC TGCGTTTGAT CTTTGAGCGA CTGGTTCGCG TCCTGGTGAT CAGCATTGAG 
CAGAGTCAAT GTGATTTGCT ATTGCGGGCC AATATAAATA TGGTAGCCAC CTTGATGAAC 
ATTGACTACG ATGGATTTGC ATCGTTATCC GATGCCTTCG TCCAAAACGA GGCAGTGCGA 
ACCCTGCTCG TCGTTGTGCT GGATCACAAG CAGAGCTCCG TGAGGGCCTT GGCACTCCGG 
GCTCTGGCCA CCTTGTGCTG TGCTCCCCAA GCGATCAATC AACTGGGCAG TTGTGGAGGC 
ATCGAAATTG TAAGGGACAT CCTGCAGGTG GAATCCGCCG GCGAGAGAGG AGCCATCGAG 
CGAAGGGAGG CAGTTTCGCT GCTGGCCCAG ATTACCGCCG CTTGGCACGG ATCGGAGCAC 
AGGGTTCCGG GTCTGAGGGA TTGTGCTGAG AGTTTGGTAG CCGGTCTGGC CGCCCTCCTG 
CAGCCGGAAT GCTGCACCCA AACGCTGCTC CTCTGCGCCG CTGCCCTGAA CAACTTGAGC 
CGCATGGAGG CCACCTCCCA CTACTCCATC ATGTCCAACG AGGCCATCTT CCGGCTGATT 
GATACCCTGG AGCACCAGAC TTGCGGCACA AGCGTCTTTC TCTACGAGCA AATCGTTGGG 
ATGCTGCACA ACATGTCGCT GAACAAGAAG TGCCACAGCC ACTTGGCCAA CGGCGTCATC 
ATAAATTTCA TCACGTCCGT GTACCAAACG GAATTCTACC AAACCTACGG CTCACGTGCG 
GAAAGCGATG CCCAGCGCCG CACCATCAAG ACCATTCTGC ACACCCTGAC CCGGCTGGCC 
AGCGATTCGC CGACCCTCGG AGCAGAGCTC CTGGAGCAGT GGCATCTGCC GGACCTGCTG 
CGTCAATCCC TGGCCCTTAA GCCCGCCAAT TCGCAGCAGC AGCAGCACCT GGACAGCAGC 
TACGGCGGGG ACATATCCCA GTTAGCGCGA CAATTGCTCC GTGCCCATGG ACAGGAGCAG 
CAGCTGCTGC AGGCTACTCC GGCGCCAGGC GGATCCTTCT CCAGTGGCCA CCAGACGCCC 
GAGGCACAAA CCCAGACCAA TTCCAGTGGC ATGTCCAGCT CCGGCAGCAA AACCAAGGCC 
AAATCGTCGG CGTCGCCGCT GCTCAAATTC AATTTAACAC GTCAGGAGAG TTTCGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477024.1
CDS	362..2941
Translation	MSFQRSYSKVWWGSEQSSLTSGGFYSSFSGGSSTSSTSNYPQRQPASLGPLGNLSCIQEVEDEQNSSKLSWHKHDSPGSLRSQDSGFSDNEESHQLQHQQNLSPPSPQSDKQQTPAATPTSVHSVATPPTVVRRVGNSSFYSPLISVTRRISFTSGQITPKTRSGDEDAEDAELEASRSRLFEQLDSMKLDDGEAEAEEDSPPRPAIRRRRRLMRKPMPEPEAASASEEEEVVIAPPPPPPYNNETVYLGEAPPPYNNETVTFGATEQDESLQQQPSPLRVRSLRFTASTSTPKSGSKIAKRGKKHPEPVASWMSEQRWAGEPEVMCTLQHKSIAQEAYKNYTITTSAVCKLVRQLQQQALSLQVHFERSERVLSGLQASSLPEALAGATQLLSHLDDFTATLERRGVFFNDAKIERRRYEQHLEQIRTVSKDTRYSLERQHYINLESLLDDVQLLKRHTLITLRLIFERLVRVLVISIEQSQCDLLLRANINMVATLMNIDYDGFASLSDAFVQNEAVRTLLVVVLDHKQSSVRALALRALATLCCAPQAINQLGSCGGIEIVRDILQVESAGERGAIERREAVSLLAQITAAWHGSEHRVPGLRDCAESLVAGLAALLQPECCTQTLLLCAAALNNLSRMEATSHYSIMSNEAIFRLIDTLEHQTCGTSVFLYEQIVGMLHNMSLNKKCHSHLANGVIINFITSVYQTEFYQTYGSRAESDAQRRTIKTILHTLTRLASDSPTLGAELLEQWHLPDLLRQSLALKPANSQQQQHLDSSYGGDISQLARQLLRAHGQEQQLLQATPAPGGSFSSGHQTPEAQTQTNSSGMSSSGSKTKAKSSASPLLKFNLTRQESFV

Target ORF information:

RefSeq Version	NM_057676.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster inscuteable (insc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057676.4

ORF Insert Sequence:

ATGTCCTTTC AGCGTAGCTA CAGCAAGGTC TGGTGGGGAT CCGAGCAGTC CTCCCTGACC 
AGCGGCGGTT TCTATTCGAG CTTCAGCGGC GGGAGCAGCA CATCCAGCAC ATCCAACTAC 
CCACAAAGAC AGCCAGCATC CCTCGGCCCC CTGGGCAATC TGTCCTGCAT CCAGGAGGTG 
GAGGACGAGC AGAACAGCTC CAAGCTATCC TGGCACAAGC ACGATAGCCC CGGCAGTCTG 
CGCAGTCAGG ATTCGGGCTT CTCGGACAAT GAGGAATCGC ATCAGTTGCA GCATCAGCAG 
AACTTATCCC CGCCATCGCC ACAATCCGAT AAGCAGCAAA CGCCCGCGGC CACGCCCACA 
TCCGTACATT CGGTGGCCAC GCCCCCAACT GTGGTGCGCC GAGTGGGCAA CTCGTCGTTC 
TACTCGCCGC TGATCAGTGT CACGCGCCGA ATTTCCTTCA CCAGCGGCCA GATTACGCCC 
AAGACGAGGT CGGGAGATGA GGATGCCGAG GATGCGGAAC TGGAGGCGAG TCGGAGTCGT 
CTGTTCGAGC AGCTGGATAG CATGAAGCTG GACGATGGAG AGGCAGAGGC CGAGGAGGAT 
TCACCACCGC GTCCGGCCAT AAGACGCCGC AGGCGTCTGA TGCGTAAACC CATGCCGGAA 
CCGGAAGCGG CTTCGGCCAG CGAGGAGGAG GAAGTGGTCA TAGCACCACC ACCACCGCCG 
CCGTACAACA ATGAAACTGT TTACTTGGGT GAGGCGCCAC CGCCGTACAA CAATGAAACG 
GTGACTTTTG GAGCCACCGA GCAGGATGAA AGCCTCCAGC AGCAACCTTC CCCCTTAAGA 
GTCAGATCCT TGCGTTTCAC AGCTAGCACC AGCACACCAA AAAGTGGGTC CAAGATTGCG 
AAACGGGGCA AGAAGCACCC AGAACCCGTG GCCAGTTGGA TGTCGGAACA GCGCTGGGCC 
GGCGAACCGG AGGTGATGTG CACCCTGCAG CACAAATCGA TTGCGCAGGA GGCATACAAG 
AACTACACCA TCACCACCAG TGCGGTTTGC AAGTTGGTCA GGCAACTGCA GCAGCAGGCT 
CTGTCGCTCC AAGTCCACTT CGAGCGGAGC GAGCGGGTTC TCAGCGGACT GCAGGCCAGT 
TCTCTGCCAG AAGCCCTTGC CGGAGCCACC CAGTTGCTGT CCCACCTGGA CGACTTCACC 
GCCACTCTGG AGCGACGAGG TGTGTTCTTC AACGATGCCA AGATCGAAAG GAGACGCTAT 
GAGCAGCACT TGGAGCAGAT CCGAACGGTG AGCAAGGATA CGAGGTACTC GCTGGAGCGA 
CAGCACTACA TCAATCTCGA ATCCCTGCTG GACGATGTAC AGCTCCTGAA GCGTCACACT 
CTGATCACCC TGCGTTTGAT CTTTGAGCGA CTGGTTCGCG TCCTGGTGAT CAGCATTGAG 
CAGAGTCAAT GTGATTTGCT ATTGCGGGCC AATATAAATA TGGTAGCCAC CTTGATGAAC 
ATTGACTACG ATGGATTTGC ATCGTTATCC GATGCCTTCG TCCAAAACGA GGCAGTGCGA 
ACCCTGCTCG TCGTTGTGCT GGATCACAAG CAGAGCTCCG TGAGGGCCTT GGCACTCCGG 
GCTCTGGCCA CCTTGTGCTG TGCTCCCCAA GCGATCAATC AACTGGGCAG TTGTGGAGGC 
ATCGAAATTG TAAGGGACAT CCTGCAGGTG GAATCCGCCG GCGAGAGAGG AGCCATCGAG 
CGAAGGGAGG CAGTTTCGCT GCTGGCCCAG ATTACCGCCG CTTGGCACGG ATCGGAGCAC 
AGGGTTCCGG GTCTGAGGGA TTGTGCTGAG AGTTTGGTAG CCGGTCTGGC CGCCCTCCTG 
CAGCCGGAAT GCTGCACCCA AACGCTGCTC CTCTGCGCCG CTGCCCTGAA CAACTTGAGC 
CGCATGGAGG CCACCTCCCA CTACTCCATC ATGTCCAACG AGGCCATCTT CCGGCTGATT 
GATACCCTGG AGCACCAGAC TTGCGGCACA AGCGTCTTTC TCTACGAGCA AATCGTTGGG 
ATGCTGCACA ACATGTCGCT GAACAAGAAG TGCCACAGCC ACTTGGCCAA CGGCGTCATC 
ATAAATTTCA TCACGTCCGT GTACCAAACG GAATTCTACC AAACCTACGG CTCACGTGCG 
GAAAGCGATG CCCAGCGCCG CACCATCAAG ACCATTCTGC ACACCCTGAC CCGGCTGGCC 
AGCGATTCGC CGACCCTCGG AGCAGAGCTC CTGGAGCAGT GGCATCTGCC GGACCTGCTG 
CGTCAATCCC TGGCCCTTAA GCCCGCCAAT TCGCAGCAGC AGCAGCACCT GGACAGCAGC 
TACGGCGGGG ACATATCCCA GTTAGCGCGA CAATTGCTCC GTGCCCATGG ACAGGAGCAG 
CAGCTGCTGC AGGCTACTCC GGCGCCAGGC GGATCCTTCT CCAGTGGCCA CCAGACGCCC 
GAGGCACAAA CCCAGACCAA TTCCAGTGGC ATGTCCAGCT CCGGCAGCAA AACCAAGGCC 
AAATCGTCGG CGTCGCCGCT GCTCAAATTC AATTTAACAC GTCAGGAGAG TTTCGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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insc cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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