Dark cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dark
Entrez Gene ID	36914
Full Name	Death-associated APAF1-related killer
Synonyms	APAF-1,APAF1,ARK,Apaf,Apaf-1,Apaf1,Ark,CG6829,D-Apaf-1,DARK,Dapaf-1,Dapaf-1/HAC-1,Dark/Apaf-I,Dark/Dapaf-1/HAC1,Dark/Hac-1/dApaf-1,Dark/Hac-1/dApaf1,Dmel\CG6829,Hac-1,Hac-1/Dark,Hac1,T1,anon-53Fa,apaf-1,apaf1,arc,ark,dAPAF-1,dApaf-1,dApaf-1/DARK/HAC-1,dApaf1,dArk,dapaf,dapaf-1,dapaf-1L,dapaf-1S,dark,dark/dapaf-1/hac-1,dark/hac-1/dapaf-1,hac-1,hac1,l(2)SH0173
General protein information	Preferred Names Death-associated APAF1-related killer Names CG6829 gene product from transcript CG6829-RA Apaf-1 related Apaf-1 related killer Apaf-1-related killer Apaf-1-related-killer CG6829-PA CG6829-PB Dark-PA Dark-PB apaf1-related killer dark drosophila Apaf-1-related killer lethal (2) SH0173 transcription unit 1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 53E4-54A2

mRNA and Protein(s)

mRNA	Protein	Name
NM_166207.3	NP_725637.1	Death-associated APAF1-related killer, isoform B
NM_166208.3	NP_725638.1	Death-associated APAF1-related killer, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study presents a structure of the Dark apoptosome at 4.4 A degrees resolution. A nearly complete atomic model clarifies dATP selectivity during nucleotide exchange, suggests a role for the b-propellers in assembly, and provides a framework to understand apical procaspase activation.
Title: A Near-Atomic Structure of the Dark Apoptosome Provides Insight into Assembly and Activation.

Daxx-like protein of Drosophila interacts with Dmp53 and affects longevity and Ark mRNA level
Title: Daxx-like protein of Drosophila interacts with Dmp53 and affects longevity and Ark mRNA level.

identified helical domain I in the nucleotide oligomerization domain as critical for ARK's apoptotic activity.
Title: ARK, the Apaf-1 related killer in Drosophila, requires diverse domains for its apoptotic activity.

ARK is essential for apoptosis during D. melanogaster development, and for radiation-induced apoptosis. Ark mutant embryos have extra cells, and tissues such as brain lobes and wing discs are enlarged.
Title: The Drosophila melanogaster Apaf-1 homologue ARK is required for most, but not all, programmed cell death.

structural analysis of Apaf-1 in the auto-inhibited form demonstrates the critical role of ADP [review]
Title: Structure of Apaf-1 in the auto-inhibited form: a critical role for ADP.

The following Dark gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dark cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa10303	NM_166208.3 Latest version!	Drosophila melanogaster Death-associated APAF1-related killer (Dark), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa10302	NM_166207.3 Latest version!	Drosophila melanogaster Death-associated APAF1-related killer (Dark), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dark ( NM_166208.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725638.1 Drosophila melanogaster Death-associated APAF1-related killer (Dark), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa10303
Clone ID Related Accession (Same CDS sequence)	NM_166208.3
Accession Version	NM_166208.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1596bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Death-associated APAF1-related killer, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166208.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATTTTG AAACTGGGGA GCACCAATAC CAATACAAGG ACATTTTGTC CGTGTTTGAG 
GATGCATTCG TCGACAACTT CGACTGCAAG GATGTCCAGG ATATGCCGAA ATCAATATTG 
TCCAAGGAGG AAATTGACCA CATCATCATG TCTAAGGATG CAGTCAGCGG CACACTTCGC 
TTGTTTTGGA CACTGCTCTC TAAACAGGAG GAAATGGTCC AGAAATTTGT GGAGGAAGTT 
CTCAGGATCA ACTACAAATT CTTAATGTCG CCAATCAAGA CTGAGCAACG GCAGCCCAGC 
ATGATGACCA GGATGTACAT CGAGCAGAGA GATCGTCTGT ACAACGACAA CCAAGTATTC 
GCCAAGTACA ATGTGAGCCG CCTGCAACCG TATCTGAAGC TAAGGCAGGC TCTTCTAGAA 
CTTCGCCCAG CGAAAAATGT ATTGATTGAT GGCGTCTTGG GATCGGGAAA GACTTGGGTA 
GCCTTGGATG TGTGCCTATC GTACAAAGTG CAATGCAAGA TGGACTTTAA GATCTTCTGG 
CTTAACTTGA AGAACTGCAA TAGCCCGGAG ACCGTGCTAG AGATGCTGCA GAAATTACTT 
TACCAGATCG ATCCCAACTG GACAAGTCGT AGCGATCATA GCAGTAACAT AAAGCTTCGA 
ATCCACAGCA TTCAGGCGGA ACTGCGACGA CTGCTCAAGA GCAAGCCATA CGAGAATTGT 
CTCCTTGTTC TGCTTAATGT GCAAAATGCC AAGGCTTGGA ATGCTTTCAA CTTGAGCTGC 
AAGATATTGC TAACCACACG GTTCAAGCAG GTCACTGACT TTCTTTCGGC AGCTACCACA 
ACTCATATTT CATTGGATCA TCATTCGATG ACGCTTACGC CGGATGAGGT TAAGTCTTTG 
CTTCTAAAGT ACCTCGACTG TCGACCACAG GATTTGCCCC GGGAAGTTTT GACAACGAAT 
CCCCGCAGAC TAAGCATTAT TGCTGAGAGC ATTCGGGATG GTCTGGCGAC CTGGGACAAC 
TGGAAACATG TAAACTGTGA TAAGCTGACG ACCATCATTG AGAGCTCTCT AAACGTTCTG 
GAGCCCGCTG AATATAGAAA AATGTTTGAT AGACTGTCAG TTTTCCCTCC ATCAGCCCAC 
ATACCCACTA TTCTCCTATC GTTGATATGG TTTGATGTTA TCAAATCCGA TGTAATGGTA 
GTGGTTAACA AACTTCATAA ATATTCATTG GTGGAGAAGC AACCAAAAGA GTCAACCATA 
AGTATACCCA GCATATACTT GGAGTTGAAA GTCAAGCTTG AAAACGAATA TGCACTTCAT 
CGGAGTATCG TAGATCACTA TAATATCCCA AAAACATTCG ATTCCGACGA CTTGATACCG 
CCTTACCTGG ACCAATACTT CTATAGCCAC ATCGGTCATC ATCTAAAAAA CATTGAGCAC 
CCCGAACGGA TGACTCTCTT TCGCATGGTC TTTTTAGATT TCCGTTTTCT AGAACAAAAG 
ATTCGGCACG ATTCAACTGC TTGGAATGCC AGTGGATCGA TCTTGAACAC GCTACAGCAG 
CTGAAGTTCT ACAAGCCATA TATTTGCGGT ACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725638.1
CDS	227..1822
Translation	MDFETGEHQYQYKDILSVFEDAFVDNFDCKDVQDMPKSILSKEEIDHIIMSKDAVSGTLRLFWTLLSKQEEMVQKFVEEVLRINYKFLMSPIKTEQRQPSMMTRMYIEQRDRLYNDNQVFAKYNVSRLQPYLKLRQALLELRPAKNVLIDGVLGSGKTWVALDVCLSYKVQCKMDFKIFWLNLKNCNSPETVLEMLQKLLYQIDPNWTSRSDHSSNIKLRIHSIQAELRRLLKSKPYENCLLVLLNVQNAKAWNAFNLSCKILLTTRFKQVTDFLSAATTTHISLDHHSMTLTPDEVKSLLLKYLDCRPQDLPREVLTTNPRRLSIIAESIRDGLATWDNWKHVNCDKLTTIIESSLNVLEPAEYRKMFDRLSVFPPSAHIPTILLSLIWFDVIKSDVMVVVNKLHKYSLVEKQPKESTISIPSIYLELKVKLENEYALHRSIVDHYNIPKTFDSDDLIPPYLDQYFYSHIGHHLKNIEHPERMTLFRMVFLDFRFLEQKIRHDSTAWNASGSILNTLQQLKFYKPYICGT

Target ORF information:

RefSeq Version	NM_166208.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Death-associated APAF1-related killer (Dark), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166208.3

ORF Insert Sequence:

ATGGATTTTG AAACTGGGGA GCACCAATAC CAATACAAGG ACATTTTGTC CGTGTTTGAG 
GATGCATTCG TCGACAACTT CGACTGCAAG GATGTCCAGG ATATGCCGAA ATCAATATTG 
TCCAAGGAGG AAATTGACCA CATCATCATG TCTAAGGATG CAGTCAGCGG CACACTTCGC 
TTGTTTTGGA CACTGCTCTC TAAACAGGAG GAAATGGTCC AGAAATTTGT GGAGGAAGTT 
CTCAGGATCA ACTACAAATT CTTAATGTCG CCAATCAAGA CTGAGCAACG GCAGCCCAGC 
ATGATGACCA GGATGTACAT CGAGCAGAGA GATCGTCTGT ACAACGACAA CCAAGTATTC 
GCCAAGTACA ATGTGAGCCG CCTGCAACCG TATCTGAAGC TAAGGCAGGC TCTTCTAGAA 
CTTCGCCCAG CGAAAAATGT ATTGATTGAT GGCGTCTTGG GATCGGGAAA GACTTGGGTA 
GCCTTGGATG TGTGCCTATC GTACAAAGTG CAATGCAAGA TGGACTTTAA GATCTTCTGG 
CTTAACTTGA AGAACTGCAA TAGCCCGGAG ACCGTGCTAG AGATGCTGCA GAAATTACTT 
TACCAGATCG ATCCCAACTG GACAAGTCGT AGCGATCATA GCAGTAACAT AAAGCTTCGA 
ATCCACAGCA TTCAGGCGGA ACTGCGACGA CTGCTCAAGA GCAAGCCATA CGAGAATTGT 
CTCCTTGTTC TGCTTAATGT GCAAAATGCC AAGGCTTGGA ATGCTTTCAA CTTGAGCTGC 
AAGATATTGC TAACCACACG GTTCAAGCAG GTCACTGACT TTCTTTCGGC AGCTACCACA 
ACTCATATTT CATTGGATCA TCATTCGATG ACGCTTACGC CGGATGAGGT TAAGTCTTTG 
CTTCTAAAGT ACCTCGACTG TCGACCACAG GATTTGCCCC GGGAAGTTTT GACAACGAAT 
CCCCGCAGAC TAAGCATTAT TGCTGAGAGC ATTCGGGATG GTCTGGCGAC CTGGGACAAC 
TGGAAACATG TAAACTGTGA TAAGCTGACG ACCATCATTG AGAGCTCTCT AAACGTTCTG 
GAGCCCGCTG AATATAGAAA AATGTTTGAT AGACTGTCAG TTTTCCCTCC ATCAGCCCAC 
ATACCCACTA TTCTCCTATC GTTGATATGG TTTGATGTTA TCAAATCCGA TGTAATGGTA 
GTGGTTAACA AACTTCATAA ATATTCATTG GTGGAGAAGC AACCAAAAGA GTCAACCATA 
AGTATACCCA GCATATACTT GGAGTTGAAA GTCAAGCTTG AAAACGAATA TGCACTTCAT 
CGGAGTATCG TAGATCACTA TAATATCCCA AAAACATTCG ATTCCGACGA CTTGATACCG 
CCTTACCTGG ACCAATACTT CTATAGCCAC ATCGGTCATC ATCTAAAAAA CATTGAGCAC 
CCCGAACGGA TGACTCTCTT TCGCATGGTC TTTTTAGATT TCCGTTTTCT AGAACAAAAG 
ATTCGGCACG ATTCAACTGC TTGGAATGCC AGTGGATCGA TCTTGAACAC GCTACAGCAG 
CTGAAGTTCT ACAAGCCATA TATTTGCGGT ACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dark ( NM_166207.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725637.1 Drosophila melanogaster Death-associated APAF1-related killer (Dark), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa10302
Clone ID Related Accession (Same CDS sequence)	NM_166207.3
Accession Version	NM_166207.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Death-associated APAF1-related killer, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166207.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATTTTG AAACTGGGGA GCACCAATAC CAATACAAGG ACATTTTGTC CGTGTTTGAG 
GATGCATTCG TCGACAACTT CGACTGCAAG GATGTCCAGG ATATGCCGAA ATCAATATTG 
TCCAAGGAGG AAATTGACCA CATCATCATG TCTAAGGATG CAGTCAGCGG CACACTTCGC 
TTGTTTTGGA CACTGCTCTC TAAACAGGAG GAAATGGTCC AGAAATTTGT GGAGGAAGTT 
CTCAGGATCA ACTACAAATT CTTAATGTCG CCAATCAAGA CTGAGCAACG GCAGCCCAGC 
ATGATGACCA GGATGTACAT CGAGCAGAGA GATCGTCTGT ACAACGACAA CCAAGTATTC 
GCCAAGTACA ATGTGAGCCG CCTGCAACCG TATCTGAAGC TAAGGCAGGC TCTTCTAGAA 
CTTCGCCCAG CGAAAAATGT ATTGATTGAT GGCGTCTTGG GATCGGGAAA GACTTGGGTA 
GCCTTGGATG TGTGCCTATC GTACAAAGTG CAATGCAAGA TGGACTTTAA GATCTTCTGG 
CTTAACTTGA AGAACTGCAA TAGCCCGGAG ACCGTGCTAG AGATGCTGCA GAAATTACTT 
TACCAGATCG ATCCCAACTG GACAAGTCGT AGCGATCATA GCAGTAACAT AAAGCTTCGA 
ATCCACAGCA TTCAGGCGGA ACTGCGACGA CTGCTCAAGA GCAAGCCATA CGAGAATTGT 
CTCCTTGTTC TGCTTAATGT GCAAAATGCC AAGGCTTGGA ATGCTTTCAA CTTGAGCTGC 
AAGATATTGC TAACCACACG GTTCAAGCAG GTCACTGACT TTCTTTCGGC AGCTACCACA 
ACTCATATTT CATTGGATCA TCATTCGATG ACGCTTACGC CGGATGAGGT TAAGTCTTTG 
CTTCTAAAGT ACCTCGACTG TCGACCACAG GATTTGCCCC GGGAAGTTTT GACAACGAAT 
CCCCGCAGAC TAAGCATTAT TGCTGAGAGC ATTCGGGATG GTCTGGCGAC CTGGGACAAC 
TGGAAACATG TAAACTGTGA TAAGCTGACG ACCATCATTG AGAGCTCTCT AAACGTTCTG 
GAGCCCGCTG AATATAGAAA AATGTTTGAT AGACTGTCAG TTTTCCCTCC ATCAGCCCAC 
ATACCCACTA TTCTCCTATC GTTGATATGG TTTGATGTTA TCAAATCCGA TGTAATGGTA 
GTGGTTAACA AACTTCATAA ATATTCATTG GTGGAGAAGC AACCAAAAGA GTCAACCATA 
AGTATACCCA GCATATACTT GGAGTTGAAA GTCAAGCTTG AAAACGAATA TGCACTTCAT 
CGGAGTATCG TAGATCACTA TAATATCCCA AAAACATTCG ATTCCGACGA CTTGATACCG 
CCTTACCTGG ACCAATACTT CTATAGCCAC ATCGGTCATC ATCTAAAAAA CATTGAGCAC 
CCCGAACGGA TGACTCTCTT TCGCATGGTC TTTTTAGATT TCCGTTTTCT AGAACAAAAG 
ATTCGGCACG ATTCAACTGC TTGGAATGCC AGTGGATCGA TCTTGAACAC GCTACAGCAG 
CTGAAGTTCT ACAAGCCATA TATTTGCGAC AATGACCCAA AGTATGAGCG CTTAGTCAAC 
GCTATTCTGG ATTTTTTGCC AAAGATTGAG GAAAATCTTA TTTGTTCAAA GTATACGGAC 
CTGCTTAGGA TTGCCCTGAT GGCCGAGGAT GAGGCGATTT TTGAAGAAGC CCATAAACAA 
GTGCAGCGCT TTGACGATCG GGTTTGGTTT ACTAACCACG GTCGTTTCCA CCAGCACCGT 
CAGATTATAA ACCTGGGTGA TAATGAGGGG CGCCATGCTG TCTACCTTCA CAACGACTTT 
TGCTTGATAG CGCTGGCCAG CGGGCAGATC CTGCTAACCG ATGTTTCGCT GGAGGGAGAG 
GACACCTATT TGCTAAGGGA CGAGAGCGAC AGCAGTGACA TTCTGCGTAT GGCGGTATTT 
AACCAACAGA AGCACTTGAT CACCTTGCAC TGCAACGGCA GCGTAAAACT TTGGTCCCTG 
TGGCCCGATT GTCCAGGAAG GCGCCACAGT GGTGGTAGCA AGCAGCAGCT AGTCAATAGC 
GTGGTTAAAC GCTTTATAGG GAGCTATGCA AATCTGAAAA TTGTTGCTTT CTATTTAAAT 
GAAGACGCCG GATTGCCAGA GGCCAACATC CAACTGCATG TGGCATTCAT CAATGGTGAT 
GTGAGTATTT TGAATTGGGA CGAACAAGAT CAGGAGTTCA AACTGTCGCA CGTGCCTGTT 
TTGAAAACGA TGCAGTCGGG AATACGGTGT TTCGTTCAGG TTTTAAAGCG ATATTACGTG 
GTCTGCACCT CAAATTGCAC ACTTACTGTA TGGGATTTGA CCAACGGTTC CAGCAATACG 
CTGGAACTAC ATGTTTTTAA TGTCGAAAAC GACACGCCGT TGGCCTTAGA TGTATTTGAC 
GAAAGGAGTA AGACCGCCAC CGTGTTGCTG ATTTTTAAGT ACAGCGTATG GCGGCTCAAT 
TTTTTGCCAG GTCTTTCGGT TAGTTTGCAA TCGGAGGCAG TGCAACTGCC AGAAGGAAGT 
TTCATCACCT GCGGAAAGCG TTCGACTGAC GGACGTTACC TACTGCTGGG CACGTCCGAG 
GGACTTATTG TGTATGATCT GAAAATCTCT GATCCCGTGC TACGCAGCAA TGTCAGCGAA 
CATATAGAGT GTGTGGATAT ATACGAGCTG TTTGATCCTG TCTATAAGTA CATTGTTTTG 
TGCGGCGCTA AGGGAAAACA GGTGGTCCAT GTGCACACAT TGCGCTCTGT AAGCGGCTCG 
AATTCACATC AAAACAGGGA AATCGCCTGG GTTCACAGTG CAGATGAGAT AAGCGTGATG 
ACAAAGGCTT GCTTGGAGCC TAATGTCTAT CTGCGTTCTC TGATGGACAT GACCAGAGAG 
CGGACGCAAC TTCTGGCTGT GGACTCCAAG GAGCGCATTC ACCTAATTAA ACCGGCCATT 
TCTAGAATTT CCGAATGGTC CACAATCACA CCCACTCATG CTGCCAGTAA CTGTAAGATA 
AATGCCATAT CCGCCTTTAA CGACGAACAG ATTTTCGTTG GCTATGTGGA CGGAGTGATA 
ATAGATGTTA TTCATGATAC AGCGCTACCT CAGCAGTTTA TAGAGGAGCC CATTGATTAC 
CTCAAACAGG TCAGTCCCAA CATTCTGGTG GCGTCGGCAC ACAGTGCCCA GAAGACTGTG 
ATATTCCAGC TGGAAAAGAT AGATCCGCTG CAACCCAATG ACCAGTGGCC ATTGATGATG 
GATGTTAGTA CCAAGTACGC GTCCTTGCAG GAAGGACAGT ATATCATTTT GTTTTCCGAC 
CACGGAGTCT GTCATCTGGA TATAGCTAAC CCATCTGCAT TCGTGAAACC AAAGGATTCG 
GAGGAATACA TTGTCGGCTT TGATCTAAAG AACAGTCTTC TCTTTCTGGC CTACGAAAAC 
AACATCATCG ATGTATTCCG TTTAATATTC AGCTGTAACC AACTAAGGTA CGAACAGATT 
TGCGAAGAAG AGATCGCCCA GAAGGCCAAG ATCAGCTATT TGGTAGCCAC GGATGATGGT 
ACCATGTTGG CTATGGGTTT CGAAAATGGC ACTTTAGAGC TCTTTGCCGT TGAAAACAGA 
AAAGTTCAAC TGATCTACAG CATTGAAGAG GTCCACGAGC ACTGCATCCG GCAATTGCTA 
TTCTCGCCAT GTAAGTTGCT CCTGATCAGC TGCGCGGAGC AGCTGTGCTT CTGGAATGTG 
ACGCACATGC GCAACAATCA ATTGGAGCGC GAGCAAAAAC GGCGGCGCAG TCGACGCCAT 
AAGCAGCACA GTGTTACACA AGAGGATGCT GTGGATGCGG CGCCCATTGC AGCGGACATC 
GATGTAGATG TGACATTCGT TGCCGACGAG TTTCATCCGG TGAACCGAGG GACTGCGGAA 
CTGTGGCGGA ATAAGCGCGG CAATGCCATC CGTCCGGAAC TCCTGGCCTG CGTTAAATTT 
GTGGGAAATG AAGCTCGTCA GTTCTTCACA GACGCCCACT TCTCCCACTT TTATGCCATA 
GATGACGAGG GTGTCTACTA CCATCTGCAG TTGCTGGAGT TAAGCAGACT TCAACCTCCA 
CCTGACCCAG TTACGTTGGA TATTGCCAAT CAGTATGAAG ACTTAAAAAA TCTTCGAATT 
CTCGACAGCC CACTTATGCA GGATTCGGAT AGCGAGGGTG CTGATGTAGT TGGCAACCTG 
GTTCTGGAGA AGAACGGCGG AGTGGCGAGG GCGACACCAA TTCTGGAGGA GGCCAGTTCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725637.1
CDS	227..4549
Translation	MDFETGEHQYQYKDILSVFEDAFVDNFDCKDVQDMPKSILSKEEIDHIIMSKDAVSGTLRLFWTLLSKQEEMVQKFVEEVLRINYKFLMSPIKTEQRQPSMMTRMYIEQRDRLYNDNQVFAKYNVSRLQPYLKLRQALLELRPAKNVLIDGVLGSGKTWVALDVCLSYKVQCKMDFKIFWLNLKNCNSPETVLEMLQKLLYQIDPNWTSRSDHSSNIKLRIHSIQAELRRLLKSKPYENCLLVLLNVQNAKAWNAFNLSCKILLTTRFKQVTDFLSAATTTHISLDHHSMTLTPDEVKSLLLKYLDCRPQDLPREVLTTNPRRLSIIAESIRDGLATWDNWKHVNCDKLTTIIESSLNVLEPAEYRKMFDRLSVFPPSAHIPTILLSLIWFDVIKSDVMVVVNKLHKYSLVEKQPKESTISIPSIYLELKVKLENEYALHRSIVDHYNIPKTFDSDDLIPPYLDQYFYSHIGHHLKNIEHPERMTLFRMVFLDFRFLEQKIRHDSTAWNASGSILNTLQQLKFYKPYICDNDPKYERLVNAILDFLPKIEENLICSKYTDLLRIALMAEDEAIFEEAHKQVQRFDDRVWFTNHGRFHQHRQIINLGDNEGRHAVYLHNDFCLIALASGQILLTDVSLEGEDTYLLRDESDSSDILRMAVFNQQKHLITLHCNGSVKLWSLWPDCPGRRHSGGSKQQLVNSVVKRFIGSYANLKIVAFYLNEDAGLPEANIQLHVAFINGDVSILNWDEQDQEFKLSHVPVLKTMQSGIRCFVQVLKRYYVVCTSNCTLTVWDLTNGSSNTLELHVFNVENDTPLALDVFDERSKTATVLLIFKYSVWRLNFLPGLSVSLQSEAVQLPEGSFITCGKRSTDGRYLLLGTSEGLIVYDLKISDPVLRSNVSEHIECVDIYELFDPVYKYIVLCGAKGKQVVHVHTLRSVSGSNSHQNREIAWVHSADEISVMTKACLEPNVYLRSLMDMTRERTQLLAVDSKERIHLIKPAISRISEWSTITPTHAASNCKINAISAFNDEQIFVGYVDGVIIDVIHDTALPQQFIEEPIDYLKQVSPNILVASAHSAQKTVIFQLEKIDPLQPNDQWPLMMDVSTKYASLQEGQYIILFSDHGVCHLDIANPSAFVKPKDSEEYIVGFDLKNSLLFLAYENNIIDVFRLIFSCNQLRYEQICEEEIAQKAKISYLVATDDGTMLAMGFENGTLELFAVENRKVQLIYSIEEVHEHCIRQLLFSPCKLLLISCAEQLCFWNVTHMRNNQLEREQKRRRSRRHKQHSVTQEDAVDAAPIAADIDVDVTFVADEFHPVNRGTAELWRNKRGNAIRPELLACVKFVGNEARQFFTDAHFSHFYAIDDEGVYYHLQLLELSRLQPPPDPVTLDIANQYEDLKNLRILDSPLMQDSDSEGADVVGNLVLEKNGGVARATPILEEASS

Target ORF information:

RefSeq Version	NM_166207.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Death-associated APAF1-related killer (Dark), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166207.3

ORF Insert Sequence:

ATGGATTTTG AAACTGGGGA GCACCAATAC CAATACAAGG ACATTTTGTC CGTGTTTGAG 
GATGCATTCG TCGACAACTT CGACTGCAAG GATGTCCAGG ATATGCCGAA ATCAATATTG 
TCCAAGGAGG AAATTGACCA CATCATCATG TCTAAGGATG CAGTCAGCGG CACACTTCGC 
TTGTTTTGGA CACTGCTCTC TAAACAGGAG GAAATGGTCC AGAAATTTGT GGAGGAAGTT 
CTCAGGATCA ACTACAAATT CTTAATGTCG CCAATCAAGA CTGAGCAACG GCAGCCCAGC 
ATGATGACCA GGATGTACAT CGAGCAGAGA GATCGTCTGT ACAACGACAA CCAAGTATTC 
GCCAAGTACA ATGTGAGCCG CCTGCAACCG TATCTGAAGC TAAGGCAGGC TCTTCTAGAA 
CTTCGCCCAG CGAAAAATGT ATTGATTGAT GGCGTCTTGG GATCGGGAAA GACTTGGGTA 
GCCTTGGATG TGTGCCTATC GTACAAAGTG CAATGCAAGA TGGACTTTAA GATCTTCTGG 
CTTAACTTGA AGAACTGCAA TAGCCCGGAG ACCGTGCTAG AGATGCTGCA GAAATTACTT 
TACCAGATCG ATCCCAACTG GACAAGTCGT AGCGATCATA GCAGTAACAT AAAGCTTCGA 
ATCCACAGCA TTCAGGCGGA ACTGCGACGA CTGCTCAAGA GCAAGCCATA CGAGAATTGT 
CTCCTTGTTC TGCTTAATGT GCAAAATGCC AAGGCTTGGA ATGCTTTCAA CTTGAGCTGC 
AAGATATTGC TAACCACACG GTTCAAGCAG GTCACTGACT TTCTTTCGGC AGCTACCACA 
ACTCATATTT CATTGGATCA TCATTCGATG ACGCTTACGC CGGATGAGGT TAAGTCTTTG 
CTTCTAAAGT ACCTCGACTG TCGACCACAG GATTTGCCCC GGGAAGTTTT GACAACGAAT 
CCCCGCAGAC TAAGCATTAT TGCTGAGAGC ATTCGGGATG GTCTGGCGAC CTGGGACAAC 
TGGAAACATG TAAACTGTGA TAAGCTGACG ACCATCATTG AGAGCTCTCT AAACGTTCTG 
GAGCCCGCTG AATATAGAAA AATGTTTGAT AGACTGTCAG TTTTCCCTCC ATCAGCCCAC 
ATACCCACTA TTCTCCTATC GTTGATATGG TTTGATGTTA TCAAATCCGA TGTAATGGTA 
GTGGTTAACA AACTTCATAA ATATTCATTG GTGGAGAAGC AACCAAAAGA GTCAACCATA 
AGTATACCCA GCATATACTT GGAGTTGAAA GTCAAGCTTG AAAACGAATA TGCACTTCAT 
CGGAGTATCG TAGATCACTA TAATATCCCA AAAACATTCG ATTCCGACGA CTTGATACCG 
CCTTACCTGG ACCAATACTT CTATAGCCAC ATCGGTCATC ATCTAAAAAA CATTGAGCAC 
CCCGAACGGA TGACTCTCTT TCGCATGGTC TTTTTAGATT TCCGTTTTCT AGAACAAAAG 
ATTCGGCACG ATTCAACTGC TTGGAATGCC AGTGGATCGA TCTTGAACAC GCTACAGCAG 
CTGAAGTTCT ACAAGCCATA TATTTGCGAC AATGACCCAA AGTATGAGCG CTTAGTCAAC 
GCTATTCTGG ATTTTTTGCC AAAGATTGAG GAAAATCTTA TTTGTTCAAA GTATACGGAC 
CTGCTTAGGA TTGCCCTGAT GGCCGAGGAT GAGGCGATTT TTGAAGAAGC CCATAAACAA 
GTGCAGCGCT TTGACGATCG GGTTTGGTTT ACTAACCACG GTCGTTTCCA CCAGCACCGT 
CAGATTATAA ACCTGGGTGA TAATGAGGGG CGCCATGCTG TCTACCTTCA CAACGACTTT 
TGCTTGATAG CGCTGGCCAG CGGGCAGATC CTGCTAACCG ATGTTTCGCT GGAGGGAGAG 
GACACCTATT TGCTAAGGGA CGAGAGCGAC AGCAGTGACA TTCTGCGTAT GGCGGTATTT 
AACCAACAGA AGCACTTGAT CACCTTGCAC TGCAACGGCA GCGTAAAACT TTGGTCCCTG 
TGGCCCGATT GTCCAGGAAG GCGCCACAGT GGTGGTAGCA AGCAGCAGCT AGTCAATAGC 
GTGGTTAAAC GCTTTATAGG GAGCTATGCA AATCTGAAAA TTGTTGCTTT CTATTTAAAT 
GAAGACGCCG GATTGCCAGA GGCCAACATC CAACTGCATG TGGCATTCAT CAATGGTGAT 
GTGAGTATTT TGAATTGGGA CGAACAAGAT CAGGAGTTCA AACTGTCGCA CGTGCCTGTT 
TTGAAAACGA TGCAGTCGGG AATACGGTGT TTCGTTCAGG TTTTAAAGCG ATATTACGTG 
GTCTGCACCT CAAATTGCAC ACTTACTGTA TGGGATTTGA CCAACGGTTC CAGCAATACG 
CTGGAACTAC ATGTTTTTAA TGTCGAAAAC GACACGCCGT TGGCCTTAGA TGTATTTGAC 
GAAAGGAGTA AGACCGCCAC CGTGTTGCTG ATTTTTAAGT ACAGCGTATG GCGGCTCAAT 
TTTTTGCCAG GTCTTTCGGT TAGTTTGCAA TCGGAGGCAG TGCAACTGCC AGAAGGAAGT 
TTCATCACCT GCGGAAAGCG TTCGACTGAC GGACGTTACC TACTGCTGGG CACGTCCGAG 
GGACTTATTG TGTATGATCT GAAAATCTCT GATCCCGTGC TACGCAGCAA TGTCAGCGAA 
CATATAGAGT GTGTGGATAT ATACGAGCTG TTTGATCCTG TCTATAAGTA CATTGTTTTG 
TGCGGCGCTA AGGGAAAACA GGTGGTCCAT GTGCACACAT TGCGCTCTGT AAGCGGCTCG 
AATTCACATC AAAACAGGGA AATCGCCTGG GTTCACAGTG CAGATGAGAT AAGCGTGATG 
ACAAAGGCTT GCTTGGAGCC TAATGTCTAT CTGCGTTCTC TGATGGACAT GACCAGAGAG 
CGGACGCAAC TTCTGGCTGT GGACTCCAAG GAGCGCATTC ACCTAATTAA ACCGGCCATT 
TCTAGAATTT CCGAATGGTC CACAATCACA CCCACTCATG CTGCCAGTAA CTGTAAGATA 
AATGCCATAT CCGCCTTTAA CGACGAACAG ATTTTCGTTG GCTATGTGGA CGGAGTGATA 
ATAGATGTTA TTCATGATAC AGCGCTACCT CAGCAGTTTA TAGAGGAGCC CATTGATTAC 
CTCAAACAGG TCAGTCCCAA CATTCTGGTG GCGTCGGCAC ACAGTGCCCA GAAGACTGTG 
ATATTCCAGC TGGAAAAGAT AGATCCGCTG CAACCCAATG ACCAGTGGCC ATTGATGATG 
GATGTTAGTA CCAAGTACGC GTCCTTGCAG GAAGGACAGT ATATCATTTT GTTTTCCGAC 
CACGGAGTCT GTCATCTGGA TATAGCTAAC CCATCTGCAT TCGTGAAACC AAAGGATTCG 
GAGGAATACA TTGTCGGCTT TGATCTAAAG AACAGTCTTC TCTTTCTGGC CTACGAAAAC 
AACATCATCG ATGTATTCCG TTTAATATTC AGCTGTAACC AACTAAGGTA CGAACAGATT 
TGCGAAGAAG AGATCGCCCA GAAGGCCAAG ATCAGCTATT TGGTAGCCAC GGATGATGGT 
ACCATGTTGG CTATGGGTTT CGAAAATGGC ACTTTAGAGC TCTTTGCCGT TGAAAACAGA 
AAAGTTCAAC TGATCTACAG CATTGAAGAG GTCCACGAGC ACTGCATCCG GCAATTGCTA 
TTCTCGCCAT GTAAGTTGCT CCTGATCAGC TGCGCGGAGC AGCTGTGCTT CTGGAATGTG 
ACGCACATGC GCAACAATCA ATTGGAGCGC GAGCAAAAAC GGCGGCGCAG TCGACGCCAT 
AAGCAGCACA GTGTTACACA AGAGGATGCT GTGGATGCGG CGCCCATTGC AGCGGACATC 
GATGTAGATG TGACATTCGT TGCCGACGAG TTTCATCCGG TGAACCGAGG GACTGCGGAA 
CTGTGGCGGA ATAAGCGCGG CAATGCCATC CGTCCGGAAC TCCTGGCCTG CGTTAAATTT 
GTGGGAAATG AAGCTCGTCA GTTCTTCACA GACGCCCACT TCTCCCACTT TTATGCCATA 
GATGACGAGG GTGTCTACTA CCATCTGCAG TTGCTGGAGT TAAGCAGACT TCAACCTCCA 
CCTGACCCAG TTACGTTGGA TATTGCCAAT CAGTATGAAG ACTTAAAAAA TCTTCGAATT 
CTCGACAGCC CACTTATGCA GGATTCGGAT AGCGAGGGTG CTGATGTAGT TGGCAACCTG 
GTTCTGGAGA AGAACGGCGG AGTGGCGAGG GCGACACCAA TTCTGGAGGA GGCCAGTTCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Dark cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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