Ote cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ote
Entrez Gene ID	37090
Full Name	Otefin
Synonyms	CG5581,Dmel\CG5581,hal,ote,otefin
General protein information	Preferred Names Otefin Names CG5581 gene product from transcript CG5581-RA CG5581-PA Ote-PA halted haulted otefin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 55A1-55A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_057316.5	NP_476664.2	otefin

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ote is required autonomously for survival of male and female stem cell populations, as well as non-autonomously for maintenance of two somatic niches. Ote loss in ovary causes a decrease in cap cell number and altered signal transduction. Ote mutant males become prematurely sterile as they age.
Title: Drosophila male and female germline stem cell niches require the nuclear lamina protein Otefin.

production of Bocks, but not dMAN1, rescued ote-/- sterility, demonstrating that Ote and Bocks are functional homologs
Title: Unique and shared functions of nuclear lamina LEM domain proteins in Drosophila.

We show that the primary defect in ote(-/-) GSCs is a block of differentiation, which ultimately leads to germ cell death.
Title: The Drosophila nuclear lamina protein otefin is required for germline stem cell survival.

Phosphorylation of Ote at T63 is critical for mitotic progression.
Title: Functional analysis of centrosomal kinase substrates in Drosophila melanogaster reveals a new function of the nuclear envelope component otefin in cell cycle progression.

Data show that otefin is essential for germline stem cell (GSC) maintenance, is required for the Dpp/BMP signaling pathway to silence bam transcription, and that Ote physically interacts with Medea/Smad4 at the bam silencer element to regulate GSC fate.
Title: Otefin, a nuclear membrane protein, determines the fate of germline stem cells in Drosophila via interaction with Smad complexes.

The following Ote gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ote cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa10454	NM_057316.5 Latest version!	Drosophila melanogaster otefin (Ote), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ote ( NM_057316.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476664.2 Drosophila melanogaster otefin (Ote), mRNA.

Price & Availability↑

CloneID	OFa10454
Clone ID Related Accession (Same CDS sequence)	NM_057316.5
Accession Version	NM_057316.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	otefin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_057316.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGATG TGGACGATTT TGATTCTCTG TCCAATGCTG AGTTGCGCGC CAAGATGCTG 
GCACAGGGGC TGCCCAACAT CCCCGTGACG GATAGCAGCC GGAAGGTTCT AGTAAAGCGT 
CTGCGCGCCT CCATTGGTGG CCAGGCGTCG CCGGCCGCTA GTCCCAAGAA GACCAACAGG 
CGGGAGACCC TGGCTCCCGC ACCTGGTGCA CCATCTGCTC CGGCGGCAGC CTCCACGCCG 
GTGGACAAGC TGGATGGCAA CAAGGTGGCG CCCGCCACAA AGGCTCGGCG CACAATTACC 
GCTGCAGAGG CCAAGGAACC AGTGCGTCGA TTACCCGAGG AAGCTATACG CCGACGCCCC 
GACGAAGCCG ACCGCCTACG ATCCGAGGAG CCCGTTGCCG CCAGGAAGCC AACAACTGCT 
CCTGCTGCTC AACCCGTACA GACCCGGCGC ACTTCCACCT CCTCGGGGTC TGAAAGGAAA 
GTAGTGGAGC CCTTGAGGAA GCCAGAAACC ATTGTTGAGC AACCGGCTTC CTCGAAGCGC 
GCTGATCGAG AGGAGAATTA TCTTAAGGTC AACTCCCTGA TTGTCCTAGA GTCCGATGAA 
GAAGAGGACG AGCAGCTAGT CCAGGCTGCA GATCTGGTGG AGCAGGAGCA CGCGGCCAGA 
CAGAAGACCA CGAAGCTCGC AAGCAGCGGC ACCACTACGT ACGAGTACAA AAGCAAGGTT 
GTGGAGCCTC CACGCCGACA GGTCTATGAA GCGACAGCAG CTCCCGTGCT GCCGCCATCT 
GTGCCATCTG CCAGAGCGCA GACCACCAGT TCCACCAGGT CCTATGACTA TGCGAGCAAC 
CCAGCACCCG GTCGCTATAG CAGTTTTGTG CGTACTGCAG CCCAAGGCTA TGTGACTGCA 
GAGGCTCCGC CGGTGGCCTC CTACTCATCC AGCTACAAGC GTACCTATGC TAACGAGCTG 
AGCGACGACA CTGATTCCAA GGAAGACCAG TACGAAAGCA CATTTGCCAG GAATCTAGCT 
CGTTTGCGAG CCGAACGGAT TGGAGATCGC ATCAGCCCGT ATTCCAGACG CACTCTGGCC 
AGTGGAAACG CGGGTAGCGG TTCTCTCGGC TATGAGCCGC GTGCCAGGCG CTCCTTGCGC 
CCGAATGATA ACAGCGTATC CGAGGCCTTT AACCGTTGGT TGAACAGCCT GGAGCAGAAG 
TATCACATCA AGTCGAAGCT GTTCATTGTA CTCCTCGTCC TTTTGTTAAT CGGCGTTTAT 
TACATATTCT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476664.2
CDS	99..1373
Translation	MADVDDFDSLSNAELRAKMLAQGLPNIPVTDSSRKVLVKRLRASIGGQASPAASPKKTNRRETLAPAPGAPSAPAAASTPVDKLDGNKVAPATKARRTITAAEAKEPVRRLPEEAIRRRPDEADRLRSEEPVAARKPTTAPAAQPVQTRRTSTSSGSERKVVEPLRKPETIVEQPASSKRADREENYLKVNSLIVLESDEEEDEQLVQAADLVEQEHAARQKTTKLASSGTTTYEYKSKVVEPPRRQVYEATAAPVLPPSVPSARAQTTSSTRSYDYASNPAPGRYSSFVRTAAQGYVTAEAPPVASYSSSYKRTYANELSDDTDSKEDQYESTFARNLARLRAERIGDRISPYSRRTLASGNAGSGSLGYEPRARRSLRPNDNSVSEAFNRWLNSLEQKYHIKSKLFIVLLVLLLIGVYYIFY

Target ORF information:

RefSeq Version	NM_057316.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster otefin (Ote), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057316.5

ORF Insert Sequence:

ATGGCCGATG TGGACGATTT TGATTCTCTG TCCAATGCTG AGTTGCGCGC CAAGATGCTG 
GCACAGGGGC TGCCCAACAT CCCCGTGACG GATAGCAGCC GGAAGGTTCT AGTAAAGCGT 
CTGCGCGCCT CCATTGGTGG CCAGGCGTCG CCGGCCGCTA GTCCCAAGAA GACCAACAGG 
CGGGAGACCC TGGCTCCCGC ACCTGGTGCA CCATCTGCTC CGGCGGCAGC CTCCACGCCG 
GTGGACAAGC TGGATGGCAA CAAGGTGGCG CCCGCCACAA AGGCTCGGCG CACAATTACC 
GCTGCAGAGG CCAAGGAACC AGTGCGTCGA TTACCCGAGG AAGCTATACG CCGACGCCCC 
GACGAAGCCG ACCGCCTACG ATCCGAGGAG CCCGTTGCCG CCAGGAAGCC AACAACTGCT 
CCTGCTGCTC AACCCGTACA GACCCGGCGC ACTTCCACCT CCTCGGGGTC TGAAAGGAAA 
GTAGTGGAGC CCTTGAGGAA GCCAGAAACC ATTGTTGAGC AACCGGCTTC CTCGAAGCGC 
GCTGATCGAG AGGAGAATTA TCTTAAGGTC AACTCCCTGA TTGTCCTAGA GTCCGATGAA 
GAAGAGGACG AGCAGCTAGT CCAGGCTGCA GATCTGGTGG AGCAGGAGCA CGCGGCCAGA 
CAGAAGACCA CGAAGCTCGC AAGCAGCGGC ACCACTACGT ACGAGTACAA AAGCAAGGTT 
GTGGAGCCTC CACGCCGACA GGTCTATGAA GCGACAGCAG CTCCCGTGCT GCCGCCATCT 
GTGCCATCTG CCAGAGCGCA GACCACCAGT TCCACCAGGT CCTATGACTA TGCGAGCAAC 
CCAGCACCCG GTCGCTATAG CAGTTTTGTG CGTACTGCAG CCCAAGGCTA TGTGACTGCA 
GAGGCTCCGC CGGTGGCCTC CTACTCATCC AGCTACAAGC GTACCTATGC TAACGAGCTG 
AGCGACGACA CTGATTCCAA GGAAGACCAG TACGAAAGCA CATTTGCCAG GAATCTAGCT 
CGTTTGCGAG CCGAACGGAT TGGAGATCGC ATCAGCCCGT ATTCCAGACG CACTCTGGCC 
AGTGGAAACG CGGGTAGCGG TTCTCTCGGC TATGAGCCGC GTGCCAGGCG CTCCTTGCGC 
CCGAATGATA ACAGCGTATC CGAGGCCTTT AACCGTTGGT TGAACAGCCT GGAGCAGAAG 
TATCACATCA AGTCGAAGCT GTTCATTGTA CTCCTCGTCC TTTTGTTAAT CGGCGTTTAT 
TACATATTCT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Ote cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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