Sema2a cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sema2a
Entrez Gene ID	36846
Full Name	Semaphorin 2a
Synonyms	112/40,BcDNA:LD09561,CG4700,CT14922,D-Sema II,D-Sema-II,D-Sema2a,D-SemaII,D-semaII,Dmel\CG4700,Dsema-II,Sema-2a,SemaII,dSema-II,l(2)03021,sema 2a,sema II,sema-2a,sema-II,sema2a,semaII
General protein information	Preferred Names Semaphorin 2a Names CG4700 gene product from transcript CG4700-RC CG4700-PA CG4700-PB CG4700-PC CG4700-PE D-Semaphorin II Sema-2a Sema2a-PA Sema2a-PB Sema2a-PC Sema2a-PE semaphorin semaphorin 2a semaphorin II semiphorin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 53C6-53C7

mRNA and Protein(s)

mRNA	Protein	Name
NM_166178.3	NP_725586.1	semaphorin 2a, isoform C
NM_134303.4	NP_599130.1	semaphorin 2a, isoform B
NM_058159.5	NP_477507.1	semaphorin 2a, isoform A
NM_206134.2	NP_995856.2	semaphorin 2a, isoform E

Pathways from BioSystems

Homologies

	LOC708263	XP_001096722.1
Bos taurus (cattle)	SEMA3A	NP_001263630.1
Pan troglodytes (chimpanzee)	SEMA3A	XP_001160854.1
Danio rerio (zebrafish)	sema3ab	NP_571136.1
Drosophila melanogaster (fruit fly)	Sema-2a	NP_477507.1
Canis lupus familiaris (dog)	SEMA3A	XP_849675.1
Homo sapiens (human)	SEMA3A	NP_006071.1
Mus musculus (house mouse)	Sema3a	NP_001230002.1
Rattus norvegicus (Norway rat)	Sema3a	NP_059006.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006634	XP_316666.4
Gallus gallus (chicken)	SEMA3A	NP_990308.2
Xenopus tropicalis (tropical clawed frog)	sema3a	XP_004913087.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data support a model in which Sema2a, while being secreted from oenocytes, represses axon extension from the sensory neuron cell body, but only in regions in direct contact with oenocytes.
Title: Semaphorin 2a secreted by oenocytes signals through plexin B and plexin A to guide sensory axons in the Drosophila embryo.

PlexB is a receptor for the secreted semaphorin Sema-2a
Title: Drosophila Plexin B is a Sema-2a receptor required for axon guidance.

The following Sema2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sema2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa10230	NM_166178.3 Latest version!	Drosophila melanogaster semaphorin 2a, transcript variant C (Sema2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa10231	NM_134303.4 Latest version!	Drosophila melanogaster semaphorin 2a, transcript variant B (Sema2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa10231	NM_206134.2 Latest version!	Drosophila melanogaster semaphorin 2a, transcript variant E (Sema2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa10231	NM_058159.5 Latest version!	Drosophila melanogaster semaphorin 2a, transcript variant A (Sema2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sema2a ( NM_166178.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725586.1 Drosophila melanogaster semaphorin 2a, transcript variant C (Sema2a), mRNA.

Price & Availability↑

CloneID	OFa10230
Clone ID Related Accession (Same CDS sequence)	NM_166178.3
Accession Version	NM_166178.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	semaphorin 2a, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166178.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACGGGA TACATTGGCT TCTGTTTGGC AGTTGCCTCA TGCTAATTTC GCAAATAGAG 
GCAGTCACCG AAGAACTTTC ACCAGATCAT GTGCGGGAGT TCAACTGCGG CAAGCTGTAC 
TATCGTACAT TCCATATGAA CGAAGATCGA GATACGCTCT ATGTGGGAGC CATGGATCGC 
GTATTCCGTG TGAACCTGCA GAATATCTCC TCATCCAATT GTAATCGGGA TGTGATCAAC 
TTGGAGCCAA CACGGGATGA TGTGGTTAGC TGCGTCTCCA AAGGCAAAAG TCAGATCTTC 
GACTGCAAGA ACCATGTGCG TGTCATCCAG TCAATGGACC AGGGGGATAG GCTCTATGTA 
TGCGGCACCA ACGCCCACAA TCCCAAGGAT TATGTTATCT ATGCGAATCT AACCCACCTG 
CCGCGCTCGG AATATGTGAT TGGCGTGGGT CTGGGCATTG CCAAGTGCCC CTACGATCCC 
CTCGACAACT CAACTGCGAT TTATGTGGAG AATGGCAATC CGGGTGGTCT GCCCGGTTTG 
TACTCCGGCA CCAATGCGGA GTTCACCAAG GCGGATACGG TTATTTTCCG CACTGATCTG 
TATAATACTT CGGCTAAACG TTTGGAATAT AAATTCAAGA GGACTCTGAA ATACGACTCC 
AAGTGGTTGG ACAAACCAAA CTTTGTCGGC TCCTTTGATA TTGGGGAGTA CGTGTATTTC 
TTTTTCCGTG AAACCGCCGT GGAATACATC AACTGCGGCA AGGCTGTCTA TTCGCGCATC 
GCACGGGTGT GCAAGAAGGA TGTGGGTGGA AAGAATCTGC TGGCCCACAA CTGGGCCACC 
TACCTGAAGG CCAGACTCAA CTGCAGCATC TCCGGCGAAT TTCCGTTCTA TTTCAACGAG 
ATCCAATCGG TCTACCAGCT GCCCTCCGAT AAGAGTCGAT TCTTCGCCAC ATTCACGACG 
AGCACTAATG GCCTGATTGG ATCTGCCGTA TGCAGTTTCC ACATTAACGA GATTCAGGCT 
GCCTTCAATG GCAAATTCAA GGAGCAATCT TCATCGAATT CCGCATGGCT GCCGGTGCTT 
AACTCCCGGG TGCCGGAACC ACGGCCGGGT ACATGTGTCA ACGATACATC AAACCTGCCC 
GATACCGTAC TGAATTTCAT CAGATCCCAT CCACTTATGG ACAAAGCCGT AAATCACGAG 
CACAACAATC CAGTCTATTA TAAAAGGGAT TTGGTCTTCA CCAAGCTCGT CGTTGACAAA 
ATTCGCATTG ACATCCTCAA TCAGGAATAC ATTGTGTACT ATGTGGGCAC CAATCTGGGT 
CGCATTTACA AAATCGTGCA GTACTACCGT AACGGAGAGT CGCTGTCCAA GCTGCTGGAT 
ATCTTCGAGG TGGCTCCAAA CGAGGCCATC CAAGTGATGG AAATCAGCCA GACACGTAAG 
AGCCTCTACA TTGGCACCGA TCATCGCATC AAGCAAATCG ACCTGGCCAT GTGCAATCGC 
CGTTACGACA ACTGCTTCCG CTGCGTCCGT GATCCCTACT GCGGCTGGGA TAAGGAGGCC 
AATACGTGCC GACCGTACGA GCTGGATTTA CTGCAGGATG TGGCCAATGA AACGAGTGAC 
ATTTGCGATT CGAGTGTGCT GAAAAAGAAG ATTGTGGTGA CCTATGGCCA GAGTGTACAT 
CTGGGCTGTT TCGTCAAAAT ACCCGAAGTG CTGAAGAATG AGCAAGTGAC CTGGTATCAT 
CACTCCAAGG ACAAGGGACG CTACGAGATT CGTTACTCGC CGACCAAATA CATTGAGACC 
ACCGAACGTG GCCTGGTTGT GGTTTCCGTG AACGAAGCCG ATGGTGGTCG GTACGATTGC 
CATTTGGGCG GCTCGCTTTT GTGCAGCTAC AACATTACGG TGGATGCCCA CAGATGCACT 
CCGCCGAACA AGAGTAATGA CTATCAGAAA ATCTACTCGG ACTGGTGCCA CGAGTTCGAG 
AAATACAAAA CAGCAATGAA GTCCTGGGAA AAGAAGCAAG CGCAATGCTC GACACGGCAG 
AACTTCAGCA GCAATCAGCA TCCGAATGAG ATTTTCCGTA AGCCCAATGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725586.1
CDS	396..2489
Translation	MNGIHWLLFGSCLMLISQIEAVTEELSPDHVREFNCGKLYYRTFHMNEDRDTLYVGAMDRVFRVNLQNISSSNCNRDVINLEPTRDDVVSCVSKGKSQIFDCKNHVRVIQSMDQGDRLYVCGTNAHNPKDYVIYANLTHLPRSEYVIGVGLGIAKCPYDPLDNSTAIYVENGNPGGLPGLYSGTNAEFTKADTVIFRTDLYNTSAKRLEYKFKRTLKYDSKWLDKPNFVGSFDIGEYVYFFFRETAVEYINCGKAVYSRIARVCKKDVGGKNLLAHNWATYLKARLNCSISGEFPFYFNEIQSVYQLPSDKSRFFATFTTSTNGLIGSAVCSFHINEIQAAFNGKFKEQSSSNSAWLPVLNSRVPEPRPGTCVNDTSNLPDTVLNFIRSHPLMDKAVNHEHNNPVYYKRDLVFTKLVVDKIRIDILNQEYIVYYVGTNLGRIYKIVQYYRNGESLSKLLDIFEVAPNEAIQVMEISQTRKSLYIGTDHRIKQIDLAMCNRRYDNCFRCVRDPYCGWDKEANTCRPYELDLLQDVANETSDICDSSVLKKKIVVTYGQSVHLGCFVKIPEVLKNEQVTWYHHSKDKGRYEIRYSPTKYIETTERGLVVVSVNEADGGRYDCHLGGSLLCSYNITVDAHRCTPPNKSNDYQKIYSDWCHEFEKYKTAMKSWEKKQAQCSTRQNFSSNQHPNEIFRKPNV

Target ORF information:

RefSeq Version	NM_166178.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster semaphorin 2a, transcript variant C (Sema2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166178.3

ORF Insert Sequence:

ATGAACGGGA TACATTGGCT TCTGTTTGGC AGTTGCCTCA TGCTAATTTC GCAAATAGAG 
GCAGTCACCG AAGAACTTTC ACCAGATCAT GTGCGGGAGT TCAACTGCGG CAAGCTGTAC 
TATCGTACAT TCCATATGAA CGAAGATCGA GATACGCTCT ATGTGGGAGC CATGGATCGC 
GTATTCCGTG TGAACCTGCA GAATATCTCC TCATCCAATT GTAATCGGGA TGTGATCAAC 
TTGGAGCCAA CACGGGATGA TGTGGTTAGC TGCGTCTCCA AAGGCAAAAG TCAGATCTTC 
GACTGCAAGA ACCATGTGCG TGTCATCCAG TCAATGGACC AGGGGGATAG GCTCTATGTA 
TGCGGCACCA ACGCCCACAA TCCCAAGGAT TATGTTATCT ATGCGAATCT AACCCACCTG 
CCGCGCTCGG AATATGTGAT TGGCGTGGGT CTGGGCATTG CCAAGTGCCC CTACGATCCC 
CTCGACAACT CAACTGCGAT TTATGTGGAG AATGGCAATC CGGGTGGTCT GCCCGGTTTG 
TACTCCGGCA CCAATGCGGA GTTCACCAAG GCGGATACGG TTATTTTCCG CACTGATCTG 
TATAATACTT CGGCTAAACG TTTGGAATAT AAATTCAAGA GGACTCTGAA ATACGACTCC 
AAGTGGTTGG ACAAACCAAA CTTTGTCGGC TCCTTTGATA TTGGGGAGTA CGTGTATTTC 
TTTTTCCGTG AAACCGCCGT GGAATACATC AACTGCGGCA AGGCTGTCTA TTCGCGCATC 
GCACGGGTGT GCAAGAAGGA TGTGGGTGGA AAGAATCTGC TGGCCCACAA CTGGGCCACC 
TACCTGAAGG CCAGACTCAA CTGCAGCATC TCCGGCGAAT TTCCGTTCTA TTTCAACGAG 
ATCCAATCGG TCTACCAGCT GCCCTCCGAT AAGAGTCGAT TCTTCGCCAC ATTCACGACG 
AGCACTAATG GCCTGATTGG ATCTGCCGTA TGCAGTTTCC ACATTAACGA GATTCAGGCT 
GCCTTCAATG GCAAATTCAA GGAGCAATCT TCATCGAATT CCGCATGGCT GCCGGTGCTT 
AACTCCCGGG TGCCGGAACC ACGGCCGGGT ACATGTGTCA ACGATACATC AAACCTGCCC 
GATACCGTAC TGAATTTCAT CAGATCCCAT CCACTTATGG ACAAAGCCGT AAATCACGAG 
CACAACAATC CAGTCTATTA TAAAAGGGAT TTGGTCTTCA CCAAGCTCGT CGTTGACAAA 
ATTCGCATTG ACATCCTCAA TCAGGAATAC ATTGTGTACT ATGTGGGCAC CAATCTGGGT 
CGCATTTACA AAATCGTGCA GTACTACCGT AACGGAGAGT CGCTGTCCAA GCTGCTGGAT 
ATCTTCGAGG TGGCTCCAAA CGAGGCCATC CAAGTGATGG AAATCAGCCA GACACGTAAG 
AGCCTCTACA TTGGCACCGA TCATCGCATC AAGCAAATCG ACCTGGCCAT GTGCAATCGC 
CGTTACGACA ACTGCTTCCG CTGCGTCCGT GATCCCTACT GCGGCTGGGA TAAGGAGGCC 
AATACGTGCC GACCGTACGA GCTGGATTTA CTGCAGGATG TGGCCAATGA AACGAGTGAC 
ATTTGCGATT CGAGTGTGCT GAAAAAGAAG ATTGTGGTGA CCTATGGCCA GAGTGTACAT 
CTGGGCTGTT TCGTCAAAAT ACCCGAAGTG CTGAAGAATG AGCAAGTGAC CTGGTATCAT 
CACTCCAAGG ACAAGGGACG CTACGAGATT CGTTACTCGC CGACCAAATA CATTGAGACC 
ACCGAACGTG GCCTGGTTGT GGTTTCCGTG AACGAAGCCG ATGGTGGTCG GTACGATTGC 
CATTTGGGCG GCTCGCTTTT GTGCAGCTAC AACATTACGG TGGATGCCCA CAGATGCACT 
CCGCCGAACA AGAGTAATGA CTATCAGAAA ATCTACTCGG ACTGGTGCCA CGAGTTCGAG 
AAATACAAAA CAGCAATGAA GTCCTGGGAA AAGAAGCAAG CGCAATGCTC GACACGGCAG 
AACTTCAGCA GCAATCAGCA TCCGAATGAG ATTTTCCGTA AGCCCAATGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema2a ( NM_134303.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_599130.1 Drosophila melanogaster semaphorin 2a, transcript variant B (Sema2a), mRNA.

Price & Availability↑

CloneID	OFa10231
Clone ID Related Accession (Same CDS sequence)	NM_134303.4 , NM_058159.5 , NM_206134.2
Accession Version	NM_134303.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	semaphorin 2a, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_134303.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGCTTC TACAGCTATC GCCGCTCCTC GCACTCCTGC TACTCCTCTG CAGTAGTGTG 
AGCGAGACAG CTGCGGACTA CGAGAACACC TGGAACTTCT ACTACGAGCG TCCCTGTTGC 
ACTGGAAACG ATCAGGGGAA CAACAATTAC GGGAAACACG GCGCAGATCA TGTGCGGGAG 
TTCAACTGCG GCAAGCTGTA CTATCGTACA TTCCATATGA ACGAAGATCG AGATACGCTC 
TATGTGGGAG CCATGGATCG CGTATTCCGT GTGAACCTGC AGAATATCTC CTCATCCAAT 
TGTAATCGGG ATGTGATCAA CTTGGAGCCA ACACGGGATG ATGTGGTTAG CTGCGTCTCC 
AAAGGCAAAA GTCAGATCTT CGACTGCAAG AACCATGTGC GTGTCATCCA GTCAATGGAC 
CAGGGGGATA GGCTCTATGT ATGCGGCACC AACGCCCACA ATCCCAAGGA TTATGTTATC 
TATGCGAATC TAACCCACCT GCCGCGCTCG GAATATGTGA TTGGCGTGGG TCTGGGCATT 
GCCAAGTGCC CCTACGATCC CCTCGACAAC TCAACTGCGA TTTATGTGGA GAATGGCAAT 
CCGGGTGGTC TGCCCGGTTT GTACTCCGGC ACCAATGCGG AGTTCACCAA GGCGGATACG 
GTTATTTTCC GCACTGATCT GTATAATACT TCGGCTAAAC GTTTGGAATA TAAATTCAAG 
AGGACTCTGA AATACGACTC CAAGTGGTTG GACAAACCAA ACTTTGTCGG CTCCTTTGAT 
ATTGGGGAGT ACGTGTATTT CTTTTTCCGT GAAACCGCCG TGGAATACAT CAACTGCGGC 
AAGGCTGTCT ATTCGCGCAT CGCACGGGTG TGCAAGAAGG ATGTGGGTGG AAAGAATCTG 
CTGGCCCACA ACTGGGCCAC CTACCTGAAG GCCAGACTCA ACTGCAGCAT CTCCGGCGAA 
TTTCCGTTCT ATTTCAACGA GATCCAATCG GTCTACCAGC TGCCCTCCGA TAAGAGTCGA 
TTCTTCGCCA CATTCACGAC GAGCACTAAT GGCCTGATTG GATCTGCCGT ATGCAGTTTC 
CACATTAACG AGATTCAGGC TGCCTTCAAT GGCAAATTCA AGGAGCAATC TTCATCGAAT 
TCCGCATGGC TGCCGGTGCT TAACTCCCGG GTGCCGGAAC CACGGCCGGG TACATGTGTC 
AACGATACAT CAAACCTGCC CGATACCGTA CTGAATTTCA TCAGATCCCA TCCACTTATG 
GACAAAGCCG TAAATCACGA GCACAACAAT CCAGTCTATT ATAAAAGGGA TTTGGTCTTC 
ACCAAGCTCG TCGTTGACAA AATTCGCATT GACATCCTCA ATCAGGAATA CATTGTGTAC 
TATGTGGGCA CCAATCTGGG TCGCATTTAC AAAATCGTGC AGTACTACCG TAACGGAGAG 
TCGCTGTCCA AGCTGCTGGA TATCTTCGAG GTGGCTCCAA ACGAGGCCAT CCAAGTGATG 
GAAATCAGCC AGACACGTAA GAGCCTCTAC ATTGGCACCG ATCATCGCAT CAAGCAAATC 
GACCTGGCCA TGTGCAATCG CCGTTACGAC AACTGCTTCC GCTGCGTCCG TGATCCCTAC 
TGCGGCTGGG ATAAGGAGGC CAATACGTGC CGACCGTACG AGCTGGATTT ACTGCAGGAT 
GTGGCCAATG AAACGAGTGA CATTTGCGAT TCGAGTGTGC TGAAAAAGAA GATTGTGGTG 
ACCTATGGCC AGAGTGTACA TCTGGGCTGT TTCGTCAAAA TACCCGAAGT GCTGAAGAAT 
GAGCAAGTGA CCTGGTATCA TCACTCCAAG GACAAGGGAC GCTACGAGAT TCGTTACTCG 
CCGACCAAAT ACATTGAGAC CACCGAACGT GGCCTGGTTG TGGTTTCCGT GAACGAAGCC 
GATGGTGGTC GGTACGATTG CCATTTGGGC GGCTCGCTTT TGTGCAGCTA CAACATTACG 
GTGGATGCCC ACAGATGCAC TCCGCCGAAC AAGAGTAATG ACTATCAGAA AATCTACTCG 
GACTGGTGCC ACGAGTTCGA GAAATACAAA ACAGCAATGA AGTCCTGGGA AAAGAAGCAA 
GCGCAATGCT CGACACGGCA GAACTTCAGC AGCAATCAGC ATCCGAATGA GATTTTCCGT 
AAGCCCAATG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_599130.1
CDS	279..2453
Translation	MSLLQLSPLLALLLLLCSSVSETAADYENTWNFYYERPCCTGNDQGNNNYGKHGADHVREFNCGKLYYRTFHMNEDRDTLYVGAMDRVFRVNLQNISSSNCNRDVINLEPTRDDVVSCVSKGKSQIFDCKNHVRVIQSMDQGDRLYVCGTNAHNPKDYVIYANLTHLPRSEYVIGVGLGIAKCPYDPLDNSTAIYVENGNPGGLPGLYSGTNAEFTKADTVIFRTDLYNTSAKRLEYKFKRTLKYDSKWLDKPNFVGSFDIGEYVYFFFRETAVEYINCGKAVYSRIARVCKKDVGGKNLLAHNWATYLKARLNCSISGEFPFYFNEIQSVYQLPSDKSRFFATFTTSTNGLIGSAVCSFHINEIQAAFNGKFKEQSSSNSAWLPVLNSRVPEPRPGTCVNDTSNLPDTVLNFIRSHPLMDKAVNHEHNNPVYYKRDLVFTKLVVDKIRIDILNQEYIVYYVGTNLGRIYKIVQYYRNGESLSKLLDIFEVAPNEAIQVMEISQTRKSLYIGTDHRIKQIDLAMCNRRYDNCFRCVRDPYCGWDKEANTCRPYELDLLQDVANETSDICDSSVLKKKIVVTYGQSVHLGCFVKIPEVLKNEQVTWYHHSKDKGRYEIRYSPTKYIETTERGLVVVSVNEADGGRYDCHLGGSLLCSYNITVDAHRCTPPNKSNDYQKIYSDWCHEFEKYKTAMKSWEKKQAQCSTRQNFSSNQHPNEIFRKPNV

Target ORF information:

RefSeq Version	NM_134303.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster semaphorin 2a, transcript variant B (Sema2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134303.4

ORF Insert Sequence:

ATGTCGCTTC TACAGCTATC GCCGCTCCTC GCACTCCTGC TACTCCTCTG CAGTAGTGTG 
AGCGAGACAG CTGCGGACTA CGAGAACACC TGGAACTTCT ACTACGAGCG TCCCTGTTGC 
ACTGGAAACG ATCAGGGGAA CAACAATTAC GGGAAACACG GCGCAGATCA TGTGCGGGAG 
TTCAACTGCG GCAAGCTGTA CTATCGTACA TTCCATATGA ACGAAGATCG AGATACGCTC 
TATGTGGGAG CCATGGATCG CGTATTCCGT GTGAACCTGC AGAATATCTC CTCATCCAAT 
TGTAATCGGG ATGTGATCAA CTTGGAGCCA ACACGGGATG ATGTGGTTAG CTGCGTCTCC 
AAAGGCAAAA GTCAGATCTT CGACTGCAAG AACCATGTGC GTGTCATCCA GTCAATGGAC 
CAGGGGGATA GGCTCTATGT ATGCGGCACC AACGCCCACA ATCCCAAGGA TTATGTTATC 
TATGCGAATC TAACCCACCT GCCGCGCTCG GAATATGTGA TTGGCGTGGG TCTGGGCATT 
GCCAAGTGCC CCTACGATCC CCTCGACAAC TCAACTGCGA TTTATGTGGA GAATGGCAAT 
CCGGGTGGTC TGCCCGGTTT GTACTCCGGC ACCAATGCGG AGTTCACCAA GGCGGATACG 
GTTATTTTCC GCACTGATCT GTATAATACT TCGGCTAAAC GTTTGGAATA TAAATTCAAG 
AGGACTCTGA AATACGACTC CAAGTGGTTG GACAAACCAA ACTTTGTCGG CTCCTTTGAT 
ATTGGGGAGT ACGTGTATTT CTTTTTCCGT GAAACCGCCG TGGAATACAT CAACTGCGGC 
AAGGCTGTCT ATTCGCGCAT CGCACGGGTG TGCAAGAAGG ATGTGGGTGG AAAGAATCTG 
CTGGCCCACA ACTGGGCCAC CTACCTGAAG GCCAGACTCA ACTGCAGCAT CTCCGGCGAA 
TTTCCGTTCT ATTTCAACGA GATCCAATCG GTCTACCAGC TGCCCTCCGA TAAGAGTCGA 
TTCTTCGCCA CATTCACGAC GAGCACTAAT GGCCTGATTG GATCTGCCGT ATGCAGTTTC 
CACATTAACG AGATTCAGGC TGCCTTCAAT GGCAAATTCA AGGAGCAATC TTCATCGAAT 
TCCGCATGGC TGCCGGTGCT TAACTCCCGG GTGCCGGAAC CACGGCCGGG TACATGTGTC 
AACGATACAT CAAACCTGCC CGATACCGTA CTGAATTTCA TCAGATCCCA TCCACTTATG 
GACAAAGCCG TAAATCACGA GCACAACAAT CCAGTCTATT ATAAAAGGGA TTTGGTCTTC 
ACCAAGCTCG TCGTTGACAA AATTCGCATT GACATCCTCA ATCAGGAATA CATTGTGTAC 
TATGTGGGCA CCAATCTGGG TCGCATTTAC AAAATCGTGC AGTACTACCG TAACGGAGAG 
TCGCTGTCCA AGCTGCTGGA TATCTTCGAG GTGGCTCCAA ACGAGGCCAT CCAAGTGATG 
GAAATCAGCC AGACACGTAA GAGCCTCTAC ATTGGCACCG ATCATCGCAT CAAGCAAATC 
GACCTGGCCA TGTGCAATCG CCGTTACGAC AACTGCTTCC GCTGCGTCCG TGATCCCTAC 
TGCGGCTGGG ATAAGGAGGC CAATACGTGC CGACCGTACG AGCTGGATTT ACTGCAGGAT 
GTGGCCAATG AAACGAGTGA CATTTGCGAT TCGAGTGTGC TGAAAAAGAA GATTGTGGTG 
ACCTATGGCC AGAGTGTACA TCTGGGCTGT TTCGTCAAAA TACCCGAAGT GCTGAAGAAT 
GAGCAAGTGA CCTGGTATCA TCACTCCAAG GACAAGGGAC GCTACGAGAT TCGTTACTCG 
CCGACCAAAT ACATTGAGAC CACCGAACGT GGCCTGGTTG TGGTTTCCGT GAACGAAGCC 
GATGGTGGTC GGTACGATTG CCATTTGGGC GGCTCGCTTT TGTGCAGCTA CAACATTACG 
GTGGATGCCC ACAGATGCAC TCCGCCGAAC AAGAGTAATG ACTATCAGAA AATCTACTCG 
GACTGGTGCC ACGAGTTCGA GAAATACAAA ACAGCAATGA AGTCCTGGGA AAAGAAGCAA 
GCGCAATGCT CGACACGGCA GAACTTCAGC AGCAATCAGC ATCCGAATGA GATTTTCCGT 
AAGCCCAATG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema2a ( NM_058159.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477507.1 Drosophila melanogaster semaphorin 2a, transcript variant A (Sema2a), mRNA.

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CloneID	OFa10231
Clone ID Related Accession (Same CDS sequence)	NM_134303.4 , NM_058159.5 , NM_206134.2
Accession Version	NM_058159.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	semaphorin 2a, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_058159.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGCTTC TACAGCTATC GCCGCTCCTC GCACTCCTGC TACTCCTCTG CAGTAGTGTG 
AGCGAGACAG CTGCGGACTA CGAGAACACC TGGAACTTCT ACTACGAGCG TCCCTGTTGC 
ACTGGAAACG ATCAGGGGAA CAACAATTAC GGGAAACACG GCGCAGATCA TGTGCGGGAG 
TTCAACTGCG GCAAGCTGTA CTATCGTACA TTCCATATGA ACGAAGATCG AGATACGCTC 
TATGTGGGAG CCATGGATCG CGTATTCCGT GTGAACCTGC AGAATATCTC CTCATCCAAT 
TGTAATCGGG ATGTGATCAA CTTGGAGCCA ACACGGGATG ATGTGGTTAG CTGCGTCTCC 
AAAGGCAAAA GTCAGATCTT CGACTGCAAG AACCATGTGC GTGTCATCCA GTCAATGGAC 
CAGGGGGATA GGCTCTATGT ATGCGGCACC AACGCCCACA ATCCCAAGGA TTATGTTATC 
TATGCGAATC TAACCCACCT GCCGCGCTCG GAATATGTGA TTGGCGTGGG TCTGGGCATT 
GCCAAGTGCC CCTACGATCC CCTCGACAAC TCAACTGCGA TTTATGTGGA GAATGGCAAT 
CCGGGTGGTC TGCCCGGTTT GTACTCCGGC ACCAATGCGG AGTTCACCAA GGCGGATACG 
GTTATTTTCC GCACTGATCT GTATAATACT TCGGCTAAAC GTTTGGAATA TAAATTCAAG 
AGGACTCTGA AATACGACTC CAAGTGGTTG GACAAACCAA ACTTTGTCGG CTCCTTTGAT 
ATTGGGGAGT ACGTGTATTT CTTTTTCCGT GAAACCGCCG TGGAATACAT CAACTGCGGC 
AAGGCTGTCT ATTCGCGCAT CGCACGGGTG TGCAAGAAGG ATGTGGGTGG AAAGAATCTG 
CTGGCCCACA ACTGGGCCAC CTACCTGAAG GCCAGACTCA ACTGCAGCAT CTCCGGCGAA 
TTTCCGTTCT ATTTCAACGA GATCCAATCG GTCTACCAGC TGCCCTCCGA TAAGAGTCGA 
TTCTTCGCCA CATTCACGAC GAGCACTAAT GGCCTGATTG GATCTGCCGT ATGCAGTTTC 
CACATTAACG AGATTCAGGC TGCCTTCAAT GGCAAATTCA AGGAGCAATC TTCATCGAAT 
TCCGCATGGC TGCCGGTGCT TAACTCCCGG GTGCCGGAAC CACGGCCGGG TACATGTGTC 
AACGATACAT CAAACCTGCC CGATACCGTA CTGAATTTCA TCAGATCCCA TCCACTTATG 
GACAAAGCCG TAAATCACGA GCACAACAAT CCAGTCTATT ATAAAAGGGA TTTGGTCTTC 
ACCAAGCTCG TCGTTGACAA AATTCGCATT GACATCCTCA ATCAGGAATA CATTGTGTAC 
TATGTGGGCA CCAATCTGGG TCGCATTTAC AAAATCGTGC AGTACTACCG TAACGGAGAG 
TCGCTGTCCA AGCTGCTGGA TATCTTCGAG GTGGCTCCAA ACGAGGCCAT CCAAGTGATG 
GAAATCAGCC AGACACGTAA GAGCCTCTAC ATTGGCACCG ATCATCGCAT CAAGCAAATC 
GACCTGGCCA TGTGCAATCG CCGTTACGAC AACTGCTTCC GCTGCGTCCG TGATCCCTAC 
TGCGGCTGGG ATAAGGAGGC CAATACGTGC CGACCGTACG AGCTGGATTT ACTGCAGGAT 
GTGGCCAATG AAACGAGTGA CATTTGCGAT TCGAGTGTGC TGAAAAAGAA GATTGTGGTG 
ACCTATGGCC AGAGTGTACA TCTGGGCTGT TTCGTCAAAA TACCCGAAGT GCTGAAGAAT 
GAGCAAGTGA CCTGGTATCA TCACTCCAAG GACAAGGGAC GCTACGAGAT TCGTTACTCG 
CCGACCAAAT ACATTGAGAC CACCGAACGT GGCCTGGTTG TGGTTTCCGT GAACGAAGCC 
GATGGTGGTC GGTACGATTG CCATTTGGGC GGCTCGCTTT TGTGCAGCTA CAACATTACG 
GTGGATGCCC ACAGATGCAC TCCGCCGAAC AAGAGTAATG ACTATCAGAA AATCTACTCG 
GACTGGTGCC ACGAGTTCGA GAAATACAAA ACAGCAATGA AGTCCTGGGA AAAGAAGCAA 
GCGCAATGCT CGACACGGCA GAACTTCAGC AGCAATCAGC ATCCGAATGA GATTTTCCGT 
AAGCCCAATG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477507.1
CDS	378..2552
Translation	MSLLQLSPLLALLLLLCSSVSETAADYENTWNFYYERPCCTGNDQGNNNYGKHGADHVREFNCGKLYYRTFHMNEDRDTLYVGAMDRVFRVNLQNISSSNCNRDVINLEPTRDDVVSCVSKGKSQIFDCKNHVRVIQSMDQGDRLYVCGTNAHNPKDYVIYANLTHLPRSEYVIGVGLGIAKCPYDPLDNSTAIYVENGNPGGLPGLYSGTNAEFTKADTVIFRTDLYNTSAKRLEYKFKRTLKYDSKWLDKPNFVGSFDIGEYVYFFFRETAVEYINCGKAVYSRIARVCKKDVGGKNLLAHNWATYLKARLNCSISGEFPFYFNEIQSVYQLPSDKSRFFATFTTSTNGLIGSAVCSFHINEIQAAFNGKFKEQSSSNSAWLPVLNSRVPEPRPGTCVNDTSNLPDTVLNFIRSHPLMDKAVNHEHNNPVYYKRDLVFTKLVVDKIRIDILNQEYIVYYVGTNLGRIYKIVQYYRNGESLSKLLDIFEVAPNEAIQVMEISQTRKSLYIGTDHRIKQIDLAMCNRRYDNCFRCVRDPYCGWDKEANTCRPYELDLLQDVANETSDICDSSVLKKKIVVTYGQSVHLGCFVKIPEVLKNEQVTWYHHSKDKGRYEIRYSPTKYIETTERGLVVVSVNEADGGRYDCHLGGSLLCSYNITVDAHRCTPPNKSNDYQKIYSDWCHEFEKYKTAMKSWEKKQAQCSTRQNFSSNQHPNEIFRKPNV

Target ORF information:

RefSeq Version	NM_058159.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster semaphorin 2a, transcript variant A (Sema2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058159.5

ORF Insert Sequence:

ATGTCGCTTC TACAGCTATC GCCGCTCCTC GCACTCCTGC TACTCCTCTG CAGTAGTGTG 
AGCGAGACAG CTGCGGACTA CGAGAACACC TGGAACTTCT ACTACGAGCG TCCCTGTTGC 
ACTGGAAACG ATCAGGGGAA CAACAATTAC GGGAAACACG GCGCAGATCA TGTGCGGGAG 
TTCAACTGCG GCAAGCTGTA CTATCGTACA TTCCATATGA ACGAAGATCG AGATACGCTC 
TATGTGGGAG CCATGGATCG CGTATTCCGT GTGAACCTGC AGAATATCTC CTCATCCAAT 
TGTAATCGGG ATGTGATCAA CTTGGAGCCA ACACGGGATG ATGTGGTTAG CTGCGTCTCC 
AAAGGCAAAA GTCAGATCTT CGACTGCAAG AACCATGTGC GTGTCATCCA GTCAATGGAC 
CAGGGGGATA GGCTCTATGT ATGCGGCACC AACGCCCACA ATCCCAAGGA TTATGTTATC 
TATGCGAATC TAACCCACCT GCCGCGCTCG GAATATGTGA TTGGCGTGGG TCTGGGCATT 
GCCAAGTGCC CCTACGATCC CCTCGACAAC TCAACTGCGA TTTATGTGGA GAATGGCAAT 
CCGGGTGGTC TGCCCGGTTT GTACTCCGGC ACCAATGCGG AGTTCACCAA GGCGGATACG 
GTTATTTTCC GCACTGATCT GTATAATACT TCGGCTAAAC GTTTGGAATA TAAATTCAAG 
AGGACTCTGA AATACGACTC CAAGTGGTTG GACAAACCAA ACTTTGTCGG CTCCTTTGAT 
ATTGGGGAGT ACGTGTATTT CTTTTTCCGT GAAACCGCCG TGGAATACAT CAACTGCGGC 
AAGGCTGTCT ATTCGCGCAT CGCACGGGTG TGCAAGAAGG ATGTGGGTGG AAAGAATCTG 
CTGGCCCACA ACTGGGCCAC CTACCTGAAG GCCAGACTCA ACTGCAGCAT CTCCGGCGAA 
TTTCCGTTCT ATTTCAACGA GATCCAATCG GTCTACCAGC TGCCCTCCGA TAAGAGTCGA 
TTCTTCGCCA CATTCACGAC GAGCACTAAT GGCCTGATTG GATCTGCCGT ATGCAGTTTC 
CACATTAACG AGATTCAGGC TGCCTTCAAT GGCAAATTCA AGGAGCAATC TTCATCGAAT 
TCCGCATGGC TGCCGGTGCT TAACTCCCGG GTGCCGGAAC CACGGCCGGG TACATGTGTC 
AACGATACAT CAAACCTGCC CGATACCGTA CTGAATTTCA TCAGATCCCA TCCACTTATG 
GACAAAGCCG TAAATCACGA GCACAACAAT CCAGTCTATT ATAAAAGGGA TTTGGTCTTC 
ACCAAGCTCG TCGTTGACAA AATTCGCATT GACATCCTCA ATCAGGAATA CATTGTGTAC 
TATGTGGGCA CCAATCTGGG TCGCATTTAC AAAATCGTGC AGTACTACCG TAACGGAGAG 
TCGCTGTCCA AGCTGCTGGA TATCTTCGAG GTGGCTCCAA ACGAGGCCAT CCAAGTGATG 
GAAATCAGCC AGACACGTAA GAGCCTCTAC ATTGGCACCG ATCATCGCAT CAAGCAAATC 
GACCTGGCCA TGTGCAATCG CCGTTACGAC AACTGCTTCC GCTGCGTCCG TGATCCCTAC 
TGCGGCTGGG ATAAGGAGGC CAATACGTGC CGACCGTACG AGCTGGATTT ACTGCAGGAT 
GTGGCCAATG AAACGAGTGA CATTTGCGAT TCGAGTGTGC TGAAAAAGAA GATTGTGGTG 
ACCTATGGCC AGAGTGTACA TCTGGGCTGT TTCGTCAAAA TACCCGAAGT GCTGAAGAAT 
GAGCAAGTGA CCTGGTATCA TCACTCCAAG GACAAGGGAC GCTACGAGAT TCGTTACTCG 
CCGACCAAAT ACATTGAGAC CACCGAACGT GGCCTGGTTG TGGTTTCCGT GAACGAAGCC 
GATGGTGGTC GGTACGATTG CCATTTGGGC GGCTCGCTTT TGTGCAGCTA CAACATTACG 
GTGGATGCCC ACAGATGCAC TCCGCCGAAC AAGAGTAATG ACTATCAGAA AATCTACTCG 
GACTGGTGCC ACGAGTTCGA GAAATACAAA ACAGCAATGA AGTCCTGGGA AAAGAAGCAA 
GCGCAATGCT CGACACGGCA GAACTTCAGC AGCAATCAGC ATCCGAATGA GATTTTCCGT 
AAGCCCAATG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema2a ( NM_206134.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995856.2 Drosophila melanogaster semaphorin 2a, transcript variant E (Sema2a), mRNA.

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CloneID	OFa10231
Clone ID Related Accession (Same CDS sequence)	NM_134303.4 , NM_058159.5 , NM_206134.2
Accession Version	NM_206134.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	semaphorin 2a, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_206134.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGCTTC TACAGCTATC GCCGCTCCTC GCACTCCTGC TACTCCTCTG CAGTAGTGTG 
AGCGAGACAG CTGCGGACTA CGAGAACACC TGGAACTTCT ACTACGAGCG TCCCTGTTGC 
ACTGGAAACG ATCAGGGGAA CAACAATTAC GGGAAACACG GCGCAGATCA TGTGCGGGAG 
TTCAACTGCG GCAAGCTGTA CTATCGTACA TTCCATATGA ACGAAGATCG AGATACGCTC 
TATGTGGGAG CCATGGATCG CGTATTCCGT GTGAACCTGC AGAATATCTC CTCATCCAAT 
TGTAATCGGG ATGTGATCAA CTTGGAGCCA ACACGGGATG ATGTGGTTAG CTGCGTCTCC 
AAAGGCAAAA GTCAGATCTT CGACTGCAAG AACCATGTGC GTGTCATCCA GTCAATGGAC 
CAGGGGGATA GGCTCTATGT ATGCGGCACC AACGCCCACA ATCCCAAGGA TTATGTTATC 
TATGCGAATC TAACCCACCT GCCGCGCTCG GAATATGTGA TTGGCGTGGG TCTGGGCATT 
GCCAAGTGCC CCTACGATCC CCTCGACAAC TCAACTGCGA TTTATGTGGA GAATGGCAAT 
CCGGGTGGTC TGCCCGGTTT GTACTCCGGC ACCAATGCGG AGTTCACCAA GGCGGATACG 
GTTATTTTCC GCACTGATCT GTATAATACT TCGGCTAAAC GTTTGGAATA TAAATTCAAG 
AGGACTCTGA AATACGACTC CAAGTGGTTG GACAAACCAA ACTTTGTCGG CTCCTTTGAT 
ATTGGGGAGT ACGTGTATTT CTTTTTCCGT GAAACCGCCG TGGAATACAT CAACTGCGGC 
AAGGCTGTCT ATTCGCGCAT CGCACGGGTG TGCAAGAAGG ATGTGGGTGG AAAGAATCTG 
CTGGCCCACA ACTGGGCCAC CTACCTGAAG GCCAGACTCA ACTGCAGCAT CTCCGGCGAA 
TTTCCGTTCT ATTTCAACGA GATCCAATCG GTCTACCAGC TGCCCTCCGA TAAGAGTCGA 
TTCTTCGCCA CATTCACGAC GAGCACTAAT GGCCTGATTG GATCTGCCGT ATGCAGTTTC 
CACATTAACG AGATTCAGGC TGCCTTCAAT GGCAAATTCA AGGAGCAATC TTCATCGAAT 
TCCGCATGGC TGCCGGTGCT TAACTCCCGG GTGCCGGAAC CACGGCCGGG TACATGTGTC 
AACGATACAT CAAACCTGCC CGATACCGTA CTGAATTTCA TCAGATCCCA TCCACTTATG 
GACAAAGCCG TAAATCACGA GCACAACAAT CCAGTCTATT ATAAAAGGGA TTTGGTCTTC 
ACCAAGCTCG TCGTTGACAA AATTCGCATT GACATCCTCA ATCAGGAATA CATTGTGTAC 
TATGTGGGCA CCAATCTGGG TCGCATTTAC AAAATCGTGC AGTACTACCG TAACGGAGAG 
TCGCTGTCCA AGCTGCTGGA TATCTTCGAG GTGGCTCCAA ACGAGGCCAT CCAAGTGATG 
GAAATCAGCC AGACACGTAA GAGCCTCTAC ATTGGCACCG ATCATCGCAT CAAGCAAATC 
GACCTGGCCA TGTGCAATCG CCGTTACGAC AACTGCTTCC GCTGCGTCCG TGATCCCTAC 
TGCGGCTGGG ATAAGGAGGC CAATACGTGC CGACCGTACG AGCTGGATTT ACTGCAGGAT 
GTGGCCAATG AAACGAGTGA CATTTGCGAT TCGAGTGTGC TGAAAAAGAA GATTGTGGTG 
ACCTATGGCC AGAGTGTACA TCTGGGCTGT TTCGTCAAAA TACCCGAAGT GCTGAAGAAT 
GAGCAAGTGA CCTGGTATCA TCACTCCAAG GACAAGGGAC GCTACGAGAT TCGTTACTCG 
CCGACCAAAT ACATTGAGAC CACCGAACGT GGCCTGGTTG TGGTTTCCGT GAACGAAGCC 
GATGGTGGTC GGTACGATTG CCATTTGGGC GGCTCGCTTT TGTGCAGCTA CAACATTACG 
GTGGATGCCC ACAGATGCAC TCCGCCGAAC AAGAGTAATG ACTATCAGAA AATCTACTCG 
GACTGGTGCC ACGAGTTCGA GAAATACAAA ACAGCAATGA AGTCCTGGGA AAAGAAGCAA 
GCGCAATGCT CGACACGGCA GAACTTCAGC AGCAATCAGC ATCCGAATGA GATTTTCCGT 
AAGCCCAATG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995856.2
CDS	238..2412
Translation	MSLLQLSPLLALLLLLCSSVSETAADYENTWNFYYERPCCTGNDQGNNNYGKHGADHVREFNCGKLYYRTFHMNEDRDTLYVGAMDRVFRVNLQNISSSNCNRDVINLEPTRDDVVSCVSKGKSQIFDCKNHVRVIQSMDQGDRLYVCGTNAHNPKDYVIYANLTHLPRSEYVIGVGLGIAKCPYDPLDNSTAIYVENGNPGGLPGLYSGTNAEFTKADTVIFRTDLYNTSAKRLEYKFKRTLKYDSKWLDKPNFVGSFDIGEYVYFFFRETAVEYINCGKAVYSRIARVCKKDVGGKNLLAHNWATYLKARLNCSISGEFPFYFNEIQSVYQLPSDKSRFFATFTTSTNGLIGSAVCSFHINEIQAAFNGKFKEQSSSNSAWLPVLNSRVPEPRPGTCVNDTSNLPDTVLNFIRSHPLMDKAVNHEHNNPVYYKRDLVFTKLVVDKIRIDILNQEYIVYYVGTNLGRIYKIVQYYRNGESLSKLLDIFEVAPNEAIQVMEISQTRKSLYIGTDHRIKQIDLAMCNRRYDNCFRCVRDPYCGWDKEANTCRPYELDLLQDVANETSDICDSSVLKKKIVVTYGQSVHLGCFVKIPEVLKNEQVTWYHHSKDKGRYEIRYSPTKYIETTERGLVVVSVNEADGGRYDCHLGGSLLCSYNITVDAHRCTPPNKSNDYQKIYSDWCHEFEKYKTAMKSWEKKQAQCSTRQNFSSNQHPNEIFRKPNV

Target ORF information:

RefSeq Version	NM_206134.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster semaphorin 2a, transcript variant E (Sema2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206134.2

ORF Insert Sequence:

ATGTCGCTTC TACAGCTATC GCCGCTCCTC GCACTCCTGC TACTCCTCTG CAGTAGTGTG 
AGCGAGACAG CTGCGGACTA CGAGAACACC TGGAACTTCT ACTACGAGCG TCCCTGTTGC 
ACTGGAAACG ATCAGGGGAA CAACAATTAC GGGAAACACG GCGCAGATCA TGTGCGGGAG 
TTCAACTGCG GCAAGCTGTA CTATCGTACA TTCCATATGA ACGAAGATCG AGATACGCTC 
TATGTGGGAG CCATGGATCG CGTATTCCGT GTGAACCTGC AGAATATCTC CTCATCCAAT 
TGTAATCGGG ATGTGATCAA CTTGGAGCCA ACACGGGATG ATGTGGTTAG CTGCGTCTCC 
AAAGGCAAAA GTCAGATCTT CGACTGCAAG AACCATGTGC GTGTCATCCA GTCAATGGAC 
CAGGGGGATA GGCTCTATGT ATGCGGCACC AACGCCCACA ATCCCAAGGA TTATGTTATC 
TATGCGAATC TAACCCACCT GCCGCGCTCG GAATATGTGA TTGGCGTGGG TCTGGGCATT 
GCCAAGTGCC CCTACGATCC CCTCGACAAC TCAACTGCGA TTTATGTGGA GAATGGCAAT 
CCGGGTGGTC TGCCCGGTTT GTACTCCGGC ACCAATGCGG AGTTCACCAA GGCGGATACG 
GTTATTTTCC GCACTGATCT GTATAATACT TCGGCTAAAC GTTTGGAATA TAAATTCAAG 
AGGACTCTGA AATACGACTC CAAGTGGTTG GACAAACCAA ACTTTGTCGG CTCCTTTGAT 
ATTGGGGAGT ACGTGTATTT CTTTTTCCGT GAAACCGCCG TGGAATACAT CAACTGCGGC 
AAGGCTGTCT ATTCGCGCAT CGCACGGGTG TGCAAGAAGG ATGTGGGTGG AAAGAATCTG 
CTGGCCCACA ACTGGGCCAC CTACCTGAAG GCCAGACTCA ACTGCAGCAT CTCCGGCGAA 
TTTCCGTTCT ATTTCAACGA GATCCAATCG GTCTACCAGC TGCCCTCCGA TAAGAGTCGA 
TTCTTCGCCA CATTCACGAC GAGCACTAAT GGCCTGATTG GATCTGCCGT ATGCAGTTTC 
CACATTAACG AGATTCAGGC TGCCTTCAAT GGCAAATTCA AGGAGCAATC TTCATCGAAT 
TCCGCATGGC TGCCGGTGCT TAACTCCCGG GTGCCGGAAC CACGGCCGGG TACATGTGTC 
AACGATACAT CAAACCTGCC CGATACCGTA CTGAATTTCA TCAGATCCCA TCCACTTATG 
GACAAAGCCG TAAATCACGA GCACAACAAT CCAGTCTATT ATAAAAGGGA TTTGGTCTTC 
ACCAAGCTCG TCGTTGACAA AATTCGCATT GACATCCTCA ATCAGGAATA CATTGTGTAC 
TATGTGGGCA CCAATCTGGG TCGCATTTAC AAAATCGTGC AGTACTACCG TAACGGAGAG 
TCGCTGTCCA AGCTGCTGGA TATCTTCGAG GTGGCTCCAA ACGAGGCCAT CCAAGTGATG 
GAAATCAGCC AGACACGTAA GAGCCTCTAC ATTGGCACCG ATCATCGCAT CAAGCAAATC 
GACCTGGCCA TGTGCAATCG CCGTTACGAC AACTGCTTCC GCTGCGTCCG TGATCCCTAC 
TGCGGCTGGG ATAAGGAGGC CAATACGTGC CGACCGTACG AGCTGGATTT ACTGCAGGAT 
GTGGCCAATG AAACGAGTGA CATTTGCGAT TCGAGTGTGC TGAAAAAGAA GATTGTGGTG 
ACCTATGGCC AGAGTGTACA TCTGGGCTGT TTCGTCAAAA TACCCGAAGT GCTGAAGAAT 
GAGCAAGTGA CCTGGTATCA TCACTCCAAG GACAAGGGAC GCTACGAGAT TCGTTACTCG 
CCGACCAAAT ACATTGAGAC CACCGAACGT GGCCTGGTTG TGGTTTCCGT GAACGAAGCC 
GATGGTGGTC GGTACGATTG CCATTTGGGC GGCTCGCTTT TGTGCAGCTA CAACATTACG 
GTGGATGCCC ACAGATGCAC TCCGCCGAAC AAGAGTAATG ACTATCAGAA AATCTACTCG 
GACTGGTGCC ACGAGTTCGA GAAATACAAA ACAGCAATGA AGTCCTGGGA AAAGAAGCAA 
GCGCAATGCT CGACACGGCA GAACTTCAGC AGCAATCAGC ATCCGAATGA GATTTTCCGT 
AAGCCCAATG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Sema2a cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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