GEFmeso cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following GEFmeso gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GEFmeso cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa12188 NM_001274146.2
Latest version!
Drosophila melanogaster guanine nucleotide exchange factor in mesoderm, transcript variant F (GEFmeso), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa21852 NM_137499.3
Latest version!
Drosophila melanogaster guanine nucleotide exchange factor in mesoderm, transcript variant G (GEFmeso), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OFa10503 NM_176223.4
Latest version!
Drosophila melanogaster guanine nucleotide exchange factor in mesoderm, transcript variant E (GEFmeso), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa12188
    Clone ID Related Accession (Same CDS sequence) NM_001274146.2
    Accession Version NM_001274146.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2637bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product guanine nucleotide exchange factor in mesoderm, isoform F
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001274146.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGACATAG AATCCGATTC GGACACCTCG GAGAGATGGG AGGACTTTTT TGGCAGCTCC 
    ACCGAATTGG CACCCTCGCT GCTGGGCATC TACGACACAT TGACAAACAG CTTTGCCGCG
    GGGGGCGTGG CAGCTGCTCC CACTGCGACA TCTCACGAAT CGAAGGCATC CAGCAGCGGC
    GACTCGACGC CCAAGAGGAG CAACAGTAAC TGCAGCAGCA GCAGCAGCAC TGGCTCATCG
    ACTGGCAGCT GTTCGAGTGT CCCCACCAGC GATGACCAGT CTGCTAGCTA CACGCTAGCC
    AGCTCCAGCA CTCATTTGGG CCACCAGGAG CCGCCCGCTC CACTGGTGGT TGCGGGCCTG
    CGGGACGAAC CGGATGGCGC TCAGTTGAGC AGGTTGGTGC AGCGCAGAAG CCAGGAGTCG
    CAGATTCACG GCAGCAGCAG CAGCATAGCC TCGAGTGGGA GCAGTCGACG GGGCAGCAAC
    ATCCGCATCC TTTCGCCCAA TGTTCATAGA ATCATCACGC ACTCGGATAT TCAACCAGTG
    GAGGCGGTGT CCGCGGAGGC ACTGAAACAG CATCAGCAGC AATCCCAGAG GAGTCCCACC
    CAGGGCAGGT ATATAAAGAC CGGTCATAGC GGAACACCAC CAGCCGCTGG TTCCGCACCC
    AAGCTGAAGC TCTCGCACAT ACCGCTGTCC AAGATCACCG GCAAGTTGTC CACCCAGTCT
    GGCAGCGAAC TGGTGTCGAC CTTCGATTCC AGCAACGAGT ATTTGAACTC GGTGGCCTTC
    GCCGCTTTCG AGAAATCCAA GCCGCAGGAC GAGGATCGGA CGCCAACGAA CAAATCGGTA
    CCAGGCACTC CCTTCCATTT CGATGGGCAT CCGTTTCAGG GGCGAAAACT AACCCTACCA
    CGGTACGATT CACAGCAGAG CATCAAGCTG ATCGAAATGG AGCAGCTGGC GGCCACCAGT
    GCGCAACTGT TGCATGCCAA GCCTGCCACG CCCGGAACTC CGGCCACGCC CACTCCCAGT
    GCAACCAAAG CCAAGGAGTT TGTGTGCAGT GAGCCACTGA GTCCCGTGGA TATAGTGGAC
    ACCATTAACT TCGATCTGGT TGCGCAGCAT ATGGAAAGAT TGACTCTGGC GGATCCAGGA
    GATTTGGATC CCAGGGCGGA GCTAATAGAA GCGGTTAACA AAACACTGGT TAACAAGCCA
    CTGGTTAGGA GCTCCTCCGC CGCCTGTGCC TCCACAATAT GCAGTTCACC GCTGTTGACC
    TATGCCCGCA CCAAGAGTTT GGGCGCCAAG GCCAGCACTT TGGGCGGTGG AGTGCCTATT
    AAGCTGCCCA CTGCCGAACA GCTGGCCGAG CTGGAGGAGG CCAACAAGTT GTCGGCGGAA
    AGTCTGGACA GGCTCACCGA TATCAAGCCC AAGTTTGCAG CTCGCCTAAG TGAGCTGGCT
    AGGCTGAATA ACCGCCCACT GTCCTCCTCC TCCATTTGCT CCACCTCCTC CAGTTCCAGT
    TCCGGTTCGG ACCAACTGCT CAATGGAAAA CTGATGGCCA CTTCCTACTT GGCTAGCGTA
    GAAAGTCTGG CGGAGAGTGA GAACGAGCTG GGCGATCAAC ACCATCCGCC GGCGATGAGT
    GTCCTCGAGA AAACCTGCCT AGAGATCGTA GACTCGGAGC GAAGCTTCGT CGAGGATTTG
    GGTCAGGTCA TCAAGGGATA CCTGCAGGAC TGGAAGGAGC GTGCGTGCCT GCGCGTAGAC
    GAATTGCAAA TACTCTTCGC CAACATTGAA GAGATTTATG AATTCAACTC AATGCTTCTG
    CAGCGACTTA TCAACACGGG AAGGGATCCC GGGAGGATCG CCAGGTGCTT CATTGACCTG
    CGCGATGGCT TTGATGTGTA CACAACCTAC TGCACAAGCT ACCCGGAAGC CATATCGTTG
    CTGACCAAGT TGCTGCAGGC CACGCACACG TACTCTCTGC TTGCCTCCAC GCAGAAGCTA
    CTCCAACATC GCCTGCCCCT GGGATCCTAC CTCCTGAAAC CGGTGCAGCG CATCCTCAAG
    TACCATCTGC TGCTGGACAG CCTGCGCAAG CACTGCGATG TGAAGGAGGT GGTCGAGGCC
    CATGTGATCA TGCGCCAGGT GGCGCACAAC ATTGACCAGG TGAAGCGAAA GCAGGAGCAG
    CAGAGTCGCG TCAAGGAGCT GTCCGGCATT CTGGACGGCT GGCTGGGGCC AGAGCTCACT
    GTTCTGGGAG AGTTGCGCCA AGAGGGCCTG CTGATGGAGC AGCACAATAA ACAGCGCCTG
    GTACTGCTAT TCGCCACCAT GCTGATCATC ACCAAGCAAA AGGAGGACGG ACGCCTTCAG
    TTCAAGACTT ACATTTCGCA AAACAATCTG ATGCTGAGCG AACACTTGCC CGGTGAACCG
    ACCAGCTTCT ATGTCATCCC CTTCGACGAG CCGCGCCATC AAATCAAACT GACGGCTCGG
    AATCGTGACC AGAAGCGCAT CTGGACGCAG CACATCAAAG GTGTCATGCT GGAGAAGTTA
    GACATTCCGA TGCGCGCCAA GGAGCTGGTC TATCAGCTGG GCAACGAGGA AGTACTACCA
    GTGATGCATT CTAAAACTAA ACACCCCAAA ACAAAGAAAC GAAAAAACTT TGACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001261075.1
    CDS1046..3682
    Translation

    Target ORF information:

    RefSeq Version NM_001274146.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster guanine nucleotide exchange factor in mesoderm, transcript variant F (GEFmeso), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001274146.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGACATAG AATCCGATTC GGACACCTCG GAGAGATGGG AGGACTTTTT TGGCAGCTCC 
    ACCGAATTGG CACCCTCGCT GCTGGGCATC TACGACACAT TGACAAACAG CTTTGCCGCG
    GGGGGCGTGG CAGCTGCTCC CACTGCGACA TCTCACGAAT CGAAGGCATC CAGCAGCGGC
    GACTCGACGC CCAAGAGGAG CAACAGTAAC TGCAGCAGCA GCAGCAGCAC TGGCTCATCG
    ACTGGCAGCT GTTCGAGTGT CCCCACCAGC GATGACCAGT CTGCTAGCTA CACGCTAGCC
    AGCTCCAGCA CTCATTTGGG CCACCAGGAG CCGCCCGCTC CACTGGTGGT TGCGGGCCTG
    CGGGACGAAC CGGATGGCGC TCAGTTGAGC AGGTTGGTGC AGCGCAGAAG CCAGGAGTCG
    CAGATTCACG GCAGCAGCAG CAGCATAGCC TCGAGTGGGA GCAGTCGACG GGGCAGCAAC
    ATCCGCATCC TTTCGCCCAA TGTTCATAGA ATCATCACGC ACTCGGATAT TCAACCAGTG
    GAGGCGGTGT CCGCGGAGGC ACTGAAACAG CATCAGCAGC AATCCCAGAG GAGTCCCACC
    CAGGGCAGGT ATATAAAGAC CGGTCATAGC GGAACACCAC CAGCCGCTGG TTCCGCACCC
    AAGCTGAAGC TCTCGCACAT ACCGCTGTCC AAGATCACCG GCAAGTTGTC CACCCAGTCT
    GGCAGCGAAC TGGTGTCGAC CTTCGATTCC AGCAACGAGT ATTTGAACTC GGTGGCCTTC
    GCCGCTTTCG AGAAATCCAA GCCGCAGGAC GAGGATCGGA CGCCAACGAA CAAATCGGTA
    CCAGGCACTC CCTTCCATTT CGATGGGCAT CCGTTTCAGG GGCGAAAACT AACCCTACCA
    CGGTACGATT CACAGCAGAG CATCAAGCTG ATCGAAATGG AGCAGCTGGC GGCCACCAGT
    GCGCAACTGT TGCATGCCAA GCCTGCCACG CCCGGAACTC CGGCCACGCC CACTCCCAGT
    GCAACCAAAG CCAAGGAGTT TGTGTGCAGT GAGCCACTGA GTCCCGTGGA TATAGTGGAC
    ACCATTAACT TCGATCTGGT TGCGCAGCAT ATGGAAAGAT TGACTCTGGC GGATCCAGGA
    GATTTGGATC CCAGGGCGGA GCTAATAGAA GCGGTTAACA AAACACTGGT TAACAAGCCA
    CTGGTTAGGA GCTCCTCCGC CGCCTGTGCC TCCACAATAT GCAGTTCACC GCTGTTGACC
    TATGCCCGCA CCAAGAGTTT GGGCGCCAAG GCCAGCACTT TGGGCGGTGG AGTGCCTATT
    AAGCTGCCCA CTGCCGAACA GCTGGCCGAG CTGGAGGAGG CCAACAAGTT GTCGGCGGAA
    AGTCTGGACA GGCTCACCGA TATCAAGCCC AAGTTTGCAG CTCGCCTAAG TGAGCTGGCT
    AGGCTGAATA ACCGCCCACT GTCCTCCTCC TCCATTTGCT CCACCTCCTC CAGTTCCAGT
    TCCGGTTCGG ACCAACTGCT CAATGGAAAA CTGATGGCCA CTTCCTACTT GGCTAGCGTA
    GAAAGTCTGG CGGAGAGTGA GAACGAGCTG GGCGATCAAC ACCATCCGCC GGCGATGAGT
    GTCCTCGAGA AAACCTGCCT AGAGATCGTA GACTCGGAGC GAAGCTTCGT CGAGGATTTG
    GGTCAGGTCA TCAAGGGATA CCTGCAGGAC TGGAAGGAGC GTGCGTGCCT GCGCGTAGAC
    GAATTGCAAA TACTCTTCGC CAACATTGAA GAGATTTATG AATTCAACTC AATGCTTCTG
    CAGCGACTTA TCAACACGGG AAGGGATCCC GGGAGGATCG CCAGGTGCTT CATTGACCTG
    CGCGATGGCT TTGATGTGTA CACAACCTAC TGCACAAGCT ACCCGGAAGC CATATCGTTG
    CTGACCAAGT TGCTGCAGGC CACGCACACG TACTCTCTGC TTGCCTCCAC GCAGAAGCTA
    CTCCAACATC GCCTGCCCCT GGGATCCTAC CTCCTGAAAC CGGTGCAGCG CATCCTCAAG
    TACCATCTGC TGCTGGACAG CCTGCGCAAG CACTGCGATG TGAAGGAGGT GGTCGAGGCC
    CATGTGATCA TGCGCCAGGT GGCGCACAAC ATTGACCAGG TGAAGCGAAA GCAGGAGCAG
    CAGAGTCGCG TCAAGGAGCT GTCCGGCATT CTGGACGGCT GGCTGGGGCC AGAGCTCACT
    GTTCTGGGAG AGTTGCGCCA AGAGGGCCTG CTGATGGAGC AGCACAATAA ACAGCGCCTG
    GTACTGCTAT TCGCCACCAT GCTGATCATC ACCAAGCAAA AGGAGGACGG ACGCCTTCAG
    TTCAAGACTT ACATTTCGCA AAACAATCTG ATGCTGAGCG AACACTTGCC CGGTGAACCG
    ACCAGCTTCT ATGTCATCCC CTTCGACGAG CCGCGCCATC AAATCAAACT GACGGCTCGG
    AATCGTGACC AGAAGCGCAT CTGGACGCAG CACATCAAAG GTGTCATGCT GGAGAAGTTA
    GACATTCCGA TGCGCGCCAA GGAGCTGGTC TATCAGCTGG GCAACGAGGA AGTACTACCA
    GTGATGCATT CTAAAACTAA ACACCCCAAA ACAAAGAAAC GAAAAAACTT TGACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa21852
    Clone ID Related Accession (Same CDS sequence) NM_137499.3
    Accession Version NM_137499.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3714bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product guanine nucleotide exchange factor in mesoderm, isoform G
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137499.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGAGCAGC TGGCGGCCAC CAGTGCGCAA CTGTTGCATG CCAAGCCTGC CACGCCCGGA 
    ACTCCGGCCA CGCCCACTCC CAGTGCAACC AAAGCCAAGG AGTTTGTGTG CAGTGAGCCA
    CTGAGTCCCG TGGATATAGT GGACACCATT AACTTCGATC TGGTTGCGCA GCATATGGAA
    AGATTGACTC TGGCGGATCC AGGAGATTTG GATCCCAGGG CGGAGCTAAT AGAAGCGGTT
    AACAAAACAC TGGTTAACAA GCCACTGGTT AGGAGCTCCT CCGCCGCCTG TGCCTCCACA
    ATATGCAGTT CACCGCTGTT GACCTATGCC CGCACCAAGA GTTTGGGCGC CAAGGCCAGC
    ACTTTGGGCG GTGGAGTGCC TATTAAGCTG CCCACTGCCG AACAGCTGGC CGAGCTGGAG
    GAGGCCAACA AGTTGTCGGC GGAAAGTCTG GACAGGCTCA CCGATATCAA GCCCAAGTTT
    GCAGCTCGCC TAAGTGAGCT GGCTAGGCTG AATAACCGCC CACTGTCCTC CTCCTCCATT
    TGCTCCACCT CCTCCAGTTC CAGTTCCGGT TCGGACCAAC TGCTCAATGG AAAACTGATG
    GCCACTTCCT ACTTGGCTAG CGTAGAAAGT CTGGCGGAGA GTGAGAACGA GCTGGGCGAT
    CAACACCATC CGCCGGCGAT GAGTGTCCTC GAGAAAACCT GCCTAGAGAT CGTAGACTCG
    GAGCGAAGCT TCGTCGAGGA TTTGGGTCAG GTCATCAAGG GATACCTGCA GGACTGGAAG
    GAGCGTGCGT GCCTGCGCGT AGACGAATTG CAAATACTCT TCGCCAACAT TGAAGAGATT
    TATGAATTCA ACTCAATGCT TCTGCAGCGA CTTATCAACA CGGGAAGGGA TCCCGGGAGG
    ATCGCCAGGT GCTTCATTGA CCTGCGCGAT GGCTTTGATG TGTACACAAC CTACTGCACA
    AGCTACCCGG AAGCCATATC GTTGCTGACC AAGTTGCTGC AGGCCACGCA CACGTACTCT
    CTGCTTGCCT CCACGCAGAA GCTACTCCAA CATCGCCTGC CCCTGGGATC CTACCTCCTG
    AAACCGGTGC AGCGCATCCT CAAGTACCAT CTGCTGCTGG ACAGCCTGCG CAAGCACTGC
    GATGTGAAGG AGGTGGTCGA GGCCCATGTG ATCATGCGCC AGGTGGCGCA CAACATTGAC
    CAGGTGAAGC GAAAGCAGGA GCAGCAGAGT CGCGTCAAGG AGCTGTCCGG CATTCTGGAC
    GGCTGGCTGG GGCCAGAGCT CACTGTTCTG GGAGAGTTGC GCCAAGAGGG CCTGCTGATG
    GAGCAGCACA ATAAACAGCG CCTGGTACTG CTATTCGCCA CCATGCTGAT CATCACCAAG
    CAAAAGGAGG ACGGACGCCT TCAGTTCAAG ACTTACATTT CGCAAAACAA TCTGATGCTG
    AGCGAACACT TGCCCGGTGA ACCGACCAGC TTCTATGTCA TCCCCTTCGA CGAGCCGCGC
    CATCAAATCA AACTGACGGC TCGGAATCGT GACCAGAAGC GCATCTGGAC GCAGCACATC
    AAAGGTGTCA TGCTGGAGAA GTTAGACATT CCGATGCGCG CCAAGGAGCT GGTCTATCAG
    CTGGGCAACG AGGAAGATCG CACGCCAGAT CGCAGCACAT GGAAGTGGAG CCTTCACTCG
    GGAAGTAACT CGACTCCAAC TTACCTGGAG CGAAGGAACG CCTGCCGGAG GAGTGAGATA
    CGCCAGCGGA ATTCAAAGTT CAAGCGGAAG ACGGTGGCCA ACTCCAGTTC CTTTGACAGT
    TTCAATGAGT CCTTGGAACA GGAGGAGGAA TCGATTAGCC AGGCGAAGCC CTCGAAGCCA
    CTGGCCAGAA ATAACAGCCT AGACGATACT GCTCTACAGA AACTTGCTGC TGCTATGAGA
    TATCGCAAAA GAGATGCTGC AGTGAAGGAG GAGACGAAGA CGCCGGTGAA GGAGCCTTGT
    AAGTGCGACC AGGATGCGAA ACAGGACGAG CGGATTGAAC CGTGTTCCTG CATTCTGCGG
    GATCAGCGCA GTCGCAGTGC CAAGTCACAA TCTCCTGTCT TCAGCTACAA GGCACTCAAG
    GAGCGCAGCA AGTCGGTTCC CCGGATCGGA GGCTTTAGTT TGGACGAGGA GGCTGAGAGA
    GAACGGGAAC AGGACGAGGA TAGCGCAGCC TCTCTGAGGG GCAGCAAGCC CGATCTTAGC
    GAGCGCAAGA CCAAGGGAAA GGGATTCTTT GAAGTTAAGC AATACAACCA CAAGACGATG
    CCCAAAAAGA TAGCCAATTT GAAGAAGCAA CGAAGTAGCA CCACCAAGAG CTGCTCCAGG
    TTCTACATGG ATCTCAGCGA ATTCGATAGC AGCAGTTCGA CTGTACTCAA GATAACCGAA
    TCCACCGAGG AGCTGCGTCC AGATGGGGAA ATCGAACTGG CCCAAGAAGC CGCCACTCTG
    GATGAGTCCA CGCAGGATCA GTATTACAGC CCAGATCACG AGACGGAACT ACTGTCCCCA
    GCAGAGAAGT CAAAGCGAGA TGCACAGATT GTCCTTGATT TGCTAAAGAA TAGCAAGGAA
    TTTGAGAGGA TCTACAACAA GCAACAGAAG AGGCGCGAAA GTCCAGTGAG TCCCGTGAGT
    CCCGATATAA GTCGTGGTCC CATTCTGCCG CCTCCGCGTC CACCATCGAG ATCTCCACCA
    CCCTTGGAAC TGGAGGAGCA GGAGAAGAAA CCAGTCGAAG AGGTGGATTC AGTCAGCGAG
    GAGCCCATTT ACGAGACTCT GCTGCGCAAT GTCCATGTGC CCTACAAATT CTCACCGGTG
    TTGGGCCGAG CCAAGTCCTC GCAGATACTG AAGAAGCGAT CAAAGACTGC TCCCGCAGCC
    TCACGACCGG AATCGGATTA CGTGACCCTA GTCTACTCGC CCGAAGGAGT TCTGCAGCGA
    GTGGGCGAGG AGGTGGTGCA GAGGGATAGC TGCAGTTCCT CCACCACTTC GACATCCTCA
    AATGGATCGG GGTCCACGCT GAAACGAGAC AGCCAGAGCA CAGTGATCAA TCTTTTGATG
    GAGGCGGCGC AAGAGCACGA ATCACGTCCC TTGCCGAAGA ATTCAGTTTC AGGATCGGAG
    AGTTCTCTGC TGCCACGGGC TCTGAAGTCC ATAGACAATC TGAGCATGGC CTTCGGACGC
    TCCAACCGAC CGCCGGAGCG CCGGGTGTCC GATGTGAGCG AGATGTGCCG TCAGAGTGTG
    CTTCATCGCC AGGGCAGCGA GGCGGTGGGT GAGAGAATGG CCCATGTGGA TTACGCTGAT
    CCCAAGGCTC TATTTGGACT GGGGGTCCTG AACCACTCGG AACGTGACTC GGTCTTCTCG
    CTGACCTCCT CCTCCAGTGA CAGCATGTGC GAGCAGCAGC GCAAGCGATC CGGCGTGGGC
    GCCACCTTCG AGTATGAGCA GCAGGTGGAG GATAGCTTGG AGAATGATTT TCGCGACTCG
    GCCATTTACA GCGATGACAA CGAGAAGCGT TCCGGGGACT ACGATGTCCA TCTACGCCGT
    CCCAGCAGTC CGCCACCACC ACCGCCTCCG CTGGGGCCGC GTCCCCATCA GCCACCACAG
    ATTGCGCCGA AGCCTTCCAA GGATCAGCTA ATCCAGCAGC GATCGGGCGT CATCGTCTGC
    CAGTCGGCCT CCCGCAGCTG GGTGCTTCAG CAGATTGAGA ACTTCAACAA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_611343.4
    CDS1673..5386
    Translation

    Target ORF information:

    RefSeq Version NM_137499.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster guanine nucleotide exchange factor in mesoderm, transcript variant G (GEFmeso), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_137499.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGAGCAGC TGGCGGCCAC CAGTGCGCAA CTGTTGCATG CCAAGCCTGC CACGCCCGGA 
    ACTCCGGCCA CGCCCACTCC CAGTGCAACC AAAGCCAAGG AGTTTGTGTG CAGTGAGCCA
    CTGAGTCCCG TGGATATAGT GGACACCATT AACTTCGATC TGGTTGCGCA GCATATGGAA
    AGATTGACTC TGGCGGATCC AGGAGATTTG GATCCCAGGG CGGAGCTAAT AGAAGCGGTT
    AACAAAACAC TGGTTAACAA GCCACTGGTT AGGAGCTCCT CCGCCGCCTG TGCCTCCACA
    ATATGCAGTT CACCGCTGTT GACCTATGCC CGCACCAAGA GTTTGGGCGC CAAGGCCAGC
    ACTTTGGGCG GTGGAGTGCC TATTAAGCTG CCCACTGCCG AACAGCTGGC CGAGCTGGAG
    GAGGCCAACA AGTTGTCGGC GGAAAGTCTG GACAGGCTCA CCGATATCAA GCCCAAGTTT
    GCAGCTCGCC TAAGTGAGCT GGCTAGGCTG AATAACCGCC CACTGTCCTC CTCCTCCATT
    TGCTCCACCT CCTCCAGTTC CAGTTCCGGT TCGGACCAAC TGCTCAATGG AAAACTGATG
    GCCACTTCCT ACTTGGCTAG CGTAGAAAGT CTGGCGGAGA GTGAGAACGA GCTGGGCGAT
    CAACACCATC CGCCGGCGAT GAGTGTCCTC GAGAAAACCT GCCTAGAGAT CGTAGACTCG
    GAGCGAAGCT TCGTCGAGGA TTTGGGTCAG GTCATCAAGG GATACCTGCA GGACTGGAAG
    GAGCGTGCGT GCCTGCGCGT AGACGAATTG CAAATACTCT TCGCCAACAT TGAAGAGATT
    TATGAATTCA ACTCAATGCT TCTGCAGCGA CTTATCAACA CGGGAAGGGA TCCCGGGAGG
    ATCGCCAGGT GCTTCATTGA CCTGCGCGAT GGCTTTGATG TGTACACAAC CTACTGCACA
    AGCTACCCGG AAGCCATATC GTTGCTGACC AAGTTGCTGC AGGCCACGCA CACGTACTCT
    CTGCTTGCCT CCACGCAGAA GCTACTCCAA CATCGCCTGC CCCTGGGATC CTACCTCCTG
    AAACCGGTGC AGCGCATCCT CAAGTACCAT CTGCTGCTGG ACAGCCTGCG CAAGCACTGC
    GATGTGAAGG AGGTGGTCGA GGCCCATGTG ATCATGCGCC AGGTGGCGCA CAACATTGAC
    CAGGTGAAGC GAAAGCAGGA GCAGCAGAGT CGCGTCAAGG AGCTGTCCGG CATTCTGGAC
    GGCTGGCTGG GGCCAGAGCT CACTGTTCTG GGAGAGTTGC GCCAAGAGGG CCTGCTGATG
    GAGCAGCACA ATAAACAGCG CCTGGTACTG CTATTCGCCA CCATGCTGAT CATCACCAAG
    CAAAAGGAGG ACGGACGCCT TCAGTTCAAG ACTTACATTT CGCAAAACAA TCTGATGCTG
    AGCGAACACT TGCCCGGTGA ACCGACCAGC TTCTATGTCA TCCCCTTCGA CGAGCCGCGC
    CATCAAATCA AACTGACGGC TCGGAATCGT GACCAGAAGC GCATCTGGAC GCAGCACATC
    AAAGGTGTCA TGCTGGAGAA GTTAGACATT CCGATGCGCG CCAAGGAGCT GGTCTATCAG
    CTGGGCAACG AGGAAGATCG CACGCCAGAT CGCAGCACAT GGAAGTGGAG CCTTCACTCG
    GGAAGTAACT CGACTCCAAC TTACCTGGAG CGAAGGAACG CCTGCCGGAG GAGTGAGATA
    CGCCAGCGGA ATTCAAAGTT CAAGCGGAAG ACGGTGGCCA ACTCCAGTTC CTTTGACAGT
    TTCAATGAGT CCTTGGAACA GGAGGAGGAA TCGATTAGCC AGGCGAAGCC CTCGAAGCCA
    CTGGCCAGAA ATAACAGCCT AGACGATACT GCTCTACAGA AACTTGCTGC TGCTATGAGA
    TATCGCAAAA GAGATGCTGC AGTGAAGGAG GAGACGAAGA CGCCGGTGAA GGAGCCTTGT
    AAGTGCGACC AGGATGCGAA ACAGGACGAG CGGATTGAAC CGTGTTCCTG CATTCTGCGG
    GATCAGCGCA GTCGCAGTGC CAAGTCACAA TCTCCTGTCT TCAGCTACAA GGCACTCAAG
    GAGCGCAGCA AGTCGGTTCC CCGGATCGGA GGCTTTAGTT TGGACGAGGA GGCTGAGAGA
    GAACGGGAAC AGGACGAGGA TAGCGCAGCC TCTCTGAGGG GCAGCAAGCC CGATCTTAGC
    GAGCGCAAGA CCAAGGGAAA GGGATTCTTT GAAGTTAAGC AATACAACCA CAAGACGATG
    CCCAAAAAGA TAGCCAATTT GAAGAAGCAA CGAAGTAGCA CCACCAAGAG CTGCTCCAGG
    TTCTACATGG ATCTCAGCGA ATTCGATAGC AGCAGTTCGA CTGTACTCAA GATAACCGAA
    TCCACCGAGG AGCTGCGTCC AGATGGGGAA ATCGAACTGG CCCAAGAAGC CGCCACTCTG
    GATGAGTCCA CGCAGGATCA GTATTACAGC CCAGATCACG AGACGGAACT ACTGTCCCCA
    GCAGAGAAGT CAAAGCGAGA TGCACAGATT GTCCTTGATT TGCTAAAGAA TAGCAAGGAA
    TTTGAGAGGA TCTACAACAA GCAACAGAAG AGGCGCGAAA GTCCAGTGAG TCCCGTGAGT
    CCCGATATAA GTCGTGGTCC CATTCTGCCG CCTCCGCGTC CACCATCGAG ATCTCCACCA
    CCCTTGGAAC TGGAGGAGCA GGAGAAGAAA CCAGTCGAAG AGGTGGATTC AGTCAGCGAG
    GAGCCCATTT ACGAGACTCT GCTGCGCAAT GTCCATGTGC CCTACAAATT CTCACCGGTG
    TTGGGCCGAG CCAAGTCCTC GCAGATACTG AAGAAGCGAT CAAAGACTGC TCCCGCAGCC
    TCACGACCGG AATCGGATTA CGTGACCCTA GTCTACTCGC CCGAAGGAGT TCTGCAGCGA
    GTGGGCGAGG AGGTGGTGCA GAGGGATAGC TGCAGTTCCT CCACCACTTC GACATCCTCA
    AATGGATCGG GGTCCACGCT GAAACGAGAC AGCCAGAGCA CAGTGATCAA TCTTTTGATG
    GAGGCGGCGC AAGAGCACGA ATCACGTCCC TTGCCGAAGA ATTCAGTTTC AGGATCGGAG
    AGTTCTCTGC TGCCACGGGC TCTGAAGTCC ATAGACAATC TGAGCATGGC CTTCGGACGC
    TCCAACCGAC CGCCGGAGCG CCGGGTGTCC GATGTGAGCG AGATGTGCCG TCAGAGTGTG
    CTTCATCGCC AGGGCAGCGA GGCGGTGGGT GAGAGAATGG CCCATGTGGA TTACGCTGAT
    CCCAAGGCTC TATTTGGACT GGGGGTCCTG AACCACTCGG AACGTGACTC GGTCTTCTCG
    CTGACCTCCT CCTCCAGTGA CAGCATGTGC GAGCAGCAGC GCAAGCGATC CGGCGTGGGC
    GCCACCTTCG AGTATGAGCA GCAGGTGGAG GATAGCTTGG AGAATGATTT TCGCGACTCG
    GCCATTTACA GCGATGACAA CGAGAAGCGT TCCGGGGACT ACGATGTCCA TCTACGCCGT
    CCCAGCAGTC CGCCACCACC ACCGCCTCCG CTGGGGCCGC GTCCCCATCA GCCACCACAG
    ATTGCGCCGA AGCCTTCCAA GGATCAGCTA ATCCAGCAGC GATCGGGCGT CATCGTCTGC
    CAGTCGGCCT CCCGCAGCTG GGTGCTTCAG CAGATTGAGA ACTTCAACAA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa10503
    Clone ID Related Accession (Same CDS sequence) NM_176223.4
    Accession Version NM_176223.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4650bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product guanine nucleotide exchange factor in mesoderm, isoform E
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_176223.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGACATAG AATCCGATTC GGACACCTCG GAGAGATGGG AGGACTTTTT TGGCAGCTCC 
    ACCGAATTGG CACCCTCGCT GCTGGGCATC TACGACACAT TGACAAACAG CTTTGCCGCG
    GGGGGCGTGG CAGCTGCTCC CACTGCGACA TCTCACGAAT CGAAGGCATC CAGCAGCGGC
    GACTCGACGC CCAAGAGGAG CAACAGTAAC TGCAGCAGCA GCAGCAGCAC TGGCTCATCG
    ACTGGCAGCT GTTCGAGTGT CCCCACCAGC GATGACCAGT CTGCTAGCTA CACGCTAGCC
    AGCTCCAGCA CTCATTTGGG CCACCAGGAG CCGCCCGCTC CACTGGTGGT TGCGGGCCTG
    CGGGACGAAC CGGATGGCGC TCAGTTGAGC AGGTTGGTGC AGCGCAGAAG CCAGGAGTCG
    CAGATTCACG GCAGCAGCAG CAGCATAGCC TCGAGTGGGA GCAGTCGACG GGGCAGCAAC
    ATCCGCATCC TTTCGCCCAA TGTTCATAGA ATCATCACGC ACTCGGATAT TCAACCAGTG
    GAGGCGGTGT CCGCGGAGGC ACTGAAACAG CATCAGCAGC AATCCCAGAG GAGTCCCACC
    CAGGGCAGGT ATATAAAGAC CGGTCATAGC GGAACACCAC CAGCCGCTGG TTCCGCACCC
    AAGCTGAAGC TCTCGCACAT ACCGCTGTCC AAGATCACCG GCAAGTTGTC CACCCAGTCT
    GGCAGCGAAC TGGTGTCGAC CTTCGATTCC AGCAACGAGT ATTTGAACTC GGTGGCCTTC
    GCCGCTTTCG AGAAATCCAA GCCGCAGGAC GAGGATCGGA CGCCAACGAA CAAATCGGTA
    CCAGGCACTC CCTTCCATTT CGATGGGCAT CCGTTTCAGG GGCGAAAACT AACCCTACCA
    CGGTACGATT CACAGCAGAG CATCAAGCTG ATCGAAATGG AGCAGCTGGC GGCCACCAGT
    GCGCAACTGT TGCATGCCAA GCCTGCCACG CCCGGAACTC CGGCCACGCC CACTCCCAGT
    GCAACCAAAG CCAAGGAGTT TGTGTGCAGT GAGCCACTGA GTCCCGTGGA TATAGTGGAC
    ACCATTAACT TCGATCTGGT TGCGCAGCAT ATGGAAAGAT TGACTCTGGC GGATCCAGGA
    GATTTGGATC CCAGGGCGGA GCTAATAGAA GCGGTTAACA AAACACTGGT TAACAAGCCA
    CTGGTTAGGA GCTCCTCCGC CGCCTGTGCC TCCACAATAT GCAGTTCACC GCTGTTGACC
    TATGCCCGCA CCAAGAGTTT GGGCGCCAAG GCCAGCACTT TGGGCGGTGG AGTGCCTATT
    AAGCTGCCCA CTGCCGAACA GCTGGCCGAG CTGGAGGAGG CCAACAAGTT GTCGGCGGAA
    AGTCTGGACA GGCTCACCGA TATCAAGCCC AAGTTTGCAG CTCGCCTAAG TGAGCTGGCT
    AGGCTGAATA ACCGCCCACT GTCCTCCTCC TCCATTTGCT CCACCTCCTC CAGTTCCAGT
    TCCGGTTCGG ACCAACTGCT CAATGGAAAA CTGATGGCCA CTTCCTACTT GGCTAGCGTA
    GAAAGTCTGG CGGAGAGTGA GAACGAGCTG GGCGATCAAC ACCATCCGCC GGCGATGAGT
    GTCCTCGAGA AAACCTGCCT AGAGATCGTA GACTCGGAGC GAAGCTTCGT CGAGGATTTG
    GGTCAGGTCA TCAAGGGATA CCTGCAGGAC TGGAAGGAGC GTGCGTGCCT GCGCGTAGAC
    GAATTGCAAA TACTCTTCGC CAACATTGAA GAGATTTATG AATTCAACTC AATGCTTCTG
    CAGCGACTTA TCAACACGGG AAGGGATCCC GGGAGGATCG CCAGGTGCTT CATTGACCTG
    CGCGATGGCT TTGATGTGTA CACAACCTAC TGCACAAGCT ACCCGGAAGC CATATCGTTG
    CTGACCAAGT TGCTGCAGGC CACGCACACG TACTCTCTGC TTGCCTCCAC GCAGAAGCTA
    CTCCAACATC GCCTGCCCCT GGGATCCTAC CTCCTGAAAC CGGTGCAGCG CATCCTCAAG
    TACCATCTGC TGCTGGACAG CCTGCGCAAG CACTGCGATG TGAAGGAGGT GGTCGAGGCC
    CATGTGATCA TGCGCCAGGT GGCGCACAAC ATTGACCAGG TGAAGCGAAA GCAGGAGCAG
    CAGAGTCGCG TCAAGGAGCT GTCCGGCATT CTGGACGGCT GGCTGGGGCC AGAGCTCACT
    GTTCTGGGAG AGTTGCGCCA AGAGGGCCTG CTGATGGAGC AGCACAATAA ACAGCGCCTG
    GTACTGCTAT TCGCCACCAT GCTGATCATC ACCAAGCAAA AGGAGGACGG ACGCCTTCAG
    TTCAAGACTT ACATTTCGCA AAACAATCTG ATGCTGAGCG AACACTTGCC CGGTGAACCG
    ACCAGCTTCT ATGTCATCCC CTTCGACGAG CCGCGCCATC AAATCAAACT GACGGCTCGG
    AATCGTGACC AGAAGCGCAT CTGGACGCAG CACATCAAAG GTGTCATGCT GGAGAAGTTA
    GACATTCCGA TGCGCGCCAA GGAGCTGGTC TATCAGCTGG GCAACGAGGA AGATCGCACG
    CCAGATCGCA GCACATGGAA GTGGAGCCTT CACTCGGGAA GTAACTCGAC TCCAACTTAC
    CTGGAGCGAA GGAACGCCTG CCGGAGGAGT GAGATACGCC AGCGGAATTC AAAGTTCAAG
    CGGAAGACGG TGGCCAACTC CAGTTCCTTT GACAGTTTCA ATGAGTCCTT GGAACAGGAG
    GAGGAATCGA TTAGCCAGGC GAAGCCCTCG AAGCCACTGG CCAGAAATAA CAGCCTAGAC
    GATACTGCTC TACAGAAACT TGCTGCTGCT ATGAGATATC GCAAAAGAGA TGCTGCAGTG
    AAGGAGGAGA CGAAGACGCC GGTGAAGGAG CCTTGTAAGT GCGACCAGGA TGCGAAACAG
    GACGAGCGGA TTGAACCGTG TTCCTGCATT CTGCGGGATC AGCGCAGTCG CAGTGCCAAG
    TCACAATCTC CTGTCTTCAG CTACAAGGCA CTCAAGGAGC GCAGCAAGTC GGTTCCCCGG
    ATCGGAGGCT TTAGTTTGGA CGAGGAGGCT GAGAGAGAAC GGGAACAGGA CGAGGATAGC
    GCAGCCTCTC TGAGGGGCAG CAAGCCCGAT CTTAGCGAGC GCAAGACCAA GGGAAAGGGA
    TTCTTTGAAG TTAAGCAATA CAACCACAAG ACGATGCCCA AAAAGATAGC CAATTTGAAG
    AAGCAACGAA GTAGCACCAC CAAGAGCTGC TCCAGGTTCT ACATGGATCT CAGCGAATTC
    GATAGCAGCA GTTCGACTGT ACTCAAGATA ACCGAATCCA CCGAGGAGCT GCGTCCAGAT
    GGGGAAATCG AACTGGCCCA AGAAGCCGCC ACTCTGGATG AGTCCACGCA GGATCAGTAT
    TACAGCCCAG ATCACGAGAC GGAACTACTG TCCCCAGCAG AGAAGTCAAA GCGAGATGCA
    CAGATTGTCC TTGATTTGCT AAAGAATAGC AAGGAATTTG AGAGGATCTA CAACAAGCAA
    CAGAAGAGGC GCGAAAGTCC AGTGAGTCCC GTGAGTCCCG ATATAAGTCG TGGTCCCATT
    CTGCCGCCTC CGCGTCCACC ATCGAGATCT CCACCACCCT TGGAACTGGA GGAGCAGGAG
    AAGAAACCAG TCGAAGAGGT GGATTCAGTC AGCGAGGAGC CCATTTACGA GACTCTGCTG
    CGCAATGTCC ATGTGCCCTA CAAATTCTCA CCGGTGTTGG GCCGAGCCAA GTCCTCGCAG
    ATACTGAAGA AGCGATCAAA GACTGCTCCC GCAGCCTCAC GACCGGAATC GGATTACGTG
    ACCCTAGTCT ACTCGCCCGA AGGAGTTCTG CAGCGAGTGG GCGAGGAGGT GGTGCAGAGG
    GATAGCTGCA GTTCCTCCAC CACTTCGACA TCCTCAAATG GATCGGGGTC CACGCTGAAA
    CGAGACAGCC AGAGCACAGT GATCAATCTT TTGATGGAGG CGGCGCAAGA GCACGAATCA
    CGTCCCTTGC CGAAGAATTC AGTTTCAGGA TCGGAGAGTT CTCTGCTGCC ACGGGCTCTG
    AAGTCCATAG ACAATCTGAG CATGGCCTTC GGACGCTCCA ACCGACCGCC GGAGCGCCGG
    GTGTCCGATG TGAGCGAGAT GTGCCGTCAG AGTGTGCTTC ATCGCCAGGG CAGCGAGGCG
    GTGGGTGAGA GAATGGCCCA TGTGGATTAC GCTGATCCCA AGGCTCTATT TGGACTGGGG
    GTCCTGAACC ACTCGGAACG TGACTCGGTC TTCTCGCTGA CCTCCTCCTC CAGTGACAGC
    ATGTGCGAGC AGCAGCGCAA GCGATCCGGC GTGGGCGCCA CCTTCGAGTA TGAGCAGCAG
    GTGGAGGATA GCTTGGAGAA TGATTTTCGC GACTCGGCCA TTTACAGCGA TGACAACGAG
    AAGCGTTCCG GGGACTACGA TGTCCATCTA CGCCGTCCCA GCAGTCCGCC ACCACCACCG
    CCTCCGCTGG GGCCGCGTCC CCATCAGCCA CCACAGATTG CGCCGAAGCC TTCCAAGGAT
    CAGCTAATCC AGCAGCGATC GGGCGTCATC GTCTGCCAGT CGGCCTCCCG CAGCTGGGTG
    CTTCAGCAGA TTGAGAACTT CAACAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_788403.2
    CDS1046..5695
    Translation

    Target ORF information:

    RefSeq Version NM_176223.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster guanine nucleotide exchange factor in mesoderm, transcript variant E (GEFmeso), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_176223.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGACATAG AATCCGATTC GGACACCTCG GAGAGATGGG AGGACTTTTT TGGCAGCTCC 
    ACCGAATTGG CACCCTCGCT GCTGGGCATC TACGACACAT TGACAAACAG CTTTGCCGCG
    GGGGGCGTGG CAGCTGCTCC CACTGCGACA TCTCACGAAT CGAAGGCATC CAGCAGCGGC
    GACTCGACGC CCAAGAGGAG CAACAGTAAC TGCAGCAGCA GCAGCAGCAC TGGCTCATCG
    ACTGGCAGCT GTTCGAGTGT CCCCACCAGC GATGACCAGT CTGCTAGCTA CACGCTAGCC
    AGCTCCAGCA CTCATTTGGG CCACCAGGAG CCGCCCGCTC CACTGGTGGT TGCGGGCCTG
    CGGGACGAAC CGGATGGCGC TCAGTTGAGC AGGTTGGTGC AGCGCAGAAG CCAGGAGTCG
    CAGATTCACG GCAGCAGCAG CAGCATAGCC TCGAGTGGGA GCAGTCGACG GGGCAGCAAC
    ATCCGCATCC TTTCGCCCAA TGTTCATAGA ATCATCACGC ACTCGGATAT TCAACCAGTG
    GAGGCGGTGT CCGCGGAGGC ACTGAAACAG CATCAGCAGC AATCCCAGAG GAGTCCCACC
    CAGGGCAGGT ATATAAAGAC CGGTCATAGC GGAACACCAC CAGCCGCTGG TTCCGCACCC
    AAGCTGAAGC TCTCGCACAT ACCGCTGTCC AAGATCACCG GCAAGTTGTC CACCCAGTCT
    GGCAGCGAAC TGGTGTCGAC CTTCGATTCC AGCAACGAGT ATTTGAACTC GGTGGCCTTC
    GCCGCTTTCG AGAAATCCAA GCCGCAGGAC GAGGATCGGA CGCCAACGAA CAAATCGGTA
    CCAGGCACTC CCTTCCATTT CGATGGGCAT CCGTTTCAGG GGCGAAAACT AACCCTACCA
    CGGTACGATT CACAGCAGAG CATCAAGCTG ATCGAAATGG AGCAGCTGGC GGCCACCAGT
    GCGCAACTGT TGCATGCCAA GCCTGCCACG CCCGGAACTC CGGCCACGCC CACTCCCAGT
    GCAACCAAAG CCAAGGAGTT TGTGTGCAGT GAGCCACTGA GTCCCGTGGA TATAGTGGAC
    ACCATTAACT TCGATCTGGT TGCGCAGCAT ATGGAAAGAT TGACTCTGGC GGATCCAGGA
    GATTTGGATC CCAGGGCGGA GCTAATAGAA GCGGTTAACA AAACACTGGT TAACAAGCCA
    CTGGTTAGGA GCTCCTCCGC CGCCTGTGCC TCCACAATAT GCAGTTCACC GCTGTTGACC
    TATGCCCGCA CCAAGAGTTT GGGCGCCAAG GCCAGCACTT TGGGCGGTGG AGTGCCTATT
    AAGCTGCCCA CTGCCGAACA GCTGGCCGAG CTGGAGGAGG CCAACAAGTT GTCGGCGGAA
    AGTCTGGACA GGCTCACCGA TATCAAGCCC AAGTTTGCAG CTCGCCTAAG TGAGCTGGCT
    AGGCTGAATA ACCGCCCACT GTCCTCCTCC TCCATTTGCT CCACCTCCTC CAGTTCCAGT
    TCCGGTTCGG ACCAACTGCT CAATGGAAAA CTGATGGCCA CTTCCTACTT GGCTAGCGTA
    GAAAGTCTGG CGGAGAGTGA GAACGAGCTG GGCGATCAAC ACCATCCGCC GGCGATGAGT
    GTCCTCGAGA AAACCTGCCT AGAGATCGTA GACTCGGAGC GAAGCTTCGT CGAGGATTTG
    GGTCAGGTCA TCAAGGGATA CCTGCAGGAC TGGAAGGAGC GTGCGTGCCT GCGCGTAGAC
    GAATTGCAAA TACTCTTCGC CAACATTGAA GAGATTTATG AATTCAACTC AATGCTTCTG
    CAGCGACTTA TCAACACGGG AAGGGATCCC GGGAGGATCG CCAGGTGCTT CATTGACCTG
    CGCGATGGCT TTGATGTGTA CACAACCTAC TGCACAAGCT ACCCGGAAGC CATATCGTTG
    CTGACCAAGT TGCTGCAGGC CACGCACACG TACTCTCTGC TTGCCTCCAC GCAGAAGCTA
    CTCCAACATC GCCTGCCCCT GGGATCCTAC CTCCTGAAAC CGGTGCAGCG CATCCTCAAG
    TACCATCTGC TGCTGGACAG CCTGCGCAAG CACTGCGATG TGAAGGAGGT GGTCGAGGCC
    CATGTGATCA TGCGCCAGGT GGCGCACAAC ATTGACCAGG TGAAGCGAAA GCAGGAGCAG
    CAGAGTCGCG TCAAGGAGCT GTCCGGCATT CTGGACGGCT GGCTGGGGCC AGAGCTCACT
    GTTCTGGGAG AGTTGCGCCA AGAGGGCCTG CTGATGGAGC AGCACAATAA ACAGCGCCTG
    GTACTGCTAT TCGCCACCAT GCTGATCATC ACCAAGCAAA AGGAGGACGG ACGCCTTCAG
    TTCAAGACTT ACATTTCGCA AAACAATCTG ATGCTGAGCG AACACTTGCC CGGTGAACCG
    ACCAGCTTCT ATGTCATCCC CTTCGACGAG CCGCGCCATC AAATCAAACT GACGGCTCGG
    AATCGTGACC AGAAGCGCAT CTGGACGCAG CACATCAAAG GTGTCATGCT GGAGAAGTTA
    GACATTCCGA TGCGCGCCAA GGAGCTGGTC TATCAGCTGG GCAACGAGGA AGATCGCACG
    CCAGATCGCA GCACATGGAA GTGGAGCCTT CACTCGGGAA GTAACTCGAC TCCAACTTAC
    CTGGAGCGAA GGAACGCCTG CCGGAGGAGT GAGATACGCC AGCGGAATTC AAAGTTCAAG
    CGGAAGACGG TGGCCAACTC CAGTTCCTTT GACAGTTTCA ATGAGTCCTT GGAACAGGAG
    GAGGAATCGA TTAGCCAGGC GAAGCCCTCG AAGCCACTGG CCAGAAATAA CAGCCTAGAC
    GATACTGCTC TACAGAAACT TGCTGCTGCT ATGAGATATC GCAAAAGAGA TGCTGCAGTG
    AAGGAGGAGA CGAAGACGCC GGTGAAGGAG CCTTGTAAGT GCGACCAGGA TGCGAAACAG
    GACGAGCGGA TTGAACCGTG TTCCTGCATT CTGCGGGATC AGCGCAGTCG CAGTGCCAAG
    TCACAATCTC CTGTCTTCAG CTACAAGGCA CTCAAGGAGC GCAGCAAGTC GGTTCCCCGG
    ATCGGAGGCT TTAGTTTGGA CGAGGAGGCT GAGAGAGAAC GGGAACAGGA CGAGGATAGC
    GCAGCCTCTC TGAGGGGCAG CAAGCCCGAT CTTAGCGAGC GCAAGACCAA GGGAAAGGGA
    TTCTTTGAAG TTAAGCAATA CAACCACAAG ACGATGCCCA AAAAGATAGC CAATTTGAAG
    AAGCAACGAA GTAGCACCAC CAAGAGCTGC TCCAGGTTCT ACATGGATCT CAGCGAATTC
    GATAGCAGCA GTTCGACTGT ACTCAAGATA ACCGAATCCA CCGAGGAGCT GCGTCCAGAT
    GGGGAAATCG AACTGGCCCA AGAAGCCGCC ACTCTGGATG AGTCCACGCA GGATCAGTAT
    TACAGCCCAG ATCACGAGAC GGAACTACTG TCCCCAGCAG AGAAGTCAAA GCGAGATGCA
    CAGATTGTCC TTGATTTGCT AAAGAATAGC AAGGAATTTG AGAGGATCTA CAACAAGCAA
    CAGAAGAGGC GCGAAAGTCC AGTGAGTCCC GTGAGTCCCG ATATAAGTCG TGGTCCCATT
    CTGCCGCCTC CGCGTCCACC ATCGAGATCT CCACCACCCT TGGAACTGGA GGAGCAGGAG
    AAGAAACCAG TCGAAGAGGT GGATTCAGTC AGCGAGGAGC CCATTTACGA GACTCTGCTG
    CGCAATGTCC ATGTGCCCTA CAAATTCTCA CCGGTGTTGG GCCGAGCCAA GTCCTCGCAG
    ATACTGAAGA AGCGATCAAA GACTGCTCCC GCAGCCTCAC GACCGGAATC GGATTACGTG
    ACCCTAGTCT ACTCGCCCGA AGGAGTTCTG CAGCGAGTGG GCGAGGAGGT GGTGCAGAGG
    GATAGCTGCA GTTCCTCCAC CACTTCGACA TCCTCAAATG GATCGGGGTC CACGCTGAAA
    CGAGACAGCC AGAGCACAGT GATCAATCTT TTGATGGAGG CGGCGCAAGA GCACGAATCA
    CGTCCCTTGC CGAAGAATTC AGTTTCAGGA TCGGAGAGTT CTCTGCTGCC ACGGGCTCTG
    AAGTCCATAG ACAATCTGAG CATGGCCTTC GGACGCTCCA ACCGACCGCC GGAGCGCCGG
    GTGTCCGATG TGAGCGAGAT GTGCCGTCAG AGTGTGCTTC ATCGCCAGGG CAGCGAGGCG
    GTGGGTGAGA GAATGGCCCA TGTGGATTAC GCTGATCCCA AGGCTCTATT TGGACTGGGG
    GTCCTGAACC ACTCGGAACG TGACTCGGTC TTCTCGCTGA CCTCCTCCTC CAGTGACAGC
    ATGTGCGAGC AGCAGCGCAA GCGATCCGGC GTGGGCGCCA CCTTCGAGTA TGAGCAGCAG
    GTGGAGGATA GCTTGGAGAA TGATTTTCGC GACTCGGCCA TTTACAGCGA TGACAACGAG
    AAGCGTTCCG GGGACTACGA TGTCCATCTA CGCCGTCCCA GCAGTCCGCC ACCACCACCG
    CCTCCGCTGG GGCCGCGTCC CCATCAGCCA CCACAGATTG CGCCGAAGCC TTCCAAGGAT
    CAGCTAATCC AGCAGCGATC GGGCGTCATC GTCTGCCAGT CGGCCTCCCG CAGCTGGGTG
    CTTCAGCAGA TTGAGAACTT CAACAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.