PPO1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	PPO1
Entrez Gene ID	37044
Full Name	Prophenoloxidase 1
Synonyms	A1,A[[1]],Bc,CG42639,CG5779,DmePPOA1,Dmel\CG42639,Dmel_CG5779,Dox-A1,DoxA1,Mox,PO,PO A1,PO54,PPO,PPO-1,PROPO-A1,Phox,ProPO,ProPO A1,ProPO54,ProPo,Propo,bc,i12,pro-A,pro-PO,pro-POA[[1]],proPO,proPO A[[1]],proPO-A1,proPO54
General protein information	Preferred Names Prophenoloxidase 1 Names CG42639 gene product from transcript CG42639-RA CG42639-PA PPO1-PA black cell black cells diphenol oxidase A1 subunit monophenol oxidase monophenoloxidase phenoloxidase pro-phenol oxidase A1 prophenol oxidase A1 prophenoloxidase prophenoloxidase 1 prophenoloxidase A1 prophenoloxidase A[[1]]
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 54F6-54F6\|2-80.6 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_057464.4	NP_476812.1	prophenoloxidase 1

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	PPO2	XP_316323.2
Anopheles gambiae (African malaria mosquito)	PPO3	XP_315073.2
Anopheles gambiae (African malaria mosquito)	PPO6	XP_315075.1
Anopheles gambiae (African malaria mosquito)	PPO7	XP_315083.1
Drosophila melanogaster (fruit fly)	proPO-A1	NP_476812.1
Anopheles gambiae (African malaria mosquito)	PPO4	XP_315084.2
Anopheles gambiae (African malaria mosquito)	PPO8	XP_315074.1
Anopheles gambiae (African malaria mosquito)	PPO9	XP_315076.1
Anopheles gambiae (African malaria mosquito)	PPO5	XP_307623.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Plant phenolics are detoxified by prophenoloxidase in the insect gut.
Title: Plant phenolics are detoxified by prophenoloxidase in the insect gut.

PPO1 and PPO2 contribute to the bulk of melanization in the hemolymph, upon septic or clean injury. In contrast, PPO3, is expressed in lamellocytes and contributes to melanization during the encapsulation process.
Title: Drosophila innate immunity: regional and functional specialization of prophenoloxidases.

Genomic insertion of a PPO1(A480V) transgene phenocopies Black cells, proving that A480V is indeed the causal mutation of the historical Black cells phenotype.
Title: The Black cells phenotype is caused by a point mutation in the Drosophila pro-phenoloxidase 1 gene that triggers melanization and hematopoietic defects.

Therefore, rPPO1 can be sequentially cleaved in at least three places by alpha-chymotrypsin to produce activated rPO1(60-kD).
Title: Recombinant Drosophila prophenoloxidase 1 is sequentially cleaved by ?-chymotrypsin during in vitro activation.

An important role for PPO1 and PPO2 in the survival to infection with Gram-positive bacteria and fungi, underlining the importance of melanization in insect host defense.
Title: Prophenoloxidase activation is required for survival to microbial infections in Drosophila.

The following PPO1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPO1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa10413	NM_057464.4 Latest version!	Drosophila melanogaster prophenoloxidase 1 (PPO1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PPO1 ( NM_057464.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476812.1 Drosophila melanogaster prophenoloxidase 1 (PPO1), mRNA.

Price & Availability↑

CloneID	OFa10413
Clone ID Related Accession (Same CDS sequence)	NM_057464.4
Accession Version	NM_057464.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	prophenoloxidase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_057464.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACTAACA CGGATCTGAA AGCCTTGGAA CTTATGTTCC AGCGACCCCT GGAACCGGCA 
TTCACCACTC GTGACTCCGG AAAAACTGTA TTGGAACTGC CCGATTCCTT CTATACGGAT 
CGCTATCGCA ATGACACCGA GGAGGTGGGC AATCGATTCT CTAAGGACGT GGATCTGAAG 
ATCCCAATTC AGGAGTTGAG CAACGTTCCC AGTCTGGAGT TCACCAAAAA GATTGGTCTC 
AAGAATCAAT TTTCGCTGTT TAACAATCGC CATCGTGAGA TCGCCAGCGA GCTGATCACC 
CTCTTCATGA GCGCACCAAA TCTCAGACAA TTCGTGTCGC TTTCCGTTTA CACCAAGGAT 
CGTGTGAATC CTGTGTTGTT CCAATACGCC TATGCCGTCG CCGTTGCCCA TCGCCCGGAT 
ACGCGTGAAG TGCCCATTAC CAACATTTCC CAGATCTTTC CGAGCAACTT TGTAGAACCT 
TCCGCCTTCC GGGATGCCCG TCAGGAAGCT TCTGTTATTG GAGAGAGCGG CGCTCGTGTC 
CATGTGGACA TTCCGCAAAA CTACACGGCC TCGGATCGCG AGGATGAACA GCGCCTGGCG 
TACTTCCGTG AGGACATCGG TGTTAATAGC CATCATTGGC ACTGGCACTT GGTCTACCCG 
ACCACCGGAC CCACGGAGGT GGTCAACAAG GATCGTCGTG GCGAGCTCTT CTACTACATG 
CACCACCAGA TACTCGCGCG CTACAATGTG GAGCGCTTCT GCAACAATCT GAAGAAGGTT 
CAGCCGCTGA ACAATCTGCG TGTGGAGGTT CCCGAGGGCT ACTTCCCCAA GATCCTGTCC 
AGCACGAATA ACCGCACCTA TCCCGCCCGA GTGACCAACC AAAAGCTGAG GGACGTGGAC 
CGCCATGATG GGCGTGTGGA GATCTCCGAT GTGGAGCGCT GGCGTGATCG CGTGCTGGCT 
GCCATAGATC AAGGATACGT TGAAGATTCT TCTGGCAATC GTATACCATT GGACGAAGTT 
CGTGGCATCG ATATCCTGGG CAACATGATT GAGGCATCGC CAGTCCTATC CATCAACTAT 
AATTTCTATG GCAATCTACA CAACGAGGGG CACAACATAA TCTCCTTTGC CCACGATCCC 
GATTACCGTC ACTTGGAGGA ATTCGGTGTG ATGGGCGATG TGACCACGGC CATGAGGGAT 
CCAATCTTCT ACAGGTGGCA CGGATTCATC GACACGGTGT TCAACAAATT CAAGACTCGC 
CTGAACCCGT ACAATGCCGG TGAGCTTAAC TTCGATGGTA TTACCGTGGA CTACATTGAG 
GCCAAGATTG GCAAGTCCAA TACCAAGGCC AACACCCTGT TGACCTACTG GCAGAAGTCG 
AGTGCCGACT TGGCAGCTGG CCTGGACTTT GGACCAACGA CCGATCGCAA CATATTCGCC 
TCGTTCACCC ATCTCCAGAA TGCCCCCTTC ACTTATACAT TCAATGTGAC CAATAATGGA 
GCCAGACGAA CGGGTACTTG CCGTATCTTC ATCTGCCCCA AGGTGGATGA GCGTAATCAG 
GCTTTGAATC TGGAGGAGCA GCGACTGTTG GCCATCGAAA TGGACAAGTT CACAGTTGAT 
TTGGTGCCTG GTGAGAACAC CATACGCCGC CAGTCGACAG AGTCCTCGGT GGCCATTCCC 
TTCGAGCGCT CATTCCGCCC GGTTGGAGCA GACTATCAGC CCAAGGCCGC CGATGAGTTG 
GCACGCTTCA AGTTTTGCGG ATGTGGGTGG CCACAGCATC TCCTGCTGCC CAAGGGCAAT 
GCCCAGGGAA TGGTCTTCGA CCTGTTCGTC ATGATTTCCG ATTACTCGCA GGACTCCGTT 
GAGCAGCCCA AAACACCCAA CGATGCCTGC AGCACGGCGT ACTCGTTCTG TGGTCTGAAG 
GACAAATTGT ATCCTGACAG ACGCACCATG GGCTATCCCT TCGATAGGCG CCTGCCCAAT 
GCCAATCTCA CCGAATTGGT CGGTGCCTTT GGCAACATGG CCAAGACCGA TTTAAGGATT 
GTCTTCAATG ATCGTGTGAT TGACAAGGCC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476812.1
CDS	74..2146
Translation	MTNTDLKALELMFQRPLEPAFTTRDSGKTVLELPDSFYTDRYRNDTEEVGNRFSKDVDLKIPIQELSNVPSLEFTKKIGLKNQFSLFNNRHREIASELITLFMSAPNLRQFVSLSVYTKDRVNPVLFQYAYAVAVAHRPDTREVPITNISQIFPSNFVEPSAFRDARQEASVIGESGARVHVDIPQNYTASDREDEQRLAYFREDIGVNSHHWHWHLVYPTTGPTEVVNKDRRGELFYYMHHQILARYNVERFCNNLKKVQPLNNLRVEVPEGYFPKILSSTNNRTYPARVTNQKLRDVDRHDGRVEISDVERWRDRVLAAIDQGYVEDSSGNRIPLDEVRGIDILGNMIEASPVLSINYNFYGNLHNEGHNIISFAHDPDYRHLEEFGVMGDVTTAMRDPIFYRWHGFIDTVFNKFKTRLNPYNAGELNFDGITVDYIEAKIGKSNTKANTLLTYWQKSSADLAAGLDFGPTTDRNIFASFTHLQNAPFTYTFNVTNNGARRTGTCRIFICPKVDERNQALNLEEQRLLAIEMDKFTVDLVPGENTIRRQSTESSVAIPFERSFRPVGADYQPKAADELARFKFCGCGWPQHLLLPKGNAQGMVFDLFVMISDYSQDSVEQPKTPNDACSTAYSFCGLKDKLYPDRRTMGYPFDRRLPNANLTELVGAFGNMAKTDLRIVFNDRVIDKA

Target ORF information:

RefSeq Version	NM_057464.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster prophenoloxidase 1 (PPO1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057464.4

ORF Insert Sequence:

ATGACTAACA CGGATCTGAA AGCCTTGGAA CTTATGTTCC AGCGACCCCT GGAACCGGCA 
TTCACCACTC GTGACTCCGG AAAAACTGTA TTGGAACTGC CCGATTCCTT CTATACGGAT 
CGCTATCGCA ATGACACCGA GGAGGTGGGC AATCGATTCT CTAAGGACGT GGATCTGAAG 
ATCCCAATTC AGGAGTTGAG CAACGTTCCC AGTCTGGAGT TCACCAAAAA GATTGGTCTC 
AAGAATCAAT TTTCGCTGTT TAACAATCGC CATCGTGAGA TCGCCAGCGA GCTGATCACC 
CTCTTCATGA GCGCACCAAA TCTCAGACAA TTCGTGTCGC TTTCCGTTTA CACCAAGGAT 
CGTGTGAATC CTGTGTTGTT CCAATACGCC TATGCCGTCG CCGTTGCCCA TCGCCCGGAT 
ACGCGTGAAG TGCCCATTAC CAACATTTCC CAGATCTTTC CGAGCAACTT TGTAGAACCT 
TCCGCCTTCC GGGATGCCCG TCAGGAAGCT TCTGTTATTG GAGAGAGCGG CGCTCGTGTC 
CATGTGGACA TTCCGCAAAA CTACACGGCC TCGGATCGCG AGGATGAACA GCGCCTGGCG 
TACTTCCGTG AGGACATCGG TGTTAATAGC CATCATTGGC ACTGGCACTT GGTCTACCCG 
ACCACCGGAC CCACGGAGGT GGTCAACAAG GATCGTCGTG GCGAGCTCTT CTACTACATG 
CACCACCAGA TACTCGCGCG CTACAATGTG GAGCGCTTCT GCAACAATCT GAAGAAGGTT 
CAGCCGCTGA ACAATCTGCG TGTGGAGGTT CCCGAGGGCT ACTTCCCCAA GATCCTGTCC 
AGCACGAATA ACCGCACCTA TCCCGCCCGA GTGACCAACC AAAAGCTGAG GGACGTGGAC 
CGCCATGATG GGCGTGTGGA GATCTCCGAT GTGGAGCGCT GGCGTGATCG CGTGCTGGCT 
GCCATAGATC AAGGATACGT TGAAGATTCT TCTGGCAATC GTATACCATT GGACGAAGTT 
CGTGGCATCG ATATCCTGGG CAACATGATT GAGGCATCGC CAGTCCTATC CATCAACTAT 
AATTTCTATG GCAATCTACA CAACGAGGGG CACAACATAA TCTCCTTTGC CCACGATCCC 
GATTACCGTC ACTTGGAGGA ATTCGGTGTG ATGGGCGATG TGACCACGGC CATGAGGGAT 
CCAATCTTCT ACAGGTGGCA CGGATTCATC GACACGGTGT TCAACAAATT CAAGACTCGC 
CTGAACCCGT ACAATGCCGG TGAGCTTAAC TTCGATGGTA TTACCGTGGA CTACATTGAG 
GCCAAGATTG GCAAGTCCAA TACCAAGGCC AACACCCTGT TGACCTACTG GCAGAAGTCG 
AGTGCCGACT TGGCAGCTGG CCTGGACTTT GGACCAACGA CCGATCGCAA CATATTCGCC 
TCGTTCACCC ATCTCCAGAA TGCCCCCTTC ACTTATACAT TCAATGTGAC CAATAATGGA 
GCCAGACGAA CGGGTACTTG CCGTATCTTC ATCTGCCCCA AGGTGGATGA GCGTAATCAG 
GCTTTGAATC TGGAGGAGCA GCGACTGTTG GCCATCGAAA TGGACAAGTT CACAGTTGAT 
TTGGTGCCTG GTGAGAACAC CATACGCCGC CAGTCGACAG AGTCCTCGGT GGCCATTCCC 
TTCGAGCGCT CATTCCGCCC GGTTGGAGCA GACTATCAGC CCAAGGCCGC CGATGAGTTG 
GCACGCTTCA AGTTTTGCGG ATGTGGGTGG CCACAGCATC TCCTGCTGCC CAAGGGCAAT 
GCCCAGGGAA TGGTCTTCGA CCTGTTCGTC ATGATTTCCG ATTACTCGCA GGACTCCGTT 
GAGCAGCCCA AAACACCCAA CGATGCCTGC AGCACGGCGT ACTCGTTCTG TGGTCTGAAG 
GACAAATTGT ATCCTGACAG ACGCACCATG GGCTATCCCT TCGATAGGCG CCTGCCCAAT 
GCCAATCTCA CCGAATTGGT CGGTGCCTTT GGCAACATGG CCAAGACCGA TTTAAGGATT 
GTCTTCAATG ATCGTGTGAT TGACAAGGCC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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PPO1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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