5-HT1A cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	5-HT1A
Entrez Gene ID	37196
Full Name	5-hydroxytryptamine (serotonin) receptor 1A
Synonyms	5-HT-dro2A,5-HT1ADro,5-HT[1A]Dro,5-HT[[1ADro]],5-HT[[1A]],5-HT[[1A]]DRO,5HT-R2A,5HT-dro2A,5HT-drp[[2A]],5HT1A,5HT[[1ADro]],5htdro2a,CG16720,DRO2A,Dm5HTdro2A,Dmel\CG16720,HT1A,d5-HT1A
General protein information	Preferred Names 5-hydroxytryptamine (serotonin) receptor 1A Names CG16720 gene product from transcript CG16720-RB 5-HT1A-PB CG16720-PB serotonin receptor 1A serotonin receptor 2A serotonin-receptor-2A
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 56B2-56B5

mRNA and Protein(s)

mRNA	Protein	Name
NM_166322.2	NP_725849.1	5-hydroxytryptamine (serotonin) receptor 1A, isoform B
NM_001369983.1	NP_001356890.1	5-hydroxytryptamine (serotonin) receptor 1A, isoform C

Pathways from BioSystems

Homologies

Homo sapiens (human)	HTR1A	NP_000515.2
Mus musculus (house mouse)	Htr1a	NP_032334.2
Gallus gallus (chicken)	HTR1A	NP_001163999.1
Pan troglodytes (chimpanzee)	HTR1A	NP_001129094.1
Canis lupus familiaris (dog)	HTR1A	NP_001012397.1
Anopheles gambiae (African malaria mosquito)	GPR5HT1A	XP_308623.4
Xenopus tropicalis (tropical clawed frog)	htr1a	XP_004910432.1
Bos taurus (cattle)	HTR1A	XP_002696350.1
Danio rerio (zebrafish)	htr1aa	NP_001116793.1
Drosophila melanogaster (fruit fly)	5-HT1A	NP_725849.1
Caenorhabditis elegans (roundworm)	ser-4	NP_497452.1
Rattus norvegicus (Norway rat)	Htr1a	NP_036717.1
	HTR1A	XP_001083407.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Activation of 5-HT1A and OAMB signaling in Insulin-producing cells generates differential effects on Dilp transcription, fly physiology, metabolism and social interactions.
Title: Drosophila insulin-producing cells are differentially modulated by serotonin and octopamine receptors and affect social behavior.

serotonin acts on brain IPCs via the 5-HT(1A) receptor, thereby affecting their activity and probably insulin signaling
Title: Insulin-producing cells in the brain of adult Drosophila are regulated by the serotonin 5-HT1A receptor.

study describes the influence of different 5-HT receptor subtypes on aggressive behaviors; activation of 5-HT(1A)-like receptors with 8-OH-DPAT increases overall aggression; 5-HT(1A)-like receptor manipulation primarily affects wing threats and fencing
Title: Serotonin 5-HT(2) and 5-HT(1A)-like receptors differentially modulate aggressive behaviors in Drosophila melanogaster.

The Drosophila serotonin receptor 1A has a role in promoting baseline sleep.
Title: A sleep-promoting role for the Drosophila serotonin receptor 1A.

Decrease in 5-HT(1A) receptor binding partly reflects hippocampal neuronal loss in temporal lobe epilepsy.
Title: Statistical parametric mapping of 5-HT1A receptor binding in temporal lobe epilepsy with hippocampal ictal onset on intracranial EEG.

The following 5-HT1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the 5-HT1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa87722	NM_001369983.1 Latest version!	Drosophila melanogaster 5-hydroxytryptamine (serotonin) receptor 1A (5-HT1A), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OFa10570	NM_166322.2 Latest version!	Drosophila melanogaster 5-hydroxytryptamine (serotonin) receptor 1A (5-HT1A), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

5-HT1A ( NM_001369983.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001356890.1 Drosophila melanogaster 5-hydroxytryptamine (serotonin) receptor 1A (5-HT1A), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa87722
Clone ID Related Accession (Same CDS sequence)	NM_001369983.1
Accession Version	NM_001369983.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2541bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	5-hydroxytryptamine (serotonin) receptor 1A, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGCACG AGACCAGCTT TAATGATGCG CTTGACTACA TTTACATAGC GAACAGCATG 
AATGACAGGG CCTTTCTGAT AGCCGAACCG CATCCCGAAC AGCCGAATGT AGATGGTCAG 
GACCAGGACG ACGCGGAGCT GGAGGAACTG GACGACATGG CGGTCACGGA CGATGGACAG 
CTGGAGGATA CCAATAACAA CAACAACAGC AAGCGCTACT ACAGCTCCGG TAAAAGACGA 
GCGGATTTCA TAGGCTCCCT GGCGCTGAAG CCACCGCCCA CCGATGTGAA CACCACCACC 
ACCACCGCCG GCTCACCACT GGCCACCGCC GCTTTGGCAG CGGCAGCCGC CAGTGCCTCG 
GTGGCGGCAG CGGCTGCCAG GATCACCGCC AAGGCGGCAC ACAGGGCACT GACCACCAAG 
CAGGATGCCA CATCCTCGCC AGCATCCTCT CCAGCCCTCC AACTGATCGA CATGGACAAT 
AACTACACGA ATGTCGCAGT CGGACTGGGT GCCATGCTGC TAAATGACAC GCTCCTCCTC 
GAAGGCAACG ATTCCAGTTT GTTCGGCGAG ATGCTTGCCA ACCGGAGTGG CCAACTGGAT 
CTGATCAACG GCACTGGCGG CCTCAATGTG ACCACCAGCA AAGTGGCCGA GGACGACTTT 
ACGCAGCTGC TGCGAATGGC CGTCACATCG GTGCTGCTCG GTCTAATGAT ACTGGTCACC 
ATAATTGGCA ATGTCTTTGT CATAGCGGCC ATTATCCTGG AGCGGAATCT ACAAAACGTG 
GCCAACTACC TGGTGGCCTC ATTGGCAGTG GCCGATCTCT TCGTGGCCTG CCTAGTAATG 
CCCCTAGGAG CTGTATACGA GATCAGCCAA GGCTGGATAC TTGGTCCGGA ATTGTGCGAT 
ATCTGGACGT CGTGCGATGT CCTCTGCTGC ACTGCCTCGA TTTTGCATCT GGTGGCCATT 
GCCGTCGATC GTTACTGGGC GGTCACCAAT ATAGATTACA TCCACTCGCG GACCAGCAAT 
CGTGTCTTTA TGATGATCTT TTGCGTTTGG ACGGCGGCGG TGATTGTGTC CTTGGCACCA 
CAGTTCGGTT GGAAGGATCC AGACTATCTG CAGCGTATCG AGCAGCAAAA GTGCATGGTC 
TCGCAGGATG TTTCTTATCA GGTATTTGCC ACCTGTTGCA CATTTTACGT GCCACTGCTG 
GTCATCCTGG CGTTGTACTG GAAAATCTAT CAAACGGCCC GCAAACGCAT CCATCGTCGA 
CGACCGCGAC CCGTCGATGC GGCGGTCAAT AATAATCAGC CGGACGGAGG TGCGGCAACA 
GATACGAAAC TTCATCGACT GCGTCTACGC CTCGGCAGAT TCTCCACGGC CAAATCCAAA 
ACCGGAAGTG CCGTGGGCGT CTCAGGACCC GCATCCGGAG GCAGGGCTCT GGGCCTCGTC 
GATGGCAACT CAACGAACAC GGTCAACACC GTCGAGGACA CCGAGTTCAG CAGCTCGAAT 
GTGGACAGCA AAAGTCGGGC AGGCGTCGAG GCACCCAGCA CTTCTGGCAA CCAGATAGCC 
ACCGTATCGC ACCTGGTTGC GCTGGCCAAG CAGCAGGGCA AGTCCACCGC CAAATCCTCA 
GCCGCTGTCA ACGGAATGGC CCCATCGGGC AGGCAAGAGG ACGATGGCCA GCGACCAGAA 
CACGGGGAGC AGGAGGATCG CGAGGAGCTA GAGGACCAGG ACGAGCAGGT TGGCCCGCAG 
CCCACAACCG CAACATCAGC AATGACAGCG GCAGGCACCA ACGAGTCGGA GGACCAATGC 
AAGGCCAACG GGGTGGAGGT CCTGGAGGAT CCGCAGTTGC AACAGCAGCT CGAACAAGTG 
CAGCAGCTGC AGAAATCGGT TAAATCCGGC GGTGGAGGTG GGGCCAGTAC GTCAAATGCC 
ACCACAATTA CGTCGATATC AGCGCTATCG CCACAAACGC CAACATCGCA GGGAGTTGGA 
ATCGCCGCCG CCGCCGCTGG ACCGATGACG GCCAAAACCA GCACGCTGAC GAGCTGCAAC 
CAGTCGCATC CGCTATGCGG CACCGCCAAC GAATCCCCAT CGACACCAGA GCCACGTAGC 
CGACAACCGA CGACCCCGCA GCAGCAGCCG CATCAGCAGG CGCACCAGCA GCAGCAGCAG 
CAGCAGCAAC TCTCCAGCAT CGCCAATCCG ATGCAGAAGG TGAACAAGCG AAAGGAGACC 
CTGGAGGCCA AAAGGGAACG GAAGGCGGCC AAGACGCTGG CCATCATTAC CGGCGCCTTC 
GTCGTCTGCT GGCTGCCCTT CTTCGTCATG GCACTGACCA TGCCGCTCTG TGCCGCCTGC 
CAGATCAGCG ATTCCGTTGC CTCGCTCTTC CTTTGGCTGG GCTACTTCAA CTCCACCCTC 
AATCCGGTTA TTTACACCAT CTTCAGTCCG GAGTTCCGAC AGGCCTTCAA GCGCATCCTC 
TTCGGCGGCC ACCGACCAGT CCACTACCGC AGCGGGAAGC TCTAGATAGT GCATGCGAAG 
AGGCGAAGAC GCTTCTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001356890.1
CDS	1057..3597
Translation	MAHETSFNDALDYIYIANSMNDRAFLIAEPHPEQPNVDGQDQDDAELEELDDMAVTDDGQLEDTNNNNNSKRYYSSGKRRADFIGSLALKPPPTDVNTTTTTAGSPLATAALAAAAASASVAAAAARITAKAAHRALTTKQDATSSPASSPALQLIDMDNNYTNVAVGLGAMLLNDTLLLEGNDSSLFGEMLANRSGQLDLINGTGGLNVTTSKVAEDDFTQLLRMAVTSVLLGLMILVTIIGNVFVIAAIILERNLQNVANYLVASLAVADLFVACLVMPLGAVYEISQGWILGPELCDIWTSCDVLCCTASILHLVAIAVDRYWAVTNIDYIHSRTSNRVFMMIFCVWTAAVIVSLAPQFGWKDPDYLQRIEQQKCMVSQDVSYQVFATCCTFYVPLLVILALYWKIYQTARKRIHRRRPRPVDAAVNNNQPDGGAATDTKLHRLRLRLGRFSTAKSKTGSAVGVSGPASGGRALGLVDGNSTNTVNTVEDTEFSSSNVDSKSRAGVEAPSTSGNQIATVSHLVALAKQQGKSTAKSSAAVNGMAPSGRQEDDGQRPEHGEQEDREELEDQDEQVGPQPTTATSAMTAAGTNESEDQCKANGVEVLEDPQLQQQLEQVQQLQKSVKSGGGGGASTSNATTITSISALSPQTPTSQGVGIAAAAAGPMTAKTSTLTSCNQSHPLCGTANESPSTPEPRSRQPTTPQQQPHQQAHQQQQQQQQLSSIANPMQKVNKRKETLEAKRERKAAKTLAIITGAFVVCWLPFFVMALTMPLCAACQISDSVASLFLWLGYFNSTLNPVIYTIFSPEFRQAFKRILFGGHRPVHYRSGKL*IVHAKRRRRFC

Target ORF information:

RefSeq Version	NM_001369983.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster 5-hydroxytryptamine (serotonin) receptor 1A (5-HT1A), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001369983.1

ORF Insert Sequence:

ATGGCGCACG AGACCAGCTT TAATGATGCG CTTGACTACA TTTACATAGC GAACAGCATG 
AATGACAGGG CCTTTCTGAT AGCCGAACCG CATCCCGAAC AGCCGAATGT AGATGGTCAG 
GACCAGGACG ACGCGGAGCT GGAGGAACTG GACGACATGG CGGTCACGGA CGATGGACAG 
CTGGAGGATA CCAATAACAA CAACAACAGC AAGCGCTACT ACAGCTCCGG TAAAAGACGA 
GCGGATTTCA TAGGCTCCCT GGCGCTGAAG CCACCGCCCA CCGATGTGAA CACCACCACC 
ACCACCGCCG GCTCACCACT GGCCACCGCC GCTTTGGCAG CGGCAGCCGC CAGTGCCTCG 
GTGGCGGCAG CGGCTGCCAG GATCACCGCC AAGGCGGCAC ACAGGGCACT GACCACCAAG 
CAGGATGCCA CATCCTCGCC AGCATCCTCT CCAGCCCTCC AACTGATCGA CATGGACAAT 
AACTACACGA ATGTCGCAGT CGGACTGGGT GCCATGCTGC TAAATGACAC GCTCCTCCTC 
GAAGGCAACG ATTCCAGTTT GTTCGGCGAG ATGCTTGCCA ACCGGAGTGG CCAACTGGAT 
CTGATCAACG GCACTGGCGG CCTCAATGTG ACCACCAGCA AAGTGGCCGA GGACGACTTT 
ACGCAGCTGC TGCGAATGGC CGTCACATCG GTGCTGCTCG GTCTAATGAT ACTGGTCACC 
ATAATTGGCA ATGTCTTTGT CATAGCGGCC ATTATCCTGG AGCGGAATCT ACAAAACGTG 
GCCAACTACC TGGTGGCCTC ATTGGCAGTG GCCGATCTCT TCGTGGCCTG CCTAGTAATG 
CCCCTAGGAG CTGTATACGA GATCAGCCAA GGCTGGATAC TTGGTCCGGA ATTGTGCGAT 
ATCTGGACGT CGTGCGATGT CCTCTGCTGC ACTGCCTCGA TTTTGCATCT GGTGGCCATT 
GCCGTCGATC GTTACTGGGC GGTCACCAAT ATAGATTACA TCCACTCGCG GACCAGCAAT 
CGTGTCTTTA TGATGATCTT TTGCGTTTGG ACGGCGGCGG TGATTGTGTC CTTGGCACCA 
CAGTTCGGTT GGAAGGATCC AGACTATCTG CAGCGTATCG AGCAGCAAAA GTGCATGGTC 
TCGCAGGATG TTTCTTATCA GGTATTTGCC ACCTGTTGCA CATTTTACGT GCCACTGCTG 
GTCATCCTGG CGTTGTACTG GAAAATCTAT CAAACGGCCC GCAAACGCAT CCATCGTCGA 
CGACCGCGAC CCGTCGATGC GGCGGTCAAT AATAATCAGC CGGACGGAGG TGCGGCAACA 
GATACGAAAC TTCATCGACT GCGTCTACGC CTCGGCAGAT TCTCCACGGC CAAATCCAAA 
ACCGGAAGTG CCGTGGGCGT CTCAGGACCC GCATCCGGAG GCAGGGCTCT GGGCCTCGTC 
GATGGCAACT CAACGAACAC GGTCAACACC GTCGAGGACA CCGAGTTCAG CAGCTCGAAT 
GTGGACAGCA AAAGTCGGGC AGGCGTCGAG GCACCCAGCA CTTCTGGCAA CCAGATAGCC 
ACCGTATCGC ACCTGGTTGC GCTGGCCAAG CAGCAGGGCA AGTCCACCGC CAAATCCTCA 
GCCGCTGTCA ACGGAATGGC CCCATCGGGC AGGCAAGAGG ACGATGGCCA GCGACCAGAA 
CACGGGGAGC AGGAGGATCG CGAGGAGCTA GAGGACCAGG ACGAGCAGGT TGGCCCGCAG 
CCCACAACCG CAACATCAGC AATGACAGCG GCAGGCACCA ACGAGTCGGA GGACCAATGC 
AAGGCCAACG GGGTGGAGGT CCTGGAGGAT CCGCAGTTGC AACAGCAGCT CGAACAAGTG 
CAGCAGCTGC AGAAATCGGT TAAATCCGGC GGTGGAGGTG GGGCCAGTAC GTCAAATGCC 
ACCACAATTA CGTCGATATC AGCGCTATCG CCACAAACGC CAACATCGCA GGGAGTTGGA 
ATCGCCGCCG CCGCCGCTGG ACCGATGACG GCCAAAACCA GCACGCTGAC GAGCTGCAAC 
CAGTCGCATC CGCTATGCGG CACCGCCAAC GAATCCCCAT CGACACCAGA GCCACGTAGC 
CGACAACCGA CGACCCCGCA GCAGCAGCCG CATCAGCAGG CGCACCAGCA GCAGCAGCAG 
CAGCAGCAAC TCTCCAGCAT CGCCAATCCG ATGCAGAAGG TGAACAAGCG AAAGGAGACC 
CTGGAGGCCA AAAGGGAACG GAAGGCGGCC AAGACGCTGG CCATCATTAC CGGCGCCTTC 
GTCGTCTGCT GGCTGCCCTT CTTCGTCATG GCACTGACCA TGCCGCTCTG TGCCGCCTGC 
CAGATCAGCG ATTCCGTTGC CTCGCTCTTC CTTTGGCTGG GCTACTTCAA CTCCACCCTC 
AATCCGGTTA TTTACACCAT CTTCAGTCCG GAGTTCCGAC AGGCCTTCAA GCGCATCCTC 
TTCGGCGGCC ACCGACCAGT CCACTACCGC AGCGGGAAGC TCTAGATAGT GCATGCGAAG 
AGGCGAAGAC GCTTCTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

5-HT1A ( NM_166322.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725849.1 Drosophila melanogaster 5-hydroxytryptamine (serotonin) receptor 1A (5-HT1A), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa10570
Clone ID Related Accession (Same CDS sequence)	NM_166322.2
Accession Version	NM_166322.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2505bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	5-hydroxytryptamine (serotonin) receptor 1A, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_166322.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGCACG AGACCAGCTT TAATGATGCG CTTGACTACA TTTACATAGC GAACAGCATG 
AATGACAGGG CCTTTCTGAT AGCCGAACCG CATCCCGAAC AGCCGAATGT AGATGGTCAG 
GACCAGGACG ACGCGGAGCT GGAGGAACTG GACGACATGG CGGTCACGGA CGATGGACAG 
CTGGAGGATA CCAATAACAA CAACAACAGC AAGCGCTACT ACAGCTCCGG TAAAAGACGA 
GCGGATTTCA TAGGCTCCCT GGCGCTGAAG CCACCGCCCA CCGATGTGAA CACCACCACC 
ACCACCGCCG GCTCACCACT GGCCACCGCC GCTTTGGCAG CGGCAGCCGC CAGTGCCTCG 
GTGGCGGCAG CGGCTGCCAG GATCACCGCC AAGGCGGCAC ACAGGGCACT GACCACCAAG 
CAGGATGCCA CATCCTCGCC AGCATCCTCT CCAGCCCTCC AACTGATCGA CATGGACAAT 
AACTACACGA ATGTCGCAGT CGGACTGGGT GCCATGCTGC TAAATGACAC GCTCCTCCTC 
GAAGGCAACG ATTCCAGTTT GTTCGGCGAG ATGCTTGCCA ACCGGAGTGG CCAACTGGAT 
CTGATCAACG GCACTGGCGG CCTCAATGTG ACCACCAGCA AAGTGGCCGA GGACGACTTT 
ACGCAGCTGC TGCGAATGGC CGTCACATCG GTGCTGCTCG GTCTAATGAT ACTGGTCACC 
ATAATTGGCA ATGTCTTTGT CATAGCGGCC ATTATCCTGG AGCGGAATCT ACAAAACGTG 
GCCAACTACC TGGTGGCCTC ATTGGCAGTG GCCGATCTCT TCGTGGCCTG CCTAGTAATG 
CCCCTAGGAG CTGTATACGA GATCAGCCAA GGCTGGATAC TTGGTCCGGA ATTGTGCGAT 
ATCTGGACGT CGTGCGATGT CCTCTGCTGC ACTGCCTCGA TTTTGCATCT GGTGGCCATT 
GCCGTCGATC GTTACTGGGC GGTCACCAAT ATAGATTACA TCCACTCGCG GACCAGCAAT 
CGTGTCTTTA TGATGATCTT TTGCGTTTGG ACGGCGGCGG TGATTGTGTC CTTGGCACCA 
CAGTTCGGTT GGAAGGATCC AGACTATCTG CAGCGTATCG AGCAGCAAAA GTGCATGGTC 
TCGCAGGATG TTTCTTATCA GGTATTTGCC ACCTGTTGCA CATTTTACGT GCCACTGCTG 
GTCATCCTGG CGTTGTACTG GAAAATCTAT CAAACGGCCC GCAAACGCAT CCATCGTCGA 
CGACCGCGAC CCGTCGATGC GGCGGTCAAT AATAATCAGC CGGACGGAGG TGCGGCAACA 
GATACGAAAC TTCATCGACT GCGTCTACGC CTCGGCAGAT TCTCCACGGC CAAATCCAAA 
ACCGGAAGTG CCGTGGGCGT CTCAGGACCC GCATCCGGAG GCAGGGCTCT GGGCCTCGTC 
GATGGCAACT CAACGAACAC GGTCAACACC GTCGAGGACA CCGAGTTCAG CAGCTCGAAT 
GTGGACAGCA AAAGTCGGGC AGGCGTCGAG GCACCCAGCA CTTCTGGCAA CCAGATAGCC 
ACCGTATCGC ACCTGGTTGC GCTGGCCAAG CAGCAGGGCA AGTCCACCGC CAAATCCTCA 
GCCGCTGTCA ACGGAATGGC CCCATCGGGC AGGCAAGAGG ACGATGGCCA GCGACCAGAA 
CACGGGGAGC AGGAGGATCG CGAGGAGCTA GAGGACCAGG ACGAGCAGGT TGGCCCGCAG 
CCCACAACCG CAACATCAGC AATGACAGCG GCAGGCACCA ACGAGTCGGA GGACCAATGC 
AAGGCCAACG GGGTGGAGGT CCTGGAGGAT CCGCAGTTGC AACAGCAGCT CGAACAAGTG 
CAGCAGCTGC AGAAATCGGT TAAATCCGGC GGTGGAGGTG GGGCCAGTAC GTCAAATGCC 
ACCACAATTA CGTCGATATC AGCGCTATCG CCACAAACGC CAACATCGCA GGGAGTTGGA 
ATCGCCGCCG CCGCCGCTGG ACCGATGACG GCCAAAACCA GCACGCTGAC GAGCTGCAAC 
CAGTCGCATC CGCTATGCGG CACCGCCAAC GAATCCCCAT CGACACCAGA GCCACGTAGC 
CGACAACCGA CGACCCCGCA GCAGCAGCCG CATCAGCAGG CGCACCAGCA GCAGCAGCAG 
CAGCAGCAAC TCTCCAGCAT CGCCAATCCG ATGCAGAAGG TGAACAAGCG AAAGGAGACC 
CTGGAGGCCA AAAGGGAACG GAAGGCGGCC AAGACGCTGG CCATCATTAC CGGCGCCTTC 
GTCGTCTGCT GGCTGCCCTT CTTCGTCATG GCACTGACCA TGCCGCTCTG TGCCGCCTGC 
CAGATCAGCG ATTCCGTTGC CTCGCTCTTC CTTTGGCTGG GCTACTTCAA CTCCACCCTC 
AATCCGGTTA TTTACACCAT CTTCAGTCCG GAGTTCCGAC AGGCCTTCAA GCGCATCCTC 
TTCGGCGGCC ACCGACCAGT CCACTACCGC AGCGGGAAGC TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725849.1
CDS	1057..3561
Translation	MAHETSFNDALDYIYIANSMNDRAFLIAEPHPEQPNVDGQDQDDAELEELDDMAVTDDGQLEDTNNNNNSKRYYSSGKRRADFIGSLALKPPPTDVNTTTTTAGSPLATAALAAAAASASVAAAAARITAKAAHRALTTKQDATSSPASSPALQLIDMDNNYTNVAVGLGAMLLNDTLLLEGNDSSLFGEMLANRSGQLDLINGTGGLNVTTSKVAEDDFTQLLRMAVTSVLLGLMILVTIIGNVFVIAAIILERNLQNVANYLVASLAVADLFVACLVMPLGAVYEISQGWILGPELCDIWTSCDVLCCTASILHLVAIAVDRYWAVTNIDYIHSRTSNRVFMMIFCVWTAAVIVSLAPQFGWKDPDYLQRIEQQKCMVSQDVSYQVFATCCTFYVPLLVILALYWKIYQTARKRIHRRRPRPVDAAVNNNQPDGGAATDTKLHRLRLRLGRFSTAKSKTGSAVGVSGPASGGRALGLVDGNSTNTVNTVEDTEFSSSNVDSKSRAGVEAPSTSGNQIATVSHLVALAKQQGKSTAKSSAAVNGMAPSGRQEDDGQRPEHGEQEDREELEDQDEQVGPQPTTATSAMTAAGTNESEDQCKANGVEVLEDPQLQQQLEQVQQLQKSVKSGGGGGASTSNATTITSISALSPQTPTSQGVGIAAAAAGPMTAKTSTLTSCNQSHPLCGTANESPSTPEPRSRQPTTPQQQPHQQAHQQQQQQQQLSSIANPMQKVNKRKETLEAKRERKAAKTLAIITGAFVVCWLPFFVMALTMPLCAACQISDSVASLFLWLGYFNSTLNPVIYTIFSPEFRQAFKRILFGGHRPVHYRSGKL

Target ORF information:

RefSeq Version	NM_166322.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster 5-hydroxytryptamine (serotonin) receptor 1A (5-HT1A), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166322.2

ORF Insert Sequence:

ATGGCGCACG AGACCAGCTT TAATGATGCG CTTGACTACA TTTACATAGC GAACAGCATG 
AATGACAGGG CCTTTCTGAT AGCCGAACCG CATCCCGAAC AGCCGAATGT AGATGGTCAG 
GACCAGGACG ACGCGGAGCT GGAGGAACTG GACGACATGG CGGTCACGGA CGATGGACAG 
CTGGAGGATA CCAATAACAA CAACAACAGC AAGCGCTACT ACAGCTCCGG TAAAAGACGA 
GCGGATTTCA TAGGCTCCCT GGCGCTGAAG CCACCGCCCA CCGATGTGAA CACCACCACC 
ACCACCGCCG GCTCACCACT GGCCACCGCC GCTTTGGCAG CGGCAGCCGC CAGTGCCTCG 
GTGGCGGCAG CGGCTGCCAG GATCACCGCC AAGGCGGCAC ACAGGGCACT GACCACCAAG 
CAGGATGCCA CATCCTCGCC AGCATCCTCT CCAGCCCTCC AACTGATCGA CATGGACAAT 
AACTACACGA ATGTCGCAGT CGGACTGGGT GCCATGCTGC TAAATGACAC GCTCCTCCTC 
GAAGGCAACG ATTCCAGTTT GTTCGGCGAG ATGCTTGCCA ACCGGAGTGG CCAACTGGAT 
CTGATCAACG GCACTGGCGG CCTCAATGTG ACCACCAGCA AAGTGGCCGA GGACGACTTT 
ACGCAGCTGC TGCGAATGGC CGTCACATCG GTGCTGCTCG GTCTAATGAT ACTGGTCACC 
ATAATTGGCA ATGTCTTTGT CATAGCGGCC ATTATCCTGG AGCGGAATCT ACAAAACGTG 
GCCAACTACC TGGTGGCCTC ATTGGCAGTG GCCGATCTCT TCGTGGCCTG CCTAGTAATG 
CCCCTAGGAG CTGTATACGA GATCAGCCAA GGCTGGATAC TTGGTCCGGA ATTGTGCGAT 
ATCTGGACGT CGTGCGATGT CCTCTGCTGC ACTGCCTCGA TTTTGCATCT GGTGGCCATT 
GCCGTCGATC GTTACTGGGC GGTCACCAAT ATAGATTACA TCCACTCGCG GACCAGCAAT 
CGTGTCTTTA TGATGATCTT TTGCGTTTGG ACGGCGGCGG TGATTGTGTC CTTGGCACCA 
CAGTTCGGTT GGAAGGATCC AGACTATCTG CAGCGTATCG AGCAGCAAAA GTGCATGGTC 
TCGCAGGATG TTTCTTATCA GGTATTTGCC ACCTGTTGCA CATTTTACGT GCCACTGCTG 
GTCATCCTGG CGTTGTACTG GAAAATCTAT CAAACGGCCC GCAAACGCAT CCATCGTCGA 
CGACCGCGAC CCGTCGATGC GGCGGTCAAT AATAATCAGC CGGACGGAGG TGCGGCAACA 
GATACGAAAC TTCATCGACT GCGTCTACGC CTCGGCAGAT TCTCCACGGC CAAATCCAAA 
ACCGGAAGTG CCGTGGGCGT CTCAGGACCC GCATCCGGAG GCAGGGCTCT GGGCCTCGTC 
GATGGCAACT CAACGAACAC GGTCAACACC GTCGAGGACA CCGAGTTCAG CAGCTCGAAT 
GTGGACAGCA AAAGTCGGGC AGGCGTCGAG GCACCCAGCA CTTCTGGCAA CCAGATAGCC 
ACCGTATCGC ACCTGGTTGC GCTGGCCAAG CAGCAGGGCA AGTCCACCGC CAAATCCTCA 
GCCGCTGTCA ACGGAATGGC CCCATCGGGC AGGCAAGAGG ACGATGGCCA GCGACCAGAA 
CACGGGGAGC AGGAGGATCG CGAGGAGCTA GAGGACCAGG ACGAGCAGGT TGGCCCGCAG 
CCCACAACCG CAACATCAGC AATGACAGCG GCAGGCACCA ACGAGTCGGA GGACCAATGC 
AAGGCCAACG GGGTGGAGGT CCTGGAGGAT CCGCAGTTGC AACAGCAGCT CGAACAAGTG 
CAGCAGCTGC AGAAATCGGT TAAATCCGGC GGTGGAGGTG GGGCCAGTAC GTCAAATGCC 
ACCACAATTA CGTCGATATC AGCGCTATCG CCACAAACGC CAACATCGCA GGGAGTTGGA 
ATCGCCGCCG CCGCCGCTGG ACCGATGACG GCCAAAACCA GCACGCTGAC GAGCTGCAAC 
CAGTCGCATC CGCTATGCGG CACCGCCAAC GAATCCCCAT CGACACCAGA GCCACGTAGC 
CGACAACCGA CGACCCCGCA GCAGCAGCCG CATCAGCAGG CGCACCAGCA GCAGCAGCAG 
CAGCAGCAAC TCTCCAGCAT CGCCAATCCG ATGCAGAAGG TGAACAAGCG AAAGGAGACC 
CTGGAGGCCA AAAGGGAACG GAAGGCGGCC AAGACGCTGG CCATCATTAC CGGCGCCTTC 
GTCGTCTGCT GGCTGCCCTT CTTCGTCATG GCACTGACCA TGCCGCTCTG TGCCGCCTGC 
CAGATCAGCG ATTCCGTTGC CTCGCTCTTC CTTTGGCTGG GCTACTTCAA CTCCACCCTC 
AATCCGGTTA TTTACACCAT CTTCAGTCCG GAGTTCCGAC AGGCCTTCAA GCGCATCCTC 
TTCGGCGGCC ACCGACCAGT CCACTACCGC AGCGGGAAGC TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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5-HT1A cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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