GEFmeso cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	GEFmeso
Entrez Gene ID	37134
Full Name	Guanine nucleotide exchange factor in mesoderm
Synonyms	CG18430,CG30115,CG5503,Dmel\CG30115
General protein information	Preferred Names Guanine nucleotide exchange factor in mesoderm Names CG30115 gene product from transcript CG30115-RE CG30115-PE CG30115-PF CG30115-PG GEFmeso-PE GEFmeso-PF GEFmeso-PG guanine nucleotide exchange factor in mesoderm
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 55D3-55E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_176223.4	NP_788403.2	guanine nucleotide exchange factor in mesoderm, isoform E
NM_001274146.2	NP_001261075.1	guanine nucleotide exchange factor in mesoderm, isoform F
NM_137499.3	NP_611343.4	guanine nucleotide exchange factor in mesoderm, isoform G

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We propose that the guanine nucleotide exchange factor GEFmeso is involved in a developmental process that requires the synergistic action of CDC42 and Rac1 during Drosophila development
Title: Rac1 is a novel interactor of Drosophila guanine nucleotide exchange factor GEFmeso.

The identification and characterization of a DBL-like guanine nucleotide exchange factor (GEF) in Drosophila, called GEFmeso, as a novel binding target of the Ras-like GTPase Ral.
Title: Novel guanine nucleotide exchange factor GEFmeso of Drosophila melanogaster interacts with Ral and Rho GTPase Cdc42.

The following GEFmeso gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GEFmeso cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa12188	NM_001274146.2 Latest version!	Drosophila melanogaster guanine nucleotide exchange factor in mesoderm, transcript variant F (GEFmeso), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa21852	NM_137499.3 Latest version!	Drosophila melanogaster guanine nucleotide exchange factor in mesoderm, transcript variant G (GEFmeso), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa10503	NM_176223.4 Latest version!	Drosophila melanogaster guanine nucleotide exchange factor in mesoderm, transcript variant E (GEFmeso), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GEFmeso ( NM_001274146.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261075.1 Drosophila melanogaster guanine nucleotide exchange factor in mesoderm, transcript variant F (GEFmeso), mRNA.

Price & Availability↑

CloneID	OFa12188
Clone ID Related Accession (Same CDS sequence)	NM_001274146.2
Accession Version	NM_001274146.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2637bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	guanine nucleotide exchange factor in mesoderm, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001274146.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACATAG AATCCGATTC GGACACCTCG GAGAGATGGG AGGACTTTTT TGGCAGCTCC 
ACCGAATTGG CACCCTCGCT GCTGGGCATC TACGACACAT TGACAAACAG CTTTGCCGCG 
GGGGGCGTGG CAGCTGCTCC CACTGCGACA TCTCACGAAT CGAAGGCATC CAGCAGCGGC 
GACTCGACGC CCAAGAGGAG CAACAGTAAC TGCAGCAGCA GCAGCAGCAC TGGCTCATCG 
ACTGGCAGCT GTTCGAGTGT CCCCACCAGC GATGACCAGT CTGCTAGCTA CACGCTAGCC 
AGCTCCAGCA CTCATTTGGG CCACCAGGAG CCGCCCGCTC CACTGGTGGT TGCGGGCCTG 
CGGGACGAAC CGGATGGCGC TCAGTTGAGC AGGTTGGTGC AGCGCAGAAG CCAGGAGTCG 
CAGATTCACG GCAGCAGCAG CAGCATAGCC TCGAGTGGGA GCAGTCGACG GGGCAGCAAC 
ATCCGCATCC TTTCGCCCAA TGTTCATAGA ATCATCACGC ACTCGGATAT TCAACCAGTG 
GAGGCGGTGT CCGCGGAGGC ACTGAAACAG CATCAGCAGC AATCCCAGAG GAGTCCCACC 
CAGGGCAGGT ATATAAAGAC CGGTCATAGC GGAACACCAC CAGCCGCTGG TTCCGCACCC 
AAGCTGAAGC TCTCGCACAT ACCGCTGTCC AAGATCACCG GCAAGTTGTC CACCCAGTCT 
GGCAGCGAAC TGGTGTCGAC CTTCGATTCC AGCAACGAGT ATTTGAACTC GGTGGCCTTC 
GCCGCTTTCG AGAAATCCAA GCCGCAGGAC GAGGATCGGA CGCCAACGAA CAAATCGGTA 
CCAGGCACTC CCTTCCATTT CGATGGGCAT CCGTTTCAGG GGCGAAAACT AACCCTACCA 
CGGTACGATT CACAGCAGAG CATCAAGCTG ATCGAAATGG AGCAGCTGGC GGCCACCAGT 
GCGCAACTGT TGCATGCCAA GCCTGCCACG CCCGGAACTC CGGCCACGCC CACTCCCAGT 
GCAACCAAAG CCAAGGAGTT TGTGTGCAGT GAGCCACTGA GTCCCGTGGA TATAGTGGAC 
ACCATTAACT TCGATCTGGT TGCGCAGCAT ATGGAAAGAT TGACTCTGGC GGATCCAGGA 
GATTTGGATC CCAGGGCGGA GCTAATAGAA GCGGTTAACA AAACACTGGT TAACAAGCCA 
CTGGTTAGGA GCTCCTCCGC CGCCTGTGCC TCCACAATAT GCAGTTCACC GCTGTTGACC 
TATGCCCGCA CCAAGAGTTT GGGCGCCAAG GCCAGCACTT TGGGCGGTGG AGTGCCTATT 
AAGCTGCCCA CTGCCGAACA GCTGGCCGAG CTGGAGGAGG CCAACAAGTT GTCGGCGGAA 
AGTCTGGACA GGCTCACCGA TATCAAGCCC AAGTTTGCAG CTCGCCTAAG TGAGCTGGCT 
AGGCTGAATA ACCGCCCACT GTCCTCCTCC TCCATTTGCT CCACCTCCTC CAGTTCCAGT 
TCCGGTTCGG ACCAACTGCT CAATGGAAAA CTGATGGCCA CTTCCTACTT GGCTAGCGTA 
GAAAGTCTGG CGGAGAGTGA GAACGAGCTG GGCGATCAAC ACCATCCGCC GGCGATGAGT 
GTCCTCGAGA AAACCTGCCT AGAGATCGTA GACTCGGAGC GAAGCTTCGT CGAGGATTTG 
GGTCAGGTCA TCAAGGGATA CCTGCAGGAC TGGAAGGAGC GTGCGTGCCT GCGCGTAGAC 
GAATTGCAAA TACTCTTCGC CAACATTGAA GAGATTTATG AATTCAACTC AATGCTTCTG 
CAGCGACTTA TCAACACGGG AAGGGATCCC GGGAGGATCG CCAGGTGCTT CATTGACCTG 
CGCGATGGCT TTGATGTGTA CACAACCTAC TGCACAAGCT ACCCGGAAGC CATATCGTTG 
CTGACCAAGT TGCTGCAGGC CACGCACACG TACTCTCTGC TTGCCTCCAC GCAGAAGCTA 
CTCCAACATC GCCTGCCCCT GGGATCCTAC CTCCTGAAAC CGGTGCAGCG CATCCTCAAG 
TACCATCTGC TGCTGGACAG CCTGCGCAAG CACTGCGATG TGAAGGAGGT GGTCGAGGCC 
CATGTGATCA TGCGCCAGGT GGCGCACAAC ATTGACCAGG TGAAGCGAAA GCAGGAGCAG 
CAGAGTCGCG TCAAGGAGCT GTCCGGCATT CTGGACGGCT GGCTGGGGCC AGAGCTCACT 
GTTCTGGGAG AGTTGCGCCA AGAGGGCCTG CTGATGGAGC AGCACAATAA ACAGCGCCTG 
GTACTGCTAT TCGCCACCAT GCTGATCATC ACCAAGCAAA AGGAGGACGG ACGCCTTCAG 
TTCAAGACTT ACATTTCGCA AAACAATCTG ATGCTGAGCG AACACTTGCC CGGTGAACCG 
ACCAGCTTCT ATGTCATCCC CTTCGACGAG CCGCGCCATC AAATCAAACT GACGGCTCGG 
AATCGTGACC AGAAGCGCAT CTGGACGCAG CACATCAAAG GTGTCATGCT GGAGAAGTTA 
GACATTCCGA TGCGCGCCAA GGAGCTGGTC TATCAGCTGG GCAACGAGGA AGTACTACCA 
GTGATGCATT CTAAAACTAA ACACCCCAAA ACAAAGAAAC GAAAAAACTT TGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261075.1
CDS	1046..3682
Translation	MDIESDSDTSERWEDFFGSSTELAPSLLGIYDTLTNSFAAGGVAAAPTATSHESKASSSGDSTPKRSNSNCSSSSSTGSSTGSCSSVPTSDDQSASYTLASSSTHLGHQEPPAPLVVAGLRDEPDGAQLSRLVQRRSQESQIHGSSSSIASSGSSRRGSNIRILSPNVHRIITHSDIQPVEAVSAEALKQHQQQSQRSPTQGRYIKTGHSGTPPAAGSAPKLKLSHIPLSKITGKLSTQSGSELVSTFDSSNEYLNSVAFAAFEKSKPQDEDRTPTNKSVPGTPFHFDGHPFQGRKLTLPRYDSQQSIKLIEMEQLAATSAQLLHAKPATPGTPATPTPSATKAKEFVCSEPLSPVDIVDTINFDLVAQHMERLTLADPGDLDPRAELIEAVNKTLVNKPLVRSSSAACASTICSSPLLTYARTKSLGAKASTLGGGVPIKLPTAEQLAELEEANKLSAESLDRLTDIKPKFAARLSELARLNNRPLSSSSICSTSSSSSSGSDQLLNGKLMATSYLASVESLAESENELGDQHHPPAMSVLEKTCLEIVDSERSFVEDLGQVIKGYLQDWKERACLRVDELQILFANIEEIYEFNSMLLQRLINTGRDPGRIARCFIDLRDGFDVYTTYCTSYPEAISLLTKLLQATHTYSLLASTQKLLQHRLPLGSYLLKPVQRILKYHLLLDSLRKHCDVKEVVEAHVIMRQVAHNIDQVKRKQEQQSRVKELSGILDGWLGPELTVLGELRQEGLLMEQHNKQRLVLLFATMLIITKQKEDGRLQFKTYISQNNLMLSEHLPGEPTSFYVIPFDEPRHQIKLTARNRDQKRIWTQHIKGVMLEKLDIPMRAKELVYQLGNEEVLPVMHSKTKHPKTKKRKNFD

Target ORF information:

RefSeq Version	NM_001274146.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster guanine nucleotide exchange factor in mesoderm, transcript variant F (GEFmeso), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274146.2

ORF Insert Sequence:

ATGGACATAG AATCCGATTC GGACACCTCG GAGAGATGGG AGGACTTTTT TGGCAGCTCC 
ACCGAATTGG CACCCTCGCT GCTGGGCATC TACGACACAT TGACAAACAG CTTTGCCGCG 
GGGGGCGTGG CAGCTGCTCC CACTGCGACA TCTCACGAAT CGAAGGCATC CAGCAGCGGC 
GACTCGACGC CCAAGAGGAG CAACAGTAAC TGCAGCAGCA GCAGCAGCAC TGGCTCATCG 
ACTGGCAGCT GTTCGAGTGT CCCCACCAGC GATGACCAGT CTGCTAGCTA CACGCTAGCC 
AGCTCCAGCA CTCATTTGGG CCACCAGGAG CCGCCCGCTC CACTGGTGGT TGCGGGCCTG 
CGGGACGAAC CGGATGGCGC TCAGTTGAGC AGGTTGGTGC AGCGCAGAAG CCAGGAGTCG 
CAGATTCACG GCAGCAGCAG CAGCATAGCC TCGAGTGGGA GCAGTCGACG GGGCAGCAAC 
ATCCGCATCC TTTCGCCCAA TGTTCATAGA ATCATCACGC ACTCGGATAT TCAACCAGTG 
GAGGCGGTGT CCGCGGAGGC ACTGAAACAG CATCAGCAGC AATCCCAGAG GAGTCCCACC 
CAGGGCAGGT ATATAAAGAC CGGTCATAGC GGAACACCAC CAGCCGCTGG TTCCGCACCC 
AAGCTGAAGC TCTCGCACAT ACCGCTGTCC AAGATCACCG GCAAGTTGTC CACCCAGTCT 
GGCAGCGAAC TGGTGTCGAC CTTCGATTCC AGCAACGAGT ATTTGAACTC GGTGGCCTTC 
GCCGCTTTCG AGAAATCCAA GCCGCAGGAC GAGGATCGGA CGCCAACGAA CAAATCGGTA 
CCAGGCACTC CCTTCCATTT CGATGGGCAT CCGTTTCAGG GGCGAAAACT AACCCTACCA 
CGGTACGATT CACAGCAGAG CATCAAGCTG ATCGAAATGG AGCAGCTGGC GGCCACCAGT 
GCGCAACTGT TGCATGCCAA GCCTGCCACG CCCGGAACTC CGGCCACGCC CACTCCCAGT 
GCAACCAAAG CCAAGGAGTT TGTGTGCAGT GAGCCACTGA GTCCCGTGGA TATAGTGGAC 
ACCATTAACT TCGATCTGGT TGCGCAGCAT ATGGAAAGAT TGACTCTGGC GGATCCAGGA 
GATTTGGATC CCAGGGCGGA GCTAATAGAA GCGGTTAACA AAACACTGGT TAACAAGCCA 
CTGGTTAGGA GCTCCTCCGC CGCCTGTGCC TCCACAATAT GCAGTTCACC GCTGTTGACC 
TATGCCCGCA CCAAGAGTTT GGGCGCCAAG GCCAGCACTT TGGGCGGTGG AGTGCCTATT 
AAGCTGCCCA CTGCCGAACA GCTGGCCGAG CTGGAGGAGG CCAACAAGTT GTCGGCGGAA 
AGTCTGGACA GGCTCACCGA TATCAAGCCC AAGTTTGCAG CTCGCCTAAG TGAGCTGGCT 
AGGCTGAATA ACCGCCCACT GTCCTCCTCC TCCATTTGCT CCACCTCCTC CAGTTCCAGT 
TCCGGTTCGG ACCAACTGCT CAATGGAAAA CTGATGGCCA CTTCCTACTT GGCTAGCGTA 
GAAAGTCTGG CGGAGAGTGA GAACGAGCTG GGCGATCAAC ACCATCCGCC GGCGATGAGT 
GTCCTCGAGA AAACCTGCCT AGAGATCGTA GACTCGGAGC GAAGCTTCGT CGAGGATTTG 
GGTCAGGTCA TCAAGGGATA CCTGCAGGAC TGGAAGGAGC GTGCGTGCCT GCGCGTAGAC 
GAATTGCAAA TACTCTTCGC CAACATTGAA GAGATTTATG AATTCAACTC AATGCTTCTG 
CAGCGACTTA TCAACACGGG AAGGGATCCC GGGAGGATCG CCAGGTGCTT CATTGACCTG 
CGCGATGGCT TTGATGTGTA CACAACCTAC TGCACAAGCT ACCCGGAAGC CATATCGTTG 
CTGACCAAGT TGCTGCAGGC CACGCACACG TACTCTCTGC TTGCCTCCAC GCAGAAGCTA 
CTCCAACATC GCCTGCCCCT GGGATCCTAC CTCCTGAAAC CGGTGCAGCG CATCCTCAAG 
TACCATCTGC TGCTGGACAG CCTGCGCAAG CACTGCGATG TGAAGGAGGT GGTCGAGGCC 
CATGTGATCA TGCGCCAGGT GGCGCACAAC ATTGACCAGG TGAAGCGAAA GCAGGAGCAG 
CAGAGTCGCG TCAAGGAGCT GTCCGGCATT CTGGACGGCT GGCTGGGGCC AGAGCTCACT 
GTTCTGGGAG AGTTGCGCCA AGAGGGCCTG CTGATGGAGC AGCACAATAA ACAGCGCCTG 
GTACTGCTAT TCGCCACCAT GCTGATCATC ACCAAGCAAA AGGAGGACGG ACGCCTTCAG 
TTCAAGACTT ACATTTCGCA AAACAATCTG ATGCTGAGCG AACACTTGCC CGGTGAACCG 
ACCAGCTTCT ATGTCATCCC CTTCGACGAG CCGCGCCATC AAATCAAACT GACGGCTCGG 
AATCGTGACC AGAAGCGCAT CTGGACGCAG CACATCAAAG GTGTCATGCT GGAGAAGTTA 
GACATTCCGA TGCGCGCCAA GGAGCTGGTC TATCAGCTGG GCAACGAGGA AGTACTACCA 
GTGATGCATT CTAAAACTAA ACACCCCAAA ACAAAGAAAC GAAAAAACTT TGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GEFmeso ( NM_137499.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611343.4 Drosophila melanogaster guanine nucleotide exchange factor in mesoderm, transcript variant G (GEFmeso), mRNA.

Price & Availability↑

CloneID	OFa21852
Clone ID Related Accession (Same CDS sequence)	NM_137499.3
Accession Version	NM_137499.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	guanine nucleotide exchange factor in mesoderm, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137499.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGCAGC TGGCGGCCAC CAGTGCGCAA CTGTTGCATG CCAAGCCTGC CACGCCCGGA 
ACTCCGGCCA CGCCCACTCC CAGTGCAACC AAAGCCAAGG AGTTTGTGTG CAGTGAGCCA 
CTGAGTCCCG TGGATATAGT GGACACCATT AACTTCGATC TGGTTGCGCA GCATATGGAA 
AGATTGACTC TGGCGGATCC AGGAGATTTG GATCCCAGGG CGGAGCTAAT AGAAGCGGTT 
AACAAAACAC TGGTTAACAA GCCACTGGTT AGGAGCTCCT CCGCCGCCTG TGCCTCCACA 
ATATGCAGTT CACCGCTGTT GACCTATGCC CGCACCAAGA GTTTGGGCGC CAAGGCCAGC 
ACTTTGGGCG GTGGAGTGCC TATTAAGCTG CCCACTGCCG AACAGCTGGC CGAGCTGGAG 
GAGGCCAACA AGTTGTCGGC GGAAAGTCTG GACAGGCTCA CCGATATCAA GCCCAAGTTT 
GCAGCTCGCC TAAGTGAGCT GGCTAGGCTG AATAACCGCC CACTGTCCTC CTCCTCCATT 
TGCTCCACCT CCTCCAGTTC CAGTTCCGGT TCGGACCAAC TGCTCAATGG AAAACTGATG 
GCCACTTCCT ACTTGGCTAG CGTAGAAAGT CTGGCGGAGA GTGAGAACGA GCTGGGCGAT 
CAACACCATC CGCCGGCGAT GAGTGTCCTC GAGAAAACCT GCCTAGAGAT CGTAGACTCG 
GAGCGAAGCT TCGTCGAGGA TTTGGGTCAG GTCATCAAGG GATACCTGCA GGACTGGAAG 
GAGCGTGCGT GCCTGCGCGT AGACGAATTG CAAATACTCT TCGCCAACAT TGAAGAGATT 
TATGAATTCA ACTCAATGCT TCTGCAGCGA CTTATCAACA CGGGAAGGGA TCCCGGGAGG 
ATCGCCAGGT GCTTCATTGA CCTGCGCGAT GGCTTTGATG TGTACACAAC CTACTGCACA 
AGCTACCCGG AAGCCATATC GTTGCTGACC AAGTTGCTGC AGGCCACGCA CACGTACTCT 
CTGCTTGCCT CCACGCAGAA GCTACTCCAA CATCGCCTGC CCCTGGGATC CTACCTCCTG 
AAACCGGTGC AGCGCATCCT CAAGTACCAT CTGCTGCTGG ACAGCCTGCG CAAGCACTGC 
GATGTGAAGG AGGTGGTCGA GGCCCATGTG ATCATGCGCC AGGTGGCGCA CAACATTGAC 
CAGGTGAAGC GAAAGCAGGA GCAGCAGAGT CGCGTCAAGG AGCTGTCCGG CATTCTGGAC 
GGCTGGCTGG GGCCAGAGCT CACTGTTCTG GGAGAGTTGC GCCAAGAGGG CCTGCTGATG 
GAGCAGCACA ATAAACAGCG CCTGGTACTG CTATTCGCCA CCATGCTGAT CATCACCAAG 
CAAAAGGAGG ACGGACGCCT TCAGTTCAAG ACTTACATTT CGCAAAACAA TCTGATGCTG 
AGCGAACACT TGCCCGGTGA ACCGACCAGC TTCTATGTCA TCCCCTTCGA CGAGCCGCGC 
CATCAAATCA AACTGACGGC TCGGAATCGT GACCAGAAGC GCATCTGGAC GCAGCACATC 
AAAGGTGTCA TGCTGGAGAA GTTAGACATT CCGATGCGCG CCAAGGAGCT GGTCTATCAG 
CTGGGCAACG AGGAAGATCG CACGCCAGAT CGCAGCACAT GGAAGTGGAG CCTTCACTCG 
GGAAGTAACT CGACTCCAAC TTACCTGGAG CGAAGGAACG CCTGCCGGAG GAGTGAGATA 
CGCCAGCGGA ATTCAAAGTT CAAGCGGAAG ACGGTGGCCA ACTCCAGTTC CTTTGACAGT 
TTCAATGAGT CCTTGGAACA GGAGGAGGAA TCGATTAGCC AGGCGAAGCC CTCGAAGCCA 
CTGGCCAGAA ATAACAGCCT AGACGATACT GCTCTACAGA AACTTGCTGC TGCTATGAGA 
TATCGCAAAA GAGATGCTGC AGTGAAGGAG GAGACGAAGA CGCCGGTGAA GGAGCCTTGT 
AAGTGCGACC AGGATGCGAA ACAGGACGAG CGGATTGAAC CGTGTTCCTG CATTCTGCGG 
GATCAGCGCA GTCGCAGTGC CAAGTCACAA TCTCCTGTCT TCAGCTACAA GGCACTCAAG 
GAGCGCAGCA AGTCGGTTCC CCGGATCGGA GGCTTTAGTT TGGACGAGGA GGCTGAGAGA 
GAACGGGAAC AGGACGAGGA TAGCGCAGCC TCTCTGAGGG GCAGCAAGCC CGATCTTAGC 
GAGCGCAAGA CCAAGGGAAA GGGATTCTTT GAAGTTAAGC AATACAACCA CAAGACGATG 
CCCAAAAAGA TAGCCAATTT GAAGAAGCAA CGAAGTAGCA CCACCAAGAG CTGCTCCAGG 
TTCTACATGG ATCTCAGCGA ATTCGATAGC AGCAGTTCGA CTGTACTCAA GATAACCGAA 
TCCACCGAGG AGCTGCGTCC AGATGGGGAA ATCGAACTGG CCCAAGAAGC CGCCACTCTG 
GATGAGTCCA CGCAGGATCA GTATTACAGC CCAGATCACG AGACGGAACT ACTGTCCCCA 
GCAGAGAAGT CAAAGCGAGA TGCACAGATT GTCCTTGATT TGCTAAAGAA TAGCAAGGAA 
TTTGAGAGGA TCTACAACAA GCAACAGAAG AGGCGCGAAA GTCCAGTGAG TCCCGTGAGT 
CCCGATATAA GTCGTGGTCC CATTCTGCCG CCTCCGCGTC CACCATCGAG ATCTCCACCA 
CCCTTGGAAC TGGAGGAGCA GGAGAAGAAA CCAGTCGAAG AGGTGGATTC AGTCAGCGAG 
GAGCCCATTT ACGAGACTCT GCTGCGCAAT GTCCATGTGC CCTACAAATT CTCACCGGTG 
TTGGGCCGAG CCAAGTCCTC GCAGATACTG AAGAAGCGAT CAAAGACTGC TCCCGCAGCC 
TCACGACCGG AATCGGATTA CGTGACCCTA GTCTACTCGC CCGAAGGAGT TCTGCAGCGA 
GTGGGCGAGG AGGTGGTGCA GAGGGATAGC TGCAGTTCCT CCACCACTTC GACATCCTCA 
AATGGATCGG GGTCCACGCT GAAACGAGAC AGCCAGAGCA CAGTGATCAA TCTTTTGATG 
GAGGCGGCGC AAGAGCACGA ATCACGTCCC TTGCCGAAGA ATTCAGTTTC AGGATCGGAG 
AGTTCTCTGC TGCCACGGGC TCTGAAGTCC ATAGACAATC TGAGCATGGC CTTCGGACGC 
TCCAACCGAC CGCCGGAGCG CCGGGTGTCC GATGTGAGCG AGATGTGCCG TCAGAGTGTG 
CTTCATCGCC AGGGCAGCGA GGCGGTGGGT GAGAGAATGG CCCATGTGGA TTACGCTGAT 
CCCAAGGCTC TATTTGGACT GGGGGTCCTG AACCACTCGG AACGTGACTC GGTCTTCTCG 
CTGACCTCCT CCTCCAGTGA CAGCATGTGC GAGCAGCAGC GCAAGCGATC CGGCGTGGGC 
GCCACCTTCG AGTATGAGCA GCAGGTGGAG GATAGCTTGG AGAATGATTT TCGCGACTCG 
GCCATTTACA GCGATGACAA CGAGAAGCGT TCCGGGGACT ACGATGTCCA TCTACGCCGT 
CCCAGCAGTC CGCCACCACC ACCGCCTCCG CTGGGGCCGC GTCCCCATCA GCCACCACAG 
ATTGCGCCGA AGCCTTCCAA GGATCAGCTA ATCCAGCAGC GATCGGGCGT CATCGTCTGC 
CAGTCGGCCT CCCGCAGCTG GGTGCTTCAG CAGATTGAGA ACTTCAACAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611343.4
CDS	1673..5386
Translation	MEQLAATSAQLLHAKPATPGTPATPTPSATKAKEFVCSEPLSPVDIVDTINFDLVAQHMERLTLADPGDLDPRAELIEAVNKTLVNKPLVRSSSAACASTICSSPLLTYARTKSLGAKASTLGGGVPIKLPTAEQLAELEEANKLSAESLDRLTDIKPKFAARLSELARLNNRPLSSSSICSTSSSSSSGSDQLLNGKLMATSYLASVESLAESENELGDQHHPPAMSVLEKTCLEIVDSERSFVEDLGQVIKGYLQDWKERACLRVDELQILFANIEEIYEFNSMLLQRLINTGRDPGRIARCFIDLRDGFDVYTTYCTSYPEAISLLTKLLQATHTYSLLASTQKLLQHRLPLGSYLLKPVQRILKYHLLLDSLRKHCDVKEVVEAHVIMRQVAHNIDQVKRKQEQQSRVKELSGILDGWLGPELTVLGELRQEGLLMEQHNKQRLVLLFATMLIITKQKEDGRLQFKTYISQNNLMLSEHLPGEPTSFYVIPFDEPRHQIKLTARNRDQKRIWTQHIKGVMLEKLDIPMRAKELVYQLGNEEDRTPDRSTWKWSLHSGSNSTPTYLERRNACRRSEIRQRNSKFKRKTVANSSSFDSFNESLEQEEESISQAKPSKPLARNNSLDDTALQKLAAAMRYRKRDAAVKEETKTPVKEPCKCDQDAKQDERIEPCSCILRDQRSRSAKSQSPVFSYKALKERSKSVPRIGGFSLDEEAEREREQDEDSAASLRGSKPDLSERKTKGKGFFEVKQYNHKTMPKKIANLKKQRSSTTKSCSRFYMDLSEFDSSSSTVLKITESTEELRPDGEIELAQEAATLDESTQDQYYSPDHETELLSPAEKSKRDAQIVLDLLKNSKEFERIYNKQQKRRESPVSPVSPDISRGPILPPPRPPSRSPPPLELEEQEKKPVEEVDSVSEEPIYETLLRNVHVPYKFSPVLGRAKSSQILKKRSKTAPAASRPESDYVTLVYSPEGVLQRVGEEVVQRDSCSSSTTSTSSNGSGSTLKRDSQSTVINLLMEAAQEHESRPLPKNSVSGSESSLLPRALKSIDNLSMAFGRSNRPPERRVSDVSEMCRQSVLHRQGSEAVGERMAHVDYADPKALFGLGVLNHSERDSVFSLTSSSSDSMCEQQRKRSGVGATFEYEQQVEDSLENDFRDSAIYSDDNEKRSGDYDVHLRRPSSPPPPPPPLGPRPHQPPQIAPKPSKDQLIQQRSGVIVCQSASRSWVLQQIENFNK

Target ORF information:

RefSeq Version	NM_137499.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster guanine nucleotide exchange factor in mesoderm, transcript variant G (GEFmeso), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137499.3

ORF Insert Sequence:

ATGGAGCAGC TGGCGGCCAC CAGTGCGCAA CTGTTGCATG CCAAGCCTGC CACGCCCGGA 
ACTCCGGCCA CGCCCACTCC CAGTGCAACC AAAGCCAAGG AGTTTGTGTG CAGTGAGCCA 
CTGAGTCCCG TGGATATAGT GGACACCATT AACTTCGATC TGGTTGCGCA GCATATGGAA 
AGATTGACTC TGGCGGATCC AGGAGATTTG GATCCCAGGG CGGAGCTAAT AGAAGCGGTT 
AACAAAACAC TGGTTAACAA GCCACTGGTT AGGAGCTCCT CCGCCGCCTG TGCCTCCACA 
ATATGCAGTT CACCGCTGTT GACCTATGCC CGCACCAAGA GTTTGGGCGC CAAGGCCAGC 
ACTTTGGGCG GTGGAGTGCC TATTAAGCTG CCCACTGCCG AACAGCTGGC CGAGCTGGAG 
GAGGCCAACA AGTTGTCGGC GGAAAGTCTG GACAGGCTCA CCGATATCAA GCCCAAGTTT 
GCAGCTCGCC TAAGTGAGCT GGCTAGGCTG AATAACCGCC CACTGTCCTC CTCCTCCATT 
TGCTCCACCT CCTCCAGTTC CAGTTCCGGT TCGGACCAAC TGCTCAATGG AAAACTGATG 
GCCACTTCCT ACTTGGCTAG CGTAGAAAGT CTGGCGGAGA GTGAGAACGA GCTGGGCGAT 
CAACACCATC CGCCGGCGAT GAGTGTCCTC GAGAAAACCT GCCTAGAGAT CGTAGACTCG 
GAGCGAAGCT TCGTCGAGGA TTTGGGTCAG GTCATCAAGG GATACCTGCA GGACTGGAAG 
GAGCGTGCGT GCCTGCGCGT AGACGAATTG CAAATACTCT TCGCCAACAT TGAAGAGATT 
TATGAATTCA ACTCAATGCT TCTGCAGCGA CTTATCAACA CGGGAAGGGA TCCCGGGAGG 
ATCGCCAGGT GCTTCATTGA CCTGCGCGAT GGCTTTGATG TGTACACAAC CTACTGCACA 
AGCTACCCGG AAGCCATATC GTTGCTGACC AAGTTGCTGC AGGCCACGCA CACGTACTCT 
CTGCTTGCCT CCACGCAGAA GCTACTCCAA CATCGCCTGC CCCTGGGATC CTACCTCCTG 
AAACCGGTGC AGCGCATCCT CAAGTACCAT CTGCTGCTGG ACAGCCTGCG CAAGCACTGC 
GATGTGAAGG AGGTGGTCGA GGCCCATGTG ATCATGCGCC AGGTGGCGCA CAACATTGAC 
CAGGTGAAGC GAAAGCAGGA GCAGCAGAGT CGCGTCAAGG AGCTGTCCGG CATTCTGGAC 
GGCTGGCTGG GGCCAGAGCT CACTGTTCTG GGAGAGTTGC GCCAAGAGGG CCTGCTGATG 
GAGCAGCACA ATAAACAGCG CCTGGTACTG CTATTCGCCA CCATGCTGAT CATCACCAAG 
CAAAAGGAGG ACGGACGCCT TCAGTTCAAG ACTTACATTT CGCAAAACAA TCTGATGCTG 
AGCGAACACT TGCCCGGTGA ACCGACCAGC TTCTATGTCA TCCCCTTCGA CGAGCCGCGC 
CATCAAATCA AACTGACGGC TCGGAATCGT GACCAGAAGC GCATCTGGAC GCAGCACATC 
AAAGGTGTCA TGCTGGAGAA GTTAGACATT CCGATGCGCG CCAAGGAGCT GGTCTATCAG 
CTGGGCAACG AGGAAGATCG CACGCCAGAT CGCAGCACAT GGAAGTGGAG CCTTCACTCG 
GGAAGTAACT CGACTCCAAC TTACCTGGAG CGAAGGAACG CCTGCCGGAG GAGTGAGATA 
CGCCAGCGGA ATTCAAAGTT CAAGCGGAAG ACGGTGGCCA ACTCCAGTTC CTTTGACAGT 
TTCAATGAGT CCTTGGAACA GGAGGAGGAA TCGATTAGCC AGGCGAAGCC CTCGAAGCCA 
CTGGCCAGAA ATAACAGCCT AGACGATACT GCTCTACAGA AACTTGCTGC TGCTATGAGA 
TATCGCAAAA GAGATGCTGC AGTGAAGGAG GAGACGAAGA CGCCGGTGAA GGAGCCTTGT 
AAGTGCGACC AGGATGCGAA ACAGGACGAG CGGATTGAAC CGTGTTCCTG CATTCTGCGG 
GATCAGCGCA GTCGCAGTGC CAAGTCACAA TCTCCTGTCT TCAGCTACAA GGCACTCAAG 
GAGCGCAGCA AGTCGGTTCC CCGGATCGGA GGCTTTAGTT TGGACGAGGA GGCTGAGAGA 
GAACGGGAAC AGGACGAGGA TAGCGCAGCC TCTCTGAGGG GCAGCAAGCC CGATCTTAGC 
GAGCGCAAGA CCAAGGGAAA GGGATTCTTT GAAGTTAAGC AATACAACCA CAAGACGATG 
CCCAAAAAGA TAGCCAATTT GAAGAAGCAA CGAAGTAGCA CCACCAAGAG CTGCTCCAGG 
TTCTACATGG ATCTCAGCGA ATTCGATAGC AGCAGTTCGA CTGTACTCAA GATAACCGAA 
TCCACCGAGG AGCTGCGTCC AGATGGGGAA ATCGAACTGG CCCAAGAAGC CGCCACTCTG 
GATGAGTCCA CGCAGGATCA GTATTACAGC CCAGATCACG AGACGGAACT ACTGTCCCCA 
GCAGAGAAGT CAAAGCGAGA TGCACAGATT GTCCTTGATT TGCTAAAGAA TAGCAAGGAA 
TTTGAGAGGA TCTACAACAA GCAACAGAAG AGGCGCGAAA GTCCAGTGAG TCCCGTGAGT 
CCCGATATAA GTCGTGGTCC CATTCTGCCG CCTCCGCGTC CACCATCGAG ATCTCCACCA 
CCCTTGGAAC TGGAGGAGCA GGAGAAGAAA CCAGTCGAAG AGGTGGATTC AGTCAGCGAG 
GAGCCCATTT ACGAGACTCT GCTGCGCAAT GTCCATGTGC CCTACAAATT CTCACCGGTG 
TTGGGCCGAG CCAAGTCCTC GCAGATACTG AAGAAGCGAT CAAAGACTGC TCCCGCAGCC 
TCACGACCGG AATCGGATTA CGTGACCCTA GTCTACTCGC CCGAAGGAGT TCTGCAGCGA 
GTGGGCGAGG AGGTGGTGCA GAGGGATAGC TGCAGTTCCT CCACCACTTC GACATCCTCA 
AATGGATCGG GGTCCACGCT GAAACGAGAC AGCCAGAGCA CAGTGATCAA TCTTTTGATG 
GAGGCGGCGC AAGAGCACGA ATCACGTCCC TTGCCGAAGA ATTCAGTTTC AGGATCGGAG 
AGTTCTCTGC TGCCACGGGC TCTGAAGTCC ATAGACAATC TGAGCATGGC CTTCGGACGC 
TCCAACCGAC CGCCGGAGCG CCGGGTGTCC GATGTGAGCG AGATGTGCCG TCAGAGTGTG 
CTTCATCGCC AGGGCAGCGA GGCGGTGGGT GAGAGAATGG CCCATGTGGA TTACGCTGAT 
CCCAAGGCTC TATTTGGACT GGGGGTCCTG AACCACTCGG AACGTGACTC GGTCTTCTCG 
CTGACCTCCT CCTCCAGTGA CAGCATGTGC GAGCAGCAGC GCAAGCGATC CGGCGTGGGC 
GCCACCTTCG AGTATGAGCA GCAGGTGGAG GATAGCTTGG AGAATGATTT TCGCGACTCG 
GCCATTTACA GCGATGACAA CGAGAAGCGT TCCGGGGACT ACGATGTCCA TCTACGCCGT 
CCCAGCAGTC CGCCACCACC ACCGCCTCCG CTGGGGCCGC GTCCCCATCA GCCACCACAG 
ATTGCGCCGA AGCCTTCCAA GGATCAGCTA ATCCAGCAGC GATCGGGCGT CATCGTCTGC 
CAGTCGGCCT CCCGCAGCTG GGTGCTTCAG CAGATTGAGA ACTTCAACAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GEFmeso ( NM_176223.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_788403.2 Drosophila melanogaster guanine nucleotide exchange factor in mesoderm, transcript variant E (GEFmeso), mRNA.

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CloneID	OFa10503
Clone ID Related Accession (Same CDS sequence)	NM_176223.4
Accession Version	NM_176223.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4650bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	guanine nucleotide exchange factor in mesoderm, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_176223.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACATAG AATCCGATTC GGACACCTCG GAGAGATGGG AGGACTTTTT TGGCAGCTCC 
ACCGAATTGG CACCCTCGCT GCTGGGCATC TACGACACAT TGACAAACAG CTTTGCCGCG 
GGGGGCGTGG CAGCTGCTCC CACTGCGACA TCTCACGAAT CGAAGGCATC CAGCAGCGGC 
GACTCGACGC CCAAGAGGAG CAACAGTAAC TGCAGCAGCA GCAGCAGCAC TGGCTCATCG 
ACTGGCAGCT GTTCGAGTGT CCCCACCAGC GATGACCAGT CTGCTAGCTA CACGCTAGCC 
AGCTCCAGCA CTCATTTGGG CCACCAGGAG CCGCCCGCTC CACTGGTGGT TGCGGGCCTG 
CGGGACGAAC CGGATGGCGC TCAGTTGAGC AGGTTGGTGC AGCGCAGAAG CCAGGAGTCG 
CAGATTCACG GCAGCAGCAG CAGCATAGCC TCGAGTGGGA GCAGTCGACG GGGCAGCAAC 
ATCCGCATCC TTTCGCCCAA TGTTCATAGA ATCATCACGC ACTCGGATAT TCAACCAGTG 
GAGGCGGTGT CCGCGGAGGC ACTGAAACAG CATCAGCAGC AATCCCAGAG GAGTCCCACC 
CAGGGCAGGT ATATAAAGAC CGGTCATAGC GGAACACCAC CAGCCGCTGG TTCCGCACCC 
AAGCTGAAGC TCTCGCACAT ACCGCTGTCC AAGATCACCG GCAAGTTGTC CACCCAGTCT 
GGCAGCGAAC TGGTGTCGAC CTTCGATTCC AGCAACGAGT ATTTGAACTC GGTGGCCTTC 
GCCGCTTTCG AGAAATCCAA GCCGCAGGAC GAGGATCGGA CGCCAACGAA CAAATCGGTA 
CCAGGCACTC CCTTCCATTT CGATGGGCAT CCGTTTCAGG GGCGAAAACT AACCCTACCA 
CGGTACGATT CACAGCAGAG CATCAAGCTG ATCGAAATGG AGCAGCTGGC GGCCACCAGT 
GCGCAACTGT TGCATGCCAA GCCTGCCACG CCCGGAACTC CGGCCACGCC CACTCCCAGT 
GCAACCAAAG CCAAGGAGTT TGTGTGCAGT GAGCCACTGA GTCCCGTGGA TATAGTGGAC 
ACCATTAACT TCGATCTGGT TGCGCAGCAT ATGGAAAGAT TGACTCTGGC GGATCCAGGA 
GATTTGGATC CCAGGGCGGA GCTAATAGAA GCGGTTAACA AAACACTGGT TAACAAGCCA 
CTGGTTAGGA GCTCCTCCGC CGCCTGTGCC TCCACAATAT GCAGTTCACC GCTGTTGACC 
TATGCCCGCA CCAAGAGTTT GGGCGCCAAG GCCAGCACTT TGGGCGGTGG AGTGCCTATT 
AAGCTGCCCA CTGCCGAACA GCTGGCCGAG CTGGAGGAGG CCAACAAGTT GTCGGCGGAA 
AGTCTGGACA GGCTCACCGA TATCAAGCCC AAGTTTGCAG CTCGCCTAAG TGAGCTGGCT 
AGGCTGAATA ACCGCCCACT GTCCTCCTCC TCCATTTGCT CCACCTCCTC CAGTTCCAGT 
TCCGGTTCGG ACCAACTGCT CAATGGAAAA CTGATGGCCA CTTCCTACTT GGCTAGCGTA 
GAAAGTCTGG CGGAGAGTGA GAACGAGCTG GGCGATCAAC ACCATCCGCC GGCGATGAGT 
GTCCTCGAGA AAACCTGCCT AGAGATCGTA GACTCGGAGC GAAGCTTCGT CGAGGATTTG 
GGTCAGGTCA TCAAGGGATA CCTGCAGGAC TGGAAGGAGC GTGCGTGCCT GCGCGTAGAC 
GAATTGCAAA TACTCTTCGC CAACATTGAA GAGATTTATG AATTCAACTC AATGCTTCTG 
CAGCGACTTA TCAACACGGG AAGGGATCCC GGGAGGATCG CCAGGTGCTT CATTGACCTG 
CGCGATGGCT TTGATGTGTA CACAACCTAC TGCACAAGCT ACCCGGAAGC CATATCGTTG 
CTGACCAAGT TGCTGCAGGC CACGCACACG TACTCTCTGC TTGCCTCCAC GCAGAAGCTA 
CTCCAACATC GCCTGCCCCT GGGATCCTAC CTCCTGAAAC CGGTGCAGCG CATCCTCAAG 
TACCATCTGC TGCTGGACAG CCTGCGCAAG CACTGCGATG TGAAGGAGGT GGTCGAGGCC 
CATGTGATCA TGCGCCAGGT GGCGCACAAC ATTGACCAGG TGAAGCGAAA GCAGGAGCAG 
CAGAGTCGCG TCAAGGAGCT GTCCGGCATT CTGGACGGCT GGCTGGGGCC AGAGCTCACT 
GTTCTGGGAG AGTTGCGCCA AGAGGGCCTG CTGATGGAGC AGCACAATAA ACAGCGCCTG 
GTACTGCTAT TCGCCACCAT GCTGATCATC ACCAAGCAAA AGGAGGACGG ACGCCTTCAG 
TTCAAGACTT ACATTTCGCA AAACAATCTG ATGCTGAGCG AACACTTGCC CGGTGAACCG 
ACCAGCTTCT ATGTCATCCC CTTCGACGAG CCGCGCCATC AAATCAAACT GACGGCTCGG 
AATCGTGACC AGAAGCGCAT CTGGACGCAG CACATCAAAG GTGTCATGCT GGAGAAGTTA 
GACATTCCGA TGCGCGCCAA GGAGCTGGTC TATCAGCTGG GCAACGAGGA AGATCGCACG 
CCAGATCGCA GCACATGGAA GTGGAGCCTT CACTCGGGAA GTAACTCGAC TCCAACTTAC 
CTGGAGCGAA GGAACGCCTG CCGGAGGAGT GAGATACGCC AGCGGAATTC AAAGTTCAAG 
CGGAAGACGG TGGCCAACTC CAGTTCCTTT GACAGTTTCA ATGAGTCCTT GGAACAGGAG 
GAGGAATCGA TTAGCCAGGC GAAGCCCTCG AAGCCACTGG CCAGAAATAA CAGCCTAGAC 
GATACTGCTC TACAGAAACT TGCTGCTGCT ATGAGATATC GCAAAAGAGA TGCTGCAGTG 
AAGGAGGAGA CGAAGACGCC GGTGAAGGAG CCTTGTAAGT GCGACCAGGA TGCGAAACAG 
GACGAGCGGA TTGAACCGTG TTCCTGCATT CTGCGGGATC AGCGCAGTCG CAGTGCCAAG 
TCACAATCTC CTGTCTTCAG CTACAAGGCA CTCAAGGAGC GCAGCAAGTC GGTTCCCCGG 
ATCGGAGGCT TTAGTTTGGA CGAGGAGGCT GAGAGAGAAC GGGAACAGGA CGAGGATAGC 
GCAGCCTCTC TGAGGGGCAG CAAGCCCGAT CTTAGCGAGC GCAAGACCAA GGGAAAGGGA 
TTCTTTGAAG TTAAGCAATA CAACCACAAG ACGATGCCCA AAAAGATAGC CAATTTGAAG 
AAGCAACGAA GTAGCACCAC CAAGAGCTGC TCCAGGTTCT ACATGGATCT CAGCGAATTC 
GATAGCAGCA GTTCGACTGT ACTCAAGATA ACCGAATCCA CCGAGGAGCT GCGTCCAGAT 
GGGGAAATCG AACTGGCCCA AGAAGCCGCC ACTCTGGATG AGTCCACGCA GGATCAGTAT 
TACAGCCCAG ATCACGAGAC GGAACTACTG TCCCCAGCAG AGAAGTCAAA GCGAGATGCA 
CAGATTGTCC TTGATTTGCT AAAGAATAGC AAGGAATTTG AGAGGATCTA CAACAAGCAA 
CAGAAGAGGC GCGAAAGTCC AGTGAGTCCC GTGAGTCCCG ATATAAGTCG TGGTCCCATT 
CTGCCGCCTC CGCGTCCACC ATCGAGATCT CCACCACCCT TGGAACTGGA GGAGCAGGAG 
AAGAAACCAG TCGAAGAGGT GGATTCAGTC AGCGAGGAGC CCATTTACGA GACTCTGCTG 
CGCAATGTCC ATGTGCCCTA CAAATTCTCA CCGGTGTTGG GCCGAGCCAA GTCCTCGCAG 
ATACTGAAGA AGCGATCAAA GACTGCTCCC GCAGCCTCAC GACCGGAATC GGATTACGTG 
ACCCTAGTCT ACTCGCCCGA AGGAGTTCTG CAGCGAGTGG GCGAGGAGGT GGTGCAGAGG 
GATAGCTGCA GTTCCTCCAC CACTTCGACA TCCTCAAATG GATCGGGGTC CACGCTGAAA 
CGAGACAGCC AGAGCACAGT GATCAATCTT TTGATGGAGG CGGCGCAAGA GCACGAATCA 
CGTCCCTTGC CGAAGAATTC AGTTTCAGGA TCGGAGAGTT CTCTGCTGCC ACGGGCTCTG 
AAGTCCATAG ACAATCTGAG CATGGCCTTC GGACGCTCCA ACCGACCGCC GGAGCGCCGG 
GTGTCCGATG TGAGCGAGAT GTGCCGTCAG AGTGTGCTTC ATCGCCAGGG CAGCGAGGCG 
GTGGGTGAGA GAATGGCCCA TGTGGATTAC GCTGATCCCA AGGCTCTATT TGGACTGGGG 
GTCCTGAACC ACTCGGAACG TGACTCGGTC TTCTCGCTGA CCTCCTCCTC CAGTGACAGC 
ATGTGCGAGC AGCAGCGCAA GCGATCCGGC GTGGGCGCCA CCTTCGAGTA TGAGCAGCAG 
GTGGAGGATA GCTTGGAGAA TGATTTTCGC GACTCGGCCA TTTACAGCGA TGACAACGAG 
AAGCGTTCCG GGGACTACGA TGTCCATCTA CGCCGTCCCA GCAGTCCGCC ACCACCACCG 
CCTCCGCTGG GGCCGCGTCC CCATCAGCCA CCACAGATTG CGCCGAAGCC TTCCAAGGAT 
CAGCTAATCC AGCAGCGATC GGGCGTCATC GTCTGCCAGT CGGCCTCCCG CAGCTGGGTG 
CTTCAGCAGA TTGAGAACTT CAACAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788403.2
CDS	1046..5695
Translation	MDIESDSDTSERWEDFFGSSTELAPSLLGIYDTLTNSFAAGGVAAAPTATSHESKASSSGDSTPKRSNSNCSSSSSTGSSTGSCSSVPTSDDQSASYTLASSSTHLGHQEPPAPLVVAGLRDEPDGAQLSRLVQRRSQESQIHGSSSSIASSGSSRRGSNIRILSPNVHRIITHSDIQPVEAVSAEALKQHQQQSQRSPTQGRYIKTGHSGTPPAAGSAPKLKLSHIPLSKITGKLSTQSGSELVSTFDSSNEYLNSVAFAAFEKSKPQDEDRTPTNKSVPGTPFHFDGHPFQGRKLTLPRYDSQQSIKLIEMEQLAATSAQLLHAKPATPGTPATPTPSATKAKEFVCSEPLSPVDIVDTINFDLVAQHMERLTLADPGDLDPRAELIEAVNKTLVNKPLVRSSSAACASTICSSPLLTYARTKSLGAKASTLGGGVPIKLPTAEQLAELEEANKLSAESLDRLTDIKPKFAARLSELARLNNRPLSSSSICSTSSSSSSGSDQLLNGKLMATSYLASVESLAESENELGDQHHPPAMSVLEKTCLEIVDSERSFVEDLGQVIKGYLQDWKERACLRVDELQILFANIEEIYEFNSMLLQRLINTGRDPGRIARCFIDLRDGFDVYTTYCTSYPEAISLLTKLLQATHTYSLLASTQKLLQHRLPLGSYLLKPVQRILKYHLLLDSLRKHCDVKEVVEAHVIMRQVAHNIDQVKRKQEQQSRVKELSGILDGWLGPELTVLGELRQEGLLMEQHNKQRLVLLFATMLIITKQKEDGRLQFKTYISQNNLMLSEHLPGEPTSFYVIPFDEPRHQIKLTARNRDQKRIWTQHIKGVMLEKLDIPMRAKELVYQLGNEEDRTPDRSTWKWSLHSGSNSTPTYLERRNACRRSEIRQRNSKFKRKTVANSSSFDSFNESLEQEEESISQAKPSKPLARNNSLDDTALQKLAAAMRYRKRDAAVKEETKTPVKEPCKCDQDAKQDERIEPCSCILRDQRSRSAKSQSPVFSYKALKERSKSVPRIGGFSLDEEAEREREQDEDSAASLRGSKPDLSERKTKGKGFFEVKQYNHKTMPKKIANLKKQRSSTTKSCSRFYMDLSEFDSSSSTVLKITESTEELRPDGEIELAQEAATLDESTQDQYYSPDHETELLSPAEKSKRDAQIVLDLLKNSKEFERIYNKQQKRRESPVSPVSPDISRGPILPPPRPPSRSPPPLELEEQEKKPVEEVDSVSEEPIYETLLRNVHVPYKFSPVLGRAKSSQILKKRSKTAPAASRPESDYVTLVYSPEGVLQRVGEEVVQRDSCSSSTTSTSSNGSGSTLKRDSQSTVINLLMEAAQEHESRPLPKNSVSGSESSLLPRALKSIDNLSMAFGRSNRPPERRVSDVSEMCRQSVLHRQGSEAVGERMAHVDYADPKALFGLGVLNHSERDSVFSLTSSSSDSMCEQQRKRSGVGATFEYEQQVEDSLENDFRDSAIYSDDNEKRSGDYDVHLRRPSSPPPPPPPLGPRPHQPPQIAPKPSKDQLIQQRSGVIVCQSASRSWVLQQIENFNK

Target ORF information:

RefSeq Version	NM_176223.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster guanine nucleotide exchange factor in mesoderm, transcript variant E (GEFmeso), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176223.4

ORF Insert Sequence:

ATGGACATAG AATCCGATTC GGACACCTCG GAGAGATGGG AGGACTTTTT TGGCAGCTCC 
ACCGAATTGG CACCCTCGCT GCTGGGCATC TACGACACAT TGACAAACAG CTTTGCCGCG 
GGGGGCGTGG CAGCTGCTCC CACTGCGACA TCTCACGAAT CGAAGGCATC CAGCAGCGGC 
GACTCGACGC CCAAGAGGAG CAACAGTAAC TGCAGCAGCA GCAGCAGCAC TGGCTCATCG 
ACTGGCAGCT GTTCGAGTGT CCCCACCAGC GATGACCAGT CTGCTAGCTA CACGCTAGCC 
AGCTCCAGCA CTCATTTGGG CCACCAGGAG CCGCCCGCTC CACTGGTGGT TGCGGGCCTG 
CGGGACGAAC CGGATGGCGC TCAGTTGAGC AGGTTGGTGC AGCGCAGAAG CCAGGAGTCG 
CAGATTCACG GCAGCAGCAG CAGCATAGCC TCGAGTGGGA GCAGTCGACG GGGCAGCAAC 
ATCCGCATCC TTTCGCCCAA TGTTCATAGA ATCATCACGC ACTCGGATAT TCAACCAGTG 
GAGGCGGTGT CCGCGGAGGC ACTGAAACAG CATCAGCAGC AATCCCAGAG GAGTCCCACC 
CAGGGCAGGT ATATAAAGAC CGGTCATAGC GGAACACCAC CAGCCGCTGG TTCCGCACCC 
AAGCTGAAGC TCTCGCACAT ACCGCTGTCC AAGATCACCG GCAAGTTGTC CACCCAGTCT 
GGCAGCGAAC TGGTGTCGAC CTTCGATTCC AGCAACGAGT ATTTGAACTC GGTGGCCTTC 
GCCGCTTTCG AGAAATCCAA GCCGCAGGAC GAGGATCGGA CGCCAACGAA CAAATCGGTA 
CCAGGCACTC CCTTCCATTT CGATGGGCAT CCGTTTCAGG GGCGAAAACT AACCCTACCA 
CGGTACGATT CACAGCAGAG CATCAAGCTG ATCGAAATGG AGCAGCTGGC GGCCACCAGT 
GCGCAACTGT TGCATGCCAA GCCTGCCACG CCCGGAACTC CGGCCACGCC CACTCCCAGT 
GCAACCAAAG CCAAGGAGTT TGTGTGCAGT GAGCCACTGA GTCCCGTGGA TATAGTGGAC 
ACCATTAACT TCGATCTGGT TGCGCAGCAT ATGGAAAGAT TGACTCTGGC GGATCCAGGA 
GATTTGGATC CCAGGGCGGA GCTAATAGAA GCGGTTAACA AAACACTGGT TAACAAGCCA 
CTGGTTAGGA GCTCCTCCGC CGCCTGTGCC TCCACAATAT GCAGTTCACC GCTGTTGACC 
TATGCCCGCA CCAAGAGTTT GGGCGCCAAG GCCAGCACTT TGGGCGGTGG AGTGCCTATT 
AAGCTGCCCA CTGCCGAACA GCTGGCCGAG CTGGAGGAGG CCAACAAGTT GTCGGCGGAA 
AGTCTGGACA GGCTCACCGA TATCAAGCCC AAGTTTGCAG CTCGCCTAAG TGAGCTGGCT 
AGGCTGAATA ACCGCCCACT GTCCTCCTCC TCCATTTGCT CCACCTCCTC CAGTTCCAGT 
TCCGGTTCGG ACCAACTGCT CAATGGAAAA CTGATGGCCA CTTCCTACTT GGCTAGCGTA 
GAAAGTCTGG CGGAGAGTGA GAACGAGCTG GGCGATCAAC ACCATCCGCC GGCGATGAGT 
GTCCTCGAGA AAACCTGCCT AGAGATCGTA GACTCGGAGC GAAGCTTCGT CGAGGATTTG 
GGTCAGGTCA TCAAGGGATA CCTGCAGGAC TGGAAGGAGC GTGCGTGCCT GCGCGTAGAC 
GAATTGCAAA TACTCTTCGC CAACATTGAA GAGATTTATG AATTCAACTC AATGCTTCTG 
CAGCGACTTA TCAACACGGG AAGGGATCCC GGGAGGATCG CCAGGTGCTT CATTGACCTG 
CGCGATGGCT TTGATGTGTA CACAACCTAC TGCACAAGCT ACCCGGAAGC CATATCGTTG 
CTGACCAAGT TGCTGCAGGC CACGCACACG TACTCTCTGC TTGCCTCCAC GCAGAAGCTA 
CTCCAACATC GCCTGCCCCT GGGATCCTAC CTCCTGAAAC CGGTGCAGCG CATCCTCAAG 
TACCATCTGC TGCTGGACAG CCTGCGCAAG CACTGCGATG TGAAGGAGGT GGTCGAGGCC 
CATGTGATCA TGCGCCAGGT GGCGCACAAC ATTGACCAGG TGAAGCGAAA GCAGGAGCAG 
CAGAGTCGCG TCAAGGAGCT GTCCGGCATT CTGGACGGCT GGCTGGGGCC AGAGCTCACT 
GTTCTGGGAG AGTTGCGCCA AGAGGGCCTG CTGATGGAGC AGCACAATAA ACAGCGCCTG 
GTACTGCTAT TCGCCACCAT GCTGATCATC ACCAAGCAAA AGGAGGACGG ACGCCTTCAG 
TTCAAGACTT ACATTTCGCA AAACAATCTG ATGCTGAGCG AACACTTGCC CGGTGAACCG 
ACCAGCTTCT ATGTCATCCC CTTCGACGAG CCGCGCCATC AAATCAAACT GACGGCTCGG 
AATCGTGACC AGAAGCGCAT CTGGACGCAG CACATCAAAG GTGTCATGCT GGAGAAGTTA 
GACATTCCGA TGCGCGCCAA GGAGCTGGTC TATCAGCTGG GCAACGAGGA AGATCGCACG 
CCAGATCGCA GCACATGGAA GTGGAGCCTT CACTCGGGAA GTAACTCGAC TCCAACTTAC 
CTGGAGCGAA GGAACGCCTG CCGGAGGAGT GAGATACGCC AGCGGAATTC AAAGTTCAAG 
CGGAAGACGG TGGCCAACTC CAGTTCCTTT GACAGTTTCA ATGAGTCCTT GGAACAGGAG 
GAGGAATCGA TTAGCCAGGC GAAGCCCTCG AAGCCACTGG CCAGAAATAA CAGCCTAGAC 
GATACTGCTC TACAGAAACT TGCTGCTGCT ATGAGATATC GCAAAAGAGA TGCTGCAGTG 
AAGGAGGAGA CGAAGACGCC GGTGAAGGAG CCTTGTAAGT GCGACCAGGA TGCGAAACAG 
GACGAGCGGA TTGAACCGTG TTCCTGCATT CTGCGGGATC AGCGCAGTCG CAGTGCCAAG 
TCACAATCTC CTGTCTTCAG CTACAAGGCA CTCAAGGAGC GCAGCAAGTC GGTTCCCCGG 
ATCGGAGGCT TTAGTTTGGA CGAGGAGGCT GAGAGAGAAC GGGAACAGGA CGAGGATAGC 
GCAGCCTCTC TGAGGGGCAG CAAGCCCGAT CTTAGCGAGC GCAAGACCAA GGGAAAGGGA 
TTCTTTGAAG TTAAGCAATA CAACCACAAG ACGATGCCCA AAAAGATAGC CAATTTGAAG 
AAGCAACGAA GTAGCACCAC CAAGAGCTGC TCCAGGTTCT ACATGGATCT CAGCGAATTC 
GATAGCAGCA GTTCGACTGT ACTCAAGATA ACCGAATCCA CCGAGGAGCT GCGTCCAGAT 
GGGGAAATCG AACTGGCCCA AGAAGCCGCC ACTCTGGATG AGTCCACGCA GGATCAGTAT 
TACAGCCCAG ATCACGAGAC GGAACTACTG TCCCCAGCAG AGAAGTCAAA GCGAGATGCA 
CAGATTGTCC TTGATTTGCT AAAGAATAGC AAGGAATTTG AGAGGATCTA CAACAAGCAA 
CAGAAGAGGC GCGAAAGTCC AGTGAGTCCC GTGAGTCCCG ATATAAGTCG TGGTCCCATT 
CTGCCGCCTC CGCGTCCACC ATCGAGATCT CCACCACCCT TGGAACTGGA GGAGCAGGAG 
AAGAAACCAG TCGAAGAGGT GGATTCAGTC AGCGAGGAGC CCATTTACGA GACTCTGCTG 
CGCAATGTCC ATGTGCCCTA CAAATTCTCA CCGGTGTTGG GCCGAGCCAA GTCCTCGCAG 
ATACTGAAGA AGCGATCAAA GACTGCTCCC GCAGCCTCAC GACCGGAATC GGATTACGTG 
ACCCTAGTCT ACTCGCCCGA AGGAGTTCTG CAGCGAGTGG GCGAGGAGGT GGTGCAGAGG 
GATAGCTGCA GTTCCTCCAC CACTTCGACA TCCTCAAATG GATCGGGGTC CACGCTGAAA 
CGAGACAGCC AGAGCACAGT GATCAATCTT TTGATGGAGG CGGCGCAAGA GCACGAATCA 
CGTCCCTTGC CGAAGAATTC AGTTTCAGGA TCGGAGAGTT CTCTGCTGCC ACGGGCTCTG 
AAGTCCATAG ACAATCTGAG CATGGCCTTC GGACGCTCCA ACCGACCGCC GGAGCGCCGG 
GTGTCCGATG TGAGCGAGAT GTGCCGTCAG AGTGTGCTTC ATCGCCAGGG CAGCGAGGCG 
GTGGGTGAGA GAATGGCCCA TGTGGATTAC GCTGATCCCA AGGCTCTATT TGGACTGGGG 
GTCCTGAACC ACTCGGAACG TGACTCGGTC TTCTCGCTGA CCTCCTCCTC CAGTGACAGC 
ATGTGCGAGC AGCAGCGCAA GCGATCCGGC GTGGGCGCCA CCTTCGAGTA TGAGCAGCAG 
GTGGAGGATA GCTTGGAGAA TGATTTTCGC GACTCGGCCA TTTACAGCGA TGACAACGAG 
AAGCGTTCCG GGGACTACGA TGTCCATCTA CGCCGTCCCA GCAGTCCGCC ACCACCACCG 
CCTCCGCTGG GGCCGCGTCC CCATCAGCCA CCACAGATTG CGCCGAAGCC TTCCAAGGAT 
CAGCTAATCC AGCAGCGATC GGGCGTCATC GTCTGCCAGT CGGCCTCCCG CAGCTGGGTG 
CTTCAGCAGA TTGAGAACTT CAACAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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GEFmeso cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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