side-VIII cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	side-VIII
Entrez Gene ID	37310
Full Name	CG12484 gene product from transcript CG12484-RB
Synonyms	12484,CG30143,CG33041,CT32777,Dmel\CG12484,Dmel_CG30143,Dmel_CG33041
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 57A3-57A3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001144248.2	NP_001137720.1	sidestep VIII, isoform D
NM_001103912.3	NP_001097382.2	sidestep VIII, isoform B
NM_001103913.3	NP_001097383.3	sidestep VIII, isoform E
NM_166392.4	NP_725963.3	sidestep VIII, isoform A

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG12484	NP_001097382.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002104	XP_320937.5

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following side-VIII gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the side-VIII cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa13143	NM_166392.4 Latest version!	Drosophila melanogaster sidestep VIII (side-VIII), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa13140	NM_001103912.3 Latest version!	Drosophila melanogaster sidestep VIII (side-VIII), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OFa13141	NM_001144248.2 Latest version!	Drosophila melanogaster sidestep VIII (side-VIII), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OFa21876	NM_001103913.3 Latest version!	Drosophila melanogaster sidestep VIII (side-VIII), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

side-VIII ( NM_166392.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725963.3 Drosophila melanogaster sidestep VIII (side-VIII), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa13143
Clone ID Related Accession (Same CDS sequence)	NM_166392.4
Accession Version	NM_166392.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	sidestep VIII, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166392.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCAGGA CCTCCAGCCG CTCGCTCTTC CTGCCGCTCT GCCTGCTCGT CCTGTCCGCG 
CTAAATGGCC CAGCGGCGGC GGCATCTCGC GTCCGCGTCA GCTCCTCGAC CACCATGACC 
AGGAACATCG AGGAGGAGAA TGCCCTACTT TTACTTTTAG CTGGACCTCC CGTGCTATCT 
GAGTCCATAA TGGGTACGGT CGGCCGGCTG CCCTGCAACG TCACCCCGCC CATTTACGAG 
GATCGAGTGG CGCTGGTTAT CTGGTACAAA GTTGGCCTGA AGACTCCCAT TTACAGTGTG 
GACACACGCG ACTCGAACTT CGCCCAGGGA ACGCACTGGT CGGATGAGAC CTACCGGGAG 
CGGCTCTCCT TCCACGTGGA AGGACGTGCT GGCACGCTGA CCATCAAGTC AACCACGGAG 
GACGACACGG GCGAGTATCG CTGCCGCGTC GACTTCCAGA AGAGTCCGAC TCGCAACTCC 
AAAGTGAATT TAACTGTCAT AATTCCGCCC GAGTCGGTGA TTATATTGGA CAGCAAGGGT 
GTGACCATCG AGGATCACAC GCTGGGTCCT TACAACGAGG GCTCTGGGAT AAACATCACG 
TGCGTGGCCA TCGGCGGCCG CCCGCAGCCG AGGGTTACCT GGCTGCATGG AAACACCGTC 
TACAAGAATG CCAGTGTGGG CCAACCCCTG TCCGAGCGAC GGGTGGGCAA TACCCTGTCC 
TTGGCTCGAC TGGAGCGCAG GAATCTTCAC ATGCAGCTAA CGTGCCGGGC GGAGAACAAT 
AATTTGACAA CGCCAATTAT CAGCAGCGTC GTCCTCGACA TGAACCTGCG TCCTTTGATT 
GTGAAGCTGC AGGGGGAGAA TCGAGCTCTG TCGGCGGGGA ATTCTTATCA GCTCAGTTGC 
GTTGTCATCG GAGCACGTCC AGCGCCGACG ATTACCTGGT GGAAGGGCAG TACCCCCATG 
AAGAATACTC ACGAGATTGC TACACCGGAC GGTAATCTGA CTACCTCGGT GCTGACATTC 
ACGCCCACCA TCGATGACCG CGGCAAGTTC CTCTCGTGTC GCGCGGAGCA GAGCATGATA 
CCCGAGTCCG GAATGGAGGA CGGCTGGAAA TTGGACATAT ATCACATACC GGTGGTGAGC 
CTGGAGCTGG GAACCAATTC GCTTAACTCA ACTCTGCGCG AGGGAATCGA TGTCTTCTTC 
GAGTGCAACA TCAAATCGAA TCCCTGGATA TACGAAGTTA GCTGGCGGCA CAATGGTAAG 
ATTCTAACCA ACAATCCAGC CGAAGGCATC GCCGTCTCCA ACCAGAGTCT GGTGCTTCAA 
AATGCCAGCC GGGCGAGAAG TGGCATCTAC ACCTGTGTGG GCAGTAATCG GGAGGGCGAC 
GGCGAGAGCA ATCCCGTTCA GCTGGACATA CGATTTGCTC CTGTTTGCCG ACCTCGCCAG 
CGTCTCTCCT ATAGCTCGGG CAGGCATGAG ACTGTCAAGG TGGCCTGCGA AATTGATGCC 
AATCCCGCGG AGGCCACCTA CGTTTGGAAA TTCAATGCAA CCCAGGGCGA AACAGTTGAC 
ATACCGGCTT CGCAAGTGGC CGTGGATCGG GGCCGAAGTA TAGCCCATTA CACGCCCATG 
ACGGAGAATG ACTACGGTAC GCTGCTCTGC TGGGCCACCA ACGAAATTGG CGATCAAAGT 
GAACCCTGCG TATACACAAT ATTCCCAGCA GGCGAGCCGG ATCCGTTGCT AAACTGCACG 
GTGCTCAACC AGACATCGAC GGGCTTCCAA ATCGAATGCA TCGAGGGCTT CAATGGCGGC 
CTGCAGCAGG ACTTTATAAT GGAGGTTTAC ATGAACGGAA CGACACGACA TCCCAAAATT 
TCAAAATCAA AGCGACCGTA CTTTGAGGTA AGCGGACTGG TGCCCGGAAT GGGCTACAAT 
GTCTTCCTCA TTGCCAACAA CAGCAAGGGC CGTAGCAATG CCACCATTTT GCAGGTCTAC 
ACGCTGAAGG ATCCGGAGAA GCAGACGGTT AAAATAACAT TCACTAAGTC CTTCCAAGGA 
CTTAGGCCTG CCTATACCAA AACAAAAGCG ACAGCGACCA CGACCAGCGA CTGCGTTTGC 
GATGAGGATG AGTTCCTAAA TCTGGGCAAG GGAATTAGCC ACGACGAGGG CACAGACGCT 
GACCAGCAAG AAGGTAGCGG TGGACTATCC TTTTCTATAA TTTTCTATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725963.3
CDS	551..2761
Translation	MSRTSSRSLFLPLCLLVLSALNGPAAAASRVRVSSSTTMTRNIEEENALLLLLAGPPVLSESIMGTVGRLPCNVTPPIYEDRVALVIWYKVGLKTPIYSVDTRDSNFAQGTHWSDETYRERLSFHVEGRAGTLTIKSTTEDDTGEYRCRVDFQKSPTRNSKVNLTVIIPPESVIILDSKGVTIEDHTLGPYNEGSGINITCVAIGGRPQPRVTWLHGNTVYKNASVGQPLSERRVGNTLSLARLERRNLHMQLTCRAENNNLTTPIISSVVLDMNLRPLIVKLQGENRALSAGNSYQLSCVVIGARPAPTITWWKGSTPMKNTHEIATPDGNLTTSVLTFTPTIDDRGKFLSCRAEQSMIPESGMEDGWKLDIYHIPVVSLELGTNSLNSTLREGIDVFFECNIKSNPWIYEVSWRHNGKILTNNPAEGIAVSNQSLVLQNASRARSGIYTCVGSNREGDGESNPVQLDIRFAPVCRPRQRLSYSSGRHETVKVACEIDANPAEATYVWKFNATQGETVDIPASQVAVDRGRSIAHYTPMTENDYGTLLCWATNEIGDQSEPCVYTIFPAGEPDPLLNCTVLNQTSTGFQIECIEGFNGGLQQDFIMEVYMNGTTRHPKISKSKRPYFEVSGLVPGMGYNVFLIANNSKGRSNATILQVYTLKDPEKQTVKITFTKSFQGLRPAYTKTKATATTTSDCVCDEDEFLNLGKGISHDEGTDADQQEGSGGLSFSIIFY

Target ORF information:

RefSeq Version	NM_166392.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster sidestep VIII (side-VIII), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166392.4

ORF Insert Sequence:

ATGAGCAGGA CCTCCAGCCG CTCGCTCTTC CTGCCGCTCT GCCTGCTCGT CCTGTCCGCG 
CTAAATGGCC CAGCGGCGGC GGCATCTCGC GTCCGCGTCA GCTCCTCGAC CACCATGACC 
AGGAACATCG AGGAGGAGAA TGCCCTACTT TTACTTTTAG CTGGACCTCC CGTGCTATCT 
GAGTCCATAA TGGGTACGGT CGGCCGGCTG CCCTGCAACG TCACCCCGCC CATTTACGAG 
GATCGAGTGG CGCTGGTTAT CTGGTACAAA GTTGGCCTGA AGACTCCCAT TTACAGTGTG 
GACACACGCG ACTCGAACTT CGCCCAGGGA ACGCACTGGT CGGATGAGAC CTACCGGGAG 
CGGCTCTCCT TCCACGTGGA AGGACGTGCT GGCACGCTGA CCATCAAGTC AACCACGGAG 
GACGACACGG GCGAGTATCG CTGCCGCGTC GACTTCCAGA AGAGTCCGAC TCGCAACTCC 
AAAGTGAATT TAACTGTCAT AATTCCGCCC GAGTCGGTGA TTATATTGGA CAGCAAGGGT 
GTGACCATCG AGGATCACAC GCTGGGTCCT TACAACGAGG GCTCTGGGAT AAACATCACG 
TGCGTGGCCA TCGGCGGCCG CCCGCAGCCG AGGGTTACCT GGCTGCATGG AAACACCGTC 
TACAAGAATG CCAGTGTGGG CCAACCCCTG TCCGAGCGAC GGGTGGGCAA TACCCTGTCC 
TTGGCTCGAC TGGAGCGCAG GAATCTTCAC ATGCAGCTAA CGTGCCGGGC GGAGAACAAT 
AATTTGACAA CGCCAATTAT CAGCAGCGTC GTCCTCGACA TGAACCTGCG TCCTTTGATT 
GTGAAGCTGC AGGGGGAGAA TCGAGCTCTG TCGGCGGGGA ATTCTTATCA GCTCAGTTGC 
GTTGTCATCG GAGCACGTCC AGCGCCGACG ATTACCTGGT GGAAGGGCAG TACCCCCATG 
AAGAATACTC ACGAGATTGC TACACCGGAC GGTAATCTGA CTACCTCGGT GCTGACATTC 
ACGCCCACCA TCGATGACCG CGGCAAGTTC CTCTCGTGTC GCGCGGAGCA GAGCATGATA 
CCCGAGTCCG GAATGGAGGA CGGCTGGAAA TTGGACATAT ATCACATACC GGTGGTGAGC 
CTGGAGCTGG GAACCAATTC GCTTAACTCA ACTCTGCGCG AGGGAATCGA TGTCTTCTTC 
GAGTGCAACA TCAAATCGAA TCCCTGGATA TACGAAGTTA GCTGGCGGCA CAATGGTAAG 
ATTCTAACCA ACAATCCAGC CGAAGGCATC GCCGTCTCCA ACCAGAGTCT GGTGCTTCAA 
AATGCCAGCC GGGCGAGAAG TGGCATCTAC ACCTGTGTGG GCAGTAATCG GGAGGGCGAC 
GGCGAGAGCA ATCCCGTTCA GCTGGACATA CGATTTGCTC CTGTTTGCCG ACCTCGCCAG 
CGTCTCTCCT ATAGCTCGGG CAGGCATGAG ACTGTCAAGG TGGCCTGCGA AATTGATGCC 
AATCCCGCGG AGGCCACCTA CGTTTGGAAA TTCAATGCAA CCCAGGGCGA AACAGTTGAC 
ATACCGGCTT CGCAAGTGGC CGTGGATCGG GGCCGAAGTA TAGCCCATTA CACGCCCATG 
ACGGAGAATG ACTACGGTAC GCTGCTCTGC TGGGCCACCA ACGAAATTGG CGATCAAAGT 
GAACCCTGCG TATACACAAT ATTCCCAGCA GGCGAGCCGG ATCCGTTGCT AAACTGCACG 
GTGCTCAACC AGACATCGAC GGGCTTCCAA ATCGAATGCA TCGAGGGCTT CAATGGCGGC 
CTGCAGCAGG ACTTTATAAT GGAGGTTTAC ATGAACGGAA CGACACGACA TCCCAAAATT 
TCAAAATCAA AGCGACCGTA CTTTGAGGTA AGCGGACTGG TGCCCGGAAT GGGCTACAAT 
GTCTTCCTCA TTGCCAACAA CAGCAAGGGC CGTAGCAATG CCACCATTTT GCAGGTCTAC 
ACGCTGAAGG ATCCGGAGAA GCAGACGGTT AAAATAACAT TCACTAAGTC CTTCCAAGGA 
CTTAGGCCTG CCTATACCAA AACAAAAGCG ACAGCGACCA CGACCAGCGA CTGCGTTTGC 
GATGAGGATG AGTTCCTAAA TCTGGGCAAG GGAATTAGCC ACGACGAGGG CACAGACGCT 
GACCAGCAAG AAGGTAGCGG TGGACTATCC TTTTCTATAA TTTTCTATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

side-VIII ( NM_001103912.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097382.2 Drosophila melanogaster sidestep VIII (side-VIII), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa13140
Clone ID Related Accession (Same CDS sequence)	NM_001103912.3
Accession Version	NM_001103912.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	sidestep VIII, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001103912.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCAGGA CCTCCAGCCG CTCGCTCTTC CTGCCGCTCT GCCTGCTCGT CCTGTCCGCG 
CTAAATGGCC CAGCGGCGGC GGCATCTCGC GTCCGCGTCA GCTCCTCGAC CACCATGACC 
AGGAACATCG AGGAGGAGAA TGCCCTACTT TTACTTTTAG CTGGACCTCC CGTGCTATCT 
GAGTCCATAA TGGGTACGGT CGGCCGGCTG CCCTGCAACG TCACCCCGCC CATTTACGAG 
GATCGAGTGG CGCTGGTTAT CTGGTACAAA GTTGGCCTGA AGACTCCCAT TTACAGTGTG 
GACACACGCG ACTCGAACTT CGCCCAGGGA ACGCACTGGT CGGATGAGAC CTACCGGGAG 
CGGCTCTCCT TCCACGTGGA AGGACGTGCT GGCACGCTGA CCATCAAGTC AACCACGGAG 
GACGACACGG GCGAGTATCG CTGCCGCGTC GACTTCCAGA AGAGTCCGAC TCGCAACTCC 
AAAGTGAATT TAACTGTCAT AATTCCGCCC GAGTCGGTGA TTATATTGGA CAGCAAGGGT 
GTGACCATCG AGGATCACAC GCTGGGTCCT TACAACGAGG GCTCTGGGAT AAACATCACG 
TGCGTGGCCA TCGGCGGCCG CCCGCAGCCG AGGGTTACCT GGCTGCATGG AAACACCGTC 
TACAAGAATG CCAGTGTGGG CCAACCCCTG TCCGAGCGAC GGGTGGGCAA TACCCTGTCC 
TTGGCTCGAC TGGAGCGCAG GAATCTTCAC ATGCAGCTAA CGTGCCGGGC GGAGAACAAT 
AATTTGACAA CGCCAATTAT CAGCAGCGTC GTCCTCGACA TGAACCTGCG TCCTTTGATT 
GTGAAGCTGC AGGGGGAGAA TCGAGCTCTG TCGGCGGGGA ATTCTTATCA GCTCAGTTGC 
GTTGTCATCG GAGCACGTCC AGCGCCGACG ATTACCTGGT GGAAGGGCAG TACCCCCATG 
AAGAATACTC ACGAGATTGC TACACCGGAC GGTAATCTGA CTACCTCGGT GCTGACATTC 
ACGCCCACCA TCGATGACCG CGGCAAGTTC CTCTCGTGTC GCGCGGAGCA GAGCATGATA 
CCCGAGTCCG GAATGGAGGA CGGCTGGAAA TTGGACATAT ATCACATACC GGTGGTGAGC 
CTGGAGCTGG GAACCAATTC GCTTAACTCA ACTCTGCGCG AGGGAATCGA TGTCTTCTTC 
GAGTGCAACA TCAAATCGAA TCCCTGGATA TACGAAGTTA GCTGGCGGCA CAATGGTAAG 
ATTCTAACCA ACAATCCAGC CGAAGGCATC GCCGTCTCCA ACCAGAGTCT GGTGCTTCAA 
AATGCCAGCC GGGCGAGAAG TGGCATCTAC ACCTGTGTGG GCAGTAATCG GGAGGGCGAC 
GGCGAGAGCA ATCCCGTTCA GCTGGACATA CGATTTGCTC CTGTTTGCCG ACCTCGCCAG 
CGTCTCTCCT ATAGCTCGGG CAGGCATGAG ACTGTCAAGG TGGCCTGCGA AATTGATGCC 
AATCCCGCGG AGGCCACCTA CGTTTGGAAA TTCAATGCAA CCCAGGGCGA AACAGTTGAC 
ATACCGGCTT CGCAAGTGGC CGTGGATCGG GGCCGAAGTA TAGCCCATTA CACGCCCATG 
ACGGAGAATG ACTACGGTAC GCTGCTCTGC TGGGCCACCA ACGAAATTGG CGATCAAAGT 
GAACCCTGCG TATACACAAT ATTCCCAGCA GGCGAGCCGG ATCCGTTGCT AAACTGCACG 
GTGCTCAACC AGACATCGAC GGGCTTCCAA ATCGAATGCA TCGAGGGCTT CAATGGCGGC 
CTGCAGCAGG ACTTTATAAT GGAGGTTTAC ATGAACGGAA CGACACGACA TCCCAAAATT 
TCAAAATCAA AGCGACCGTA CTTTGAGGTA AGCGGACTGG TGCCCGGAAT GGGCTACAAT 
GTCTTCCTCA TTGCCAACAA CAGCAAGGGC CGTAGCAATG CCACCATTTT GCAGGTCTAC 
ACGCTGAAGG ATCCGGAGAA GCAGACGGTT AAAATAACAT TCACTAAGTC CTTCCAAGGA 
CTTAGGCCTG CCTATACCAA AACAAAAGCG ACAGCGACCA CGACCAGCGA CTGCGTTTGC 
GATGAGGATG AGTTCCTAAA TCTGGGCAAG GGAATTAGCC ACGACGAGGG CACAGACGCT 
GACCAGCAAG AAGACCTCTC GTTGGCCTAT GCCCCCGTCA TCGAGGACAT CCGGCCATTC 
CTCGGCATTC TGGCCGGCAT CGTGGGCAGC ATTTTCCTGG TGGCCCTCAT CATTGTGATT 
GTGGTGCGTG TGCGCGGCTC TTCGGGGCGC GATCGCAATA ATTACTCACA TCCGGGAAGC 
GGCGTCGGCG GCGTGGGCGG CGTGGGCGGC ACCACCGGCA ACGGCAGTGG GATGGGCGGC 
GGTGGCGGCG GTGTGGGCGG CACCACGGCC AACGGGAATG GCAGTCTGGG GCTGCTGGCT 
AGCAACAACA ATGGCAGCCT CGTCATCGGC ACCCTGGGCC ACAATGGTGG GCAATCAGTG 
CAGGATATGC ACCGCATCGG CCGGGAGACG TGCCATGTGA CGAGCAGTCT GGACAGCATC 
GACAAGAATC CGGACATCAT ACCGCAAGAC GGACACGACC TAGACGATGA GTGGACGACA 
AAGGGACATG GTCGCACTTA CGCCACGGCA GCGATGGCCG AGCAAAATGC CGTGATAACC 
TCCGGCACCT ATGATCACCT GATGCCCACG TACGCCGTTG TCGACAAGAA GACAGCACCG 
CCTCCCGGCC ATGGCCAGTA CATCCAGTAT AATACACTCA TTCCCGTTAG CAAAATGGGC 
GCTTATGCCA ATCAACAGCA GCAGCTCCAG CAGCAGCCGC AACAGAAGAC TGAGCTCAGT 
TATAGCGAAC TAAGCGCTCC TCTCGTCAGC GGCCCGGTGG GCGTTCAACG GATGGCACCC 
TACTGCAGCG CGACCTTGGG ACGGCCGGGC AGGCAGGCGG AACTTAAGCG TGCGGAGCCG 
AACATCTACT CGCAGGTAAA TGTTATGATG GACGACGAGA TTCTGGCCGA CTTGCAGGCG 
GCCACAGCAT CCGGCAGAAG CTATGTGGCT GGAGCCGTGG TCGACAATGT GGGCGGAGCG 
CCCAATCTCT ACGTTCAAGG CTATGCGACG CGTATCGATC TGGCACATCA TCCCATGCCC 
ATGTACTCAA CGAGCCCGAT GTTTGGCACG AACAGCACCA TCACCGGAGT CACCATGTCG 
CATCCATCAC AATTGGCCAC CTTCTCGCCG ACGCCGCCGC CGCCCATCTT CACCCACAGT 
ATGGTGACCA CTGGCGATGG GCTCCTGCAG CCGGTGACGT GCATGGGCCT GGTGGCAACA 
TCTTCGGCAG GGGCATCGGT TCCTGGTCCT GTTCCACTGG CCCCGCAGCA ACAGCAACAG 
CAGCAGCTGA CAGCGGCCAA TGGCGGAAAC GGAAGCATCG TCGGCATGGG CATGAGCCTT 
TATGGCAGCG ATGTGGGCAA ACCACAGTTG CTAAAGACCG TACCGGAGGA GGTTCACCAT 
CAGCTGAACG GGGAGGATAT CCTGATCGCC CAGGATCGCA ATCTCGGATC GGGAACTCGC 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097382.2
CDS	551..4156
Translation	MSRTSSRSLFLPLCLLVLSALNGPAAAASRVRVSSSTTMTRNIEEENALLLLLAGPPVLSESIMGTVGRLPCNVTPPIYEDRVALVIWYKVGLKTPIYSVDTRDSNFAQGTHWSDETYRERLSFHVEGRAGTLTIKSTTEDDTGEYRCRVDFQKSPTRNSKVNLTVIIPPESVIILDSKGVTIEDHTLGPYNEGSGINITCVAIGGRPQPRVTWLHGNTVYKNASVGQPLSERRVGNTLSLARLERRNLHMQLTCRAENNNLTTPIISSVVLDMNLRPLIVKLQGENRALSAGNSYQLSCVVIGARPAPTITWWKGSTPMKNTHEIATPDGNLTTSVLTFTPTIDDRGKFLSCRAEQSMIPESGMEDGWKLDIYHIPVVSLELGTNSLNSTLREGIDVFFECNIKSNPWIYEVSWRHNGKILTNNPAEGIAVSNQSLVLQNASRARSGIYTCVGSNREGDGESNPVQLDIRFAPVCRPRQRLSYSSGRHETVKVACEIDANPAEATYVWKFNATQGETVDIPASQVAVDRGRSIAHYTPMTENDYGTLLCWATNEIGDQSEPCVYTIFPAGEPDPLLNCTVLNQTSTGFQIECIEGFNGGLQQDFIMEVYMNGTTRHPKISKSKRPYFEVSGLVPGMGYNVFLIANNSKGRSNATILQVYTLKDPEKQTVKITFTKSFQGLRPAYTKTKATATTTSDCVCDEDEFLNLGKGISHDEGTDADQQEDLSLAYAPVIEDIRPFLGILAGIVGSIFLVALIIVIVVRVRGSSGRDRNNYSHPGSGVGGVGGVGGTTGNGSGMGGGGGGVGGTTANGNGSLGLLASNNNGSLVIGTLGHNGGQSVQDMHRIGRETCHVTSSLDSIDKNPDIIPQDGHDLDDEWTTKGHGRTYATAAMAEQNAVITSGTYDHLMPTYAVVDKKTAPPPGHGQYIQYNTLIPVSKMGAYANQQQQLQQQPQQKTELSYSELSAPLVSGPVGVQRMAPYCSATLGRPGRQAELKRAEPNIYSQVNVMMDDEILADLQAATASGRSYVAGAVVDNVGGAPNLYVQGYATRIDLAHHPMPMYSTSPMFGTNSTITGVTMSHPSQLATFSPTPPPPIFTHSMVTTGDGLLQPVTCMGLVATSSAGASVPGPVPLAPQQQQQQQLTAANGGNGSIVGMGMSLYGSDVGKPQLLKTVPEEVHHQLNGEDILIAQDRNLGSGTRF

Target ORF information:

RefSeq Version	NM_001103912.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster sidestep VIII (side-VIII), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103912.3

ORF Insert Sequence:

ATGAGCAGGA CCTCCAGCCG CTCGCTCTTC CTGCCGCTCT GCCTGCTCGT CCTGTCCGCG 
CTAAATGGCC CAGCGGCGGC GGCATCTCGC GTCCGCGTCA GCTCCTCGAC CACCATGACC 
AGGAACATCG AGGAGGAGAA TGCCCTACTT TTACTTTTAG CTGGACCTCC CGTGCTATCT 
GAGTCCATAA TGGGTACGGT CGGCCGGCTG CCCTGCAACG TCACCCCGCC CATTTACGAG 
GATCGAGTGG CGCTGGTTAT CTGGTACAAA GTTGGCCTGA AGACTCCCAT TTACAGTGTG 
GACACACGCG ACTCGAACTT CGCCCAGGGA ACGCACTGGT CGGATGAGAC CTACCGGGAG 
CGGCTCTCCT TCCACGTGGA AGGACGTGCT GGCACGCTGA CCATCAAGTC AACCACGGAG 
GACGACACGG GCGAGTATCG CTGCCGCGTC GACTTCCAGA AGAGTCCGAC TCGCAACTCC 
AAAGTGAATT TAACTGTCAT AATTCCGCCC GAGTCGGTGA TTATATTGGA CAGCAAGGGT 
GTGACCATCG AGGATCACAC GCTGGGTCCT TACAACGAGG GCTCTGGGAT AAACATCACG 
TGCGTGGCCA TCGGCGGCCG CCCGCAGCCG AGGGTTACCT GGCTGCATGG AAACACCGTC 
TACAAGAATG CCAGTGTGGG CCAACCCCTG TCCGAGCGAC GGGTGGGCAA TACCCTGTCC 
TTGGCTCGAC TGGAGCGCAG GAATCTTCAC ATGCAGCTAA CGTGCCGGGC GGAGAACAAT 
AATTTGACAA CGCCAATTAT CAGCAGCGTC GTCCTCGACA TGAACCTGCG TCCTTTGATT 
GTGAAGCTGC AGGGGGAGAA TCGAGCTCTG TCGGCGGGGA ATTCTTATCA GCTCAGTTGC 
GTTGTCATCG GAGCACGTCC AGCGCCGACG ATTACCTGGT GGAAGGGCAG TACCCCCATG 
AAGAATACTC ACGAGATTGC TACACCGGAC GGTAATCTGA CTACCTCGGT GCTGACATTC 
ACGCCCACCA TCGATGACCG CGGCAAGTTC CTCTCGTGTC GCGCGGAGCA GAGCATGATA 
CCCGAGTCCG GAATGGAGGA CGGCTGGAAA TTGGACATAT ATCACATACC GGTGGTGAGC 
CTGGAGCTGG GAACCAATTC GCTTAACTCA ACTCTGCGCG AGGGAATCGA TGTCTTCTTC 
GAGTGCAACA TCAAATCGAA TCCCTGGATA TACGAAGTTA GCTGGCGGCA CAATGGTAAG 
ATTCTAACCA ACAATCCAGC CGAAGGCATC GCCGTCTCCA ACCAGAGTCT GGTGCTTCAA 
AATGCCAGCC GGGCGAGAAG TGGCATCTAC ACCTGTGTGG GCAGTAATCG GGAGGGCGAC 
GGCGAGAGCA ATCCCGTTCA GCTGGACATA CGATTTGCTC CTGTTTGCCG ACCTCGCCAG 
CGTCTCTCCT ATAGCTCGGG CAGGCATGAG ACTGTCAAGG TGGCCTGCGA AATTGATGCC 
AATCCCGCGG AGGCCACCTA CGTTTGGAAA TTCAATGCAA CCCAGGGCGA AACAGTTGAC 
ATACCGGCTT CGCAAGTGGC CGTGGATCGG GGCCGAAGTA TAGCCCATTA CACGCCCATG 
ACGGAGAATG ACTACGGTAC GCTGCTCTGC TGGGCCACCA ACGAAATTGG CGATCAAAGT 
GAACCCTGCG TATACACAAT ATTCCCAGCA GGCGAGCCGG ATCCGTTGCT AAACTGCACG 
GTGCTCAACC AGACATCGAC GGGCTTCCAA ATCGAATGCA TCGAGGGCTT CAATGGCGGC 
CTGCAGCAGG ACTTTATAAT GGAGGTTTAC ATGAACGGAA CGACACGACA TCCCAAAATT 
TCAAAATCAA AGCGACCGTA CTTTGAGGTA AGCGGACTGG TGCCCGGAAT GGGCTACAAT 
GTCTTCCTCA TTGCCAACAA CAGCAAGGGC CGTAGCAATG CCACCATTTT GCAGGTCTAC 
ACGCTGAAGG ATCCGGAGAA GCAGACGGTT AAAATAACAT TCACTAAGTC CTTCCAAGGA 
CTTAGGCCTG CCTATACCAA AACAAAAGCG ACAGCGACCA CGACCAGCGA CTGCGTTTGC 
GATGAGGATG AGTTCCTAAA TCTGGGCAAG GGAATTAGCC ACGACGAGGG CACAGACGCT 
GACCAGCAAG AAGACCTCTC GTTGGCCTAT GCCCCCGTCA TCGAGGACAT CCGGCCATTC 
CTCGGCATTC TGGCCGGCAT CGTGGGCAGC ATTTTCCTGG TGGCCCTCAT CATTGTGATT 
GTGGTGCGTG TGCGCGGCTC TTCGGGGCGC GATCGCAATA ATTACTCACA TCCGGGAAGC 
GGCGTCGGCG GCGTGGGCGG CGTGGGCGGC ACCACCGGCA ACGGCAGTGG GATGGGCGGC 
GGTGGCGGCG GTGTGGGCGG CACCACGGCC AACGGGAATG GCAGTCTGGG GCTGCTGGCT 
AGCAACAACA ATGGCAGCCT CGTCATCGGC ACCCTGGGCC ACAATGGTGG GCAATCAGTG 
CAGGATATGC ACCGCATCGG CCGGGAGACG TGCCATGTGA CGAGCAGTCT GGACAGCATC 
GACAAGAATC CGGACATCAT ACCGCAAGAC GGACACGACC TAGACGATGA GTGGACGACA 
AAGGGACATG GTCGCACTTA CGCCACGGCA GCGATGGCCG AGCAAAATGC CGTGATAACC 
TCCGGCACCT ATGATCACCT GATGCCCACG TACGCCGTTG TCGACAAGAA GACAGCACCG 
CCTCCCGGCC ATGGCCAGTA CATCCAGTAT AATACACTCA TTCCCGTTAG CAAAATGGGC 
GCTTATGCCA ATCAACAGCA GCAGCTCCAG CAGCAGCCGC AACAGAAGAC TGAGCTCAGT 
TATAGCGAAC TAAGCGCTCC TCTCGTCAGC GGCCCGGTGG GCGTTCAACG GATGGCACCC 
TACTGCAGCG CGACCTTGGG ACGGCCGGGC AGGCAGGCGG AACTTAAGCG TGCGGAGCCG 
AACATCTACT CGCAGGTAAA TGTTATGATG GACGACGAGA TTCTGGCCGA CTTGCAGGCG 
GCCACAGCAT CCGGCAGAAG CTATGTGGCT GGAGCCGTGG TCGACAATGT GGGCGGAGCG 
CCCAATCTCT ACGTTCAAGG CTATGCGACG CGTATCGATC TGGCACATCA TCCCATGCCC 
ATGTACTCAA CGAGCCCGAT GTTTGGCACG AACAGCACCA TCACCGGAGT CACCATGTCG 
CATCCATCAC AATTGGCCAC CTTCTCGCCG ACGCCGCCGC CGCCCATCTT CACCCACAGT 
ATGGTGACCA CTGGCGATGG GCTCCTGCAG CCGGTGACGT GCATGGGCCT GGTGGCAACA 
TCTTCGGCAG GGGCATCGGT TCCTGGTCCT GTTCCACTGG CCCCGCAGCA ACAGCAACAG 
CAGCAGCTGA CAGCGGCCAA TGGCGGAAAC GGAAGCATCG TCGGCATGGG CATGAGCCTT 
TATGGCAGCG ATGTGGGCAA ACCACAGTTG CTAAAGACCG TACCGGAGGA GGTTCACCAT 
CAGCTGAACG GGGAGGATAT CCTGATCGCC CAGGATCGCA ATCTCGGATC GGGAACTCGC 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

side-VIII ( NM_001144248.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137720.1 Drosophila melanogaster sidestep VIII (side-VIII), transcript variant D, mRNA.

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CloneID	OFa13141
Clone ID Related Accession (Same CDS sequence)	NM_001144248.2
Accession Version	NM_001144248.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	sidestep VIII, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001144248.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCAGGA CCTCCAGCCG CTCGCTCTTC CTGCCGCTCT GCCTGCTCGT CCTGTCCGCG 
CTAAATGGCC CAGCGGCGGC GGCATCTCGC GTCCGCGTCA GCTCCTCGAC CACCATGACC 
AGGAACATCG AGGAGGAGAA TGGACCTCCC GTGCTATCTG AGTCCATAAT GGGTACGGTC 
GGCCGGCTGC CCTGCAACGT CACCCCGCCC ATTTACGAGG ATCGAGTGGC GCTGGTTATC 
TGGTACAAAG TTGGCCTGAA GACTCCCATT TACAGTGTGG ACACACGCGA CTCGAACTTC 
GCCCAGGGAA CGCACTGGTC GGATGAGACC TACCGGGAGC GGCTCTCCTT CCACGTGGAA 
GGACGTGCTG GCACGCTGAC CATCAAGTCA ACCACGGAGG ACGACACGGG CGAGTATCGC 
TGCCGCGTCG ACTTCCAGAA GAGTCCGACT CGCAACTCCA AAGTGAATTT AACTGTCATA 
ATTCCGCCCG AGTCGGTGAT TATATTGGAC AGCAAGGGTG TGACCATCGA GGATCACACG 
CTGGGTCCTT ACAACGAGGG CTCTGGGATA AACATCACGT GCGTGGCCAT CGGCGGCCGC 
CCGCAGCCGA GGGTTACCTG GCTGCATGGA AACACCGTCT ACAAGAATGC CAGTGTGGGC 
CAACCCCTGT CCGAGCGACG GGTGGGCAAT ACCCTGTCCT TGGCTCGACT GGAGCGCAGG 
AATCTTCACA TGCAGCTAAC GTGCCGGGCG GAGAACAATA ATTTGACAAC GCCAATTATC 
AGCAGCGTCG TCCTCGACAT GAACCTGCGT CCTTTGATTG TGAAGCTGCA GGGGGAGAAT 
CGAGCTCTGT CGGCGGGGAA TTCTTATCAG CTCAGTTGCG TTGTCATCGG AGCACGTCCA 
GCGCCGACGA TTACCTGGTG GAAGGGCAGT ACCCCCATGA AGAATACTCA CGAGATTGCT 
ACACCGGACG GTAATCTGAC TACCTCGGTG CTGACATTCA CGCCCACCAT CGATGACCGC 
GGCAAGTTCC TCTCGTGTCG CGCGGAGCAG AGCATGATAC CCGAGTCCGG AATGGAGGAC 
GGCTGGAAAT TGGACATATA TCACATACCG GTGGTGAGCC TGGAGCTGGG AACCAATTCG 
CTTAACTCAA CTCTGCGCGA GGGAATCGAT GTCTTCTTCG AGTGCAACAT CAAATCGAAT 
CCCTGGATAT ACGAAGTTAG CTGGCGGCAC AATGGTAAGA TTCTAACCAA CAATCCAGCC 
GAAGGCATCG CCGTCTCCAA CCAGAGTCTG GTGCTTCAAA ATGCCAGCCG GGCGAGAAGT 
GGCATCTACA CCTGTGTGGG CAGTAATCGG GAGGGCGACG GCGAGAGCAA TCCCGTTCAG 
CTGGACATAC GATTTGCTCC TGTTTGCCGA CCTCGCCAGC GTCTCTCCTA TAGCTCGGGC 
AGGCATGAGA CTGTCAAGGT GGCCTGCGAA ATTGATGCCA ATCCCGCGGA GGCCACCTAC 
GTTTGGAAAT TCAATGCAAC CCAGGGCGAA ACAGTTGACA TACCGGCTTC GCAAGTGGCC 
GTGGATCGGG GCCGAAGTAT AGCCCATTAC ACGCCCATGA CGGAGAATGA CTACGGTACG 
CTGCTCTGCT GGGCCACCAA CGAAATTGGC GATCAAAGTG AACCCTGCGT ATACACAATA 
TTCCCAGCAG GCGAGCCGGA TCCGTTGCTA AACTGCACGG TGCTCAACCA GACATCGACG 
GGCTTCCAAA TCGAATGCAT CGAGGGCTTC AATGGCGGCC TGCAGCAGGA CTTTATAATG 
GAGGTTTACA TGAACGGAAC GACACGACAT CCCAAAATTT CAAAATCAAA GCGACCGTAC 
TTTGAGGTAA GCGGACTGGT GCCCGGAATG GGCTACAATG TCTTCCTCAT TGCCAACAAC 
AGCAAGGGCC GTAGCAATGC CACCATTTTG CAGGTCTACA CGCTGAAGGA TCCGGAGAAG 
CAGACGGTTA AAATAACATT CACTAAGTCC TTCCAAGGAC TTAGGCCTGC CTATACCAAA 
ACAAAAGCGA CAGCGACCAC GACCAGCGAC TGCGTTTGCG ATGAGGATGA GTTCCTAAAT 
CTGGGCAAGG GAATTAGCCA CGACGAGGGC ACAGACGCTG ACCAGCAAGA AGACCTCTCG 
TTGGCCTATG CCCCCGTCAT CGAGGACATC CGGCCATTCC TCGGCATTCT GGCCGGCATC 
GTGGGCAGCA TTTTCCTGGT GGCCCTCATC ATTGTGATTG TGGTGCGTGT GCGCGGCTCT 
TCGGGGCGCG ATCGCAATAA TTACTCACAT CCGGGAAGCG GCGTCGGCGG CGTGGGCGGC 
GTGGGCGGCA CCACCGGCAA CGGCAGTGGG ATGGGCGGCG GTGGCGGCGG TGTGGGCGGC 
ACCACGGCCA ACGGGAATGG CAGTCTGGGG CTGCTGGCTA GCAACAACAA TGGCAGCCTC 
GTCATCGGCA CCCTGGGCCA CAATGGTGGG CAATCAGTGC AGGATATGCA CCGCATCGGC 
CGGGAGACGT GCCATGTGAC GAGCAGTCTG GACAGCATCG ACAAGAATCC GGACATCATA 
CCGCAAGACG GACACGACCT AGACGATGAG TGGACGACAA AGGGACATGG TCGCACTTAC 
GCCACGGCAG CGATGGCCGA GCAAAATGCC GTGATAACCT CCGGCACCTA TGATCACCTG 
ATGCCCACGT ACGCCGTTGT CGACAAGAAG ACAGCACCGC CTCCCGGCCA TGGCCAGTAC 
ATCCAGTATA ATACACTCAT TCCCGTTAGC AAAATGGGCG CTTATGCCAA TCAACAGCAG 
CAGCTCCAGC AGCAGCCGCA ACAGAAGACT GAGCTCAGTT ATAGCGAACT AAGCGCTCCT 
CTCGTCAGCG GCCCGGTGGG CGTTCAACGG ATGGCACCCT ACTGCAGCGC GACCTTGGGA 
CGGCCGGGCA GGCAGGCGGA ACTTAAGCGT GCGGAGCCGA ACATCTACTC GCAGGTAAAT 
GTTATGATGG ACGACGAGAT TCTGGCCGAC TTGCAGGCGG CCACAGCATC CGGCAGAAGC 
TATGTGGCTG GAGCCGTGGT CGACAATGTG GGCGGAGCGC CCAATCTCTA CGTTCAAGGC 
TATGCGACGC GTATCGATCT GGCACATCAT CCCATGCCCA TGTACTCAAC GAGCCCGATG 
TTTGGCACGA ACAGCACCAT CACCGGAGTC ACCATGTCGC ATCCATCACA ATTGGCCACC 
TTCTCGCCGA CGCCGCCGCC GCCCATCTTC ACCCACAGTA TGGTGACCAC TGGCGATGGG 
CTCCTGCAGC CGGTGACGTG CATGGGCCTG GTGGCAACAT CTTCGGCAGG GGCATCGGTT 
CCTGGTCCTG TTCCACTGGC CCCGCAGCAA CAGCAACAGC AGCAGCTGAC AGCGGCCAAT 
GGCGGAAACG GAAGCATCGT CGGCATGGGC ATGAGCCTTT ATGGCAGCGA TGTGGGCAAA 
CCACAGTTGC TAAAGACCGT ACCGGAGGAG GTTCACCATC AGCTGAACGG GGAGGATATC 
CTGATCGCCC AGGATCGCAA TCTCGGATCG GGAACTCGCT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137720.1
CDS	551..4135
Translation	MSRTSSRSLFLPLCLLVLSALNGPAAAASRVRVSSSTTMTRNIEEENGPPVLSESIMGTVGRLPCNVTPPIYEDRVALVIWYKVGLKTPIYSVDTRDSNFAQGTHWSDETYRERLSFHVEGRAGTLTIKSTTEDDTGEYRCRVDFQKSPTRNSKVNLTVIIPPESVIILDSKGVTIEDHTLGPYNEGSGINITCVAIGGRPQPRVTWLHGNTVYKNASVGQPLSERRVGNTLSLARLERRNLHMQLTCRAENNNLTTPIISSVVLDMNLRPLIVKLQGENRALSAGNSYQLSCVVIGARPAPTITWWKGSTPMKNTHEIATPDGNLTTSVLTFTPTIDDRGKFLSCRAEQSMIPESGMEDGWKLDIYHIPVVSLELGTNSLNSTLREGIDVFFECNIKSNPWIYEVSWRHNGKILTNNPAEGIAVSNQSLVLQNASRARSGIYTCVGSNREGDGESNPVQLDIRFAPVCRPRQRLSYSSGRHETVKVACEIDANPAEATYVWKFNATQGETVDIPASQVAVDRGRSIAHYTPMTENDYGTLLCWATNEIGDQSEPCVYTIFPAGEPDPLLNCTVLNQTSTGFQIECIEGFNGGLQQDFIMEVYMNGTTRHPKISKSKRPYFEVSGLVPGMGYNVFLIANNSKGRSNATILQVYTLKDPEKQTVKITFTKSFQGLRPAYTKTKATATTTSDCVCDEDEFLNLGKGISHDEGTDADQQEDLSLAYAPVIEDIRPFLGILAGIVGSIFLVALIIVIVVRVRGSSGRDRNNYSHPGSGVGGVGGVGGTTGNGSGMGGGGGGVGGTTANGNGSLGLLASNNNGSLVIGTLGHNGGQSVQDMHRIGRETCHVTSSLDSIDKNPDIIPQDGHDLDDEWTTKGHGRTYATAAMAEQNAVITSGTYDHLMPTYAVVDKKTAPPPGHGQYIQYNTLIPVSKMGAYANQQQQLQQQPQQKTELSYSELSAPLVSGPVGVQRMAPYCSATLGRPGRQAELKRAEPNIYSQVNVMMDDEILADLQAATASGRSYVAGAVVDNVGGAPNLYVQGYATRIDLAHHPMPMYSTSPMFGTNSTITGVTMSHPSQLATFSPTPPPPIFTHSMVTTGDGLLQPVTCMGLVATSSAGASVPGPVPLAPQQQQQQQLTAANGGNGSIVGMGMSLYGSDVGKPQLLKTVPEEVHHQLNGEDILIAQDRNLGSGTRF

Target ORF information:

RefSeq Version	NM_001144248.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster sidestep VIII (side-VIII), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144248.2

ORF Insert Sequence:

ATGAGCAGGA CCTCCAGCCG CTCGCTCTTC CTGCCGCTCT GCCTGCTCGT CCTGTCCGCG 
CTAAATGGCC CAGCGGCGGC GGCATCTCGC GTCCGCGTCA GCTCCTCGAC CACCATGACC 
AGGAACATCG AGGAGGAGAA TGGACCTCCC GTGCTATCTG AGTCCATAAT GGGTACGGTC 
GGCCGGCTGC CCTGCAACGT CACCCCGCCC ATTTACGAGG ATCGAGTGGC GCTGGTTATC 
TGGTACAAAG TTGGCCTGAA GACTCCCATT TACAGTGTGG ACACACGCGA CTCGAACTTC 
GCCCAGGGAA CGCACTGGTC GGATGAGACC TACCGGGAGC GGCTCTCCTT CCACGTGGAA 
GGACGTGCTG GCACGCTGAC CATCAAGTCA ACCACGGAGG ACGACACGGG CGAGTATCGC 
TGCCGCGTCG ACTTCCAGAA GAGTCCGACT CGCAACTCCA AAGTGAATTT AACTGTCATA 
ATTCCGCCCG AGTCGGTGAT TATATTGGAC AGCAAGGGTG TGACCATCGA GGATCACACG 
CTGGGTCCTT ACAACGAGGG CTCTGGGATA AACATCACGT GCGTGGCCAT CGGCGGCCGC 
CCGCAGCCGA GGGTTACCTG GCTGCATGGA AACACCGTCT ACAAGAATGC CAGTGTGGGC 
CAACCCCTGT CCGAGCGACG GGTGGGCAAT ACCCTGTCCT TGGCTCGACT GGAGCGCAGG 
AATCTTCACA TGCAGCTAAC GTGCCGGGCG GAGAACAATA ATTTGACAAC GCCAATTATC 
AGCAGCGTCG TCCTCGACAT GAACCTGCGT CCTTTGATTG TGAAGCTGCA GGGGGAGAAT 
CGAGCTCTGT CGGCGGGGAA TTCTTATCAG CTCAGTTGCG TTGTCATCGG AGCACGTCCA 
GCGCCGACGA TTACCTGGTG GAAGGGCAGT ACCCCCATGA AGAATACTCA CGAGATTGCT 
ACACCGGACG GTAATCTGAC TACCTCGGTG CTGACATTCA CGCCCACCAT CGATGACCGC 
GGCAAGTTCC TCTCGTGTCG CGCGGAGCAG AGCATGATAC CCGAGTCCGG AATGGAGGAC 
GGCTGGAAAT TGGACATATA TCACATACCG GTGGTGAGCC TGGAGCTGGG AACCAATTCG 
CTTAACTCAA CTCTGCGCGA GGGAATCGAT GTCTTCTTCG AGTGCAACAT CAAATCGAAT 
CCCTGGATAT ACGAAGTTAG CTGGCGGCAC AATGGTAAGA TTCTAACCAA CAATCCAGCC 
GAAGGCATCG CCGTCTCCAA CCAGAGTCTG GTGCTTCAAA ATGCCAGCCG GGCGAGAAGT 
GGCATCTACA CCTGTGTGGG CAGTAATCGG GAGGGCGACG GCGAGAGCAA TCCCGTTCAG 
CTGGACATAC GATTTGCTCC TGTTTGCCGA CCTCGCCAGC GTCTCTCCTA TAGCTCGGGC 
AGGCATGAGA CTGTCAAGGT GGCCTGCGAA ATTGATGCCA ATCCCGCGGA GGCCACCTAC 
GTTTGGAAAT TCAATGCAAC CCAGGGCGAA ACAGTTGACA TACCGGCTTC GCAAGTGGCC 
GTGGATCGGG GCCGAAGTAT AGCCCATTAC ACGCCCATGA CGGAGAATGA CTACGGTACG 
CTGCTCTGCT GGGCCACCAA CGAAATTGGC GATCAAAGTG AACCCTGCGT ATACACAATA 
TTCCCAGCAG GCGAGCCGGA TCCGTTGCTA AACTGCACGG TGCTCAACCA GACATCGACG 
GGCTTCCAAA TCGAATGCAT CGAGGGCTTC AATGGCGGCC TGCAGCAGGA CTTTATAATG 
GAGGTTTACA TGAACGGAAC GACACGACAT CCCAAAATTT CAAAATCAAA GCGACCGTAC 
TTTGAGGTAA GCGGACTGGT GCCCGGAATG GGCTACAATG TCTTCCTCAT TGCCAACAAC 
AGCAAGGGCC GTAGCAATGC CACCATTTTG CAGGTCTACA CGCTGAAGGA TCCGGAGAAG 
CAGACGGTTA AAATAACATT CACTAAGTCC TTCCAAGGAC TTAGGCCTGC CTATACCAAA 
ACAAAAGCGA CAGCGACCAC GACCAGCGAC TGCGTTTGCG ATGAGGATGA GTTCCTAAAT 
CTGGGCAAGG GAATTAGCCA CGACGAGGGC ACAGACGCTG ACCAGCAAGA AGACCTCTCG 
TTGGCCTATG CCCCCGTCAT CGAGGACATC CGGCCATTCC TCGGCATTCT GGCCGGCATC 
GTGGGCAGCA TTTTCCTGGT GGCCCTCATC ATTGTGATTG TGGTGCGTGT GCGCGGCTCT 
TCGGGGCGCG ATCGCAATAA TTACTCACAT CCGGGAAGCG GCGTCGGCGG CGTGGGCGGC 
GTGGGCGGCA CCACCGGCAA CGGCAGTGGG ATGGGCGGCG GTGGCGGCGG TGTGGGCGGC 
ACCACGGCCA ACGGGAATGG CAGTCTGGGG CTGCTGGCTA GCAACAACAA TGGCAGCCTC 
GTCATCGGCA CCCTGGGCCA CAATGGTGGG CAATCAGTGC AGGATATGCA CCGCATCGGC 
CGGGAGACGT GCCATGTGAC GAGCAGTCTG GACAGCATCG ACAAGAATCC GGACATCATA 
CCGCAAGACG GACACGACCT AGACGATGAG TGGACGACAA AGGGACATGG TCGCACTTAC 
GCCACGGCAG CGATGGCCGA GCAAAATGCC GTGATAACCT CCGGCACCTA TGATCACCTG 
ATGCCCACGT ACGCCGTTGT CGACAAGAAG ACAGCACCGC CTCCCGGCCA TGGCCAGTAC 
ATCCAGTATA ATACACTCAT TCCCGTTAGC AAAATGGGCG CTTATGCCAA TCAACAGCAG 
CAGCTCCAGC AGCAGCCGCA ACAGAAGACT GAGCTCAGTT ATAGCGAACT AAGCGCTCCT 
CTCGTCAGCG GCCCGGTGGG CGTTCAACGG ATGGCACCCT ACTGCAGCGC GACCTTGGGA 
CGGCCGGGCA GGCAGGCGGA ACTTAAGCGT GCGGAGCCGA ACATCTACTC GCAGGTAAAT 
GTTATGATGG ACGACGAGAT TCTGGCCGAC TTGCAGGCGG CCACAGCATC CGGCAGAAGC 
TATGTGGCTG GAGCCGTGGT CGACAATGTG GGCGGAGCGC CCAATCTCTA CGTTCAAGGC 
TATGCGACGC GTATCGATCT GGCACATCAT CCCATGCCCA TGTACTCAAC GAGCCCGATG 
TTTGGCACGA ACAGCACCAT CACCGGAGTC ACCATGTCGC ATCCATCACA ATTGGCCACC 
TTCTCGCCGA CGCCGCCGCC GCCCATCTTC ACCCACAGTA TGGTGACCAC TGGCGATGGG 
CTCCTGCAGC CGGTGACGTG CATGGGCCTG GTGGCAACAT CTTCGGCAGG GGCATCGGTT 
CCTGGTCCTG TTCCACTGGC CCCGCAGCAA CAGCAACAGC AGCAGCTGAC AGCGGCCAAT 
GGCGGAAACG GAAGCATCGT CGGCATGGGC ATGAGCCTTT ATGGCAGCGA TGTGGGCAAA 
CCACAGTTGC TAAAGACCGT ACCGGAGGAG GTTCACCATC AGCTGAACGG GGAGGATATC 
CTGATCGCCC AGGATCGCAA TCTCGGATCG GGAACTCGCT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

side-VIII ( NM_001103913.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097383.3 Drosophila melanogaster sidestep VIII (side-VIII), transcript variant E, mRNA.

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CloneID	OFa21876
Clone ID Related Accession (Same CDS sequence)	NM_001103913.3
Accession Version	NM_001103913.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	sidestep VIII, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001103913.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCAGGA CCTCCAGCCG CTCGCTCTTC CTGCCGCTCT GCCTGCTCGT CCTGTCCGCG 
CTAAATGGCC CAGCGGCGGC GGCATCTCGC GTCCGCGTCA GCTCCTCGAC CACCATGACC 
AGGAACATCG AGGAGGAGAA TGGACCTCCC GTGCTATCTG AGTCCATAAT GGGTACGGTC 
GGCCGGCTGC CCTGCAACGT CACCCCGCCC ATTTACGAGG ATCGAGTGGC GCTGGTTATC 
TGGTACAAAG TTGGCCTGAA GACTCCCATT TACAGTGTGG ACACACGCGA CTCGAACTTC 
GCCCAGGGAA CGCACTGGTC GGATGAGACC TACCGGGAGC GGCTCTCCTT CCACGTGGAA 
GGACGTGCTG GCACGCTGAC CATCAAGTCA ACCACGGAGG ACGACACGGG CGAGTATCGC 
TGCCGCGTCG ACTTCCAGAA GAGTCCGACT CGCAACTCCA AAGTGAATTT AACTGTCATA 
ATTCCGCCCG AGTCGGTGAT TATATTGGAC AGCAAGGGTG TGACCATCGA GGATCACACG 
CTGGGTCCTT ACAACGAGGG CTCTGGGATA AACATCACGT GCGTGGCCAT CGGCGGCCGC 
CCGCAGCCGA GGGTTACCTG GCTGCATGGA AACACCGTCT ACAAGAATGC CAGTGTGGGC 
CAACCCCTGT CCGAGCGACG GGTGGGCAAT ACCCTGTCCT TGGCTCGACT GGAGCGCAGG 
AATCTTCACA TGCAGCTAAC GTGCCGGGCG GAGAACAATA ATTTGACAAC GCCAATTATC 
AGCAGCGTCG TCCTCGACAT GAACCTGCGT CCTTTGATTG TGAAGCTGCA GGGGGAGAAT 
CGAGCTCTGT CGGCGGGGAA TTCTTATCAG CTCAGTTGCG TTGTCATCGG AGCACGTCCA 
GCGCCGACGA TTACCTGGTG GAAGGGCAGT ACCCCCATGA AGAATACTCA CGAGATTGCT 
ACACCGGACG GTAATCTGAC TACCTCGGTG CTGACATTCA CGCCCACCAT CGATGACCGC 
GGCAAGTTCC TCTCGTGTCG CGCGGAGCAG AGCATGATAC CCGAGTCCGG AATGGAGGAC 
GGCTGGAAAT TGGACATATA TCACATACCG GTGGTGAGCC TGGAGCTGGG AACCAATTCG 
CTTAACTCAA CTCTGCGCGA GGGAATCGAT GTCTTCTTCG AGTGCAACAT CAAATCGAAT 
CCCTGGATAT ACGAAGTTAG CTGGCGGCAC AATGGTAAGA TTCTAACCAA CAATCCAGCC 
GAAGGCATCG CCGTCTCCAA CCAGAGTCTG GTGCTTCAAA ATGCCAGCCG GGCGAGAAGT 
GGCATCTACA CCTGTGTGGG CAGTAATCGG GAGGGCGACG GCGAGAGCAA TCCCGTTCAG 
CTGGACATAC GATTTGCTCC TGTTTGCCGA CCTCGCCAGC GTCTCTCCTA TAGCTCGGGC 
AGGCATGAGA CTGTCAAGGT GGCCTGCGAA ATTGATGCCA ATCCCGCGGA GGCCACCTAC 
GTTTGGAAAT TCAATGCAAC CCAGGGCGAA ACAGTTGACA TACCGGCTTC GCAAGTGGCC 
GTGGATCGGG GCCGAAGTAT AGCCCATTAC ACGCCCATGA CGGAGAATGA CTACGGTACG 
CTGCTCTGCT GGGCCACCAA CGAAATTGGC GATCAAAGTG AACCCTGCGT ATACACAATA 
TTCCCAGCAG GCGAGCCGGA TCCGTTGCTA AACTGCACGG TGCTCAACCA GACATCGACG 
GGCTTCCAAA TCGAATGCAT CGAGGGCTTC AATGGCGGCC TGCAGCAGGA CTTTATAATG 
GAGGTTTACA TGAACGGAAC GACACGACAT CCCAAAATTT CAAAATCAAA GCGACCGTAC 
TTTGAGGTAA GCGGACTGGT GCCCGGAATG GGCTACAATG TCTTCCTCAT TGCCAACAAC 
AGCAAGGGCC GTAGCAATGC CACCATTTTG CAGGTCTACA CGCTGAAGGA TCCGGAGAAG 
CAGACGGACC TCTCGTTGGC CTATGCCCCC GTCATCGAGG ACATCCGGCC ATTCCTCGGC 
ATTCTGGCCG GCATCGTGGG CAGCATTTTC CTGGTGGCCC TCATCATTGT GATTGTGGTG 
CGTGTGCGCG GCTCTTCGGG GCGCGATCGC AATAATTACT CACATCCGGG AAGCGGCGTC 
GGCGGCGTGG GCGGCGTGGG CGGCACCACC GGCAACGGCA GTGGGATGGG CGGCGGTGGC 
GGCGGTGTGG GCGGCACCAC GGCCAACGGG AATGGCAGTC TGGGGCTGCT GGCTAGCAAC 
AACAATGGCA GCCTCGTCAT CGGCACCCTG GGCCACAATG GTGGGCAATC AGTGCAGGAT 
ATGCACCGCA TCGGCCGGGA GACGTGCCAT GTGACGAGCA GTCTGGACAG CATCGACAAG 
AATCCGGACA TCATACCGCA AGGTGGGTTG GACGGACACG ACCTAGACGA TGAGTGGACG 
ACAAAGGGAC ATGGTCGCAC TTACGCCACG GCAGCGATGG CCGAGCAAAA TGCCGTGATA 
ACCTCCGGCA CCTATGATCA CCTGATGCCC ACGTACGCCG TTGTCGACAA GAAGACAGCA 
CCGCCTCCCG GCCATGGCCA GTACATCCAG TATAATACAC TCATTCCCGT TAGCAAAATG 
GGCGCTTATG CCAATCAACA GCAGCAGCTC CAGCAGCAGC CGCAACAGAA GACTGAGCTC 
AGTTATAGCG AACTAAGCGC TCCTCTCGTC AGCGGCCCGG TGGGCGTTCA ACGGATGGCA 
CCCTACTGCA GCGCGACCTT GGGACGGCCG GGCAGGCAGG CGGAACTTAA GCGTGCGGAG 
CCGAACATCT ACTCGCAGGT AAATGTTATG ATGGACGACG AGATTCTGGC CGACTTGCAG 
GCGGCCACAG CATCCGGCAG AAGCTATGTG GCTGGAGCCG TGGTCGACAA TGTGGGCGGA 
GCGCCCAATC TCTACGTTCA AGGCTATGCG ACGCGTATCG ATCTGGCACA TCATCCCATG 
CCCATGTACT CAACGAGCCC GATGTTTGGC ACGAACAGCA CCATCACCGG AGTCACCATG 
TCGCATCCAT CACAATTGGC CACCTTCTCG CCGACGCCGC CGCCGCCCAT CTTCACCCAC 
AGTATGGTGA CCACTGGCGA TGGGCTCCTG CAGCCGGTGA CGTGCATGGG CCTGGTGGCA 
ACATCTTCGG CAGGGGCATC GGTTCCTGGT CCTGTTCCAC TGGCCCCGCA GCAACAGCAA 
CAGCAGCAGC TGACAGCGGC CAATGGCGGA AACGGAAGCA TCGTCGGCAT GGGCATGAGC 
CTTTATGGCA GCGATGTGGG CAAACCACAG TTGCTAAAGA CCGTACCGGA GGAGGTTCAC 
CATCAGCTGA ACGGGGAGGA TATCCTGATC GCCCAGGATC GCAATCTCGG ATCGGGAACT 
CGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097383.3
CDS	551..3979
Translation	MSRTSSRSLFLPLCLLVLSALNGPAAAASRVRVSSSTTMTRNIEEENGPPVLSESIMGTVGRLPCNVTPPIYEDRVALVIWYKVGLKTPIYSVDTRDSNFAQGTHWSDETYRERLSFHVEGRAGTLTIKSTTEDDTGEYRCRVDFQKSPTRNSKVNLTVIIPPESVIILDSKGVTIEDHTLGPYNEGSGINITCVAIGGRPQPRVTWLHGNTVYKNASVGQPLSERRVGNTLSLARLERRNLHMQLTCRAENNNLTTPIISSVVLDMNLRPLIVKLQGENRALSAGNSYQLSCVVIGARPAPTITWWKGSTPMKNTHEIATPDGNLTTSVLTFTPTIDDRGKFLSCRAEQSMIPESGMEDGWKLDIYHIPVVSLELGTNSLNSTLREGIDVFFECNIKSNPWIYEVSWRHNGKILTNNPAEGIAVSNQSLVLQNASRARSGIYTCVGSNREGDGESNPVQLDIRFAPVCRPRQRLSYSSGRHETVKVACEIDANPAEATYVWKFNATQGETVDIPASQVAVDRGRSIAHYTPMTENDYGTLLCWATNEIGDQSEPCVYTIFPAGEPDPLLNCTVLNQTSTGFQIECIEGFNGGLQQDFIMEVYMNGTTRHPKISKSKRPYFEVSGLVPGMGYNVFLIANNSKGRSNATILQVYTLKDPEKQTDLSLAYAPVIEDIRPFLGILAGIVGSIFLVALIIVIVVRVRGSSGRDRNNYSHPGSGVGGVGGVGGTTGNGSGMGGGGGGVGGTTANGNGSLGLLASNNNGSLVIGTLGHNGGQSVQDMHRIGRETCHVTSSLDSIDKNPDIIPQGGLDGHDLDDEWTTKGHGRTYATAAMAEQNAVITSGTYDHLMPTYAVVDKKTAPPPGHGQYIQYNTLIPVSKMGAYANQQQQLQQQPQQKTELSYSELSAPLVSGPVGVQRMAPYCSATLGRPGRQAELKRAEPNIYSQVNVMMDDEILADLQAATASGRSYVAGAVVDNVGGAPNLYVQGYATRIDLAHHPMPMYSTSPMFGTNSTITGVTMSHPSQLATFSPTPPPPIFTHSMVTTGDGLLQPVTCMGLVATSSAGASVPGPVPLAPQQQQQQQLTAANGGNGSIVGMGMSLYGSDVGKPQLLKTVPEEVHHQLNGEDILIAQDRNLGSGTRF

Target ORF information:

RefSeq Version	NM_001103913.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster sidestep VIII (side-VIII), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103913.3

ORF Insert Sequence:

ATGAGCAGGA CCTCCAGCCG CTCGCTCTTC CTGCCGCTCT GCCTGCTCGT CCTGTCCGCG 
CTAAATGGCC CAGCGGCGGC GGCATCTCGC GTCCGCGTCA GCTCCTCGAC CACCATGACC 
AGGAACATCG AGGAGGAGAA TGGACCTCCC GTGCTATCTG AGTCCATAAT GGGTACGGTC 
GGCCGGCTGC CCTGCAACGT CACCCCGCCC ATTTACGAGG ATCGAGTGGC GCTGGTTATC 
TGGTACAAAG TTGGCCTGAA GACTCCCATT TACAGTGTGG ACACACGCGA CTCGAACTTC 
GCCCAGGGAA CGCACTGGTC GGATGAGACC TACCGGGAGC GGCTCTCCTT CCACGTGGAA 
GGACGTGCTG GCACGCTGAC CATCAAGTCA ACCACGGAGG ACGACACGGG CGAGTATCGC 
TGCCGCGTCG ACTTCCAGAA GAGTCCGACT CGCAACTCCA AAGTGAATTT AACTGTCATA 
ATTCCGCCCG AGTCGGTGAT TATATTGGAC AGCAAGGGTG TGACCATCGA GGATCACACG 
CTGGGTCCTT ACAACGAGGG CTCTGGGATA AACATCACGT GCGTGGCCAT CGGCGGCCGC 
CCGCAGCCGA GGGTTACCTG GCTGCATGGA AACACCGTCT ACAAGAATGC CAGTGTGGGC 
CAACCCCTGT CCGAGCGACG GGTGGGCAAT ACCCTGTCCT TGGCTCGACT GGAGCGCAGG 
AATCTTCACA TGCAGCTAAC GTGCCGGGCG GAGAACAATA ATTTGACAAC GCCAATTATC 
AGCAGCGTCG TCCTCGACAT GAACCTGCGT CCTTTGATTG TGAAGCTGCA GGGGGAGAAT 
CGAGCTCTGT CGGCGGGGAA TTCTTATCAG CTCAGTTGCG TTGTCATCGG AGCACGTCCA 
GCGCCGACGA TTACCTGGTG GAAGGGCAGT ACCCCCATGA AGAATACTCA CGAGATTGCT 
ACACCGGACG GTAATCTGAC TACCTCGGTG CTGACATTCA CGCCCACCAT CGATGACCGC 
GGCAAGTTCC TCTCGTGTCG CGCGGAGCAG AGCATGATAC CCGAGTCCGG AATGGAGGAC 
GGCTGGAAAT TGGACATATA TCACATACCG GTGGTGAGCC TGGAGCTGGG AACCAATTCG 
CTTAACTCAA CTCTGCGCGA GGGAATCGAT GTCTTCTTCG AGTGCAACAT CAAATCGAAT 
CCCTGGATAT ACGAAGTTAG CTGGCGGCAC AATGGTAAGA TTCTAACCAA CAATCCAGCC 
GAAGGCATCG CCGTCTCCAA CCAGAGTCTG GTGCTTCAAA ATGCCAGCCG GGCGAGAAGT 
GGCATCTACA CCTGTGTGGG CAGTAATCGG GAGGGCGACG GCGAGAGCAA TCCCGTTCAG 
CTGGACATAC GATTTGCTCC TGTTTGCCGA CCTCGCCAGC GTCTCTCCTA TAGCTCGGGC 
AGGCATGAGA CTGTCAAGGT GGCCTGCGAA ATTGATGCCA ATCCCGCGGA GGCCACCTAC 
GTTTGGAAAT TCAATGCAAC CCAGGGCGAA ACAGTTGACA TACCGGCTTC GCAAGTGGCC 
GTGGATCGGG GCCGAAGTAT AGCCCATTAC ACGCCCATGA CGGAGAATGA CTACGGTACG 
CTGCTCTGCT GGGCCACCAA CGAAATTGGC GATCAAAGTG AACCCTGCGT ATACACAATA 
TTCCCAGCAG GCGAGCCGGA TCCGTTGCTA AACTGCACGG TGCTCAACCA GACATCGACG 
GGCTTCCAAA TCGAATGCAT CGAGGGCTTC AATGGCGGCC TGCAGCAGGA CTTTATAATG 
GAGGTTTACA TGAACGGAAC GACACGACAT CCCAAAATTT CAAAATCAAA GCGACCGTAC 
TTTGAGGTAA GCGGACTGGT GCCCGGAATG GGCTACAATG TCTTCCTCAT TGCCAACAAC 
AGCAAGGGCC GTAGCAATGC CACCATTTTG CAGGTCTACA CGCTGAAGGA TCCGGAGAAG 
CAGACGGACC TCTCGTTGGC CTATGCCCCC GTCATCGAGG ACATCCGGCC ATTCCTCGGC 
ATTCTGGCCG GCATCGTGGG CAGCATTTTC CTGGTGGCCC TCATCATTGT GATTGTGGTG 
CGTGTGCGCG GCTCTTCGGG GCGCGATCGC AATAATTACT CACATCCGGG AAGCGGCGTC 
GGCGGCGTGG GCGGCGTGGG CGGCACCACC GGCAACGGCA GTGGGATGGG CGGCGGTGGC 
GGCGGTGTGG GCGGCACCAC GGCCAACGGG AATGGCAGTC TGGGGCTGCT GGCTAGCAAC 
AACAATGGCA GCCTCGTCAT CGGCACCCTG GGCCACAATG GTGGGCAATC AGTGCAGGAT 
ATGCACCGCA TCGGCCGGGA GACGTGCCAT GTGACGAGCA GTCTGGACAG CATCGACAAG 
AATCCGGACA TCATACCGCA AGGTGGGTTG GACGGACACG ACCTAGACGA TGAGTGGACG 
ACAAAGGGAC ATGGTCGCAC TTACGCCACG GCAGCGATGG CCGAGCAAAA TGCCGTGATA 
ACCTCCGGCA CCTATGATCA CCTGATGCCC ACGTACGCCG TTGTCGACAA GAAGACAGCA 
CCGCCTCCCG GCCATGGCCA GTACATCCAG TATAATACAC TCATTCCCGT TAGCAAAATG 
GGCGCTTATG CCAATCAACA GCAGCAGCTC CAGCAGCAGC CGCAACAGAA GACTGAGCTC 
AGTTATAGCG AACTAAGCGC TCCTCTCGTC AGCGGCCCGG TGGGCGTTCA ACGGATGGCA 
CCCTACTGCA GCGCGACCTT GGGACGGCCG GGCAGGCAGG CGGAACTTAA GCGTGCGGAG 
CCGAACATCT ACTCGCAGGT AAATGTTATG ATGGACGACG AGATTCTGGC CGACTTGCAG 
GCGGCCACAG CATCCGGCAG AAGCTATGTG GCTGGAGCCG TGGTCGACAA TGTGGGCGGA 
GCGCCCAATC TCTACGTTCA AGGCTATGCG ACGCGTATCG ATCTGGCACA TCATCCCATG 
CCCATGTACT CAACGAGCCC GATGTTTGGC ACGAACAGCA CCATCACCGG AGTCACCATG 
TCGCATCCAT CACAATTGGC CACCTTCTCG CCGACGCCGC CGCCGCCCAT CTTCACCCAC 
AGTATGGTGA CCACTGGCGA TGGGCTCCTG CAGCCGGTGA CGTGCATGGG CCTGGTGGCA 
ACATCTTCGG CAGGGGCATC GGTTCCTGGT CCTGTTCCAC TGGCCCCGCA GCAACAGCAA 
CAGCAGCAGC TGACAGCGGC CAATGGCGGA AACGGAAGCA TCGTCGGCAT GGGCATGAGC 
CTTTATGGCA GCGATGTGGG CAAACCACAG TTGCTAAAGA CCGTACCGGA GGAGGTTCAC 
CATCAGCTGA ACGGGGAGGA TATCCTGATC GCCCAGGATC GCAATCTCGG ATCGGGAACT 
CGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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side-VIII cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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