fj cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	fj
Entrez Gene ID	37089
Full Name	four-jointed
Synonyms	CG10917,Dmel\CG10917,Fj
General protein information	Preferred Names four-jointed Names CG10917 gene product from transcript CG10917-RA CG10917-PA fj-PA four jointed
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 55C1-55C1\|2-81.5 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_058135.4	NP_477483.1	four-jointed

Pathways from BioSystems

Homologies

Homo sapiens (human)	FJX1	NP_055159.2
Canis lupus familiaris (dog)	FJX1	XP_540543.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008093	XP_001237917.2
Pan troglodytes (chimpanzee)	FJX1	XP_508371.2
Bos taurus (cattle)	FJX1	XP_005216457.1
Danio rerio (zebrafish)	LOC100001423	XP_001337600.3
Drosophila melanogaster (fruit fly)	fj	NP_477483.1
Xenopus tropicalis (tropical clawed frog)	fjx1	XP_004913403.1
Mus musculus (house mouse)	Fjx1	NP_034348.2
Rattus norvegicus (Norway rat)	Fjx1	NP_001102425.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Four-jointed acts both on Fat to promote its binding to Dachsous and on Dachsous to inhibit its binding to Fat, with a bias towards a stronger effect on Fat
Title: Cellular interpretation of the long-range gradient of Four-jointed activity in the Drosophila wing.

The gradient of Fj expression, phosphorylation of Ft by Fj and sub-cellular distal accumulation of Ds are three critical elements required for generating sub-cellular asymmetry of Ft-Ds heterodimer.
Title: Mathematical modeling of sub-cellular asymmetry of fat-dachsous heterodimer for generation of planar cell polarity.

study shows how the Fat/Dachsous/Four-jointed planar cell polarity pathway controls morphogenesis
Title: Mechanical control of morphogenesis by Fat/Dachsous/Four-jointed planar cell polarity pathway.

Complementary expression of Ds and Fj results in biased Ft and Ds protein distribution across cells, with Ft and Ds accumulating on opposite edges. Asymmetries in protein distribution are transmitted to neighboring cells.
Title: Planar polarity specification through asymmetric subcellular localization of Fat and Dachsous.

effects on wing size and planar polarity by phosphorylating fat (ft) and dachsous (ds) activity
Title: Four-jointed modulates growth and planar polarity by reducing the affinity of dachsous for fat.

The following fj gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fj cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa10453	NM_058135.4 Latest version!	Drosophila melanogaster four-jointed (fj), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

fj ( NM_058135.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477483.1 Drosophila melanogaster four-jointed (fj), mRNA.

Price & Availability↑

CloneID	OFa10453
Clone ID Related Accession (Same CDS sequence)	NM_058135.4
Accession Version	NM_058135.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	four-jointed
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_058135.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTACGACA TCAAGCGCCT GGAAGCGGGA CAACAGAAAC TACAGCAAGC ACAGCAACCA 
CTGGGCCTTG ACCTGAGCGG CCAGCAACAG CAACTCACCT GCAGCGTGAT CACAGCGCCC 
GAGCATCGGG CTAATCCCAA TCCCAGCTCC ATTTCGCAAT CCAACCCCAG CGAGGCCACC 
CACATGACTT TGTTGACGCT GCGCCGGCGC CGTTCGCTGC AGCGACGCGC CTGCCTTCTG 
AGCATCCTCG CCGCCTTCGT CTTTGGCATG GCACTGGGCG TTGTTGTGCC CATGTTCGGC 
CTGCCCCGCC ACCAGGACTC CCCGCCAGAT CTGCCGGAGG AGCAGATACA GATGGTTGCC 
GTGGAGCCGC TGAGCAGCTA CCGCGTGGAG TTCATCAAGG AGACGGACGA GCTGAGTGCC 
GAGCAGGTAT TCCGCAATGC CTTCCATCTG GAGCAGGACA AGGATGCTCC GGACTCGATG 
GTGGTGAAGA AATTGGACAC CAACGACGGC AGCATCAAGG AATTCCATGT GCAGCGCACC 
GCCAGCGGTC GTTATCGCAA GGGTCCGGAG AGGAGGCTGT CCAAGAAGAT GCCGGAAAGG 
GTGCAGCCAC AAGAGACCTC TCGTTCTCCG ACGACCTCGC CGACAAATCC TACCAGTGAG 
CACCAAGCGG GATTTATTGA GGAGGACGTC TATTGGGGTC CCACTGTGGA GCAGGCTCTG 
CCCAAGGGAT TCGCCGCCAA GGACCAAGTC TCTTGGGAAC GCTTCGTTGG AGAGCAGGGC 
CGAGTAGTTC GCCTGGAGCA GGGATGCGGA AGAATGCAAA ATCGCATGGT GGTCTTTGCG 
GATGGAACAA GGGCCTGTGC CCGCTACCGC CAGAATACGG ATCAGATTCA GGGCGAGATA 
TTCAGCTACT ACTTGGGCCA GCTCTTAAAC ATAAGCAATC TGGCCCCGAG TGCCGCCACC 
GTGGTGGACA CCAGCACGCC CAATTGGGCC GCTGCCCTGG GCGACATTAC ACAGGCGCAG 
TGGAAGGAGC GCCGACCAGT GGTGCTGACC CGCTGGCTGT CCGATCTGGA GCCGGCTGGG 
ATACCACAGC CCTTTCAGCC GCTGGAGCGG CATCTCAACA AACACGACGT CTGGAACCTG 
ACGCGGCACA TGCAATCCGA AAGGCAAGCG CAATCGCAGC CGCACGGATT ACTCAAGCGG 
TTGGGGGCTG CCAGTTCGCC CGGCTCCGCT CATCAATCAA ACGCGATTGA GGAGACAGGA 
ACAGGAACCG AAACGGCCAA CGGAGCGCTG GTGCAGCGAC TAATTGAATT GGCACAATGG 
TCCGATTTAA TCGTCTTCGA TTACCTGATC GCGAACCTCG ATCGCGTGGT TAATAACCTG 
TACAACTTTC AATGGAACGC CGACATCATG GCCGCGCCGG CGCACAACCT TGCCCGCCAG 
TCCGCCTCGC AGCTGCTCGT CTTCCTGGAC AACGAAAGCG GCCTGCTGCA CGGCTACCGG 
CTGTTGAAGA AGTACGAGGC ATACCACAGC CTCCTGCTCG ACAACCTGTG CGTCTTCCGG 
CGGCCCACAA TCGACGCCCT GCGACGTTTG CGGGCGGCGG GTGCGGGACG CCGGCTGCGT 
GACCTCTTCG AGCGGACGAC CAGCGCCGGA GTGCGGGATG TTTTACCTTC TCTGCCGGAC 
AAGTCCGTGA AAATCCTGGT GGAGCGCATC GATCGGGTGC TGGGCCAGGT GCAGAAGTGC 
CAGGGCAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477483.1
CDS	1350..3101
Translation	MYDIKRLEAGQQKLQQAQQPLGLDLSGQQQQLTCSVITAPEHRANPNPSSISQSNPSEATHMTLLTLRRRRSLQRRACLLSILAAFVFGMALGVVVPMFGLPRHQDSPPDLPEEQIQMVAVEPLSSYRVEFIKETDELSAEQVFRNAFHLEQDKDAPDSMVVKKLDTNDGSIKEFHVQRTASGRYRKGPERRLSKKMPERVQPQETSRSPTTSPTNPTSEHQAGFIEEDVYWGPTVEQALPKGFAAKDQVSWERFVGEQGRVVRLEQGCGRMQNRMVVFADGTRACARYRQNTDQIQGEIFSYYLGQLLNISNLAPSAATVVDTSTPNWAAALGDITQAQWKERRPVVLTRWLSDLEPAGIPQPFQPLERHLNKHDVWNLTRHMQSERQAQSQPHGLLKRLGAASSPGSAHQSNAIEETGTGTETANGALVQRLIELAQWSDLIVFDYLIANLDRVVNNLYNFQWNADIMAAPAHNLARQSASQLLVFLDNESGLLHGYRLLKKYEAYHSLLLDNLCVFRRPTIDALRRLRAAGAGRRLRDLFERTTSAGVRDVLPSLPDKSVKILVERIDRVLGQVQKCQGS

Target ORF information:

RefSeq Version	NM_058135.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster four-jointed (fj), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058135.4

ORF Insert Sequence:

ATGTACGACA TCAAGCGCCT GGAAGCGGGA CAACAGAAAC TACAGCAAGC ACAGCAACCA 
CTGGGCCTTG ACCTGAGCGG CCAGCAACAG CAACTCACCT GCAGCGTGAT CACAGCGCCC 
GAGCATCGGG CTAATCCCAA TCCCAGCTCC ATTTCGCAAT CCAACCCCAG CGAGGCCACC 
CACATGACTT TGTTGACGCT GCGCCGGCGC CGTTCGCTGC AGCGACGCGC CTGCCTTCTG 
AGCATCCTCG CCGCCTTCGT CTTTGGCATG GCACTGGGCG TTGTTGTGCC CATGTTCGGC 
CTGCCCCGCC ACCAGGACTC CCCGCCAGAT CTGCCGGAGG AGCAGATACA GATGGTTGCC 
GTGGAGCCGC TGAGCAGCTA CCGCGTGGAG TTCATCAAGG AGACGGACGA GCTGAGTGCC 
GAGCAGGTAT TCCGCAATGC CTTCCATCTG GAGCAGGACA AGGATGCTCC GGACTCGATG 
GTGGTGAAGA AATTGGACAC CAACGACGGC AGCATCAAGG AATTCCATGT GCAGCGCACC 
GCCAGCGGTC GTTATCGCAA GGGTCCGGAG AGGAGGCTGT CCAAGAAGAT GCCGGAAAGG 
GTGCAGCCAC AAGAGACCTC TCGTTCTCCG ACGACCTCGC CGACAAATCC TACCAGTGAG 
CACCAAGCGG GATTTATTGA GGAGGACGTC TATTGGGGTC CCACTGTGGA GCAGGCTCTG 
CCCAAGGGAT TCGCCGCCAA GGACCAAGTC TCTTGGGAAC GCTTCGTTGG AGAGCAGGGC 
CGAGTAGTTC GCCTGGAGCA GGGATGCGGA AGAATGCAAA ATCGCATGGT GGTCTTTGCG 
GATGGAACAA GGGCCTGTGC CCGCTACCGC CAGAATACGG ATCAGATTCA GGGCGAGATA 
TTCAGCTACT ACTTGGGCCA GCTCTTAAAC ATAAGCAATC TGGCCCCGAG TGCCGCCACC 
GTGGTGGACA CCAGCACGCC CAATTGGGCC GCTGCCCTGG GCGACATTAC ACAGGCGCAG 
TGGAAGGAGC GCCGACCAGT GGTGCTGACC CGCTGGCTGT CCGATCTGGA GCCGGCTGGG 
ATACCACAGC CCTTTCAGCC GCTGGAGCGG CATCTCAACA AACACGACGT CTGGAACCTG 
ACGCGGCACA TGCAATCCGA AAGGCAAGCG CAATCGCAGC CGCACGGATT ACTCAAGCGG 
TTGGGGGCTG CCAGTTCGCC CGGCTCCGCT CATCAATCAA ACGCGATTGA GGAGACAGGA 
ACAGGAACCG AAACGGCCAA CGGAGCGCTG GTGCAGCGAC TAATTGAATT GGCACAATGG 
TCCGATTTAA TCGTCTTCGA TTACCTGATC GCGAACCTCG ATCGCGTGGT TAATAACCTG 
TACAACTTTC AATGGAACGC CGACATCATG GCCGCGCCGG CGCACAACCT TGCCCGCCAG 
TCCGCCTCGC AGCTGCTCGT CTTCCTGGAC AACGAAAGCG GCCTGCTGCA CGGCTACCGG 
CTGTTGAAGA AGTACGAGGC ATACCACAGC CTCCTGCTCG ACAACCTGTG CGTCTTCCGG 
CGGCCCACAA TCGACGCCCT GCGACGTTTG CGGGCGGCGG GTGCGGGACG CCGGCTGCGT 
GACCTCTTCG AGCGGACGAC CAGCGCCGGA GTGCGGGATG TTTTACCTTC TCTGCCGGAC 
AAGTCCGTGA AAATCCTGGT GGAGCGCATC GATCGGGTGC TGGGCCAGGT GCAGAAGTGC 
CAGGGCAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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fj cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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