clu cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	clu
Entrez Gene ID	36793
Full Name	clueless
Synonyms	CG8443,Clu,Dmel\CG8443,anon-WO0118547.143
General protein information	Preferred Names clueless Names CG8443 gene product from transcript CG8443-RA CG8443-PA CG8443-PB clu-PA clu-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 52F5-52F7

mRNA and Protein(s)

mRNA	Protein	Name
NM_137251.3	NP_611095.1	clueless, isoform A
NM_001274092.1	NP_001261021.1	clueless, isoform B

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Cluh	NP_001074627.1
Gallus gallus (chicken)	KIAA0664	XP_415920.4
Schizosaccharomyces pombe (fission yeast)	SPBC530.06c	NP_595319.2
Canis lupus familiaris (dog)	KIAA0664	XP_548323.2
Danio rerio (zebrafish)	cluha	NP_001071028.1
Magnaporthe oryzae (rice blast fungus)	MGG_06261	XP_003717331.1
Xenopus tropicalis (tropical clawed frog)	cluh	NP_001072577.1
Homo sapiens (human)	CLUH	NP_056044.3
Pan troglodytes (chimpanzee)	KIAA0664	XP_003952989.1
Rattus norvegicus (Norway rat)	Cluh	XP_006246920.1
Drosophila melanogaster (fruit fly)	clu	NP_611095.1
Anopheles gambiae (African malaria mosquito)	K0664_ANOGA	XP_320668.4
Oryza sativa (rice)	Os02g0717400	NP_001047932.1
	LOC704219	XP_002800295.1
Bos taurus (cattle)	KIAA0664	XP_005220213.1
Caenorhabditis elegans (roundworm)	clu-1	NP_499097.1
Neurospora crassa	NCU00021	XP_957008.2
Arabidopsis thaliana (thale cress)	AT3G52140	NP_001190066.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Clu is upstream of and binds to VCP in vivo and promotes VCP-dependent Marf degradation in vitro Marf accumulates in whole muscle lysates of clu-deficient flies and is destabilized upon Clu overexpression. Thus, Clu is essential for mitochondrial homeostasis and functions in concert with Parkin and VCP for Marf degradation to promote damaged mitochondrial clearance.
Title: Drosophila clueless is involved in Parkin-dependent mitophagy by promoting VCP-mediated Marf degradation.

Clu directly modulates mitochondrial function, and that Clu's function contributes to the PINK1-Park pathway of mitochondrial quality control.
Title: Clueless, a protein required for mitochondrial function, interacts with the PINK1-Parkin complex in Drosophila.

Clueless (clu) is a novel regulator of aPKC activity.
Title: Clueless regulates aPKC activity and promotes self-renewal cell fate in Drosophila lgl mutant larval brains.

The results suggest Clu functions upstream of electron transport and oxidative phosphorylation, has a role in suppressing oxidative damage in the cell, and that lack of Clu's specific function causes mitochondria to mislocalize.
Title: Drosophila clueless is highly expressed in larval neuroblasts, affects mitochondrial localization and suppresses mitochondrial oxidative damage.

strong clu mutations resemble park mutations in their effects on mitochondrial function and that the two genes interact genetically
Title: Clueless, a conserved Drosophila gene required for mitochondrial subcellular localization, interacts genetically with parkin.

The following clu gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the clu cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa12297	NM_001274092.1 Latest version!	Drosophila melanogaster clueless (clu), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa10180	NM_137251.3 Latest version!	Drosophila melanogaster clueless (clu), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

clu ( NM_001274092.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261021.1 Drosophila melanogaster clueless (clu), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa12297
Clone ID Related Accession (Same CDS sequence)	NM_001274092.1
Accession Version	NM_001274092.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3870bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	clueless, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGCTGG TGCAGGAAAT CCACCAGCTG CTGATGGACC GCGAAGAGAC GTGCCATCGT 
ACATGCTTCT CGCTGCAGCT GGACAACGTG ACGCTCGACA ATTTCGCCGA ACTCAAGTCA 
ATCAATAACC TTGAGCAGGG CTCGACGATC AAAGTGGTAG AGGAGCCGTA CACCATGCGG 
GAGGCCCGTA TTCATGTGCG CCATGTCCGT GATCTTCTCA AAAACCTTGA CCCGGCCGAC 
GCGTACAATG GCATTGACTG TACTTCGCTG ACCTACTTAA ACACGATCAC TCAGGGTGAT 
TTGCTGGACA AGAAGAGGAC GCGACCGGAC TCCGTGGATT GCACTCCGCC GGAGTATGTG 
ACTCCGGGTG TAAGTGATCC GCCAATTCTG CCATTGCATC CAAATGTGAA GAACGCCAAG 
GGACCGCAGG CTCTCAAGGT ACTGACTACA TCCGCCTGGA ACCCGCCACC CGGTCCTCGC 
AAGCTGCATG GTGACCTCAT GTATCTGTAC GTTGTCACCA TGGAGGATAA ACGATTCCAC 
ATCTCAGCCT GTTCCAAGGG CTTCTTCATC AACCAGTCGA CAGACGACAC CTTTAATCCC 
AAGCCCGACA ATCCAAGCCA CCTCAGCCAC TCGCTGATCG ATCTGCTTTC CCACATATCA 
CCATCGTTCC GACGTGCCTT CCAGACCATC CAGAAGCGAC GCACCATGCG TCATGCCTTC 
GAGCGTGTAG CCACGCCCTA TCAGGTGTAC CAGTGGGCAG CTCCCATCCT GGAGCACACA 
GTGGACGCTA TTCGTGCCGA GGATGCATTC AGCTCAAAAT TGGGCTACGA AGAGCACATC 
CCCGGACAGA CGCGCGACTG GAACGAAGAG CTACAGACGA CACGGGAACT GCCACGTAAG 
ACACTGCCGG AGCGCTTGCT GCGCGAGCGC GCCATCTTCA AGGTGCACGG GGATTTCGTG 
ACTGCCGCCA CGAGGGGCGC CATGGCCGTT ATCGACGGCA ATGTGCTGGC CATTAATCCC 
GGCGAGGACA CCAAGATGCA GATGTTCATT TGGAACAATA TCTTCTTCTC GATGGGCTTC 
GACGTAAGAG ACCATTACAA GGAACTGGGC GGCGATGCAG CCGCCTTTGT TGCCCCACGA 
TACGATCTGC ACGGCGTGCG TGTCTATAAT GCCGTTGATA TTGAAGGATT ATACACACTG 
GGAACCGTCG TCGTTGATTA TCGCGGCTAC CGTGTTACTG CCCAATCTAT TATTCCCGGA 
ATTCTGGAGC GGGAGCAGGA GCAAAGCGTC GTCTATGGCT CCATTGACTT TGGAAAGACG 
GTGCTCAGCC ATCCCAAATA CTTGGAGCTT CTTAGACAAG CCGGTAAGCA TCTGAAGATT 
TTGCCACACG TAGTGCTTAA CGAGCGGGAT GAACCCGTGG AGCTGTGCTC CTCGGTGGAG 
TGCAAGGGCA TTATTGGAAA CGATGGCCGA CACTACATCC TCGACTTATT GCGCACGTTC 
CCGCCAGACG TGAACTTCCT GAAGCTTCAG GACGTGCAGC TGAGCAAGGA ATTAGTTGAT 
ATGGGCTTCC CTATCGAGCA CAGGCACAAG TTGTGCTGCC TGCGTCAAGA GCTGCTGGAA 
GCTTTTATAG AGGATCGACA TGTTAACTTT ATCCGAATTG CTGCTGCGCG CCTTCAGCAG 
CTTACTACCA TAAAGCAGTC TGAAAAATCG GAGGCGAATC CTGTTCCAGC CTTAGAAGGA 
GCAGAAGCGG CAAGTAAAGT GAACGGAGCT GAAAAACCCG ATGACAAGGA GAAGAAAAAC 
GAAGAGGAGG AGAAGAAGGA GAGGTCCACT TCTGGCGAGG CAAGGGCCGC CGCCATTGTT 
AATGCCATCC GGGAAGCACA GTCCAATGTA GCTACTTCGA ATGAGGTGCA AGCTGCCGAG 
GTAGTAAAGC GGGCTTGCGC CGCCGTTGGA TCGCTGAAGG AGAAAGAGTT TGACTTCCGC 
TTCAACCCCG ACGTATTCTC GCCTGGCATT CGTCACGCGG ATGGCGAGGA GGGCACCTCG 
CTGGCCAAAC AGAAGGTGCT CGTGCAGGAA GCAGCCGAGT TTCTCGTCCT TAAGCAGATA 
CCCGCCTTTA TCAAGGAACA TATGTCCCAT TCATCGTCAC CAATCGATGG CCAGAGTCTA 
ACAGAATCTT TGCACAGTCA CGGCATCAAC GTGCGTTATT TGGGCAAGGT TATCAAAATT 
TTAAGCCAAA TGCCTCGCAT GGATTACCTG CATAGGATCG CTGTCCTCGA GCTGATCGTA 
CGGGCCACCA AGCACATCTA CTATACGTAC ATGCAGAATA CAGAGCCGCT GCACCTCTCT 
GCTGCCATCA GCCACTTCCT CAACTGTCTG CTGACCAACG GACCCGTCAA CCCGGCCGTG 
AGCAGCGAGG AGGCGCACAA GAAACGCGGC AACGGCGGTA AGCACAACAA GCACAAGTCC 
TCCAAGGGCG GAAAGGGTCA GCAGCAACAG CAGACGACAG GCAATCAGAA TGGCAGCTCG 
TCTGGCTCCT CCAATAGTTC ATCCGCATCC GATTGGACTC TGATGACGCC CCGATCCCTG 
TGGCAGCAAA TTCGTAAGGA GGCCAAGGTC TACTGGGACT GGGAATTGGA TTGTGACTCG 
ATTGAGACTG CGGTCTCCAA ATACGGCATC CTGCGTATCA GTCTGATGCG CGCCTTCTGC 
TTGAAGGTTG GTATTCAGGT GCTGCTGCGA GAGTACAACT TCGAGTCCAA GCACAAGCCA 
ACCTTTGGCG ACGACGACAT CGTCAATGTG TTCCCAATTG TGAAGCACAT TAGTCCCCGG 
GCAACCGACG CCTATAACTT CTACACCACG GGCCAGGCCA AGATCCAACA GGGCTTGTTT 
AAGGAGGGCT ATGAGCTAAT CAGCGGAGCA CTCAACCTGC TGAACAACGT ATTTGGCGCA 
TTGCATCAGG AAAACGGATC CTGCCTGCGC ATGCTGGCCA GGCTCAGCTA CCTCTTGGGC 
GATGCCCAAG ATGCTCTAGC GATCCAACAG CGAGCAGTGA TCATGAGCGA GCGGGTGAAC 
GGCATGGACC ACCCATCGAC CATATTGGAA TACACACACC TATCACTCTA TTCGTTTGCT 
AACGGACATG TGGGAATGTC TCTTAAGCTG CTGTACAGAG CTCGCTATCT GATGGTGCTC 
ATCTGTGGTG AAGATCATCC GGAAGTGGCA CTGATCGATA GCAACATCAG TTTGATTTTG 
CATGCGCTGG GCGAATACGA GTTGTCGCTG CGGTTTATCG AGCATGCCCT CAAGCTGAAC 
CTGAAGTACT TTGGCGACAA GGATATGCAC GTGGCACTCA GCTATCACCT GATGGCCCGC 
ACACAGTCCT GCATGGGCGA CTTCCGCTCA GCGTTGAATA ACGAGAAGGA AACGTACTCT 
TTCTACAAGT CTCAGCTTGG CGAGAACCAC GAGAAGACCA GGGACTCGGC CGAGTGCCTG 
CGTCTTTTGA CCCAGCAAGC TGTGCTGCTG CAGCGCAAGA TGAACGATAT CTATTCCAGT 
GGCAAACTGA CCAGTGACCT GCCGCCCATC CACATCACAC CACCGTCGAT GGGCTCGGTC 
CTGGACATGT TGAACACCAT CAATGGCATT CTGTTTGTAA AGATTAGCCG AAAAGACATT 
GTCAAGGTGC GCAGTGAAAT TGAAAAGCAT TTCAAGACCG ATAGCACCGA GAACGAGGTG 
AATGATGCTA TCAACTCCAT TGTGGCTGCT GCTAACAACA ATGGCGAGGC CGAGGATGCG 
GTATCCAAGG ATATAAAGGA GCAGCCAGAA GCAGGCAAGC AGCTAACAAA TGGCGACAAG 
GCGGCAGCCA CTGAGGCGAC TTCAAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261021.1
CDS	530..4399
Translation	MELVQEIHQLLMDREETCHRTCFSLQLDNVTLDNFAELKSINNLEQGSTIKVVEEPYTMREARIHVRHVRDLLKNLDPADAYNGIDCTSLTYLNTITQGDLLDKKRTRPDSVDCTPPEYVTPGVSDPPILPLHPNVKNAKGPQALKVLTTSAWNPPPGPRKLHGDLMYLYVVTMEDKRFHISACSKGFFINQSTDDTFNPKPDNPSHLSHSLIDLLSHISPSFRRAFQTIQKRRTMRHAFERVATPYQVYQWAAPILEHTVDAIRAEDAFSSKLGYEEHIPGQTRDWNEELQTTRELPRKTLPERLLRERAIFKVHGDFVTAATRGAMAVIDGNVLAINPGEDTKMQMFIWNNIFFSMGFDVRDHYKELGGDAAAFVAPRYDLHGVRVYNAVDIEGLYTLGTVVVDYRGYRVTAQSIIPGILEREQEQSVVYGSIDFGKTVLSHPKYLELLRQAGKHLKILPHVVLNERDEPVELCSSVECKGIIGNDGRHYILDLLRTFPPDVNFLKLQDVQLSKELVDMGFPIEHRHKLCCLRQELLEAFIEDRHVNFIRIAAARLQQLTTIKQSEKSEANPVPALEGAEAASKVNGAEKPDDKEKKNEEEEKKERSTSGEARAAAIVNAIREAQSNVATSNEVQAAEVVKRACAAVGSLKEKEFDFRFNPDVFSPGIRHADGEEGTSLAKQKVLVQEAAEFLVLKQIPAFIKEHMSHSSSPIDGQSLTESLHSHGINVRYLGKVIKILSQMPRMDYLHRIAVLELIVRATKHIYYTYMQNTEPLHLSAAISHFLNCLLTNGPVNPAVSSEEAHKKRGNGGKHNKHKSSKGGKGQQQQQTTGNQNGSSSGSSNSSSASDWTLMTPRSLWQQIRKEAKVYWDWELDCDSIETAVSKYGILRISLMRAFCLKVGIQVLLREYNFESKHKPTFGDDDIVNVFPIVKHISPRATDAYNFYTTGQAKIQQGLFKEGYELISGALNLLNNVFGALHQENGSCLRMLARLSYLLGDAQDALAIQQRAVIMSERVNGMDHPSTILEYTHLSLYSFANGHVGMSLKLLYRARYLMVLICGEDHPEVALIDSNISLILHALGEYELSLRFIEHALKLNLKYFGDKDMHVALSYHLMARTQSCMGDFRSALNNEKETYSFYKSQLGENHEKTRDSAECLRLLTQQAVLLQRKMNDIYSSGKLTSDLPPIHITPPSMGSVLDMLNTINGILFVKISRKDIVKVRSEIEKHFKTDSTENEVNDAINSIVAAANNNGEAEDAVSKDIKEQPEAGKQLTNGDKAAATEATSS

Target ORF information:

RefSeq Version	NM_001274092.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster clueless (clu), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274092.1

ORF Insert Sequence:

ATGGAGCTGG TGCAGGAAAT CCACCAGCTG CTGATGGACC GCGAAGAGAC GTGCCATCGT 
ACATGCTTCT CGCTGCAGCT GGACAACGTG ACGCTCGACA ATTTCGCCGA ACTCAAGTCA 
ATCAATAACC TTGAGCAGGG CTCGACGATC AAAGTGGTAG AGGAGCCGTA CACCATGCGG 
GAGGCCCGTA TTCATGTGCG CCATGTCCGT GATCTTCTCA AAAACCTTGA CCCGGCCGAC 
GCGTACAATG GCATTGACTG TACTTCGCTG ACCTACTTAA ACACGATCAC TCAGGGTGAT 
TTGCTGGACA AGAAGAGGAC GCGACCGGAC TCCGTGGATT GCACTCCGCC GGAGTATGTG 
ACTCCGGGTG TAAGTGATCC GCCAATTCTG CCATTGCATC CAAATGTGAA GAACGCCAAG 
GGACCGCAGG CTCTCAAGGT ACTGACTACA TCCGCCTGGA ACCCGCCACC CGGTCCTCGC 
AAGCTGCATG GTGACCTCAT GTATCTGTAC GTTGTCACCA TGGAGGATAA ACGATTCCAC 
ATCTCAGCCT GTTCCAAGGG CTTCTTCATC AACCAGTCGA CAGACGACAC CTTTAATCCC 
AAGCCCGACA ATCCAAGCCA CCTCAGCCAC TCGCTGATCG ATCTGCTTTC CCACATATCA 
CCATCGTTCC GACGTGCCTT CCAGACCATC CAGAAGCGAC GCACCATGCG TCATGCCTTC 
GAGCGTGTAG CCACGCCCTA TCAGGTGTAC CAGTGGGCAG CTCCCATCCT GGAGCACACA 
GTGGACGCTA TTCGTGCCGA GGATGCATTC AGCTCAAAAT TGGGCTACGA AGAGCACATC 
CCCGGACAGA CGCGCGACTG GAACGAAGAG CTACAGACGA CACGGGAACT GCCACGTAAG 
ACACTGCCGG AGCGCTTGCT GCGCGAGCGC GCCATCTTCA AGGTGCACGG GGATTTCGTG 
ACTGCCGCCA CGAGGGGCGC CATGGCCGTT ATCGACGGCA ATGTGCTGGC CATTAATCCC 
GGCGAGGACA CCAAGATGCA GATGTTCATT TGGAACAATA TCTTCTTCTC GATGGGCTTC 
GACGTAAGAG ACCATTACAA GGAACTGGGC GGCGATGCAG CCGCCTTTGT TGCCCCACGA 
TACGATCTGC ACGGCGTGCG TGTCTATAAT GCCGTTGATA TTGAAGGATT ATACACACTG 
GGAACCGTCG TCGTTGATTA TCGCGGCTAC CGTGTTACTG CCCAATCTAT TATTCCCGGA 
ATTCTGGAGC GGGAGCAGGA GCAAAGCGTC GTCTATGGCT CCATTGACTT TGGAAAGACG 
GTGCTCAGCC ATCCCAAATA CTTGGAGCTT CTTAGACAAG CCGGTAAGCA TCTGAAGATT 
TTGCCACACG TAGTGCTTAA CGAGCGGGAT GAACCCGTGG AGCTGTGCTC CTCGGTGGAG 
TGCAAGGGCA TTATTGGAAA CGATGGCCGA CACTACATCC TCGACTTATT GCGCACGTTC 
CCGCCAGACG TGAACTTCCT GAAGCTTCAG GACGTGCAGC TGAGCAAGGA ATTAGTTGAT 
ATGGGCTTCC CTATCGAGCA CAGGCACAAG TTGTGCTGCC TGCGTCAAGA GCTGCTGGAA 
GCTTTTATAG AGGATCGACA TGTTAACTTT ATCCGAATTG CTGCTGCGCG CCTTCAGCAG 
CTTACTACCA TAAAGCAGTC TGAAAAATCG GAGGCGAATC CTGTTCCAGC CTTAGAAGGA 
GCAGAAGCGG CAAGTAAAGT GAACGGAGCT GAAAAACCCG ATGACAAGGA GAAGAAAAAC 
GAAGAGGAGG AGAAGAAGGA GAGGTCCACT TCTGGCGAGG CAAGGGCCGC CGCCATTGTT 
AATGCCATCC GGGAAGCACA GTCCAATGTA GCTACTTCGA ATGAGGTGCA AGCTGCCGAG 
GTAGTAAAGC GGGCTTGCGC CGCCGTTGGA TCGCTGAAGG AGAAAGAGTT TGACTTCCGC 
TTCAACCCCG ACGTATTCTC GCCTGGCATT CGTCACGCGG ATGGCGAGGA GGGCACCTCG 
CTGGCCAAAC AGAAGGTGCT CGTGCAGGAA GCAGCCGAGT TTCTCGTCCT TAAGCAGATA 
CCCGCCTTTA TCAAGGAACA TATGTCCCAT TCATCGTCAC CAATCGATGG CCAGAGTCTA 
ACAGAATCTT TGCACAGTCA CGGCATCAAC GTGCGTTATT TGGGCAAGGT TATCAAAATT 
TTAAGCCAAA TGCCTCGCAT GGATTACCTG CATAGGATCG CTGTCCTCGA GCTGATCGTA 
CGGGCCACCA AGCACATCTA CTATACGTAC ATGCAGAATA CAGAGCCGCT GCACCTCTCT 
GCTGCCATCA GCCACTTCCT CAACTGTCTG CTGACCAACG GACCCGTCAA CCCGGCCGTG 
AGCAGCGAGG AGGCGCACAA GAAACGCGGC AACGGCGGTA AGCACAACAA GCACAAGTCC 
TCCAAGGGCG GAAAGGGTCA GCAGCAACAG CAGACGACAG GCAATCAGAA TGGCAGCTCG 
TCTGGCTCCT CCAATAGTTC ATCCGCATCC GATTGGACTC TGATGACGCC CCGATCCCTG 
TGGCAGCAAA TTCGTAAGGA GGCCAAGGTC TACTGGGACT GGGAATTGGA TTGTGACTCG 
ATTGAGACTG CGGTCTCCAA ATACGGCATC CTGCGTATCA GTCTGATGCG CGCCTTCTGC 
TTGAAGGTTG GTATTCAGGT GCTGCTGCGA GAGTACAACT TCGAGTCCAA GCACAAGCCA 
ACCTTTGGCG ACGACGACAT CGTCAATGTG TTCCCAATTG TGAAGCACAT TAGTCCCCGG 
GCAACCGACG CCTATAACTT CTACACCACG GGCCAGGCCA AGATCCAACA GGGCTTGTTT 
AAGGAGGGCT ATGAGCTAAT CAGCGGAGCA CTCAACCTGC TGAACAACGT ATTTGGCGCA 
TTGCATCAGG AAAACGGATC CTGCCTGCGC ATGCTGGCCA GGCTCAGCTA CCTCTTGGGC 
GATGCCCAAG ATGCTCTAGC GATCCAACAG CGAGCAGTGA TCATGAGCGA GCGGGTGAAC 
GGCATGGACC ACCCATCGAC CATATTGGAA TACACACACC TATCACTCTA TTCGTTTGCT 
AACGGACATG TGGGAATGTC TCTTAAGCTG CTGTACAGAG CTCGCTATCT GATGGTGCTC 
ATCTGTGGTG AAGATCATCC GGAAGTGGCA CTGATCGATA GCAACATCAG TTTGATTTTG 
CATGCGCTGG GCGAATACGA GTTGTCGCTG CGGTTTATCG AGCATGCCCT CAAGCTGAAC 
CTGAAGTACT TTGGCGACAA GGATATGCAC GTGGCACTCA GCTATCACCT GATGGCCCGC 
ACACAGTCCT GCATGGGCGA CTTCCGCTCA GCGTTGAATA ACGAGAAGGA AACGTACTCT 
TTCTACAAGT CTCAGCTTGG CGAGAACCAC GAGAAGACCA GGGACTCGGC CGAGTGCCTG 
CGTCTTTTGA CCCAGCAAGC TGTGCTGCTG CAGCGCAAGA TGAACGATAT CTATTCCAGT 
GGCAAACTGA CCAGTGACCT GCCGCCCATC CACATCACAC CACCGTCGAT GGGCTCGGTC 
CTGGACATGT TGAACACCAT CAATGGCATT CTGTTTGTAA AGATTAGCCG AAAAGACATT 
GTCAAGGTGC GCAGTGAAAT TGAAAAGCAT TTCAAGACCG ATAGCACCGA GAACGAGGTG 
AATGATGCTA TCAACTCCAT TGTGGCTGCT GCTAACAACA ATGGCGAGGC CGAGGATGCG 
GTATCCAAGG ATATAAAGGA GCAGCCAGAA GCAGGCAAGC AGCTAACAAA TGGCGACAAG 
GCGGCAGCCA CTGAGGCGAC TTCAAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

clu ( NM_137251.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611095.1 Drosophila melanogaster clueless (clu), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa10180
Clone ID Related Accession (Same CDS sequence)	NM_137251.3
Accession Version	NM_137251.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4347bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	clueless, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_137251.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGCTTG AAACGGAGGC GAAGAATTCA AATGCAACGG CCACGGGCGA TGCAACAGCA 
ACAGCAACAA AGGCCAGCGG CAAGGCCAAG GAGAACAACA ACACGGCCGG CGGCAAAAAG 
AACCTGAATC CGAATTCGAA TCAGCAGAAT TCGAATCAGA ACTTGGTCAA TGGCAATGGC 
ACGGCTGCCG ACGGGCCGGC TGCGAAGAAG AAAGGCAAGA AGAACCGCAA CAAAAGCCCA 
ACTGAACCCA CAACCGAAGC CGTTCTGAGC AACGGACACG CCGAGAAGCC CACAGTGGTG 
GATGCAGTTG AGGACAATGC CGATACCAAT GCCAATGTGG AGAAACCGCA GGAAGGAGGA 
GCACCGGATG CTGAGGCTGA TGGTGATGAC ATTGATCTGG ACGCTCTGCA GGACATTGGC 
ATCACGGTGA ACATCAGCAG TCCCGGCGCC GATCTGCTCT GCGTGCAGCT GTCCAGCATG 
GAGCTGGTGC AGGAAATCCA CCAGCTGCTG ATGGACCGCG AAGAGACGTG CCATCGTACA 
TGCTTCTCGC TGCAGCTGGA CAACGTGACG CTCGACAATT TCGCCGAACT CAAGTCAATC 
AATAACCTTG AGCAGGGCTC GACGATCAAA GTGGTAGAGG AGCCGTACAC CATGCGGGAG 
GCCCGTATTC ATGTGCGCCA TGTCCGTGAT CTTCTCAAAA ACCTTGACCC GGCCGACGCG 
TACAATGGCA TTGACTGTAC TTCGCTGACC TACTTAAACA CGATCACTCA GGGTGATTTG 
CTGGACAAGA AGAGGACGCG ACCGGACTCC GTGGATTGCA CTCCGCCGGA GTATGTGACT 
CCGGGTGTAA GTGATCCGCC AATTCTGCCA TTGCATCCAA ATGTGAAGAA CGCCAAGGGA 
CCGCAGGCTC TCAAGGTACT GACTACATCC GCCTGGAACC CGCCACCCGG TCCTCGCAAG 
CTGCATGGTG ACCTCATGTA TCTGTACGTT GTCACCATGG AGGATAAACG ATTCCACATC 
TCAGCCTGTT CCAAGGGCTT CTTCATCAAC CAGTCGACAG ACGACACCTT TAATCCCAAG 
CCCGACAATC CAAGCCACCT CAGCCACTCG CTGATCGATC TGCTTTCCCA CATATCACCA 
TCGTTCCGAC GTGCCTTCCA GACCATCCAG AAGCGACGCA CCATGCGTCA TGCCTTCGAG 
CGTGTAGCCA CGCCCTATCA GGTGTACCAG TGGGCAGCTC CCATCCTGGA GCACACAGTG 
GACGCTATTC GTGCCGAGGA TGCATTCAGC TCAAAATTGG GCTACGAAGA GCACATCCCC 
GGACAGACGC GCGACTGGAA CGAAGAGCTA CAGACGACAC GGGAACTGCC ACGTAAGACA 
CTGCCGGAGC GCTTGCTGCG CGAGCGCGCC ATCTTCAAGG TGCACGGGGA TTTCGTGACT 
GCCGCCACGA GGGGCGCCAT GGCCGTTATC GACGGCAATG TGCTGGCCAT TAATCCCGGC 
GAGGACACCA AGATGCAGAT GTTCATTTGG AACAATATCT TCTTCTCGAT GGGCTTCGAC 
GTAAGAGACC ATTACAAGGA ACTGGGCGGC GATGCAGCCG CCTTTGTTGC CCCACGATAC 
GATCTGCACG GCGTGCGTGT CTATAATGCC GTTGATATTG AAGGATTATA CACACTGGGA 
ACCGTCGTCG TTGATTATCG CGGCTACCGT GTTACTGCCC AATCTATTAT TCCCGGAATT 
CTGGAGCGGG AGCAGGAGCA AAGCGTCGTC TATGGCTCCA TTGACTTTGG AAAGACGGTG 
CTCAGCCATC CCAAATACTT GGAGCTTCTT AGACAAGCCG GTAAGCATCT GAAGATTTTG 
CCACACGTAG TGCTTAACGA GCGGGATGAA CCCGTGGAGC TGTGCTCCTC GGTGGAGTGC 
AAGGGCATTA TTGGAAACGA TGGCCGACAC TACATCCTCG ACTTATTGCG CACGTTCCCG 
CCAGACGTGA ACTTCCTGAA GCTTCAGGAC GTGCAGCTGA GCAAGGAATT AGTTGATATG 
GGCTTCCCTA TCGAGCACAG GCACAAGTTG TGCTGCCTGC GTCAAGAGCT GCTGGAAGCT 
TTTATAGAGG ATCGACATGT TAACTTTATC CGAATTGCTG CTGCGCGCCT TCAGCAGCTT 
ACTACCATAA AGCAGTCTGA AAAATCGGAG GCGAATCCTG TTCCAGCCTT AGAAGGAGCA 
GAAGCGGCAA GTAAAGTGAA CGGAGCTGAA AAACCCGATG ACAAGGAGAA GAAAAACGAA 
GAGGAGGAGA AGAAGGAGAG GTCCACTTCT GGCGAGGCAA GGGCCGCCGC CATTGTTAAT 
GCCATCCGGG AAGCACAGTC CAATGTAGCT ACTTCGAATG AGGTGCAAGC TGCCGAGGTA 
GTAAAGCGGG CTTGCGCCGC CGTTGGATCG CTGAAGGAGA AAGAGTTTGA CTTCCGCTTC 
AACCCCGACG TATTCTCGCC TGGCATTCGT CACGCGGATG GCGAGGAGGG CACCTCGCTG 
GCCAAACAGA AGGTGCTCGT GCAGGAAGCA GCCGAGTTTC TCGTCCTTAA GCAGATACCC 
GCCTTTATCA AGGAACATAT GTCCCATTCA TCGTCACCAA TCGATGGCCA GAGTCTAACA 
GAATCTTTGC ACAGTCACGG CATCAACGTG CGTTATTTGG GCAAGGTTAT CAAAATTTTA 
AGCCAAATGC CTCGCATGGA TTACCTGCAT AGGATCGCTG TCCTCGAGCT GATCGTACGG 
GCCACCAAGC ACATCTACTA TACGTACATG CAGAATACAG AGCCGCTGCA CCTCTCTGCT 
GCCATCAGCC ACTTCCTCAA CTGTCTGCTG ACCAACGGAC CCGTCAACCC GGCCGTGAGC 
AGCGAGGAGG CGCACAAGAA ACGCGGCAAC GGCGGTAAGC ACAACAAGCA CAAGTCCTCC 
AAGGGCGGAA AGGGTCAGCA GCAACAGCAG ACGACAGGCA ATCAGAATGG CAGCTCGTCT 
GGCTCCTCCA ATAGTTCATC CGCATCCGAT TGGACTCTGA TGACGCCCCG ATCCCTGTGG 
CAGCAAATTC GTAAGGAGGC CAAGGTCTAC TGGGACTGGG AATTGGATTG TGACTCGATT 
GAGACTGCGG TCTCCAAATA CGGCATCCTG CGTATCAGTC TGATGCGCGC CTTCTGCTTG 
AAGGTTGGTA TTCAGGTGCT GCTGCGAGAG TACAACTTCG AGTCCAAGCA CAAGCCAACC 
TTTGGCGACG ACGACATCGT CAATGTGTTC CCAATTGTGA AGCACATTAG TCCCCGGGCA 
ACCGACGCCT ATAACTTCTA CACCACGGGC CAGGCCAAGA TCCAACAGGG CTTGTTTAAG 
GAGGGCTATG AGCTAATCAG CGGAGCACTC AACCTGCTGA ACAACGTATT TGGCGCATTG 
CATCAGGAAA ACGGATCCTG CCTGCGCATG CTGGCCAGGC TCAGCTACCT CTTGGGCGAT 
GCCCAAGATG CTCTAGCGAT CCAACAGCGA GCAGTGATCA TGAGCGAGCG GGTGAACGGC 
ATGGACCACC CATCGACCAT ATTGGAATAC ACACACCTAT CACTCTATTC GTTTGCTAAC 
GGACATGTGG GAATGTCTCT TAAGCTGCTG TACAGAGCTC GCTATCTGAT GGTGCTCATC 
TGTGGTGAAG ATCATCCGGA AGTGGCACTG ATCGATAGCA ACATCAGTTT GATTTTGCAT 
GCGCTGGGCG AATACGAGTT GTCGCTGCGG TTTATCGAGC ATGCCCTCAA GCTGAACCTG 
AAGTACTTTG GCGACAAGGA TATGCACGTG GCACTCAGCT ATCACCTGAT GGCCCGCACA 
CAGTCCTGCA TGGGCGACTT CCGCTCAGCG TTGAATAACG AGAAGGAAAC GTACTCTTTC 
TACAAGTCTC AGCTTGGCGA GAACCACGAG AAGACCAGGG ACTCGGCCGA GTGCCTGCGT 
CTTTTGACCC AGCAAGCTGT GCTGCTGCAG CGCAAGATGA ACGATATCTA TTCCAGTGGC 
AAACTGACCA GTGACCTGCC GCCCATCCAC ATCACACCAC CGTCGATGGG CTCGGTCCTG 
GACATGTTGA ACACCATCAA TGGCATTCTG TTTGTAAAGA TTAGCCGAAA AGACATTGTC 
AAGGTGCGCA GTGAAATTGA AAAGCATTTC AAGACCGATA GCACCGAGAA CGAGGTGAAT 
GATGCTATCA ACTCCATTGT GGCTGCTGCT AACAACAATG GCGAGGCCGA GGATGCGGTA 
TCCAAGGATA TAAAGGAGCA GCCAGAAGCA GGCAAGCAGC TAACAAATGG CGACAAGGCG 
GCAGCCACTG AGGCGACTTC AAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611095.1
CDS	340..4686
Translation	MALETEAKNSNATATGDATATATKASGKAKENNNTAGGKKNLNPNSNQQNSNQNLVNGNGTAADGPAAKKKGKKNRNKSPTEPTTEAVLSNGHAEKPTVVDAVEDNADTNANVEKPQEGGAPDAEADGDDIDLDALQDIGITVNISSPGADLLCVQLSSMELVQEIHQLLMDREETCHRTCFSLQLDNVTLDNFAELKSINNLEQGSTIKVVEEPYTMREARIHVRHVRDLLKNLDPADAYNGIDCTSLTYLNTITQGDLLDKKRTRPDSVDCTPPEYVTPGVSDPPILPLHPNVKNAKGPQALKVLTTSAWNPPPGPRKLHGDLMYLYVVTMEDKRFHISACSKGFFINQSTDDTFNPKPDNPSHLSHSLIDLLSHISPSFRRAFQTIQKRRTMRHAFERVATPYQVYQWAAPILEHTVDAIRAEDAFSSKLGYEEHIPGQTRDWNEELQTTRELPRKTLPERLLRERAIFKVHGDFVTAATRGAMAVIDGNVLAINPGEDTKMQMFIWNNIFFSMGFDVRDHYKELGGDAAAFVAPRYDLHGVRVYNAVDIEGLYTLGTVVVDYRGYRVTAQSIIPGILEREQEQSVVYGSIDFGKTVLSHPKYLELLRQAGKHLKILPHVVLNERDEPVELCSSVECKGIIGNDGRHYILDLLRTFPPDVNFLKLQDVQLSKELVDMGFPIEHRHKLCCLRQELLEAFIEDRHVNFIRIAAARLQQLTTIKQSEKSEANPVPALEGAEAASKVNGAEKPDDKEKKNEEEEKKERSTSGEARAAAIVNAIREAQSNVATSNEVQAAEVVKRACAAVGSLKEKEFDFRFNPDVFSPGIRHADGEEGTSLAKQKVLVQEAAEFLVLKQIPAFIKEHMSHSSSPIDGQSLTESLHSHGINVRYLGKVIKILSQMPRMDYLHRIAVLELIVRATKHIYYTYMQNTEPLHLSAAISHFLNCLLTNGPVNPAVSSEEAHKKRGNGGKHNKHKSSKGGKGQQQQQTTGNQNGSSSGSSNSSSASDWTLMTPRSLWQQIRKEAKVYWDWELDCDSIETAVSKYGILRISLMRAFCLKVGIQVLLREYNFESKHKPTFGDDDIVNVFPIVKHISPRATDAYNFYTTGQAKIQQGLFKEGYELISGALNLLNNVFGALHQENGSCLRMLARLSYLLGDAQDALAIQQRAVIMSERVNGMDHPSTILEYTHLSLYSFANGHVGMSLKLLYRARYLMVLICGEDHPEVALIDSNISLILHALGEYELSLRFIEHALKLNLKYFGDKDMHVALSYHLMARTQSCMGDFRSALNNEKETYSFYKSQLGENHEKTRDSAECLRLLTQQAVLLQRKMNDIYSSGKLTSDLPPIHITPPSMGSVLDMLNTINGILFVKISRKDIVKVRSEIEKHFKTDSTENEVNDAINSIVAAANNNGEAEDAVSKDIKEQPEAGKQLTNGDKAAATEATSS

Target ORF information:

RefSeq Version	NM_137251.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster clueless (clu), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137251.3

ORF Insert Sequence:

ATGGCGCTTG AAACGGAGGC GAAGAATTCA AATGCAACGG CCACGGGCGA TGCAACAGCA 
ACAGCAACAA AGGCCAGCGG CAAGGCCAAG GAGAACAACA ACACGGCCGG CGGCAAAAAG 
AACCTGAATC CGAATTCGAA TCAGCAGAAT TCGAATCAGA ACTTGGTCAA TGGCAATGGC 
ACGGCTGCCG ACGGGCCGGC TGCGAAGAAG AAAGGCAAGA AGAACCGCAA CAAAAGCCCA 
ACTGAACCCA CAACCGAAGC CGTTCTGAGC AACGGACACG CCGAGAAGCC CACAGTGGTG 
GATGCAGTTG AGGACAATGC CGATACCAAT GCCAATGTGG AGAAACCGCA GGAAGGAGGA 
GCACCGGATG CTGAGGCTGA TGGTGATGAC ATTGATCTGG ACGCTCTGCA GGACATTGGC 
ATCACGGTGA ACATCAGCAG TCCCGGCGCC GATCTGCTCT GCGTGCAGCT GTCCAGCATG 
GAGCTGGTGC AGGAAATCCA CCAGCTGCTG ATGGACCGCG AAGAGACGTG CCATCGTACA 
TGCTTCTCGC TGCAGCTGGA CAACGTGACG CTCGACAATT TCGCCGAACT CAAGTCAATC 
AATAACCTTG AGCAGGGCTC GACGATCAAA GTGGTAGAGG AGCCGTACAC CATGCGGGAG 
GCCCGTATTC ATGTGCGCCA TGTCCGTGAT CTTCTCAAAA ACCTTGACCC GGCCGACGCG 
TACAATGGCA TTGACTGTAC TTCGCTGACC TACTTAAACA CGATCACTCA GGGTGATTTG 
CTGGACAAGA AGAGGACGCG ACCGGACTCC GTGGATTGCA CTCCGCCGGA GTATGTGACT 
CCGGGTGTAA GTGATCCGCC AATTCTGCCA TTGCATCCAA ATGTGAAGAA CGCCAAGGGA 
CCGCAGGCTC TCAAGGTACT GACTACATCC GCCTGGAACC CGCCACCCGG TCCTCGCAAG 
CTGCATGGTG ACCTCATGTA TCTGTACGTT GTCACCATGG AGGATAAACG ATTCCACATC 
TCAGCCTGTT CCAAGGGCTT CTTCATCAAC CAGTCGACAG ACGACACCTT TAATCCCAAG 
CCCGACAATC CAAGCCACCT CAGCCACTCG CTGATCGATC TGCTTTCCCA CATATCACCA 
TCGTTCCGAC GTGCCTTCCA GACCATCCAG AAGCGACGCA CCATGCGTCA TGCCTTCGAG 
CGTGTAGCCA CGCCCTATCA GGTGTACCAG TGGGCAGCTC CCATCCTGGA GCACACAGTG 
GACGCTATTC GTGCCGAGGA TGCATTCAGC TCAAAATTGG GCTACGAAGA GCACATCCCC 
GGACAGACGC GCGACTGGAA CGAAGAGCTA CAGACGACAC GGGAACTGCC ACGTAAGACA 
CTGCCGGAGC GCTTGCTGCG CGAGCGCGCC ATCTTCAAGG TGCACGGGGA TTTCGTGACT 
GCCGCCACGA GGGGCGCCAT GGCCGTTATC GACGGCAATG TGCTGGCCAT TAATCCCGGC 
GAGGACACCA AGATGCAGAT GTTCATTTGG AACAATATCT TCTTCTCGAT GGGCTTCGAC 
GTAAGAGACC ATTACAAGGA ACTGGGCGGC GATGCAGCCG CCTTTGTTGC CCCACGATAC 
GATCTGCACG GCGTGCGTGT CTATAATGCC GTTGATATTG AAGGATTATA CACACTGGGA 
ACCGTCGTCG TTGATTATCG CGGCTACCGT GTTACTGCCC AATCTATTAT TCCCGGAATT 
CTGGAGCGGG AGCAGGAGCA AAGCGTCGTC TATGGCTCCA TTGACTTTGG AAAGACGGTG 
CTCAGCCATC CCAAATACTT GGAGCTTCTT AGACAAGCCG GTAAGCATCT GAAGATTTTG 
CCACACGTAG TGCTTAACGA GCGGGATGAA CCCGTGGAGC TGTGCTCCTC GGTGGAGTGC 
AAGGGCATTA TTGGAAACGA TGGCCGACAC TACATCCTCG ACTTATTGCG CACGTTCCCG 
CCAGACGTGA ACTTCCTGAA GCTTCAGGAC GTGCAGCTGA GCAAGGAATT AGTTGATATG 
GGCTTCCCTA TCGAGCACAG GCACAAGTTG TGCTGCCTGC GTCAAGAGCT GCTGGAAGCT 
TTTATAGAGG ATCGACATGT TAACTTTATC CGAATTGCTG CTGCGCGCCT TCAGCAGCTT 
ACTACCATAA AGCAGTCTGA AAAATCGGAG GCGAATCCTG TTCCAGCCTT AGAAGGAGCA 
GAAGCGGCAA GTAAAGTGAA CGGAGCTGAA AAACCCGATG ACAAGGAGAA GAAAAACGAA 
GAGGAGGAGA AGAAGGAGAG GTCCACTTCT GGCGAGGCAA GGGCCGCCGC CATTGTTAAT 
GCCATCCGGG AAGCACAGTC CAATGTAGCT ACTTCGAATG AGGTGCAAGC TGCCGAGGTA 
GTAAAGCGGG CTTGCGCCGC CGTTGGATCG CTGAAGGAGA AAGAGTTTGA CTTCCGCTTC 
AACCCCGACG TATTCTCGCC TGGCATTCGT CACGCGGATG GCGAGGAGGG CACCTCGCTG 
GCCAAACAGA AGGTGCTCGT GCAGGAAGCA GCCGAGTTTC TCGTCCTTAA GCAGATACCC 
GCCTTTATCA AGGAACATAT GTCCCATTCA TCGTCACCAA TCGATGGCCA GAGTCTAACA 
GAATCTTTGC ACAGTCACGG CATCAACGTG CGTTATTTGG GCAAGGTTAT CAAAATTTTA 
AGCCAAATGC CTCGCATGGA TTACCTGCAT AGGATCGCTG TCCTCGAGCT GATCGTACGG 
GCCACCAAGC ACATCTACTA TACGTACATG CAGAATACAG AGCCGCTGCA CCTCTCTGCT 
GCCATCAGCC ACTTCCTCAA CTGTCTGCTG ACCAACGGAC CCGTCAACCC GGCCGTGAGC 
AGCGAGGAGG CGCACAAGAA ACGCGGCAAC GGCGGTAAGC ACAACAAGCA CAAGTCCTCC 
AAGGGCGGAA AGGGTCAGCA GCAACAGCAG ACGACAGGCA ATCAGAATGG CAGCTCGTCT 
GGCTCCTCCA ATAGTTCATC CGCATCCGAT TGGACTCTGA TGACGCCCCG ATCCCTGTGG 
CAGCAAATTC GTAAGGAGGC CAAGGTCTAC TGGGACTGGG AATTGGATTG TGACTCGATT 
GAGACTGCGG TCTCCAAATA CGGCATCCTG CGTATCAGTC TGATGCGCGC CTTCTGCTTG 
AAGGTTGGTA TTCAGGTGCT GCTGCGAGAG TACAACTTCG AGTCCAAGCA CAAGCCAACC 
TTTGGCGACG ACGACATCGT CAATGTGTTC CCAATTGTGA AGCACATTAG TCCCCGGGCA 
ACCGACGCCT ATAACTTCTA CACCACGGGC CAGGCCAAGA TCCAACAGGG CTTGTTTAAG 
GAGGGCTATG AGCTAATCAG CGGAGCACTC AACCTGCTGA ACAACGTATT TGGCGCATTG 
CATCAGGAAA ACGGATCCTG CCTGCGCATG CTGGCCAGGC TCAGCTACCT CTTGGGCGAT 
GCCCAAGATG CTCTAGCGAT CCAACAGCGA GCAGTGATCA TGAGCGAGCG GGTGAACGGC 
ATGGACCACC CATCGACCAT ATTGGAATAC ACACACCTAT CACTCTATTC GTTTGCTAAC 
GGACATGTGG GAATGTCTCT TAAGCTGCTG TACAGAGCTC GCTATCTGAT GGTGCTCATC 
TGTGGTGAAG ATCATCCGGA AGTGGCACTG ATCGATAGCA ACATCAGTTT GATTTTGCAT 
GCGCTGGGCG AATACGAGTT GTCGCTGCGG TTTATCGAGC ATGCCCTCAA GCTGAACCTG 
AAGTACTTTG GCGACAAGGA TATGCACGTG GCACTCAGCT ATCACCTGAT GGCCCGCACA 
CAGTCCTGCA TGGGCGACTT CCGCTCAGCG TTGAATAACG AGAAGGAAAC GTACTCTTTC 
TACAAGTCTC AGCTTGGCGA GAACCACGAG AAGACCAGGG ACTCGGCCGA GTGCCTGCGT 
CTTTTGACCC AGCAAGCTGT GCTGCTGCAG CGCAAGATGA ACGATATCTA TTCCAGTGGC 
AAACTGACCA GTGACCTGCC GCCCATCCAC ATCACACCAC CGTCGATGGG CTCGGTCCTG 
GACATGTTGA ACACCATCAA TGGCATTCTG TTTGTAAAGA TTAGCCGAAA AGACATTGTC 
AAGGTGCGCA GTGAAATTGA AAAGCATTTC AAGACCGATA GCACCGAGAA CGAGGTGAAT 
GATGCTATCA ACTCCATTGT GGCTGCTGCT AACAACAATG GCGAGGCCGA GGATGCGGTA 
TCCAAGGATA TAAAGGAGCA GCCAGAAGCA GGCAAGCAGC TAACAAATGG CGACAAGGCG 
GCAGCCACTG AGGCGACTTC AAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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clu cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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