Nach cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nach
Entrez Gene ID	36864
Full Name	CG8178 gene product from transcript CG8178-RA
Synonyms	CG8178,Dmel\CG8178,PPK4,ppk4
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 53C14-53C14

mRNA and Protein(s)

mRNA	Protein	Name
NM_137301.1	NP_611145.1	nach, isoform A
NM_001347816.1	NP_001334723.1	nach, isoform D
NM_001347817.1	NP_001334724.1	nach, isoform E

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nach gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nach cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa10253	NM_137301.1 Latest version!	Drosophila melanogaster nach, transcript variant A (Nach), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa10254	NM_001347816.1 Latest version!	Drosophila melanogaster nach, transcript variant D (Nach), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa10255	NM_001347817.1 Latest version!	Drosophila melanogaster nach, transcript variant E (Nach), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nach ( NM_137301.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611145.1 Drosophila melanogaster nach, transcript variant A (Nach), partial mRNA.

Price & Availability↑

CloneID	OFa10253
Clone ID Related Accession (Same CDS sequence)	NM_137301.1
Accession Version	NM_137301.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nach, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGGCCATC AGGAGGAGCT GAAGCCGGAG CAAGTGGATC TGAAAGTAAC CCCCTTTGTG 
GGCTATCTGC GACGCACATG GAGTGACTTC TGTGCCACCA GCAGTATCCA TGGATTGAAA 
TACACTCGTG ACGAGGATAC GAATAAGATT GTGCACCTGG TGTGGCTCTT AATATCGGTG 
GTGATGTTCA TTTGTGCTGT TGTTATGGCG CGCACCTTCT ACATGGACTA TCGCAGCAGT 
CCGACGCGTA TGAATGTGGA AAGTGATAAC ACACCGGTTA ATAGACTCTA TTTCCCACCT 
GTCACAATTT GTCCAGATGT ACTCTTCAAC ATGCAAAAGT CCGAGGCCTT TCTGAACACT 
CTTCGACTTC CAAAAGGAGC CGAGCTGAGG GGCATTCTAC GCAAACTTCA CATTTTCTAC 
GGTTTTATGC TAGATGATGA GCGCTATTCG GCGGAGGACA TCGAGCAAAT GGAAGCCCTT 
CTATTCCTAA ATAATCTCAC AATTCCGGAA TTTGTAGAAC ACTTACGCTG GAATTGTGAC 
GAAATTCTGT ATCGCTGTAG GTTTAATGGT GAAATAATGG ATTGTTCCAA GATATTTCAG 
CTTTCCAAGA CGTTTTTCGG TCATTGCTGT TCATTCAATT TGAGACAAAA AGGTTTGAAC 
TTCACCGCCC AACGAGCAAT AGGTGGCCTT AGATATGGCC TGTCAGTGGT TGTACGGTAT 
AAAGACGACA ACTACGATCC ATTGCAATCG TATTCGTATG GCGTAAAGTT ATTGATCCAA 
GAAGCAGATG CCTTTCCCAG TGCCCATTCT GCTGCAAAAT TTATAGCCTT CAATTCGGAG 
ACATTTGCTG CAGTTAGACC TCAAGAAACG TTTTGCTCTT CTGCTGTGAA AGCTTTAATC 
ATCGAAGAGA GAAATTGCGT TTTTCAAAAT GAGTTTCCCA TGCGTTACTT TTCCGATTAT 
GTATACCCTA ACTGCGAGCT AAATTGTAGA GTAACGAATA TGGTCAAGTT CTGCGGTTGT 
CACACGTACT TTTTTGATTT TAATCGCACT AGCGATCGGA TTTGTACATT TCGAGATATT 
CCTTGTCTAG TGGATAATTT TGCCAATATA ATTACAAGAA AGAAAAGTAC CCAGTGCTAC 
TGTCCGCTAA CCTGTGAACA CATTGATTAC GACGTACAGT TGACGAATTT TCCCCTGGAA 
CTGAATATGC CGGTGGCCGA TAAGTTCTAT TCGGGCTTAG CCAAAAACGA TGGTGTATTG 
CATGTCTTTA TCAATTCGTT TAGCTACCGC CGATTGCGAC GTGACTTGCT TTCCAATATG 
GTTACTTTGG TGTCTAATCT AGGCAGCGCC TTCAGTCTTT TTGTGGGCAT GAGCATGCTT 
TCGGTGGTAG AAATTATCTA CTATTTTTCA GTAATTTTAC GCAAGAACTA CAAACTGGAA 
TGCGAAACTC GCAGTCAGAT GCTTCACAAG AAGCCGAAGT TCGCGTGGCC CAAAGCTAAT 
GACACTCACA GTAAAGAGCA GAAATCAGTT TTCATAATTC ACAAATCGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611145.1
CDS	1..1551
Translation	MGHQEELKPEQVDLKVTPFVGYLRRTWSDFCATSSIHGLKYTRDEDTNKIVHLVWLLISVVMFICAVVMARTFYMDYRSSPTRMNVESDNTPVNRLYFPPVTICPDVLFNMQKSEAFLNTLRLPKGAELRGILRKLHIFYGFMLDDERYSAEDIEQMEALLFLNNLTIPEFVEHLRWNCDEILYRCRFNGEIMDCSKIFQLSKTFFGHCCSFNLRQKGLNFTAQRAIGGLRYGLSVVVRYKDDNYDPLQSYSYGVKLLIQEADAFPSAHSAAKFIAFNSETFAAVRPQETFCSSAVKALIIEERNCVFQNEFPMRYFSDYVYPNCELNCRVTNMVKFCGCHTYFFDFNRTSDRICTFRDIPCLVDNFANIITRKKSTQCYCPLTCEHIDYDVQLTNFPLELNMPVADKFYSGLAKNDGVLHVFINSFSYRRLRRDLLSNMVTLVSNLGSAFSLFVGMSMLSVVEIIYYFSVILRKNYKLECETRSQMLHKKPKFAWPKANDTHSKEQKSVFIIHKS

Target ORF information:

RefSeq Version	NM_137301.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nach, transcript variant A (Nach), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137301.1

ORF Insert Sequence:

ATGGGCCATC AGGAGGAGCT GAAGCCGGAG CAAGTGGATC TGAAAGTAAC CCCCTTTGTG 
GGCTATCTGC GACGCACATG GAGTGACTTC TGTGCCACCA GCAGTATCCA TGGATTGAAA 
TACACTCGTG ACGAGGATAC GAATAAGATT GTGCACCTGG TGTGGCTCTT AATATCGGTG 
GTGATGTTCA TTTGTGCTGT TGTTATGGCG CGCACCTTCT ACATGGACTA TCGCAGCAGT 
CCGACGCGTA TGAATGTGGA AAGTGATAAC ACACCGGTTA ATAGACTCTA TTTCCCACCT 
GTCACAATTT GTCCAGATGT ACTCTTCAAC ATGCAAAAGT CCGAGGCCTT TCTGAACACT 
CTTCGACTTC CAAAAGGAGC CGAGCTGAGG GGCATTCTAC GCAAACTTCA CATTTTCTAC 
GGTTTTATGC TAGATGATGA GCGCTATTCG GCGGAGGACA TCGAGCAAAT GGAAGCCCTT 
CTATTCCTAA ATAATCTCAC AATTCCGGAA TTTGTAGAAC ACTTACGCTG GAATTGTGAC 
GAAATTCTGT ATCGCTGTAG GTTTAATGGT GAAATAATGG ATTGTTCCAA GATATTTCAG 
CTTTCCAAGA CGTTTTTCGG TCATTGCTGT TCATTCAATT TGAGACAAAA AGGTTTGAAC 
TTCACCGCCC AACGAGCAAT AGGTGGCCTT AGATATGGCC TGTCAGTGGT TGTACGGTAT 
AAAGACGACA ACTACGATCC ATTGCAATCG TATTCGTATG GCGTAAAGTT ATTGATCCAA 
GAAGCAGATG CCTTTCCCAG TGCCCATTCT GCTGCAAAAT TTATAGCCTT CAATTCGGAG 
ACATTTGCTG CAGTTAGACC TCAAGAAACG TTTTGCTCTT CTGCTGTGAA AGCTTTAATC 
ATCGAAGAGA GAAATTGCGT TTTTCAAAAT GAGTTTCCCA TGCGTTACTT TTCCGATTAT 
GTATACCCTA ACTGCGAGCT AAATTGTAGA GTAACGAATA TGGTCAAGTT CTGCGGTTGT 
CACACGTACT TTTTTGATTT TAATCGCACT AGCGATCGGA TTTGTACATT TCGAGATATT 
CCTTGTCTAG TGGATAATTT TGCCAATATA ATTACAAGAA AGAAAAGTAC CCAGTGCTAC 
TGTCCGCTAA CCTGTGAACA CATTGATTAC GACGTACAGT TGACGAATTT TCCCCTGGAA 
CTGAATATGC CGGTGGCCGA TAAGTTCTAT TCGGGCTTAG CCAAAAACGA TGGTGTATTG 
CATGTCTTTA TCAATTCGTT TAGCTACCGC CGATTGCGAC GTGACTTGCT TTCCAATATG 
GTTACTTTGG TGTCTAATCT AGGCAGCGCC TTCAGTCTTT TTGTGGGCAT GAGCATGCTT 
TCGGTGGTAG AAATTATCTA CTATTTTTCA GTAATTTTAC GCAAGAACTA CAAACTGGAA 
TGCGAAACTC GCAGTCAGAT GCTTCACAAG AAGCCGAAGT TCGCGTGGCC CAAAGCTAAT 
GACACTCACA GTAAAGAGCA GAAATCAGTT TTCATAATTC ACAAATCGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nach ( NM_001347816.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001334723.1 Drosophila melanogaster nach, transcript variant D (Nach), partial mRNA.

Price & Availability↑

CloneID	OFa10254
Clone ID Related Accession (Same CDS sequence)	NM_001347816.1
Accession Version	NM_001347816.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1608bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nach, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGGCCATC AGGAGGAGCT GAAGCCGGAG CAAGTGGATC TGAAAGTAAC CCCCTTTGTG 
GGCTATCTGC GACGCACATG GAGTGACTTC TGTGCCACCA GCAGTATCCA TGGATTGAAA 
TACACTCGTG ACGAGGATAC GAATAAGATT GTGCACCTGG TGTGGCTCTT AATATCGGTG 
GTGATGTTCA TTTGTGCTGT TGTTATGGCG CGCACCTTCT ACATGGACTA TCGCAGCAGT 
CCGACGCGTA TGAATGTGGA AAGTGATAAC ACACCGGTTA ATAGACTCTA TTTCCCACCT 
GTCACAATTT GTCCAGATGT ACTCTTCAAC ATGCAAAAGT CCGAGGCCTT TCTGAACACT 
CTTCGACTTC CAAAAGGAGC CGAGCTGAGG GGCATTCTAC GCAAACTTCA CATTTTCTAC 
GGTTTTATGC TAGATGATGA GCGCTATTCG GCGGAGGACA TCGAGCAAAT GGAAGCCCTT 
CTATTCCTAA ATAATCTCAC AATTCCGGAA TTTGTAGAAC ACTTACGCTG GAATTGTGAC 
GAAATTCTGT ATCGCTGTAG GTTTAATGGT GAAATAATGG ATTGTTCCAA GATATTTCAG 
CTTTCCAAGA CGTTTTTCGG TCATTGCTGT TCATTCAATT TGAGACAAAA AGGGTGGGTA 
AATAACAAAC TAAATAATTT AGAGAGTTTT AAAGTTTTCC ATTTGAACAG TTTGAACTTC 
ACCGCCCAAC GAGCAATAGG TGGCCTTAGA TATGGCCTGT CAGTGGTTGT ACGGTATAAA 
GACGACAACT ACGATCCATT GCAATCGTAT TCGTATGGCG TAAAGTTATT GATCCAAGAA 
GCAGATGCCT TTCCCAGTGC CCATTCTGCT GCAAAATTTA TAGCCTTCAA TTCGGAGACA 
TTTGCTGCAG TTAGACCTCA AGAAACGTTT TGCTCTTCTG CTGTGAAAGC TTTAATCATC 
GAAGAGAGAA ATTGCGTTTT TCAAAATGAG TTTCCCATGC GTTACTTTTC CGATTATGTA 
TACCCTAACT GCGAGCTAAA TTGTAGAGTA ACGAATATGG TCAAGTTCTG CGGTTGTCAC 
ACGTACTTTT TTGATTTTAA TCGCACTAGC GATCGGATTT GTACATTTCG AGATATTCCT 
TGTCTAGTGG ATAATTTTGC CAATATAATT ACAAGAAAGA AAAGTACCCA GTGCTACTGT 
CCGCTAACCT GTGAACACAT TGATTACGAC GTACAGTTGA CGAATTTTCC CCTGGAACTG 
AATATGCCGG TGGCCGATAA GTTCTATTCG GGCTTAGCCA AAAACGATGG TGTATTGCAT 
GTCTTTATCA ATTCGTTTAG CTACCGCCGA TTGCGACGTG ACTTGCTTTC CAATATGGTT 
ACTTTGGTGT CTAATCTAGG CAGCGCCTTC AGTCTTTTTG TGGGCATGAG CATGCTTTCG 
GTGGTAGAAA TTATCTACTA TTTTTCAGTA ATTTTACGCA AGAACTACAA ACTGGAATGC 
GAAACTCGCA GTCAGATGCT TCACAAGAAG CCGAAGTTCG CGTGGCCCAA AGCTAATGAC 
ACTCACAGTA AAGAGCAGAA ATCAGTTTTC ATAATTCACA AATCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001334723.1
CDS	1..1608
Translation	MGHQEELKPEQVDLKVTPFVGYLRRTWSDFCATSSIHGLKYTRDEDTNKIVHLVWLLISVVMFICAVVMARTFYMDYRSSPTRMNVESDNTPVNRLYFPPVTICPDVLFNMQKSEAFLNTLRLPKGAELRGILRKLHIFYGFMLDDERYSAEDIEQMEALLFLNNLTIPEFVEHLRWNCDEILYRCRFNGEIMDCSKIFQLSKTFFGHCCSFNLRQKGWVNNKLNNLESFKVFHLNSLNFTAQRAIGGLRYGLSVVVRYKDDNYDPLQSYSYGVKLLIQEADAFPSAHSAAKFIAFNSETFAAVRPQETFCSSAVKALIIEERNCVFQNEFPMRYFSDYVYPNCELNCRVTNMVKFCGCHTYFFDFNRTSDRICTFRDIPCLVDNFANIITRKKSTQCYCPLTCEHIDYDVQLTNFPLELNMPVADKFYSGLAKNDGVLHVFINSFSYRRLRRDLLSNMVTLVSNLGSAFSLFVGMSMLSVVEIIYYFSVILRKNYKLECETRSQMLHKKPKFAWPKANDTHSKEQKSVFIIHKS

Target ORF information:

RefSeq Version	NM_001347816.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nach, transcript variant D (Nach), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001347816.1

ORF Insert Sequence:

ATGGGCCATC AGGAGGAGCT GAAGCCGGAG CAAGTGGATC TGAAAGTAAC CCCCTTTGTG 
GGCTATCTGC GACGCACATG GAGTGACTTC TGTGCCACCA GCAGTATCCA TGGATTGAAA 
TACACTCGTG ACGAGGATAC GAATAAGATT GTGCACCTGG TGTGGCTCTT AATATCGGTG 
GTGATGTTCA TTTGTGCTGT TGTTATGGCG CGCACCTTCT ACATGGACTA TCGCAGCAGT 
CCGACGCGTA TGAATGTGGA AAGTGATAAC ACACCGGTTA ATAGACTCTA TTTCCCACCT 
GTCACAATTT GTCCAGATGT ACTCTTCAAC ATGCAAAAGT CCGAGGCCTT TCTGAACACT 
CTTCGACTTC CAAAAGGAGC CGAGCTGAGG GGCATTCTAC GCAAACTTCA CATTTTCTAC 
GGTTTTATGC TAGATGATGA GCGCTATTCG GCGGAGGACA TCGAGCAAAT GGAAGCCCTT 
CTATTCCTAA ATAATCTCAC AATTCCGGAA TTTGTAGAAC ACTTACGCTG GAATTGTGAC 
GAAATTCTGT ATCGCTGTAG GTTTAATGGT GAAATAATGG ATTGTTCCAA GATATTTCAG 
CTTTCCAAGA CGTTTTTCGG TCATTGCTGT TCATTCAATT TGAGACAAAA AGGGTGGGTA 
AATAACAAAC TAAATAATTT AGAGAGTTTT AAAGTTTTCC ATTTGAACAG TTTGAACTTC 
ACCGCCCAAC GAGCAATAGG TGGCCTTAGA TATGGCCTGT CAGTGGTTGT ACGGTATAAA 
GACGACAACT ACGATCCATT GCAATCGTAT TCGTATGGCG TAAAGTTATT GATCCAAGAA 
GCAGATGCCT TTCCCAGTGC CCATTCTGCT GCAAAATTTA TAGCCTTCAA TTCGGAGACA 
TTTGCTGCAG TTAGACCTCA AGAAACGTTT TGCTCTTCTG CTGTGAAAGC TTTAATCATC 
GAAGAGAGAA ATTGCGTTTT TCAAAATGAG TTTCCCATGC GTTACTTTTC CGATTATGTA 
TACCCTAACT GCGAGCTAAA TTGTAGAGTA ACGAATATGG TCAAGTTCTG CGGTTGTCAC 
ACGTACTTTT TTGATTTTAA TCGCACTAGC GATCGGATTT GTACATTTCG AGATATTCCT 
TGTCTAGTGG ATAATTTTGC CAATATAATT ACAAGAAAGA AAAGTACCCA GTGCTACTGT 
CCGCTAACCT GTGAACACAT TGATTACGAC GTACAGTTGA CGAATTTTCC CCTGGAACTG 
AATATGCCGG TGGCCGATAA GTTCTATTCG GGCTTAGCCA AAAACGATGG TGTATTGCAT 
GTCTTTATCA ATTCGTTTAG CTACCGCCGA TTGCGACGTG ACTTGCTTTC CAATATGGTT 
ACTTTGGTGT CTAATCTAGG CAGCGCCTTC AGTCTTTTTG TGGGCATGAG CATGCTTTCG 
GTGGTAGAAA TTATCTACTA TTTTTCAGTA ATTTTACGCA AGAACTACAA ACTGGAATGC 
GAAACTCGCA GTCAGATGCT TCACAAGAAG CCGAAGTTCG CGTGGCCCAA AGCTAATGAC 
ACTCACAGTA AAGAGCAGAA ATCAGTTTTC ATAATTCACA AATCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nach ( NM_001347817.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001334724.1 Drosophila melanogaster nach, transcript variant E (Nach), partial mRNA.

Price & Availability↑

CloneID	OFa10255
Clone ID Related Accession (Same CDS sequence)	NM_001347817.1
Accession Version	NM_001347817.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nach, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGGCCATC AGGAGGAGCT GAAGCCGGAG CAAGTGGATC TGAAAGTAAC CCCCTTTGTG 
GGCTATCTGC GACGCACATG GAGTGACTTC TGTGCCACCA GCAGTATCCA TGGATTGAAA 
TACACTCGTG ACGAGGATAC GAATAAGATT GTGCACCTGG TGTGGCTCTT AATATCGGTG 
GTGATGTTCA TTTGTGCTGT TGTTATGGCG CGCACCTTCT ACATGGACTA TCGCAGCAGT 
CCGACGCGTA TGAATGTGGA AAGTGATAAC ACACCGGTTA ATAGACTCTA TTTCCCACCT 
GTCACAATTT GTCCAGATGT ACTCTTCAAC ATGCAAAAGT CCGAGGCCTT TCTGAACACT 
CTTCGACTTC CAAAAGGAGC CGAGCTGAGG GGCATTCTAC GCAAACTTCA CATTTTCTAC 
GGTTTTATGC TAGATGATGA GCGCTATTCG GCGGAGGACA TCGAGCAAAT GGAAGCCCTT 
CTATTCCTAA ATAATCTCAC AATTCCGGAA TTTGTAGAAC ACTTACGCTG GAATTGTGAC 
GAAATTCTGT ATCGCTGTAG GTTTAATGGT GAAATAATGG ATTGTTCCAA GATATTTCAG 
CTTTCCAAGA CGTTTTTCGG TCATTGCTGT TCATTCAATT TGAGACAAAA AGGGTGGGTA 
AATAACAAAC TAAATAATTT AGAGAGTTTT AAAGTTTTCC ATTTGAACAG TTTGAACTTC 
ACCGCCCAAC GAGCAATAGG TGGCCTTAGA TATGGCCTGT CAGTGGTTGT ACGGTATAAA 
GACGACAACT ACGATCCATT GCAATCGTAT TCGTATGGCG TAAAGTTATT GATCCAAGAA 
GCAGATGCCT TTCCCAGTGC CCATTCTGCT GCAAAATTTA TAGCCTTCAA TTCGGAGACA 
TTTGCTGCAG TTAGACCTCA AGAAACGTTT TGCTCTTCTG CTGTGAAAGC TTTAATCATC 
GAAGAGAGAA ATTGCGTTTT TCAAAATGAG TTTCCCATGC GTTACTTTTC CGATTATGTA 
TACCCTAACT GCGAGCTAAA TTGTAGAGTA ACGAATATGG TCAAGTTCTG CGGTTGTCAC 
ACGTACTTTT TTGATTTTAA TCGCACTAGC GATCGGATTT GTACATTTCG AGATATTCCT 
TGTCTAGTGG ATAATTTTGG TAGGTTATTA TTGATTTGCT CAAAATATAG CGATTTTAAT 
AGATTGCTTT CAGCCAATAT AATTACAAGA AAGAAAAGTA CCCAGTGCTA CTGTCCGCTA 
ACCTGTGAAC ACATTGATTA CGACGTACAG TTGACGAATT TTCCCCTGGA ACTGAATATG 
CCGGTGGCCG ATAAGTTCTA TTCGGGCTTA GCCAAAAACG ATGGTGTATT GCATGTCTTT 
ATCAATTCGT TTAGCTACCG CCGATTGCGA CGTGACTTGC TTTCCAATAT GGTTACTTTG 
GTGTCTAATC TAGGCAGCGC CTTCAGTCTT TTTGTGGGCA TGAGCATGCT TTCGGTGGTA 
GAAATTATCT ACTATTTTTC AGTAATTTTA CGCAAGAACT ACAAACTGGA ATGCGAAACT 
CGCAGTCAGA TGCTTCACAA GAAGCCGAAG TTCGCGTGGC CCAAAGCTAA TGACACTCAC 
AGTAAAGAGC AGAAATCAGT TTTCATAATT CACAAATCGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001334724.1
CDS	1..1662
Translation	MGHQEELKPEQVDLKVTPFVGYLRRTWSDFCATSSIHGLKYTRDEDTNKIVHLVWLLISVVMFICAVVMARTFYMDYRSSPTRMNVESDNTPVNRLYFPPVTICPDVLFNMQKSEAFLNTLRLPKGAELRGILRKLHIFYGFMLDDERYSAEDIEQMEALLFLNNLTIPEFVEHLRWNCDEILYRCRFNGEIMDCSKIFQLSKTFFGHCCSFNLRQKGWVNNKLNNLESFKVFHLNSLNFTAQRAIGGLRYGLSVVVRYKDDNYDPLQSYSYGVKLLIQEADAFPSAHSAAKFIAFNSETFAAVRPQETFCSSAVKALIIEERNCVFQNEFPMRYFSDYVYPNCELNCRVTNMVKFCGCHTYFFDFNRTSDRICTFRDIPCLVDNFGRLLLICSKYSDFNRLLSANIITRKKSTQCYCPLTCEHIDYDVQLTNFPLELNMPVADKFYSGLAKNDGVLHVFINSFSYRRLRRDLLSNMVTLVSNLGSAFSLFVGMSMLSVVEIIYYFSVILRKNYKLECETRSQMLHKKPKFAWPKANDTHSKEQKSVFIIHKS

Target ORF information:

RefSeq Version	NM_001347817.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nach, transcript variant E (Nach), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001347817.1

ORF Insert Sequence:

ATGGGCCATC AGGAGGAGCT GAAGCCGGAG CAAGTGGATC TGAAAGTAAC CCCCTTTGTG 
GGCTATCTGC GACGCACATG GAGTGACTTC TGTGCCACCA GCAGTATCCA TGGATTGAAA 
TACACTCGTG ACGAGGATAC GAATAAGATT GTGCACCTGG TGTGGCTCTT AATATCGGTG 
GTGATGTTCA TTTGTGCTGT TGTTATGGCG CGCACCTTCT ACATGGACTA TCGCAGCAGT 
CCGACGCGTA TGAATGTGGA AAGTGATAAC ACACCGGTTA ATAGACTCTA TTTCCCACCT 
GTCACAATTT GTCCAGATGT ACTCTTCAAC ATGCAAAAGT CCGAGGCCTT TCTGAACACT 
CTTCGACTTC CAAAAGGAGC CGAGCTGAGG GGCATTCTAC GCAAACTTCA CATTTTCTAC 
GGTTTTATGC TAGATGATGA GCGCTATTCG GCGGAGGACA TCGAGCAAAT GGAAGCCCTT 
CTATTCCTAA ATAATCTCAC AATTCCGGAA TTTGTAGAAC ACTTACGCTG GAATTGTGAC 
GAAATTCTGT ATCGCTGTAG GTTTAATGGT GAAATAATGG ATTGTTCCAA GATATTTCAG 
CTTTCCAAGA CGTTTTTCGG TCATTGCTGT TCATTCAATT TGAGACAAAA AGGGTGGGTA 
AATAACAAAC TAAATAATTT AGAGAGTTTT AAAGTTTTCC ATTTGAACAG TTTGAACTTC 
ACCGCCCAAC GAGCAATAGG TGGCCTTAGA TATGGCCTGT CAGTGGTTGT ACGGTATAAA 
GACGACAACT ACGATCCATT GCAATCGTAT TCGTATGGCG TAAAGTTATT GATCCAAGAA 
GCAGATGCCT TTCCCAGTGC CCATTCTGCT GCAAAATTTA TAGCCTTCAA TTCGGAGACA 
TTTGCTGCAG TTAGACCTCA AGAAACGTTT TGCTCTTCTG CTGTGAAAGC TTTAATCATC 
GAAGAGAGAA ATTGCGTTTT TCAAAATGAG TTTCCCATGC GTTACTTTTC CGATTATGTA 
TACCCTAACT GCGAGCTAAA TTGTAGAGTA ACGAATATGG TCAAGTTCTG CGGTTGTCAC 
ACGTACTTTT TTGATTTTAA TCGCACTAGC GATCGGATTT GTACATTTCG AGATATTCCT 
TGTCTAGTGG ATAATTTTGG TAGGTTATTA TTGATTTGCT CAAAATATAG CGATTTTAAT 
AGATTGCTTT CAGCCAATAT AATTACAAGA AAGAAAAGTA CCCAGTGCTA CTGTCCGCTA 
ACCTGTGAAC ACATTGATTA CGACGTACAG TTGACGAATT TTCCCCTGGA ACTGAATATG 
CCGGTGGCCG ATAAGTTCTA TTCGGGCTTA GCCAAAAACG ATGGTGTATT GCATGTCTTT 
ATCAATTCGT TTAGCTACCG CCGATTGCGA CGTGACTTGC TTTCCAATAT GGTTACTTTG 
GTGTCTAATC TAGGCAGCGC CTTCAGTCTT TTTGTGGGCA TGAGCATGCT TTCGGTGGTA 
GAAATTATCT ACTATTTTTC AGTAATTTTA CGCAAGAACT ACAAACTGGA ATGCGAAACT 
CGCAGTCAGA TGCTTCACAA GAAGCCGAAG TTCGCGTGGC CCAAAGCTAA TGACACTCAC 
AGTAAAGAGC AGAAATCAGT TTTCATAATT CACAAATCGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nach cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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